Carlos L Perez, MD - Instituto de Ciencias Básicas y Preclíncas Victoria de Girón, Universidad de Ciencias Médicas de La Habana.

Carlos L Perez

MD

Instituto de Ciencias Básicas y Preclíncas Victoria de Girón, Universidad de Ciencias Médicas de La Habana.

La Habana | Cuba

ORCID logohttps://orcid.org/0000-0002-7063-6843

Carlos L Perez, MD - Instituto de Ciencias Básicas y Preclíncas Victoria de Girón, Universidad de Ciencias Médicas de La Habana.

Carlos L Perez

MD

Introduction

Primary Affiliation: Instituto de Ciencias Básicas y Preclíncas Victoria de Girón, Universidad de Ciencias Médicas de La Habana. - La Habana , Cuba

Experience

Oct 2017
University of Antwerp

Department of Biomedical Sciences
Sep 2011
Universidad de Ciencias Médicas de La Habana (UCMH)

Instituto de Ciencias Basicas y Preclinicas ¨Victoria de Giron¨ (ICBP)

Publications

10Publications

195Reads

78Profile Views

2PubMed Central Citations

Glucocorticoid receptor antagonism overcomes resistance to BRAF inhibition in BRAFV600E-mutated metastatic melanoma.

TY - JOUR T1 - Glucocorticoid receptor antagonism overcomes resistance to BRAF inhibition in BRAF V600E -mutated metastatic melanoma A1 - Estrela, José M A1 - Salvador, Rosario A1 - Marchio, Patricia A1 - Valles, Soraya L A1 - López-blanch, Rafael A1 - Rivera, Pilar A1 - Benlloch, María A1

American journal of cancer research

Clinical applications of glucocorticoids (GC) in Oncology are dependent on their pro-apoptotic action to treat lymphoproliferative cancers, and to alleviate side effects induced by chemotherapy and/or radiotherapy. However, the mechanism(s) by which GC may also promote tumor progression remains unclear. GC receptor (GR) knockdown decreases the antioxidant protection of highly metastatic B16-F10 melanoma cells. We hypothesize that a GR antagonist (RU486, mifepristone) could increase the efficacy of BRAF-related therapy in BRAFV600E-mutated metastatic melanoma. In vivo formed spontaneous skin tumors were reinoculated into nude mice to expand the metastases of different human BRAFV600E melanoma cells. The GR content of melanoma cell lines was measured by [3H]-labeled ligand binding assay. Nuclear Nrf2 and its transcription activity was investigated by RT-PCR, western blotting, and by measuring Nrf2- and redox state-related enzyme activities and metabolites. GR knockdown was achieved using lentivirus, and GR overexpression by transfection with the NR3C1 plasmid. shRNA-induced selective Bcl-xL, Mcl-1, AKT1 or NF-κB/p65 depletion was used to test the efficacy of vemurafenib (VMF) and RU486 against BRAFV600E-mutated metastatic melanoma. During early progression of skin melanoma metastases, RU486 and VMF induced a drastic metastases regression. However, treatment at an advanced stage of growth demonstrated the development of resistance to RU486 and VMF. This resistance was mechanistically linked to overexpression of specific proteins of the Bcl-2 family (Bcl-xL and Mcl-1 in our experimental models). We found that melanoma resistance is decreased if AKT and NF-κB signaling pathways are blocked. Our results highlight mechanisms by which metastatic melanoma cells adapt to survive.

https://europepmc.org/articles/PMC6943348

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December 2019

Xanthium strumarium´s xanthatins induces mitotic arrest and apoptosis in CT26WT colon carcinoma cells.

Phytomedicine 2019 Apr 17;57:236-244. Epub 2018 Dec 17.

Departamento de Farmacología, Instituto de Ciencias Biológicas (ICB) Universidad Federal de Minas Gerais (UFMG), Avda. Antonio Carlos 6627, Belo Horizonte, Minas Gerais, Brasil.

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http://dx.doi.org/10.1016/j.phymed.2018.12.019DOI Listing
April 2019
7 Reads
3.126 Impact Factor

In vitro modulation of the cytochrome P450 and ABCB1/P-glycoprotein activities of the aqueous extract of Allophylus cominia (L) Sw. leaves.

Drug Metab Pers Ther 2017 12;32(4):201-208

Departamento de Farmacología, Instituto de Ciencias del Mar (ICIMAR), Loma # 14, Vedado, Plaza de la Revolución, 10400, La Habana, Cuba, Phone: +537 8819812.

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http://dx.doi.org/10.1515/dmpt-2017-0020DOI Listing
December 2017
20 Reads

Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects.

Evid Based Complement Alternat Med 2015 3;2015:520598. Epub 2015 Nov 3.

Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), CP 70228, 04510 México, DF, Mexico.

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http://dx.doi.org/10.1155/2015/520598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647032PMC
November 2015
32 Reads
1 Citation
1.880 Impact Factor

Xanthium strumarium L. extracts produce DNA damage mediated by cytotoxicity in in vitro assays but does not induce micronucleus in mice.

Biomed Res Int 2014 15;2014:575197. Epub 2014 Jun 15.

Departamento de Biología Vegetal, Laboratorio de Toxicología Genética, Facultad de Biología, Universidad de la Habana, Calle 25, No. 455, e/ I y J, Vedado, C. Habana, Cuba.

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http://dx.doi.org/10.1155/2014/575197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4082875PMC
March 2015
108 Reads
1 Citation

Top co-authors

Idania Rodeiro
Idania Rodeiro

Università degli Studi di Pisa

4
Gisselle Fernández
Gisselle Fernández

Instituto de Ciencias Básicas y Preclinicas Victoria de Girón

2
Maria L Gonzalez
Maria L Gonzalez

Centro de Investigación y Desarrollo de Medicamentos (CIDEM)

2
Yasnay Hernandez
Yasnay Hernandez

Université de Nice-Sophia Antipolis

2
Ivones Hernandez
Ivones Hernandez

Centro de Bioproductos Marinos (CEBIMAR)

2
Tommaso Cornetta
Tommaso Cornetta

Roma Tre University

1
Mario Fiore
Mario Fiore

Institute of Molecular Biology and Pathology

1
Pasquale Stano
Pasquale Stano

Roma Tre University

1
Renata Cozzi
Renata Cozzi

Clarice Patrono

1