Publications by authors named "C Zhang"

48,580 Publications

Biodegraded PCl and gelatin fabricated vascular patch in rat aortic and inferior vena cava angioplasty.

Microvasc Res 2022 Jan 12:104314. Epub 2022 Jan 12.

Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China.

Novel synthetic prosthesis materials for patch angioplasty are continuously under development and optimization. When a nonwoven-based gelatin membrane is coupled with an electrospun layer of polycaprolactone (PCL), these biohybrid polymer membranes (BHMs) possess higher mechanical properties in aqueous environments. We hypothesized that BHMs can also be used as vascular patches, and we tested our hypothesis in a rat IVC venoplasty and aortic arterioplasty model. Patch venoplasty and arterioplasty were performed in SD rats (200 g), the patches were harvested at day 14, and samples were analyzed by immunohistochemistry and immunofluorescence. The BHM patches were almost degraded, with few parts remaining after 14 days. There was a line of CD34- and nestin-positive cells on the endothelium, with some cells CD34 and nestin dual-positive, macrophages and leukocytes also participated in the patch healing process. There were PCNA-positive cells in the neointima and peri-patch area, with some cells also PCNA and α-actin dual-positive. Arterial neointimal endothelial cells were Ephrin-B2- and dll-4-positive, and venous neointimal endothelial cells were Eph-B4- and COUP-TFII-positive. BHM shares a similar healing process like other patch materials, and BHM may have potential applications in vascular surgery.
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http://dx.doi.org/10.1016/j.mvr.2022.104314DOI Listing
January 2022

Biogeochemical transformation of sulfur and its effects on arsenic mobility in paddy fields polluted by acid mine drainage.

Chemosphere 2022 Jan 12:133605. Epub 2022 Jan 12.

College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang, 550025, China.

This study aimed to study the biogeochemical behaviour of sulfur and its potential impact on arsenic in farmland polluted by acid mine drainage (AMD). Soil samples were collected from paddy fields that were near arsenic-rich coal mines. Different geochemical analysis technologies, combined with the study of microbial populations, were used to analyse the enrichment and transformation characteristics of exogenous sulfur in the soil profile as well as their coupling effect with arsenic transformation. The results showed that sulfur, iron, and arsenic were obviously enriched in the soil, and sulfur easily migrated to deeper layers. The content of arsenic in contaminated soil was about 1.30-3.14 times higher compared to standard Chinese soil quality. Polluted soil had a higher content of bioavailable sulfur and adsorbed sulfur accounted for about 29% of the total sulfur. This promoted an increased microbial population abundance of Desulfosporosinus. The δS values of the polluted soil were lower than the background, ranging from -0.69‰ to 11.44‰, but were higher than the theoretical values. There was evidence of dissimilatory sulfur reduction and enrichment in S. A significant positive correlation was observed between the contents of adsorbed sulfur and total arsenic. The biogeochemical transformation of sulfur was conducive to the transformation of iron oxide-bound arsenic into sulfide minerals, which enhanced its stability. These results provide theoretical support for understanding the cycling transformation and environmental impact of sulfur in paddy fields polluted by AMD.
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http://dx.doi.org/10.1016/j.chemosphere.2022.133605DOI Listing
January 2022

Tc-labeled peptide targeting interleukin 13 receptor α 2 for tumor imaging in a cervical cancer mouse model.

Ann Nucl Med 2022 Jan 15. Epub 2022 Jan 15.

Department of Nuclear Medicine, Peking University First Hospital, No.8, Xishiku Street, Xicheng District, Beijing, 100034, China.

Objective: Pep-1 (CGEMGWVRC) can potently bind to interleukin 13 receptor α 2 (IL-13Rα2), a tumor-restricted receptor found to be expressed in various malignancies. In this study, we intended to prepare a Tc-labeled probe and evaluate its in vivo tumor accumulation properties in a cervical cancer xenograft model.

Methods: The Pep-1 was designed and radiolabeled with Tc by conjugation with mercaptoacetyl-triglycine (MAG). The labeling yield, radiochemical purity and stability were characterized in vitro. Cell uptake assays and fluorescence imaging were conducted for qualitative and quantitative evaluation of the specificity and affinity of Pep-1. Flow cytometry and tissue immunofluorescence were used to confirm the IL-13Rα2 expression in cervical cancer. Biodistribution and in vivo imaging were performed periodically to evaluate the imaging value of Tc-MAG-Pep-1 in cervical cancer xenograft model.

Results: Tc-MAG-Pep-1 was successfully prepared with a high labeling yield and radiochemical purity (> 95%). Specific cell uptake was demonstrated by scramble control and unlabeled MAG-Pep-1 blockade. Flow cytometry and tissue immunofluorescence also confirmed the mild IL-13Rα2 expression of HeLa. In the gamma imaging study and biodistribution, the tumors were imaged clearly at 2-6 h after injection of Tc-MAG-Pep-1 and the accumulation of Tc-MAG-Pep-1 in tumor was significantly higher than that in the blocking and scramble controls, demonstrating ligand-receptor binding specificity.

Conclusions: This work demonstrated that Tc-MAG-Pep-1 can bind to cervical cancer with high affinity and specificity. MAG-Pep-1 may be a prospective precursor for IL-13Rα2-expressing cancer therapy.
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http://dx.doi.org/10.1007/s12149-022-01715-xDOI Listing
January 2022

Advenella mandrilli sp. nov., a bacterium isolated from the faeces of Mandrillus sphinx.

Antonie Van Leeuwenhoek 2022 Jan 15. Epub 2022 Jan 15.

School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.

A novel Gram-negative strain WQ 585, isolated from the faeces of mandrills (Mandrillus sphinx) collected at Yunnan Wild Animal Park, Yunnan province, China, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Advenella, sharing 98.5% and 98.2% sequence similarity with the type strain Advenella alkanexedens LAM0050 and Advenella faeciporci M-07, respectively. The predominant ubiquinone was Q-8. The major cellular fatty acids (> 10%) were C C cyclo and Summed Feature 2. The G + C content of the genomic DNA of strain WQ 585 was 49.0%. The whole genome average nucleotide identity (gANI) values of strain WQ 585 with strain A. alkanexedens LAM0050 and A. faeciporci M-07 were 86.7% and 86.7%, and the digital DNA-DNA hybridization values of strain WQ 585 with strain A. alkanexedens LAM0050 and A. faeciporci M-07 were 64.5% and 62.5%, respectively. Growth occurred at 10-45 °C (optimally at 20-30 °C), pH 6.0-9.0 (optimally at pH 7.0), and 0-5% (w/v) NaCl (optimally at 0.5-2.0%). On the basis of the taxonomic evidence, a novel species, Advenella mandrilli sp. nov., is proposed. The type strain is WQ 585 (= KCTC 82396 = CCTCC AA 2020028).
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http://dx.doi.org/10.1007/s10482-021-01695-4DOI Listing
January 2022

miRNAs derived from plasma small extracellular vesicles predict organo-tropic metastasis of gastric cancer.

Gastric Cancer 2022 Jan 15. Epub 2022 Jan 15.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, 52 Fucheng Road, Hai-Dian District, Beijing, 100142, China.

Background: Peritoneum, liver and lymph node are the most common metastatic sites of gastric cancer (GC). Biomarkers for GC's organo-tropic metastasis remained largely unknown, which was investigated in this study from the perspective of small extracellular vesicle (sEV)-derived miRNAs.

Methods: Plasma from treatment-naïve GC patients including no metastasis (M0), peritoneal metastasis (PM), hepatic metastasis (HM) and distant lymph node metastasis (dLNM)) were divided into one discovery (N = 40), one training (N = 40) and one validating cohort (N = 86), then assessed by sEV-miRNA-sequencing and sEV-miRNA-qPCR. Functional explorations were also performed for verification.

Results: The expression profiles of sEV-miRNAs varied greatly across different metastatic patterns. Based on logistic regression models, we constructed signatures for M0 (hsa-miR-186-5p/hsa-miR-200c-3p/hsa-miR-429/hsa-miR-5187-5p/hsa-miR-548ae-5p), PM (hsa-miR-200c-3p/hsa-miR-429), HM (hsa-miR-200c-3p/hsa-miR-429) and dLNM (hsa-miR-324-5p/hsa-miR-374a-5p/hsa-miR-429/hsa-miR-548ae-5p). These signatures vigorously characterized organo-tropic metastasis (all displaying AUC > 0.8, consistency ≥ 75%), and effectively conjectured the risk of future metastasis within 5 years (accuracy 45.5% for occurrence, 70% for organotropism, P = 0.002 for prognostic diversity). Additionally, we explored these seven biomarker miRNAs' impact on GC's in vitro motility and discussed their potential involvement in cancer-related biological processes and pathways.

Conclusions: Our work highlighted that plasma sEV-miRNAs powerfully characterized and predicted the organo-tropic metastasis of GC and provided new insight into the applications of sEV-based liquid biopsy in clinical practice.
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http://dx.doi.org/10.1007/s10120-021-01267-5DOI Listing
January 2022
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