Publications by authors named "Bohao Liu"

18 Publications

  • Page 1 of 1

Inflammatory Caspases Drive Pyroptosis in Acute Lung Injury.

Front Pharmacol 2021 5;12:631256. Epub 2021 Feb 5.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Acute lung injury (ALI), a critical respiratory disorder that causes diffuse alveolar injury leads to high mortality rates with no effective treatment. ALI is characterized by varying degrees of ventilation/perfusion mismatch, severe hypoxemia, and poor pulmonary compliance. The diffuse injury to cells is one of most important pathological characteristics of ALI. Pyroptosis is a form of programmed cell death distinguished from apoptosis induced by inflammatory caspases, which can release inflammatory cytokines to clear cells infected by pathogens and promote monocytes to reassemble at the site of injury. And pyroptosis not only promotes inflammation in certain cell types, but also regulates many downstream pathways to perform different functions. There is increasing evidence that pyroptosis and its related inflammatory caspases play an important role in the development of acute lung injury. The main modes of activation of pyroptosis is not consistent among different types of cells in lung tissue. Meanwhile, inhibition of inflammasome, the key to initiating pyroptosis is currently the main way to treat acute lung injury. The review summarizes the relationship among inflammatory caspases, pyroptosis and acute lung injury and provides general directions and strategies to conduct further research.
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http://dx.doi.org/10.3389/fphar.2021.631256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7892432PMC
February 2021

Cell type-specific microRNA therapies for myocardial infarction.

Sci Transl Med 2021 Feb;13(580)

Department of Medicine, Columbia University, New York, NY 10032, USA.

Current interventions fail to recover injured myocardium after infarction and prompt the need for development of cardioprotective strategies. Of increasing interest is the therapeutic use of microRNAs to control gene expression through specific targeting of mRNAs. In this Review, we discuss current microRNA-based therapeutic strategies, describing the outcomes and limitations of key microRNAs with a focus on target cell types and molecular pathways. Last, we offer a perspective on the outlook of microRNA therapies for myocardial infarction, highlighting the outstanding challenges and emerging strategies.
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http://dx.doi.org/10.1126/scitranslmed.abd0914DOI Listing
February 2021

The prognostic value of tumor-stromal ratio combined with TNM staging system in esophagus squamous cell carcinoma.

J Cancer 2021 1;12(4):1105-1114. Epub 2021 Jan 1.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Tumor stroma is a crucial component of the tumor environment that interacted with tumor cells and modulated tumor cell proliferation, immune evasion, and metastasis. Tumor-stromal ratio (TSR) has been confirmed as an influential independent prognostic factor for diverse types of cancer, but it was seldom discussed in esophagus squamous cell carcinoma (ESCC). In present study, pathological sections from the most invasive part of the ESCC of 270 patients were analyzed for their TSR by visual inspection and software. The TSR was combined with the TNM staging system to further explain its predictive value of prognosis. The 57 cases ESCC from TCGA database also were included as an independently validated cohort. Our results indicated that TSR was a robust prognostic factor for ESCC patients. TSR by visual inspection was dependable to reflect the stroma percent of the tumor compared to software calculation. Compared with stroma-low groups, the risk of death increased by 153.1% for patients in the stroma-high group [HR=2.531 (95%CI 1.657-3.867), <0.001]. The results of ROC analysis in two cohorts indicated that TSNM staging system had better resolving ability with the largest area under the curve [0.698 95%CI (0.635-0.760), 0.691 95%CI (0.555-0.807)], compare to TNM. The novel TSNM staging system revealed strong predictive performance (<0.001). TSR was a reliable dependent indicator for ESCC prognosis. The TSNM staging system has a better discriminative ability than the conventional TNM staging system, especially for III stage patients.
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http://dx.doi.org/10.7150/jca.50439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797665PMC
January 2021

MBD2 Mediates Septic AKI through Activation of PKCη/p38MAPK and the ERK1/2 Axis.

Mol Ther Nucleic Acids 2021 Mar 28;23:76-88. Epub 2020 Sep 28.

Department of Emergency Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Our previous study demonstrated that the methyl-CpG-binding domain protein 2 (MBD2) mediates vancomycin (VAN)-induced acute kidney injury (AKI). However, the role and regulation of MBD2 in septic AKI are unknown. Herein, MBD2 was induced by lipopolysaccharide (LPS) in Boston University mouse proximal tubules (BUMPTs) and mice. For both and experiments, we showed that inhibition of MBD2 by MBD2 small interfering RNA (siRNA) and MBD2-knockout (KO) substantially improved the survival rate and attenuated both LPS and cecal ligation and puncture (CLP)-induced AKI, renal cell apoptosis, and inflammatory factor production. Global genetic expression analyses and experiments suggest that the expression of protein kinase C eta (PKCη), caused by LPS, is markedly suppressed in MBD2-KO mice and MBD2 siRNA, respectively. Mechanistically, chromatin immunoprecipitation (ChIP) analysis indicates that MBD2 directly binds to promoter region CpG islands of PKCη via suppression of promoter methylation. Furthermore, PKCη siRNA improves the survival rate and attenuates LPS-induced BUMPT cell apoptosis and inflammatory factor production via inactivation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK)1/2, which were further verified by PKCη siRNA treatment in CLP-induced AKI. Finally, MBD2-KO mice exhibited CLP-induced renal cell apoptosis and inflammatory factor production by inactivation of PKCη/p38MAPK and ERK1/2 signaling. Taken together, the data indicate that MBD2 mediates septic-induced AKI through the activation of PKCη/p38MAPK and the ERK1/2 axis. MBD2 represents a potential target for treatment of septic AKI.
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http://dx.doi.org/10.1016/j.omtn.2020.09.028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723772PMC
March 2021

Extracellular Vesicles in Cardiac Regeneration: Potential Applications for Tissues-on-a-Chip.

Trends Biotechnol 2020 Sep 18. Epub 2020 Sep 18.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Research Institute, University Health Network, Toronto, ON, Canada. Electronic address:

Strategies to regenerate cardiac tissue postinjury are limited and heart transplantation remains the only 'cure' for a failing heart. Extracellular vesicles (EVs), membrane-bound cell secretions important in intercellular signaling, have been shown to play a crucial role in regulating heart function. A mechanistic understanding of the role of EVs in the heart remains elusive due to the challenges in studying the native human heart. Tissue-on-a-chip platforms, comprising functional, physiologically relevant human tissue models, are an emerging technology that has yet to be fully applied to the study of EVs. In this review, we summarize recent advances in cardiac tissue-on-a-chip (CTC) platforms and discuss how they are uniquely situated to advance our understanding of EVs in cardiac disease and regeneration.
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http://dx.doi.org/10.1016/j.tibtech.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969481PMC
September 2020

DsbA-L mediated renal tubulointerstitial fibrosis in UUO mice.

Nat Commun 2020 09 18;11(1):4467. Epub 2020 Sep 18.

Department of Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Recent studies have reported that upregulation of disulfide-bond A oxidoreductase-like protein (DsbA-L) prevented lipid-induced renal injury in diabetic nephropathy (DN). However, the role and regulation of proximal tubular DsbA-L for renal tubulointerstitial fibrosis (TIF) remains unclear. In current study, we found that a proximal tubules-specific DsbA-L knockout mouse (PT-DsbA-L-KO) attenuated UUO-induced TIF, renal cell apoptosis and inflammation. Mechanistically, the DsbA-L interacted with Hsp90 in mitochondria of BUMPT cells which activated the signaling of Smad3 and p53 to produce connective tissue growth factor (CTGF) and then resulted in accumulation of ECM of BUMPT cells and mouse kidney fibroblasts. In addition, the progression of TIF caused by UUO, ischemic/reperfusion (I/R), aristolochic acid, and repeated acute low-dose cisplatin was also alleviated in PT-DsbA-L-KO mice via the activation of Hsp90 /Smad3 and p53/CTGF axis. Finally, the above molecular changes were verified in the kidney biopsies from patients with obstructive nephropathy (Ob). Together, these results suggest that DsbA-L in proximal tubular cells promotes TIF via activation of the Hsp90 /Smad3 and p53/CTGF axis.
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http://dx.doi.org/10.1038/s41467-020-18304-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501299PMC
September 2020

Contributions of Mass Spectrometry-Based Proteomics to Understanding -Host Interactions.

Pathogens 2020 Jul 17;9(7). Epub 2020 Jul 17.

Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

As a model pathogen, invades both phagocytic and non-phagocytic host cells and adopts an intracellular lifestyle in a membrane-bound compartment during infection. Therefore, a systemic overview of adaptations to distinct host cells together with host remodeling will assist us in charting the landscape of host-pathogen interactions. Central to the -host interplay are bacterial virulence factors (effectors) that are injected into host cells by type III secretion systems (T3SSs). Despite great progress, functional studies of bacterial effectors have experienced daunting challenges as well. In the last decade, mass spectrometry-based proteomics has evolved into a powerful technological platform that can quantitatively measure thousands of proteins in terms of their expression as well as post-translational modifications. Here, we will review the applications of high-throughput proteomic technologies in understanding the dynamic reprogramming of both and host proteomes during the course of infection. Furthermore, we will summarize the progress in utilizing affinity purification-mass spectrometry to screen for host substrates of T3SS effectors. Finally, we will critically discuss some limitations/challenges with current proteomic platforms in the context of host-pathogen interactions and highlight some emerging technologies that may offer the promise of tackling these problems.
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http://dx.doi.org/10.3390/pathogens9070581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400052PMC
July 2020

Survival Following Segmentectomy or Lobectomy in Patients With Stage IB Non-small-cell Lung Cancer.

Front Oncol 2020 15;10:661. Epub 2020 May 15.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Lobectomy with mediastinal lymph node dissection has always been recognized as the standardized treatment for early-stage non-small-cell lung cancer. However, the feasibility of segmentectomy performed in stage IB non-small-cell lung cancer (NSCLC) patients remains controversial. The present study aims to investigate whether the outcome of stage IB NSCLC patients undergoing segmentectomy was comparable to those who underwent lobectomy. We retrospectively collected data of 11,010 patients with primary stage IB non-small-cell lung cancer from the Surveillance, Epidemiology, and End Results database. Overall survival (OS) and lung cancer-specific survival (LCSS) were assessed among patients who were performed lobectomy or segmentectomy. To further assess the impact of the surgical procedures on patients with different tumor sizes, subgroups stratified by tumor size were analyzed. A total of 11,010 patients who were pathologically confirmed to be stage IB were included, of whom 10,453 received lobectomy and 557 received segmentectomy. Both univariate and multivariate Cox regression analyses showed that the patients receiving lobectomy had better OS [hazards ratio (HR) = 1.197, 95% confidence interval (CI) (1.066, 1.343), < 0.001] than those receiving segmentectomy. However, multivariate analysis showed that there was no significant difference in LCSS between lobectomy and segmentectomy [HR = 1.172, 95% CI (0.963, 1.427), = 0.114]. Meanwhile, subgroup analyses showed that lobectomy rather than segmentectomy was associated with better OS [HR = 1.278, 95% CI (1.075, 1.520) = 0.006] and LCSS [HR = 1.118, 95% CI (1.005, 1.280), = 0.047] for patients with a tumor size (TS) of ≤ 40 and >30 mm, while for patients with a TS of ≤ 30 mm, lobectomy yielded similar OS [TS ≤ 20 mm: HR = 1.068, 95% CI (0.853, 1.336), = 0.566; TS > 20 mm and ≤ 30 mm: HR = 1.195, 95% CI (0.961, 1.487), = 0.109] and LCSS [TS ≤ 20 mm: HR = 1.029, 95% CI: (0.682, 1.552), = 0.893; TS > 20 and ≤ 30 mm: HR = 1.144, 95% CI (0.795, 1.645), = 0.469] to that of segmentectomy. Segmentectomy achieved equivalent OS and LCSS in stage IB NSCLC patients with TS ≤ 30 mm compared with lobectomy. Lobectomy showed better OS and LCSS than segmentectomy for patients with a TS of >30 and ≤ 40 mm. Segmentectomy may be acceptable in patients with an older age and a smaller TS.
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http://dx.doi.org/10.3389/fonc.2020.00661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243118PMC
May 2020

Facile and low-cost fabrication of a humidity sensor using naturally available sepiolite nanofibers.

Nanotechnology 2020 Aug 14;31(35):355501. Epub 2020 May 14.

State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, People's Republic of China.

Much effort has focussed on enhancing the humidity-sensing performances of humidity sensors, but their fabrication using facile and low-cost methods is also desirable. In this work, a humidity sensor based on a naturally available nanomaterial, sepiolite nanofibers (SNFs), was facilely fabricated without any expensive raw materials or complex processes. Characterization results show that SNFs have a natural slender nanofiber structure (diameter 20-50 nm) and abundant hydrophilic functional groups (-OH). The results of humidity-sensing tests show that the SNF humidity sensor has outstanding humidity-sensing properties (i.e. large response, good linearity and repeatability) within the relative humidity range from 10.9% to 91.5% at room temperature (25 °C). This work presents a moderate and cost-effective strategy for the fabrication of high-performance humidity sensors using the natural SNF nanomaterial.
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http://dx.doi.org/10.1088/1361-6528/ab932cDOI Listing
August 2020

The Biomarker TCONS_00016233 Drives Septic AKI by Targeting the miR-22-3p/AIFM1 Signaling Axis.

Mol Ther Nucleic Acids 2020 Mar 14;19:1027-1042. Epub 2020 Jan 14.

Department of Emergency Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China; Emergency Medicine and Difficult Diseases Institute, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. Electronic address:

The prediction of mortality for septic acute kidney injury (AKI) has been assessed by a number of potential biomarkers, including long noncoding RNAs (lncRNAs). However, the validation of lncRNAs as biomarkers, particularly for the early stages of septic AKI, is still warranted. Our results indicate that the lncRNA TCONS_00016233 is upregulated in plasma of sepsis-associated non-AKI and AKI patients, but a higher cutoff threshold (9.5 × 10, copy number) provided a sensitivity of 71.9% and specificity of 89.6% for the detection of AKI. The plasma TCONS_00016233 was highly correlated with serum creatinine, tissue inhibitor metalloproteinase-2 (TIMP-2), insulin-like growth factor binding protein-7 (IGFBP7), interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), C-reactive protein (CRP), and urinary TCONS_00016233. Lipopolysaccharide (LPS) induced the expression of lncRNA TCONS_00016233 via the Toll-like receptor 4 (TLR4)/p38 mitogen-activated protein kinase (MAPK) signal pathway in human renal tubular epithelial (HK-2) cells. Furthermore, TCONS_00016233 mediates the LPS-induced HK-2 cell apoptosis and the expression of IL-1β and TNF-α. Mechanistically, TCONS_00016233 acts as a competing endogenous RNA (ceRNA) to prevent microRNA (miR)-22-3p-mediated downregulation of the apoptosis-inducing factor mitochondrion-associated 1 (AIFM1). Finally, overexpression of TCONS_00016233 is capable of aggravating the LPS- and cecal ligation and puncture (CLP)-induced septic AKI by targeting the miR-22-3p/AIFM1 axis. Taken together, our data indicate that TCONS_00016233 may serve as an early diagnosis marker for the septic AKI, possibly acting as a novel therapeutic target for septic AKI.
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http://dx.doi.org/10.1016/j.omtn.2019.12.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016165PMC
March 2020

Characterizing pump line phase offset of a single-soliton Kerr comb by dual comb interferometry.

Opt Lett 2019 Mar;44(6):1460-1463

We report phase retrieval of a single-soliton Kerr comb using electric field cross-correlation implemented via dual-comb interferometry. The phase profile of the Kerr comb is acquired through the heterodyne beat between the Kerr comb and an electro-optic comb with a pre-characterized phase profile. The soliton Kerr comb has a nearly flat phase profile, and the pump line is observed to show a phase offset which depends on the pumping parameters. The experimental results are in agreement with numerical simulations.
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http://dx.doi.org/10.1364/OL.44.001460DOI Listing
March 2019

Retraction: Differential expression of exosomal miRNAs in osteoblasts in osteoarthritis [Journal of Central South University. Medical Science, 2018, 43(12):1294-1300. DOI: 10.11817/j.issn.1672-7347.2018.12.003].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2019 02;44(2):149

The article entitled "Differential expression of exosomal miRNAs in osteoblasts in osteoarthritis" published on Journal of Central South University (Medical Science), in Volume 43, Issue 12, 2018 (DOI: 10.11817/j.issn.1672-7347.2018.12.003) may have an unclear risk of bias due to insufficient understanding for some results. Further experimental studies are needed. We all agree to retract this article, and apologize to the Journal and readers for the possible negative impact.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2019.02.017DOI Listing
February 2019

Pro-resolving lipid mediator ameliorates obesity induced osteoarthritis by regulating synovial macrophage polarisation.

Sci Rep 2019 01 23;9(1):426. Epub 2019 Jan 23.

Institute of Health and Biomedical Innovation, Faculty of Science and Engineering, Queensland University of Technology, Brisbane, 4059, Australia.

Non-resolved persistent macrophage-mediated synovial inflammation is considered as one of the main drivers of both the establishment and progression of obesity-associated osteoarthritis (OA). Herein, we used clodronate-loaded liposomes (CL) to locally deplete macrophages in the synovial joints to examine the role of macrophages in the progression of obesity-induced OA. Furthermore, resolvin D1 (RvD1), a unique family of pro-resolving lipid mediator derived from the omega-3 polyunsaturated fatty acid, have shown marked potency in changing the pro-inflammatory behaviour of the macrophages. We sought to determine whether RvD1 administration ameliorates obesity-induced OA by resolving macrophage-mediated synovitis. Therapeutic properties of RvD1 and macrophage depletion (CL) were tested for its ability to slow post-traumatic OA (PTOA) in obese mice models. PTOA was induced in C57Bl/6 mice fed with high-fat diet (HFD) by surgically destabilising the meniscus. Firstly, CL treatment showed beneficial effects in reducing synovitis and cartilage destruction in obese mice with PTOA. In vitro treatment with RvD1 decreased the levels of pro-inflammatory markers in CD14+ human macrophages. Furthermore, intra-articular treatment with RvD1 diminishes the progression of OA in the knee joint from mice as follows: (a) decreases macrophages infiltration in synovium, (b) reduces the number of pro-inflammatory macrophages in synovium and (c) improves the severity of synovitis and cartilage degradation. Thus, our results provide new evidence for the potential targeting of macrophages in the treatment of obesity-induced OA.
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http://dx.doi.org/10.1038/s41598-018-36909-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344566PMC
January 2019

[Differential expression of exosomal miRNAs in osteoblasts in osteoarthritis].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2018 Dec;43(12):1294-1300

Department of Orthopedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Objective: To analyze the differentially expressed exosomal miRNAs in subchondral osteoblasts in patients with osteoarthritis (OA) and to investigate the key miRNAs potentially involved in the occurrence and progression of OA.
 Methods: Subchondral bones were harvested from 6 patients with OA. All subjects were divided into two groups which was based on the severity of joint wear: An OA group, severely worn side of subchondral bone, and a control group, less worn side of subchondral bone. The exosomes were extracted from osteoblast cells and their characteristics were identified. Then exosomal miRNAs were extracted and sequencing analysis was conducted to compare the expression in the two groups. The most differentially expressed ones (log2Ratio≥2) were subject to miRNA target prediction and quantitative reverse transcription PCR (RT-qPCR) to further quantify the difference.
 Results: Osteoblast extractions were confirmed to be exosomes, which were small double-membranous vesicles with 30-200 nm in diameter and 50-150 nm in peak value of particle size under the scanning microscope. High-throughput sequencing revealed 124 miRNAs whose expression significantly increased in the OA group. The most differentially expressed one with maximum fold change was hsa-miR-4717-5p and its target gene was RGS2. RT-qPCR demonstrated hsa-miR-4717-5p expression in the OA group was relatively higher than that in the control group (2.243 vs 0.480, P<0.01).
 Conclusion: There is distinct difference in expression profiles of exosomal miRNAs in subchondral osteoblasts between patients with OA and normal subjects. Up-regulated expression of miRANs might participate in OA occurrance and progression.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2018.12.003DOI Listing
December 2018

Space-time focusing in a highly multimode fiber via optical pulse shaping.

Opt Lett 2018 Oct;43(19):4675-4678

Fields propagating through a highly scattering material will be distorted in both space (intensity speckles) and time (spectral and temporal speckles), inhibiting tasks such as imaging and communication in both the optical and radio frequency regions. In optics, research thus far has demonstrated spatial focusing, image transmission, and short pulse delivery through bulk scattering materials and multimode fibers by taking advantage of spatial wavefront-shaping techniques. Here, we exploit spectral phase shaping for reference-free characterization of spectral and temporal speckle, and space-time focusing of broadband ultrafast pulses distorted by modal dispersion in a multimode fiber. We show that temporal speckle fields at different multimode fiber output locations are uncorrelated and demonstrate the ability to focus a short pulse at a specific output spatial location, while keeping the field at other output locations noise-like, offering opportunities to expand multimode fiber imaging and communication capacity.
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http://dx.doi.org/10.1364/OL.43.004675DOI Listing
October 2018

Cardiac recovery via extended cell-free delivery of extracellular vesicles secreted by cardiomyocytes derived from induced pluripotent stem cells.

Nat Biomed Eng 2018 May 23;2(5):293-303. Epub 2018 Apr 23.

Department of Medicine, Columbia University, New York, NY, USA.

The ability of extracellular vesicles (EVs) to regulate a broad range of cellular processes has recently been exploited for the treatment of diseases. For example, EVs secreted by stem cells injected into infarcted hearts can induce recovery through the delivery of stem-cell-specific miRNAs. However, the retention of the EVs and the therapeutic effects are short-lived. Here, we show that an engineered hydrogel patch capable of slowly releasing EVs secreted from cardiomyocytes derived from induced pluripotent stem (iPS) cells reduced arrhythmic burden, promoted ejection-fraction recovery, decreased cardiomyocyte apoptosis 24 hours after infarction, and reduced infarct size and cell hypertrophy 4 weeks post-infarction when implanted onto infarcted rat hearts. We also show that the EVs are enriched with cardiac-specific miRNAs known to modulate cardiomyocyte-specific processes. The extended delivery of EVs secreted from iPS-cell-derived cardiomyocytes into the heart may help understand heart recovery and treat heart injury.
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http://dx.doi.org/10.1038/s41551-018-0229-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159913PMC
May 2018

Modular Assembly Approach to Engineer Geometrically Precise Cardiovascular Tissue.

Adv Healthc Mater 2016 Apr 10;5(8):900-6. Epub 2016 Feb 10.

Laboratory for Stem Cells and Tissue Engineering, Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.

This modular assembly approach to microfabricate functional cardiovascular tissue composites enables quantitative assessment of the effects of microarchitecture on cellular function. Cardiac and endothelial modules are micromolded separately, designed to direct cardiomyocyte alignment and anisotropic contraction or vascular network formation. Assembled cardiovascular tissue composites contract synchronously, facilitating the use of this tissue-engineering platform to study structure-function relationships in the heart.
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http://dx.doi.org/10.1002/adhm.201500956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836958PMC
April 2016