Publications by authors named "Bo Zhou"

1,275 Publications

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[Effect of free deep inferior epigastric artery perforator flap transplantation in breast shaping of two-staged breast reconstruction with vertical scar].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Oct;35(10):1323-1327

Ultrasound Diagnosis Center, Hunan Province Cancer Hospital, Changsha Hunan, 410008, P.R.China.

Objective: To summarize the difficulties and key points of free deep inferior epigastric artery perforator flap (DIEP) transplantation in breast shaping of two-staged breast reconstruction with vertical scar.

Methods: The clinical data of 32 postoperative breast cancer patients after mastectomy who underwent free DIEP transplantation for breast reconstruction between October 2015 and October 2019, whose original surgical incisions were all vertical and longitudinal, were retrospectively analysed. All the patients were female, aged from 31 to 42 years, with an average of 34.6 years. The disease duration of breast cancer ranged from 9 to 48 months (mean, 22.8 months). Free DIEP pedicled with contralateral vessels were used in all cases, and the recipient vessels were intrathoracic vessels. Among them, 17 flaps were placed longitudinally, 15 flaps were placed obliquely; Z-shaped flaps were used in 18 cases to adjust the chest wall skin contracture, and contralateral breast reduction and mastopexy were accomplished at the same time in 23 cases.

Results: All DIEP survived completely, and all donor and recipient sites healed by first intention. Internal thoracic lymph node metastasis was found in 1 case and treated with radiotherapy. All 32 cases were followed up 9-48 months, with an average of 19.4 months. The appearance and texture of all flaps were satisfactory, and only linear scar was left in donor site. Eleven patients underwent further autologous fat transplantation and nipple reconstruction. All patients had no effect on abdominal wall activity, and no local recurrence and metastasis was found.

Conclusion: It is difficult to use free DIEP for two-staged breast reconstruction in patients received mastectomy with vertical longitudinal scar left. Combined with different breast shaping techniques, the outcomes can significantly improve.
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http://dx.doi.org/10.7507/1002-1892.202105016DOI Listing
October 2021

100 kHz CHO imaging realized by lower speed planar laser-induced fluorescence and deep learning.

Opt Express 2021 Sep;29(19):30857-30877

This paper reports an approach to interpolate planar laser-induced fluorescence (PLIF) images of CHO between consecutive experimental data by means of computational imaging realized with convolutional neural network (CNN). Such a deep learning based method can achieve higher temporal resolution for 2D visualization of intermediate species in combustion based on high-speed experimental images. The capability of the model was tested for generating 100 kHz PLIF images by interpolating single and multiple PLIF frames into the sequences of experimental images of lower frequencies (50, 33, 25 and 20 kHz). Results show that the prediction indices, including intersection over union (IoU), peak signal to noise ratio (PSNR), structural similarity index (SSIM), and time averaged correlation coefficient at various axial positions could achieve acceptable accuracy. This work sheds light on the utilization of CNN-based models to achieve optical flow computation and image sequence interpolation, also providing an efficient off-line model as an alternative pathway to overcome the experimental challenges of the state-of-the-art ultra-high speed PLIF techniques, e.g., to further increase repetition rate and save data transfer time.
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http://dx.doi.org/10.1364/OE.433785DOI Listing
September 2021

The Sichuan Mental Health Survey: Methodology.

Front Psychiatry 2021 17;12:645355. Epub 2021 Sep 17.

Key Laboratory of Psychosomatic Medicine, Chinese Academy of Medical Sciences, Chengdu, China.

The Sichuan Mental Health Survey (SMHS) is a provincially representative survey with a coherent methodology to obtain the prevalence of multiple mental disorders and data of services used and to analyze the psychological and social risk factors or correlates in Sichuan, China. Mental disorders include anxiety disorders, mood disorders, schizophrenia, and other psychotic disorders, drug use and alcohol use disorders, impulse control disorder, and eating disorders. A cross-sectional design is employed to sample adults from 200 communities/villages in all 21 prefectural-level municipalities of Sichuan Province in a five-stage provincially representative disproportionate stratified sampling design. The participants need to be interviewed face to face by trained interviewers from local primary healthcare institutions and by psychiatrists. The quality control staff implement data quality control by checking records and statistics in the interview system, and then re-interviewing checks are done by the psychiatrists. Data is weighted to adjust the sample distribution to match the whole population. The outcomes of the SMHS would not only demonstrate the serious challenges posed by the high burdens of mental disorders but also offer baseline data for policymakers and healthcare professionals to study and resolve the factors that influence mental health in Sichuan, China.
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http://dx.doi.org/10.3389/fpsyt.2021.645355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484526PMC
September 2021

Impact of Age, Sex, and Renal Function on the Efficacy and Safety of Direct Oral Anticoagulants vs. Vitamin K Antagonists for the Treatment of Acute Venous Thromboembolism: A Meta-Analysis of 22,040 Patients.

Front Cardiovasc Med 2021 8;8:700740. Epub 2021 Sep 8.

Cardiovascular Department, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

In this study, we conducted a meta-analysis to assess the impact of age, sex, and renal function on the efficacy and safety of direct oral anticoagulants (DOACs) vs. vitamin K antagonists (VKAs) for the treatment of acute venous thromboembolism (VTE). Electronic databases (accessed till June 2021) were systematically searched to investigate randomized clinical trials evaluating apixaban, dabigatran, edoxaban, and rivaroxaban vs. VKAs for the treatment of acute VTE. Results were presented as odds ratio (OR) and 95% CIs. Direct oral anticoagulants were associated with a borderline higher efficacy in women (OR: 0.79, 95% CI: 0.62-1.02), a significantly higher efficacy in patients with age more than 75 years (OR: 0.51, 95% CI: 0.32-0.80), and creatinine clearance <50 ml/min (OR: 0.57, 95% CI: 0.32-0.99). The primary safety endpoint of major or clinically relevant non-major bleeding was significantly reduced in DOACs as compared to VKAs in both patients with age <75 years (OR: 0.79, 95% CI: 0.70-0.89) and patients with age more than 75 years (OR: 0.75, 95% CI: 0.59-0.96). DOACs also show an advantage in terms of major or clinically relevant non-major bleeding in men (OR: 0.72, 95% CI: 0.60-0.86) and patients with creatinine clearance of more than 50 ml/min (OR: 0.75, 95% CI: 0.67-0.84). Direct oral anticoagulants have exhibited clinical preference among patients with acute VTE with decreased thrombosis and bleeding events, especially in patients with age more than 75 years and creatinine clearance <50 ml/min.
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http://dx.doi.org/10.3389/fcvm.2021.700740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455915PMC
September 2021

Coupling Glucose-Assisted Cu(I)/Cu(II) Redox with Electrochemical Hydrogen Production.

Adv Mater 2021 Sep 24:e2104791. Epub 2021 Sep 24.

State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, P. R. China.

Water electrolysis is a sustainable technology for hydrogen production since this process can utilize the intermittent electricity generated by renewable energy such as solar, wind, and hydro. However, the large-scale application of this process is restricted by the high electricity consumption due to the large potential gap (>1.23 V) between the anodic oxygen evolution reaction and the cathodic hydrogen evolution reaction (HER). Herein, a novel and efficient hydrogen production system is developed for coupling glucose-assisted Cu(I)/Cu(II) redox with HER. The onset potential of the electrooxidation of Cu(I) to Cu(II) is as low as 0.7 V (vs reversible hydrogen electrode). In situ Raman spectroscopy, ex situ X-ray photoelectron spectroscopy, and density functional theory calculation demonstrates that glucose in the electrolyte can reduce the Cu(II) into Cu(I) instantaneously via a thermocatalysis process, thus completing the cycle of Cu(I)/Cu(II) redox. The assembled electrolyzer only requires a voltage input of 0.92 V to achieve a current density of 100 mA cm . Consequently, the electricity consumption for per cubic H produced in the system is 2.2 kWh, only half of the value for conventional water electrolysis (4.5 kWh). This work provides a promising strategy for the low-cost, efficient production of high-purity H .
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http://dx.doi.org/10.1002/adma.202104791DOI Listing
September 2021

Core-shell [email protected] upconversion nanoparticles qualify as a NIR speckle wavemeter for a visible CCD.

Nanoscale 2021 Oct 8;13(38):16207-16215. Epub 2021 Oct 8.

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, and Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, South China University of Technology, Guangzhou 510641, China.

Speckle patterns have been widely employed as a method for precisely determining the wavelength of monochromatic light. In order to achieve higher wavelength precision, a variety of optical diffusing waveguides have been investigated with a focus on their wavelength sensitivity. However, it has been a challenge to find a balance among the cost, compactness, precision, and stability of the waveguide. In this work, we designed a compact cylindrical random scattering waveguide (CRSW) as the light diffuser by mixing TiO particles and ultra-violate adhesive. In the CRSW, speckle patterns are generated by input light scattering off TiO particles multiple times. Additionally, a thin layer of upconversion nanoparticles (UCNPs) was sprayed on the end face of CRSW to allow near-infrared (NIR) light to be converted to visible light, breaking the imaging limitations of visible cameras in the NIR range. We, then, further designed a convolution neural network (CNN) to recognize the wavelength of the speckle patterns with excellent robustness and ability to transfer learning. This resulted in the achievement of a high wavelength precision of 20 kHz (∼0.16 fm) at around 1550 nm with a temperature resistance of ±2 °C. Our results demonstrate a low-cost, compact, and simple NIR wavemeter, which is capable of ultra-high wavelength precision and good temperature stability. It has significant value for applications in high-speed and high-precision laser wavelength measurements.
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http://dx.doi.org/10.1039/d1nr03932fDOI Listing
October 2021

Creation of an Anti-Inflammatory, Leptin-Dependent Anti-Obesity Celastrol Mimic with Better Druggability.

Front Pharmacol 2021 30;12:705252. Epub 2021 Aug 30.

Laboratory of Natural Medicine and Molecular Engineering, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Obesity is characterized by an excessive body mass, but is also closely associated with metabolic syndrome. And, so far, only limited pharmacological treatments are available for obesity management. Celastrol, a pentacyclic triterpenoid from a traditional Chinese medicine ( Hook.f.), has shown remarkable potency against obesity, inflammation and cancer, but its high toxicity, low natural abundance and tedious chemical synthesis hindered its translation into clinics. In the present work, a triterpenoid library was screened for compounds with both high natural abundance and structural similarity to celastrol; from this library, glycyrrhetinic acid (GA), a compound present in extremely high yields in Fisch. ex DC., was selected as a possible scaffold for a celastrol mimic active against obesity. A simple chemical modification of GA resulted in GA-02, a derivative that suppressed 68% of food intake in diet-induced obesity mice and led to 26.4% weight loss in 2 weeks. GA-02 plays a role in obesity treatment by re-activating leptin signaling and reducing systemic and, more importantly, hypothalamic inflammation. GA-02 was readily bioavailable with unnoticeable and toxicities. The strategy of scaffold search and modification on the basis of bio-content and structural similarity has proved to be a green, economic, efficient and practical way of widening the medicinal applications of "imperfect" bioactive natural compounds.
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http://dx.doi.org/10.3389/fphar.2021.705252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435713PMC
August 2021

[Application of modified designed bilobed latissimus dorsi myocutaneous flap in chest wall reconstruction of locally advanced breast cancer patients].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Sep;35(9):1172-1176

Department of Oncology Plastic Surgery, Hunan Province Cancer Hospital, Changsha Hunan, 410008, P.R.China.

Objective: To explore the effectiveness of the modified designed bilobed latissimus dorsi myocutaneous flap in chest wall reconstruction of locally advanced breast cancer (LABC) patients.

Methods: Between January 2016 and June 2019, 64 unilateral LABC patients were admitted. All patients were female with an average age of 41.3 years (range, 34-50 years). The disease duration ranged from 6 to 32 months (mean, 12.3 months). The diameter of primary tumor ranged from 4.8 to 14.2 cm (mean, 8.59 cm). The size of chest wall defect ranged from 16 cm×15 cm to 20 cm×20 cm after modified radical mastectomy/radical mastectomy. All defects were reconstructed with the modified designed bilobed latissimus dorsi myocutaneous flaps, including 34 cases with antegrade method and 30 cases with retrograde method. The size of skin paddle ranged from 13 cm×5 cm to 17 cm×6 cm. All the donor sites were closed directly.

Results: In antegrade group, 2 flaps (5.8%, 2/34) showed partial necrosis; in retrograde group, 6 flaps (20%, 6/30) showed partial necrosis, 5 donor sites (16.7%, 5/30) showed partial necrosis; and all of them healed after dressing treatment. The other flaps survived successfully and incisions in donor sites healed by first intention. There was no significant difference in the incidence of partial necrosis between antegrade and retrograde groups ( =2.904, =0.091). The difference in delayed healing rate of donor site between the two groups was significant ( =0.013). The patients were followed up 15-30 months, with an average of 23.1 months. The appearance and texture of the flaps were satisfactory, and only linear scar left in the donor site. No local recurrence was found in all patients. Four patients died of distant metastasis, including 2 cases of liver metastasis, 1 case of brain metastasis, and 1 case of lung metastasis. The average survival time was 22.6 months (range, 20-28 months).

Conclusion: The modified designed bilobed latissimus dorsi myocutaneous flap can repair chest wall defect after LABC surgery. Antegrade design of the flap can ensure the blood supply of the flap and reduce the tension of the donor site, decrease the incidence of complications.
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http://dx.doi.org/10.7507/1002-1892.202102049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444136PMC
September 2021

Tracing the skeletal progenitor transition during postnatal bone formation.

Cell Stem Cell 2021 Sep 6. Epub 2021 Sep 6.

State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China; State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, China. Electronic address:

Multiple distinct types of skeletal progenitors have been shown to contribute to endochondral bone development and maintenance. However, the division of labor and hierarchical relationship between different progenitor populations remain undetermined. Here we developed dual-recombinase fate-mapping systems to capture the skeletal progenitor transition during postnatal bone formation. We showed that postnatal osteoblasts arose primarily from chondrocytes before adolescence and from Lepr bone marrow stromal cells (BMSCs) after adolescence. This transition occurred in the diaphysis during adolescence and progressively spread to the metaphysis. The osteoblast-forming Lepr BMSCs derived primarily from fetal Col2 cells. Conditional deletion of Runx2 from perinatal chondrocytes and adult Lepr BMSCs impaired bone lengthening and thickening, respectively. Forced running increased osteoblast formation by perinatal chondrocytes but not by adult Lepr BMSCs. Thus, the short-term developmental skeletal progenitors generated the long-term adult skeletal progenitors. They sequentially control the growth and maintenance of endochondral bones.
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http://dx.doi.org/10.1016/j.stem.2021.08.010DOI Listing
September 2021

Microbiota-derived lactate promotes hematopoiesis and erythropoiesis by inducing stem cell factor production from leptin receptor+ niche cells.

Exp Mol Med 2021 Sep 9;53(9):1319-1331. Epub 2021 Sep 9.

Mucosal Immunology Laboratory, Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Although functional interplay between intestinal microbiota and distant sites beyond the gut has been identified, the influence of microbiota-derived metabolites on hematopoietic stem cells (HSCs) remains unclear. This study investigated the role of microbiota-derived lactate in hematopoiesis using mice deficient in G-protein-coupled receptor (Gpr) 81 (Gpr81), an established lactate receptor. We detected significant depletion of total HSCs in the bone marrow (BM) of Gpr81 mice compared with heterogenic (Gpr81) mice in a steady state. Notably, the expression levels of stem cell factor (SCF), which is required for the proliferation of HSCs, decreased significantly in leptin receptor-expressing (LepR) mesenchymal stromal cells (MSCs) around the sinusoidal vessels of the BM from Gpr81 mice compared with Gpr81 mice. Hematopoietic recovery and activation of BM niche cells after irradiation or busulfan treatment also required Gpr81 signals. Oral administration of lactic acid-producing bacteria (LAB) activated SCF secretion from LepR BM MSCs and subsequently accelerated hematopoiesis and erythropoiesis. Most importantly, LAB feeding accelerated the self-renewal of HSCs in germ-free mice. These results suggest that microbiota-derived lactate stimulates SCF secretion by LepR BM MSCs and subsequently activates hematopoiesis and erythropoiesis in a Gpr81-dependent manner.
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http://dx.doi.org/10.1038/s12276-021-00667-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492757PMC
September 2021

Visualizing, quantifying, and controlling local hydrodynamic effects on biofilm accumulation in complex flow paths.

J Hazard Mater 2021 08 28;416:125937. Epub 2021 Apr 28.

College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China. Electronic address:

Complex flow paths (CFPs) are commonly applied in precision equipment to accurately supply controllable fluids with designed structures. However, the presence of biofilms in CFPs causes quite a few unwanted issues, such as bio-erosion, clogging, or even health risks. To date, visualizing and quantifying the interaction between biofilm distribution and local hydrodynamics remains difficult, and the mechanism during the process is unclear. In this paper, the remodeling simulation method (3D industrial computed tomography scanning-inverse modeling-numerical simulation) and 16S rRNA high-throughput sequencing were integrated. The results indicated that local hydrodynamic characteristics significantly affected biofilm thicknesses on CFP surfaces (relative differences of 41.3-71.2%), which inversely influenced the local turbulence intensity. The average biofilm thicknesses exhibited a significant quadratic correlation with the near-wall hydraulic shear forces (r > 0.72, p < 0.05), and the biofilm reached a maximum thickness at 0.36-0.45 Pa. On the other hand, the near-wall hydraulic shear forces not only affected microbial community characteristics of biofilms, but they also influenced the number of microorganisms involved, which determined the biofilm accumulation thereafter. The PHYLUM Firmicutes and Proteobacteria were the dominant bacteria during the process. The results obtained in this paper could provide practical conceptions for the targeted control of biofilms and put forward more efficient controlling methods in commonly applied CFP systems.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125937DOI Listing
August 2021

Enhancing upconversion of manganese through spatial control of energy migration for multi-level anti-counterfeiting.

Nanoscale 2021 Sep 10;13(33):13995-14000. Epub 2021 Aug 10.

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and Devices, South China University of Technology, Guangzhou 510641, China.

The upconversion of manganese (Mn) exhibits a green light output with a much longer lifetime than that of lanthanide ions, showing great potential in the frontier applications like information security and anti-counterfeiting. Mn can be activated by energy migration upconversion. However, there exists serious quenching interactions between Mn and the lanthanides at the core-shell interfacial area, which would markedly reduce the role of Tm as a ladder to facilitate the up-transition and subsequently limit the upconversion of Mn. Here, we propose a mechanistic strategy to enhance the upconversion luminescence of Mn by spatial control of energy migration among Gd sublattice through introducing an additional migratory NaGdF interlayer within the commonly used core-shell nanostructure. This design can not only isolate the interfacial quenching interactions between the sensitized core and luminescent shell, but also allow an efficient channel for energy transport, resulting in enhanced upconversion of Mn. Moreover, the relatively long lifetime of Mn (around 32.861 ms) provides new possibilities to utilize the temporal characteristic for the frontier application of multi-level anti-counterfeiting through combining the time-gating technology.
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http://dx.doi.org/10.1039/d1nr03836bDOI Listing
September 2021

Isolation, Purification, Characterization, and Immunomodulatory Activity Analysis of α-Glucans from .

ACS Omega 2021 Aug 12;6(33):21384-21394. Epub 2021 Aug 12.

College of Food and Biological Engineering, Jimei University, Xiamen 361021, P. R. China.

Crude polysaccharides from (SP) were isolated by maceration with a hot alkali solution and further fractionated by DEAE-52 cellulose and Sephadex G-100 chromatography into two purified fractions PSP-1 and PSP-2. The monosaccharide composition analysis indicated that SP was mainly composed of rhamnose and glucose, while PSP-1 and PSP-2 were composed only of glucose. The composition analysis of PSP-1 and PSP-2 by HPLC, FT-IR, and NMR showed that PSP-1 and PSP-2 were branching dextran, and their structures were (1 → 4)-linked-α-D-Glcp as the main chain, and C-6 replaced the single α-D-Glcp as the linear structure of the branch chain. The glucans (SP/PSP-1/PSP-2) can significantly improve the phagocytic ability of macrophages, enhance iNOS activity, promote NO production, and increase IL-6 mRNA expression, so they may possess certain immunomodulatory activity.
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http://dx.doi.org/10.1021/acsomega.1c02175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387993PMC
August 2021

Effect of Salt Substitution on Cardiovascular Events and Death.

N Engl J Med 2021 09 29;385(12):1067-1077. Epub 2021 Aug 29.

From the George Institute for Global Health (B.N., M.T., L.H., Y.L., X.Y., J.Y., K.-C.L., G.L.D.T., S. Stepien, S. Shan) and the Centre for Big Data Research in Health (S.-A.P.), University of New South Wales, and George Clinical (N.L.) - all in Sydney; the School of Public Health (B.N., K.-C.L., P.E.), the U.K. Dementia Research Institute (P.E.), the British Heart Foundation Centre for Research Excellence (P.E.), and the NIHR Imperial Biomedical Research Centre (P.E.), Imperial College London, Health Data Research (P.E.), the NIHR Health Protection Research Unit in Chemical and Radiation Threats and Hazards (P.E.), and the Medical Research Council Centre for Environment and Health (P.E.) - all in London; Peking University Clinical Research Center, Peking University (Y.W.), the George Institute for Global Health at Peking University Health Science Center (Y.W., M.T., Z.H., X.Z., L.L.Y.), and the Department of Cardiology, Peking University Third Hospital (J.Y.), Beijing, the School of Public Health, Changzhi Medical College, Changzhi (X.F., Z.L., P.D.), the School of Public Health, Xi'an Jiaotong University, Xi'an (R.Z., Y.Y.), the School of Public Health and Management, Ningxia Medical University, Yinchuan (Y. Zhang, Y. Zhao, F.W.), the Department of Evidence-Based Medicine, First Hospital of China Medical University, Shenyang (J. Shi, B.Z., B.M.), the Department of Noncommunicable Disease Prevention and Control, Center for Disease Control of Hebei Province, Shijiazhuang (J.Z., J. Sun, W.S.), the School of Public Health, Harbin Medical University, Harbin (M.T.), the Global Health Research Center, Duke Kunshan University, Kunshan (L.L.Y.), and the School of Health Sciences, Wuhan University, Wuhan (L.L.Y.) - all in China; and the Feinberg School of Medicine, Northwestern University, Chicago (D.L.).

Background: Salt substitutes with reduced sodium levels and increased potassium levels have been shown to lower blood pressure, but their effects on cardiovascular and safety outcomes are uncertain.

Methods: We conducted an open-label, cluster-randomized trial involving persons from 600 villages in rural China. The participants had a history of stroke or were 60 years of age or older and had high blood pressure. The villages were randomly assigned in a 1:1 ratio to the intervention group, in which the participants used a salt substitute (75% sodium chloride and 25% potassium chloride by mass), or to the control group, in which the participants continued to use regular salt (100% sodium chloride). The primary outcome was stroke, the secondary outcomes were major adverse cardiovascular events and death from any cause, and the safety outcome was clinical hyperkalemia.

Results: A total of 20,995 persons were enrolled in the trial. The mean age of the participants was 65.4 years, and 49.5% were female, 72.6% had a history of stroke, and 88.4% a history of hypertension. The mean duration of follow-up was 4.74 years. The rate of stroke was lower with the salt substitute than with regular salt (29.14 events vs. 33.65 events per 1000 person-years; rate ratio, 0.86; 95% confidence interval [CI], 0.77 to 0.96; P = 0.006), as were the rates of major cardiovascular events (49.09 events vs. 56.29 events per 1000 person-years; rate ratio, 0.87; 95% CI, 0.80 to 0.94; P<0.001) and death (39.28 events vs. 44.61 events per 1000 person-years; rate ratio, 0.88; 95% CI, 0.82 to 0.95; P<0.001). The rate of serious adverse events attributed to hyperkalemia was not significantly higher with the salt substitute than with regular salt (3.35 events vs. 3.30 events per 1000 person-years; rate ratio, 1.04; 95% CI, 0.80 to 1.37; P = 0.76).

Conclusions: Among persons who had a history of stroke or were 60 years of age or older and had high blood pressure, the rates of stroke, major cardiovascular events, and death from any cause were lower with the salt substitute than with regular salt. (Funded by the National Health and Medical Research Council of Australia; SSaSS ClinicalTrials.gov number, NCT02092090.).
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http://dx.doi.org/10.1056/NEJMoa2105675DOI Listing
September 2021

Coordination of Macro Base Stations for 5G Network with User Clustering.

Sensors (Basel) 2021 Aug 16;21(16). Epub 2021 Aug 16.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

With the increasing amounts of terminal equipment with higher requirements of communication quality in the emerging fifth generation mobile communication network (5G), the energy consumption of 5G base stations (BSs) is increasing significantly, which not only raises the operating expenses of telecom operators but also imposes a burden on the environment. To solve this problem, a two-step energy management method that coordinates 5G macro BSs for 5G networks with user clustering is proposed. The coordination among the communication equipment and the standard equipment in 5G macro BSs is developed to reduce both the energy consumption and the electricity costs. A novel user clustering method is proposed together with Benders decomposition to accelerate the solving process. Simulation results show that the proposed method is computationally efficient and can ensure near-optimal performance, effectively reducing the energy consumption and electricity costs compared with the conventional dispatching scheme.
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http://dx.doi.org/10.3390/s21165501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401326PMC
August 2021

Characteristics and Environmental Response of White Secondary Mineral Precipitate in the Acid Mine Drainage From Jinduicheng Mine, Shaanxi, China.

Bull Environ Contam Toxicol 2021 Aug 21. Epub 2021 Aug 21.

School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, P. R. China.

The study focuses on the white secondary mineral precipitate and its environmental response formed in acid mine drainage (AMD) at Jinduicheng Mine (Shaanxi, China). The mineral composition of white precipitate was characterized by Scanning electron microscopy-energy dispersive spectrometer (SEM-EDS), X-ray photoelectron spectroscopy (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), Inductively coupled plasma-atomic emission spectrometer (ICP-AES), chemical quantitative calculation and PHREEQC software. The white precipitate was a kind of amorphous crystal with the characteristics of a fine powder, and its main elements were O, Al, S, F, OH and SO groups. Moreover, by comparing the mole number of chemical elements, the main mineral composition of the white precipitate was closest to basaluminite. The geochemical simulation result of the PHREEQC software verified that the white precipitate was basaluminite. According to the analysis of water quality characteristics of water samples, basaluminite can reduce the ions content in the AMD and enrich Cu, Ni, Mo, Cr and F ions, showing an excellent self-purification capacity of the water body. These results are helpful to improve the understanding of secondary mineral and its environmental response, and are of great significance for the environmental protection and sustainable development of mining area.
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http://dx.doi.org/10.1007/s00128-021-03355-9DOI Listing
August 2021

Platinum Modulates Redox Properties and 5-Hydroxymethylfurfural Adsorption Kinetics of Ni(OH) for Biomass Upgrading.

Angew Chem Int Ed Engl 2021 10 14;60(42):22908-22914. Epub 2021 Sep 14.

State Key Laboratory of Chem/Bio-Sensing and Chemometrics, Provincial Hunan Key Laboratory for Graphene Materials and Devices, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.

Nickel hydroxide (Ni(OH) ) is a promising electrocatalyst for the 5-hydroxymethylfurfural oxidation reaction (HMFOR) and the dehydronated intermediates Ni(OH)O species are proved to be active sites for HMFOR. In this study, Ni(OH) is modified by platinum to adjust the electronic structure and the current density of HMFOR improves 8.2 times at the Pt/Ni(OH) electrode compared with that on Ni(OH) electrode. Operando methods reveal that the introduction of Pt optimized the redox property of Ni(OH) and accelerate the formation of Ni(OH)O during the catalytic process. Theoretical studies demonstrate that the enhanced Ni(OH)O formation kinetics originates from the reduced dehydrogenation energy of Ni(OH) . The product analysis and transition state simulation prove that the Pt also reduces adsorption energy of HMF with optimized adsorption behavior as Pt can act as the adsorption site of HMF. Overall, this work here provides a strategy to design an efficient and universal nickel-based catalyst for HMF electro-oxidation, which can also be extended to other Ni-based catalysts such as Ni(HCO ) and NiO.
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http://dx.doi.org/10.1002/anie.202109211DOI Listing
October 2021

Effect of phosphogypsum on the properties of magnesium phosphate cement paste with low magnesium-to-phosphate ratio.

Sci Total Environ 2021 Dec 28;798:149262. Epub 2021 Jul 28.

School of Architecture and Planning, Yunnan University, Kunming 650051, China. Electronic address:

The incorporation of phosphogypsum (PG) in magnesium potassium phosphate cement (MPPC) can promote the utilization of PG not only by utilising the phosphate impurity in PG, but also by immobilising the heavy metals with MPPC. This paper investigates the feasibility of the incorporation of PG in preparing MPPC. Both early age properties, including workability and setting time, and hardened properties of compressive strength and microstructure, of PG-incorporated MPPC paste were investigated, and the hydration mechanism was explored. The results indicated that the addition of PG increased the workability of MPPC and extended the setting time of MPPC. However, incorporation of 20% PG slightly reduced the compressive strength because higher PG content led to the loose microstructure. Moreover, the addition of PG did not change the formation of hydration product, while it only reduced the hydration heat. Finally, compared to PG, the concentration of leached heavy metals of MPPC with PG was significantly reduced after 28 days curing.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149262DOI Listing
December 2021

Metformin Alleviates Hepatic Steatosis and Insulin Resistance in a Mouse Model of High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease by Promoting Transcription Factor EB-Dependent Autophagy.

Front Pharmacol 2021 23;12:689111. Epub 2021 Jul 23.

The First Affiliated Hospital, Yunnan Institute of Digestive Disease, Yunnan Clinical Research Center for Digestive Diseases, Kunming Medical University, Kunming, China.

Nonalcoholic fatty liver disease (NAFLD) results from an abnormal accumulation of lipids within hepatocytes, and is commonly associated with obesity, insulin resistance, and hyperlipidemia. Metformin is commonly used to treat type 2 diabetes mellitus and, in recent years, it was found to play a potential role in the amelioration of NAFLD. However, the mechanisms underlying the protective effect of metformin against NAFLD remain largely unknown. Transcription factor EB (TFEB) is a master transcriptional regulator of lysosomal biogenesis and autophagy and, when activated, is effective against disorders of lipid metabolism. However, the role of TFEB in hepatic steatosis is not well understood. In this report, we demonstrate that the activity of TFEB is reduced in the liver of mice fed a high-fat diet. Metformin treatment significantly reverses the activity of TFEB, and the protective effect of metformin against hepatic steatosis and insulin resistance is dependent on TFEB. We show that metformin-induced autophagy is regulated by TFEB, and our findings reveal that TFEB acts as a mediator, linking metformin with autophagy to reverse NAFLD, and highlight that TFEB may be a promising molecular target for the treatment of NAFLD.
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http://dx.doi.org/10.3389/fphar.2021.689111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346235PMC
July 2021

Association of CASC18/miR-20a-3p/TGFB2 ceRNA axis with occult lymph node metastasis in tongue squamous cell carcinoma.

Mol Med 2021 08 6;27(1):85. Epub 2021 Aug 6.

Translational Medicine Centre, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 283 Tongzipo Road, Changsha, 410013, Hunan, People's Republic of China.

Background: Tongue squamous cell carcinoma (TSCC) ranks as the most prevalent malignancy in the oral cavity. TSCC patients with occult lymph node metastasis (OLNM) are thought to be at risk of worse outcome. However, regulatory mechanisms underlying OLNM remain less investigated.

Methods: In the present study, CASC18/miR-20a-3p/TGFB2 axis was identified and evaluated by bioinformatic and qRT-PCR analyses. Effects of CASC18 knockdown on cell migration and invasion were determined by wound healing and transwell assays. Western blot, ELISA, RNA pulldown and luciferase reporter assays were performed for mechanism verification.

Results: CASC18 was identified up-regulating in TSCC tumours, and especially in those from patients with OLNM. Importantly, we found higher CASC18 expression was positively correlated with the presence of OLNM and worse outcome of TSCC patients. Furthermore, we demonstrated that CASC18 knockdown repressed cell migration and invasion through inhibiting epithelial-mesenchymal transition, which could be partly rescued by miR-20a-3p inhibitor. Regarding the molecular mechanism, we further confirmed that CASC18 functioned as a ceRNA to sponge miR-20a-3p to enhanceTGFB2 expression and secretion.

Conclusion: In conclusion, we have reported a novel CASC18/miR-20a-3p/TGFB2 ceRNA axis in OLNM of TSCC. Our findings will contribute to a deeper understanding of the molecular mechanism of OLNM in TSCC, and facilitate the development of diagnostic methods for assisting treatment decision-making.
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http://dx.doi.org/10.1186/s10020-021-00345-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349069PMC
August 2021

Identification of the structural features of quinazoline derivatives as EGFR inhibitors using 3D-QSAR modeling, molecular docking, molecular dynamics simulations and free energy calculations.

J Biomol Struct Dyn 2021 Aug 2:1-16. Epub 2021 Aug 2.

State Key Laboratory of Functions and Applications of Medicinal Plants, College of Basic Medical, Guizhou Medical University, Guizhou, China.

Epidermal growth factor receptor (EGFR) is a promising target for the treatment of different types of malignant tumors. Therefore, a combined molecular modeling study was performed on a series of quinazoline derivatives as EGFR inhibitors. The optimum ligand-based CoMFA and CoMSIA models showed reliable and satisfactory predictability (with R=0.681, R=0.844, R=0.8702 and R=0.643, R=0.874, R=0.6423). The derived contour maps provide structural features to improve inhibitory activity. Furthermore, the contour maps, molecular docking, and molecular dynamics (MD) simulations have good consistency, illustrating that the derived models are reliable. In addition, MD simulations and binding free energy calculations were also carried out to understand the conformational fluctuations at the binding pocket of the receptor. The results indicate that hydrogen bond, hydrophobic and electrostatic interactions play significant roles on activity and selectivity. Furthermore, amino acids Val31, Lys50, Thr95, Leu149 and Asp160 are considered as essential residues to participate in the ligand-receptor interactions. Overall, this work would offer reliable theoretical basis for future structural modification, design and synthesis of novel EGFR inhibitors with good potency.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2021.1956591DOI Listing
August 2021

The Effect of Ultrahigh Altitude on the Mental Health of Civil Servants in Western China Based on Propensity Score Matching.

High Alt Med Biol 2021 Jul 29. Epub 2021 Jul 29.

Sichuan Provincial Center for Mental Health, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.

Wang, Luyao, Bo Zhou, Chenghui Yang, Shuya Pan, Yulan Huang, and Jinyu Wang. The effect of ultrahigh altitude on the mental health of civil servants in western China based on propensity score matching. 00:000-000, 2021. This study aims to analyze the net effect of ultrahigh altitude on the mental health of civil servants in western China after adjusting for sociodemographic factors. A cross-sectional study was performed to survey the mental health of 2,939 civil servants working at an altitude of more than 1,500 m in 13 areas of the Tibetan Qiang Autonomous Prefecture of Ngawa using the Insomnia Severity Index Questionnaire, 7-item Generalized Anxiety Disorder Scale, and Patient Health Questionnaire-9. Ultrahigh altitude refers to an area above 3,500 m above sea level, which may have an impact on the sleep and mood of residents. Therefore, our research was divided into two groups based on altitude (ultrahigh altitude >3,500 m; high altitude = 1,500-3,400 m). Propensity score matching (PSM) was used to control for sociodemographic factors and compare the differences in mental health between the two groups. After kernel matching, the mean bias of the covariates was reduced from 21.6 to 1.8. The severity of insomnia, depression, and anxiety in civil servants at ultrahigh altitudes was still significantly greater than that in civil servants at high altitudes after controlling for sociodemographic factors, and the average treatment effects on the treated were 1.39, 1.35, and 0.80, respectively; the results were significant ( < 0.01). PSM regression analysis further showed that for every 100 m increase in altitude, the severity of anxiety, depression, and insomnia increased by 0.042 points ( < 0.001), 0.063 points ( < 0.001), and 0.070 points ( < 0.001), respectively, all of which were higher than those obtained with ordinary least squares regression. Ultrahigh altitude significantly increases the severity of insomnia, depression, and anxiety after adjusting for sociodemographic factors.
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http://dx.doi.org/10.1089/ham.2020.0086DOI Listing
July 2021

Periodic limb movements in patients with obstructive sleep apnea syndrome.

Sci Rep 2021 07 28;11(1):15341. Epub 2021 Jul 28.

Department of Psychosomatics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

The aim of the study was to assess the factors associated with periodic limb movements during sleep (PLMS) among obstructive sleep apnea syndrome (OSAS) patients and identify the role of PLMS in patients with OSAS. 303 adult patients with OSAS were included in the study. All patients completed physical examination, Epworth sleepiness scale (ESS), and polysomnography. Diagnosis of PLMS was made if the periodic leg movements index (PLMI) was ≥ 15. Chi-square test, ANOVA, univariate and multivariate logistic regression analyses were conducted to identify factors associated with PLMS among OSAS patients. Statistical analyses were performed with SPSS 26.0 for mac. Statistically significant difference was considered if P value < 0 .05. Among the 303 adult patients with OSAS, 98 patients had significant PLMS and the other 205 had no significant PLMS. Compared with OSAS patients without PLMS, OSAS patient with PLMS were older, had shorter REM duration and greater apnea-hypopnea index (AHI) (P < 0.05). The study suggests that PLMS is a matter of concern among patients with OSAS. A better understanding of the role of PLMS among OSAS patients could be useful in better recognition, intervention and treatment of OSAS.
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http://dx.doi.org/10.1038/s41598-021-95018-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319330PMC
July 2021

Development and Mechanistic Studies of the Iridium-Catalyzed C-H Alkenylation of Enamides with Vinyl Acetates: A Versatile Approach for Ketone Functionalization.

Angew Chem Int Ed Engl 2021 09 16;60(38):20926-20934. Epub 2021 Aug 16.

Department of Chemistry, University of Chicago, Chicago, IL, 60637, USA.

Ketone functionalization is a cornerstone of organic synthesis. Herein, we describe the development of an intermolecular C-H alkenylation of enamides with the feedstock chemical vinyl acetate to access diverse functionalized ketones. Enamides derived from various cyclic and acyclic ketones reacted efficiently, and a number of sensitive functional groups were tolerated. In this iridium-catalyzed transformation, two structurally and electronically similar alkenes-enamide and vinyl acetate-underwent selective cross-coupling through C-H activation. No reaction partner was used in large excess. The reaction is also pH- and redox-neutral with HOAc as the only stoichiometric by-product. Detailed experimental and computational studies revealed a reaction mechanism involving 1,2-Ir-C migratory insertion followed by syn-β-acetoxy elimination, which is different from that of previous vinyl acetate mediated C-H activation reactions. Finally, the alkenylation product can serve as a versatile intermediate to deliver a variety of structurally modified ketones.
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http://dx.doi.org/10.1002/anie.202107331DOI Listing
September 2021

Protocol for the economic evaluation of the China Salt Substitute and Stroke Study (SSaSS).

BMJ Open 2021 07 20;11(7):e045929. Epub 2021 Jul 20.

The George Institute for Global Health, University of New South Wales, Sydney, New South Wales, Australia.

Introduction: Cardiovascular diseases (CVDs) are the leading causes of death and disability worldwide. Reducing dietary salt consumption is a potentially cost-effective way to reduce blood pressure and the burden of CVD. To date, economic evidence has focused on sodium reduction in food industry or processed food with blood pressure as the primary outcome. This study protocol describes the planned within-trial economic evaluation of a low-sodium salt substitute intervention designed to reduce the risk of stroke in China.

Methods And Analyses: The economic evaluation will be conducted alongside the Salt Substitute and Stroke Study: a 5-year large scale, cluster randomised controlled trial. The outcomes of interest are quality of life measured using the EuroQol-5-Dimensions and major adverse cardiovascular events. Costs will be estimated from a healthcare system perspective and will be sought from the routinely collected data available within the New Rural Cooperative Medical Scheme. Cost-effectiveness and cost-utility analyses will be conducted, resulting in the incremental cost-effectiveness ratio expressed as cost per cardiovascular event averted and cost per quality-adjusted life year gained, respectively.

Ethics And Dissemination: The trial received ethics approval from the University of Sydney Ethics Committee (2013/888) and Peking University Institutional Review Board (IRB00001052-13069). Informed consent was obtained from each study participant. Findings of the economic evaluation will be published in a peer-reviewed journal and presented at international conferences.

Trial Registration Number: ClinicalTrials.gov Registry (NCT02092090).
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http://dx.doi.org/10.1136/bmjopen-2020-045929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292808PMC
July 2021

Significance of LINC00460 in the progression and prognosis in digestive tract tumors.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Jun;46(6):628-636

Medical Research Center, Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014, China.

The long intergic non-protein coding RNA 460 (LINC00460) is abnormally highly expressed in gastrointestinal tumors and plays an important role in promoting tumor formation and development. LINC00460 is mainly distributed in cytoplasm and has many abnormal gene variants of single nucleotide polymorphism in tumors. LINC00460 can promote the proliferation, metastasis, angiogenesis, radiotherapy and chemotherapy resistance, inhibit the apoptosis of tumor cells, and further promote the malignant progression of tumors via involving in chromatin state maintenance, methylation modification, endogenous competition and transcriptional regulation. It may serve as a valuable tumor marker and therapeutic target.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200975DOI Listing
June 2021

Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer.

Nat Commun 2021 07 16;12(1):4362. Epub 2021 Jul 16.

Department of Medicine, Samuel Oschin Cancer Center, Cedars-Sinai Medical Center, Los Angeles, CA, USA.

Squamous cell carcinomas (SCCs) comprise one of the most common histologic types of human cancer. Transcriptional dysregulation of SCC cells is orchestrated by tumor protein p63 (TP63), a master transcription factor (TF) and a well-researched SCC-specific oncogene. In the present study, both Gene Set Enrichment Analysis (GSEA) of SCC patient samples and in vitro loss-of-function assays establish fatty-acid metabolism as a key pathway downstream of TP63. Further studies identify sterol regulatory element binding transcription factor 1 (SREBF1) as a central mediator linking TP63 with fatty-acid metabolism, which regulates the biosynthesis of fatty-acids, sphingolipids (SL), and glycerophospholipids (GPL), as revealed by liquid chromatography tandem mass spectrometry (LC-MS/MS)-based lipidomics. Moreover, a feedback co-regulatory loop consisting of SREBF1/TP63/Kruppel like factor 5 (KLF5) is identified, which promotes overexpression of all three TFs in SCCs. Downstream of SREBF1, a non-canonical, SCC-specific function is elucidated: SREBF1 cooperates with TP63/KLF5 to regulate hundreds of cis-regulatory elements across the SCC epigenome, which converge on activating cancer-promoting pathways. Indeed, SREBF1 is essential for SCC viability and migration, and its overexpression is associated with poor survival in SCC patients. Taken together, these data shed light on mechanisms of transcriptional dysregulation in cancer, identify specific epigenetic regulators of lipid metabolism, and uncover SREBF1 as a potential therapeutic target and prognostic marker in SCC.
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http://dx.doi.org/10.1038/s41467-021-24656-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285542PMC
July 2021

Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway.

Pathol Oncol Res 2021 7;27:643376. Epub 2021 Apr 7.

Key Laboratory of Cell Biology of Ministry of Public Health, and Key Laboratory of Medical Cell Biology of Ministry of Education, China Medical University, Shenyang, China.

B-cell acute lymphoblastic leukemia (B-ALL) is the common type of blood cancer. Although the remission rate has increased, the current treatment options for B-ALL are usually related to adverse reactions and recurrence, so it is necessary to find other treatment options. G protein signaling modulator 1 (GPSM1) is one of several factors that affect the basic activity of the G protein signaling system, but its role in B-ALL has not yet been clarified. In this study, we analyzed the expression of GPSM1 in the Oncomine database and found that the GPSM1 levels were higher in B-ALL cells than in peripheral blood mononuclear cells (PBMCs). Analyses of the Gene Expression Profiling Interactive Analysis (GEPIA) demonstrated that patients with high GPSM1 levels had shorter survival times than those with low levels. Additionally, gene set enrichment analysis (GSEA) suggested that GPSM1 was positively correlated with proliferation, G protein-coupled receptor (GPCR) ligand binding, Gαs signaling and calcium signaling pathways. In further experiments, GPSM1 was found to be highly expressed in Acute lymphoblastic leukemia (ALL) cell lines, and downregulation of GPSM1 inhibited proliferation and promoted cell cycle arrest and apoptosis in BALL-1 and Reh cells. Moreover, knockdown of GPSM1 suppressed ADCY6 and RAPGEF3 expression in BALL-1 and Reh cells. Furthermore, we reported that GPSM1 regulated JNK expression via ADCY6-RAPGEF3. The present study demonstrates that GPSM1 promotes tumor growth in BALL-1 and Reh cells by modulating ADCY6-RAPGEF3-JNK signaling.
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http://dx.doi.org/10.3389/pore.2021.643376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262160PMC
April 2021

Adjusting the Covalency of Metal-Oxygen Bonds in LaCoO by Sr and Fe Cation Codoping to Achieve Highly Efficient Electrocatalysts for Aprotic Lithium-Oxygen Batteries.

ACS Appl Mater Interfaces 2021 Jul 9;13(28):33133-33146. Epub 2021 Jul 9.

College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, 1#, Dongsanlu, Erxianqiao, Chengdu 610059, Sichuan, P. R. China.

Developing high-efficiency dual-functional catalysts to promote oxygen electrode reactions is critical for achieving high-performance aprotic lithium-oxygen (Li-O) batteries. Herein, Sr and Fe cation-codoped LaCoO perovskite (LaSrCoFeO, LSCFO) porous nanoparticles are fabricated as promising electrocatalysts for Li-O cells. The results demonstrate that the LSCFO-based Li-O batteries exhibit an extremely low overpotential of 0.32 V, ultrahigh specific capacity of 26 833 mA h g, and superior long-term cycling stability (200 cycles at 300 mA g). These prominent performances can be partially attributed to the existence of abundant coordination unsaturated sites caused by oxygen vacancies in LSCFO. Most importantly, density functional theory (DFT) calculations reveal that codoping of Sr and Fe cations in LaCoO results in the increased covalency of Co 3d-O 2p bonds and the transition of Co from an ordinary low-spin state to an intermediate-spin state, eventually resulting in the transformation from nonconductor LCO to metallic LSCFO. In addition, based on the theoretical calculations, it is found that the inherent adsorption capability of LSCFO toward the LiO intermediate is reduced due to the increased covalency of Co 3d-O 2p bonds, leading to the formation of large granule-like LiO, which can be effectively decomposed on the LSCFO surface during the charging process. Notably, this work demonstrates a unique insight into the design of advanced perovskite oxide catalysts via adjusting the covalency of transition-metal-oxygen bonds for high-performance metal-air batteries.
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http://dx.doi.org/10.1021/acsami.1c08586DOI Listing
July 2021

Deciphering the effects of PYCR1 on cell function and its associated mechanism in hepatocellular carcinoma.

Int J Biol Sci 2021 1;17(9):2223-2239. Epub 2021 Jun 1.

Department of pathology, Affiliated hospital of Guilin Medical University, Guilin, 541001, Guangxi, China.

Overexpression of pyrroline-5-carboxylate reductase 1 (PYCR1) has been associated with the development of certain cancers; however, no studies have specifically examined the role of PYCR1 in hepatocellular carcinoma (HCC). Based on The Cancer Genome Atlas expression array and meta-analysis conducted using the Gene Expression Omnibus database, we determined that was upregulated in HCC compared to adjacent nontumor tissues (P < 0.05). These data were verified using quantitative real-time polymerase chain reaction, western blotting, and immunohistochemistry analysis. Additionally, patients with low PYCR1 expression showed a higher overall survival rate than patients with high expression. Furthermore, PYCR1 overexpression was associated with the female sex, higher levels of alpha-fetoprotein, advanced clinical stages (III and IV), and a younger age (< 45 years old). Silencing of inhibited cell proliferation, invasive migration, epithelial-mesenchymal transition, and metastatic properties in HCC and Using RNA sequencing and bioinformatics tools for data-dependent network analysis, we found binary relationships among PYCR1 and its interacting proteins in defined pathway modules. These findings indicated that PYCR1 played a multifunctional role in coordinating a variety of biological pathways involved in cell communication, cell proliferation and growth, cell migration, a mitogen-activated protein kinase cascade, ion binding, . The structural characteristics of key pathway components and PYCR1-interacting proteins were evaluated by molecular docking, and hotspot analysis showed that better affinities between PYCR1 and its interacting molecules were associated with the presence of arginine in the binding site. Finally, a candidate regulatory microRNA, miR-2355-5p, for mRNA was discovered in HCC. Overall, our study suggests that PYCR1 plays a vital role in HCC pathogenesis and may potentially serve as a molecular target for HCC treatment.
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http://dx.doi.org/10.7150/ijbs.58026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241733PMC
June 2021
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