Publications by authors named "C Wang"

71,310 Publications

Micromagnetic simulation of microstructure effect for binary-main-phase Nd-Ce-Fe-B magnets.

J Phys Condens Matter 2021 Aug 4. Epub 2021 Aug 4.

Peking University, Peking University, Hidian District, Beijing, Beiing, 100871, CHINA.

We investigate the magnetic properties of a chemically heterogeneous binary-main-phase (BMP) Nd-Ce-Fe-B magnet with a core-shell structure via micromagnetic simulation. It is found that the coercivity strongly depends on the shell thickness. The BMP magnet's coercivityinitially increases and then decreases with increasing Nd-rich shell thickness, and so there is the optimal shell thickness which shows the maximum coercivity for any given Ce concentration. The simulation shows the significant difference in coercivity and maximum energy product between the BMP and single-main-phase(SMP) magnets. Notably, the magnetization reversal mechanism of the BMP magnet is revealed in the simulation. Local reversals in the BMP magnet first occur in the Ce-rich shells, followed by the Nd-rich cores. Then, the magnetization in Ce-rich core/Nd-rich shell typed grains is switched after reversed magnetization of all the Nd-rich core/Ce-rich shell typed grains. The BMP magnet represents a further increased coercivity for a larger GB thickness, which can be well explained by a maximum stray field.
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http://dx.doi.org/10.1088/1361-648X/ac1aa1DOI Listing
August 2021

Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancer.

Cell Rep 2021 Aug;36(5):109491

Department of Medicine, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA; UCSF Helen Diller Comprehensive Cancer Center, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA. Electronic address:

The exocyst is an evolutionarily conserved protein complex that regulates vesicular trafficking and scaffolds signal transduction. Key upstream components of the exocyst include monomeric RAL GTPases, which help mount cell-autonomous responses to trophic and immunogenic signals. Here, we present a quantitative proteomics-based characterization of dynamic and signal-dependent exocyst protein interactomes. Under viral infection, an Exo84 exocyst subcomplex assembles the immune kinase Protein Kinase R (PKR) together with the Hippo kinase Macrophage Stimulating 1 (MST1). PKR phosphorylates MST1 to activate Hippo signaling and inactivate Yes Associated Protein 1 (YAP1). By contrast, a Sec5 exocyst subcomplex recruits another immune kinase, TANK binding kinase 1 (TBK1), which interacted with and activated mammalian target of rapamycin (mTOR). RALB was necessary and sufficient for induction of Hippo and mTOR signaling through parallel exocyst subcomplex engagement, supporting the cellular response to virus infection and oncogenic signaling. This study highlights RALB-exocyst signaling subcomplexes as mechanisms for the integrated engagement of Hippo and mTOR signaling in cells challenged by viral pathogens or oncogenic signaling.
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http://dx.doi.org/10.1016/j.celrep.2021.109491DOI Listing
August 2021

Evaluation of gallnut tannin and Lactobacillus plantarum as natural modifiers for alfalfa silage: ensiling characteristics, in vitro ruminal methane production, fermentation profile and microbiota.

J Appl Microbiol 2021 Aug 4. Epub 2021 Aug 4.

College of Animal Science, Shanxi Agricultural University, 030801, Shanxi Province, China.

Aims: The potential of gallnut tannin (GT) and Lactobacillus plantarum (LP) on fermentation characteristics, in vitro ruminal methane production and microbiota of alfalfa silage was investigated.

Methods And Results: Alfalfa was ensiled with GT [20 and 50 g kg dry matter (DM)] and LP (3 × 10 cfu g fresh matter) alone or in combinations for 60 days. The GT and LP alone or in combinations decreased DM losses, pH, and non-protein nitrogen contents of alfalfa silage. All additive treatments decreased ruminal methane production, and increased propionic acid molar proportions and Fibrobacter succinogenes numbers. The LP treatment increased nutrient degradation, cellobiase, pectinase, and protease activities, and Prevotella ruminicola abundance, whereas high-dose GT treatment inhibited these variables. Importantly, LP together with GT alleviated the adverse effects of high-dose GT supply alone by enhancing pectinase and protease activities as well as Rumincoccus flavefaciens and P. ruminicola growth.

Conclusions: Combinations of GT and LP can be used as an efficient additive to improve silage quality and utilization by ruminants.

Significance: and Impact of study: Using GT-LP combinations has practical implications, particularly concerning effects of tannins on ruminal methane mitigation, which may alleviate inhibitory effects of tannins on feed digestion through modulating ruminal microbiota.
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http://dx.doi.org/10.1111/jam.15246DOI Listing
August 2021

Lapatinib and poziotinib overcome ABCB1-mediated paclitaxel resistance in ovarian cancer.

PLoS One 2021 4;16(8):e0254205. Epub 2021 Aug 4.

Markey Cancer Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America.

Conventional frontline treatment for ovarian cancer consists of successive chemotherapy cycles of paclitaxel and platinum. Despite the initial favorable responses for most patients, chemotherapy resistance frequently leads to recurrent or refractory disease. New treatment strategies that circumvent or prevent mechanisms of resistance are needed to improve ovarian cancer therapy. We established in vitro paclitaxel-resistant ovarian cancer cell line and organoid models. Gene expression differences in resistant and sensitive lines were analyzed by RNA sequencing. We manipulated candidate genes associated with paclitaxel resistance using siRNA or small molecule inhibitors, and then screened the cells for paclitaxel sensitivity using cell viability assays. We used the Bliss independence model to evaluate the anti-proliferative synergy for drug combinations. ABCB1 expression was upregulated in paclitaxel-resistant TOV-21G (q < 1x10-300), OVCAR3 (q = 7.4x10-156) and novel ovarian tumor organoid (p = 2.4x10-4) models. Previous reports have shown some tyrosine kinase inhibitors can inhibit ABCB1 function. We tested a panel of tyrosine kinase inhibitors for the ability to sensitize resistant ABCB1-overexpressing ovarian cancer cell lines to paclitaxel. We observed synergy when we combined poziotinib or lapatinib with paclitaxel in resistant TOV-21G and OVCAR3 cells. Silencing ABCB1 expression in paclitaxel-resistant TOV-21G and OVCAR3 cells reduced paclitaxel IC50 by 20.7 and 6.2-fold, respectively. Furthermore, we demonstrated direct inhibition of paclitaxel-induced ABCB1 transporter activity by both lapatinib and poziotinib. In conclusion, lapatinib and poziotinib combined with paclitaxel synergizes to inhibit the proliferation of ABCB1-overexpressing ovarian cancer cells in vitro. The addition of FDA-approved lapatinib to second-line paclitaxel therapy is a promising strategy for patients with recurrent ovarian cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254205PLOS
August 2021

Nickel-Catalyzed Asymmetric Reductive Dicarbamoylation of Alkenes.

Org Lett 2021 Aug 4. Epub 2021 Aug 4.

Hefei National Laboratory for Physical Science at the Microscale and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

Herein we report a nickel-catalyzed asymmetric reductive dicarbamoylation of alkenes, in which tethered carbamoyl chlorides and isocyanates serve as distinct electrophilic carbamoylating agents, providing new access to chiral oxindoles bearing an amide-substituted quaternary stereogenic center.
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http://dx.doi.org/10.1021/acs.orglett.1c02223DOI Listing
August 2021
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