Publications by authors named "Caroline Pagneux"

2 Publications

  • Page 1 of 1

Trichinella spiralis - New method for sample preparation and objective detection of specific antigens using a chemiluminescence immunoassay.

Vet Parasitol X 2020 Dec 8;4:100033. Epub 2020 Oct 8.

Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Seekamp 31, 23560 Lübeck, Germany.

The parasitic roundworm is most commonly transmitted to humans through consumption of raw or undercooked meat of infected pigs or game. To prevent human infection, slaughterhouses perform meat safety surveillance using the gold standard "Magnetic Stirrer Method". We introduce a fast and objective method using automated detection of specific antigens by a newly developed immunoassay based on chemiluminescence (ChLIA). Panel A comprised muscle tissue samples from non-infected pigs (n = 37). Panel B comprised muscle tissue samples from non-infected pigs spiked with different amounts of larvae without collagen capsules (n = 56). Panel C contained muscle tissue samples from experimentally infected pigs including larvae encapsulated in collagen (n = 32). Each sample was shredded with PBS buffer in a knife mill, destroying larvae. Following centrifugation, the supernatant (muscle tissue extract containing released excretory and secretory antigens) was used for specific antigen detection by the new Trichinella ChLIA. The overall accuracy of the Trichinella ChLIA was 97.6 %. The specificity of the Trichinella ChLIA was 100 % (panel A). The sensitivity in samples from experimentally infected pigs was 100 % representing a detection limit of 0.01 larvae per gram. Cross-reactivity with parasites other than . was not observed. This new meat inspection method for the detection of antigens presents high specificity and high sensitivity, especially in truly infected samples. In contrast to the gold standard, this new approach to meat safety surveillance does not require longsome digestion or microscopy by trained personnel.
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http://dx.doi.org/10.1016/j.vpoa.2020.100033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711141PMC
December 2020

Clinical cases of Bluetongue serotype 8 in calves in France in the 2018-2019 winter.

Transbound Emerg Dis 2020 May 8;67(3):1401-1405. Epub 2020 Jan 8.

Epidemiology Unit, Laboratory for Animal Health, ANSES, University Paris Est, Maisons-Alfort, France.

Bluetongue virus serotype 8 (BTV-8) caused an epizootic in Europe in 2006/09. Transplacental transmission of BTV-8 was demonstrated leading to abortions, congenital malformations or nervous clinical signs in newborn calves. BTV-8 re-emerged in France in 2015. Although the re-emergent strain is nearly genetically identical to the one that had circulated in 2006/2009, it has caused very few clinical cases. However, from mid-December 2018 to April 2019, cases of calves with congenital malformations or displaying nervous clinical signs occurred in some departments (French administrative unit) in mainland France. Blood samples from these animals were sent to local laboratories, and the positive ones were confirmed at the French Bluetongue reference laboratory (BT-NRL). Out of 580 samples found positive at the local laboratories, 544 were confirmed as RT-PCR BTV-8 positive. The 36 samples found positive in the local laboratories and negative in the BT-NRL were all at the limit of RT-PCR detection. Hundred eighty-eight of the confirmed samples were also tested for the presence of Schmallenberg virus (SBV) and bovine virus diarrhoea virus (BVDV) infection: 4 were found positive for BVDV and none for SBV. The main clinical signs recorded for 244 calves, for which a reporting form was completed by veterinarians, included nervous clinical signs (81%), amaurosis (72%) and decrease/ no suckling reflex (40%). Hydranencephaly and microphthalmia were reported in 19 calves out of 27 in which a necropsy was practiced after death or euthanasia. These results indicate that the re-emergent strain of BTV-8 can cross the transplacental barrier and cause congenital malformations or nervous clinical signs in calves.
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http://dx.doi.org/10.1111/tbed.13466DOI Listing
May 2020