Publications by authors named "Bo Zhao"

938 Publications

Normalizing Clinical Document Titles to LOINC Document Ontology: an Initial Study.

AMIA Annu Symp Proc 2020 25;2020:1441-1450. Epub 2021 Jan 25.

School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, USA.

The normalization of clinical documents is essential for health information management with the enormous amount of clinical documentation generated each year. The LOINC Document Ontology (DO) is a universal clinical document standard in a hierarchical structure. The objective of this study is to investigate the feasibility and generalizability of LOINC DO by mapping from clinical note titles across five institutions to five DO axes. We first developed an annotation framework based on the definition of LOINC DO axes and manually mapped 4,000 titles. Then we introduced a pre-trained deep learning model named Bidirectional Encoder Representations from Transformers (BERT) to enable automatic mapping from titles to LOINC DO axes. The results showed that the BERT-based automatic mapping achieved improved performance compared with the baseline model. By analyzing both manual annotations and predicted results, ambiguities in LOINC DO axes definition were discussed.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075502PMC
January 2021

Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration.

Stem Cell Res Ther 2021 May 1;12(1):257. Epub 2021 May 1.

Research Center for Translational Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200092, China.

Background: Cutaneous wound healing and regeneration have become a recognized health challenge in the world, which causes severe damage to the mental and physical health of patients. Human adipose-derived mesenchymal stem cells (hADSC) play an essential role in wound healing via their paracrine function. Exosomes secreted by hADSC may contribute to this progress. In this study, we investigated the potential clinical application roles of hADSC and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing.

Methods: hADSC-Exo was isolated from human hADSC by ultracentrifugation. Mice were subjected to a full-thickness skin biopsy experiment and treated with either control vehicle or hADSC or hADSC-Exo by smearing administration (sm) or subcutaneous administration (sc) or intravenous administration (iv). The efficacy of hADSC and hADSC-Exo on wound healing was evaluated by measuring wound closure rates, histological analysis.

Results: Combined application of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration offered the additional benefit of promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis. Either topical application of hADSC-Exo or systemic administration with hADSC/hADSC-Exo appeared more effective in stimulating cell proliferation, inhibiting cell apoptosis and inflammation, and promoting skin elasticity and barrier integrity, with increased genes expression of PCNA, VEGF, collagen III, Filaggrin, Loricrin, and AQP3, with decreased genes expression of TNF-alpha.

Conclusion: Our findings suggest that the combined administration of hADSC/hADSC-Exo can facilitate cutaneous wound healing and reduce scar formation. These data provide the first evidence for the feasibility of smearing of hADSC-Exo as a cell-free therapy in treating cutaneous wounds, and the potential clinical value of combined administration of hADSC/hADSC-Exo.
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http://dx.doi.org/10.1186/s13287-021-02287-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088044PMC
May 2021

2,4-DCP compromises the fertilization capacity of mouse oocytes.

J Cell Physiol 2021 Apr 30. Epub 2021 Apr 30.

Department of Aquaculture, School of Fisheries, Zhejiang Ocean University, Zhoushan, Zhejiang, China.

2,4-DCP (2,4-dichlorophenol) is an environmental estrogen that is ubiquitously distributed in the environment and widely used to produce herbicides and pharmaceutical intermediates. Although 2,4-DCP is suspected to have endocrine disruption, the reproductive toxicity of 2,4-DCP in mammals has not been adequately assessed. In the present study, we examined the effect of 2,4-DCP on the fertility of mouse eggs. The data showed that oral administration of 2,4-DCP (180 mg/kg/day for 7 days) compromises the fertilization rate of mouse oocytes. To further analyze the mechanism by which 2,4-DCP decreases fertilization, the key regulators and events during fertilization of mouse eggs were investigated. We found that the dynamics of cortical granules (CGs) were disrupted by showing the redistribution of CG free domain in 2,4-DCP-administered oocytes. This abnormality perturbed the sperm binding site in the zona pellucida (ZP) and dramatically reduced the number of sperm binding to the ZP of 2,4-DCP-administered oocytes. In addition, the abundance of Juno, a sperm receptor on the egg membrane, was also decreased and its distribution was mislocated in 2,4-DCP-administered oocytes. Finally, we validated that the defects of fertilization participants and events caused by 2,4-DCP might be mediated by the increased level of reactive oxygen species-induced apoptosis of oocytes. Therefore, we demonstrate that 2,4-DCP compromises the fertilization ability of mouse oocytes via inducing oxidative stress.
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http://dx.doi.org/10.1002/jcp.30403DOI Listing
April 2021

Color-Tunable White LEDs with Single Chip Realized through Phosphor Pattern and Thermal-Modulating Optical Film.

Micromachines (Basel) 2021 Apr 12;12(4). Epub 2021 Apr 12.

School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749, Korea.

In this paper, a new method to regulate the correlated color temperature (CCT) of white light-emitting diodes (LEDs) is proposed for the single-chip packaging structure, in which the blue light distribution emitted from the chip in the red/yellow phosphor layer was modulated through changing the paraffin-polydimethylsiloxane (PDMS) film transparence and haze. The results show that the transmittance of the paraffin-PDMS film can be modulated from 49.76% to 97.64%, while the haze of that ranges from 88.19% to 63.10%. When the thickness of paraffin-PDMS film is 0.6 mm, and the paraffin-PDMS film concentration is 30 wt%, the CCT of white LED decreases from 15177 K to 3615 K with the increase of thermal load in the paraffin-PDMS film. The modulating range of its CCT reaches 11562 K. The maximum CCT variation at the same test condition is only 536 K in the repeated experiments within one week.
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http://dx.doi.org/10.3390/mi12040421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069560PMC
April 2021

Assessing Knowledge, Preventive Practices, and Depression among Chinese University Students in Korea and China during the COVID-19 Pandemic: An Online Cross-Sectional Study.

Healthcare (Basel) 2021 Apr 8;9(4). Epub 2021 Apr 8.

Department of Health Administration, Graduate School, Yonsei University, 1 Yonseidae-gil, Wonju 26493, Korea.

To investigate the knowledge, preventive practices, and depression of Chinese university students living in South Korea and Mainland China during the COVID-19 outbreak and explore the determinants of depression among these students, an online cross-sectional questionnaire survey was conducted from 23 March to 12 April 2020. The online questionnaire included questions on knowledge and preventive practices related to COVID-19, and the Patient Health Questionnaire-9 was used to diagnose depressive symptoms. A total of 420 Chinese university students were finally included in the study (171 students from South Korea and 249 students from Mainland China). The majority of these students had a good level of knowledge of COVID-19. Students living in South Korea displayed better preventive practices than those living in Mainland China; however, the proportion of students (28.7%) with moderate-to-severe depression in this group was relatively higher than that (18.9%) of the Mainland Group (χ = 5.50, < 0.05). More severe depression was related to high levels of concern about family members and contracting COVID-19 as well as suspecting themselves of having come into contact with patients. Displaying more preventive behaviors decreased the depressive symptoms in both groups. These data could be used as a reference for further studies in different regions to take measures (e.g., psychological counseling and encouragement for physical activities) to reduce depressive symptoms in university students.
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http://dx.doi.org/10.3390/healthcare9040433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067962PMC
April 2021

High-speed femtosecond laser plasmonic lithography and reduction of graphene oxide for anisotropic photoresponse.

Light Sci Appl 2020 Apr 26;9(1):69. Epub 2020 Apr 26.

State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 130033, Changchun, China.

Micro/nanoprocessing of graphene surfaces has attracted significant interest for both science and applications due to its effective modulation of material properties, which, however, is usually restricted by the disadvantages of the current fabrication methods. Here, by exploiting cylindrical focusing of a femtosecond laser on graphene oxide (GO) films, we successfully produce uniform subwavelength grating structures at high speed along with a simultaneous in situ photoreduction process. Strikingly, the well-defined structures feature orientations parallel to the laser polarization and significant robustness against distinct perturbations. The proposed model and simulations reveal that the structure formation is based on the transverse electric (TE) surface plasmons triggered by the gradient reduction of the GO film from its surface to the interior, which eventually results in interference intensity fringes and spatially periodic interactions. Further experiments prove that such a regular structured surface can cause enhanced optical absorption (>20%) and an anisotropic photoresponse (~0.46 ratio) for the reduced GO film. Our work not only provides new insights into understanding the laser-GO interaction but also lays a solid foundation for practical usage of femtosecond laser plasmonic lithography, with the prospect of expansion to other two-dimensional materials for novel device applications.
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http://dx.doi.org/10.1038/s41377-020-0311-2DOI Listing
April 2020

Regression analysis of current status data with latent variables.

Lifetime Data Anal 2021 Apr 24. Epub 2021 Apr 24.

Department of Statistics, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

Current status data occur in many fields including demographical, epidemiological, financial, medical, and sociological studies. We consider the regression analysis of current status data with latent variables. The proposed model consists of a factor analytic model for characterizing latent variables through their multiple surrogates and an additive hazard model for examining potential covariate effects on the hazards of interest in the presence of current status data. We develop a borrow-strength estimation procedure that incorporates the expectation-maximization algorithm and correlated estimating equations. The consistency and asymptotic normality of the proposed estimators are established. A simulation study is conducted to evaluate the finite sample performance of the proposed method. A real-life study on the chronic kidney disease of type 2 diabetic patients is presented.
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http://dx.doi.org/10.1007/s10985-021-09521-9DOI Listing
April 2021

Transcriptome analysis of signaling pathways targeted by Ellagic acid in hepatocellular carcinoma cells.

Biochim Biophys Acta Gen Subj 2021 Apr 20;1865(7):129911. Epub 2021 Apr 20.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin 150040, China. Electronic address:

Background: Ellagic acid (EA) possesses prominent inhibitory activities against various cancers, including hepatocellular carcinoma (HCC). Our recent study demonstrated EA's activities in reducing HCC cell proliferation and tumor formation. However, the mechanisms of EA to exert its anticancer activities and its primary targets in cancer cells have not been systematically explored.

Methods: Cell proliferation assay and flow cytometric analysis were used to examine the effects of EA treatment on viability and apoptosis, respectively, of HepG2 cells. RNA-seq studies and associated pathway analyses by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to determine EA's primary targets. Differentially expressed genes (DEG) in EA-treated HepG2 cells were verified by RT-qPCR and Western blot. Integrative analyses of the RNA-seq dataset with a TCGA dataset derived from HCC patients were conducted to verify EA-targeted genes and signaling pathways. Interaction network analysis of the DEGs, shRNA-mediated knockdown, cell viability assay, and colony formation assay were used to validate EA's primary targets.

Results: EA reduced cell viability, caused DNA damage, and induced cell cycle arrest at G1 phase of HepG2 cells. We identified 5765 DEGs encoding proteins with over 2.0-fold changes in EA-treated HepG2 cells by DESeq2. These DEGs showed significant enrichment in the pathways regulating DNA replication and cell cycle progression. As primary targets, p21 was significantly upregulated, while MCM2-7 were uniformly downregulated in response to EA treatment. Consistently, p21 knockdown desensitized liver cells to EA in cell viability and colony formation assays.

Conclusion: EA induced G1 phase arrest and promoted apoptosis of HCC cells through activating the p21 gene and downregulating the MCM2-7 genes, respectively.

General Significance: The discoveries in this study provide helpful insights into developing novel strategies in the therapeutic treatment of HCC patients.
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http://dx.doi.org/10.1016/j.bbagen.2021.129911DOI Listing
April 2021

Estimating the impact of ground ozone concentrations on crop yields across China from 2014 to 2018: A multi-model comparison.

Environ Pollut 2021 Apr 7;283:117099. Epub 2021 Apr 7.

State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Global and Earth System Sciences, Beijing Normal University, 19 Xinjiekou Street, Haidian, Beijing, 100875, China. Electronic address:

Ground level ozone exerts a strong impact on crop yields, yet how to properly quantify ozone induced yield losses in China remains challenging. To this end, we employed a series of O-crop models to estimate ozone induced yield losses in China from 2014 to 2018. The outputs from all models suggested that the total Relative Yield Losses (RYL) of wheat in China from 2014 to 2018 was 18.4%-49.3% and the total RYL of rice was 6.2%-52.9%. Consequently, the total Crop Production Losses (CPL) of wheat and rice could reach 63.9-130.4 and 28.3-35.4 million tons, and the corresponding Total Economic Losses (TEL) could reach 20.5-44.7 and 11.0-15.3 billion dollars, stressing the great importance and urgency of national ozone management. Meanwhile, the estimation outputs highlighted the large variations between different regional O-crop models when applying to large scales. Instead of applying one unified O-crop models to all regions across China, we also explored the strategy of employing specific O-crop models in corresponding (and neighboring) regions to estimate ozone induced yield loss in China. The comparison of two strategies suggested that the mean value from multiple models may still present an inconsistent over/underestimation trend for different crops. Therefore, it is a preferable strategy to employ corresponding O-crop models in different regions for estimating the national crop losses caused by ozone pollution. However, the severe lack of regional O-crop models in most regions across China makes a robust estimation of national yield losses highly challenging. Given the large variations between O-crop interactions across regions, a systematic framework with massive regional O-crop models should be properly designed and implemented.
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http://dx.doi.org/10.1016/j.envpol.2021.117099DOI Listing
April 2021

Discovery of novel pyrimidine molecules containing boronic acid as VCP/p97 Inhibitors.

Bioorg Med Chem 2021 May 23;38:116114. Epub 2021 Mar 23.

College of Life Science, Nanjing Normal University, No. 1 Wenyuan Road, Nanjing 210037, PR China. Electronic address:

Valine-containing protein (VCP) is a member of the adenosine triphosphate family involved in a variety of cellular activities. VCP/p97 is capable of maintaining protein homeostasis and mediating the degradation of misfolded polypeptides by the ubiquitin-proteasome system (UPS). In this manuscript, a series of novel p97 inhibitors with pyrimidine as core structure were designed, synthesized and biologically evaluated. Based on the enzymatic results, a detailed structure-activity relationship discussion of the synthesized compounds was carried out. Furthermore, cellular activities of the compounds with enzymatic potency of less than 200 nM were investigated by using A549 and RPMI8226 cell lines. Among the screened inhibitors, compound 17 (IC, 54.7 nM) showed good enzymatic activity. Investigation of cellular activities with non-small cell lung cancer A549 and multiple myeloma (MM) RPMI8226 further confirmed the potency of 17 with the IC values of 2.80 μM and 0.86 μM, respectively. Compound 17 is now being developed as a candidate. Finally, docking studies were carried out to explore the possible binding mode between the active inhibitor 17 and p97.
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http://dx.doi.org/10.1016/j.bmc.2021.116114DOI Listing
May 2021

Variation in Initial U.S. Hospital Responses to the Coronavirus Disease 2019 Pandemic.

Crit Care Med 2021 Apr 8. Epub 2021 Apr 8.

Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY. Department of Emergency Medicine, Icahn School of Medicine at Mount Sinai, New York, NY. Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Vanderbilt University, Nashville, TN. Health Services Research & Development, VA Portland Health Care System, Portland, OR. Division of Pulmonary and Critical Care, Department of Medicine, Oregon Health & Science University, Portland, OR. Palliative and Advanced Illness Research (PAIR) Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. Department of Critical Care, Respiratory Institute, Cleveland Clinic, Cleveland, OH. Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI. Institute for Healthcare Policy and Innovation, University of Michigan, Ann Arbor, MI. Center for Bioethics and Social Sciences in Medicine, University of Michigan, Ann Arbor, MI. Department of Geography, University of Washington, Seattle, WA. Department of Emergency Medicine, University of Washington, Seattle, WA. Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. Division of Pulmonary and Critical Care Medicine, Department of Medicine, David Geffen School of Medicine at UCLA, Ronald Reagan-UCLA Medical Center, Los Angeles, CA.

Objectives: The coronavirus disease 2019 pandemic has strained many healthcare systems. In response, U.S. hospitals altered their care delivery systems, but there are few data regarding specific structural changes. Understanding these changes is important to guide interpretation of outcomes and inform pandemic preparedness. We sought to characterize emergency responses across hospitals in the United States over time and in the context of local case rates early in the coronavirus disease 2019 pandemic.

Design: We surveyed hospitals from a national acute care trials group regarding operational and structural changes made in response to the coronavirus disease 2019 pandemic from January to August 2020. We collected prepandemic characteristics and changes to hospital system, space, staffing, and equipment during the pandemic. We compared the timing of these changes with county-level coronavirus disease 2019 case rates.

Setting And Participants: U.S. hospitals participating in the Prevention and Early Treatment of Acute Lung Injury Network Coronavirus Disease 2019 Observational study. Site investigators at each hospital collected local data.

Interventions: None.

Measurements And Main Results: Forty-five sites participated (94% response rate). System-level changes (incident command activation and elective procedure cancellation) occurred at nearly all sites, preceding rises in local case rates. The peak inpatient census during the pandemic was greater than the prior hospital bed capacity in 57% of sites with notable regional variation. Nearly half (49%) expanded ward capacity, and 63% expanded ICU capacity, with nearly all bed expansion achieved through repurposing of clinical spaces. Two-thirds of sites adapted staffing to care for patients with coronavirus disease 2019, with 48% implementing tiered staffing models, 49% adding temporary physicians, nurses, or respiratory therapists, and 30% changing the ratios of physicians or nurses to patients.

Conclusions: The coronavirus disease 2019 pandemic prompted widespread system-level changes, but front-line clinical care varied widely according to specific hospital needs and infrastructure. Linking operational changes to care delivery processes is a necessary step to understand the impact of the coronavirus disease 2019 pandemic on patient outcomes.
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http://dx.doi.org/10.1097/CCM.0000000000005013DOI Listing
April 2021

Dosimetric feasibility of stereotactic ablative radiotherapy in pulmonary vein isolation for atrial fibrillation using intensity-modulated proton therapy.

J Appl Clin Med Phys 2021 Apr 4. Epub 2021 Apr 4.

Department of Radiation Oncology, Mayo Clinic Arizona, Phoenix, AZ, USA.

Purpose: To evaluate dosimetric properties of intensity-modulated proton therapy (IMPT) for simulated treatment planning in patients with atrial fibrillation (AF) targeting left atrial-pulmonary vein junction (LA-PVJ), in comparison with volumetric-modulated arc therapy (VMAT) and helical tomotherapy (TOMO).

Methods: Ten thoracic 4D-CT scans with respiratory motion and one with cardiac motion were used for the study. Ten respiratory 4D-CTs were planned with VMAT, TOMO, and IMPT for simulated AF. Targets at the LA-PVJ were defined as wide-area circumferential ablation line. A single fraction of 25 Gy was prescribed to all plans. The interplay effects from cardiac motion were evaluated based on the cardiac 4D-CT scan. Dose-volume histograms (DVHs) of the ITV and normal tissues were compared. Statistical analysis was evaluated via one-way Repeated-Measures ANOVA and Friedman's test with Bonferroni's multiple comparisons test.

Results: The median volume of ITV was 8.72cc. All plans had adequate target coverage (V  ≥ 99%). Compared with VMAT and TOMO, IMPT resulted in significantly lower dose of most normal tissues. For VMAT, TOMO, and IMPT plans, D of the whole heart was 5.52 ± 0.90 Gy, 5.89 ± 0.78 Gy, and 3.01 ± 0.57 Gy (P < 0.001), mean dose of pericardium was 4.74 ± 0.76 Gy, 4.98 ± 0.62 Gy, and 2.59 ± 0.44 Gy (P < 0.001), and D of left circumflex artery (LCX) was 13.96 ± 5.45 Gy, 14.34 ± 5.91 Gy, and 8.43 ± 7.24 Gy (P < 0.001), respectively. However, no significant advantage for one technique over the others was observed when examining the D of esophagus and main bronchi.

Conclusions: IMPT targeting LA-PVJ for patients with AF has high potential to reduce dose to surrounding tissues compared to VMAT or TOMO. Motion mitigation techniques are critical for a particle-therapy approach.
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http://dx.doi.org/10.1002/acm2.13239DOI Listing
April 2021

Long non-coding RNA NEAT1 mediates MPTP/MPP-induced apoptosis via regulating the miR-124/KLF4 axis in Parkinson's disease.

Open Life Sci 2020 6;15(1):665-676. Epub 2020 Sep 6.

Department of Neurology, Anning Branch of the 940th Hospital of Joint Logistic Support Force of PLA, No. 1026, East-Anning Road, Lanzhou, Gansu, China.

Accumulating evidence suggests that dysregulation of long non-coding RNAs is closely associated with various human diseases, including Parkinson's disease (PD). However, the role of nuclear-enriched abundant transcript 1 (NEAT1) in the PD process remains unclear. The number of TH+ cells was reduced, and the expression levels of NEAT1 and Krüppel-like factor 4 (KLF4) were increased in the midbrain of MPTP-HCl-treated mice. In addition, the expression of cleaved-caspase-3 (cleaved-casp-3) and Bax (apoptosis-related proteins) was increased, while the expression of Bcl-2 (anti-apoptotic protein) was reduced in MPTP-HCl-treated mice. The expression levels of NEAT1 and KLF4 were increased in MPP-treated SH-SY5Y cells. Knockdown of NEAT1 promoted cell viability and decreased apoptosis in MPP-treated SH-SY5Y cells, which could be reversed by upregulating KLF4. KLF4 was verified as a direct target of miR-124, and miR-124 could particularly bind to NEAT1. Downregulation of NEAT1 significantly increased cell viability and decreased apoptosis by regulating miR-124 expression in MPP-treated SH-SY5Y cells. Additionally, interference of NEAT1 increased the number of TH+ cells and miR-124 expression, while reduced apoptosis and expression of KLF4 . NEAT1 knockdown increased cell viability and suppressed apoptosis in PD via regulating the miR-124/KLF4 axis, providing a promising avenue for the treatment of PD.
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http://dx.doi.org/10.1515/biol-2020-0069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747504PMC
September 2020

Toxic effect of three imidazole ionic liquids on two terrestrial plants.

Open Life Sci 2020 6;15(1):466-475. Epub 2020 Jul 6.

Institution Shaanxi Key Laboratory of Phytochemistry, College of Chemistry & Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China.

To determine the toxic effect of three imidazole ionic liquids (IILs) in terrestrial monocotyledonous and dicotyledonous plants, three IILs (1-butyl-3-methylimidazole tetrafluoroborate, 1-butyl-3-methylimidazole hexafluorophosphate, and butyl-3-methylimidazolium bi-[(trifluoromethyl)sulfonyl]imide) were investigated using rice and capsicum as target toxicity models. In hydroponic experiments, increasing the concentration of the IILs led to a decrease in the seed germination rate, a decrease in the reduced stem and root lengths, and an increase in the inhibition rate of the stem and root lengths; in addition, as the concentration increased, the reducing sugar content of rice and capsicum seedling leaves and roots first increased and then decreased, while permeability of the cell membranes of the stems and roots of the two plants also gradually increased. In terms of the effects on these indices in rice, the ranking of these three IIL anions was [TF2N]- > [PF6]- > [BF4]-; in terms of the effects on capsicum, the sequence was [BF4]- > [TF2N]- > [PF6]-. These findings provide a theoretical reference for the next step in the synthesis and the use of green ionic liquids.
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http://dx.doi.org/10.1515/biol-2020-0051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874614PMC
July 2020

Design and Experimentation of a Self-Sensing Actuator for Active Vibration Isolation System with Adjustable Anti-Resonance Frequency Controller.

Sensors (Basel) 2021 Mar 10;21(6). Epub 2021 Mar 10.

Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China.

The vibration isolation system is now indispensable to high-precision instruments and equipment, which can provide a low vibration environment to ensure performance. However, the disturbance with variable frequency poses a challenge to the vibration isolation system, resulting in precision reduction of dynamic modeling. This paper presents a velocity self-sensing method and experimental verification of a vibration isolation system. A self-sensing actuator is designed to isolate the vibration with varying frequencies according to the dynamic vibration absorber structure. The mechanical structure of the actuator is illustrated, and the dynamic model is derived. Then a self-sensing method is proposed to adjust the anti-resonance frequency of the system without velocity sensors, which can also reduce the complexity of the system and prevent the disturbance transmitting along the cables. The self-sensing controller is constructed to track the variable frequency of the disturbance. A prototype of the isolation system equipped with velocity sensors is developed for the experiment. The experiment results show that the closed-loop transmissibility is less than -5 dB in the whole frequency rand and is less than -40 dB around, adding anti-resonance frequency which can be adjusted from 0 Hz to initial anti-resonance frequency. The disturbance amplitude of the payload can be suppressed to 10%.
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http://dx.doi.org/10.3390/s21061941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001811PMC
March 2021

Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread.

Cells 2021 03 28;10(4). Epub 2021 Mar 28.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.

Parkinson's disease (PD) is the most common neurodegenerative movement disorder, characterized by progressive loss of dopaminergic neurons in the substantia nigra, intraneuronal deposition of misfolded proteins known as Lewy bodies, and chronic neuroinflammation. PD can arise from monogenic mutations, but in most cases, the etiology is unclear. Viral infection is gaining increasing attentions as a trigger of PD. In this study, we investigated whether the PD-causative 620 aspartate (D) to asparagine (N) mutation in the vacuolar protein sorting 35 ortholog (Vps35) precipitated herpes simplex virus (HSV) infection. We observed that ectopic expression of Vps35 significantly reduced the proliferation and release of HSV-1 virions; the D620N mutation rendered Vps35 a partial loss of such inhibitory effects. Tetherin is a host cell protein capable of restricting the spread of encapsulated viruses including HSV-1 and SARS-Cov-2, both of which are implicated in the development of parkinsonism. Compared with cells overexpressing wildtype Vps35, cells expressing mutant Vps35 with D620N had less Tetherin on cell surfaces. Real-time and static cell imaging revealed that Tetherin recycled through Vps35-positive endosomes. Expression of Vps35 with D620N reduced endosomal dynamics and frequency of motile Tetherin-containing vesicles, a sign of defective production of recycling carriers. Our study suggests that the D620N mutation in Vps35 hinders Tetherin trafficking to cell surfaces and facilitates virus spread.
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http://dx.doi.org/10.3390/cells10040746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066283PMC
March 2021

EIN2-directed histone acetylation requires EIN3-mediated positive feedback regulation in response to ethylene.

Plant Cell 2021 Apr;33(2):322-337

Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA.

Ethylene is an important phytohormone with pleotropic roles in plant growth, development, and stress responses. ETHYLENE INSENSITIVE2 (EIN2) mediates the transduction of the ethylene signal from the endoplasmic reticulum membrane to the nucleus, where its C-terminus (EIN2-C) regulates histone acetylation to mediate transcriptional regulation by EIN3. However, no direct interaction between EIN2-C and EIN3 has been detected. To determine how EIN2-C and EIN3 act together, we followed a synthetic approach and engineered a chimeric EIN2-C with EIN3 DNA-binding activity but lacking its transactivation activity (EIN2C-EIN3DB). The overexpression of EIN2C-EIN3DB in either wild-type or in the ethylene-insensitive mutant ein3-1 eil1-1 led to a partial constitutive ethylene response. Chromatin immunoprecipitation sequencing showed that EIN2C-EIN3DB has DNA-binding activity, indicating that EIN3DB is functional in EIN2C-EIN3DB. Furthermore, native EIN3 protein levels determine EIN2C-EIN3DB binding activity and binding targets in a positive feedback loop by interacting with EIN2C-EIN3DB to form a heterodimer. Additionally, although EIN3 does not direct affect histone acetylation levels in the absence of EIN2, it is required for the ethylene-induced elevation of H3K14Ac and H3K23Ac in the presence of EIN2. Together, we reveal efficient and specific DNA-binding by dimerized EIN3 in the presence of ethylene to mediate positive feedback regulation, which is required for EIN2-directed elevation of histone acetylation to integrate into an EIN3-dependent transcriptional activation.
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http://dx.doi.org/10.1093/plcell/koaa029DOI Listing
April 2021

DEK domain-containing proteins control flowering time in Arabidopsis.

New Phytol 2021 Mar 28. Epub 2021 Mar 28.

Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712, USA.

Evolutionarily conserved DEK domain-containing proteins have been implicated in multiple chromatin-related processes, mRNA splicing and transcriptional regulation in eukaryotes. Here, we show that two DEK proteins, DEK3 and DEK4, control the floral transition in Arabidopsis. DEK3 and DEK4 directly associate with chromatin of related flowering repressors, FLOWERING LOCUS C (FLC), and its two homologs, MADS AFFECTING FLOWERING4 (MAF4) and MAF5, to promote their expression. The binding of DEK3 and DEK4 to a histone octamer in vivo affects histone modifications at FLC, MAF4 and MAF5 loci. In addition, DEK3 and DEK4 interact with RNA polymerase II and promote the association of RNA polymerase II with FLC, MAF4 and MAF5 chromatin to promote their expression. Our results show that DEK3 and DEK4 directly interact with chromatin to facilitate the transcription of key flowering repressors and thus prevent precocious flowering in Arabidopsis.
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http://dx.doi.org/10.1111/nph.17366DOI Listing
March 2021

Characterization of the aberrant splicing of MAP3K7 induced by cancer-associated SF3B1 mutation.

J Biochem 2021 Mar 5. Epub 2021 Mar 5.

China-Japan Union Hospital of Jilin University, Jilin University, Changchun, Jilin, 130033, China.

SF3B1, an essential RNA splicing factor, is frequently mutated in various types of cancers, and the cancer-associated SF3B1 mutation causes aberrant RNA splicing. The aberrant splicing of several transcripts, including MAP3K7, promotes tumorigenesis. Here, we identify a premature termination codon in the aberrantly spliced transcript of MAP3K7. Treatment of HEK293T cells transfected with the K700E-mutated SF3B1 with cycloheximide leads to increased accumulation of the aberrant spliced transcript of MAP3K7, demonstrating that the aberrantly spliced transcript of MAP3K7 is targeted by nonsense-mediated decay. The aberrantly spliced MAP3K7 transcript uses an aberrant 3' splice sites and an alternative branchpoint sequence. In addition, the aberrant splicing of MAP3K7 requires not only the polypyrimidine tract associated with normal splicing but also an alternative polypyrimidine tract upstream of the aberrant 3' splice site. Other cancer-associated SF3B1 mutations also cause the aberrant splicing of MAP3K7, which depends on the same sequence features. Our data provide a further understanding of the mechanisms underlying aberrant splicing induced by cancer-associated SF3B1 mutation, and reveal an important role of alternative polypyrimidine tract in diseases.
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http://dx.doi.org/10.1093/jb/mvab023DOI Listing
March 2021

Detection of organophosphorus pesticides by nanogold/mercaptomethamidophos multi-residue electrochemical biosensor.

Food Chem 2021 Aug 9;354:129511. Epub 2021 Mar 9.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address:

Based on the successful synthesis of mercaptomethamidophos as a substrate, a novel nanogold/mercaptomethamidophos multi-residue electrochemical biosensor was designed and fabricated by combining nanoscale effect, strong Au-S bonds as well as interaction between acetylcholinesterase (AChE) and mercaptomethamidophos, which can simultaneously detect 11 kinds of organophosphorus pesticides (OPPs) and total amount of OPPs using indirect competitive method. Electrochemical behavior of the modified electrode was characterized by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The AChE concentration and incubation time were optimized at 37.4 °C to achieve the best detection effect. This biosensor exhibits excellent electrochemical properties with a wider linear range of 0.1 ~ 1500 ng·mL, lower detection limit of 0.019 ~ 0.077 ng·mL, better stability and repeatability, which realizes the rapid detection of total amount of OPPs, and can simultaneously detect a large class of OPPs rather than one kind of OPP. Two OPPs (trichlorfon, dichlorvos) were detected in actual samples of apple and cabbage and achieved satisfactory test results.
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http://dx.doi.org/10.1016/j.foodchem.2021.129511DOI Listing
August 2021

Kerr-nonlinearity induced bistable-like parity-time phase transition in coupled waveguides.

Opt Express 2021 Mar;29(5):7935-7947

We analyze the parity-time () symmetric phase in coupled two waveguides with a Kerr-type medium in between. Paying attention to the emitted field from a dipole source inside, we show that when the strength of the dipole increases, the optical Kerr effect can render a phase transition from the exact phase to the broken phase. Furthermore, a salient phenomenon of bistable-like phase is observed, in which the emitted field possesses a paradox between the two kinds of phases. We show that the physical mechanism of this bistable-like phenomenon is a globally inhomogeneous phase, in which different spatial regions of the whole structure can possess different phases (broken or exact). This study highlights the potential to manipulate the phase transition by using optical nonlinearity for many interesting applications.
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http://dx.doi.org/10.1364/OE.414551DOI Listing
March 2021

Rationale and Application of PEGylated Lipid-Based System for Advanced Target Delivery of siRNA.

Front Pharmacol 2020 20;11:598175. Epub 2021 Jan 20.

Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

RNA interference (RNAi) technology has become a powerful tool in application of unraveling the mechanism of disease and may hold the potential to be developed for clinical uses. Small interfering RNA (siRNA) can bind to target mRNA with high specificity and efficacy and thus inhibit the expression of related protein for the purpose of treatment of diseases. The major challenge for RNAi application is how to improve its stability and bioactivity and therefore deliver therapeutic agents to the target sites with high efficiency and accuracy. PEGylated lipid-based delivery system has been widely used for development of various medicines due to its long circulating half-life time, low toxicity, biocompatibility, and easiness to be scaled up. The PEGylated lipid-based delivery system may also provide platform for targeting delivery of nucleic acids, and some of the research works have moved to the phases for clinical trials. In this review, we introduced the mechanism, major challenges, and strategies to overcome technical barriers of PEGylated lipid-based delivery systems for advanced target delivery of siRNA . We also summarized recent advance of PEGylated lipid-based siRNA delivery systems and included some successful research works in this field.
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http://dx.doi.org/10.3389/fphar.2020.598175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944141PMC
January 2021

Evidence for temporal relationship between the late Mesozoic multistage Qianlishan granite complex and the Shizhuyuan W-Sn-Mo-Bi deposit, SE China.

Sci Rep 2021 Mar 12;11(1):5828. Epub 2021 Mar 12.

Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang, 050061, China.

The world-class Shizhuyuan W-Sn-Mo-Bi deposit is spatially related to the Qianlishan granite complex (QGC) in Hunan Province, China. However, the age and classification of the QGC are still debated, and a better understanding of the temporal genetic relationship between the QGC and the Shizhuyuan deposit is essential. Here, we present chemical compositions the intrusive phases of the QGC and the results of detailed zircon U-Pb dating and muscovite Ar-Ar dating of a mineralized greisen vein. Our new zircon laser ablation inductively coupled plasma mass spectrometry U-Pb age data constrain the emplacement of the QGC to 155-151.7 Ma. According to petrological, geochemical and geochronological data and the inferred redox conditions, the QGC can be classified into four phases: P, porphyritic biotite granites; P, porphyritic biotite granites; P, equigranular biotite granite; and P, granite porphyry dikes. All phases, and especially P-P, have elevated concentrations of ore-forming metals and heat-producing elements (U, Th, K; volume heat-producing rate of 5.89-14.03 μWm), supplying the metal and heat for the metalogic process of the Shizhuyuan deposit. The Ar-Ar muscovite age (154.0 ± 1.6 Ma) of the mineralized greisen vein in the Shizhuyuan deposit is consistent with the emplacement time of the QGC, suggesting their temporal genetic relationship.
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http://dx.doi.org/10.1038/s41598-021-84902-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971011PMC
March 2021

Incidence of death from kidney diseases among cancer patients: a US population-based analysis.

Int Urol Nephrol 2021 Mar 8. Epub 2021 Mar 8.

Geriatrics Department, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, No.15 Jiefang Road, Fancheng District, Xiangyang, Hubei, China.

Background: The purpose of our study was to retrospectively analyze the characteristic of death from kidney diseases among cancer patients and to screen the risk factors associated with nephrotic death using data from the surveillance, epidemiology, and end results (SEER) database.

Methods: The information on cancer patients dying of kidney diseases was retrieved from the SEER database. Standardized mortality ratios (SMRs) were calculated using the US general population as reference. Univariate and multivariate Cox regression analyses were conducted to screen potential risk factors of death from kidney diseases.

Results: Data of 7,167,808 patients diagnosed with malignant tumors between 2000 and 2016 were collected. Of these, 25,903 patients died of kidney diseases. Compared to the general population, cancer patients were at an elevated risk of nephrotic death with an SMR of 3.17, and this risk continues to increase in recent years. The majority of deaths from kidney diseases occur in patients > 45-year-old diagnosed with cancer of prostate, colorectum, and breast. Additionally, cancer patients with diagnosis in recent years, older age at diagnosis, black race, higher grade, and poorer disease stage were more likely to die of kidney diseases are at higher risk of nephrotic death compared to other cancer survivors.

Conclusion: The risk of death from kidney diseases is increasing among cancer survivors recently. Nephrologists should be actively involved in certain facets of cancer treatment, and provide effective nephrology care, especially for elderly patients with black race, higher grade, and poorer disease stage.
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http://dx.doi.org/10.1007/s11255-021-02801-1DOI Listing
March 2021

Regulation of the endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer.

Cell Chem Biol 2021 Feb 23. Epub 2021 Feb 23.

Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA. Electronic address:

Attachment of the ubiquitin (UB) peptide to proteins via the E1-E2-E3 enzymatic machinery regulates diverse biological pathways, yet identification of the substrates of E3 UB ligases remains a challenge. We overcame this challenge by constructing an "orthogonal UB transfer" (OUT) cascade with yeast E3 Rsp5 to enable the exclusive delivery of an engineered UB (xUB) to Rsp5 and its substrate proteins. The OUT screen uncovered new Rsp5 substrates in yeast, such as Pal1 and Pal2, which are partners of endocytic protein Ede1, and chaperones Hsp70-Ssb, Hsp82, and Hsp104 that counteract protein misfolding and control self-perpetuating amyloid aggregates (prions), resembling those involved in human amyloid diseases. We showed that prion formation and effect of Hsp104 on prion propagation are modulated by Rsp5. Overall, our work demonstrates the capacity of OUT to deconvolute the complex E3-substrate relationships in crucial biological processes such as endocytosis and protein assembly disorders through protein ubiquitination.
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http://dx.doi.org/10.1016/j.chembiol.2021.02.005DOI Listing
February 2021

Prevalence of Bovine Tuberculosis in Yaks Between 1982 and 2020 in Mainland China: A Systematic Review and Meta-Analysis.

Vector Borne Zoonotic Dis 2021 Mar 1. Epub 2021 Mar 1.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bovine tuberculosis (bTB) is a major chronic bacterial disease in cattle and is the major economic and animal welfare issue in the world. Although the economic costs and public health safety risks associated with the disease are considerable, the overall epidemiology of the Chinese yak () bTB is unclear. To fully reveal the basic prevalence of yak bTB in different regions of China, we searched five databases including PubMed, Science Direct, CNKI (China National Knowledge Infrastructure), Wanfang and Chongqing VIP. Based on the incidence and prevalence of yak tuberculosis in China from 1982 to 2020, a meta-analysis of yak bTB in China was established for the first time. By formulating the search formula, 97 studies were searched in five databases. According to the established exclusion criteria and excluded comments and repeated and irrelevance research, we finally selected 19 cross-sectional studies, which showed the prevalence of bTB in Chinese yaks. Random-effect meta-regression model analysis showed that the estimated prevalence of 122,729 yaks in China was 1.0 (95% confidence interval [CI]: 0.0-1.0). The regional prevalence varies greatly, northwest China prevalence rate 0. 39% (95% CI: 0.2-0. 64) and southwest China prevalence rate 2.59% (95% CI: 1.94-3.34); in terms of province level, the prevalence was highest in Tibet 2.59% (95% CI: 1.94-3.34), followed by Xinjiang 2.36% (95% CI: 0.86-4.58), and Shanxi has the lowest 0.00% (95% CI: 0.00-0.98). This systematic review and meta-analysis identified the estimated prevalence of bTB in Chinese yaks and estimated the underlying factors associated with bTB, including geographic location, sampling year, age, and TB detection method. Provide evidence to plan corresponding disease control strategies for policymakers and to assess future economic risks accurately.
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http://dx.doi.org/10.1089/vbz.2020.2687DOI Listing
March 2021

Seroprevalence and Risk Factors of the Bluetongue Virus in Cattle in China From 1988 to 2019: A Comprehensive Literature Review and Meta-Analysis.

Front Vet Sci 2020 28;7:550381. Epub 2021 Jan 28.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bluetongue caused by the bluetongue virus (BTV) is a non-contagious and an insect-borne disease mainly affecting domestic and wild ruminants. Bluetongue in cattle is associated with vesicular lesions, weight loss, low milk production, and low reproductive capacity. It should not be ignored as it is associated with large economic losses to the livestock breeding industry in China. Although many studies have investigated bluetongue virus infection in cattle, no nationwide study on the prevalence of bluetongue virus infection in cattle from China has yet been conducted. This meta-analysis aimed to evaluate the seroprevalence and risk factors for bluetongue in cattle. We collected 50 publications from 1988 to 2019 through PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), VIP Chinese journal database, and Wanfang database. A total of the pooled bluetongue seroprevalence of 12.2% (5,332/87,472) in cattle was tested. The point estimate of bluetongue collected from 2001 to 2011 was 22.5% (95% CI: 1.2-58.9), which was higher than after 2012 (9.9%, 95% CI: 3.3-19.4). The analysis of the feeding model subgroup revealed that the seroprevalence of bluetongue was significantly higher ( < 0.05) among free-range cattle (22.5%; 95% CI: 7.7-42.3) than among cattle from intensive farming systems (1.8%; 95% CI: 0.0-6.7). The seroprevalence of bluetongue in different species showed significant variation ( < 0.05), with the highest seroprevalence of 39.8% (95% CI: 18.7-63.0) in buffalo and the lowest seroprevalence of 4.3% (95% CI: 1.2-9.0) in yak. In the zoogeographical division subgroup, the seroprevalence of bluetongue correlated positively within a certain range with the species distribution of . Our findings suggested that bluetongue was prevalent in cattle in China. In addition, the contact with sheep, other ruminants, or transmission media such as may increase the seroprevalence of bluetongue disease in cattle. It is necessary to carry out continuous monitoring of the bluetongue seroprevalence. Moreover, comprehensive and improved strategies and measures should be implemented to prevent and control the spread of bluetongue.
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http://dx.doi.org/10.3389/fvets.2020.550381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901971PMC
January 2021

[Neuroprotective effects of linagliptin on ischemia/reperfusion in mice].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 Sep;36(5):452-455

Department of Neurology, the Second Hospital of Lanzhou University, Lanzhou 730030, China.

To evaluate the neuroprotective effects of linagliptin, a dipeptidyl peptidase-4(DPP-4) inhibitor, on cerebral ischemia/reperfusion(I/R) injury in mice. BALB/c mice were randomly divided into Sham group, I/R group and linagliptin (2.5, 5 and 10 mg/kg) +I/R group, 8 mice in each group. The mice in the linagliptin group were administrated by gavage 3 weeks before I/R. I/R injury model was induced by MCAO, neurological deficit scores(=8) and infarct volume(=4) were assessed 24 h following reperfusion. Forty-eight hours following reperfusion, mice were euthanized, the contents of glutathione (GSH), malondialdehyde (MDA), phosphoinositide 3 kinase (PI3K), phosphoprotein kinase B (p-Akt) and rapamycin target protein (mTOR) in brain tissue were measured (=4). Compared with the I/R group, the neurological deficit score and infarct volume were significantly decreased in the linagliptin pretreatment group after 24 h reperfusion (<0.05); the MDA content in the brain was significantly decreased (<0.05), while the GSH, PI3K, p-Akt and mTOR levels were significantly increased (<0.05). This study proves that linagliptin exerted a neuroprotective effect in I/R mice, which may be mediated by activation of the PI3K/AKT/mTOR pathway.
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http://dx.doi.org/10.12047/j.cjap.6001.2020.096DOI Listing
September 2020

Intraocular Suture Looping Technique for Flapless Four-Point Refixation of Dislocated Intraocular Lenses.

J Ophthalmol 2021 8;2021:6648777. Epub 2021 Feb 8.

Department of Ophthalmology, Tenth People's Hospital Affiliated to Shanghai Tongji University School of Medicine, Shanghai 200072, China.

Purpose: To describe a flapless/grooveless technique for four-point refixation of a dislocated intraocular lens (IOL) with four fenestrated haptics.

Methods: An intraocular suture looping technique was performed with the assistance of two 27-gauge needles. A looping needle was passed into the eye through paracentesis and was used to loop both fenestrated haptics on the same side with an 8-0 polypropylene thread. A guiding needle was used to guide the looping needle out of the eye at the scleral fixation sites. After looping each pair of fenestrated haptics on nasal/temporal sides with 8-0 polypropylene sutures, the IOL was refixated by definitive knotting. The exterior suture ends were buried into the sclera without the creation of scleral flaps/grooves.

Results: The technique was employed in four eyes (three patients). The mean postoperative follow-up period was 13.8 ± 2.2 months. Postoperatively, the IOLs of all the eyes remained well positioned and stable. The postoperative visual acuities of all the eyes were improved. No suture erosion, hypotony, scleral atrophy, chronic inflammation, retinal tears, and/or detachments were observed within the follow-up period.

Conclusion: The present technique provides minimal surgical invasion for the transscleral refixation of a dislocated IOL with four fenestrated haptics.
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http://dx.doi.org/10.1155/2021/6648777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884157PMC
February 2021

XQ-1H promotes cerebral angiogenesis via activating PI3K/Akt/GSK3β/β-catenin/VEGF signal in mice exposed to cerebral ischemic injury.

Life Sci 2021 May 17;272:119234. Epub 2021 Feb 17.

State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address:

Stroke still ranks as a most lethal disease worldwide. Angiogenesis during the chronic phase of ischemic stroke can alleviate ischemic injury and attenuate neurological deficit. XQ-1H is a new compound derived from the structure modification of ginkgolide B, which exerts anti-inflammation and neuroprotection against cerebral ischemic injury during the acute or subacute phase. However, whether XQ-1H facilitates angiogenesis and neural functional recovery during the chronic phase remains unclear. This research was designed to explore whether XQ-1H promotes angiogenesis after ischemic stroke and to preliminarily elucidate the mechanism. In vitro, XQ-1H was found to facilitate proliferation, migration and tube formation in bEnd.3 cells. In vivo, XQ-1H raised the CD31 positive microvessel number and increased focal cerebral blood flow in mice exposed to cerebral ischemic injury, and improved the neurological function. Mechanism studies revealed that XQ-1H exerted angiogenesis promoting effect via the PI3K/Akt/GSK3β/β-catenin/VEGF signal pathway, which was reversed by LY294002 (the specific inhibitor of PI3K/Akt). In conclusion, XQ-1H exerts angiogenetic effect both in vivo and in vitro, which is a potential agent against ischemic stroke during chronic phase.
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http://dx.doi.org/10.1016/j.lfs.2021.119234DOI Listing
May 2021