Publications by authors named "Boyuan Huang"

11 Publications

  • Page 1 of 1

Pro-inflammatory and proliferative microglia drive progression of glioblastoma.

Cell Rep 2021 Sep;36(11):109718

Department of Neurosurgery, The First Medical Center of Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China. Electronic address:

Scant understanding of the glioblastoma microenvironment and molecular bases hampers development of efficient treatment strategies. Analyses of gene signatures of human gliomas demonstrate that the SETD2 mutation is correlated with poor prognosis of IDH1/2 wild-type (IDH-WT) adult glioblastoma patients. To better understand the crosstalk between SETD2 mutant (SETD2-mut) glioblastoma cells and the tumor microenvironment, we leverage single-cell transcriptomics to comprehensively map cellular populations in glioblastoma. In this study, we identify a specific subtype of high-grade glioma-associated microglia (HGG-AM). Further analysis shows that transforming growth factor (TGF)-β1 derived from SETD2-mut/IDH-WT tumor cells activates HGG-AM, exhibiting pro-inflammation and proliferation signatures. Particularly, HGG-AM secretes interleukin (IL)-1β via the apolipoprotein E (ApoE)-mediated NLRP1 inflammasome, thereby promoting tumor progression. HGG-AM present extensive proliferation and infiltration to supplement the activated microglia pool. Notably, TGF-β1/TβRI depletion dramatically reduces HGG-AM density and suppresses tumor growth. Altogether, our studies identify a specific microglia subpopulation and establish the cellular basis of interactions between HGG-AM and glioblastoma cells.
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http://dx.doi.org/10.1016/j.celrep.2021.109718DOI Listing
September 2021

Current Immunotherapies for Glioblastoma Multiforme.

Front Immunol 2020 9;11:603911. Epub 2021 Mar 9.

Department of Neurosurgery, Huizhou Third People's Hospital, Guangzhou Medical University, Huizhou, China.

Glioblastoma multiforme (GBM) is the most common and aggressive malignant tumor found in the central nervous system. Currently, standard treatments in the clinic include maximal safe surgical resection, radiation, and chemotherapy and are mostly limited by low therapeutic efficiency correlated with poor prognosis. Immunotherapy, which predominantly focuses on peptide vaccines, dendritic cell vaccines, chimeric antigen receptor T cells, checkpoint inhibitor therapy, and oncolytic virotherapy, have achieved some promising results in both preclinical and clinical trials. The future of immune therapy for GBM requires an integrated effort with rational combinations of vaccine therapy, cell therapy, and radio- and chemotherapy as well as molecule therapy targeting the tumor microenvironment.
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http://dx.doi.org/10.3389/fimmu.2020.603911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986847PMC
June 2021

High-throughput sequential excitation for nanoscale mapping of electrochemical strain in granular ceria.

Nanoscale 2019 Dec 28;11(48):23188-23196. Epub 2019 Nov 28.

Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA. and Shenzhen Key Laboratory of Nanobiomechanics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China.

Dynamic strain based atomic force microscopy (AFM) modes often fail at the interfaces where the most interesting physics occurs because of their incapability of tracking contact resonance accurately under rough topography. To overcome this difficulty, we develop a high-throughput sequential excitation AFM that captures contact dynamics of probe-sample interactions with high fidelity and efficiency, acquiring the spectrum of data on each pixel over a range of frequencies that are excited in a sequential manner. Using electrochemically active granular ceria as an example, we map both linear and quadratic electrochemical strain accurately across grain boundaries with high spatial resolution where the conventional approach fails. The enhanced electrochemical responses point to the accumulation of small polarons in the space charge region at the grain boundaries, thought to be responsible for the enhanced electronic conductivity in nanocrystalline ceria. The spectrum of data can be processed very efficiently by physics-informed principal component analysis (PCA), speeding data processing by several orders of magnitude. This approach can be applied to a variety of AFM modes for studying a wide range of materials and structures on the nanoscale.
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http://dx.doi.org/10.1039/c9nr07438dDOI Listing
December 2019

Nanoscale Insights into Photovoltaic Hysteresis in Triple-Cation Mixed-Halide Perovskite: Resolving the Role of Polarization and Ionic Migration.

Adv Mater 2019 Sep 19;31(36):e1902870. Epub 2019 Jul 19.

Department of Mechanical Engineering, University of Washington, Seattle, WA, 98195-2600, USA.

Triple-cation mixed-halide perovskites of composition Cs (FA MA ) Pb(I Br ) (CsFAMA) have been reported to possess excellent photovoltaic efficiency with minimal hysteresis; in this work, nanoscale insight is shed into the roles of illumination-induced polarization and ionic migration in photovoltaic hysteresis. By examining the concurrent evolution of ionic distribution and spontaneous polarization of CsFAMA under light illumination using dynamic-strain-based scanning probe microscopy, strong linear piezoelectricity arising from photoenhanced polarization is observed, while ionic migration is found to be not significantly increased by lightening. Nanoscale photocurrents are mapped under a series of biases using conductive atomic force microscopy, revealing negligible difference between forward and backward scans, and local IV curves reconstructed from principal component analysis show minimal hysteresis of just 1%. These observations at the nanoscale are confirmed in a macroscopic perovskite solar cell made of CsFAMA, exhibiting a high efficiency of 20.11% and with hysteresis index as small as 3%. Ionic migration, polarization, and photocurrent hysteresis are thus directly correlated at the nanoscale, and photoenhanced polarization in triple-cation mixed-halide perovskites is established, which does not contribute to the photovoltaic hysteresis.
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http://dx.doi.org/10.1002/adma.201902870DOI Listing
September 2019

Resolving fine electromechanical structure of collagen fibrils via sequential excitation piezoresponse force microscopy.

Nanotechnology 2019 May 30;30(20):205703. Epub 2019 Jan 30.

School of Materials Science and Engineering, Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Xiangtan University, Xiangtan, Hunan 411105, People's Republic of China. Shenzhen Key Laboratory of Nanobiomechanics, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, People's Republic of China.

Collagen is the main protein in extracellular matrix that is found in many connective tissues, and it exhibits piezoelectricity that is expected to correlate with its hierarchical microstructure. Resolving fine electromechanical structure of collagen, however, is challenging, due to its weak piezoresponse, rough topography, and microstructural hierarchy. Here we adopt the newly developed sequential excitation strategy in combination with piezoresponse force microscopy to overcome these difficulties. It excites the local electromechanical response of collagen via a sequence of distinct frequencies, minimizing crosstalk with topography, followed by principal component analysis to remove the background noise and simple harmonic oscillator model for physical analysis and data reconstruction. These enable us to acquire high fidelity mappings of fine electromechanical response at the nanoscale that correlate with the gap and overlap domains of collagen fibrils, which show substantial improvement over conventional piezoresponse force microscopy techniques. It also embodies the spirit of big data atomic force microscopy that can be readily extended into other applications with targeted data acquisition.
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http://dx.doi.org/10.1088/1361-6528/ab0340DOI Listing
May 2019

An artificial intelligence atomic force microscope enabled by machine learning.

Nanoscale 2018 Dec 13;10(45):21320-21326. Epub 2018 Nov 13.

Department of Mechanical Engineering, University of Washington, Seattle, WA 98195-2600, USA.

Artificial intelligence (AI) and machine learning have promised to revolutionize the way we live and work, and one of the particularly promising areas for AI is image analysis. Nevertheless, many current AI applications focus on the post-processing of data, while in both materials sciences and medicine, it is often critical to respond to the data acquired on the fly. Here we demonstrate an artificial intelligence atomic force microscope (AI-AFM) that is capable of not only pattern recognition and feature identification in ferroelectric materials and electrochemical systems, but can also respond to classification via adaptive experimentation with additional probing at critical domain walls and grain boundaries, all in real time on the fly without human interference. Key to our success is a highly efficient machine learning strategy based on a support vector machine (SVM) algorithm capable of high fidelity pixel-by-pixel recognition instead of relying on the data from full mapping, making real time classification and control possible during scanning, with which complex electromechanical couplings at the nanoscale in different material systems can be resolved by AI. For AFM experiments that are often tedious, elusive, and heavily rely on human insight for execution and analysis, this is a major disruption in methodology, and we believe that such a strategy empowered by machine learning is applicable to a wide range of instrumentations and broader physical machineries.
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http://dx.doi.org/10.1039/c8nr06734aDOI Listing
December 2018

High expression level of long non-coding RNA HOTAIR is associated with poor overall survival in gastric cancer patients: evidence from meta-analysis.

J BUON 2017 Jul-Aug;22(4):911-918

Department of Surgical Oncology, Gansu Provincial Hospital, Lanzhou, Gansu, China.

Purpose: Although several studies have investigated the association between the development of gastric cancer (GC) and the expression level of long non-coding (lnc) RNA HOTAIR, no clear evidence about whether its expression is associated with the overall survival (OS) of GC patients exists. In this study we tried to explore the association between lncRNA HOTAIR expression levels with OS and other clinical features in GC patients.

Methods: Databases including PubMed, EMBASE and the Cochrane Library were used to search eligible studies. The quality of included studies was assessed according to reporting recommendations for tumor marker prognostic studies (REMARK). The association between the expression level of lncRNA HOTAIR and OS was evaluated by calculating the pooled hazard ratio (HR) and 95% confidence interval (95% CI) using the STATA software, version 12.0.

Results: A total of 9 studies involving 740 GC and 768 normal gastric tissues were included in this meta-analysis. The average score of quality assessment was 18.89±1.08, (range 16.5-20). The results indicated that high expression levels of lncRNA HOTAIR were associated with poor OS in GC patients (pooled HR: 1.43, 95% CI:1.17-1.76, p=0.000). Subgroup analyses showed that elevated expression of lncRNA HOTAIR was significantly associated with poor OS in Chinese GC patients (HR=1.414, 95%CI: 1.120-1.785, p=0.000), and not treated GC patients (HR=1.464, 95%CI: 1.179-1.817, p=0.001). Subgroup analyses also revealed that some GC patients features (e.g. T3-T4, III/IV stage, differentiation) were associated with an unfavorable outcome.

Conclusions: High expression level of lncRNA HOTAIR is associated with a poor OS in GC patients. Thus, lncRNA HOTAIR might be a potentially useful independent prognostic biomarker for GC.
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July 2019

Microstructure Characterization and Wear-Resistant Properties Evaluation of an Intermetallic Composite in Ni-Mo-Si System.

Materials (Basel) 2017 Feb 4;10(2). Epub 2017 Feb 4.

School of Metallurgy and Energy, North China University of Science and Technology, Tangshan 063009, China.

Intermetallic compounds have been studied for their potential application as structural wear materials or coatings on engineering steels. In the present work, a newly designed intermetallic composite in a Ni-Mo-Si system was fabricated by arc-melting process with commercially pure metal powders as starting materials. The chemical composition of this intermetallic composite is 45Ni-40Mo-15Si (at %), selected according to the ternary alloy diagram. The microstructure was characterized using optical microscopy (OM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS), and the wear-resistant properties at room temperature were evaluated under different wear test conditions. Microstructure characterization showed that the composite has a dense and uniform microstructure. XRD results showed that the intermetallic composite is constituted by a binary intermetallic compound NiMo and a ternary Mo₂Ni₃Si metal silicide phase. Wear test results indicated that the intermetallic composite has an excellent wear-resistance at room-temperature, which is attributed to the high hardness and strong atomic bonding of constituent phases NiMo and Mo₂Ni₃Si.
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http://dx.doi.org/10.3390/ma10020130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459158PMC
February 2017

Advances in Immunotherapy for Glioblastoma Multiforme.

J Immunol Res 2017 19;2017:3597613. Epub 2017 Feb 19.

Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.

Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor in adults. Patients with GBM have poor outcomes, even with the current gold-standard first-line treatment: maximal safe resection combined with radiotherapy and temozolomide chemotherapy. Accumulating evidence suggests that advances in antigen-specific cancer vaccines and immune checkpoint blockade in other advanced tumors may provide an appealing promise for immunotherapy in glioma. The future of therapy for GBM will likely incorporate a combinatorial, personalized approach, including current conventional treatments, active immunotherapeutics, plus agents targeting immunosuppressive checkpoints.
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http://dx.doi.org/10.1155/2017/3597613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337363PMC
April 2017

Central nervous system damage due to acute paraquat poisoning: an experimental study with rat model.

Neurotoxicology 2013 Mar 21;35:62-70. Epub 2012 Dec 21.

Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.

Paraquat (PQ) is a common herbicide and PQ poisoning is a major medical problem in Asia. However, few studies have focused on the acute neurotoxic changes caused by PQ. Here we report the acute neurotoxicological findings of rats treated with lethal dose of PQ. In substantia nigra (SN) and striatum we found obvious microglia (labeled by Iba-1) activation within one week. In SN and hippocampus, we detected increased oxidative stress in the neurons based on NeuN/8-OHdG immunofluorescence double labeling and laser cofocal microscopy. Moreover, we provided ultrastructural evidences of astrocyte edema and neurons apoptosis in rat brain by electron microscopy. Further studies will be needed with non-lethal dose of PQ to confirm these results and demonstrate the direct CNS toxicity of PQ.
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http://dx.doi.org/10.1016/j.neuro.2012.12.001DOI Listing
March 2013

[Investigation of morphology and anatomic variations of circle of Willis and measurement of diameter of cerebral arteries by 3D-TOF angiography].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2007 Feb;24(1):39-44

Department of Medical Imaging, The Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China.

To observe the morphology and anatomic variations of the circle of Willis and its clinical significance. To measure and establish the normal reference parameters of average diameters of its component vessels in the healthy Chinese by using 3D-TOF MR angiograms and to determine whether age-or sex-related differences exists in the circle' anatomy. MRA was performed in 153 healthy subjects. According to its integrity, Willis'circle was divided into four types: (1) Type I, showing an intact circle. (2) Type II, having a complete anterior circulation but an incomplete posterior circulation. (3) Type III, having an incomplete anterior circulation but a complete posterior circulation. (4) Type IV, having an incomplete anterior and posterior circulation. Based on the development of the components of the circle, each type was subdivided into four subtypes: typical, variable, hypoplastic and mixed. The statistically significant differences were not found in each type or subtypes between the different age and sex groups. Result of 153 cases, Type I, II, III and IV was found in 53 (34.64%), 73 (47.71%), 8 (5.23%), and 19 (12.42%) respectively. The display rate of the anterior and the posterior communicating arteries was 87.58%o (134/153) and 65.34% (100/153) respectively. Display rate of fetaltype posterior cerebral artery(PCA) was 20.92%. 3D-TOF MR angiography has important clinical significance for evaluating morphology and anatomic variations of circle of Willis. The normal values of diameter of cerebral arteries on MR angiograms may play a reference role in diagnosing cerebral vascular diseases.
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February 2007
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