Publications by authors named "Bo Zhu"

1,026 Publications

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Dexmedetomidine Attenuates Total Body Radiation-Induced Acute Liver Injury in Mice Through the Nrf2/HO-1 Pathway.

Clin Lab 2022 Aug;68(8)

Background: The purpose of this study was to investigate the protective effects of dexmedetomidine (DEX) on total body radiation-induced acute liver injury in mice and to explore the possible mechanisms.

Methods: A total of 40 mice were randomly divided into the Control group (Group C), Dexmedetomidine group (Group Dex), Radiation group (Group R), and Group R+Dex. Mice in Group Dex and Group R+Dex were intraperitoneally injected with 10 µg/mL Dex at 50 mg/kg. Both Group C and Group R received normal saline instead of Dex. Mice were treated via continuous administration for 10 days and injection once a day (pre-administration for 3 days and 7 days after radiation). One hour after administration on the third day, the mice in Group R and R+Dex received total body radiation with a total dose of 6 Gy at a rate of 2 Gy/min. Group C received sham radiation. Levels of aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), and liver levels of tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA) were measured. HE staining was employed to evaluate the pathological changes in liver tissues, and the expressions of Nrf2 and HO-1 proteins in the liver were measured by western blot.

Results: Compared with group C, serum levels of AST and ALT, liver TNF-α, IL-1β, MDA, and ROS levels increased, and SOD decreased in Group R. Group R mice had higher liver injury scores, and the protein expressions of Nrf2 and HO-1 proteins were lower (p < 0.05). Compared with Group R, the levels of AST, ALT, TNF-α, IL-1β, MDA, and ROS decreased, SOD increased, liver injury scores were lower, and the expressions of Nrf2 and HO-1 proteins were higher in the Group R+Dex group (all p < 0.05).

Conclusions: Dex exhibits a protective effect on reducing acute radiation-induced liver injury and oxidative stress, and the mechanism may be associated with the activation of Nrf2/HO-1 pathways.
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http://dx.doi.org/10.7754/Clin.Lab.2022.220310DOI Listing
August 2022

Comparison of efficacy and safety of hybrid video-assisted thoracoscopic surgery . thoracotomy sleeve lobectomy for non-small cell lung cancer: a propensity score matching study.

J Thorac Dis 2022 Jul;14(7):2635-2644

Department of Thoracic Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.

Background: It is unclear whether hybrid video-assisted thoracoscopic surgery (VATS) sleeve lobectomy (SL) could be an alternative to thoracotomy SL for non-small cell lung cancer (NSCLC) treatment. The aim of the study is to investigate the safety and efficacy of hybrid VATS SL.

Methods: We collected 112 patients who received hybrid VATS SL or thoracotomy SL for primary treatment of NSCLC at Liaoning Cancer Hospital & Institute from November 2016 to September 2021. Perioperative and survival data were collected, and retrospective analysis was conducted on 91 thoracotomy and 21 hybrid VATS SL patients to evaluate the safety and efficacy of two approaches. Propensity score matching (PSM) was used to minimize selection bias. The Kaplan-Meier method was applied to compare overall survival (OS) and recurrence-free survival (RFS). Follow-up data were obtained by outpatient clinical visit and nurse-led telephone.

Results: A total of 112 patients were included in this study, including thoracotomy patients (n=91) and hybrid VATS patients (n=21). In the non-match analysis, the baseline data of patients was similar between the two groups, except regarding the receipt of neoadjuvant therapy (P=0.087). After PSM (2:1), a total of 39 thoracotomy patients and 19 hybrid VATS patients were enrolled. The baseline variables were quite similar in both groups. In the hybrid group, the number of lymph nodes (25.9±8.5 32.9±9.7, P<0.01) and positive lymph nodes (3.7±2.9 5.6±4.0, P=0.045) were significantly higher than those in the thoracotomy group. The hybrid group was associated with significantly shorter postoperative hospital stay (9.5±3.5 7.3±2.9, P=0.021) and chest tube duration time (6.6±3.1 5.3±1.5, P=0.031). No statistical difference in complications, reoperation, and recurrence were observed between the hybrid VATS SL and thoracotomy SL (P=1.0, P=1.0, P=0.472). The 30- and 90-day mortalities showed no differences between approaches (2.6% 0%, P=1.0; 5.1% 5.3%, P=1.0). No significant differences were found between thoracotomy and hybrid VATS SL in 3-year OS (P=0.614) and 3-year RFS (P=0.750).

Conclusions: Hybrid VATS SL lobectomy may be a safe and feasible approach associated with similar oncologic prognosis and better postoperative recovery compared with thoracotomy SL for NSCLC treatment.
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http://dx.doi.org/10.21037/jtd-22-754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344417PMC
July 2022

Comparison of Affected-Vertebra Fixation of Cortical Bone Trajectory Screw and Pedicle Screw for Lumbar Tuberculosis: A Minimum 3-Year Follow-Up.

Biomed Res Int 2022 21;2022:6312994. Epub 2022 Jul 21.

Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Purpose: This study is aimed at comparing the clinical efficacy of cortical bone trajectory (CBT) screw fixation and pedicle screw (PS) fixation of the affected vertebrae in lumbar tuberculosis.

Methods: We retrospectively analyzed the outcomes in 52 patients (27 cases in the CBT group, 25 cases in the PS group) with lumbar TB who underwent posterior affected-vertebra fixation combined with anterior debridement and bone grafting. The intraoperative blood loss, operative time, visual analog scale (VAS) scores for incision pain and leg pain, Japanese Orthopedic Association (JOA) score, bone grafting fusion, and complications were recorded.

Results: All patients were followed up for 35-52 months and achieved good clinical outcomes. There were no differences between the two groups in the operative time, intraoperative blood loss, JOA score, bone grafting fusion, and complications. However, there was a significant difference between the two groups in VAS scores for incision pain on the 1st day and 3rd day after surgery. At the last follow-up, JOA scores were significantly improved in both groups compared to the preoperation.

Conclusion: This retrospective study confirmed that both the affected-vertebra CBT screw fixation and PS fixation for lumbar TB via posterior and anterior approaches could achieve satisfactory outcomes, while the former resulted in better improvement for postoperative VAS scores.
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http://dx.doi.org/10.1155/2022/6312994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334104PMC
August 2022

Synthesis and Properties of Magnetic FeO/PCL Porous Biocomposite Scaffolds with Different Sizes and Quantities of FeO Particles.

Bioengineering (Basel) 2022 Jun 26;9(7). Epub 2022 Jun 26.

Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA.

In clinical practice, to treat diseases such as osteosarcoma or chondrosarcoma with broad surgical ostectomy, it would be ideal to have scaffolds that not only fill up the bone void but also possess the ability to regulate the subsequent regimes for targeted chemotherapy and/or bone regeneration. Magnetic targeting of therapeutic agents to specific sites in the body provides certain advantages such as minimal side-effects of anti-cancer drugs. The objective of this study was to characterize novel magnetic scaffolds that can be used as a central station to regulate the drug delivery of a magnetic nanoparticle system. Different sizes and quantities of FeO particles were mixed with poly-ε-caprolactone (PCL) to construct the magnetic scaffolds, and their mechanical properties, degradation performance, and cell biocompatibility were evaluated. It appeared that the presence of FeO particles influenced the magnetic, mechanical, and biological performances of the scaffolds. The prepared bio-nanocomposite scaffolds provided predominantly magnetic/superparamagnetic properties. Scaffolds with a micron-sized FeO to PCL weight (wt) ratio of 0.1:0.9 exhibited higher mechanical performances among samples, with Young's modulus reaching 1 MPa and stiffness, 13 N/mm. Although an increased FeO particle proportion mildly influenced cell growth during the biocompatibility test, none of the FeO/PCL scaffolds showed a cytotoxic effect.
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http://dx.doi.org/10.3390/bioengineering9070278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311534PMC
June 2022

Creating a Thermostable β-Glucuronidase Switch for Homogeneous Immunoassay by Disruption of Conserved Salt Bridges at Diagonal Interfaces.

Biochemistry 2022 Jul 18. Epub 2022 Jul 18.

Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.

β-glucuronidase (GUS) has been used as a reporter enzyme in molecular biology and engineered as an enzyme switch for the development of homogeneous biosensors. In this study, we developed a thermostable GUS enzyme switch based on the thermostable GUS mutant TR3337 by disrupting a conserved salt bridge (H514-E523) between the diagonal subunits of its homotetramer. A combinatorial library (240 variants) was screened using a novel high-throughput strategy, which led to the identification of mutant DLW (H514D/M516L/Y517W) as a functional enzyme switch in a caffeine-recognizing immunosensor. Molecular dynamics simulations were performed to predict the topology change around position 514, and a side-chain flip of D514 (repulsion with E523) was observed in the DLW mutant. Up to 1.8-fold of signal-to-background ratio was confirmed when measured at up to 45 °C, thereby highlighting the DLW mutant as a versatile tool for developing thermostable immunosensors for and applications.
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http://dx.doi.org/10.1021/acs.biochem.2c00165DOI Listing
July 2022

Neoantigens in precision cancer immunotherapy: from identification to clinical applications.

Chin Med J (Engl) 2022 Jul 15. Epub 2022 Jul 15.

Institute of Cancer, Xinqiao Hospital, Army Medical University, Chongqing 400037, China.

Abstract: Immunotherapies targeting cancer neoantigens are safe, effective, and precise. Neoantigens can be identified mainly by genomic techniques such as next-generation sequencing and high-throughput single-cell sequencing; proteomic techniques such as mass spectrometry; and bioinformatics tools based on high-throughput sequencing data, mass spectrometry data, and biological databases. Neoantigen-related therapies are widely used in clinical practice and include neoantigen vaccines, neoantigen-specific CD8+ and CD4+ T cells, and neoantigen-pulsed dendritic cells. In addition, neoantigens can be used as biomarkers to assess immunotherapy response, resistance, and prognosis. Therapies based on neoantigens are an important and promising branch of cancer immunotherapy. Unremitting efforts are needed to unravel the comprehensive role of neoantigens in anti-tumor immunity and to extend their clinical application. This review aimed to summarize the progress in neoantigen research and to discuss its opportunities and challenges in precision cancer immunotherapy.
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http://dx.doi.org/10.1097/CM9.0000000000002181DOI Listing
July 2022

Enhancer of zeste homolog 2 exerts functions in gastric cancer development via modulating microRNA-222-3p methylation and WEE1 expression.

Chem Biol Drug Des 2022 Sep 17;100(3):320-333. Epub 2022 Jul 17.

Department of Gastroenterology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.

Enhancer of zeste homolog 2 (EZH2) has been studied in gastric cancer (GC), while the role of EZH2 in GC via binding to microRNA (miR)-222-3p remains obscure. This research aims to unravel the regulatory mechanism of EZH2 in GC progression via the modulation of miR-222-3p/WEE1 axis. Initially, EZH2, miR-222-3p, and WEE1 levels in GC cells and tissues were examined. Thereafter, constructs altering EZH2, miR-222-3p, or WEE1 expression were transfected into GC cells to determine the malignant behaviors involved in tumorigenesis of GC cells. Finally, the targeting relations among EZH2, miR-222-3p, and WEE1 were validated. EZH2 and WEE1 were upregulated while miR-222-3p was down-regulated in GC tissues and cells. The decreased EZH2, silenced WEE1, or restored miR-222-3p constrained the malignant behaviors involved in tumorigenesis of GC cells. Deletion of miR-222-3p could reverse the effect of silenced EZH2 on suppressing the biological functions of GC cells. EZH2 could bind to the promoter of miR-222-3p, and there was a targeting relationship between miR-222-3p and WEE1. Our study demonstrates that EZH2 promotes GC development via the modulation of miR-222-3p/WEE1 axis, thus providing promising therapeutic targets for GC therapy.
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http://dx.doi.org/10.1111/cbdd.14110DOI Listing
September 2022

Switchable Adhesion on Curved Surfaces Mimicking the Coordination of Radial-Oriented Spatular Tips and Motion of Gecko Toes.

ACS Appl Mater Interfaces 2022 Jul 28;14(27):31448-31454. Epub 2022 Jun 28.

School of Power and Mechanical Engineering, The Institute of Technological Science, Wuhan University, Wuhan 430072, China.

Bio-inspired structured adhesives have promising applications in many fields, like biomedicine, robotics, and aerospace. However, achieving robust and switchable adhesion in structured adhesives on non-planar surfaces remains highly challenging. Inspired by the gripping and rolling motions of gecko toes, a strong and switchable adhesive, which comprises a pillar array with radial-oriented spatular tips and is named as PROST, is developed. PROST possesses a robust adhesion on flat surfaces and doubles its adhesion on curved surfaces. Moreover, in situ and fast adhesion switching of PROST on flat/curved surfaces in dry and wet conditions has been realized by solvent stimulation, mimicking the bending locomotion of gecko toes. The work here provides a new strategy for designing controllable adhesion on curved substrates.
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http://dx.doi.org/10.1021/acsami.2c07909DOI Listing
July 2022

MOF-derived nitrogen-doped carbon-based trimetallic bifunctional catalysts for rechargeable zinc-air batteries.

Nanotechnology 2022 Jul 14;33(40). Epub 2022 Jul 14.

State Key Laboratory for Reliability and Intelligence of Electrical Equipment, Hebei Key Laboratory of Smart Sensing and Human-Robot Interaction, School of Mechanical Engineering, Hebei University of Technology, Tianjin 300130, People's Republic of China.

Zinc-air battery (ZAB) is a promising new metal-air energy system, but the large overpotentials of oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) around the air electrode lead to their poor energy efficiency. Herein, a novel bifunctional oxygen electrocatalyst is reported with the preparation of a zeolite imidazolate framework (ZIF-67) derived trimetallic composites decorated nitrogen-doped carbon, which consist of NiFe alloy and Co nanoparticles. The ZIF-derived porous N-doped carbon shell can speed up the mass transfer efficiency. Whereas the electronic effect between the formed NiFe alloy and Co nanoparticles, as well as the N-doped carbon framework can enrich the active centers and enhance the electrical conductivity. As a result, the [email protected] catalyst shows superior performance manifested as a small potential gap (Δ = 0.857 V) between the overpotential at 10 mA cm() for OER (460 mV) and half-wave potential () for ORR (0.833 V). The liquid ZABs exhibit a high specific capacity reaching 798 mAh/gand a stable cycling performance at 10 mA cmfor more than 200 h. Meanwhile, the [email protected] based flexible ZABs also presents robust flexibility and stability. This study has certain implications for the development of economical, powerful and stable bifunctional catalysts for ZABs.
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http://dx.doi.org/10.1088/1361-6528/ac7b98DOI Listing
July 2022

Development and Validation of a Novel Prognostic Model Predicting the Atrial Fibrillation Recurrence Risk for Persistent Atrial Fibrillation Patients Treated with Nifekalant During the First Radiofrequency Catheter Ablation.

Cardiovasc Drugs Ther 2022 Jun 22. Epub 2022 Jun 22.

Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, People's Republic of China.

Background: This study aimed to establish and assess a prediction model for patients with persistent atrial fibrillation (AF) treated with nifekalant during the first radiofrequency catheter ablation (RFCA).

Methods: In this study, 244 patients with persistent AF from January 17, 2017 to December 14, 2017, formed the derivation cohort, and 205 patients with persistent AF from December 15, 2017 to October 28, 2018, constituted the validation cohort. The least absolute shrinkage and selection operator regression was used for variable screening and the multivariable Cox survival model for nomogram development. The accuracy and discriminative capability of this predictive model were assessed according to discrimination (area under the curve [AUC]) and calibration. Clinical practical value was evaluated using decision curve analysis.

Results: Body mass index, AF duration, sex, left atrial diameter, and the different responses after nifekalant administration were identified as AF recurrence-associated factors, all of which were selected for the nomogram. In the development and validation cohorts, the AUC for predicting 1-year AF-free survival was 0.863 (95% confidence interval (CI) 0.801-0.926) and 0.855 (95% CI 0.782-0.929), respectively. The calibration curves showed satisfactory agreement between the actual AF-free survival and the nomogram prediction in the derivation and validation cohorts. In both groups, the prognostic score enabled stratifying the patients into different AF recurrence risk groups.

Conclusions: This predictive nomogram can serve as a quantitative tool for estimating the 1-year AF recurrence risk for patients with persistent AF treated with nifekalant during the first RFCA.
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http://dx.doi.org/10.1007/s10557-022-07353-9DOI Listing
June 2022

Ir/Ni-Metallaphotoredox-Catalyzed Enantioselective Decarboxylative Arylation of α-Amino Acids: Theoretical Insight of Enantio-Determining Outer-Sphere Reductive Elimination.

Inorg Chem 2022 Jul 21;61(26):10190-10197. Epub 2022 Jun 21.

Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University, Changchun 130024, People's Republic of China.

The Ir/Ni-metallaphotoredox-catalyzed enantioselective decarboxylative arylation of α-amino acids has been systematically investigated using density functional theory calculations. The combination of oxidative quenching (Ir-*Ir-Ir-Ir) or reductive quenching (Ir-*Ir-Ir-Ir) cycle with the nickel catalytic cycle (Ni-Ni-Ni-Ni) is possible. The favorable reaction mechanism consists of three major processes: single-electron transfer, oxidative addition, and stepwise outer-sphere reductive elimination. The rate-determining step is the oxidative addition. Unexpectedly, the enantio-determining C-C bond formation occurs via an ion-pair intermediate involved in the stepwise outer-sphere reductive elimination process, which is unusual in the Ir/Ni-metallaphotoredox catalysis. Furthermore, computational results reveal that the high enantioselectivity of this reaction is mainly dependent on the steric effect of substituents on substrates. This theoretical study provides useful knowledge for deep insights into the activity and selectivity of visible-light-mediated enantioselective metallaphotoredox dual catalysis at the molecular and atomic levels and benefits the development of asymmetric synthesis.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01387DOI Listing
July 2022

Genome-wide identification of R2R3-MYB gene family and association with anthocyanin biosynthesis in Brassica species.

BMC Genomics 2022 Jun 14;23(1):441. Epub 2022 Jun 14.

College of Life Sciences, Ganzhou Key Laboratory of Greenhouse Vegetable, Gannan Normal University, 341000, Ganzhou, China.

Brassica species include important oil crops and vegetables in the world. The R2R3-MYB gene participates in a variety of plant functions, including the activation or inhibition of anthocyanin biosynthesis. Although previous studies have reported its phylogenetic relationships, gene structures, and expression patterns in Arabidopsis, the number and sequence variation of this gene family in Brassica crops and its involvement in the natural quantitative variation in anthocyanin biosynthesis regulation are still largely unknown. In this study, by using whole genome sequences and comprehensive genome-wide comparative analysis among the six cultivated Brassica species, 2120 R2R3-MYB genes were identified in six Brassica species, in total These R2R3-MYB genes were phylogenetically clustered into 12 groups. The R2R3-MYB family between A and C subgenomes showed better collinearity than between B and C and between A and B. From comparing transcriptional changes of five Brassica species with the purple and green leaves for the detection of the R2R3-MYB genes associated with anthocyanin biosynthesis, 7 R2R3-MYB genes were co-differentially expressed. The promoter and structure analysis of these genes showed that some variations between non-coding region, but they were highly conserved at the protein level and spatial structure. Co-expression analysis of anthocyanin-related genes and R2R3-MYBs indicated that MYB90 was strongly co-expressed with TT8, and they were co-expressed with structural genes F3H, LDOX, ANS and UF3GT at the same time. These results further clarified the roles of the R2R3-MYBs for leaf coloration in Brasica species, which provided new insights into the functions of the R2R3-MYB gene family in Brasica species.
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http://dx.doi.org/10.1186/s12864-022-08666-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9199147PMC
June 2022

[Real-time Composition and Sources of VOCs in Summer in Wuhan].

Huan Jing Ke Xue 2022 Jun;43(6):2966-2978

School of Environmental Studies, China University of Geosciences, Wuhan 430078, China.

The hourly concentrations of 102 volatile organic compounds (VOCs) in Wuhan from June to July in 2019 were obtained using an online monitoring instrument. The (VOCs) varied from 24.9 to 254 μg·m, with a mean value of (67.7±32.2) μg·m. According to the air quality standard of ozone, the observation period was divided into clean and polluted episodes of O. The differences in meteorological parameters, VOC concentrations, compositions, sources, and ozone formation potential (OFP) between clean and polluted episodes were analyzed and compared. The average mass concentrations of NO, CO, and VOCs in polluted periods exceeded those of clean periods by 34.9%, 25.0%, and 27.8%, respectively. The mass concentrations of alkanes, alkenes, aromatic hydrocarbons, and oxygenated volatile organic compounds in polluted periods were higher than those in clean periods by 40.7%, 39.5%, 26.9%, and 21.5%, respectively. The average OFP in polluted periods[(102±69.6) μg·m] exceeded that of clean periods by 33.5%. The average contribution rates of LPG combustion, industrial sources, vehicle emissions, natural sources, and solvent usage to VOCs were 3.4%, 2.5%, 0.2%, 1.3%, and 1.4% lower than those of the clean periods, respectively, whereas the gasoline evaporation increased by 8.8% in polluted periods. The contributions of vehicle emissions and gasoline evaporation exhibited higher values in the morning and evening, with lower values in the afternoon, which may have been related to peak vehicles emissions. The contribution of LPG combustion peaked along with the cooking time. The concentration weighted trajectory showed that the main sources of VOCs in polluted periods were from local emissions and surrounding regions in the northeastern direction of Wuhan. In polluted periods, gasoline evaporation and LPG combustion should be emphasized for preventing O pollution in the summer in Wuhan.
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http://dx.doi.org/10.13227/j.hjkx.202109027DOI Listing
June 2022

The Role of Endothelial-to-Mesenchymal Transition in Cardiovascular Disease.

Cells 2022 06 3;11(11). Epub 2022 Jun 3.

Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Endothelial-to-mesenchymal transition (EndoMT) is the process of endothelial cells progressively losing endothelial-specific markers and gaining mesenchymal phenotypes. In the normal physiological condition, EndoMT plays a fundamental role in forming the cardiac valves of the developing heart. However, EndoMT contributes to the development of various cardiovascular diseases (CVD), such as atherosclerosis, valve diseases, fibrosis, and pulmonary arterial hypertension (PAH). Therefore, a deeper understanding of the cellular and molecular mechanisms underlying EndoMT in CVD should provide urgently needed insights into reversing this condition. This review summarizes a 30-year span of relevant literature, delineating the EndoMT process in particular, key signaling pathways, and the underlying regulatory networks involved in CVD.
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http://dx.doi.org/10.3390/cells11111834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180466PMC
June 2022

Nasal Spray of Neutralizing Monoclonal Antibody 35B5 Confers Potential Prophylaxis Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern (VOCs): A Small-scale Clinical Trial.

Clin Infect Dis 2022 Jun 6. Epub 2022 Jun 6.

Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, China.

Background: SARS-CoV-2 VOCs, especially the Delta and Omicron variants, have been reported to show significant resistance to approved neutralizing monoclonal antibodies (mAbs) and vaccines. We previously identified a mAb named 35B5 that harbors broad neutralization to SARS-CoV-2 VOCs. Herein, we explored the protection efficacy of a 35B5-based nasal spray against SARS-CoV-2 VOCs in a small-scale clinical trial.

Methods: We enrolled 30 healthy volunteers who were nasally administrated with the modified 35B5 formulation. At 12, 24, 48 and 72 hours after nasal spray, the neutralization efficacy of nasal mucosal samples was assayed with pseudoviruses coated with SARS-CoV-2 Spike protein of the wild-type (WT), Alpha, Beta, Delta, or Omicron variants.

Results: The nasal mucosal samples collected within 24 hours after nasal spray effectively neutralized SARS-CoV-2 VOCs (including Delta and Omicron). Meanwhile, the protection efficacy was 60% effective and 20% effective at 48 and 72 hours after nasal spray, respectively.

Conclusions: A single nasal spray of 35B5 formation conveys 24-hour effective protection against SARS-CoV-2 VOCs, including the Alpha, Beta, Delta, or Omicron variants. Thus, 35B5 nasal spray might be potential in strengthening SARS-CoV-2 prevention, especially in the high-risk population.
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http://dx.doi.org/10.1093/cid/ciac448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214129PMC
June 2022

Biochar-based microbial agent reduces U and Cd accumulation in vegetables and improves rhizosphere microecology.

J Hazard Mater 2022 08 13;436:129147. Epub 2022 May 13.

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. Electronic address:

Microbial remediation of heavy metals in soil has been widely studied. However, bioremediation efficiency is limited in practical applications because of nutritional deficiency, low efficiency, and competition with indigenous microorganisms. Herein, we prepared a biochar-based microbial agent (BMA) by immobilizing the microbial agent (MA, containing Bacillus subtilis, Bacillus cereus, and Citrobacter sp.) on biochar for the remediation of U and Cd in soil. The results showed that BMA increased soil organic matter, cation exchange capacity, and fluorescein diacetate hydrolysis activity and dehydrogenase activity by 58.7%, 38.2%, 42.9%, and 51.1%. The availability of U and Cd were significantly decreased by 67.4% and 54.2% in BMA amended soil, thereby reducing their accumulation in vegetables. BMA greatly promoted vegetable growth. Additionally, BMA significantly altered the structure and function of rhizosphere soil microbial communities. Coincidently, more abundant ecologically beneficial bacteria like Nitrospira, Nitrosomonas, Lysobacter, and Bacillus were observed, whereas plant pathogenic fungi like Fusarium and Alternaria reduced in BMA amended soil. The network analysis revealed that BMA amendment increased the tightness and complexity of microbial communities. Importantly, the compatibility of niches and microbial species within co-occurrence network was enhanced after BMA addition. These findings provide a promising strategy for suppressing heavy metal accumulation in vegetables and promoting their growth.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129147DOI Listing
August 2022

Comprehensive Characterization of the Genomic Landscape in Chinese Pulmonary Neuroendocrine Tumors Reveals Prognostic and Therapeutic Markers (CSWOG-1901).

Oncologist 2022 03;27(2):e116-e125

The Second Department of Thoracic Oncology, Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, People's Republic of China.

Background: Pulmonary neuroendocrine tumors (pNETs) include typical carcinoid (TC), atypical carcinoid (AC), large cell neuroendocrine carcinoma (LCNEC), and small cell lung carcinoma (SCLC). The optimal treatment strategy for each subtype remains elusive, partly due to the lack of comprehensive understanding of their molecular features. We aimed to explore differential genomic signatures in pNET subtypes and identify potential prognostic and therapeutic biomarkers.

Methods: We investigated genomic profiles of 57 LCNECs, 49 SCLCs, 18 TCs, and 24 ACs by sequencing tumor tissues with a 520-gene panel and explored the associations between genomic features and prognosis.

Results: Both LCNEC and SCLC displayed higher mutation rates for TP53, PRKDC, SPTA1, NOTCH1, NOTCH2, and PTPRD than TC and AC. Small cell lung carcinoma harbored more frequent co-alterations in TP53-RB1, alterations in PIK3CA and SOX2, and mutations in HIF-1, VEGF and Notch pathways. Large cell neuroendocrine carcinoma (12.7 mutations/Mb) and SCLC (11.9 mutations/Mb) showed higher tumor mutational burdens than TC (2.4 mutations/Mb) and AC (7.1 mutations/Mb). 26.3% of LCNECs and 20.8% of ACs harbored alterations in classical non-small cell lung cancer driver genes. The presence of alterations in the homologous recombination pathway predicted longer progression-free survival in advanced LCNEC patients with systemic therapy (P = .005) and longer overall survival (OS) in SCLC patients with resection (P = .011). The presence of alterations in VEGF (P = .048) and estrogen (P = .018) signaling pathways both correlated with better OS in patients with resected SCLC.

Conclusion: We performed a comprehensive genomic investigation on 4 pNET subtypes in the Chinese population. Our data revealed distinctive genomic signatures in subtypes and provided new insights into the prognostic and therapeutic stratification of pNETs.
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http://dx.doi.org/10.1093/oncolo/oyab044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8895731PMC
March 2022

Atomic investigations on the tension-compression asymmetry of AlFeNiCrCu ( = 0.5, 1.0, 1.5, 2.0) high-entropy alloy nanowires.

Nanotechnology 2022 Jul 25;33(41). Epub 2022 Jul 25.

Key Laboratory of CNC Equipment Reliability Ministry of Education, Jilin University, 5988 Renmin Street, Changchun 130025, People's Republic of China.

The tension and compression of high-entropy alloy (HEA) nanowires (NWs) are remarkably asymmetric, but the micro mechanism is still unclear. In this research, the tension-compression asymmetry of AlFeNiCrCu HEA NWs ( = 0.5, 1.0, 1.5, 2.0) was quantitatively characterized via molecular dynamics simulations, focusing on the influences of the NW diameter, the Al content, the crystalline orientation, and the temperature, which are significant for applying HEAs in nanotechnology. The increased NW diameter improves the energy required for stacking faults nucleating, thus strengthening AlFeNiCrCu HEA NWs. A few twins during stretching weaken the strengthening effects, thereby decreasing the tension-compression asymmetry. The increased Al content raises the tension-compression asymmetry by promoting the face-centered cubic to body-centered cubic phase transition during stretching. The tension along the [001] crystalline orientation is stronger than the compression, while the [110] and [111] crystalline orientations are entirely the opposite, and the tension-compression asymmetry along the [111] crystalline orientation is the minimum. The diversities in the tension-compression asymmetry depend on the deformation mechanism. Compressing along the [001] crystalline orientation and stretching along the [110] crystalline orientation induces twinning. Deformation along the [111] crystalline orientation only leaves stacking faults in the NWs. Therefore, the tension and compression along the [111] crystalline orientation exhibit minimal asymmetry. As the temperature rises, the tension-compression asymmetry along the [001] and [111] crystalline orientations increases, while that along the [110] crystalline orientation decreases.
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http://dx.doi.org/10.1088/1361-6528/ac74ceDOI Listing
July 2022

Theoretical Study of N-H σ-Bond Activation by Nickel(0) Complex: Reaction Mechanism, Electronic Processes, and Prediction of Better Ligand.

Inorg Chem 2022 Jun 27;61(23):8715-8728. Epub 2022 May 27.

Elements Strategy Initiative for Catalysts and Batteries (ESICB), Kyoto University, 1-30 Goryo-Ohara, Nishikyo-ku, Kyoto 615-8245, Japan.

N-H σ-bond activation of alkylamine by Ni(PCy) was investigated using density functional theory (DFT) calculations. When simple alkylamine NHMe is a reactant, both concerted oxidative addition in Ni(PCy)(NHMe) and ligand-to-ligand H transfer reaction in Ni(PCy)(CH)(NHMe) are endergonic and need a high activation energy. When NH(Me)(Bs) (Bs = SOPh, a model of tosyl group used in experiments) is a reactant, both reactions are exergonic and occur easily with a much smaller activation energy. The much larger reactivity of NH(Me)(Bs) than that of NHMe results from the stronger Ni-N(Me)(Bs) bond than the Ni-NMe bond and the presence of the Ni-O bonding interaction between the Bs group and the Ni atom in the product. -Heterocyclic carbene, 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene (IPr), is computationally predicted to be better than PCy because the Ni-NMe and Ni-N(Me)(Bs) bonds in the IPr complex are stronger, respectively, than those of the PCy complex. The introduction of the electron-withdrawing Bs group to the N atom of amine and the use of IPr as a ligand are recommended for the N-H σ-bond activation. The C-H σ-bond activations of benzene via the oxidative addition and the ligand-to-ligand H transfer reaction were also investigated here for comparison with the N-H σ-bond activation. The differences between the C-H σ-bond activation of benzene and the N-H σ-bond activation of these amines are discussed in terms of the N-H, C-H, Ni-Ph, and Ni-NMe, and Ni-N(Me)(Bs) bond energies and back-donation to benzene from the Ni atom.
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http://dx.doi.org/10.1021/acs.inorgchem.2c00612DOI Listing
June 2022

Association Between Glycemic Gap and Mortality in Critically Ill Patients with Diabetes.

J Intensive Care Med 2022 May 25:8850666221101856. Epub 2022 May 25.

Department of Critical Care Medicine, 71043Fu Xing Hospital, Capital Medical University, 20A Fuxingmenwai Street, Xicheng District, Beijing 100038, China.

Objectives: Dysglycemia is associated with poor outcomes in critically ill patients,which is uncertain in patients with diabetes regarding to the situation of glucose control before hospitalization. This study was aimed to investigate the effect of the difference between the level of blood glucose during ICU stay and before admission to ICU upon the outcomes of critically ill patients with diabetes.

Method: Patients with diabetes expected to stay for more than 24hs were enrolled, HbA1c was converted to A1C-derived average glucose (ADAG) by the equation: ADAG  =  [ (HbA1c * 28.7) - 46.7 ] * 18, blood glucose were measured four times a day during the first 7 days after admission, the mean glucose level(MGL) and SOFA (within 3, 5, and 7days) were calculated for each person, GAP and GAP was calculated as admission blood glucose and MGL minus ADAG, the incidence of moderate hypoglycemia(MH), severe hypoglycemia (SH), total dosage of glucocorticoids and average daily dosage of insulin, duration of renal replacement therapy(RRT), ventilator-free hours, and non-ICU days were also collected. Patients were divided into survival group and nonsurvival group according to survival or not at 28-day, the relationship between GAP and mortality were analyzed.

Results: 431 patients were divided into survival group and nonsurvival group. The two groups had a comparable level of HbA1c, the nonsurvivors had greater APACHE II, SOFA, GAP, GAP, GAP, GAP and higher MH and SH incidences. Less duration of ventilator-free, non-ICU stay and longer duration of RRT were recorded in the nonsurvival group. GAP had the greatest predictive power with an AUC of 0.807(95%CI: 0.762-0.851), the cut-off value was 3.6 mmol/L (sensitivity 77.7% and specificity 76.6%). The AUC was increased to 0.852(95%CI: 0.814-0.889) incorporated with SOFA (NRI  =  11.34%).

Conclusion: Glycemic GAP between the MGL within 5 days and ADAG was independently associated with 28-day mortality of critically ill patients with diabetes. The predictive power was optimized with addition of SOFA.
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http://dx.doi.org/10.1177/08850666221101856DOI Listing
May 2022

A Novel Reconstruction Method for Temperature Distribution Measurement Based on Ultrasonic Tomography.

IEEE Trans Ultrason Ferroelectr Freq Control 2022 Jul 30;69(7):2352-2370. Epub 2022 Jun 30.

The precise temperature distribution measurement is crucial in many industrial fields, where ultrasonic tomography (UT) has broad application prospects and significance. In order to improve the resolution of reconstructed temperature distribution images and maintain high accuracy, a novel two-step reconstruction method is proposed in this article. First, the problem of solving the temperature distribution is converted to an optimization problem and then solved by an improved version of the equilibrium optimizer (IEO), in which a new nonlinear time strategy and novel population update rules are deployed. Then, based on the low-resolution and high-precision images reconstructed by IEO, Gaussian process regression (GPR) is adopted to enhance image resolution and keep the reconstruction errors low. After that, the number of divided grids and the parameters of IEO are also further studied to improve the reconstruction quality. The results of numerical simulations and experiments indicate that high-resolution images with low reconstruction errors can be reconstructed effectively by the proposed IEO-GPR method, and it also shows excellent robust performance. For a complex three-peak temperature distribution, a competitive accuracy with 3.10% and 2.37% error at root-mean-square error and average relative error is achieved, respectively. In practical experiment, the root-mean-square error of IEO-GPR is 0.72%, which is at least 0.89% lower than that of conventional algorithms.
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http://dx.doi.org/10.1109/TUFFC.2022.3177469DOI Listing
July 2022

Shifting Rice Cropping Systems Mitigates Ecological Footprints and Enhances Grain Yield in Central China.

Front Plant Sci 2022 4;13:895402. Epub 2022 May 4.

Hubei Collaborative Innovation Center for Grain Industry, College of Agriculture, Yangtze University, Jingzhou, China.

Intensive cereal production has brought about increasingly serious environmental threats, including global warming, environmental acidification, and water shortage. As an important grain producer in the world, the rice cultivation system in central China has undergone excessive changes in the past few decades. However, few articles focused on the environmental impacts of these shifts from the perspective of ecological footprints. In this study, a 2-year field trial was carried out in Hubei province, China, to gain insight into carbon footprint (CF), nitrogen footprint (NF), and water footprint (WF) performance. The three treatments were, namely, double-rice system (DR), ratoon rice system (RR), and rice-wheat system (RW). Results demonstrated that RR significantly increased the grain yield by 10.22-15.09% compared with DR, while there was no significant difference in the grain yield between RW and DR in 2018-2019. All of the calculation results by three footprint approaches followed the order: RR < RW < DR; meanwhile, RR was always significantly lower than DR. Methane and NH field emissions were the hotspots of CF and NF, respectively. Blue WF accounts for 40.90-42.71% of DR, which was significantly higher than that of RR and RW, primarily because DR needs a lot of irrigation water in both seasons. The gray WF of RW was higher than those of DR and RR, mainly due to the higher application rate of N fertilizer. In conclusion, RR possesses the characteristics of low agricultural inputs and high grain yield and can reduce CF, NF, and WF, considering the future conditions of rural societal developments and rapid demographic changes; we highlighted that the RR could be a cleaner and sustainable approach to grain production.
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http://dx.doi.org/10.3389/fpls.2022.895402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115467PMC
May 2022

Tumor-induced erythroid precursor-differentiated myeloid cells mediate immunosuppression and curtail anti-PD-1/PD-L1 treatment efficacy.

Cancer Cell 2022 06 19;40(6):674-693.e7. Epub 2022 May 19.

Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Chongqing Key Laboratory of Immunotherapy, Chongqing, China. Electronic address:

Despite the unprecedented success of immune checkpoint inhibitors (ICIs) as anti-cancer therapy, it remains a prevailing clinical need to identify additional mechanisms underlying ICI therapeutic efficacy and potential drug resistance. Here, using lineage tracking in cancer patients and tumor-bearing mice, we demonstrate that erythroid progenitor cells lose their developmental potential and switch to the myeloid lineage. Single-cell transcriptome analyses reveal that, notwithstanding quantitative differences in erythroid gene expression, erythroid differentiated myeloid cells (EDMCs) are transcriptionally indistinguishable from their myeloid-originated counterparts. EDMCs possess multifaceted machinery to curtail T cell-mediated anti-tumor responses. Consequently, EDMC content within tumor tissues is negatively associated with T cell inflammation for the majority of solid cancers; moreover, EDMC enrichment, in accordance with anemia manifestation, is predictive of poor prognosis in various cohorts of patients undergoing ICI therapy. Together, our findings reveal a feedforward mechanism by which tumors exploit anemia-triggered erythropoiesis for myeloid transdifferentiation and immunosuppression.
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http://dx.doi.org/10.1016/j.ccell.2022.04.018DOI Listing
June 2022

Comment on: "Metabolic syndrome in adolescents and antioxidant nutrient intake: a cross-sectional study".

Authors:
Bo Zhu Lianping He

Rev Assoc Med Bras (1992) 2022 05;68(5):558

Taizhou University, School of Medicine - Taizhou, China.

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http://dx.doi.org/10.1590/1806-9282.20220099DOI Listing
May 2022

Adhesion behaviors of water droplets on bioinspired superhydrophobic surfaces.

Bioinspir Biomim 2022 06 23;17(4). Epub 2022 Jun 23.

School of Power and Mechanical Engineering, The Institute of Technological Science, Wuhan University, South Donghu Road 8, 430072, Wuhan, Hubei Province, People's Republic of China.

The adhesion behaviors of droplets on surfaces are attracting increasing attention due to their various applications. Many bioinspired superhydrophobic surfaces with different adhesion states have been constructed in order to mimic the functions of natural surfaces such as a lotus leaf, a rose petal, butterfly wings, etc. In this review, we first present a brief introduction to the fundamental theories of the adhesion behaviors of droplets on various surfaces, including low adhesion, high adhesion and anisotropic adhesion states. Then, different techniques to characterize droplet adhesion on these surfaces, including the rotating disk technique, the atomic force microscope cantilever technique, and capillary sensor-based techniques, are described. Wetting behaviors, and the switching between different adhesion states on bioinspired surfaces, are also summarized and discussed. Subsequently, the diverse applications of bioinspired surfaces, including water collection, liquid transport, drag reduction, and oil/water separation, are discussed. Finally, the challenges of using liquid adhesion behaviors on various surfaces, and future applications of these surfaces, are discussed.
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http://dx.doi.org/10.1088/1748-3190/ac6fa5DOI Listing
June 2022

Micro-synteny conservation analysis revealed the evolutionary history of bacterial biphenyl degradation pathway.

Environ Microbiol Rep 2022 Aug 12;14(4):494-505. Epub 2022 May 12.

Department of Environmental Science, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, 87300, Pakistan.

Phenolic compounds have been enlisted by the United States Environmental Protection Agency (USEPA) and the European Union (EU) as pollutants of priority concern. The biphenyl degradation pathway plays an essential role in prokaryote polychlorinated biphenyls degradation. Our understanding of prokaryotic pathways and their evolution has dramatically increased in recent years with the advancements in prokaryotic genome sequencing and analysis tools. In this work, we applied bioinformatics tools to study the evolution of the biphenyl degradation pathway focusing on the phylogeny and initiation of four representative species (Burkholderia xenovorans LB400, Polaromonas naphthalenivorans CJ2, Pseudomonas putida F1 and Rhodococcus jostii RHA1). These species contained partial or full concatenated genes from bph gene cluster (i.e. bphRbphA1A2A3A4BCKHJID). The aim was to establish this pathway's origin and development mode in the prokaryotic world. Genomic screening revealed that many bacterial species possess genes for the biphenyl degradation pathway. However, the micro-synteny conservation analysis indicated that massive gene recruitment events might have occurred during the evolution of the biphenyl degradation pathway. Combining with the phylogenetic positions, this work points to the evolutionary process of acquiring the biphenyl degradation pathway by different fragments through horizontal gene transfer in these bacterial groups. This study reports the first-ever evidence of the birth of this pathway in the represented species.
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http://dx.doi.org/10.1111/1758-2229.13081DOI Listing
August 2022

Rubus chingii Hu. unripe fruits extract ameliorates carbon tetrachloride-induced liver fibrosis and improves the associated gut microbiota imbalance.

Chin Med 2022 May 12;17(1):56. Epub 2022 May 12.

Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Shanghai, 201203, China.

Background: The unripe fruits of Rubus chingii Hu. ("Fu-peng-zi" in Chinese) is a well-known herbal tonic in traditional Chinese medicine (TCM) for tonifying liver and kidney. However, little is known regarding its therapeutic efficacy against liver fibrosis and the underlying mechanism.

Methods: The current research aims to explore the potential of Rubus chingii Hu. unripe fruits extract (RF) in the treatment of liver fibrosis and explore the underlying mechanism. RF was administered (450 and 900 mg·kg of body weight per day) orally to male C57BL/6 mice with CCl-induced liver fibrosis for 3 weeks. The histopathological changes and fibrosis stage in liver tissue were assessed using hematoxylin and eosin (H&E) and Sirius red staining. The distribution of α-SMA and Col1A1 in the liver was analyzed to determine the hepatic stellate cells (HSCs) activation using immunohistochemistry and immunofluorescent analysis. Various biochemical markers in serum (ALT, AST) and liver (Hyp, IL1-β, IL6, TNF-α and MCP-1) were observed to assess the liver's injury, fibrosis, and inflammation. In liver tissue, fibrosis-associated proteins including α-SMA, TGF-β1, Smad2/3, p-Smad2/3, and Smad4 were detected through a Western blot assay. Pyrosequencing-based analysis of bacterial 16 S ribosomal RNA from variable regions V3-V4 of fecal samples characterized the gut microbiota. Spearman's rank correlation analysis was performed for the association between altered bacterial genera by RF and pharmacodynamics parameters.

Results: Three weeks of RF treatment can significantly lower liver inflammatory levels, pathological abnormalities, and collagen fibrous deposition in mice with CCl-induced liver fibrosis. The expressions of α-SMA and Col1A1 were lowered by RF, while the expression levels of TGF-β/Smads signaling pathway-related proteins, including TGF-β1, p-Smad2/3, and Smad4, were dramatically decreased by RF. The RF treatment significantly increased or reduced 18 different bacterial species, restoring the CCl-induced gut microbiota imbalance to the normal group's levels. According to correlation analysis, the bacterial genera Bifidobacterium and Turicibacter were the most significant in restoring CCl-induced liver fibrosis.

Conclusions: RF can reduce liver damage and delay the onset of liver fibrosis through modulating TGF-β/Smads signaling pathway. Furthermore, RF's anti-liver fibrosis effect was related to balancing the gut microbial community, partly attained by increasing Bifidobacterium and Turicibacter in liver fibrosis.
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http://dx.doi.org/10.1186/s13020-022-00607-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097331PMC
May 2022

Theoretical insight into decatungstate photocatalyzed alkylation of -tosylimine hydrogen atom transfer and proton-coupled electron transfer.

Dalton Trans 2022 May 24;51(20):7928-7935. Epub 2022 May 24.

Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China.

Decatungstate as a photocatalyst can activate various C(sp)-H bonds to successfully construct the C(sp)-C(sp) bond with -tosylimines. Herein density functional theory (DFT) calculations reveal a unique radical mechanism triggered by the reductive quenching cycle of decatungstate. First of all, photoexcited *[WO] activates the C(sp)-H bond of ether through the hydrogen atom transfer (HAT) mechanism to generate [HWO] and a C-centered radical species. Next, the C-centered radical will selectively attack the imine carbon of -tosylimine to provide the N-centered radical species containing the C(sp)-C(sp) bond. Finally, the C(sp)-C(sp) coupling product can be afforded by the stepwise proton-coupled electron transfer (PCET) process between [HWO] and the N-centered radical. Importantly, the bridging oxygen in the lateral position of [WO] is the most active. Intrinsic bond orbital (IBO) analysis confirms that *[WO] activates C(sp)-H through HAT instead of PCET. Furthermore, the origin of the regio-selectivity has been explored in depth. We hope that the reductive quenching cycle mechanism ([WO]-*[WO]-[HWO]-[WO]) can provide a clear understanding of the alkylation of -tosylimine.
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http://dx.doi.org/10.1039/d2dt00927gDOI Listing
May 2022

SeNic: An Open Source Dataset for sEMG-Based Gesture Recognition in Non-Ideal Conditions.

IEEE Trans Neural Syst Rehabil Eng 2022 17;30:1252-1260. Epub 2022 May 17.

In order to reduce the gap between the laboratory environment and actual use in daily life of human-machine interaction based on surface electromyogram (sEMG) intent recognition, this paper presents a benchmark dataset of sEMG in non-ideal conditions (SeNic). The dataset mainly consists of 8-channel sEMG signals, and electrode shifts from an 3D-printed annular ruler. A total of 36 subjects participate in our data acquisition experiments of 7 gestures in non-ideal conditions, where non-ideal factors of 1) electrode shifts, 2) individual difference, 3) muscle fatigue, 4) inter-day difference, and 5) arm postures are elaborately involved. Signals of sEMG are validated first in temporal and frequency domains. Results of recognizing gestures in ideal conditions indicate the high quality of the dataset. Adverse impacts in non-ideal conditions are further revealed in the amplitudes of these data and recognition accuracies. To be concluded, SeNic is a benchmark dataset that introduces several non-ideal factors which often degrade the robustness of sEMG-based systems. It could be used as a freely available dataset and a common platform for researchers in the sEMG-based recognition community. The benchmark dataset SeNic are available online via the website (https://github.com/bozhubo/SeNic and https://gitee.com/bozhubo/SeNic).
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http://dx.doi.org/10.1109/TNSRE.2022.3173708DOI Listing
May 2022

A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex.

Plant Cell 2022 07;34(8):2969-2988

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.

Polycomb repressive complex 2 (PRC2), which mediates the deposition of H3K27me3 histone marks, is important for developmental decisions in animals and plants. In the shoot apical meristem (SAM), Three Amino acid Loop Extension family KNOTTED-LIKE HOMEOBOX /BEL-like (KNOX/BELL) transcription factors are key regulators of meristem cell pluripotency and differentiation. Here, we identified a PRC2-associated coiled-coil protein (PACP) that interacts with KNOX/BELL transcription factors in rice (Oryza sativa) shoot apex cells. A loss-of-function mutation of PACP resulted in differential gene expression similar to that observed in PRC2 gene knockdown plants, reduced H3K27me3 levels, and reduced genome-wide binding of the PRC2 core component EMF2b. The genomic binding of PACP displayed a similar distribution pattern to EMF2b, and genomic regions with high PACP- and EMF2b-binding signals were marked by high levels of H3K27me3. We show that PACP is required for the repression of cell differentiation-promoting genes targeted by a rice KNOX1 protein in the SAM. PACP is involved in the recruitment or stabilization of PRC2 to genes targeted by KNOX/BELL transcription factors to maintain H3K27me3 and gene repression in dividing cells of the shoot apex. Our results provide insight into PRC2-mediated maintenance of H3K27me3 and the mechanism by which KNOX/BELL proteins regulate SAM development.
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http://dx.doi.org/10.1093/plcell/koac133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338815PMC
July 2022
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