Brad Snedecor

Brad Snedecor

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Brad Snedecor

Brad Snedecor

Publications by authors named "Brad Snedecor"

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19Publications

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Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position.

Biotechnol Prog 2020 Jan 21:e2967. Epub 2020 Jan 21.

Department of Cell Culture, Genentech, Inc., South San Francisco, California, USA.

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http://dx.doi.org/10.1002/btpr.2967DOI Listing
January 2020

Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express.

Biotechnol Prog 2019 03 10;35(2):e2772. Epub 2019 Jan 10.

Dept. of Cell Culture, Genentech Inc., South San Francisco, California, 94080.

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http://dx.doi.org/10.1002/btpr.2772DOI Listing
March 2019

High Intracellular Seed Train BiP Levels Correlate With Poor Production Culture Performance in CHO Cells.

Biotechnol J 2018 Oct 24;13(10):e1700746. Epub 2018 Apr 24.

Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, 94080, USA.

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http://dx.doi.org/10.1002/biot.201700746DOI Listing
October 2018

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not.

Biotechnol Prog 2018 05 9;34(3):559-569. Epub 2017 Oct 9.

Early Stage Cell Culture (ESCC) Department, Genentech, Inc., 1 DNA Way, South San Francisco, California, 94080.

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http://dx.doi.org/10.1002/btpr.2560DOI Listing
May 2018

Development and characterization of an automated imaging workflow to generate clonally-derived cell lines for therapeutic proteins.

Biotechnol Prog 2018 05 10;34(3):584-592. Epub 2017 Oct 10.

Early Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080.

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http://dx.doi.org/10.1002/btpr.2561DOI Listing
May 2018

Supplementation of Nucleosides During Selection can Reduce Sequence Variant Levels in CHO Cells Using GS/MSX Selection System.

Biotechnol J 2018 Jan 17;13(1). Epub 2017 Aug 17.

Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.

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http://dx.doi.org/10.1002/biot.201700335DOI Listing
January 2018

Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells.

Biotechnol Prog 2017 May 24;33(3):786-794. Epub 2017 Mar 24.

Dept. of Early Stage Cell Culture, Genentech Inc, 1 DNA Way, South San Francisco, CA, 94080.

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http://doi.wiley.com/10.1002/btpr.2444
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http://dx.doi.org/10.1002/btpr.2444DOI Listing
May 2017

Slashing the timelines: Opting to generate high-titer clonal lines faster via viability-based single cell sorting.

Biotechnol Prog 2016 Jan-Feb;32(1):198-207. Epub 2015 Dec 12.

Dept. of Immunology, Genentech, Inc, 1 DNA Way, South San Francisco, CA, 94080.

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http://dx.doi.org/10.1002/btpr.2204DOI Listing
November 2016

It's time to regulate: coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.

Biotechnol Prog 2014 Nov-Dec;30(6):1432-40. Epub 2014 Aug 16.

Dept. of Early Stage Cell Culture, Genentech, Inc, 1 DNA way, South San, Francisco, CA, 94080.

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http://dx.doi.org/10.1002/btpr.1970DOI Listing
August 2015

Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined φC31 integrase and CRE-Lox technologies.

Biotechnol Prog 2013 Sep-Oct;29(5):1307-15. Epub 2013 Sep 3.

Dept. of Early Stage Cell Culture, Genentech Inc., A Member of the Roche Group, South San Francisco, CA, 94080.

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http://dx.doi.org/10.1002/btpr.1783DOI Listing
May 2015

Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production.

Biotechnol Prog 2013 May-Jun;29(3):727-37. Epub 2013 Apr 18.

Dept. of Early Stage Cell Culture, Genentech, Inc., 1 DNA way, South San Francisco, CA, 94080, USA.

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http://dx.doi.org/10.1002/btpr.1722DOI Listing
January 2014

Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells.

Biotechnol Bioeng 2010 Sep;107(1):163-71

Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, California 94080, USA.

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http://dx.doi.org/10.1002/bit.22780DOI Listing
September 2010

Application of dynamic flux balance analysis to an industrial Escherichia coli fermentation.

Metab Eng 2010 Mar 29;12(2):150-60. Epub 2009 Jul 29.

Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.

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http://dx.doi.org/10.1016/j.ymben.2009.07.006DOI Listing
March 2010

High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain.

Biotechnol Bioeng 2004 Mar;85(5):463-74

Department of Cell Culture and Fermentation Research and Development, Genentech, Inc., South San Francisco, California 94080, USA.

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http://doi.wiley.com/10.1002/bit.20014
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http://dx.doi.org/10.1002/bit.20014DOI Listing
March 2004