Publications by authors named "Bo Yuan"

607 Publications

A Co-Expression Network Reveals the Potential Regulatory Mechanism of lncRNAs in Relapsed Hepatocellular Carcinoma.

Front Oncol 2021 31;11:745166. Epub 2021 Aug 31.

Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Background: The mechanistic basis for relapsed hepatocellular carcinoma (HCC) remains poorly understood. Recent research has highlighted the important roles of long non-coding RNAs (lncRNAs) in HCC. However, there are only a few studies on the association between lncRNAs and HCC relapse.

Methods: Differentially expressed lncRNAs and mRNAs between a primary HCC group and relapsed HCC group were identified using the edge R package to analyze the GSE101432 dataset. The differentially expressed lncRNAs and mRNAs were used to construct a lncRNA-mRNA co-expression network. Weighted gene co-expression network analysis followed by Gene Ontology (GO) enrichment analyses were conducted on the database. Furthermore, correlation and survival analyses were performed using The Cancer Genome Atlas database, and expression in the clinical samples was verified by qRT-PCR. Thereafter, we inputted the genes from the two groups into the HCC TNM stage and tumor grade database from TCGA. Finally, we performed Kaplan-Meier survival analysis on the lncRNAs related to relapsed HCC.

Results: In this study, lncRNAs and mRNAs associated with HCC relapse were identified. Two gene modules were found to be closely linked to this. The GO terms in the yellow and black modules were related to cell proliferation, differentiation, and survival, as well as some transcription-related biological processes. Through qRT-PCR, we found that the expression levels of LINC00941 and LINC00668 in relapsed HCC were higher than those in primary HCC. Further, mRNA levels of , , , , , and were changed in relapsed HCC compared to levels in primary HCC. In addition, we verified that these genes could predict the overall survival and recurrence-free survival of HCC. Moreover, we found that LINC00668 and LINC00941 could affect tumor grade and TNM stages. In total, we identified and validated two lncRNAs (LINC00941 and LINC00668) and six mRNAs (, , , , , ) associated with HCC relapse.

Conclusion: In summary, we identified the key gene modules and central genes associated with relapsed HCC and constructed lncRNA-mRNA networks related to this. These genes are likely to have potential prognostic value for relapsed HCC and might shed new light on novel biomarkers or diagnostic targets for relapsed HCC.
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http://dx.doi.org/10.3389/fonc.2021.745166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438305PMC
August 2021

Preoperative Short Physical Performance Battery as a predictor of prolonged hospitalization after coronary artery bypass grafting in older patients.

J Int Med Res 2021 Sep;49(9):3000605211044043

Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Objective: The study aimed to evaluate the predictive value of the Short Physical Performance Battery (SPPB) and its three components for identifying the requirement for a prolonged hospitalization for coronary artery bypass grafting (CABG) in older patients.

Methods: This prospective, observational study included 425 older patients who consecutively underwent selective CABG surgery. All patients were assessed for the SPPB. We analysed the association of the postoperative length of hospital stay with the SPPB and three components. Receiver operating characteristic curve analysis was performed to determine the validity of the SPPB for predicting older patients with a prolonged hospitalization (>8 days).

Results: The SPPB and its three individual components were independent risk factors for a prolonged postoperative length of hospital stay. Only the SPPB summary score and the 4-m gait speed components showed good discriminative capabilities. An SPPB score cut-off of 9.5 provided the best accuracy for identifying a prolonged hospitalization. A poor physical performance (SPPB <10 score) was related to an increased risk of adverse cardiac and cerebrovascular events within 6 months after discharge.

Conclusions: This study provides evidence for clinical utility of the SPPB score for identifying older patients undergoing CABG who are at risk of prolonged hospitalization.
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http://dx.doi.org/10.1177/03000605211044043DOI Listing
September 2021

Identification of CHMP4C as a new risk gene for inherited dilated cardiomyopathy.

J Genet Genomics 2021 Sep 12. Epub 2021 Sep 12.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Disease, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.08.014DOI Listing
September 2021

Development and validation of a micro-QuEChERS method with high-throughput enhanced matrix removal followed with UHPLC-QqQ-MS/MS for analysis of raspberry ketone-related phenolic compounds in adipose tissues.

Talanta 2021 Dec 13;235:122716. Epub 2021 Jul 13.

New Use Agriculture and Natural Plant Products Program, Department of Plant Biology and Center for Agriculture Food Ecosystems, Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ, 08901, USA; Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ, 08901, USA; Department of Medicinal Chemistry, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ, 08854, USA. Electronic address:

Raspberry ketone (RK) is a major flavor compound in red raspberries, and it has been marketed as a popular weight-loss dietary supplement with high potential in accumulating in fatty tissues. However, challenges in extracting and characterizing RK and its associated phenolic compounds in fatty tissues persist due to the complex matrix effect. In this work, we reported a high-throughput sample preparation method for RK and 25 related phenolic compounds in white adipose tissues using an improved micro-scale QuEChERS (quick, efficient, cheap, easy, rugged and safe) approach with enhanced matrix removal (EMR)-lipid cleanup in 96-well plates, followed by UHPLC-QqQ-MS/MS analysis. The absolute recovery was 73-105% at the extraction step, and achieved 71-96% at the EMR cleanup step. The EMR cleanup removed around 66% of total lipids in the acetonitrile extract as profiled by UHPLC-QTOF-MS/MS. The innovative introduction of a reversed-phase C18 sorbent into the extract significantly improved the analytes' recovery during SpeedVac drying. The final accuracy achieved 80-120% for most analytes. Overall, this newly developed and validated method could serve as a powerful tool for analyzing RK and related phenolic compounds in fatty tissues.
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http://dx.doi.org/10.1016/j.talanta.2021.122716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441007PMC
December 2021

Cytotoxic Effects of Hellebrigenin and Arenobufagin Against Human Breast Cancer Cells.

Front Oncol 2021 26;11:711220. Epub 2021 Aug 26.

Department of Applied Biochemistry, Tokyo University of Pharmacy & Life Sciences, Hachioji, Japan.

Development of new therapeutic strategies for breast cancer is urgently needed due to the sustained emergence of drug resistance, tumor recurrence and metastasis. To gain a novel insight into therapeutic approaches to fight against breast cancer, the cytocidal effects of hellebrigenin (Helle) and arenobufagin (Areno) were investigated in human estrogen receptor (ER)-positive breast cancer cell line MCF-7 and triple-negative breast cancer cell line MDA-MB-231. Helle exhibited more potent cytotoxicity than Areno in both cancer cells, and MCF-7 cells were more susceptible to both drugs in comparison with MDA-MB-231 cells. Apoptotic-like morphological characteristics, along with the downregulation of the expression level of Bcl-2 and Bcl-xL and the upregulation of the expression level of Bad, were observed in Helle-treated MCF-7 cells. Helle also caused the activation of caspase-8, caspase-9, along with the cleavage of poly(ADP-ribose) polymerase in MCF-7 cells. Helle-mediated necrosis-like phenotype, as evidenced by the increased propidium iodide (PI)-positive cells was further observed. G/M cell cycle arrest was also induced by Helle in the cells. Upregulation of the expression level of p21 and downregulation of the expression level of cyclin D1, cyclin E1, cdc25C and survivin were observed in MCF-7 cells treated with Helle and occurred in parallel with G/M arrest. Autophagy was triggered in MCF-7 cells and the addition of wortmannin or 3-MA, two well-known autophagy inhibitors, slightly but significantly rescued the cells. Furthermore, similar alterations of some key molecules associated with the aforementioned biological phenomena were observed in MDA-MB-231 cells. Intriguingly, the numbers of PI-positive cells in Helle-treated MCF-7 cells were significantly reduced by wortmannin and 3-MA, respectively. In addition, Helle-triggered G/M arrest was significantly corrected by wortmannin, suggesting autophagy induction contributed to Helle-induced cytotoxicity of breast cancer cells by modulating necrosis and cell cycle arrest. Collectively, our results suggested potential usefulness of both Helle and Areno in developing therapeutic strategies to treat patients with different types of breast cancer, especially ER-positive breast cancer.
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http://dx.doi.org/10.3389/fonc.2021.711220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427765PMC
August 2021

Leptin promotes glycolytic metabolism to induce dendritic cells activation via STAT3-HK2 pathway.

Immunol Lett 2021 Sep 1;239:88-95. Epub 2021 Sep 1.

Department of Immunology, College of Basic Medical Science, Dalian Medical University, Liaoning, China. Electronic address:

Leptin is over-secreted in many autoimmune diseases, which can promote dendritic cells (DCs) maturation and up-regulate the expression of inflammatory cytokines, but the underlying mechanisms are not fully elucidated. Considering the major role of leptin in maintaining energy balance and the significant role of glycolysis in DCs activation, our study aims to investigate whether leptin promotes the activation of DCs via glycolysis and its underlying mechanisms. We demonstrated that leptin promoted the activation of DCs, including up-regulating the expression of co-stimulatory molecules and inflammatory cytokines, enhancing the proliferation and T helper 17 (Th17) cell ratio in peripheral blood mononuclear cells (PBMC) co-cultured with leptin-stimulated DCs. Leptin also enhanced DCs glycolysis with increased glucose consumption, lactate production, and the expression of hexokinase 2 (HK2). In addition, the activation of DCs stimulated by leptin could be inhibited by the glycolysis inhibitor 2-deoxy-d-glucose (2-DG). To explore the signaling pathways involved in leptin-induced HK2 expression, we observed that the inhibitors of STAT3 (NSC74859) could repress the enhancement of HK2 triggered by leptin stimulation. Therefore, our results indicated that leptin promoted glycolytic metabolism to induce DCs activation via STAT3-HK2 pathway.
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http://dx.doi.org/10.1016/j.imlet.2021.08.006DOI Listing
September 2021

Differential effects of Huaier aqueous extract on human CD4T lymphocytes from patients with primary immune thrombocytopenia.

Exp Hematol 2021 Aug 24. Epub 2021 Aug 24.

Department of Hematology, Second Hospital of Dalian Medical University, Dalian, Liaoning, China; Department of Immunology, Dalian Medical University, Dalian, Liaoning, China. Electronic address:

Huaier, a traditional Chinese medicine, is currently used to treat certain types of cancer in the clinic and is also regarded as an immune-modulating and immune-enhancing agent that regulates immune cells. Emerging evidence indicates that an imbalance of immune cells, such as CD4 T helper (Th) lymphocytes, contributes to the progression of immune thrombocytopenia (ITP), but the effects of Huaier on the regulation of CD4 T cells are not yet fully elucidated. In the present study, Jurkat cells and peripheral blood mononuclear cells (PBMCs) from patients with ITP and healthy volunteers were treated with Huaier aqueous extract (HR). The CCK-8 assay revealed that HR suppressed the proliferation of Jurkat cells in a dose-dependent manner, whereas 3 mg/mL could decrease cell viability by 50%. At the latter concentration, the activation of CD4 T cells from patients with ITP was partially attenuated. In addition, HR could correct the unbalanced Th1/Th2 polarization and inhibit the secretion of pro-inflammatory factors interleukin (IL)-2, tumor necrosis factor-α, and interferon-γ. It also suppressed Treg and facilitated Th17 differentiation, but did not change the levels of IL-10 and transforming growth factor-β. Thus, this study provides more information on how Huaier regulates cellular immunity and improves our understanding of the use of Huaier in ITP.
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http://dx.doi.org/10.1016/j.exphem.2021.08.005DOI Listing
August 2021

Middle-Late Triassic sedimentary provenance of the southern Junggar Basin and its link with the post-orogenic tectonic evolution of Central Asia.

Sci Rep 2021 Aug 23;11(1):17041. Epub 2021 Aug 23.

Research Institute of Exploration and Development, Xinjiang Oilfield Company, PetroChina, Karamay, 834000, Xinjiang, China.

Due to the unknown Triassic volcanism in the Junggar Basin, the Middle-Late Triassic sedimentary provenance in the southern Junggar Basin (SJB) has long been controversial. Detrital zircon grains from 13 samples of the Middle-Upper Triassic Xiaoquangou Group in the SJB were analyzed using zircon U-Pb geochronology to constrain the provenance of Triassic sedimentary rocks and to further understand their source-to-sink system. Comparison of detrital zircon U-Pb age distributions for 13 samples reveals that the Triassic age populations predominate in sediments of the northern Bogda Mountains, with subordinate in the southern Bogda Mountains, and no or minimal in the North Tianshan (NTS). Coupled with sandstone petrological, sedimentary geochemical and paleocurrent data, the Triassic detrital zircon grains of the Xiaoquangou Group in the SJB were probably input from the Bogda Mountains. As Pennsylvanian and Mississippian zircon grains are mainly derived from the NTS and Central Tianshan (CTS), the provenance of the Xiaoquangou Group includes the NTS, CTS and Bogda Mountains. But the different samples in different sink areas have different provenances, originating from at least four source-to-sink systems. The supply of sediments from the Bogda Mountains started in the Late Triassic, suggesting initial uplift of the Bogda Mountains.
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http://dx.doi.org/10.1038/s41598-021-96455-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382705PMC
August 2021

Three-dimensional reduction method with a modified C2 isthmus screw in irreducible atlantoaxial dislocation: a technical note.

BMC Surg 2021 Aug 12;21(1):324. Epub 2021 Aug 12.

Spine Center, Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University (Second Military Medical University), Shanghai, 200003, People's Republic of China.

Background: Three-dimensional reduction plays a vital role in surgical reduction of irreversible atlantoaxial dislocation (IAAD). However, the most commonly used combination of C1 pedicle screw (PS) or lateral mass screw (LMS) and C2 PS or isthmus screw often fails to achieve satisfactory reduction at one time. The difficulty is usually caused by short anteroposterior and vertical distance between heads of C1 and C2 screws, which lack enough space for reduction operation. The objective of this study is to describe a three-dimensional reduction method with a modified C2 isthmus screw and to illustrate its advantage and effectiveness for IAAD.

Methods: Twelve patients with IAAD underwent reduction and fixation with modified C2 isthmus screw combined with C1 PS or LMS, fusion with autologous bone graft. The insertion point was lateral to the intersection of caudal edge of C2 lamina and lateral mass, with a trajectory towards C2 isthmus, via lateral mass. The three-dimensional reduction was achieved through pulling and distracting. Radiographic evaluation included anteroposterior and direct distance between different insertion points, the occipitoaxial angle (O-C2A), clivus-canal angle (CCA) and cervicomedullary angle (CMA). Clinical outcomes evaluation included the Japanese Orthopaedic Association (JOA) score, Visual analog scale (VAS) and Neck Disability Index (NDI).

Results: All the patients maintained effective reduction during the follow-up. The anteroposterior and direct distance was significantly higher in modified C2 isthmus screw than C2 PS whether combined with C1 PS or LMS (P < 0.05). The degree of O-C2A, CCA and CMA, JOA score, NDI, and VAS were significantly improved after the surgery (P < 0.05).

Conclusions: Three-dimensional reduction method with a modified C2 isthmus screw is effective and safe in managing IAAD. It can increase the anteroposterior and vertical distance between the heads of C1 and C2 screws, which is benefit for the three-dimensional reduction operation of IAAD.
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http://dx.doi.org/10.1186/s12893-021-01321-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362247PMC
August 2021

Cyclic Tensile Stress to Rat Thoracolumbar Ligamentum Flavum Inducing the Ossification of Ligamentum Flavum: An In Vivo Experimental Study.

Spine (Phila Pa 1976) 2021 Sep;46(17):1129-1138

Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University (Second Military Medical University), Shanghai, People's Republic of China.

Study Design: Western blot, reverse transcription-polymerase chain reaction (RT-PCR), radiological, and histological analyses of the rat ossification of ligamentum flavum (OLF) induced by cyclic tensile stress.

Objective: The aim of this study was to induce the OLF using cyclic tensile stress to rat thoracolumbar ligamentum flavum, and to investigate the possible molecular mechanism of tension-induced OLF.

Summary Of Background Data: Tensile stress has been considered as an important factor leading to the OLF. So far, however, no OLF induced by tension has been reported.

Methods: Forty rats were randomly divided into five equal groups. For control groups, the blank and anesthesia groups were not subjected to tension. For experimental groups, the 4-, 8-, and 12-week groups were subjected to cyclic tensile stress of ligamentum flavum after abdominal anesthesia for 4 weeks, 8 weeks, and 12 weeks, respectively, using an original stress apparatus for rats. The radiological and morphological changes of rat spine, as well as the protein and mRNA expressions of CD44, bone morphogenetic protein-2 (BMP-2), integrin β3, collagen protein type I (COL1), osteopontin (OPN), runt-related transcription factor 2 (RUNX-2), and vascular endothelial growth factor (VEGF), were concerned.

Results: The micro-CT showed OLF in the 4-, 8-, and 12-week group. The axial maximum occupied area of ossifications was 1.42 mm2, 3.35 mm2, and 7.28 mm2, respectively. In histopathology, chondrocytes proliferated in the experimental model; woven bone arose in the 8- and 12-week groups, and was more noticeable in the 12-week group. According to western blot and RT-PCR, the expressions of seven osteogenesis-related molecules were all increased in three experimental groups.

Conclusion: Cyclic tensile stress to the ligamentum flavum in rats can induce the OLF, and the longer the duration, the more visible the osteogenesis. The upregulation and synergism of osteogenesis-related molecules may contribute to the OLF induced by tensile stress.Level of Evidence: N/A.
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http://dx.doi.org/10.1097/BRS.0000000000004087DOI Listing
September 2021

Porous Materials Confining Single Atoms for Catalysis.

Front Chem 2021 21;9:717201. Epub 2021 Jul 21.

Institute of Atmospheric Environmental Management and Pollution Control, China University of Mining & Technology (Beijing), Beijing, China.

In recent years, single-atom catalysts (SACs) have received extensive attention due to their unique structure and excellent performance. Currently, a variety of porous materials are used as confined single-atom catalysts, such as zeolites, metal-organic frameworks (MOFs), or carbon nitride (CN). The support plays a key role in determining the coordination structure of the catalytic metal center and its catalytic performance. For example, the strong interaction between the metal and the carrier induces the charge transfer between the metal and the carrier, and ultimately affects the catalytic behavior of the single-atom catalyst. Porous materials have unique chemical and physical properties including high specific surface area, adjustable acidity and shape selectivity (such as zeolites), and are rational support materials for confined single atoms, which arouse research interest in this field. This review surveys the latest research progress of confined single-atom catalysts for porous materials, which mainly include zeolites, CN and MOFs. The preparation methods, characterizations, application fields, and the interaction between metal atoms and porous support materials of porous material confined single-atom catalysts are discussed. And we prospect for the application prospects and challenges of porous material confined single-atom catalysts.
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http://dx.doi.org/10.3389/fchem.2021.717201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333616PMC
July 2021

AMBRA1 promotes TGF-β signaling via non-proteolytic polyubiquitylation of Smad4.

Cancer Res 2021 Aug 6. Epub 2021 Aug 6.

Life Sciences Institute and Innovation Center for Cell Biology, Zhejiang University

Transforming growth factor-β (TGF-β) is pro-metastatic in advanced cancers and its biological activities are mainly mediated by the Smad family of proteins. Smad4 is the central signal transducer and transcription factor in the TGF-β pathway, yet the underlying mechanisms that govern transcriptional activities of Smad4 are not fully understood. Here, we show that AMBRA1, a member of the DDB1 and CUL4-associated factor (DCAF) family of proteins, serves as the substrate receptor for Smad4 in the CUL4-RING (CRL4) ubiquitin ligase complex. The CRL4-AMBRA1 ubiquitin ligase mediates non-proteolytic polyubiquitylation of Smad4 to enhance its transcriptional functions. Consequently, AMBRA1 potentiated TGF-β signaling and critically promoted TGF-β-induced epithelial-to-mesenchymal transition, migration, and invasion of breast cancer cells. Mouse models of breast cancer demonstrated that AMBRA1 promotes metastasis. Collectively, these results show that CRL4-AMBRA1 facilitates TGF-β-driven metastasis by increasing Smad4 polyubiquitylation, suggesting AMBRA1 may serve as a new therapeutic target in metastatic breast cancer.
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http://dx.doi.org/10.1158/0008-5472.CAN-21-0431DOI Listing
August 2021

Evaluation of Cloud 3D Printing Order Task Execution Based on the AHP-TOPSIS Optimal Set Algorithm and the Baldwin Effect.

Micromachines (Basel) 2021 Jul 6;12(7). Epub 2021 Jul 6.

Shandong Longli Electronic Co., Ltd., Linyi 276000, China.

Focusing on service control factors, rapid changes in manufacturing environments, the difficulty of resource allocation evaluation, resource optimization for 3D printing services (3DPSs) in cloud manufacturing environments, and so on, an indicator evaluation framework is proposed for the cloud 3D printing (C3DP) order task execution process based on a Pareto optimal set algorithm that is optimized and evaluated for remotely distributed 3D printing equipment resources. Combined with the multi-objective method of data normalization, an optimization model for C3DP order execution based on the Pareto optimal set algorithm is constructed with these agents' dynamic autonomy and distributed processing. This model can perform functions such as automatic matching and optimization of candidate services, and it is dynamic and reliable in the C3DP order task execution process based on the Pareto optimal set algorithm. Finally, a case study is designed to test the applicability and effectiveness of the C3DP order task execution process based on the analytic hierarchy process and technique for order of preference by similarity to ideal solution (AHP-TOPSIS) optimal set algorithm and the Baldwin effect.
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http://dx.doi.org/10.3390/mi12070801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305594PMC
July 2021

Multi-Omics Data Integration Analysis of an Immune-Related Gene Signature in LGG Patients With Epilepsy.

Front Cell Dev Biol 2021 16;9:686909. Epub 2021 Jul 16.

Department of Neurology, Hunan Aerospace Hospital, Changsha Medical University, Changsha, China.

Background: The tumor immune microenvironment significantly affects tumor occurrence, progression, and prognosis, but its impact on the prognosis of low-grade glioma (LGG) patients with epilepsy has not been reported. Hence, the purpose of this study is to explore its effect on LGG patients with epilepsy.

Methods: The data of LGG patients derived from the TCGA database. The level of immune cell infiltration and the proportion of 22 immune cells were evaluated by ESTIMATE and CIBERSORT algorithms, respectively. The Cox and LASSO regression analysis was adopted to determine the DEGs, and further established the clustering and risk score models. The association between genomic alterations and risk score was investigated using CNV and somatic mutation data. GSVA was adopted to identify the immunological pathways, immune infiltration and inflammatory profiles related to the signature genes. The Tumor Immune Dysfunction and Exclusion (TIDE) algorithm and GDSC database were used to predict the patient's response to immunotherapy and chemotherapy, respectively.

Results: The prognosis of LGG patients with epilepsy was associated with the immune score. Three prognostic DEGs (ABCC3, PDPN, and INA) were screened out. The expression of signature genes was regulated by DNA methylation. The clustering and risk score models could stratify glioma patients into distinct prognosis groups. The risk score was an independent predictor in prognosis, with a high risk-score indicating a poor prognosis, more malignant clinicopathological and genomic aberration features. The nomogram had the better predictive ability. Patients at high risk had a higher level of macrophage infiltration and increased inflammatory activities associated with T cells and macrophages. While the higher percentage of NK CD56bright cell and more active inflammatory activity associated with B cell were present in the low-risk patients. The signature genes participated in the regulation of immune-related pathways, such as IL6-JAK-STAT3 signaling, IFN-α response, IFN-γ response, and TNFA-signaling-via-NFKB pathways. The high-risk patients were more likely to benefit from anti-PD1 and temozolomide (TMZ) treatment.

Conclusion: An immune-related gene signature was established based on ABCC3, PDPN, and INA, which can be used to predict the prognosis, immune infiltration status, immunotherapy and chemotherapy response of LGG patients with epilepsy.
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http://dx.doi.org/10.3389/fcell.2021.686909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322853PMC
July 2021

Vascular and Collagen Target: A Rational Approach to Hypertrophic Scar Management.

Adv Wound Care (New Rochelle) 2021 Aug 10. Epub 2021 Aug 10.

Burns and Plastic Surgery Department, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.

Hypertrophic scarring is a challenging issue for patients and clinicians. The prevalence of hypertrophic scarring can be up to 70% after burns, and patients suffer from pain, itching, and loss of joint mobility. To date, the exact mechanisms underlying hypertrophic scar formation are unclear, and clinical options remain limited. Several studies have demonstrated that pathological scars are a type of hyperactive vascular response to wounding. Scar regression has been found to be accompanied by microvessel occlusion, which causes severe hypoxia, malnutrition, and endothelial dysfunction, suggesting the essential roles of microvessels in scar regression. Therefore, interventions that target the vasculature, such as intense pulsed light, pulsed dye lasers, vascular endothelial growth factor antibodies, and Endostar, represent potential treatments. In addition, the mass of scar-associated collagen is usually not considered by current treatments. However, collagen-targeted therapies such as fractional CO laser and collagenase have shown promising outcomes in scar treatment. Traditional modalities used in current clinical practice only partially target scar-associated microvessels or collagen. As a result, the effectiveness of current treatments is limited and is too often accompanied by undesirable side effects. The formation of scars in the early stage is mainly affected by microvessels, whereas the scars in later stages are mostly composed of residual collagen. Traditional therapies do not utilize specific targets for scars at different stages. Therefore, more precise treatment strategies are needed. Scars should be classified as either "vascular-dominant" or "collagen-dominant" before selecting a treatment. In this way, strategies that are vascular-targeted, collagen-targeted, or a combination thereof could be recommended to treat scars at different stages.
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http://dx.doi.org/10.1089/wound.2020.1348DOI Listing
August 2021

Trem1 mediates neuronal apoptosis via interaction with SYK after spinal cord ischemia-reperfusion injury.

Am J Transl Res 2021 15;13(6):6117-6125. Epub 2021 Jun 15.

Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University Shanghai, China.

Objective: This research aimed to study the impact and regulatory mechanism of Trem1 in spinal cord ischemia-reperfusion injury (SCIRI).

Method: Temporary aortic cross clamp followed by reperfusion was used to establish SCIRI mice model. Mice motion function was estimated by Basso, Beattie, Bresnahan (BBB) score. Spinal cord infract zone was analyzed by HE and TUNEL staining. High throughput sequencing was performed to explore potential target for SCIRI. N2a cells were used to simulate the pathophysiological process of SCIRI in vitro with oxygen-glucose-serum deprivation/restoration (OGSD/R). RT-PCR and Western blot were token to determine mRNA and protein expression levels. Knockdown of Trem1 was performed with siRNA transfection and shRNA adenovirus injection . The relationship between Trem1 and SYK was analyzed by immunoprecipitation and immunofluorescence.

Result: We observed that neuronal apoptosis of spinal cord was aggravated after SCIRI. Trem1 expression was dramatically upregulated as shown by high throughput sequencing, RT-PCR and Western blot results. Furthermore, Trem1 triggered apoptosis of N2a cells induced by OGSD/R, and knockdown of Trem1 by siRNAs blocked apoptosis via PI3K/AKT and NF-κB signaling pathway by interacting with SYK. In addition, we found that intrathecal injection of adenovirus with Trem1 shRNA could downregulate SYK and inhibit neuron apoptosis caused by SCIRI .

Conclusion: Trem1 interacts with SYK and mediates neuronal apoptosis via the PI3K/AKT and NF-κB signaling pathway. Trem1 may be a therapeutic candidate for patients with SCIRI.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290738PMC
June 2021

Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping.

Biotechnol Biofuels 2021 Jul 23;14(1):162. Epub 2021 Jul 23.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.

Background: As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays.

Results: In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL and 13.58 U mL along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography-mass spectrometry (GC-MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions.

Conclusions: Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.
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http://dx.doi.org/10.1186/s13068-021-02011-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299586PMC
July 2021

Identification of Dihydromyricetin and Metabolites in Serum and Brain Associated with Acute Anti-Ethanol Intoxicating Effects in Mice.

Int J Mol Sci 2021 Jul 12;22(14). Epub 2021 Jul 12.

Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ 08854, USA.

Dihydromyricetin is a natural bioactive flavonoid with unique GABA receptor activity with a putative mechanism of action to reduce the intoxication effects of ethanol. Although dihydromyricetin's poor oral bioavailability limits clinical utility, the promise of this mechanism for the treatment of alcohol use disorder warrants further investigation into its specificity and druggable potential. These experiments investigated the bioavailability of dihydromyricetin in the brain and serum associated with acute anti-intoxicating effects in C57BL/6J mice. Dihydromyricetin (50 mg/kg IP) administered 0 or 15-min prior to ethanol (PO 5 g/kg) significantly reduced ethanol-induced loss of righting reflex. Total serum exposures (AUC024) of dihydromyricetin (PO 50 mg/kg) via oral (PO) administration were determined to be 2.5 µM × h (male) and 0.7 µM × h (female), while intraperitoneal (IP) administration led to 23.8-fold and 7.2- increases in AUC024 in male and female mice, respectively. Electrophysiology studies in α5β3γ2 GABA receptors expressed in Xenopus oocytes suggest dihydromyricetin (10 µM) potentiates GABAergic activity (+43.2%), and the metabolite 4-O-methyl-dihydromyricetin (10 µM) negatively modulates GABAergic activity (-12.6%). Our results indicate that administration route and sex significantly impact DHM bioavailability in mice, which is limited by poor absorption and rapid clearance. This correlates with the observed short duration of DHM's anti-intoxicating properties and highlights the need for further investigation into mechanism of DHM's potential anti-intoxicating properties.
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http://dx.doi.org/10.3390/ijms22147460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307506PMC
July 2021

Knockdown of lysyl oxidase like 1 inhibits the proliferation and pro-fibrotic effects of Transforming growth factor-β1-induced hypertrophic scar fibroblasts.

Can J Physiol Pharmacol 2021 Jul 20. Epub 2021 Jul 20.

Ningbo University, 47862, Department of Dermatology, Ningbo, Zhejiang Province, China;

Background: The excessive healing response during wound repair can result in hypertrophic scars (HS). Lysyl oxidase like 1 (LOXL1) has been reported to be associated with fibrosis via targeting TGF-β1 signaling. This study aimed to investigate the effect of LOXL1 on HS formation.

Methods: The expression of LOXL1 in HS tissues and TGF-β1-induced HSFs was detected via RT-qPCR and western blot. LOXL1 was silenced in HSFs using transfection with short hairpin RNA (shRNA), then wound healing process including cell proliferation, cell cycle distribution, migration and extracellular matrix deposition along with Smad expression were measured by CCK-8, EdU staining, flow cytometry, transwell, immunofluorescence and western blot assays.

Results: LOXL1 was up-regulated in HS tissues and TGF-β1-induced HSFs. Knockdown of LOXL1 inhibited proliferation and migration, but promoted cell cycle G0/G1 phase arrest in TGF-β1-induced HSFs. The increased expression of cyclin D1, CDK4, MMP2, MMP9, COL1A1, COL1A2, fibronectin, COL3A1, α-SMA, but decreased expression of p27, and the phosphorylation of Smad2 and Smad3 caused by TGF-β1 were also blocked by LOXL1 silence.

Conclusions: Silence of LOXL1 could effectively inhibit TGF-β1-induced proliferation, migration and ECM deposition in HSFs via inactivating Smad pathway. Targeting LOXL1 may have future therapeutic implications for HS treatment.
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http://dx.doi.org/10.1139/cjpp-2021-0242DOI Listing
July 2021

Effect of misalignment at different orientations associated with angle κ on optical performance of aspheric intraocular lenses with different surface designs.

Appl Opt 2021 Jul;60(20):5917-5924

Intraocular lens (IOL) misalignment and angle have had significant negative impact on post-operative quality of vision. ZEMAX software has been used to imitate the optical performance of pseudophakic eyes with different IOL surface designs at different orientations of IOL misalignment (decentration of 0.4 mm and tilt of 7°, and with the existence of 0.5 mm angle ). We found that an aspheric balanced curve optic surface maintained better optical performance via inducing less coma aberration. Coma aberration played an unexpectedly important role in the optical performance. With angle , the impact of IOL misalignment on visual quality was associated with the orientation of decentration and tilt, indicating that the coma compensation also took effect in pseudophakic eyes. Due to the high incidence of post-operative IOL misalignment, our results provide evidence of the importance of considering personalized angle before cataract surgery for patients.
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http://dx.doi.org/10.1364/AO.423000DOI Listing
July 2021

Molecular Markers of MDR of Chemotherapy for HSCC: Proteomic Screening With High-Throughput Liquid Chromatography-Tandem Mass Spectrometry.

Front Oncol 2021 28;11:687320. Epub 2021 Jun 28.

Department of Otolaryngology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Hypopharyngeal squamous cell cancer (HSCC) is a head and neck tumor with a poor prognosis. Chemotherapy lacks effectiveness because of multidrug resistance (MDR), which has increased toxic side effects. Thus, there is an urgent need to identify the molecular markers of MDR of chemotherapy for HSCC.

Methods: Fifty clinical samples of HSCC were derived from patients including 12 sensitive or resistant to chemotherapy drugs. Proteomic screening was performed using liquid chromatography-tandem mass spectrometry (LC-MS), which was based on data-independent acquisition. Molecular markers of MDR of chemotherapy in patients with HSCC were identified with clinical data and validated with ELISA.

Results: A total of 673 differentially expressed proteins were identified in HSCC samples, where 172 were upregulated and 501 were downregulated. A total of 183 differentially expressed proteins including 102 upregulated and 81 downregulated proteins, were identified by comparing cancer sensitive to chemotherapy with cancer resistant to chemotherapy. Clinical HSCC samples had significantly higher expression of FADD and significantly lower expression of RIPK1. Expressions of FADD and RIPK1 proteins were significantly lower in the chemotherapy-sensitive group. These expression differences were not correlated with clinical data. RIPK1 and FADD are involved in necroptosis and the signaling pathway of PRRs. Using ELISA, the low expression of RIPK1 and FADD was found in the patients sensitive to chemotherapy.

Conclusion: LC-MS proteomics is an effective method to identify the molecular markers of HSCC. FADD and RIPK1 can act as molecular markers of MDR of chemotherapy in patients with HSCC and may function through necroptosis and the PRR signaling pathway.
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http://dx.doi.org/10.3389/fonc.2021.687320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274423PMC
June 2021

Efficacy of Anlotinib for the Treatment of Angiosarcoma of the Face and Neck: A Case Report.

Front Oncol 2021 28;11:596732. Epub 2021 Jun 28.

Department of Medical Oncology, Chongqing University Three Gorges Hospital, Chongqing, China.

Angiosarcoma of the face and neck is a rare soft tissue sarcoma with a high degree of malignancy. The current treatment methods mainly rely on a combination of surgery and radiotherapy and/or chemotherapy. However, the options for drug treatment are very limited and surgery can be difficult to carry out due to the location of the tumor, so the efficacy of first-line drugs needs to be constantly explored. A case of angiosarcoma of the head and face diagnosed by biopsy is reported here. The patient received an oral anlotinib hydrochloride capsule once a day (12 mg on days 1 - 14/1 week off for a 21-day cycle) due to the difficulty of surgery. Until now (April, 2020), after 10 months of treatment, the patient's scalp and facial lesions have gradually reduced and the partial response and progression-free survival of this patient were good, with moderate or tolerable adverse events. This approach provides a new approach for the clinical treatment of malignant angiosarcoma of the face and neck with anlotinib as first-line therapy.
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http://dx.doi.org/10.3389/fonc.2021.596732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273654PMC
June 2021

Potential and mechanism of glomalin-related soil protein on metal sequestration in mangrove wetlands affected by aquaculture effluents.

J Hazard Mater 2021 Jun 27;420:126517. Epub 2021 Jun 27.

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China. Electronic address:

Aquaculture effluent discharge containing heavy metals affects estuarine mangrove wetlands. Glomalin-related soil protein (GRSP) is recalcitrant organic matter that can be trapped in mangrove wetlands and is critical to metal sequestration. However, studies on the effects of long-term aquaculture effluents on metal pollution in adjacent mangrove wetlands and the ecological role of GRSP are lacking. For the first time, we revealed the effects of discharge histories (0, 8, and 14 years) of shrimp pond effluents on metals (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn), including the entire process from feed to metals binding with GRSP in mangrove soils. Results showed that mangrove soils receiving the effluents generally had higher or similar metal loadings compared to the control, and long-term effluent discharge increased the potential toxicity of the metals. Aquaculture feed could be a main source of metal input. Redundancy analysis indicated that 14-year effluent discharge increased the pH, bulk density, total nitrogen, and total phosphorus of mangrove soils, reducing the potential of GRSP-bound metals. Scanning electron microscopy and infrared spectroscopy characterisation revealed that effluent disturbances changed the surface morphology and functional group contents of GRSP. This study provides insights into using GRSP as an aquaculture pollution bioindicator.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126517DOI Listing
June 2021

Perturbations in nitric oxide homeostasis promote Arabidopsis disease susceptibility towards Phytophthora parasitica.

Mol Plant Pathol 2021 Sep 9;22(9):1134-1148. Epub 2021 Jul 9.

The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, China.

Phytophthora species can infect hundreds of different plants, including many important crops, causing a number of agriculturally relevant diseases. A key feature of attempted pathogen infection is the rapid production of the redox active molecule nitric oxide (NO). However, the potential role(s) of NO in plant resistance against Phytophthora is relatively unexplored. Here we show that the level of NO accumulation is crucial for basal resistance in Arabidopsis against Phytophthora parasitica. Counterintuitively, both relatively low or relatively high NO accumulation leads to reduced resistance against P. parasitica. S-nitrosylation, the addition of a NO group to a protein cysteine thiol to form an S-nitrosothiol, is an important route for NO bioactivity and this process is regulated predominantly by S-nitrosoglutathione reductase 1 (GSNOR1). Loss-of-function mutations in GSNOR1 disable both salicylic acid accumulation and associated signalling, and also the production of reactive oxygen species, leading to susceptibility towards P. parasitica. Significantly, we also demonstrate that secreted proteins from P. parasitica can inhibit Arabidopsis GSNOR1 activity.
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http://dx.doi.org/10.1111/mpp.13102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359001PMC
September 2021

Bioinformatics Analysis of GFAP as a Potential Key Regulator in Different Immune Phenotypes of Prostate Cancer.

Biomed Res Int 2021 17;2021:1466255. Epub 2021 Jun 17.

Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Tumor immune escape plays an essential role in both cancer progression and immunotherapy responses. For prostate cancer (PC), however, the molecular mechanisms that drive its different immune phenotypes have yet to be fully elucidated. Patient gene expression data were analyzed from The Cancer Genome Atlas-prostate adenocarcinoma (TCGA-PRAD) and the International Cancer Genome Consortium (ICGC) databases. We used a Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) analysis and an unsupervised clustering analysis to identify patient subgroups with distinct immune phenotypes. These distinct phenotypes were then explored for associations for differentially expressed genes (DEGs) and both epigenetic and genetic landscapes. Finally, we used a protein-protein interaction analysis to identify key hub genes. We identified two patient subgroups with independent immune phenotypes associated with the expression of Programmed death-ligand 1 (PD-L1). Patient samples in Cluster 1 (1) had higher scores for immune-cell subsets compared to Cluster 2 (2), and 2 samples had higher specific somatic mutations, MHC mutations, and genomic copy number variations compared to 1. We also found additional cluster phenotype differences for DNA methylation, microRNA (miRNA) expression, and long noncoding RNA (lncRNA) expression. Furthermore, we established a 4-gene model to distinguish between clusters by integrating analyses for DEGs, lncRNAs, miRNAs, and methylation. Notably, we found that glial fibrillary acidic protein (GFAP) might serve as a key hub gene within the genetic and epigenetic regulatory networks. These results improve our understanding of the molecular mechanisms underlying tumor immune phenotypes that are associated with tumor immune escape. In addition, GFAP may be a potential biomarker for both PC diagnosis and prognosis.
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http://dx.doi.org/10.1155/2021/1466255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225431PMC
June 2021

Separate and combined effects of 3D building features and urban green space on land surface temperature.

J Environ Manage 2021 Oct 23;295:113116. Epub 2021 Jun 23.

Faculty of Geomatics, Lanzhou Jiaotong University, Lanzhou, 730070, China; Gansu Provincial Engineering Laboratory for National Geographic State Monitoring, Lanzhou, 730070, China.

Deduction of urban green space (UGS) and the multidimensional growth of building have exacerbated the urban heat island (UHI). Yet thorough investigations into how 3D building features and UGS combinedly influence urban land surface temperature (LST) are limited, especially at the road-based blocks scale. Therefore, the study uses the boosted regression tree (BRT) model to explore the relative contribution and marginal effects of the influential factors on LST, and quantify the warming/cooling effects of buildings and UGS. Results show that, (1) building coverage ratio (BCR) is the most influential factor among seven building metrics with a relative contribution of 44.6%. Besides, high-rise buildings tend to alleviate LST while low- and mid-rise buildings heat the surroundings. (2) Green coverage ratio (GCR), edge density (ED), and patch density (PD) are the most influential factors among six UGS metrics, with the relative contribution of 21.0%, 20.9%, and 20.4%, respectively. (3) Comprehensively considering 13 metrics, we find that the dominant influential factor is BCR with a relative contribution of 28.3%, while the regulation amplitudes to LST of aggregation index (AI) and GCR dramatically reduced. These findings indicate that the cooling effect of UGS will be obscured when the buildings coverage is large. Hence, only relying on UGS to alleviate the heat island effect seems inadequate, the keys are the reasonable planning and optimization of 3D built environment.
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http://dx.doi.org/10.1016/j.jenvman.2021.113116DOI Listing
October 2021

Learning From a Complementary-Label Source Domain: Theory and Algorithms.

IEEE Trans Neural Netw Learn Syst 2021 Jun 17;PP. Epub 2021 Jun 17.

In unsupervised domain adaptation (UDA), a classifier for the target domain is trained with massive true-label data from the source domain and unlabeled data from the target domain. However, collecting true-label data in the source domain can be expensive and sometimes impractical. Compared to the true label (TL), a complementary label (CL) specifies a class that a pattern does not belong to, and hence, collecting CLs would be less laborious than collecting TLs. In this article, we propose a novel setting where the source domain is composed of complementary-label data, and a theoretical bound of this setting is provided. We consider two cases of this setting: one is that the source domain only contains complementary-label data [completely complementary UDA (CC-UDA)] and the other is that the source domain has plenty of complementary-label data and a small amount of true-label data [partly complementary UDA (PC-UDA)]. To this end, a complementary label adversarial network (CLARINET) is proposed to solve CC-UDA and PC-UDA problems. CLARINET maintains two deep networks simultaneously, with one focusing on classifying the complementary-label source data and the other taking care of the source-to-target distributional adaptation. Experiments show that CLARINET significantly outperforms a series of competent baselines on handwritten digit-recognition and object-recognition tasks.
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http://dx.doi.org/10.1109/TNNLS.2021.3086093DOI Listing
June 2021

Bioconcentration of Several Series of Cationic Surfactants in Rainbow Trout.

Environ Sci Technol 2021 07 16;55(13):8888-8897. Epub 2021 Jun 16.

Department of Environmental Science, Stockholm University, Stockholm SE-106 91, Sweden.

Cationic surfactants have a strong affinity to sorb to phospholipid membranes and thus possess an inherent potential to bioaccumulate, but there are few measurements of bioconcentration in fish. We measured the bioconcentration of 10 alkylamines plus two quaternary ammonium compounds in juvenile rainbow trout at pH 7.6, and repeated the measurements at pH 6.2 for 6 of these surfactants. The BCF of the amines with chain lengths ≤ C was positively correlated with chain length, increasing ∼0.5 log units per carbon. Their BCF was also pH dependent and approximately proportional to the neutral fraction of the amine in the water. The BCFs of the quaternary ammonium compounds showed no pH dependence and were >2 orders of magnitude less than for amines of the same chain length at pH 7.6. This indicates that systemic uptake of permanently charged cationic surfactants is limited. The behavior of the quaternary ammonium compounds and the two C amines studied was consistent with previous observations that these surfactants accumulate primarily to the gills and external surfaces of the fish. At pH 7.6 the BCF exceeded 2000 L kg for 4 amines with chains ≥ C, showing that bioconcentration can be considerable for some longer chained cationic surfactants.
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http://dx.doi.org/10.1021/acs.est.1c02063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277129PMC
July 2021

Spatial-temporal dynamics and influencing factors of archaeal communities in the sediments of Lancang River cascade reservoirs (LRCR), China.

PLoS One 2021 15;16(6):e0253233. Epub 2021 Jun 15.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, China.

The spatial and temporal distribution of the archaeal community and its driving factors in the sediments of large-scale regulated rivers, especially in rivers with cascade hydropower development rivers, remain poorly understood. Quantitative PCR (qPCR) and Illumina MiSeq sequencing of the 16S rRNA archaeal gene were used to comprehensively investigate the spatiotemporal diversity and structure of archaeal community in the sediments of the Lancang River cascade reservoirs (LRCR). The archaeal abundance ranged from 5.11×104 to 1.03×106 16S rRNA gene copies per gram dry sediment and presented no temporal variation. The richness, diversity, and community structure of the archaeal community illustrated a drastic spatial change. Thaumarchaeota and Euryyarchaeota were the dominant archaeal phyla in the sediments of the cascade rivers, and Bathyarchaeota was also an advantage in the sediments. PICRUSt metabolic inference analysis revealed a growing number of genes associated with xenobiotic metabolism and carbon and nitrogen metabolism in downstream reservoirs, indicating that anthropogenic pollution discharges might act as the dominant selective force to alter the archaeal communities. Nitrate and C/N ratio were found to play important roles in the formation of the archaeal community composition. In addition, the sediment archaeal community structure was also closely related to the age of the cascade reservoir and hydraulic retention time (HRT). This finding indicates that the engineering factors of the reservoir might be the greatest contributor to the archaeal community structure in the LRCR.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253233PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205147PMC
June 2021

CircPVT1 promotes proliferation of lung squamous cell carcinoma by binding to miR-30d/e.

J Exp Clin Cancer Res 2021 Jun 10;40(1):193. Epub 2021 Jun 10.

School of Medicine, Southern University of Science and Technology, 1088th Xueyuan Road, Shenzhen, 518055, People's Republic of China.

Background: Circular RNAs (circRNAs) are a new type of extensive non-coding RNAs that regulate the activation and progression of different human diseases, including cancer. However, information on the underlying mechanisms and clinical significance of circRNAs in lung squamous cell carcinoma (LUSC) remains scant.

Methods: The expression profile of RNAs in 8 LUSC tissues, and 9 healthy lung tissues were assayed using RNA sequencing (RNA-seq) techniques. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to profile the expression of circPVT1 and its relationship with the prognosis of LUSC, i.e., survival analysis. Moreover, in vitro and in vivo experiments were performed to evaluate the impacts of circPVT1 on the growth of tumors. RNA pull-down tests, mass spectrometry, dual-luciferase reporter assessment, and RNA immune-precipitation tests were further conducted to interrogate the cross-talk between circPVT1, HuR, or miR-30d/e in LUSC.

Results: Our data showed that circPVT1 was upregulated in LUSC tissues, serum, and cell lines. LUSC patients with higher circPVT1 expression exhibited shorter survival rates. The in vivo and in vitro data revealed that circPVT1 promotes the proliferation of LUSC cells. Additionally, mechanistic analysis showed that HuR regulated circPVT1. On the other hand, circPVT1 acted as a competing endogenous RNA (ceRNA) of miR-30d and miR-30e in alleviating the suppressive influences of miR-30d and miR-30e on its target cyclin F (CCNF).

Conclusion: CircPVT1 promotes LUSC progression via HuR/circPVT1/miR-30d and miR-30e/CCNF cascade. Also, it acts as a novel diagnostic biomarker or treatment target of individuals diagnosed with LUSC.
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http://dx.doi.org/10.1186/s13046-021-01976-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194141PMC
June 2021
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