Publications by authors named "Brian Raught"

100Publications

Proximal Protein Interaction Landscape of RAS Paralogs.

Cancers (Basel) 2020 Nov 11;12(11). Epub 2020 Nov 11.

CHU Montpellier, Department of Pathology and Onco-Biology, Univ Montpellier, 34295 Montpellier, France.

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November 2020

Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.

Dev Cell 2020 Oct 9;55(2):209-223.e7. Epub 2020 Oct 9.

Biochemistry Department, University of Toronto, Toronto, ON M5S1A8, Canada; Cell Biology Program, The Hospital for Sick Children, Toronto, ON M5G0A4, Canada; NIHR Southampton Biomedical Research Center, Clinical & Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK. Electronic address:

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October 2020

Mutations of the Transcriptional Corepressor ZMYM2 Cause Syndromic Urinary Tract Malformations.

Authors:
Dervla M Connaughton Rufeng Dai Danielle J Owen Jonathan Marquez Nina Mann Adda L Graham-Paquin Makiko Nakayama Etienne Coyaud Estelle M N Laurent Jonathan R St-Germain Lot Snijders Blok Arianna Vino Verena Klämbt Konstantin Deutsch Chen-Han Wilfred Wu Caroline M Kolvenbach Franziska Kause Isabel Ottlewski Ronen Schneider Thomas M Kitzler Amar J Majmundar Florian Buerger Ana C Onuchic-Whitford Mao Youying Amy Kolb Daanya Salmanullah Evan Chen Amelie T van der Ven Jia Rao Hadas Ityel Steve Seltzsam Johanna M Rieke Jing Chen Asaf Vivante Daw-Yang Hwang Stefan Kohl Gabriel C Dworschak Tobias Hermle Mariëlle Alders Tobias Bartolomaeus Stuart B Bauer Michelle A Baum Eva H Brilstra Thomas D Challman Jacob Zyskind Carrie E Costin Katrina M Dipple Floor A Duijkers Marcia Ferguson David R Fitzpatrick Roger Fick Ian A Glass Peter J Hulick Antonie D Kline Ilona Krey Selvin Kumar Weining Lu Elysa J Marco Ingrid M Wentzensen Heather C Mefford Konrad Platzer Inna S Povolotskaya Juliann M Savatt Natalia V Shcherbakova Prabha Senguttuvan Audrey E Squire Deborah R Stein Isabelle Thiffault Victoria Y Voinova Michael J G Somers Michael A Ferguson Avram Z Traum Ghaleb H Daouk Ankana Daga Nancy M Rodig Paulien A Terhal Ellen van Binsbergen Loai A Eid Velibor Tasic Hila Milo Rasouly Tze Y Lim Dina F Ahram Ali G Gharavi Heiko M Reutter Heidi L Rehm Daniel G MacArthur Monkol Lek Kristen M Laricchia Richard P Lifton Hong Xu Shrikant M Mane Simone Sanna-Cherchi Andrew D Sharrocks Brian Raught Simon E Fisher Maxime Bouchard Mustafa K Khokha Shirlee Shril Friedhelm Hildebrandt

Am J Hum Genet 2020 10 4;107(4):727-742. Epub 2020 Sep 4.

Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

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October 2020

Nucleotide Binding, Evolutionary Insights, and Interaction Partners of the Pseudokinase Unc-51-like Kinase 4.

Structure 2020 Nov 18;28(11):1184-1196.e6. Epub 2020 Aug 18.

Institute for Pharmaceutical Chemistry, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany; Buchmann Institute for Molecular Life Sciences, Structural Genomics Consortium, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 15, 60438 Frankfurt am Main, Germany; German Cancer Consortium (DKTK) and Frankfurt Cancer Institute (FCI), 60596 Frankfurt am Main, Germany. Electronic address:

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November 2020

Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.

J Proteome Res 2020 08 20;19(8):3554-3561. Epub 2020 Jul 20.

Princess Margaret Cancer Centre, University Health Network, University of Toronto, 101 College Street, Toronto, ON M5G 1L7, Canada.

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August 2020

The long form of pVHL is artifactually modified by serine protease inhibitor AEBSF.

Protein Sci 2020 08 25;29(8):1843-1850. Epub 2020 Jun 25.

Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada.

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August 2020

Multiple direct interactions of TBP with the MYC oncoprotein.

Nat Struct Mol Biol 2019 11 4;26(11):1035-1043. Epub 2019 Nov 4.

Department of Physics, Chemistry, and Biology, Linköping University, Linköping, Sweden.

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November 2019

BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion.

Mol Cell Proteomics 2019 Nov 15;18(11):2285-2297. Epub 2020 Sep 15.

Department of Cell Biology, University of Alberta, Edmonton, AB, T6G 2H7. Electronic address:

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November 2019

Proximity interactions of the ubiquitin ligase Mind bomb 1 reveal a role in regulation of epithelial polarity complex proteins.

Sci Rep 2019 08 28;9(1):12471. Epub 2019 Aug 28.

Program in Cell Biology and The Arthur and Sonia Brain Tumour Research Centre, The Hospital for Sick Children, 555 University Ave., Toronto, ON, M5G 1X8, Canada.

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August 2019

The Ion Transporter NKCC1 Links Cell Volume to Cell Mass Regulation by Suppressing mTORC1.

Cell Rep 2019 05;27(6):1886-1896.e6

Program in Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Biochemistry Department, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

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May 2019

FAM105A/OTULINL Is a Pseudodeubiquitinase of the OTU-Class that Localizes to the ER Membrane.

Structure 2019 06 2;27(6):1000-1012.e6. Epub 2019 May 2.

Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

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June 2019

Mitochondrial ClpP-Mediated Proteolysis Induces Selective Cancer Cell Lethality.

Cancer Cell 2019 05 2;35(5):721-737.e9. Epub 2019 May 2.

The University of Texas MD Anderson Cancer Center, Molecular Hematology and Therapy, Department of Leukemia, Houston, TX 77030, USA; The University of Texas MD Anderson Cancer Center; Department of Leukemia, Houston, TX 77030, USA. Electronic address:

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May 2019

LLGL2 rescues nutrient stress by promoting leucine uptake in ER breast cancer.

Nature 2019 05 17;569(7755):275-279. Epub 2019 Apr 17.

Department of Medicine, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

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May 2019

USP7 Regulates Cytokinesis through FBXO38 and KIF20B.

Sci Rep 2019 02 25;9(1):2724. Epub 2019 Feb 25.

Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

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February 2019

Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.

Curr Opin Chem Biol 2019 02 17;48:44-54. Epub 2018 Nov 17.

Princess Margaret Cancer Centre, University Health Network, and Department of Medical Biophysics, University of Toronto, 101 College Street, 9-701A, Toronto, ON, M5G 1L7, Canada.

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February 2019

MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis.

Mol Cell 2018 12 8;72(5):836-848.e7. Epub 2018 Nov 8.

Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1L7, Canada. Electronic address:

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December 2018

The SUMO-specific isopeptidase SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix.

Mol Biol Cell 2018 08 6;29(15):1878-1890. Epub 2018 Jun 6.

Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205.

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August 2018

Atypical Cadherin Dachsous1b Interacts with Ttc28 and Aurora B to Control Microtubule Dynamics in Embryonic Cleavages.

Dev Cell 2018 05;45(3):376-391.e5

Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address:

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May 2018

Spatiotemporal distribution of small ubiquitin-like modifiers during human placental development and in response to oxidative and inflammatory stress.

J Physiol 2018 05 6;596(9):1587-1600. Epub 2018 Apr 6.

Program in Development and Fetal Health, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, M5T3H7, Canada.

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May 2018

The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity.

Sci Rep 2017 03 7;7(1):107. Epub 2017 Mar 7.

Montreal Neurological Institute, McGill University, 3801 University Ave, Montreal, Quebec, H3A 2B4, Canada.

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March 2017

UbE2E1/UBCH6 Is a Critical E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119.

J Biol Chem 2017 02 10;292(7):2893-2902. Epub 2017 Jan 10.

From the Department of Biology, York University, Toronto, Ontario M3J1P3 and

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February 2017

Characterizing the mitochondrial DNA polymerase gamma interactome by BioID identifies Ruvbl2 localizes to the mitochondria.

Mitochondrion 2017 Jan 11;32:31-35. Epub 2016 Nov 11.

Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada; Department of Medical Biophysics, University of Toronto, ON, Canada. Electronic address:

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January 2017

Data Independent Acquisition analysis in ProHits 4.0.

J Proteomics 2016 10 29;149:64-68. Epub 2016 Apr 29.

Centre for Systems Biology, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. Electronic address:

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October 2016

RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation.

Nat Commun 2016 08 25;7:12638. Epub 2016 Aug 25.

Department of Medical Biophysics, Princess Margaret Cancer Centre, Ontario Cancer Institute, University Health Network, University of Toronto, Toronto, Ontario, Canada M5G 1L7.

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August 2016

ChromNet: Learning the human chromatin network from all ENCODE ChIP-seq data.

Genome Biol 2016 Apr 30;17:82. Epub 2016 Apr 30.

Department of Computer Science and Engineering, University of Washington, Seattle, WA, USA.

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April 2016

Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling.

J Biol Chem 2016 Apr 4;291(14):7357-72. Epub 2016 Feb 4.

From the Departments of Laboratory Medicine and Pathobiology and Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8 and

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April 2016

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.

Cell 2015 Dec;163(6):1484-99

Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

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December 2015

Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis.

Nat Commun 2015 Nov 30;6:8859. Epub 2015 Nov 30.

Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, M5S1A8 Ontario, Canada.

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November 2015

The Deubiquitinase USP37 Regulates Chromosome Cohesion and Mitotic Progression.

Curr Biol 2015 Aug 20;25(17):2290-9. Epub 2015 Aug 20.

Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address:

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August 2015

Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry.

Data Brief 2015 Mar 27;2:29-31. Epub 2014 Nov 27.

Princess Margaret Cancer Centre, University Health Network, Canada ; Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.

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March 2015

BioID data of c-MYC interacting protein partners in cultured cells and xenograft tumors.

Data Brief 2014 Dec 14;1:76-8. Epub 2014 Oct 14.

Princess Margaret Cancer Centre, University Health Network, and Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.

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December 2014

Fat1 interacts with Fat4 to regulate neural tube closure, neural progenitor proliferation and apical constriction during mouse brain development.

Development 2015 Aug 24;142(16):2781-91. Epub 2015 Jul 24.

Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

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August 2015

The use of ubiquitin lysine mutants to characterize E2-E3 linkage specificity: Mass spectrometry offers a cautionary "tail".

Proteomics 2015 Sep 2;15(17):2910-5. Epub 2015 Jul 2.

Princess Margaret Cancer Centre, University Health Network, and Department of Medical Biophysics, University of Toronto, Toronto, ON, CANADA.

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September 2015

SUMO1 Affects Synaptic Function, Spine Density and Memory.

Sci Rep 2015 May 29;5:10730. Epub 2015 May 29.

1] Tanz Centre for Research in Neurodegenerative Diseases, University of Toronto, 60 Leonard Avenue, Toronto, M5T 2S8, Ontario [2] Departments of Medical Biophysics, Laboratory Medicine and Pathobiology University of Toronto.

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May 2015

BioID-based Identification of Skp Cullin F-box (SCF)β-TrCP1/2 E3 Ligase Substrates.

Mol Cell Proteomics 2015 Jul 21;14(7):1781-95. Epub 2015 Apr 21.

From the ‡Princess Margaret Cancer Centre, University Health Network; ‡‡Department of Medical Biophysics, University of Toronto, Toronto, ON Canada

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July 2015

CHCHD2 Is Coamplified with EGFR in NSCLC and Regulates Mitochondrial Function and Cell Migration.

Mol Cancer Res 2015 Jul 17;13(7):1119-29. Epub 2015 Mar 17.

Program in Molecular Structure and Function, Hospital For Sick Children, Toronto, Ontario, Canada. Princess Margaret Cancer Centre, Toronto, Ontario, Canada. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

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July 2015

KCMF1 (potassium channel modulatory factor 1) Links RAD6 to UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4) and lysosome-mediated degradation.

Mol Cell Proteomics 2015 Mar 12;14(3):674-85. Epub 2015 Jan 12.

From the ‡Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto;

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March 2015

Identification of c-MYC SUMOylation by mass spectrometry.

PLoS One 2014 18;9(12):e115337. Epub 2014 Dec 18.

Princess Margaret Cancer Centre, University Health Network, Toronto, Canada; Department of Medical Biophysics, University of Toronto, Toronto, Canada.

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December 2015

BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors.

J Proteomics 2015 Apr 18;118:95-111. Epub 2014 Oct 18.

Princess Margaret Cancer Centre, University Health Network, and Department of Medical Biophysics, University of Toronto, Toronto, ON Canada. Electronic address:

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April 2015

The S. cerevisiae SUMO stress response is a conjugation-deconjugation cycle that targets the transcription machinery.

J Proteomics 2015 Apr 27;118:39-48. Epub 2014 Nov 27.

Princess Margaret Cancer Centre, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada. Electronic address:

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April 2015

Identification of SUMO-2/3-modified proteins associated with mitotic chromosomes.

Proteomics 2015 Feb 7;15(4):763-72. Epub 2015 Jan 7.

Department of Biochemistry & Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

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February 2015

Src promotes GTPase activity of Ras via tyrosine 32 phosphorylation.

Proc Natl Acad Sci U S A 2014 Sep 25;111(36):E3785-94. Epub 2014 Aug 25.

Departments of Laboratory Medicine and Pathobiology and Biochemistry, University of Toronto, Toronto, ON, Canada M5S1A8;

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September 2014

Myc and its interactors take shape.

Biochim Biophys Acta 2015 May 14;1849(5):469-83. Epub 2014 Jun 14.

Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada. Electronic address:

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May 2015

Proteomic analyses of CSF aimed at biomarker development for pediatric brain tumors.

J Neurooncol 2014 Jun 26;118(2):225-238. Epub 2014 Apr 26.

Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.

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June 2014

RNF168 ubiquitylates 53BP1 and controls its response to DNA double-strand breaks.

Proc Natl Acad Sci U S A 2013 Dec 9;110(52):20982-7. Epub 2013 Dec 9.

Ontario Cancer Institute, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, M5G 2M9 ON, Canada.

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December 2013

MYC phosphorylation at novel regulatory regions suppresses transforming activity.

Cancer Res 2013 Nov 12;73(21):6504-15. Epub 2013 Sep 12.

Authors' Affiliations: Department of Medical Biophysics, University of Toronto; Ontario Cancer Institute, Campbell Family Institute for Cancer Research, Princess Margaret Cancer Centre; and Ontario Institute for Cancer Research, Toronto, Ontario, Canada.

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November 2013

CEP120 and SPICE1 cooperate with CPAP in centriole elongation.

Curr Biol 2013 Jul 27;23(14):1360-6. Epub 2013 Jun 27.

Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON M5G 1X5, Canada.

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July 2013

A global S. cerevisiae small ubiquitin-related modifier (SUMO) system interactome.

Mol Syst Biol 2013 May 28;9:668. Epub 2013 May 28.

Ontario Cancer Institute, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.

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May 2013

A pathogen type III effector with a novel E3 ubiquitin ligase architecture.

PLoS Pathog 2013 Jan 24;9(1):e1003121. Epub 2013 Jan 24.

Banting and Best Department for Medical Research, University of Toronto, CH Best Institute, Toronto, Ontario, Canada.

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January 2013

Using ProHits to store, annotate, and analyze affinity purification-mass spectrometry (AP-MS) data.

Curr Protoc Bioinformatics 2012 Sep;Chapter 8:Unit8.16

Centre for Systems Biology, Samuel Lunenfeld Research Institute at Mount Sinai Hospital, Toronto, Ontario, Canada.

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September 2012

Beyond hairballs: The use of quantitative mass spectrometry data to understand protein-protein interactions.

FEBS Lett 2012 Aug 10;586(17):2723-31. Epub 2012 Apr 10.

Centre for Systems Biology, Samuel Lunenfeld Research Institute at Mount Sinai Hospital, Department of Molecular Genetics, University of Toronto, Canada.

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August 2012

The SUMO-specific isopeptidase SENP2 associates dynamically with nuclear pore complexes through interactions with karyopherins and the Nup107-160 nucleoporin subcomplex.

Mol Biol Cell 2011 Dec 26;22(24):4868-82. Epub 2011 Oct 26.

Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.

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December 2011

The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases.

J Biol Chem 2011 Mar 19;286(12):10238-47. Epub 2011 Jan 19.

Sanford-Burnham Medical Research Institute, La Jolla, California 92037, USA.

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March 2011

A novel mechanism for SUMO system control: regulated Ulp1 nucleolar sequestration.

Mol Cell Biol 2010 Sep 20;30(18):4452-62. Epub 2010 Jul 20.

Ontario Cancer Institute, University of Toronto, MaRS TMDT 9-805, 101 College Street, Toronto, Ontario M5G 1L7, Canada.

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September 2010

PML/RARA oxidation and arsenic binding initiate the antileukemia response of As2O3.

Cancer Cell 2010 Jul;18(1):88-98

Inserm/Centre National de la Recherche Scientifique (CNRS)/Université Paris Diderot/Institut Universitaire Hématologie U944/UMR7212, Laboratoire associé de la Ligue Nationale contre le Cancer, Hôpital St Louis, 1, Av. C. Vellefaux, 75475 Paris, Cedex 10, France.

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July 2010