Publications by authors named "Bo Zhao"

1,135 Publications

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Mechanically active small intestinal submucosa hydrogel for accelerating chronic wound healing.

J Mater Chem B 2022 Aug 5. Epub 2022 Aug 5.

Beijing Biosis Healing Biological Technology Co., Ltd, Beijing, 102600, China.

The treatment of chronic wounds is still a challenge worldwide. Here, inspired by mechanically induced embryonic wound healing, we design a mechanically active small intestinal submucosa based hydrogel (SIS-PNIPAm). The mechanical activity, biocompatibility, and bioactivity (angiogenesis and immunoregulation) of the SIS-PNIPAm hydrogel enable the fast healing of diabetic rat full-thickness wounds.
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http://dx.doi.org/10.1039/d2tb01355jDOI Listing
August 2022

Quantitative CT evaluation after two cycles of induction chemotherapy to predict prognosis of patients with locally advanced oesophageal squamous cell carcinoma before undergoing definitive chemoradiotherapy/radiotherapy.

Eur Radiol 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/ Beijing), Department of Radiology, Peking University Cancer Hospital & Institute, No. 52, Fucheng Road, Hai Dian District, Beijing, 100142, China.

Objective: To investigate the performance of quantitative CT analysis in predicting the prognosis of patients with locally advanced oesophageal squamous cell carcinoma (ESCC) after two cycles of induction chemotherapy before definitive chemoradiotherapy/radiotherapy.

Methods: A total of 110 patients with locally advanced ESCC were retrospectively analysed. Baseline chest CT and CT after two cycles of induction chemotherapy were analysed. A multivariate Cox proportional-hazard regression model was used to identify independent prognostic markers for survival analysis. Then, a CT scoring system was established. Time-dependent receiver operating characteristic (ROC) curve analysis and the Kaplan-Meier method were employed for analysing the prognostic value of the CT scoring system.

Results: Body mass index, treatment strategy, change ratios of thickness (ΔTH), CT value of the primary tumour (ΔCTV) and the short diameter (ΔSD-LN), and the presence of an enlarged small lymph node (ESLN) after two cycles of chemotherapy were noted as independent factors for predicting overall survival (OS). The specificity of the presence of ESLN for death after 12 months was up to 100%. Areas under the curve value of the CT scoring system for predicting OS and progression-free survival (PFS) were higher than that of the RECIST (p < 0.05). Responders had significantly longer OS and PFS than non-responders.

Conclusion: Quantitative CT analysis after two cycles of induction chemotherapy could predict the outcome of locally advanced ESCC patients treated with definitive chemoradiotherapy/radiotherapy. The CT scoring system could contribute to the development of an appropriate strategy for patients with locally advanced ESCC.

Key Points: • Quantitative CT evaluation after two cycles of induction chemotherapy can predict the long-term outcome of locally advanced oesophageal cancer treated with definitive chemoradiotherapy/radiotherapy. • A CT scoring system provides valuable imaging support for indicating the prognosis at the early stage of therapy. • Quantitative CT evaluation can assist clinicians in personalising treatment plans.
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http://dx.doi.org/10.1007/s00330-022-08994-yDOI Listing
August 2022

The role of PP2A /NLRP3 signaling pathway in ambient particulate matter 2.5 induced lung injury.

Chemosphere 2022 Aug 1;307(Pt 2):135794. Epub 2022 Aug 1.

Department of Toxicology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China; Hebei Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, 050017, Hebei, China. Electronic address:

Ambient particulate matter 2.5 (PM) exposure has been linked to pulmonary fibrosis. However, the key signaling pathways remained unclear. In the present study, we applied a mouse model with myeloid-specific deletion of Ppp2r1a gene (encoding protein phosphatase 2 A (PP2A) A subunit) to identify the key signaling pathways involved in PM-induced pulmonary fibrosis. PP2A Aα homozygote mice and matched wild-type (WT) littermates were exposed to filtered air (FA), unfiltered air (UA), and concentrated PM (CA) in a real-ambient PM exposure system for 8 weeks and 16 weeks, respectively. The mice exposed to PM displayed a progressive inflammation and pulmonary fibrosis. Moreover, the expressions of NLRP3, pro-caspase-1, caspase-1, ASC and IL-1β were increased in mice lung following PM exposure, indicating PM exposure caused pulmonary inflammation by the NLRP3 pathways activation. Furthermore, the effects of PM exposure on pulmonary inflammation, pulmonary fibrosis, oxidative stress, and pulmonary function damage were significantly enhanced in PP2A mice compared to WT mice, indicating the role of PP2A in the regulation of pulmonary injury induced by PM exposure. In vitro study confirmed that PP2A was involved in the PM-induced inflammation response and NLRP3 inflammasome activation. Importantly, we identified PP2A regulated the activation of NLRP3 pathways by direct dephosphorylating IRE1α in response to PM exposure. Taken together, our results demonstrated that PP2A-IRE1α-NLRP3 signaling pathway played a crucial role in regulating the inflammation response, triggering the lung fibrogenesis upon PM exposure. Our findings provide new insights into regulatory role of PP2A in human diseases upon the PM exposure.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135794DOI Listing
August 2022

Guided bone regeneration with extracellular matrix scaffold of small intestinal submucosa membrane.

J Biomater Appl 2022 Aug 4:8853282221114450. Epub 2022 Aug 4.

Beijing Biosis Healing Biological Technology Co, Ltd, Beijing, China.

Guided bone regeneration (GBR) is a promising strategy for repairing bone defects using bioactive membranes. In this study, a new type of GBR membrane based on the small intestinal submucosa (SIS) was created, and its surface structure, cytological characteristics, and bone defect repair ability were compared with commonly used membranes. Our results show that compared to the Heal-all and Dentium membranes, the SIS membrane has an asymmetric structure that does not affect the proliferation of bone marrow mesenchymal stem cells (BMSCs). Instead, it increased their formation of calcium nodules and expression of bone morphogenetic protein-2 (BMP-2), alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteopontin (OPN). Six weeks after their insertion into a rat calvarial defect model, increased bone growth was observed in the SIS membrane group. Our results indicate that the SIS membrane has good biocompatibility and is more effective in promoting early bone formation than existing membranes. Given the wide range of source materials and simple preparation processes available, SIS membrane is a promising candidate for guided bone regeneration.
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http://dx.doi.org/10.1177/08853282221114450DOI Listing
August 2022

Biliverdin Reductase A Protects Lens Epithelial Cells against Oxidative Damage and Cellular Senescence in Age-Related Cataract.

Oxid Med Cell Longev 2022 19;2022:5628946. Epub 2022 Jul 19.

Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Age-related cataract (ARC) is the common cause of blindness globally. Reactive oxygen species (ROS), one of the greatest contributors to aging process, leads to oxidative damage and senescence of lens epithelial cells (LECs), which are involved in the pathogenesis of ARC. Biliverdin reductase A (BVRA) has ROS-scavenging ability by converting biliverdin (BV) into bilirubin (BR). However, little is known about the protective effect of BVRA against ARC. In the present study, we measured the expression level of BVRA and BR generation in human samples. Then, the antioxidative property of BVRA was compared between the young and senescent LECs upon stress condition. In addition, we evaluated the effect of BVRA on attenuating HO-induced premature senescence in LECs. The results showed that the mRNA expression level of BVRA and BR concentration were decreased in both LECs and lens cortex of age-related nuclear cataract. Using the RNA interference technique, we found that BVRA defends LECs against oxidative stress via (i) restoring mitochondrial dysfunction in a BR-dependent manner, (ii) inducing heme oxygenase-1 (HO-1) expression directly, and (iii) promoting phosphorylation of ERK1/2 and nuclear delivery of nuclear factor erythroid 2-related factor 2 (Nrf2). Intriguingly, the antioxidative effect of BVRA was diminished along with the reduced BR concentration and repressed nuclear translocation of BVRA and Nrf2 in senescent LECs, which would be resulted from the decreased BVRA activity and impaired nucleocytoplasmic trafficking. Eventually, we confirmed that BVRA accelerates the G1 phase transition and prevents against HO-induced premature senescence in LECs. In summary, BVRA protects LECs against oxidative stress and cellular senescence in ARC by converting BV into BR, inducing HO-1 expression, and activating the ERK/Nrf2 pathway. This trial is registered with ChiCTR2000036059.
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http://dx.doi.org/10.1155/2022/5628946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325611PMC
August 2022

Resonant Control of Elastic Collisions between ^{23}Na^{40}K Molecules and ^{40}K Atoms.

Phys Rev Lett 2022 Jul;129(3):033401

Hefei National Research Center for Physical Sciences at the Microscale and School of Physical Sciences, University of Science and Technology of China, Hefei 230026, China; Shanghai Research Center for Quantum Science and CAS Center for Excellence in Quantum Information and Quantum Physics, University of Science and Technology of China, Shanghai 201315, China; and Hefei National Laboratory, University of Science and Technology of China, Hefei 230088, China.

We have demonstrated the resonant control of the elastic scattering cross sections in the vicinity of Feshbach resonances between ^{23}Na^{40}K molecules and ^{40}K atoms by studying the thermalization between them. The elastic scattering cross sections vary by more than 2 orders of magnitude close to the resonance, and can be well described by an asymmetric Fano profile. The parameters that characterize the magnetically tunable s-wave scattering length are determined from the elastic scattering cross sections. The observation of resonantly controlled elastic scattering cross sections opens up the possibility to study strongly interacting atom-molecule mixtures and improve our understanding of the complex atom-molecule Feshbach resonances.
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http://dx.doi.org/10.1103/PhysRevLett.129.033401DOI Listing
July 2022

Failure Mechanism of the Fire Control Computer CPU Board inside the Tank under Transient Shock: Finite Element Simulations and Experimental Studies.

Materials (Basel) 2022 Jul 21;15(14). Epub 2022 Jul 21.

College of Mechatronic Engineering, North University of China, Taiyuan 030051, China.

The electronic components inside a main battle tank (MBT) are the key components for the tank to exert its combat effectiveness. However, breakdown of the inner electronic components can easily occur inside the MBT due to the strong transient shock and large vibration during artillery fire. As a typical key electronic component inside an MBT, the fault mechanism and fault patterns of the CPU board of the fire control computer (FCC) are discussed through numerical simulation and experimental research. An explicit nonlinear dynamic analysis is performed to study the vibration features and fault mechanism under instantaneous shock load. By using finite element modal analysis, the first six nature frequencies of the CPU board are calculated. Meanwhile, curves of stress-frequency and strain-frequency of the CPU board under different harmonic loads are obtained, which are applied to further identify the peak response of the structure. Validation of the finite element model and simulation results are performed by comparing those obtained from the modal with experiments. Based on the dynamic simulation and experimental analysis, fault patterns of CPU board are discussed, and some optimization suggestions were proposed. The results shown in this work can provide a potential technical basis and reference for the optimization design of the electronic components that are commonly used in the modern weapon equipment and wartime support.
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http://dx.doi.org/10.3390/ma15145070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318559PMC
July 2022

A visualized pulmonary arterial thrombus by using a modified echocardiographic view in an intermediate-risk acute pulmonary embolism patient: A case report.

Clin Case Rep 2022 Jul 19;10(7):e6105. Epub 2022 Jul 19.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular disease, Department of Cardiology, Tianjin Institute of Cardiology Second Hospital of Tianjin Medical University Tianjin China.

Acute pulmonary embolism (APE) is a life-threatening disease with nonspecific clinical signs and symptoms. Rapid and accurate diagnosis is crucial for the clinical management of patients with acute pulmonary embolism. A recommended echocardiography view may be of further help in the diagnosis and evaluation of the change in thrombosis and treatment. We reported a case of a 74-year-old man with a 12-day history of decreased exercise capacity and dyspnea. The patient was diagnosed with intermediate-risk APE as several pulmonary emboli in pulmonary artery were seen in multidetector computed tomographic pulmonary angiography with normal blood pressure and echocardiographic right ventricular overload. And we found a pulmonary artery clot in the right pulmonary artery through transthoracic echocardiography. After 11-days anticoagulation, the patient underwent a reassessment, showed a decrease in RV diameter and pulmonary artery thrombus. This case highlights the significant role that echocardiography played in a patient who presented pulmonary embolism with a stable hemodynamic situation and normal blood pressure. The modified echocardiographic view could provide correct diagnosis by identifying the clot size and location visually. Knowledge of the echocardiography results of APE would aid the diagnosis.
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http://dx.doi.org/10.1002/ccr3.6105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295681PMC
July 2022

Research on Percolation Characteristics of a JANUS Nanoflooding Crude Oil System in Porous Media.

ACS Omega 2022 Jul 27;7(27):23107-23114. Epub 2022 Jun 27.

Daqing Oil Field Co., Ltd. No. 6 Oil Production Plant, Daqing 163000, China.

According to the research status of low-permeability reservoir development, in order to find an intelligent and efficient displacement method, a Janus smart nanocapsule (embedding nanomaterials with surfactant function into the polymer) is developed in this paper. There are two kinds of phase fluids in the migration of porous media, the initial Janus intelligent microcapsule slow-release liquid (dissolved and undissolved AP-g-PNIPAAM and Janus functional particle ternary dispersion) and the later AP-g-PNIPAAM and Janus functional particle binary dispersion. Comprehensively, using the indoor oil displacement experiment, the seepage characteristics of the Janus smart nanocapsule (JSNC) in porous media are studied, and their macro and micro oil displacement mechanisms are revealed. Research shows that Janus intelligent microcapsules have good mobility control ability in low-permeability heterogeneous reservoirs. The displacement performance of stepped differential pressure shows that the displacement medium can expand the swept volume. The research results presented can show that the JSNC oil displacement system has great application potential for the development of low-permeability reservoirs.
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http://dx.doi.org/10.1021/acsomega.2c00547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280955PMC
July 2022

Efficacy and safety of thoracoscopic resection for early-stage non-small cell lung cancer.

Am J Transl Res 2022 15;14(6):4024-4032. Epub 2022 Jun 15.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, China.

Objective: To explore the efficacy and safety of thoracoscopic resection for early-stage non-small cell lung cancer (NSCLC).

Methods: A total of 110 patients with early-stage NSCLC admitted to Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology between August 2017 and December 2019 were enrolled and retrospectively analysed. Among them, 60 patients receiving thoracoscopic resection for lung cancer (LC) were assigned to the research group (Res group) and another 50 patients treated with routine open radical resection for LC were included in the control group (Con group). The following items of the two groups were evaluated and compared: treatment efficacy, operation indexes, VAS score, lung function, lung capacity, complications, 2-year tumour-free survival rate, 2-year survival rate, and quality of life (QoL).

Results: The Res group showed significantly better efficacy, and lower incidence of complications and VAS score than the Con group. In addition, patients in the Res group experienced less intraoperative blood loss, earlier anal exhaust, shorter hospital stay and indwelling time of drainage tube as well as less drainage volume. Furthermore, better recovery in pulmonary function and lung capacity, and significantly higher 2-year tumour-free survival rate, 2-year survival rate as well as postoperative QoL were noted in the Res group compared with the Con group.

Conclusion: Thoracoscopic resection for LC is effective in the treatment of patients with early-stage NSCLC. It can substantially shorten the hospital stay and indwelling time of drainage tube and reduce drainage volume and blood loss, with high safety.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274577PMC
June 2022

Understory plant removal counteracts tree thinning effect on soil respiration in a temperate forest.

Glob Chang Biol 2022 Jul 14. Epub 2022 Jul 14.

Research Center of Forest Management Engineering of State Forestry and Grassland Administration, Beijing Forestry University, Beijing, China.

Elucidating the response mechanism of soil respiration (Rs) to silvicultural practices is pivotal to evaluating the effects of management practices on soil carbon cycling in planted forest ecosystems. However, as common management practices, how thinning, understory plant removal, and their interactions affect Rs and its autotrophic and heterotrophic components (Ra and Rh) remains unclear. Therefore, we investigated Rs, Ra and Rh by the trenching method from 2011 to 2015 in a Pinus tabuliformis plantation in northern China, subjecting to four treatments (intact control plots [CK], thinning [T], understory removal [UR], and thinning with understory removal [TUR]). Mean annual Rs was significantly increased by thinning (by 15.3%), whereas decreased by UR (by 17.4%), compared with CK. These variations in Rs were mainly attributed to changes in Ra. The increments of Ra were caused by the enhanced growth of fine root biomass after thinning. However, UR led to lower Ra compared with CK (p < .05), indicating that understory growth is inadequate to compensate for the decreased respiring root biomass induced by understory removal. Rs was unchanged between TUR and the intact control plot due to the opposite effects of thinning and UR on the Ra. Changes in Rh exhibited no significant differences among the treatments, partly because of the stable microbial biomass carbon (MBC) and forest floor mass (litter and fine woody debris). No interaction effect between thinning and understory removal was detected on Rs, Ra, and Rh. The lowest temperature sensitivity (Q ) value of Ra was found in CK. This study highlights the necessity of incorporating understory plant effects on soil CO efflux in assessing forest management practices on soil carbon cycling.
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http://dx.doi.org/10.1111/gcb.16337DOI Listing
July 2022

Dexmedetomidine infusion for emergence coughing prevention in patients undergoing an endovascular interventional procedure: A randomized dose-finding trial.

Eur J Pharm Sci 2022 Jul 8;177:106230. Epub 2022 Jul 8.

Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address:

Objective: Dexmedetomidine has been introduced in emergence coughing, agitation, and shivering prevention. This study aimed to investigate the optimal dose of dexmedetomidine for emergence cough prophylaxis.

Methods: In this randomized, double-blinded, and prospective trial, 356 patients scheduled for an endovascular interventional procedure were randomly assigned to 0.3 (D 0.3), 0.4 (D 0.4), 0.5 (D 0.5), and 0.6 (D 0.6) μg·kg·h dexmedetomidine rate, or saline control (C), from anesthesia induction until the end of surgery. The primary outcomes measured were cough grade and frequency. Additionally, groups were compared according to mean arterial pressure (MAP), heart rate, agitation, shivering, postoperative nausea and vomiting (PONV), extubation time, sedation scores, and postoperative first night sleep quality (secondary outcomes).

Results: A total of 351 patients were included in the analysis. The respective incidences of D 0.3, D 0.4, and D 0.5 versus C group were: 78.6%, 68.6%, 53.4% and 42.9% vs 89.7% for cough (p = 0.002, p < 0.001, and p < 0.001 between group D 0.4, D 0.5 and D 0.6 vs C, respectively); 30%, 27.1%, 20.5%, 15.7% vs 44.1% for agitation (p = 0.04, p = 0.003, and p < 0.001 between group D 0.4, D 0.5 and D 0.6 vs C, respectively); 8.6%, 7.1%, 6.8%, 5.7% vs 22.1% for shivering (p = 0.027, p = 0.013, p = 0.01, and p = 0.01 between D 0.3, D 0.4, D 0.5 and D 0.6 vs C, respectively); and 52.9%, 57.1%, 42.5%, 44.3% vs 61.8% for poor sleep quality (p = 0.02 and p = 0.04 between group D 0.5 and D 0.6 vs C, respectively). D 0.4, D 0.5 and D 0.6 showed lower MAP during extubation, compared with the C group. Also, D 0.5 and D 0.6 presented a slight delay in extubation (3.1 and 3.3 min longer than C; p = 0.002 and p < 0.001, respectively). Meanwhile, the frequency of atropine, vasopressor administration, PONV and dizziness were similar to the control.

Conclusions: Both 0.5 and 0.6 μg·kg·h dexmedetomidine infusion rates effectively mitigated emergence coughing with prolonged extubation time, besides sleep disturbance. D 0.4, D 0.5, and D 0.6 reduced agitation and sustained hemodynamic stability. Finally, the four doses applied were effective in shivering attenuation.
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http://dx.doi.org/10.1016/j.ejps.2022.106230DOI Listing
July 2022

Assessing Knowledge, Preventive Practices, and Depression Among Chinese International Students and Local Korean Students in South Korea During the COVID-19 Pandemic: An Online Cross-Sectional Study.

Front Psychiatry 2022 21;13:920887. Epub 2022 Jun 21.

Centre for Health Management and Policy Research, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.

Depression among university students and international university students is an increasing problem globally. This study aimed to clarify the differences on the conditions and determinants of the knowledge, preventive practices and depression of the Chinese international students and local Korean students in South Korea during the COVID-19 pandemic. An online cross-sectional questionnaire including general demographic characteristics, COVID-19-related knowledge, preventive practice, and the Patient Health Questionnaire (PHQ-9) was applied from March 23 to April 22, 2020. A total of 533 university students (171 Chinese international students and 362 local South Korean students) were included in the study. The majority of both Chinese international students and local South Korean students had a good comprehension of COVID-19. Chinese international students in South Korea showed better preventive practice than local Korean students, while the proportion of moderate to severe depression of Chinese international students was relatively higher (28.07%) than that of local Korean students (22.38%). Determinants of depression of Chinese international students in South Korea were information satisfaction, likelihood of survival after infection, symptoms of a cough and feelings of discrimination, while for local Korean students were gender, educational level, family, suspected symptoms, self-assessed physical health status, COVID-19 detection, population contact history and online sources of information. These results could be used as a reference for decreasing the depressive symptoms among the university students.
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http://dx.doi.org/10.3389/fpsyt.2022.920887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258509PMC
June 2022

Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9.

Front Oncol 2022 23;12:837248. Epub 2022 Jun 23.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Tumor metastasis is still an insurmountable obstacle in tumor treatment. Lung cancer represents one of the most common malignancies with high morbidity worldwide. hnRNPA1 has been reported to be involved in the regulation of tumor metastasis, while its specific role in tumor metastasis seems to be controversial and its molecular mechanism in lung cancer metastasis remains to be further elucidated. In this study, we confirmed that knockdown of the hnRNPA1 led to enhanced migration, invasion and EMT transition in lung cancer cells. Bioinformatics analysis of the GSE34992 dataset revealed that hnRNPA1 may regulate the alternative splicing (AS) of LAS1L exon 9. Further AGE assays and RIP assays revealed that hnRNPA1 can directly bind to the LAS1L pre-mRNA to inhibit the splicing of LAS1L exon 9. The RNA pull-down assays showed that hnRNPA1 can specifically bind to the two sites (UAGGGU(WT1) and UGGGGU(WT3)) of LAS1L Intron 9. Further Transwell assays indicated that the expression ratio of LAS1L-L/LAS1L-S regulated by hnRNPA1 can further promote the migration, invasion and EMT transition in lung cancer cells. Moreover, hnRNPA1 expression showed significant heterogeneity in lung cancer tissues, which may contain new research directions and potential therapeutic targets. Our results indicate that hnRNPA1 can affect the metastasis of lung cancer cells by modulating the AS of LAS1L exon 9, highlighting the potential significance of hnRNPA1 in lung cancer metastasis.
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http://dx.doi.org/10.3389/fonc.2022.837248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260696PMC
June 2022

The Integrated Sustainable Development Mechanism of Sports Industry Based on Recombination Fusion Mode.

Comput Intell Neurosci 2022 28;2022:6906467. Epub 2022 Jun 28.

Institute of Physical Education, Sichuan University of Science & Engineering, Zigong 643000, Sichuan, China.

Regular competitions on a bigger size and level are referred to as sports events. The World Cup, the Olympics, Formula, the NBA, and many intercontinental sports events and World Championships of various individual sporting organizations, among others, are among the world's largest and most significant sporting events. In China, there are also specific live sporting event websites that broadcast weekly sporting events. The purpose of this paper is to study the integrated development mechanism for the sustainable development of natural ecotourism and sports events. For this reason, this paper proposes research and analysis on its industrial integration method and industrial integration path, and it has an in-depth understanding of the sustainable development model. At the same time, relevant experiments are designed in the experimental part to explore the impact of sports events and natural ecotourism in the development process. The experimental results of this paper show that after the improvement, the attractiveness of tourists has increased by 47.48%, which has effectively promoted the development of the related tourism industry.
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http://dx.doi.org/10.1155/2022/6906467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256367PMC
July 2022

Carbon Nanotube-Coupled Seaweed-like Cobalt Sulfide as a Dual-Functional Catalyst for Overall Water Splitting.

ACS Appl Mater Interfaces 2022 Jul 27;14(27):30847-30856. Epub 2022 Jun 27.

Key Laboratory of Material Physics, Ministry of Education, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, PR China.

Preparation of high-efficiency dual-functional catalysts remains the bottleneck for electrochemical water splitting. To prepare a non-precious metal catalyst with high activity and stability, here, we present a seaweed-like structure consisting of transition-metal sulfide nanoplates self-assembled on carbon nanotube sponge networks ([email protected]). By adjusting the key parameters during synthesis (e.g., the loading amount and ratio of Co and S precursors), the microstructure can be tailored in a wide range, and sulfur defects can be introduced into the nanoplates by thermal annealing. The resulting [email protected] serves as a freestanding dual-functional catalytic electrode, showing low overpotentials of 105 and 218 mV for the hydrogen evolution reaction and the oxygen evolution reaction, respectively, which are superior to most reported transition-metal-sulfide-based catalysts in alkaline solution. Rational design of this hierarchical biomimetic structure may be useful in developing high-performance electrochemical catalysts in renewable energy and environmental fields.
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http://dx.doi.org/10.1021/acsami.2c06122DOI Listing
July 2022

Reducing Taperin Expression Restores Hearing in Grxcr2 Mutant Mice.

Neuroscience 2022 Jun 23;498:85-92. Epub 2022 Jun 23.

Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address:

Recessive mutations in GRXCR2 cause deafness in both humans and mice. In Grxcr2 null hair cells, the sensory receptors for sound in the inner ear, stereocilia are disorganized. Reducing the expression of taperin, a protein that interacts with GRXCR2 at the base of stereocilia, corrects the morphological defects of stereocilia and restores hearing in Grxcr2 null mice. To further validate this finding, this study generated two novel taperin mutant mouse lines that exhibit progressive hearing loss. Then Grxcr2 null mice were crossed with one of these taperin mutant mice. The following morphological analysis revealed that reducing taperin expression indeed corrected stereocilia morphological abnormalities in Grxcr2 null mice. Functional analysis further confirmed that reducing taperin expression partially restored hearing in Grxcr2 null mice.
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http://dx.doi.org/10.1016/j.neuroscience.2022.06.030DOI Listing
June 2022

Clonidine ameliorates cerebral ischemia-reperfusion injury by up-regulating the GluN3 subunits of NMDA receptor.

Metab Brain Dis 2022 08 21;37(6):1829-1841. Epub 2022 Jun 21.

Third-Grade Pharmacological Laboratory On Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University, Yichang, 443002, People's Republic of China.

This study aimed to investigate the protective effects of the alpha-2 adrenergic receptor (α2-AR) agonist, clonidine, on the cerebral ischemia-reperfusion (I/R) injury and elaborate the underlying mechanisms. Cerebral I/R model was established by middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion for 4 h in adult male SD rats. Saline, clonidine and yohimbine (an α2-AR antagonist) were intraperitoneally administered each day for one week before surgery. Neurological deficit was evaluated just before decapitation. TTC staining was applied for correlation of cerebral infarction volume. HE staining was performed to observe the neuron morphology. Immunohistochemical staining was performed to detect the localization and expression of GluN3 proteins. Western blot analysis also was used to detect the expression levels of GluN3 proteins. Our data showed that clonidine ameliorated neurological deficit and reduced the cerebral infarction volume of the rats with cerebral I/R. It is worth noting that treatment with clonidine up-regulated the protein expression of GluN3 in the rats with the cerebral I/R, especially in the cell membrane. Moreover, clonidine also up-regulated the transposition from cytoplasm to cell membrane of GluN3 after cerebral I/R. In addition, yohimbine abolished the neuroprotective effects of clonidine. The results indicated that clonidine played a protective role in cerebral I/R injury through regulation of the protein expression of GluN3 subunits of N-methyl-D-aspartate (NMDA) receptor.
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http://dx.doi.org/10.1007/s11011-022-01028-yDOI Listing
August 2022

Gender Disparity and the Association Between Socioeconomic Status, Mental Health, and the Need for Long-Term Services and Support Among the Older Koreans.

Front Public Health 2022 3;10:888011. Epub 2022 Jun 3.

Department of Health Administration, Graduate School, Yonsei University, Wonju, South Korea.

Background: Population aging-the inevitable increase in the percentage of older adults-is occurring all around the world as the fertility rate declines and life expectancy rises. This study examined the relationship between socioeconomic status (SES), mental health, and the need for long-term services and support (LTSS) among Korean older adults. It also aimed to provide evidence-based information for South Korea's long-term support services and programs.

Methods: This study used the data on older adults aged over 60 years from the 2018 Korean Longitudinal Study of Aging (KLoSA). Ultimately, 5,527 older adults were included in the database (42.6% men, 57.4% women). To clarify the association between SES, mental health, and the need for LTSS among older Korean men and women, chi-squared test, -test, and structural equation modeling (SEM) were performed.

Results: The SEM analysis showed that a significant, negative association was observed between SES and the need for LTSS among these older adults-the higher SES groups would generally have less need for LTSS. Mental health had a strong, negative impact on the need for LTSS-better mental health status of the older individuals would indicate a lower need for LTSS, and the effect was stronger among older men. Meanwhile, the positive relationship between SES and mental health was verified-the higher the SES of these older adults the better their mental health.

Conclusions: Gender- and social class-sensitive impacts of mental health on the need for LTSS were observed in this study. These findings could provide an evidence-based reference for interventions targeting different genders and social classes in Korea's long-term care system, such as the enhancement of social welfare and mental health status of the older adults.
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http://dx.doi.org/10.3389/fpubh.2022.888011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203953PMC
June 2022

A spin-crossover framework endowed with pore-adjustable behavior by slow structural dynamics.

Nat Commun 2022 Jun 18;13(1):3510. Epub 2022 Jun 18.

Key Laboratory of Cluster Science of Ministry of Education, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 102488, People's Republic of China.

Host-guest interactions play critical roles in achieving switchable structures and functionalities in porous materials, but design and control remain challenging. Here, we report a two-dimensional porous magnetic compound, [Fe(prentrz)Pd(CN)] (prentrz = (1E,2E)-3-phenyl-N-(4H-1,2,4-triazol-4-yl)prop-2-en-1-imine), which exhibits an atypical pore transformation that directly entangles with a spin state transition in response to water adsorption. In this material, the adsorption-induced, non-uniform pedal motion of the axial prentrz ligands and the crumpling/unfolding of the layer structure actuate a reversible narrow quasi-discrete pore (nqp) to large channel-type pore (lcp) change that leads to a pore rearrangement associated with simultaneous pore opening and closing. The unusual pore transformation results in programmable adsorption in which the lcp structure type must be achieved first by the long-time exposure of the nqp structure type in a steam-saturated atmosphere to accomplish the gate-opening adsorption. The structural transformation is accompanied by a variation in the spin-crossover (SCO) property of Fe, i.e., two-step SCO with a large plateau for the lcp phase and two-step SCO with no plateau for the nqp phase. The unusual adsorption-induced pore rearrangement and the related SCO property offer a way to design and control the pore structure and physical properties of dynamic frameworks.
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http://dx.doi.org/10.1038/s41467-022-31274-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206640PMC
June 2022

Tourmaline enhanced methane yield via regulating microbial metabolic balance during anaerobic co-digestion of corn stover and cow manure.

Bioresour Technol 2022 Sep 14;359:127470. Epub 2022 Jun 14.

School of Automation Engineering, Northeast Electric Power University, Jilin 132012, China.

This work performed a pilot-scale study on the effects of the substrate ratio, the concentration of tourmaline (Tur), and its high-temperature thermally modified (HTM) material on the anaerobic co-digestion of corn stover (CS) and cow manure (CM). The experimental results showed that the CH yield was higher at a corn stover -to- cow manure feeding ratio of 2:1. The cumulative CH yield increased by 22.76% and 8.31% at a concentration of tourmaline of 2.0 g/L and a tourmaline treatment temperature of 400 °C respectively. Microbial diversity analysis revealed that adding low doses of tourmaline regulated the distribution of microorganisms and that Methanobacteria became the dominant methanogenic archaea in the fermentation broth. This work clarified the effect of the concentration of tourmaline on gas production by anaerobic co-digestion from the perspective of the microbial metabolic balance and suggested the possibility of its application on a larger scale.
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http://dx.doi.org/10.1016/j.biortech.2022.127470DOI Listing
September 2022

Adaptive Dynamic Programming-Based Event-Triggered Robust Control for Multiplayer Nonzero-Sum Games With Unknown Dynamics1-4mmPlease verify and confirm the term "Multi-Player" has been changed to "Multiplayer" in the title of this article.

IEEE Trans Cybern 2022 Jun 10;PP. Epub 2022 Jun 10.

In this article, the event-triggered robust control of unknown multiplayer nonlinear systems with constrained inputs and uncertainties is investigated by using adaptive dynamic programming. To relax the requirement of system dynamics, a neural network-based identifier is constructed by using the system input-output data. Subsequently, by designing a nonquadratic value function, which contains the bounded functions, the system states, and the control inputs of all players, the event-triggered robust stabilization problem is converted into an event-triggered constrained optimal control problem. To obtain the approximate solution of the event-triggered Hamilton-Jacobi (HJ) equation, a critic network for each player is established with a novel weight updating law to relax the persistence of excitation condition based on the experience replay technique. Furthermore, according to the Lyapunov stability theorem, the present event-triggered robust optimal control ensures the multiplayer system to be uniformly ultimately bounded. Finally, two simulation examples are employed to show the effectiveness of the present method.
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http://dx.doi.org/10.1109/TCYB.2022.3175650DOI Listing
June 2022

Umbilical Cord Mesenchymal Stem Cells Ameliorate Premature Ovarian Insufficiency in Rats.

Evid Based Complement Alternat Med 2022 30;2022:9228456. Epub 2022 May 30.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Premature ovarian insufficiency (POI) or premature ovarian failure (POF) is known as a state of hypergonadotropic hypogonadism. Stem cell therapy is expected to be used in the treatment of POI. The aim of the present study was to explore the feasibility and effectiveness of umbilical cord mesenchymal stem cell (UCMSC) transplantation for the treatment of POI in a rat model of POI induced by cyclophosphamide (CTX) injection. The ovarian function was examined by evaluating the weight of the ovary and body, estrus cycle, ovarian morphology, hormonal secretion, granulosa cell apoptosis, and fertility. The results showed that the ovarian function indicators of the modeled rats were comparable to those of the control rats after UCMSC transplantation, indicating that the ovarian function of the modeled rats recovered to a satisfactory extent. Our research may provide an experimental clue for the clinical application of UCMSC transplantation in the treatment of POI. Further experiments will focus on the detailed signaling pathway study of the molecular mechanisms of injury and repairment on the treatment with UCMSCs transplantation in the rat POI models.
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http://dx.doi.org/10.1155/2022/9228456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170415PMC
May 2022

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Front Cell Dev Biol 2022 20;10:833396. Epub 2022 May 20.

Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

E4B belongs to the U-box E3 ligase family and functions as either an E3 or an E4 enzyme in protein ubiquitination. Transformer2A (TRA2A) and Pyrroline-5-carboxylate reductase 2 (PYCR2) are related to cancer development and are overexpressed in many cancer cells. The degradation of TRA2A and PYCR2 mediated by the ubiquitin-proteasome system (UPS) has not been reported. This study validated that E4B could ubiquitinate TRA2A and PYCR2 as an E3 ligase both and in the HEK293 cells. E4B mediated the degradation by forming K11- and K48- linked polyubiquitin chains on TRA2A and PYCR2, respectively. E4B regulated the alternative splicing function of TRA2A and affected RSRC2 transcription in the HEK293 cells. Although E4B is highly expressed, it hardly degrades TRA2A and PYCR2 in hepatocellular carcinoma (HCC) cells, suggesting other mechanisms exist for degradation of TRA2A and PYCR2 in the HCC cells. We finally reported that E4B interacted with substrates its variable region.
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http://dx.doi.org/10.3389/fcell.2022.833396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163560PMC
May 2022

Molecular-engineered highly photosensitive triarylphosphine oxide compounds for apoptosis imaging and selectively inducing apoptosis of tumor cells by photodynamic therapy.

Biomater Sci 2022 Jun 28;10(13):3441-3446. Epub 2022 Jun 28.

School of Pharmacy, Sichuan Key Laboratory of Medical Imaging, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Sichuan 637100, China.

Although photodynamic therapy (PDT) has wide applications, tumor-targeting probes with high photosensitivity or apoptosis-monitoring capability, which possess low phototoxicity and can be used for evaluating therapeutic efficacy, are still scarce. In this study, we constructed a series of highly photosensitive probes by introducing multivalent positive charges around propeller-like triarylphosphine oxide compounds. Some of them can be used to detect apoptosis by selectively entering apoptotic cells in the presence of living or necrotic cells. Among them, OTPP-6-Amyl can target SKOV-3 cells by binding to their membrane in a short time and move to mitochondria with prolonging time and can be further applied for imaging SKOV-3 tumors , whereas for various apoptotic cells, it mainly enters the nucleus. Its high photosensitivity can be used to induce apoptosis in SKOV-3 cells without affecting the survival of other cells. By functionalizing with cRGD, another probe was constructed to target U87MG cells and induce their apoptosis by PDT, and it can also pass through the blood-brain barrier.
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http://dx.doi.org/10.1039/d2bm00462cDOI Listing
June 2022

Cancer-associated mutations in SF3B1 disrupt the interaction between SF3B1 and DDX42.

J Biochem 2022 Jul;172(2):117-126

China-Japan Union Hospital of Jilin University, Jilin University, Changchun, Jilin 130033, China.

While cancer-associated SF3B1 mutations causes alternative RNA splicing, the molecular mechanism underlying the alternative RNA splicing is not fully elucidated. Here, we analysed the proteins that interacted with the wild-type and K700E-mutated SF3B1 and found that the interactions of two RNA helicases, DDX42 and DDX46, with the mutated SF3B1 were reduced. Overexpression of DDX42 restored the decreased interaction between DDX42 and the K700E-mutated SF3B1, and suppressed some alternative RNA splicing associated with the SF3B1 mutation. Mutation that decreased the ATP hydrolysis activities of DDX42 abolished the suppressive effects of DDX42 on the alternative RNA splicing, suggesting that the ATP hydrolysis activity of DDX42 is involved in the mechanism of the altered RNA splicing associated with the SF3B1 mutation. Our study demonstrates an important function of the interaction between DDX42 and SF3B1 on regulating RNA splicing and revealed a potential role of DDX42 in the altered RNA splicing associated with the SF3B1 mutation.
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http://dx.doi.org/10.1093/jb/mvac049DOI Listing
July 2022

Application of Convolutional Neural Network-Based Detection Methods in Fresh Fruit Production: A Comprehensive Review.

Front Plant Sci 2022 16;13:868745. Epub 2022 May 16.

School of Electronic and Information Engineering, Taizhou University, Taizhou, China.

As one of the representative algorithms of deep learning, a convolutional neural network (CNN) with the advantage of local perception and parameter sharing has been rapidly developed. CNN-based detection technology has been widely used in computer vision, natural language processing, and other fields. Fresh fruit production is an important socioeconomic activity, where CNN-based deep learning detection technology has been successfully applied to its important links. To the best of our knowledge, this review is the first on the whole production process of fresh fruit. We first introduced the network architecture and implementation principle of CNN and described the training process of a CNN-based deep learning model in detail. A large number of articles were investigated, which have made breakthroughs in response to challenges using CNN-based deep learning detection technology in important links of fresh fruit production including fruit flower detection, fruit detection, fruit harvesting, and fruit grading. Object detection based on CNN deep learning was elaborated from data acquisition to model training, and different detection methods based on CNN deep learning were compared in each link of the fresh fruit production. The investigation results of this review show that improved CNN deep learning models can give full play to detection potential by combining with the characteristics of each link of fruit production. The investigation results also imply that CNN-based detection may penetrate the challenges created by environmental issues, new area exploration, and multiple task execution of fresh fruit production in the future.
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http://dx.doi.org/10.3389/fpls.2022.868745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149381PMC
May 2022

GAN-Based Video Denoising with Attention Mechanism for Field-Applicable Pig Detection System.

Sensors (Basel) 2022 May 22;22(10). Epub 2022 May 22.

Department of Computer Convergence Software, Korea University, Sejong Campus, Sejong City 30019, Korea.

Infrared cameras allow non-invasive and 24 h continuous monitoring. Thus, they are widely used in automatic pig monitoring, which is essential to maintain the profitability and sustainability of intensive pig farms. However, in practice, impurities such as insect secretions continuously pollute camera lenses. This causes problems with IR reflections, which can seriously affect pig detection performance. In this study, we propose a noise-robust, real-time pig detection system that can improve accuracy in pig farms where infrared cameras suffer from the IR reflection problem. The system consists of a data collector to collect infrared images, a preprocessor to transform noisy images into clean images, and a detector to detect pigs. The preprocessor embeds a multi-scale spatial attention module in U-net and generative adversarial network (GAN) models, enabling the model to pay more attention to the noisy area. The GAN model was trained on paired sets of clean data and data with simulated noise. It can operate in a real-time and end-to-end manner. Experimental results show that the proposed preprocessor was able to significantly improve the average precision of pig detection from 0.766 to 0.906, with an additional execution time of only 4.8 ms on a PC environment.
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http://dx.doi.org/10.3390/s22103917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9143193PMC
May 2022

Magnetic Properties and Washability of Roasted Suspended Siderite Ores.

Materials (Basel) 2022 May 17;15(10). Epub 2022 May 17.

College of Materials Science and Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

Steel is one of the most important industrial materials, which mainly comes from the smelting of iron ore. In view of the huge steel consumption every year, the exploitation of vast reserves of siderite ores is significant for improving the self-sufficiency rate of iron ore resources and ensuring the strategic security of the iron and steel industries. This paper investigated the influence of temperature, time, and other parameters on the magnetic properties of roasted siderite ores using the method of suspended roasting and analyzed the washability of roasted ores under weak-magnetic-field conditions using the magnetic separation tube experiment. The findings of the study explained the iron phase transformation process, i.e., FeCO was transformed into FeO by suspension magnetization roasting. Furthermore, the saturation magnetization of the roasted ore increased in due time at a constant temperature range of 550-750 °C and a roasting time of less than 5 s. It also increased with increasing temperature and constant time. The roasted ore achieved the best magnetic characteristics after roasting at 750 °C for 5 s. After low-intensity magnetic separation, the iron grade of the concentrate changed to 55.12%, with a recovery rate of 90.34%. The study results provide a reference for the development and application of siderite suspension magnetization roasting technology.
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http://dx.doi.org/10.3390/ma15103582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9142997PMC
May 2022

Exploring the spatial disparity of home-dwelling time patterns in the USA during the COVID-19 pandemic via Bayesian inference.

Trans GIS 2022 Jun 17;26(4):1939-1961. Epub 2022 Mar 17.

Department of Geography University of South Carolina Columbia South Carolina USA.

In this study, we aim to reveal hidden patterns and confounders associated with policy implementation and adherence by investigating the home-dwelling stages from a data-driven perspective via Bayesian inference with weakly informative priors and by examining how home-dwelling stages in the USA varied geographically, using fine-grained, spatial-explicit home-dwelling time records from a multi-scale perspective. At the U.S. national level, two changepoints are identified, with the former corresponding to March 22, 2020 (9 days after the White House declared the National Emergency on March 13) and the latter corresponding to May 17, 2020. Inspections at U.S. state and county level reveal notable spatial disparity in home-dwelling stage-related variables. A pilot study in the Atlanta Metropolitan area at the Census Tract level reveals that the self-quarantine duration and increase in home-dwelling time are strongly correlated with the median household income, echoing existing efforts that document the economic inequity exposed by the U.S. stay-at-home orders. To our best knowledge, our work marks a pioneering effort to explore multi-scale home-dwelling patterns in the USA from a purely data-driven perspective and in a statistically robust manner.
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http://dx.doi.org/10.1111/tgis.12918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115371PMC
June 2022
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