Publications by authors named "Camila Fatima Rutkoski"

3 Publications

  • Page 1 of 1

Morphological, behavioral and genotoxic effects of glyphosate and 2,4-D mixture in tadpoles of two native species of South American amphibians.

Environ Toxicol Pharmacol 2021 Jul 19;85:103637. Epub 2021 Mar 19.

Laboratory of Ecology and Conservation, Federal University of Fronteira Sul, Erechim Campus, RS-135 - Km 72, nº 200, Erechim, RS, Brazil. Electronic address:

Pesticide contamination is an important factor in the global decline of amphibians. The herbicides glyphosate and 2,4-D are the most applied worldwide. These herbicides are often found in surface waters close to agricultural areas. This study aims at evaluating the chronic effects caused by glyphosate + 2,4-D mixture in Boana faber and Leptodactylus latrans tadpoles. The combined solution of the glyphosate and 2,4-D, in 5 different concentrations, was applied for 168 h. Herbicide mixtures did not affect the survival of the exposed tadpoles but growth and swimming activity were altered; besides causing several damages in the mouth and intestine. The erythrocytes showed micronuclei and other nuclear abnormalities. There is an ecological risk in the exposure of tadpoles of B. faber and L. latrans from the mixture of glyphosate + 2,4-D. Therefore, the approach used in this study provides important information on how commonly used pesticides can affect non-target organisms.
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http://dx.doi.org/10.1016/j.etap.2021.103637DOI Listing
July 2021

Genotoxic effects of glyphosate on Physalaemus tadpoles.

Environ Toxicol Pharmacol 2021 Jan 17;81:103516. Epub 2020 Oct 17.

Laboratory of Ecology and Conservation, Federal University of Fronteira Sul, Erechim Campus, Brazil, ERS 135 - Km 72, nº 200, Erechim, RS, Brazil. Electronic address:

Genotoxicity studies have revealed that pesticides bind to genetic material in non-target vertebrates, thereby impairing the genetic integrity of these animals. The main objective of this study was to determine the genotoxic damage in erythrocytes of two native South American amphibian Physalaemus cuvieri and Physalaemus gracilis, both species exposed to a glyphosate-based herbicide. We evaluated the presence of micronuclei (MN) and erythrocyte nuclear abnormalities (ENA) as biomarkers for potential genotoxic compounds. Tadpoles were exposed to doses permitted by Brazilian legislation and concentrations found naturally in Brazilian and Argentinian waters (500, 700 and 1000 μg/L). Glyphosate-based herbicide caused micronuclei formation and several types of erythrocyte nuclear abnormalities in both Physalaemus species. The total frequency of MN and ENA demonstrated the occurrence of cell damage at all tested concentrations. Glyphosate herbicide can be considered a genotoxic that may impact the genetic integrity of native populations of P. cuvieri and P. gracilis.
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http://dx.doi.org/10.1016/j.etap.2020.103516DOI Listing
January 2021

Can environmental concentrations of glyphosate affect survival and cause malformation in amphibians? Effects from a glyphosate-based herbicide on Physalaemus cuvieri and P. gracilis (Anura: Leptodactylidae).

Environ Sci Pollut Res Int 2020 Jun 21;27(18):22619-22630. Epub 2020 Apr 21.

Laboratory of Ecology and Conservation, Federal University of Fronteira Sul, Erechim Campus, ERS 135 - Km 72, n°200, Erechim, RS, Brazil.

Herbicides are the most common agrochemicals used in crops. Among them, glyphosate is the most widely applied in the world. Herbicides, especially organophosphates, have been shown to be hazardous to non-target species, including amphibians. The present study evaluated the acute and chronic effects of glyphosate-based herbicide (GBH), Roundup original® DI on tadpoles from two South American native species, Physalaemus cuvieri and P. gracilis. Spawnings were collected in the natural environment and maintained in the laboratory under controlled conditions. Acute and chronic toxicology trials began at stage 25 of Gosner (Herpetological 16:183-190, 1960). In an acute toxicity assay, seven GBH concentrations between 100 and 4500 μg a.e./L were tested over 96 h. For the chronic trials, tadpoles were subjected to both doses allowed by Brazilian legislation and to concentrations found in natural environment waters from Brazil and Argentina, between 65 and 1000 μg a.e/L over 14 days. Glyphosate had lethal effects on both studied species. Tadpoles showed shorter lengths and lower masses; that is, those that survived suffered chronic effects on growth and weight. The GBH maximum acceptable toxicant concentration for mortality and malformation was lower than the allowed level for Brazilian waters. The GBH tested in this study presented a high environmental and acute risk for the two studied species.
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http://dx.doi.org/10.1007/s11356-020-08869-zDOI Listing
June 2020
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