Publications by authors named "Carina Argüelles"

12 Publications

  • Page 1 of 1

Assessment of genetic variability in captive capuchin monkeys (Primates: Cebidae).

Sci Rep 2021 Mar 31;11(1):7306. Epub 2021 Mar 31.

Grupo de Investigación en Biología Evolutiva (GIBE), Departamento de Ecología, Genética y Evolución (DEGE), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, C.A.B.A., C1428EGA, Buenos Aires, Argentina.

Capuchin monkeys (genera Cebus and Sapajus) show a wide range distribution, from Honduras to Argentina. The aim of this work was to evaluate the genetic and phenotypic variability of captive specimens putatively belonging to S. cay (SCY) and S. nigritus (SNI) at their southernmost distribution limit. Forty-four individuals held in five captive centers from Argentina were analyzed based on external morphology, karyology and DNA sequences of mitochondrial control region (mtDNA-CR). Three morphotypes associated with their probable geographical origin in SCY and a single morphotype in SNI were found. For SCY we could associate each morphotype with the most frequent karyotype. SNI showed a single phenotype and a homogenous karyotype. Heterochromatin showed geographical patterns within species. A 515-bp mtDNA-CR fragment was sequenced, defining fourteen haplotypes at 59 polymorphic sites. A network constructed with our 14 haplotypes and other 77 from S. apella, S. macrocephalus, S. cay and S. nigritus from bibliography revealed some phylogeographic signals. Our SCY and SNI samples rendered four groups that differed in multiple mutational steps, with SCY being more similar to S. apella than to S. macrocephalus. Also, we identified two genetic divergent SCY groups: samples from NOA and from NEA with high mitochondrial diversity. Our results highlight the relevance of using complementary genetic tools throughout the distribution ranges of SCY and SNI for a better assessment of their diversity.
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http://dx.doi.org/10.1038/s41598-021-86734-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012615PMC
March 2021

Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance.

BMC Res Notes 2020 Oct 7;13(1):470. Epub 2020 Oct 7.

Facultad de Ciencias Exactas, Químicas y Naturales, Departamento de Genética, Universidad Nacional de Misiones (UNaM), Posadas, Misiones, Argentina.

Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris).

Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
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http://dx.doi.org/10.1186/s13104-020-05312-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542955PMC
October 2020

Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis.

Am J Trop Med Hyg 2020 12 3;103(6):2239-2243. Epub 2020 Sep 3.

Instituto de Investigaciones en Microbiología y Parasitología Médica, Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.

Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain ( = 0.0015) or local TgCkAr-11-9 isolate ( = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients ( = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
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http://dx.doi.org/10.4269/ajtmh.20-0124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695064PMC
December 2020

Fine-scale genomic analyses of admixed individuals reveal unrecognized genetic ancestry components in Argentina.

PLoS One 2020 16;15(7):e0233808. Epub 2020 Jul 16.

Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.

Similarly to other populations across the Americas, Argentinean populations trace back their genetic ancestry into African, European and Native American ancestors, reflecting a complex demographic history with multiple migration and admixture events in pre- and post-colonial times. However, little is known about the sub-continental origins of these three main ancestries. We present new high-throughput genotyping data for 87 admixed individuals across Argentina. This data was combined to previously published data for admixed individuals in the region and then compared to different reference panels specifically built to perform population structure analyses at a sub-continental level. Concerning the Native American ancestry, we could identify four Native American components segregating in modern Argentinean populations. Three of them are also found in modern South American populations and are specifically represented in Central Andes, Central Chile/Patagonia, and Subtropical and Tropical Forests geographic areas. The fourth component might be specific to the Central Western region of Argentina, and it is not well represented in any genomic data from the literature. As for the European and African ancestries, we confirmed previous results about origins from Southern Europe, Western and Central Western Africa, and we provide evidences for the presence of Northern European and Eastern African ancestries.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233808PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365470PMC
September 2020

ABSENCE OF PARVOVIRUS SHEDDING IN FECES OF THREATENED CARNIVORES FROM MISIONES, ARGENTINA.

J Zoo Wildl Med 2018 12;49(4):1054-1060

Since its emergence in the 1970s, canine parvovirus (CPV) has spread worldwide and infects a wide variety of mammalian hosts, including domestic and nondomestic carnivores. Today it is one of the most important pathogenic viruses associated with high morbidity and mortality in domestic dogs ( Canis familiaris). In South America, the range of wild hosts has been scarcely studied and the epidemiology of CPV in wildlife is still unclear. In 2011, feces from five wild carnivores (bush dog [ Speothos venaticus] , jaguar [ Panthera onca], puma [ Puma concolor], oncilla [ Leopardus guttulus], and ocelot [ Leopardus pardalis]) were collected in Misiones, Argentina, using a detection dog. Of the 289 feces collected, 209 (72.3%) had sufficient sample remaining to be used in this study and the majority of these were genetically confirmed to individual (81.3%) and sex (78.4%) level. In fact, these samples represent a minimum of 115 individuals (10 jaguars, 13 pumas, 33 ocelots, 38 oncillas, and 21 bush dogs). Through polymerase chain reaction, a 583-bp fragment in the VP2 gene of CPV was amplified in these samples. While no samples showed evidence of infection, this does not exclude the occurrence of CPV in wild carnivores in the area, as intermittent viral shedding could condition the diagnosis of CPV in feces of infected wild mammals. Locally, it is recommended that long-term monitoring of parvovirus be continued in wildlife and expanded to domestic carnivores. Internationally, this study provides a useful contribution to the approach to the sylvatic cycle of parvovirus in wild carnivores.
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http://dx.doi.org/10.1638/2016-0301.1DOI Listing
December 2018

Yerba mate (Ilex paraguariensis, A. St.-Hil.) de novo transcriptome assembly based on tissue specific genomic expression profiles.

BMC Genomics 2018 Dec 7;19(1):891. Epub 2018 Dec 7.

Grupo de Investigación en Genética Aplicada (GIGA), Facultad de Ciencias Exactas Químicas y Naturales, Instituto de Biología Subtropical (IBS UNaM-CONICET), Universidad Nacional de Misiones, Jujuy 1745, CP3300, Posadas, Misiones, Argentina.

Background: The most common infusion in southern Latin-American countries is prepared with dried leaves of Ilex paraguariensis A. St.-Hil., an aboriginal ancestral beverage known for its high polyphenols concentration currently consumed in > 90% of homes in Argentina, in Paraguay and Uruguay. The economy of entire provinces heavily relies on the production, collection and manufacture of Ilex paraguariensis, the fifth plant species with highest antioxidant activity. Polyphenols are associated to relevant health benefits including strong antioxidant properties. Despite its regional relevance and potential biotechnological applications, little is known about functional genomics and genetics underlying phenotypic variation of relevant traits. By generating tissue specific transcriptomic profiles, we aimed to comprehensively annotate genes in the Ilex paraguariensis phenylpropanoid pathway and to evaluate differential expression profiles.

Results: In this study we generated a reliable transcriptome assembly based on a collection of 15 RNA-Seq libraries from different tissues of Ilex paraguariensis. A total of 554 million RNA-Seq reads were assembled into 193,897 transcripts, where 24,612 annotated full-length transcripts had complete ORF. We assessed the transcriptome assembly quality, completeness and accuracy using BUSCO and TransRate; consistency was also evaluated by experimentally validating 11 predicted genes by PCR and sequencing. Functional annotation against KEGG Pathway database identified 1395 unigenes involved in biosynthesis of secondary metabolites, 531 annotated transcripts corresponded to the phenylpropanoid pathway. The top 30 differentially expressed genes among tissue revealed genes involved in photosynthesis and stress response. These significant differences were then validated by qRT-PCR.

Conclusions: Our study is the first to provide data from whole genome gene expression profiles in different Ilex paraguariensis tissues, experimentally validating in-silico predicted genes key to the phenylpropanoid (antioxidant) pathway. Our results provide essential genomic data of potential use in breeding programs for polyphenol content. Further studies are necessary to assess if the observed expression variation in the phenylpropanoid pathway annotated genes is related to variations in leaves' polyphenol content at the population scale. These results set the current reference for Ilex paraguariensis genomic studies and provide a substantial contribution to research and biotechnological applications of phenylpropanoid secondary metabolites.
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http://dx.doi.org/10.1186/s12864-018-5240-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286616PMC
December 2018

Immune Mediators against during Reactivation of Toxoplasmic Retinochoroiditis.

Ocul Immunol Inflamm 2019 6;27(6):949-957. Epub 2018 Aug 6.

Centro de Investigación, Facultad de Ciencias de la Salud, Universidad Católica de las Misiones , Posadas , Argentina.

: The purpose of this article is to analyze possible associations between systemic and ocular cytokine levels and specific clinical ophthalmologic signs from patients with a reactivation of toxoplasmic retinochoroiditis (RTR). : A total of 18 patients with an active RTR episode, 8 patients with inactive scars, and 14 control patients were included in the study. Serum samples and aqueous humor (AH) samples were analyzed for IFN (interferon)-γ, interleukin (IL)-10, and IL-6 levels by ELISA. Inflammation grade, location, and size of the retinochoroidal active lesion, sampling time, and time to resolution were recorded. : A significantly negative correlation between AH and serum levels of IFN-γ was detected ( < 0.05). Patients with an AH IFN-γ/IL-10 ratio lower than 1 were associated with the longest time to resolution and/or severe complications. : Serum IFN-γ levels may be used as a prognostic marker for both time to resolution and the development of possible severe complications during a given RTR episode.
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http://dx.doi.org/10.1080/09273948.2018.1499940DOI Listing
February 2020

Genetic Differentiation of North-East Argentina Populations Based on 30 Binary X Chromosome Markers.

Front Genet 2018 13;9:208. Epub 2018 Jun 13.

Laboratorio de Diversidad Genética, IMBICE, CICPBA-Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata, Argentina.

Alu insertions, INDELs, and SNPs in the X chromosome can be useful not only for revealing relationships among populations but also for identification purposes. We present data of 10 Alu insertions, 5 INDELs, and 15 SNPs of X-chromosome from three Argentinian north-east cities in order to gain insight into the genetic diversity of the X chromosome within this region of the country. Data from 198 unrelated individuals belonging to Posadas, Corrientes, and Eldorado cities were genotyped for Ya5DP62, Yb8DP49, Ya5DP3, Ya5NBC37, Ya5DP77, Ya5NBC491, Ya5DP4, Ya5DP13, Yb8NBC634, and Yb8NBC102 Alu insertions, for MID193, MID1705, MID3754, MID3756 and MID1540 Indels and for rs6639398, rs5986751, rs5964206, rs9781645, rs2209420, rs1299087, rs318173, rs933315, rs1991961, rs4825889, rs1781116, rs1937193, rs1781104, rs149910, and rs652 SNPs. No deviations from Hardy-Weinberg equilibrium were observed for Posadas and Corrientes. However, Eldorado showed significant values, and it was found to have an internal substructuring with two groups of different origin, one showing higher similarity with European countries, and the other with more similarities to Posadas and Corrientes. pairwise genetic distances emerged for some markers among the studied populations and also between our data and those from other countries and continents. Of particular interest, Alu insertions demonstrated the most differences, and could be of use in ancestry studies for these populations, while INDELs and SNPs variation were informative for differentiation within the country.
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http://dx.doi.org/10.3389/fgene.2018.00208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008373PMC
June 2018

Using niche-modelling and species-specific cost analyses to determine a multispecies corridor in a fragmented landscape.

PLoS One 2017 25;12(8):e0183648. Epub 2017 Aug 25.

Facultad de Ciencias Exactas, Químicas y Naturales, Departamento de Genética, Universidad Nacional de Misiones (UNaM), Posadas, Misiones, Argentina.

Misiones, Argentina, contains the largest remaining tract of Upper Paraná Atlantic Forest ecoregion; however, ~50% of native forest is unprotected and located in a mosaic of plantations, agriculture, and pastures. Existing protected areas are becoming increasingly isolated due to ongoing habitat modification. These factors, combined with lower than expected regional carnivore densities, emphasize the need to understand the effect of fragmentation on animal movement and connectivity between protected areas. Using detection dogs and genetic analyses of scat, we collected data on jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis), oncillas (Leopardus tigrinus), and bush dogs (Speothos venaticus) across habitats that varied in vegetation, disturbance, human proximity, and protective status. With MaxEnt we evaluated habitat use, habitat suitability, and potential species richness for the five carnivores across northern-central Misiones, Argentina. Through a multifaceted cost analysis that included unique requirements of each carnivore and varying degrees of overlap among them, we determined the optimal location for primary/secondary corridors that would link the northern-central zones of the Green Corridor in Misiones and identified areas within these corridors needing priority management. A secondary analysis, comparing these multispecies corridors with the jaguar's unique requirements, demonstrated that this multispecies approach balanced the preferences of all five species and effectively captured areas required by this highly restricted and endangered carnivore. We emphasize the potential importance of expanding beyond a single umbrella or focal species when developing biological corridors that aim to capture the varied ecological requirements of coexisting species and ecological processes across the landscape. Detection dogs and genetic analyses of scat allow data on multiple species to be collected efficiently across multiple habitat types independent of the degree of legal protection. These data used with multifocal GIS analyses balance the varying degree of overlap and unique properties among them allowing for comprehensive conservation strategies to be developed relatively rapidly. Our comprehensive approach serves as a model to other regions faced with habitat loss and lack of data. The five carnivores focused on in our study have wide ranges, so the results from this study can be expanded and combined with surrounding countries, with analyses at the species or community level.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0183648PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571917PMC
October 2017

Neotropical Zoonotic Parasites in Bush Dogs (Speothos venaticus) from Upper Paraná Atlantic Forests in Misiones, Argentina.

Vector Borne Zoonotic Dis 2016 10 7;16(10):664-72. Epub 2016 Sep 7.

5 Department of Biology, Washington University , St. Louis, Missouri.

Wildlife remains an important source of zoonotic diseases for the most vulnerable groups of humans, primarily those living in rural areas or coexisting with forest. The Upper Paraná Atlantic forest of Misiones, Argentina is facing ongoing environmental and anthropogenic changes, which affect the local biodiversity, including the bush dog (Speothos venaticus), a small canid considered Near Threatened globally and Endangered locally. This project aimed to expand the knowledge of zoonotic parasites present in the bush dog and the potential implications for human health and conservation medicine. From May to August 2011, a detection dog located 34 scats that were genetically confirmed as bush dog and georeferenced to northern Misiones. Of these 34 scats, 27 had sufficient quantity that allowed processing for zoonotic parasites using morphological (sedimentation and flotation) and antigen (coproantigen technique) analyses. Within these 27 scats, we determined that the parasitic prevalence was 63.0% (n = 17) with 8 (47.1%) having mixed infections with 2-4 parasitic genera. No significant differences (p > 0.05) between sampling areas, sex, and parasite taxa were found. We were able to summarize the predominant nematodes (Ancylostoma caninum, Toxocara canis, and Lagochilascaris spp.), cestodes (Taenia spp. and Spirometra spp.), and apicomplexa (Cystoisospora caninum) found in these bush dogs. With the copro-ELISA technique, 14.8% (n = 4) of the samples were positive for Echinococcus spp. This study represents the first comprehensive study about parasitic fauna with zoonotic potential in the free-ranging bush dog. This information combined with the innovative set of techniques used to collect the samples constitute a valuable contribution that can be used in control programs, surveillance of zoonotic diseases, and wildlife conservation, both regionally and across the bush dog's broad distribution.
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http://dx.doi.org/10.1089/vbz.2015.1929DOI Listing
October 2016

Using detection dogs and genetic analyses of scat to expand knowledge and assist felid conservation in Misiones, Argentina.

Integr Zool 2014 Nov;9(5):623-39

Department of Biology, University of Missouri, St. Louis, USA; WildCare Institute at the Saint Louis Zoo, St. Louis, USA.

Many carnivores require large ranges to meet their ecological and energetic needs; however, anthropogenic changes threaten species and their habitats. Camera traps have been used to effectively collect data on carnivores in a variety of habitat types; however, a single survey effort is typically limited to species that have similar body size, habitat use and movement patterns, and individual identification of animals is not always possible. We evaluated whether scat detection dogs could effectively survey for 4 wide-ranging felids that vary in these characteristics: jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis) and oncillas (Leopardus tigrinus). From June to October 2009 and May to August 2011, a detection dog-handler team detected 588 scats, from which 176 unique genotypes were detected. We assigned sex to 84.7% of the genotyped scats and identified 55 individuals multiple times. The effectiveness of these noninvasive techniques (detection dogs and genetic analyses of scat) not only opens the door for additional studies in areas that were previously difficult or impossible with standard survey techniques, but also provides conservationists with a set of tools that overcome some of the limitations associated with the use of camera traps alone.
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http://dx.doi.org/10.1111/1749-4877.12113DOI Listing
November 2014

Genetic analysis of six communities of Mbyá-Guaraní inhabiting northeastern Argentina by means of nuclear and mitochondrial polymorphic markers.

Hum Biol 2010 Aug;82(4):433-56

Servicio de Huellas Digitales Genéticas y Cátedra de Genética y Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Autosomal STRs, Y-chromosome markers, and mitochondrial DNA sequences were investigated in six Mbyá-Guaraní villages (Fortín M'Bororé, Yryapu, Tabay, Kaaguy Poty, Jejy, and Yaboti), all of them settled within the province of Misiones, northeastern Argentina. One hundred twenty-one unrelated individuals were analyzed. The study involved typing fifteen autosomal STRs, nine Y-chromosome STRs, and four biallele loci in the nonrecombinant region of the Y chromosome, sequencing the mtDNA of hypervariable regions I and II, and detecting the 9-bp ins/del in region V of mtDNA. All autosomal STRs were in Hardy-Weinberg equilibrium. The four major native American mtDNA haplogroups were represented in the sample. Haplogroups A2 and D1 exhibited the highest frequencies (40.5% and 36.0%, respectively), and haplogroups B2 and C1 appeared to be less frequent (17.5% and 6.0%, respectively). The native American haplogroup Q1a3a was observed in a relevant proportion (88.8%). In addition, a nine-STR Y-chromosome haplo-type (DYS19*13, DYS389I*14, DYS389II*31, DYS390*24, DYS391*11, DYS392*14, DYS393*11, DYS385A*14, DYS385B*16) exhibited a frequency of more than 36%. Our results indicate that the analyzed Argentinean Guaraní individuals are genetically more closely related to Guaraní from Brazil [genetic distance (Δµ)(2) = 0.48] than to other related tribes that are geographically closer. Statistical approaches based on autosomal data do not support the hypothesis of genetic drift previously proposed; however, this apparent discrepancy might be due to the lack of sensitivity of the autosomal markers used here.
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http://dx.doi.org/10.3378/027.082.0406DOI Listing
August 2010