Publications by authors named "C Baillie"

119 Publications

Chronic Iron Deficiency Anaemia Caused by Blue Rubber Bleb Syndrome (BRBS).

J Pediatr Gastroenterol Nutr 2021 Jul 20. Epub 2021 Jul 20.

University of Liverpool Alder Hey Children's NHS Foundation Trust, Liverpool, L12 2AP Glan Clwyd Hospital, Rhyl, Denbighshire, Wales, LL18 5UJ.

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http://dx.doi.org/10.1097/MPG.0000000000003242DOI Listing
July 2021

Sensory wellbeing workshops for inpatient and day-care patients with anorexia nervosa.

Neuropsychiatr 2021 Jun 15. Epub 2021 Jun 15.

Eating Disorders National Service, South London and Maudsley NHS Foundation Trust, London, UK.

Background: The wellbeing of patients with eating disorders is one of the priorities in the "bigger picture" of treatment for eating disorders. Sensory soothing strategies for sensory sensitivities are supportive tools which could be useful in day-care and inpatient clinical programmes.

Methods: Evaluation of multiple separate sensory wellbeing workshops consisting of psychoeducation and experiential components delivered in inpatient and intensive day-care services was performed. Participants' self-report questionnaires were evaluated pre- and post-workshop. Additionally, patients' comments and qualitative feedback was collected after completion of the workshop.

Results: There was strong evidence that self-reported awareness of sensory wellbeing, awareness of strategies to enhance sensory wellbeing, and confidence in managing sensory wellbeing increased after the workshops with positive qualitative feedback from participants. The feedback questionnaires highlighted that patients found the sessions useful and were able to use some of the skills and strategies they learned in the workshop.

Conclusion: This pilot work on sensory wellbeing workshops with a protocol-based format was feasible and beneficial for the patient group. Preliminary evidence suggests that delivery of similar workshops could be sensible in addition to treatment as usual in inpatient and day-care programmes.
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http://dx.doi.org/10.1007/s40211-021-00392-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204121PMC
June 2021

Generation of a chemical genetic model for JAK3.

Sci Rep 2021 May 12;11(1):10093. Epub 2021 May 12.

Division of Cell Signalling and Immunology, School of Life Sciences, Wellcome Trust Building, University of Dundee, Dundee, DD1 5EH, UK.

Janus Kinases (JAKs) have emerged as an important drug target for the treatment of a number of immune disorders due to the central role that they play in cytokine signalling. 4 isoforms of JAKs exist in mammalian cells and the ideal isoform profile of a JAK inhibitor has been the subject of much debate. JAK3 has been proposed as an ideal target due to its expression being largely restricted to the immune system and its requirement for signalling by cytokine receptors using the common γ-chain. Unlike other JAKs, JAK3 possesses a cysteine in its ATP binding pocket and this has allowed the design of isoform selective covalent JAK3 inhibitors targeting this residue. We report here that mutating this cysteine to serine does not prevent JAK3 catalytic activity but does greatly increase the IC50 for covalent JAK3 inhibitors. Mice with a Cys905Ser knockin mutation in the endogenous JAK3 gene are viable and show no apparent welfare issues. Cells from these mice show normal STAT phosphorylation in response to JAK3 dependent cytokines but are resistant to the effects of covalent JAK3 inhibitors. These mice therefore provide a chemical-genetic model to study JAK3 function.
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http://dx.doi.org/10.1038/s41598-021-89356-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115619PMC
May 2021

A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research.

PLoS Biol 2021 02 25;19(2):e3001091. Epub 2021 Feb 25.

MRC-University of Glasgow Centre for Virus Research (CVR), Glasgow, United Kingdom.

The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation. In response, many laboratories have redirected attention to SARS-CoV-2, meaning there is an urgent need for tools that can be used in laboratories unaccustomed to working with coronaviruses. Here we report a range of tools for SARS-CoV-2 research. First, we describe a facile single plasmid SARS-CoV-2 reverse genetics system that is simple to genetically manipulate and can be used to rescue infectious virus through transient transfection (without in vitro transcription or additional expression plasmids). The rescue system is accompanied by our panel of SARS-CoV-2 antibodies (against nearly every viral protein), SARS-CoV-2 clinical isolates, and SARS-CoV-2 permissive cell lines, which are all openly available to the scientific community. Using these tools, we demonstrate here that the controversial ORF10 protein is expressed in infected cells. Furthermore, we show that the promising repurposed antiviral activity of apilimod is dependent on TMPRSS2 expression. Altogether, our SARS-CoV-2 toolkit, which can be directly accessed via our website at https://mrcppu-covid.bio/, constitutes a resource with considerable potential to advance COVID-19 vaccine design, drug testing, and discovery science.
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http://dx.doi.org/10.1371/journal.pbio.3001091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906417PMC
February 2021

All is fish that comes to the net: metabarcoding for rapid fisheries catch assessment.

Ecol Appl 2021 03 20;31(2):e02273. Epub 2021 Jan 20.

School of Biological & Environmental Sciences, Liverpool John Moores University, Liverpool, United Kingdom.

Monitoring marine resource exploitation is a key activity in fisheries science and biodiversity conservation. Since research surveys are time consuming and costly, fishery-dependent data (i.e., derived directly from fishing vessels) are increasingly credited with a key role in expanding the reach of ocean monitoring. Fishing vessels may be seen as widely ranging data-collecting platforms, which could act as a fleet of sentinels for monitoring marine life, in particular exploited stocks. Here, we investigate the possibility of assessing catch composition of single hauls carried out by trawlers by applying DNA metabarcoding to the dense water draining from fishing nets just after the end of hauling operations (hereafter "slush"). We assess the performance of this approach in portraying β-diversity and examining the quantitative relationship between species abundances in the catch and DNA amount in the slush (read counts generated by amplicon sequencing). We demonstrate that the assemblages identified using DNA in the slush satisfactorily mirror those returned by visual inspection of net content (about 71% of species and 86% of families of fish) and detect a strong relationship between read counts and species abundances in the catch. We therefore argue that this approach could be upscaled to serve as a powerful source of information on the structure of demersal assemblages and the impact of fisheries.
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http://dx.doi.org/10.1002/eap.2273DOI Listing
March 2021
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