Publications by authors named "Can Cui"

200 Publications

Stabilizing Lithium Metal Anode Enabled by a Natural Polymer Layer for Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2021 Jun 8. Epub 2021 Jun 8.

MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

The lithium-sulfur (Li-S) battery with a high theoretical energy density (2560 Wh kg) is one of the most promising candidates in next-generation energy storage systems. However, its practical application is impeded by the shuttle effect of lithium polysulfides, huge volume expansion, and overgrowth dendrite of lithium. Herein, we propose an artificial conformal agar polymer coating on a lithium anode (marked as A-Li). The functional layer facilitating the formation of a compact interphase on the lithium anode can effectively accommodate expansive volume and restrain the growth of dendritic lithium. The Li/Li symmetric cell with A-Li delivers stable plating/stripping cycling over 300 h at a high current density of 3.0 mA cm and a high fixed areal capacity of 3.0 mAh cm. The cycle life of Li-Cu cells with A-Li is twice longer than that of pristine cells, and the Li-S batteries equipped with A-Li anodes also deliver an enhanced specific capacity and high Coulombic efficiencies. This work provides a pathway to protect metal Li anodes and contributes to the development of high-performance Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c06289DOI Listing
June 2021

Risk factors for rapid axial length elongation with low concentration atropine for myopia control.

Sci Rep 2021 Jun 3;11(1):11729. Epub 2021 Jun 3.

The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe road, Zhengzhou, 450000, China.

Three hundred and twenty-eight myopic children, randomized to use either 0.01% (N = 166) or 0.02% (N = 162) atropine were enrolled in this study. Gender, age, body mass index(BMI), parental myopia status, atropine concentration used, pupil diameter, amplitude of accommodation, spherical equivalent refractive error (SER), anterior chamber depth (ACD) and axial length (AL) were collected at baseline and 1 year after using atropine. Rapid AL elongation was defined as > 0.36 mm growth per year. Univariate analyses showed that children with rapid AL elongation tend to be younger, have a smaller BMI, use of 0.01% atropine, narrow ACD, lower SER, shorter AL, smaller change in pupil diameter between 1 year and baseline (all P < 0.05). Multivariate logistic regression analyses confirmed that rapid AL elongation was associated with children that were younger at baseline (P < 0.0001), use of 0.01% atropine (P = 0.04), a shorter baseline AL (P = 0.03) and a smaller change in pupil diameter between 1 year and baseline (P = 0.04). Younger children with shorter AL at baseline, less change in their pupil diameter with atropine treatment and using the lower of the two atropine concentrations may undergo rapid AL elongation over a 12 months myopia control treatment period.
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http://dx.doi.org/10.1038/s41598-021-88719-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175344PMC
June 2021

Magnesium supplementation alleviates corticosteroid-associated muscle atrophy in rats.

Eur J Nutr 2021 May 30. Epub 2021 May 30.

Musculoskeletal Research Laboratory of Department of Orthopaedics and Traumatology and Innovative Orthopaedic Biomaterial and Drug Translational Research Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Rm74026, 5/F, Clinical Science Building, Prince of Wales Hospital, Shatin, Hong Kong SAR, People's Republic of China.

Purpose: Corticosteroid (CS) therapy for infectious and rheumatological diseases showed to decrease serum magnesium (Mg) level and induce muscle atrophy in patients. The present study investigated the effects of Mg supplementation on preventing CS-induced muscle atrophy in an animal model, which provided experimental data for potential clinical translation.

Methods: Twelve 24-week-old male Sprague-Dawley rats were treated with lipopolysaccharide (LPS) and CS methylprednisolone (MPS) to induce muscle atrophy, with half of the rats also given daily 50 mg/kg Mg oral supplementation. Additional six rats without LPS + CS treatments were used as normal controls. After treatment for 6 weeks, serum was collected for Mg quantification, animal dual-energy X-ray absorptiometry (DXA) was performed for tissue composition, and the extensor digitorum longus (EDL) was collected for muscle functional test and histology including muscle fiber size, intramuscular fat infiltration and fiber typing. In vitro myotube atrophy model was used to study the in vitro effect associated with in vivo muscle atrophy.

Results: LPS + CS treatments induced hypomagnesemia while the serum Mg level was in normal range after Mg supplementation. DXA showed 53.0% lower fat percent and 29.7% higher lean mass in LPS + CS + Mg group when compared to LPS + CS group. Muscle functional test showed 22.2% higher specific twitch force and 40.3% higher specific tetanic force in LPS + CS + Mg group when compared to LPS + CS group. Histological analysis showed 4.1% higher proportion of muscle fibers area to total area and 63.6% lower intramuscular fat infiltration in EDL sections in LPS + CS + Mg group when compared to LPS + CS group. LPS + CS + Mg group had 33.0% higher area proportion and 29.4% higher cross-sectional area (CSA) of type IIb muscle fiber. Myoblast culture results showed that Mg supplementation group had larger myotube diameter. The mRNA expressions of the muscle atrophy marker genes MuRF1 and MAFbx were lower in Mg supplementation group both in vitro and in vivo.

Conclusion: The current study demonstrated that Mg supplementation successfully alleviated CS-associated muscle atrophy in rats at both functional and morphology levels, indicating a translational potential for patients undergoing CS therapy. This study provided the evidence for the first time that Mg supplementation could prevent muscle atrophy-an adverse effect of CS therapy, currently also adopted for treating coronavirus disease 2019 (COVID-19).
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http://dx.doi.org/10.1007/s00394-021-02598-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164831PMC
May 2021

Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR‑145‑3p/NFATc2/NF‑κB axis.

Int J Mol Med 2021 Jul 26;48(1). Epub 2021 May 26.

Department of Anesthesiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China.

Propofol is a commonly used intravenous anesthetic. The aim of the study was to examine the mechanism of propofol in traumatic brain injury (TBI) by regulating interleukin (IL)‑17 activity and maintaining the Th17/Treg balance. A rat model with moderate TBI was established using the weight‑drop method. Rats with TBI were regularly injected with propofol and their brain injuries were monitored. The peripheral blood of rats was collected to measure the Th17/Treg ratio. MicroRNA (miR)‑145‑3p expression was detected in the brain tissues of rats and antagomiR‑145‑3p was injected into the lateral ventricles of their brains to verify the effect of miR‑145‑3p on brain injury. The downstream target of miR‑145‑3p was predicted. The targeting relationship between miR‑145‑3p and nuclear factor of activated T cells c2 (NFATc2) was confirmed. NFATC2 expression and phosphorylation of NF‑κB pathway‑related proteins were measured. Propofol alleviated brain injury in rats with TBI and maintained the Th17/Treg balance. Propofol upregulated miR‑145‑3p expression in rat brains, while the inhibition of miR‑145‑3p reversed the effect of propofol on brain injury. A binding relationship was observed between miR‑145‑3p and NFATc2. Furthermore, propofol decreased the phosphorylation of p65 and IκBα, and inhibited activation of the NF‑κB pathway in the brains of rats with TBI. In conclusion, propofol maintained Th17/Treg balance and reduced inflammation in the rats with TBI via the miR‑145‑3p/NFATc2/NF‑κB axis.
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http://dx.doi.org/10.3892/ijmm.2021.4968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148094PMC
July 2021

Coping Styles for Mediating the Effect of Resilience on Depression Among Medical Students in Web-Based Classes During the COVID-19 Pandemic: Cross-sectional Questionnaire Study.

J Med Internet Res 2021 06 7;23(6):e25259. Epub 2021 Jun 7.

Department of Social Medicine, College of Health Management, China Medical University, Shenyang, Liaoning Province, China.

Background: Due to strict, nationwide, comprehensive COVID-19 protective measures, including home quarantine, all Chinese medical students began taking web-based classes beginning in the spring semester of 2020. Home quarantine, web-based classes, and the stress surrounding the COVID-19 pandemic may have triggered an increased incidence of mental health problems among medical students. Although there have been increasing amounts of literature on depression among medical students, studies focusing on positive psychological resources, such as resilience during the COVID-19 pandemic, still need to be expanded.

Objective: This study aims to assess depression among medical students who are taking web-based classes during the COVID-19 pandemic and to investigate the role of coping styles as mediators between resilience and depression.

Methods: A cross-sectional study of 666 medical students involving stratified sampling in Shenyang, Liaoning Province, China, was completed between March 20 and April 10, 2020. The participants responded to a self-administered, smartphone-based questionnaire, which included the Patient Health Questionnaire-9, Simplified Coping Style Questionnaire, and Ego Resilience 89 Scale. Hierarchical linear regression and structural equation modeling were used in this study.

Results: The prevalence of depression among the participants was 9.6% (64/666) in this study. The regression analysis revealed that grade (the year in which the medical student was in training) (P=.013), how well students adapted to web-based classes (P<.001), their levels of resilience (P=.04), and their coping styles were independent predictors for depression (P<.001). Resilience and positive coping styles were negatively related to depression (resilience: P=.04; positive coping styles: P<.001), and negative coping styles were positively related to depression (P<.001). The structural equation modeling analysis showed that the effect of resilience on depression was partially mediated by coping styles (P=.007).

Conclusions: In this study, it was found that the prevalence of depression was slightly low and coping styles mediated the association between resilience and depression among medical students during the COVID-19 pandemic. These findings have significant implications for future studies. Future studies and interventions should aim to improve resilience and promote positive coping styles.
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http://dx.doi.org/10.2196/25259DOI Listing
June 2021

Thermal conductivity and mechanical performance of hexagonal boron nitride nanosheets-based epoxy adhesives.

Nanotechnology 2021 Jun 11;32(35). Epub 2021 Jun 11.

College of Aerospace Engineering, Shenyang Aerospace University, Shenyang 110136, People's Republic of China.

Thermosets possess diverse physical and chemical properties and thus they are widely used in various applications such as electronic packaging, construction, and automotive industries. However, their poor thermal conductivity and weak mechanical performance jeopardize their continual spread in modern industry. In this study, boron nitride nanosheets (BNNSs) were employed to promote both mechanical and thermal properties of epoxy nanocomposites. BNNSs and their epoxy nanocomposites were fabricated usingsolvent ultrasonication andpolymerization, respectively. Thermal conductivity was enhanced by 153% increment in epoxy/BNNS nanocomposite at 7 wt% in comparison with neat epoxy. In parallel, Young's modulus, lap shear strength, fracture toughness () and energy release rate () increased by 69%, 31%, 122% and 118%, respectively at 1 wt% BNNSs. Moreover, fatigue life and strength of lap shear joints were significantly improved upon adding BNNSs. A numerical model of the single lap shear joint was developed to validate the accuracy of the material constants obtained. Epoxy/BNNS nanocomposites exhibited an outstanding mechanical performance as well as high thermal conductivity giving them merits to widen their applications in electronic and automotive industry.
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http://dx.doi.org/10.1088/1361-6528/ac0470DOI Listing
June 2021

Variational hysteresis and photoresponse behavior of MAPb(= I, Br, Cl) perovskite single crystals.

J Phys Condens Matter 2021 Jun 3;33(28). Epub 2021 Jun 3.

Key Laboratory of Optical Field Manipulation of Zhejiang Province, Department of Physics, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.

High-quality MAPb(= I, Br, Cl) single crystals with a desirable size were grown through an inverse temperature crystallization method. Systematically measurements of current-voltage (-) hysteresis show that the hysteresis is strongly dependent on the measuring protocol, including scan rate and light illumination condition, which reveals the competition of three main factors that influence the charge dynamics in different regimes, defect trap, MAdipoles rotation, and ion migration. In the dark, defect trapping is the dominant charge transport dynamics at low bias in the MAPbI, while the MAdipole rotation is significant in MAPbBr, and ion migration occurs in MAPbCl. However, as bias increases, MAdipole rotation plays a crucial role in the conductivity either in the dark or under light illumination. The time-dependent photoresponse exhibits different tendencies under various biases. The slow rising dynamics of photoresponse in MAPbis attributed to the slow rotation of MAdipoles, while an immediate overshoot followed by a decay suggests significant ion migration contribution at high external bias. The results serve as comprehensive experimental support to understand the hysteresis behaviors and slow photoresponse in MAPb, particularly in MAPbCl, and provide a guide for future work in MAPbbased optoelectronic devices.
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http://dx.doi.org/10.1088/1361-648X/abff92DOI Listing
June 2021

Capture or suppression? Attentional allocation upon reward and loss-associated nonsalient distractors are supported by distinct neural mechanisms: An EEG study.

Neuropsychologia 2021 Jul 3;157:107879. Epub 2021 May 3.

School of Psychology, Northeast Normal University, Changchun, China.

Previous studies suggest that a reward-associated salient distractor can induce bottom-up attentional capture. Hitherto, the neurophysiological mechanisms underlying attentional allocation upon reward/loss associated nonsalient stimulus remain hardly investigated. The present study built the association between nonsalient stimuli and value, and tested it with a decision-making task. Consequently, we examined whether and how reward/loss-associated nonsalient stimuli (as distractors) influenced attentional allocation in a rapid serial visual presentation task. Behavioral analysis showed a significantly faster recognition of target in the loss condition compared to performance in the neutral/reward conditions. Electrophysiological results showed that reward-associated distractors induced a significant Pd component, while loss-associated distractors induced a significantly higher theta oscillation. These results demonstrated that subjects could proactively suppress reward-associated distractors. More importantly, we showed that attentional allocation upon reward/loss-associated nonsalient distractors is supported by distinct neural mechanisms.
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http://dx.doi.org/10.1016/j.neuropsychologia.2021.107879DOI Listing
July 2021

Factors Influencing Anxiety Among WeChat Users During the Early Stages of the COVID-19 Pandemic in Mainland China: Cross-sectional Survey Study.

J Med Internet Res 2021 05 17;23(5):e24412. Epub 2021 May 17.

Department of Social Medicine, School of Public Health, China Medical University, Shenyang, China.

Background: The rapid outbreak of COVID-19 around the world has adversely affected the mental health of the public. The prevalence of anxiety among the public has increased dramatically during the COVID-19 pandemic. However, there are few studies evaluating the effects of positive psychological responses and information-seeking behaviors on anxiety experienced among social media users during the COVID-19 pandemic.

Objective: This study evaluated the prevalence of anxiety and its associated factors among WeChat users in mainland China during the early stages of the COVID-19 pandemic.

Methods: From February 10 to February 24, 2020, a nationwide, web-based cross-sectional survey study was carried out using convenience sampling. Participants' levels of anxiety, positive psychological responses, and information-seeking behaviors were assessed. The survey was distributed among WeChat users via the WeChat smartphone platform. Chi-square tests and multivariable logistic regression analyses were performed to examine the factors associated with anxiety.

Results: This study found that the prevalence of anxiety (Generalized Anxiety Disorder 7-item [GAD-7] scale score ≥7) among WeChat users in China was 17.96% (446/2483) during the early stages of the COVID-19 pandemic. Results of multivariable logistic regression analysis showed that information-seeking behaviors such as cannot stop searching for information on COVID-19, being concerned about the COVID-19 pandemic, and spending more than 1 hour per day consuming information about the pandemic were found to be associated with increased levels of anxiety. Additionally, participants who chose social media and commercial media as the primary sources to obtain information about the COVID-19 pandemic were found more likely to report anxiety. Conversely, participants who were confident or rational about the COVID-19 pandemic were less likely to report anxiety.

Conclusions: This study found that positive psychological responses and information-seeking behaviors were closely associated with anxiety among WeChat users during the COVID-19 pandemic in China. It might be paramount to enhance mental well-being by helping people respond to the COVID-19 pandemic more rationally and positively in order to decrease symptoms of anxiety.
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http://dx.doi.org/10.2196/24412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130820PMC
May 2021

Association of HMOX-1 with sporadic Alzheimer's disease in southern Han Chinese.

Eur J Neurol 2021 Apr 17. Epub 2021 Apr 17.

Department of Neurology, East Hospital, Tongji University School of Medicine, Shanghai, China.

Study Objectives: To discover the associations between HMOX-1 and Alzheimer's disease (AD).

Methods: 500 AD patients and 500 healthy controls were recruited in this study. Polymer chain reaction (PCR) was used.

Results: The dominant model and the recessive model of HMOX-1 rs2071746 were shown statistically significant between AD patients and control after adjustment of age, gender and education (Dominant model: p value: 0.047, OR: 1.34, 95% CI: 1.00 - 1.78, adjusted; recessive model: p value: 0.049, OR: 1.34, 95% CI: 1.00 - 1.80, adjusted). There is also a trend of association between dominant model and LOAD after adjustment of age, gender and education (Dominant model: p value: 0.084, OR: 1.37, 95% CI: 0.96 - 1.95, adjusted).

Conclusions: We found the association of the dominant model and the recessive model of HMOX1 rs2071746 with AD.
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http://dx.doi.org/10.1111/ene.14870DOI Listing
April 2021

The mnemonic effects of insight on false memory in the DRM paradigm.

Psychol Res 2021 Apr 12. Epub 2021 Apr 12.

College of Education, Hebei University, No. 180 of Wusi East Road, Baoding, China.

Insight accompanied by an 'aha!' experience has a mnemonic effect. Previous studies of insight have often focused on the mnemonic effect of insight on veridical memories, while the effect of insight on false memories is not known. More understanding of the mnemonic effect of insight on false memories could have implications for the mechanism of insightful mnemonic effects. The present research examined whether insight has a mnemonic effect on false memories. Participants were asked to perform Chinese verbal compound remote associate (CRA) tasks and then complete the Deese-Roediger-McDermott (DRM) task, the critical lure of which was also the solution to the Chinese CRA problem. Compared to non-insight, insight was associated with a lower critical lures rate in Experiment 1 and with lower critical lures and unrelated words rates in Experiment 2 when the presentation of DRM list words was random. Giving a warning before DRM tasks could reduce the critical lures rate of non-insight but had little effect on insightful solutions in Experiment 3. These findings indicate that insight can reduce false memories.
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http://dx.doi.org/10.1007/s00426-021-01513-6DOI Listing
April 2021

Thyrotroph embryonic factor polymorphism predicts faster progression of Parkinson's disease in a longitudinal study.

J Integr Neurosci 2021 Mar;20(1):95-101

Department of Neurology, Affiliated Brain Hospital of Nanjing Medical University, No. 264 Guangzhou Road, Nanjing, 210029 Jiangsu Province, P. R. China.

The thyrotroph embryonic factor gene is a circadian clock-controlled gene. The rs738499 polymorphism of this gene has been suggested to be associated with depression and sleep disturbance in Parkinson's disease in previous cross-sectional studies. We aimed to investigate whether this single nucleotide polymorphism is associated with the progression rates of various motor and non-motor symptoms in patients with Parkinson's disease. We recruited 186 patients with Parkinson's disease for a longitudinal study. Motor and non-motor symptoms were assessed at baseline and follow-up, and 170 Parkinson's disease patients completed the clinical evaluation twice with an average follow-up period of 3.3 ± 1.1 years. A stepwise linear regression model was used to validate factors associated with Parkinson's disease symptoms' annual progression rates. Faster annual worsening rates of sleep quality and Hoehn-Yahr stage were found in carriers with the homozygous dominant (TT). After adjustment for related clinical factors, the rs738499 polymorphism showed a contribution of 3.1% to the annual decline rate on the Parkinson's Disease Sleep Scale score and a contribution of 5.5% to the annual increase rate of the Hoehn-Yahr stage. Additionally, anxiety and axial symptoms predicted the progression of sleep disturbances and motor staging. The TT genotype of rs738499 might be a potential predictor of rapid deterioration in sleep quality and Hoehn-Yahr stage in patients with Parkinson's disease and may advance the understanding of the genetic contributions to Parkinson's disease.
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http://dx.doi.org/10.31083/j.jin.2021.01.366DOI Listing
March 2021

Hydrographic data inspection and disaster monitoring using shipborne radar small range images with electronic navigation chart.

PeerJ Comput Sci 2020 14;6:e290. Epub 2020 Sep 14.

Maritime College, Guangdong Ocean University, Zhanjiang, Guangdong, China.

Shipborne radars cannot only enable navigation and collision avoidance but also play an important role in the fields of hydrographic data inspection and disaster monitoring. In this paper, target extraction methods for oil films, ships and coastlines from original shipborne radar images are proposed. First, the shipborne radar video images are acquired by a signal acquisition card. Second, based on remote sensing image processing technology, the radar images are preprocessed, and the contours of the targets are extracted. Then, the targets identified in the radar images are integrated into an electronic navigation chart (ENC) by a geographic information system. The experiments show that the proposed target segmentation methods of shipborne radar images are effective. Using the geometric feature information of the targets identified in the shipborne radar images, information matching between radar images and ENC can be realized for hydrographic data inspection and disaster monitoring.
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http://dx.doi.org/10.7717/peerj-cs.290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924651PMC
September 2020

Plectin regulates Wnt signaling mediated-skeletal muscle development by interacting with Dishevelled-2 and antagonizing autophagy.

Gene 2021 May 9;783:145562. Epub 2021 Mar 9.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:

Skeletal muscle is the most abundant tissue in the human and animal body, loss of its function can lead to muscle aging and various myogenic diseases. The skeletal muscle development is a complex and tightly regulated process, which is driven by a variety of many factors, signaling pathways and regulatory mechanisms. Plectin (Plec), a cytolinker protein, is ubiquitously expressed in various tissues such as skin, muscle, plasma membrane, and most types of cells. Although known isoforms of Plec is well-characterized in muscle dystrophy, very little is known on the function of Plec in the skeletal muscle development. Here, we found that Plec plays a vital role in promoting C2C12 myoblasts differentiation and proliferation, but inhibits their apoptosis. Also, Plec regulates the expression of atrophy-related genes (atrogin-1 and muRF-1) to rescue muscle atrophy. Furthermore, we have demonstrated that Plec binds to Dishevelled-2 (Dvl-2) and forms a protein complex, which is then activate the canonical Wnt signaling. We also observed that Plec resists ubiquitination by stabilizing Dvl-2 and reduces the level of LC3-labeled Dvl-2 and antagonizes the autophagy system. In conclusion, our findings suggest that Plec regulates canonical Wnt signaling mediated skeletal development by stabilizing Dvl-2 and downregulating the cellular autophagic degradation system.
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http://dx.doi.org/10.1016/j.gene.2021.145562DOI Listing
May 2021

The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.

Cell Metab 2021 Mar;33(3):565-580.e7

Shanghai Diabetes Institute, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 200233 Shanghai, China. Electronic address:

Stimulation of adipose tissue thermogenesis is regarded as a promising avenue in the treatment of obesity. However, pharmacologic engagement of this process has proven difficult. Using the Connectivity Map (CMap) approach, we identified the phytochemical hyperforin (HPF) as an anti-obesity agent. We found that HPF efficiently promoted thermogenesis by stimulating AMPK and PGC-1α via a Ucp1-dependent pathway. Using LiP-SMap (limited proteolysis-mass spectrometry) combined with a microscale thermophoresis assay and molecular docking analysis, we confirmed dihydrolipoamide S-acetyltransferase (Dlat) as a direct molecular target of HPF. Ablation of Dlat significantly attenuated HPF-mediated adipose tissue browning both in vitro and in vivo. Furthermore, genome-wide association study analysis indicated that a variation in DLAT is significantly associated with obesity in humans. These findings suggest that HPF is a promising lead compound in the pursuit of a pharmacological approach to promote energy expenditure in the treatment of obesity.
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http://dx.doi.org/10.1016/j.cmet.2021.02.007DOI Listing
March 2021

Deletion of FGF9 in GABAergic neurons causes epilepsy.

Cell Death Dis 2021 02 19;12(2):196. Epub 2021 Feb 19.

Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China.

Fibroblast growth factor 9 (FGF9) has long been assumed to modulate multiple biological processes, yet very little is known about the impact of FGF9 on neurodevelopment. Herein, we found that loss of Fgf9 in olig1 progenitor cells induced epilepsy in mice, with pathological changes in the cortex. Then depleting Fgf9 in different neural populations revealed that epilepsy was associated with GABAergic neurons. Fgf9 CKO in GABAergic neuron (CKO) mice exhibited not only the most severe seizures, but also the most severe growth retardation and highest mortality. Fgf9 deletion in CKO mice caused neuronal apoptosis and decreased GABA expression, leading to a GABA/Glu imbalance and epilepsy. The adenylate cyclase/cyclic AMP and ERK signaling pathways were activated in this process. Recombinant FGF9 proteoliposomes could significantly decrease the number of seizures. Furthermore, the decrease of FGF9 was commonly observed in serum of epileptic patients, especially those with focal seizures. Thus, FGF9 plays essential roles in GABAergic neuron survival and epilepsy pathology, which could serve as a new target for the treatment of epilepsy.
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http://dx.doi.org/10.1038/s41419-021-03478-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896082PMC
February 2021

Creatinine and C-reactive protein in amyotrophic lateral sclerosis, multiple sclerosis and Parkinson's disease.

Brain Commun 2020 18;2(2):fcaa152. Epub 2020 Sep 18.

Unit of Integrative Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Serum creatinine and C-reactive protein have been proposed as potential biomarkers for neurodegenerative diseases, including amyotrophic lateral sclerosis, multiple sclerosis and Parkinson's disease. However, longitudinal studies investigating temporal patterns of these biomarkers, including the phase before diagnosis, are rare. We performed a case-control study including all newly diagnosed patients with amyotrophic lateral sclerosis ( = 525), multiple sclerosis ( = 1815) or Parkinson's disease ( = 3797) during 2006-2013 in Stockholm, Sweden, who participated in the Stockholm CREAtinine Measurements (SCREAM) project. For each case, we randomly selected up to five controls from SCREAM that were individually matched to the case by age, sex and county of residence ( = 2625 for amyotrophic lateral sclerosis,  = 9063 for multiple sclerosis and 18 960 for Parkinson's disease). We collected for both the cases and the controls testing results of serum creatinine and C-reactive protein performed by healthcare providers in Stockholm during the study period. Median levels of creatinine and C-reactive protein were visualized using locally weighted smoothing curves among cases and controls. A linear mixed model was also applied to explore temporal changes within an individual. Compared to controls, patients with amyotrophic lateral sclerosis had lower levels of creatinine from 2 years before diagnosis onwards. In contrast, patients with amyotrophic lateral sclerosis had lower levels of C-reactive protein before diagnosis but higher levels after diagnosis, compared to controls. Focusing the 2 years before to 2 years after diagnosis, patients with amyotrophic lateral sclerosis displayed statistically significantly decreasing level of creatinine from 1 year before diagnosis until 2 years after diagnosis, whereas increasing level of C-reactive protein from diagnosis until 2 years after diagnosis. There were no similar patterns noted among patients with multiple sclerosis or Parkinson's disease, or the controls of the three patient groups. Patients with amyotrophic lateral sclerosis display distinct temporal patterns of creatinine and C-reactive protein before and after diagnosis, compared to amyotrophic lateral sclerosis-free controls or patients with multiple sclerosis and Parkinson's disease.
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http://dx.doi.org/10.1093/braincomms/fcaa152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850290PMC
September 2020

New insight into the rapid growth of the Mikania micrantha stem based on DIA proteomic and RNA-Seq analysis.

J Proteomics 2021 03 1;236:104126. Epub 2021 Feb 1.

College of Life Sciences, South China Agricultural University, Wushan 483, Guangzhou 510642, China. Electronic address:

Mikania micrantha is one of the world's most invasive plants, which causes severe damage to natural ecosystems and agroforestry systems due to its rapid stem growth. This work investigated the proteomic and transcriptomic profiles of M. micrantha in different stem tissues (pre-internode, post-internode, and internode), as well as in adventitious roots and primary roots with the final goal of elucidating differentially expressed genes and proteins responsible for the rapid growth of stem. The objective was approached by using DIA-based proteomic and RNA-Seq technologies. More than seven giga-transcriptome clean reads were sequenced, and 5196 protein species were identified. Differentially expressed genes identified in all stem tissues were significantly enriched in photosynthesis and carbon fixation, suggesting that the stem possesses a strong photosynthetic capacity in order to maintain the energy supply for this species. Analysis of differentially expressed proteins showed that proteins related to photosystem I/II and the cytochrome b6/f complex, such as D1, D2, and cp43, were also highly accumulated in the adventitious roots, corroborating the transcriptome analysis results. These results provided basic proteomic and transcriptional expression information about the M. micrantha stem and adventitious root, thereby improving our understanding of the molecular mechanism underlying rapid growth in this species. SIGNIFICANCE: This is the first study to investigate the proteomic and transcriptomic profiles of Mikania micrantha, a highly invasive plant, in different stem tissues (pre-internode, post-internode, and internode), as well as in adventitious and primary roots, using the latest DIA-based (data-independent acquisition mode) proteomic and RNA-Seq technologies. A comprehensive study was carried out, and differentially expressed genes and differentially expressed proteins identified in the pre-internode, post-internode, and internode tissues were significantly enriched during photosynthesis and carbon fixation, suggesting that the M. micrantha stem possesses a strong photosynthetic capacity that allows the plant to maintain a high energy supply. Enriched plant hormone signal transduction pathway analysis revealed an interaction between auxin and other phytohormones involved in adventitious root development. The study provided basic data on the molecular mechanism of M. micrantha vegetative propagation and the rapid growth of its stem. The novel scientific content of this study successfully builds upon the limited information currently available on the subject, therefore warranting publication.
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http://dx.doi.org/10.1016/j.jprot.2021.104126DOI Listing
March 2021

Cost-effectiveness analysis of dabigatran, rivaroxaban and warfarin in the prevention of stroke in patients with atrial fibrillation in China.

BMC Health Serv Res 2021 Jan 28;21(1):96. Epub 2021 Jan 28.

Department of Pharmacy, Beijing Friendship Hospital, Capital Medical University, 100050, Beijing, China.

Background And Objective: To evaluate the cost-effectiveness of new anticoagulants and warfarin in the prevention of stroke in Chinese patients with atrial fibrillation (AF).

Methods: The Markov model was constructed to compare patients' quality-adjusted life-years (QALYs) using drug cost, the cost of the examination after taking a drug, and the incremental cost of other treatments. Both dabigatran (110 and 150 mg, twice a day) and rivaroxaban (20 mg, once a day) were compared with warfarin (3-6 mg, once a day). Willingness to pay, three times the 2018 China GDP per capita (9481.88 $), was the cost-effect threshold in our study.

Results: The total cost were was 5317.31$, 29673.33$, 23615.49$, and 34324.91$ for warfarin, rivaroxaban, dabigatran 110 mg bid, and dabigatran 150 mg bid, respectively. The QALYs for each of the four interventions were 11.07 years, 15.46 years, 12.4 years, and 15 years, respectively. The cost-effectiveness analysis of the three new oral anticoagulants and warfarin showed that the incremental cost-effectiveness ratio (ICER) was 5548.07$/QALY when rivaroxaban was compared with warfarin. Rivaroxaban was the most cost-effective choice and warfarin was the least.

Conclusions: In Chinese patients with AF, although warfarin is cheaper, rivaroxaban has a better cost-effectiveness advantage from an economic point of view.
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http://dx.doi.org/10.1186/s12913-021-06084-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841891PMC
January 2021

Antisolvent engineering on low-temperature processed CsPbI inorganic perovskites for improved performances of solar cells.

Nanotechnology 2021 Apr;32(18):185402

Key Laboratory of Optical Field Manipulation of Zhejiang Province, Department of Physics, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.

CsPbI inorganic perovskites with ideal bandgap and much enhanced thermal stability compared with organic-inorganic hybrid perovskites, have attracted much interest in the field of solar cells. The performances of solar cells highly depend on the quality of perovskite films, yet the research on fabrication methods of inorganic perovskites is far below that of organic-inorganic hybrid counterparts. Antisolvent engineering is a widely used method in controlling the morphology and crystallinity of organic-inorganic hybrid perovskites. Its effect varies with parameters such as the physicochemical properties of antisolvents and the compositions of perovskite precursors. Specially, there lacks a comprehensive study comparing different antisolvents used in low-temperature processed CsPbI from dimethylammonium-based precursors. In this work, we used three different antisolvents to control the growth of CsPbI films in a low-temperature (<200 °C) processed procedure and systematically compared the properties of resultant films. The green antisolvent ethyl acetate (EA) engineered CsPbI films exhibit improved morphology and crystallinity as well as reduced defects, compared with the counterparts processed without antisolvent or those with widely employed toxic antisolvents toluene and chlorobenzene. The EA antisolvent engineering results in efficient CsPbI perovskite solar cells with a champion power conversion efficiency of 8.8%. Our work thus provides a green and viable way to prepare high quality CsPbI perovskite films for optoelectronic applications.
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http://dx.doi.org/10.1088/1361-6528/abde03DOI Listing
April 2021

Prevalence and dynamic characteristics of generalized joint hypermobility in college students.

Gait Posture 2021 Feb 14;84:254-259. Epub 2020 Dec 14.

Department of Orthopaedics, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510000, Guangdong, China. Electronic address:

Background: Generalized joint hypermobility (GJH) is a common, but often ignored, condition characterized by general joint laxity, which is reported to increase one's risk of anterior cruciate ligament (ACL) injury and osteoarthritis. Nevertheless, it is not clearly learned in the prevalence and dynamic characteristics in college students.

Research Question: Is the active motion stability in the six-degree-of-freedom (6DOF) kinematics of the knee joint of people with GJH poorer than that of others?

Methods: This is a cross-sectional study. A population of 489 college students was recruited who was divided into two groups: a GJH group (Beighton score ≥ 4, n = 54) and a normal group (Beighton score < 4, n = 435). A paper questionnaire with questions about the participants' demographic characteristics and musculoskeletal disorder symptoms was collected. A three-dimensional gait analysis system was used to collect the participants' knee joint kinematic parameters during treadmill walking. Variables were evaluated using independent t-tests and Wilcoxon signed-rank tests.

Results: The prevalence of GJH was found to be 11.0 % among college students. Participants with GJH exhibited a greater active range of motions in the anterior/posterior translation than the normal (P =  0.026). Participants with GJH exhibited greater flexion at the end of the terminal stance (P = 0.039) and greater anterior translation of the tibia during almost the whole gait period than the normal group (P<0.05) during the treadmill gait. A greater external angle was found in GJH group during the periods of middle stance (P = 0.008).

Significance: GJH with a prevalence of 11.0 % among college students should be paid attention. Poor active motion stability in anterior/posterior translation may play an important role in the development of knee joint instability, potentially resulting in subsequent ACL deficiency and the development of knee osteoarthritis among people with GJH.
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http://dx.doi.org/10.1016/j.gaitpost.2020.12.002DOI Listing
February 2021

AChRs Degeneration at NMJ in Aging-Associated Sarcopenia-A Systematic Review.

Front Aging Neurosci 2020 10;12:597811. Epub 2020 Dec 10.

Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China.

Sarcopenia is an aging process with a decline of skeletal muscle mass and function, which is a challenging public health problem with reduced quality of life in patients. The endplate, the post-synaptic part of the neuromuscular junction (NMJ), occupies 0.1% of the myofiber surface area only, but is composed of millions of acetylcholine receptors (AChRs) that are efficient in binding to acetylcholine (ACh) and triggering skeletal muscle contraction. This systematic review aims to examine aging-associated alterations of post-synaptic AChRs, including morphology, function and related gene expression. A systematic literature search was conducted in PubMed, Embase and Web of Science with relevant keywords by two independent reviewers. Original pre-clinical and clinical studies regarding AChRs changes during aging with available full text and written in English were included. Information was extracted from the included studies for further review. In total, 30 articles were included. Various parameters assessing AChRs alterations by radioassay, immunofluorescence, electrophysiology and mechanical test were reported. Endplate fragmentation and denervation were common in old skeletal muscles during aging. To ensure efficient NMJ transmission and force generation, type I or IIb muscle fibers tended to have increased ACh quanta releasing after electrical stimulations, while type IIa muscle fibers tended to have stronger binding between ACh and AChRs, but the overall function of AChRs was reduced during aging. Alterations of AChRs area depended on muscle type, species and the progress of muscle atrophy and type I muscles fibers tended to demonstrate enlarging AChRs areas. Myogenic regulator factors (MRFs) can regulate the expression of AChRs subunits, while decreased MRF4 may lead to expression changes of AChRs subunits during aging. Sarcoglycan-α can delay low-density lipoprotein receptor-related protein 4 (LRP4) degradation. This protein was increased in old muscles but still cannot suppress the degradation of LRP4. Investigating the role of these AChRs-related genes in the process of aging may provide a potential target to treat sarcopenia.
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http://dx.doi.org/10.3389/fnagi.2020.597811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759742PMC
December 2020

A phase 1b expansion study of TAS-102 with oxaliplatin for refractory metastatic colorectal cancer.

Cancer 2021 May 22;127(9):1417-1424. Epub 2020 Dec 22.

Rutgers Cancer Institute of New Jersey, New Brunswick, New Jersey.

Background: TAS-102, a novel antimetabolite, is approved for treatment of refractory metastatic colorectal cancer (CRC). This study sought to determine whether the addition of TAS-102 to oxaliplatin (TAS-OX) was safe and effective in metastatic CRC previously treated with oxaliplatin.

Methods: This investigator-initiated, open-label, single-arm phase 1b study enrolled patients with metastatic CRC previously treated with 5-fluorouracil, irinotecan, and oxaliplatin. In dose escalation, TAS-102 was given at 3 dose levels: 25, 30, and 35 mg/m twice daily on day 1 to day 5 with 85 mg/m oxaliplatin on day 1 in 14-day cycles. The primary endpoint of dose escalation was the recommended dose for expansion, and in dose expansion, the primary endpoint was overall response rate (ORR) according to the Response Evaluation Criteria in Solid Tumors (RECIST, version 1.1).

Results: Forty-one patients were treated with TAS-OX. No dose-limiting toxicities were observed in the 11 patients treated in escalation. The recommended dose for expansion was 35 mg/m TAS-102 twice daily on day 1 to day 5 in combination with 85 mg/m oxaliplatin on day 1 in 14-day cycles. In the intention-to-treat population, the ORR was 2.4% (95% CI, 0%-12.9%) with 1 of 41 patients having a partial response, although 12 (29%) had tumor shrinkage. The median progression-free survival was 2.7 months (95% CI, 2.4-4.8 months) and median overall survival was 6.8 months (95% CI, 5.7-10 months).

Conclusions: TAS-OX is safe with no unexpected toxicities at standard doses of each agent. The combination did not result in a clinically meaningful ORR, although progression-free survival and overall survival were encouraging in this heavily pretreated population.

Lay Summary: For metastatic colorectal cancer, the treatment combination of TAS-102 and oxaliplatin was found to be well-tolerated and revealed no unexpected side effects. Twelve of 41 patients had reductions in the size of their tumor, and the study treatment delayed the time to tumor growth as opposed to what would be expected.
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http://dx.doi.org/10.1002/cncr.33379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085021PMC
May 2021

Associations between autoimmune diseases and amyotrophic lateral sclerosis: a register-based study.

Amyotroph Lateral Scler Frontotemporal Degener 2021 05 17;22(3-4):211-219. Epub 2020 Dec 17.

Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

To assess the associations of 43 autoimmune diseases with the subsequent risk of ALS and further evaluate the contribution of familial confounding to these associations. We conducted a nationwide register-based nested case-control study including 3561 ALS patients diagnosed during 1990-2013 in Sweden and 35,610 controls that were randomly selected from the general population and individually matched to the cases on age, sex, and county of birth. To evaluate the contribution of familial factors on the studied association, we additionally studied the first-degree relatives (siblings and children) of ALS patients and their controls. Patients with ALS had a 47% higher risk of being previously diagnosed with autoimmune disease (OR 1.47, 95% confidence interval [CI] 1.31-1.64), compared with controls. A positive association was noted for several autoimmune diseases, including myasthenia gravis, polymyositis or dermatomyositis, Guillain-Barre syndrome, type 1 diabetes diagnosed younger than 30 years, multiple sclerosis, and hypothyreosis. The increased risk of any autoimmune disease was greatest during the year before ALS diagnosis, likely due to misdiagnosis. A statistically significantly increased risk was also noted during 2-5 years, but not earlier, before ALS diagnosis. First-degree relatives of ALS patients had however no increased risk of autoimmune diseases compared with first-degree relatives of controls. Although it is difficult to completely remove the potential effects of misdiagnosis, there is likely a positive association between autoimmune disease (such as type 1 diabetes and multiple sclerosis) and ALS, which is not fully explained by shared familial confounding factors.
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http://dx.doi.org/10.1080/21678421.2020.1861022DOI Listing
May 2021

Erratum: Zhao, J.; Shen, X.; Cao, X.; He, H.; Han, S.; Chen, Y.; Cui, C.; Wei, Y.; Wang, Y.; Li, D.; Zhu, Q.; Yin, H. HDAC4 Regulates the Proliferation, Differentiation and Apoptosis of Chicken Skeletal Muscle Satellite Cells. 2020, , 84.

Animals (Basel) 2020 Dec 7;10(12). Epub 2020 Dec 7.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

The authors wish to make the following corrections to their paper [...].
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http://dx.doi.org/10.3390/ani10122322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762422PMC
December 2020

Tumor response prediction in Y radioembolization with PET-based radiomics features and absorbed dose metrics.

EJNMMI Phys 2020 Dec 9;7(1):74. Epub 2020 Dec 9.

Department of Radiology, University of Michigan, Ann Arbor, MI, USA.

Purpose: To evaluate whether lesion radiomics features and absorbed dose metrics extracted from post-therapy Y PET can be integrated to better predict outcomes in microsphere radioembolization of liver malignancies METHODS: Given the noisy nature of Y PET, first, a liver phantom study with repeated acquisitions and varying reconstruction parameters was used to identify a subset of robust radiomics features for the patient analysis. In 36 radioembolization procedures, Y PET/CT was performed within a couple of hours to extract 46 radiomics features and estimate absorbed dose in 105 primary and metastatic liver lesions. Robust radiomics modeling was based on bootstrapped multivariate logistic regression with shrinkage regularization (LASSO) and Cox regression with LASSO. Nested cross-validation and bootstrap resampling were used for optimal parameter/feature selection and for guarding against overfitting risks. Spearman rank correlation was used to analyze feature associations. Area under the receiver-operating characteristics curve (AUC) was used for lesion response (at first follow-up) analysis while Kaplan-Meier plots and c-index were used to assess progression model performance. Models with absorbed dose only, radiomics only, and combined models were developed to predict lesion outcome.

Results: The phantom study identified 15/46 reproducible and robust radiomics features that were subsequently used in the patient models. A lesion response model with zone percentage (ZP) and mean absorbed dose achieved an AUC of 0.729 (95% CI 0.702-0.758), and a progression model with zone size nonuniformity (ZSN) and absorbed dose achieved a c-index of 0.803 (95% CI 0.790-0.815) on nested cross-validation (CV). Although the combined models outperformed the radiomics only and absorbed dose only models, statistical significance was not achieved with the current limited data set to establish expected superiority.

Conclusion: We have developed new lesion-level response and progression models using textural radiomics features, derived from Y PET combined with mean absorbed dose for predicting outcome in radioembolization. These encouraging, but limited results, will need further validation in independent and larger datasets prior to any clinical adoption.
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http://dx.doi.org/10.1186/s40658-020-00340-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726084PMC
December 2020

Positive effects of resilience and self-efficacy on World Health Organization Quality of Life Instrument score among caregivers of stroke inpatients in China.

Psychogeriatrics 2021 Jan 9;21(1):89-99. Epub 2020 Dec 9.

Department of Social Medicine, School of Public Health, China Medical University, Shenyang, China.

Aim: Stroke is one of the major health challenges affecting life expectancy and quality of life around the world. However, there is limited reporting on the status of some caregivers, including Chinese caregivers of stroke inpatients. Limited information is available on evaluations using the World Health Organization Quality of Life Instrument (WHOQOL) and the effects of resilience and self-efficacy on WHOQOL score. Therefore, we conducted research to assess the role of resilience on the WHOQOL and to investigate the role of self-efficacy as a mediator between resilience and WHOQOL score among Chinese caregivers of stroke inpatients.

Methods: This cross-sectional study to gather data from north-east and south-east China was conducted from June 2019 to October 2019. Over 380 caregivers of stroke inpatients at two general public hospitals were interviewed face-to-face. About 305 caregivers (80.26%) completed the questionnaire, which included the Ego Resilience Scale, the General Self-Efficacy Scale, and the brief version of the WHOQOL and asked about demographic characteristics. This study also examined factors associated with WHOQOL score and used linear regression analysis and structure equation modelling to construct direct and indirect models, respectively.

Results: After adjustment for demographic characteristics, both resilience and self-efficacy were positively associated with all WHOQOL domains. Structure equation modelling revealed that self-efficacy mediated the relationship between resilience and WHOQOL score among caregivers of stroke inpatients.

Conclusion: Chinese caregivers of stroke inpatients exhibited good social health but poor physical, psychological, and environmental health. It is necessary for promoting resilience and improving WHOQOL score through the mediating effect of self-efficacy. These results suggest that interventions in health care focused on both enhancing resilience and providing self-efficacy training could effectively improve WHOQOL score.
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http://dx.doi.org/10.1111/psyg.12635DOI Listing
January 2021

Circular RNA CircFAM188B Encodes a Protein That Regulates Proliferation and Differentiation of Chicken Skeletal Muscle Satellite Cells.

Front Cell Dev Biol 2020 6;8:522588. Epub 2020 Nov 6.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Circular RNAs (circRNAs) are recognized as functional non-coding transcripts; however, emerging evidence has revealed that some synthetic circRNAs generate functional peptides or proteins. Additionally, the diverse biological functions of circRNAs include acting as miRNA-binding sponges, RNA-binding protein regulators, and protein translation templates. Previously, we found that circular RNA circFAM188B is a stable circular RNA and differentially expressed between broiler chickens and layers during embryonic skeletal muscle development. In this study, we found that circFAM188B exhibited a unique pattern of sharply decreased expression from embryonic day 10 (E10) to Day 35 (D35) after hatching. Our experimental results showed that circFAM188B promotes the proliferation, but inhibits the differentiation of chicken skeletal muscle satellite cells (SMSCs). Bioinformatic analysis revealed circFAM188B contain an opening reading frame (ORF) which translate into circFAM188B-103aa, internal ribosome entry site (IRES) analysis further confirmed the coding potential of circFAM188B. In addition, western blot assay detected a flag tagged circFAM188B-103aa, and several peptides of circFAM188B-103aa were detected by LC-MS/MS analysis. We further verified that the role of circFAM188B-103aa in chicken myogenesis is consistent with that of its parent transcript circFAM188B, which facilitates proliferation, but represses differentiation of chicken SMSC. Taken together, these results suggested that a novel protein circFAM188B-103aa encoded by circFAM188B that promotes the proliferation but inhibits the differentiation of chicken SMSCs.
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http://dx.doi.org/10.3389/fcell.2020.522588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677141PMC
November 2020

RiPerC Attenuates Cerebral Ischemia Injury through Regulation of miR-98/PIK3IP1/PI3K/AKT Signaling Pathway.

Oxid Med Cell Longev 2020 5;2020:6454281. Epub 2020 Oct 5.

Department of Anesthesiology, The University of Hong Kong, Hong Kong, SAR, China.

Background: Cerebral ischemic stroke is a refractory disease which seriously endangers human health. Remote ischemic perconditioning (RiPerC) by which the sublethal ischemic stimulus is administered during the ischemic event is beneficial after an acute stroke. However, the regulatory mechanism of RiPerC that relieves cerebral ischemic injury is still not completely clear.

Methods: In the present study, we investigated the regulatory mechanism of RiPerC in a rat model of ischemia induced by the middle cerebral artery occlusion (MCAO). Forty-eight adult male Sprague-Dawley (SD) rats were injected intracerebroventricularly with miR-98 agomir, miR-98 antagomir, or their negative controls (agomir-NC, antagomir-NC) 2 h before MCAO or MCAO+RiPerC followed by animal behavior tests and infraction volume measurement at 24 h after MCAO. The expression of miR-98, PIK3IP1, and tight junction proteins in rat hippocampus and cerebral cortex tissues was detected by quantitative polymerase chain reaction (qPCR) and Western blot (WB). Enzyme-linked immunosorbent assay (ELISA) was used to assess the IL-1, IL-6, and TNF- levels in the rat serum.

Results: The results showed that in MCAO group, the expression of PIK3IP1 was upregulated, but decreased after RiPerC treatment. Then, we found that PIK3IP1 was a potential target of miR-98. Treatment with miR-98 agomir decreased the infraction volume, reduced brain edema, and improved neurological functions compared to control rats. But treating with miR-98 antagomir in RiPerC group, the protective effect on cerebral ischemia injury was canceled.

Conclusion: Our finding indicated that RiPerC inhibited the MCAO-induced expression of PIK3IP1 through upregulated miR-98, thereby reducing the apoptosis induced by PIK3IP1 through the PI3K/AKT signaling pathway, thus reducing the cerebral ischemia-reperfusion injury.
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http://dx.doi.org/10.1155/2020/6454281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559836PMC
May 2021

Efficacy of modified rubber band ligation in the treatment of grade III internal hemorrhoids.

Ann Palliat Med 2021 Feb 12;10(2):1191-1197. Epub 2020 Oct 12.

Department of Coloproctology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Traditional rubber band ligation can improve the symptoms of hemorrhoids, the techniques used vary among centers and the degree of hemorrhoids may also affect the therapeutic efficacy and postoperative outcome, especially for patients with grade III hemorrhoids (hemorrhoid prolapses). This study aimed to investigate the clinical efficacy of modified rubber band ligation (MRBL) in the treatment of grade III internal hemorrhoids.

Methods: A total of 120 patients with grade III internal hemorrhoids were randomly assigned to receive MRBL or Milligan-Morgan haemorrhoidectomy (MMH) (n=60 per group). The post-operative pain, bleeding, urine retention and feeling of anal distension were recorded, and the resting anal pressure (RAP) and post-operative recurrence rate were compared between two groups.

Results: The post-operative pain, bleeding and urine retention in the MRBL group were improved significantly as compared with the MMH group (P<0.05), but the feeling of anal distension was similar between them (P>0.05). The RAP remained unchanged after MRBL, but the RAP at 1 month after surgery in the MMH group increased markedly (P<0.01) as compared with that before surgery and was significantly higher than that in the MRBL group (P<0.01). The post-operative recurrence rate was comparable between two groups (P>0.05).

Conclusions: As compared with traditional MMH, MRBL is effective to attenuate the post-operative pain and other discomforts and stabilize the RA. Thus, MRBL is an ideal choice for the treatment of grade III internal hemorrhoids.
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http://dx.doi.org/10.21037/apm-19-657DOI Listing
February 2021