Publications by authors named "Bo Yu"

1,560 Publications

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Risk and sources of heavy metals and metalloids in dust from university campuses: A case study of Xi'an, China.

Environ Res 2021 Jul 17;202:111703. Epub 2021 Jul 17.

Department of Environmental Science, School of Geography and Tourism, Shaanxi Normal University, Xi'an, 710119, China.

College students study and live at university for several years; however, the pollution levels, ecological health risks, and sources of heavy metals and metalloids (HMMs) in the dust found at university campuses are still unknown. In this study, dust samples from university campuses in Xi'an were collected and the Zn, Mn, As, Pb, V, Cr, Co, Cu, Ba, and Ni contents were measured using X-ray fluorescence spectrometry. The pollution levels and ecological health risks of these HMMs were evaluated using the geo-accumulation, pollution load, and potential ecological risk indices and a health risk assessment model while their sources were apportioned using positive matrix factorization. The mean HMM concentrations in the dust were higher than the corresponding background values in the topsoil of Shaanxi Province. The Mn, V, Co, As, and Ni concentrations in the dust samples analyzed were within the levels categorized as no pollution by the geo-accumulation index standard, whereas other HMMs caused pollution to different degrees. Assessment of the pollution load index indicated that the dust samples analyzed were moderate contamination with HMMs. Pb and Cu in the dust presented considerable and moderate ecological risks, respectively; the other HMMs presented low ecological risks. The combined ecological risk of the HMMs measured in the dust samples was considerable. The non-carcinogenic and carcinogenic risks to male and female college students were within the safe levels. This study found three main sources of the HMMs measured in the dust: traffic, natural, and mixed sources (the latter including automobile repair industry waste and paints and pigments), which accounted for 47.5%, 29.3%, and 23.2% of the total HMM concentration, respectively.
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http://dx.doi.org/10.1016/j.envres.2021.111703DOI Listing
July 2021

Outcomes of Canaliculotomy with and without Silicone Tube Intubation in Management of Primary Canaliculitis.

Curr Eye Res 2021 Jul 19:1-4. Epub 2021 Jul 19.

Department of Orbital and Oculoplastic Surgery, Eye Hospital of Wenzhou Medical University, Wenzhou, China.

Purpose: To compare the outcomes of canaliculotomy and curettage with and without silicone tube intubation in the treatment of primary canaliculitis.

Methods: A prospective, randomized, interventional case series was performed. Fifty patients diagnosed with unilateral inferior primary canaliculitis underwent canaliculotomy with curettage and were randomly divided into two groups depending on silicone tube intubation. Twenty-five patients were recruited in group A (without intubation) and 25 patients were recruited in group B (with intubation). The resolution of infection, the success rate and postoperative complications observed in both groups were analyzed with a minimum follow-up of 1 year.

Results: Forty-seven patients were finally included in the study consisting of 23 patients in group A and 24 patients in group B. There were 33 females and 14 males with a median age of 57 ± 13.9 years (range 29-89 years). All patients recorded complete resolution of canaliculitis and no recurrent infections were observed in the 2 groups during follow-up. A significantly higher number of anatomical and functional successes were achieved in patients in group B (100%, 87.5%) than in group A (78.3%, 60.9%) ( < .05,). The surgical complication of canalicular obstruction was significantly higher in patients in group A (21.7%, 5/23) compared to group B (0/24)(P < .05).

Conclusion: Canaliculotomy with curettage gives excellent clinical outcomes in the treatment of patients with primary canaliculitis and a higher success rate can be achieved when silicone tube intubation is performed during the procedure. The use of silicone tube intubation may be a necessary choice in canaliculotomy to avoid post-operative canalicular obstruction.
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http://dx.doi.org/10.1080/02713683.2021.1942074DOI Listing
July 2021

Lipidomics Identified Lyso-Phosphatidylcholine and Phosphatidylethanolamine as Potential Biomarkers for Diagnosis of Laryngeal Cancer.

Authors:
Bo Yu Jizhe Wang

Front Oncol 2021 28;11:646779. Epub 2021 Jun 28.

Department of Otolaryngology Head and Neck Surgery, The Second Hospital of Dalian Medical University, Dalian, China.

Background: Laryngeal cancer (LaC) remains one of the most common tumors of the respiratory tract with higher incidence in men than in women. The larynx is a small but vital organ on the neck. The dysfunction of the larynx can cause serious health problems such as hoarseness, respiratory distress, and dysphonia. Many lipids (e.g. phospholipid, cholesterol, fatty acid) have been recognized as a crucial role in tumorigenesis. However, the lipid biomarkers are lacking and the lipid molecular pathogenesis of LaC is still unclear.

Methods: This study aims to identify new LaC-related lipid biomarkers used for the diagnosis or early diagnosis of LaC and to uncover their molecular characteristics. Thus, we conducted serum and tissue nontargeted lipidomics study from LaC patients (n = 29) and normal controls (NC) (n = 36) ultra-high performance liquid chromatography (UHPLC) coupled with high resolution mass spectrometry (HRMS). Multivariate and univariate statistics analyses were used to discriminate LaC patients from NC.

Results: As expected, a lipid panel including LPC (16:0) and PE (18:0p_20:4) was defined to distinguish the LaC patients from healthy individuals with very high diagnosis performance (area under the curve (AUC) value = 1.000, sensitivity value = 1.000, and specificity value = 1.000). In addition, the levels of Cer, CerG1, SM, PC, PC-O, PE, PI, PS, and ChE in the LaC group significantly increased as compared with the NC group. However, the levels of LPC, LPC-O, LPE, LPE-p, and DG in the LaC group significantly deceased when the one was compared with the NC group. Among significantly changed lipid species, lysophospholipids containing a palmitoyl chain or an arachidonic acid acyl chain remarkably decreased and phospholipids including a palmitoyl chain or an arachidonic acid acyl chain increased in the LaC patients.

Conclusion: Our results not only indicate that lipidomics is powerful tool to explore abnormal lipid metabolism for the laC, but suggest that lysophospholipids and phospholipids may serve as potential biomarkers for diagnosis of LaC.
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http://dx.doi.org/10.3389/fonc.2021.646779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273650PMC
June 2021

Thromboangiitis obliterans plasma-derived exosomal miR-223-5p inhibits cell viability and promotes cell apoptosis of human vascular smooth muscle cells by targeting VCAM1.

Ann Med 2021 12;53(1):1129-1141

Department of Vascular Surgery, Shanghai Pudong Hospital Affiliated to Fudan University, Shanghai, China.

Exosomes-encapsulated microRNAs (miRNAs) have been established to be implicated in the pathogenesis of different diseases. Nevertheless, circulating exosomal miRNAs of thromboangiitis obliterans (TAO) remains poorly understood. This study aimed to explore the effects of exosomal miRNAs associated with TAO on human vascular smooth muscle cells (HVSMCs). The exosomes were isolated from the plasma of TAO patients and normal controls and then were sent for small RNA sequencing. Differentially expressed miRNAs (DE-miRNAs) were identified by bioinformatics analysis and were confirmed by RT-qPCR. After that, PKH67 staining was used to label exosomes and co-cultured with HVSMCs. Cell viability and apoptosis were, respectively, tested by CCK-8 assay and flow cytometry. Finally, dual-luciferase reporter assay was used to confirm the downstream targets of miR-223-5p. A total of 39 DE-miRNAs were identified between TAO patients and normal controls, of which, miR-223-5p was one of the most significantly up-regulated miRNAs. TAO plasma-derived exosomes or miR-223-5p mimics inhibited cell viability of HVSMCs and promoted cell apoptosis. The pro-apoptotic effect of TAO plasma-derived exosomes was alleviated by miR-223-5p inhibitor. Additionally, the expressions of VCAM1 and IGF1R were down-regulated by exosomes and miR-223-5p mimics, and were abrogated by miR-223-5p inhibitor. Dual-luciferase report showed that VCAM1 was the target of miR-223-5p. Our findings imply that circulating exosomal miR-223-5p may play an essential role in the pathogenesis of TAO, and provide a basis for miR-6515-5p/VCAM1 as novel therapeutic targets and pathways for TAO treatment.
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http://dx.doi.org/10.1080/07853890.2021.1949487DOI Listing
December 2021

Leiomyomatosis peritonealis disseminata with endometriosis.

BMJ Case Rep 2021 Jul 13;14(7). Epub 2021 Jul 13.

Department of Obstetrics and Gynecology, Jeonbuk National University Medical School, Research Institute of Clincal Medicine of Jeonbuk National University-Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, The Republic of Korea

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http://dx.doi.org/10.1136/bcr-2020-240592DOI Listing
July 2021

Influences of Hydrogen Blending on the Joule-Thomson Coefficient of Natural Gas.

ACS Omega 2021 Jul 21;6(26):16722-16735. Epub 2021 Jun 21.

School of Mechanical Engineering, Beijing Key Laboratory of Pipeline Critical Technology and Equipment for Deepwater Oil and Gas Development, Beijing Institute of Petrochemical Technology, Beijing 102617, China.

Blending hydrogen into the natural gas pipeline is considered as a feasible way for large-scale and long-distance delivery of hydrogen. However, the blended hydrogen can exert major impacts on the Joule-Thomson (J-T) coefficient of natural gas, which is a significant parameter for liquefaction of natural gas and formation of natural gas hydrate in engineering. In this study, the J-T coefficient of natural gas at different hydrogen blending ratios is numerically investigated. First, the theoretical formulas for calculating the J-T coefficient of the natural gas-hydrogen mixture using the Soave-Redlich-Kwong (SRK) equation of state (EOS), Peng-Robinson EOS (PR-EOS), and Benedict-Webb-Rubin-Starling EOS (BWRS-EOS) are, respectively, derived, and the calculation accuracy is verified by experimental data. Then, the J-T coefficients of natural gas at six different hydrogen blending ratios and thermodynamic conditions are calculated and analyzed using the derived theoretical formulas and a widely used empirical formula. Results indicate that the J-T coefficient of the natural gas-hydrogen mixture decreases approximately linearly with the increase of the hydrogen blending ratio. When the hydrogen blending ratio reaches 30% (mole fraction), the J-T coefficient of the natural gas-hydrogen mixture decreases by 40-50% compared with that of natural gas. This work also provides a J-T coefficient database of a methane-hydrogen mixture with a hydrogen blending ratio of 5-30% at a pressure of 0.5-20 MPa and temperatures of 275, 300, and 350 K as a reference and a benchmark for interested readers.
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http://dx.doi.org/10.1021/acsomega.1c00248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264842PMC
July 2021

Electroacupuncture Attenuates Anxiety-Like Behaviors in a Rat Model of Post-traumatic Stress Disorder: The Role of the Ventromedial Prefrontal Cortex.

Front Neurosci 2021 24;15:690159. Epub 2021 Jun 24.

Department of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Electroacupuncture (EA) is a promising clinical approach to treating posttraumatic stress disorder (PTSD), yet the mechanisms whereby EA can alleviate anxiety and other PTSD symptoms have yet to be clarified. In the present report, rats underwent EA for 14 consecutive days following modified single prolonged stress (MSPS) exposure. These animals were then evaluated in open field and elevated plus maze tests (OFT and EPM), while Fos immunohistochemical staining was performed to assess ventromedial prefrontal cortex (vmPFC) functional activation. In addition, an extracellular recording and stimulation system was used to analyze vmPFC inputs into the ventral tegmental area (VTA) in these rats. Temporary vmPFC inactivation was further performed to assess whether this was sufficient to reverse the anxiolytic effects of EA. Overall, rats that underwent EA treatment spent more time in the central region (OFT) and the open arm (EPM) relative to MSPS model animals ( < 0.05). These MSPS model animals also exhibited significantly fewer activated Fos-positive nuclei in the vmPFC following behavioral testing, while EA was associated with a significant relative increase in c-Fos expression in this region. The transient inactivation of the vmPFC was sufficient to reverse the effects of EA treatment on anxiety-like behaviors in MSPS model rats. MSPS and SEA rats exhibiting no differences in bursting activity between baseline and vmPFC stimulation, whereas bursting activity rose relative to baseline upon ventral mPFC stimulation in EA treated and control rats. Together, these findings indicate that the vmPFC and its inputs into the VTA are functionally linked to the anxiolytic activity of EA, implicating this pathway in the EA-mediated treatment of PTSD.
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http://dx.doi.org/10.3389/fnins.2021.690159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264195PMC
June 2021

Polyphenols from blue honeysuckle (Lonicera caerulea var. edulis) berry inhibit lipid accumulation in adipocytes by suppressing lipogenesis.

J Ethnopharmacol 2021 Jul 8;279:114403. Epub 2021 Jul 8.

Department of Cardiology, 2nd Affiliated Hospital of Harbin Medical University, Harbin, 150001, PR China; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, 150001, PR China. Electronic address:

Ethnopharmacological Relevance: Blue honeysuckle (Lonicera caerulea var. edulis) berry has been used in folk medicine for the treatment of bacterial infections, gastrointestinal disorders, and metabolic diseases. There is evidence to support its pharmacological effects in improving diabetes, fatty liver, and obesity.

Aim Of Study: To investigate the effect of blue honeysuckle berry extract (BHBE) on lipid accumulation in adipocytes and the underlying mechanism.

Materials And Methods: High-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) was applied to analyze the polyphenolic compounds in BHBE. 3T3-L1 cells were used to induce into adipocytes. Oil Red O staining combined with triglyceride (TG) content determination were carried out to evaluate intracellular lipid accumulation. Western blot was used to determine the expression of lipogenic enzymes and transcription factors. Real-time PCR was used to determine the expression of lipolytic enzymes and adipocyte markers.

Results: The primary polyphenols in BHBE are flavonoids (mainly flavonols and anthocyanins). BHBE dose-dependently inhibited lipid accumulation in adipocytes by reducing the expression of fatty acid synthase (FAS) and increasing the phosphorylation level of acetyl-CoA carboxylase (ACC). Moreover, BHBE was found to promote the phosphorylation of AMP-activated protein kinase (AMPK) and further reduce the expression of lipogenic transcription factors (PPARγ, C/EBPα, and SREBP-1c), while the selective AMPK inhibitor attenuated the suppressive effect of BHBE on lipogenesis. In addition, BHBE increased the expression of beige adipocyte markers (Cd137 and Tmem26) and uncoupling protein 1 (UCP1) without affecting the expression of brown adipocyte markers (Ebf3 and Eva1).

Conclusion: BHBE inhibits lipid accumulation in adipocytes by suppressing lipogenesis via AMPK activation as well as by promoting beiging of adipocytes, which supports the anti-obesity potential of blue honeysuckle berry.
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http://dx.doi.org/10.1016/j.jep.2021.114403DOI Listing
July 2021

LncRNA SOX2OT alleviates mesangial cell proliferation and fibrosis in diabetic nephropathy via Akt/mTOR-mediated autophagy.

Mol Med 2021 Jul 8;27(1):71. Epub 2021 Jul 8.

Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, People's Republic of China.

Background: Accumulating evidences have demonstrated that long non-coding RNAs (lncRNAs) are involved in the pathophysiology of diabetic nephropathy (DN). lncRNA SOX2OT plays an essential role in many diseases, including diabetes. Herein, we aim to investigate the underlying mechanism of lncRNA SOX2OT in DN pathogenesis.

Methods: Streptozotocin-induced DN mouse models and high glucose-induced mouse mesangial cells were constructed to examine the expression pattern of lncRNA SOX2OT. The activation of autophagy was evaluated using immunohistochemistry, immunofluorescence and western blot analysis, respectively. SOX2OT overexpressing plasmid was applied to further verify the functional role of SOX2OT in DN pathogenesis. CCK-8 and EDU assays were performed to the proliferation of mesangial cells. Additionally, rapamycin, the inhibitor of mTOR signaling, was used to further clarify whether SOX2OT controls DN development through Akt/mTOR pathway.

Results: lncRNA SOX2OT was markedly down-regulated both in streptozotocin-induced DN mice and high glucose-induced mouse mesangial cells. Moreover, overexpression of lncRNA SOX2OT was able to diminish the suppression of autophagy and alleviate DN-induced renal injury. Functionally, CCK-8 and EDU assays indicated that lncRNA SOX2OT overexpression significantly suppressed the proliferation and fibrosis of mesangial cells. Additionally, an obvious inhibition of Akt/mTOR was also observed with lncRNA SOX2OT overexpression, which was then further verified in vivo.

Conclusion: In summary, we demonstrated that lncRNA SOX2OT alleviates the pathogenesis of DN via regulating Akt/mTOR-mediated autophagy, which may provide a novel target for DN therapy.
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http://dx.doi.org/10.1186/s10020-021-00310-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268185PMC
July 2021

Contralateral S1 nerve root transfer for motor function recovery in the lower extremity among patients with central nervous system injury: study protocol for a randomized controlled trial.

Ann Palliat Med 2021 Jun;10(6):6900-6908

Trauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Central nervous system injury (CNSI) comprises a series of common diseases that severely affect patients' motor function and quality of life and is associated with high disability and mortality rates. Previous studies have shown that contralateral lumbosacral nerve root transfer significantly improved the function of the paralyzed limb in rat models of CNSI. These studies showed that severing the sacral 1 nerve root (S1) did not damage the function of the ipsilateral lower extremity. Thus, we speculate that contralateral S1 nerve root transfer can improve the recovery of a paralyzed limb. Because no associated rigorously designed randomized controlled trial has evaluated the effectiveness of contralateral S1 nerve transfer thus far, we designed this clinical trial to compare the effects of this new treatment approach with those of traditional treatments in paralyzed patients after chronic CNSI.

Methods: This is a single-center, prospective, randomized controlled trial. Forty patients, who meet the inclusion criteria and have hemiplegia caused by chronic CNSI, will be randomly divided into the surgical or non-surgical group. The treatment effect in the 2 groups will be assessed before and 3, 6, 9, 12, 18, and 24 months after intervention by using numerous scales and resting-state functional magnetic resonance imaging. The primary outcome will be the Fugl-Meyer score for the lower limbs 24 months after treatment. The secondary outcomes include the modified Ashworth spasm scale, the modified Barthel scale, 10-m walking speed measurement results, three-dimensional gait analysis, muscle strength testing, electromyography, and resting-state functional magnetic resonance imaging findings. Safety outcomes and adverse events will be observed simultaneously.

Discussion: We expect that the surgery will improve the sensorimotor functions of the paralyzed limb, and the results of this trial will provide high-quality clinical evidence for a new efficient treatment strategy for disability after CNSI.

Trial Registration: Chinese Clinical Trial Registry: ChiCTR1800014414, registration date: 12 January 2018.
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http://dx.doi.org/10.21037/apm-21-183DOI Listing
June 2021

Luteolin prevents THP-1 macrophage pyroptosis by suppressing ROS production via Nrf2 activation.

Chem Biol Interact 2021 Aug 1;345:109573. Epub 2021 Jul 1.

Department of Cardiology, 2nd Affiliated Hospital of Harbin Medical University, Harbin 150001, PR China; Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin 150001, PR China. Electronic address:

Pyroptosis plays an important role in the pathogenesis of numerous infectious, autoimmune, and inflammatory diseases, which makes it a promising target for intervention. In this study, the effect of luteolin on pyroptosis and the underlying mechanism were investigated using the canonical NLRP3 inflammasome in THP-1 macrophages induced by LPS/ATP. The results showed that luteolin exhibited a potent preventive effect on THP-1 macrophage pyroptosis, as evidenced by the increase in cell viability and the decrease in LDH release. Moreover, luteolin was found to significantly reduce the expression of NLRP3, pro-CASP-1 and CASP-1, which are the key components of NLRP3 inflammasome, as well as the expression of N-GSDMD and IL-1β, and we proved that the inhibition of luteolin on NLRP3 inflammasome activation is ROS-dependent. Furthermore, it was demonstrated that luteolin promoted Nrf2 nuclear translocation, thereby increasing the expression of HO-1 that reduces ROS production, while the anti-pyroptotic effect of luteolin was reversed by a specific Nrf2 inhibitor. Additionally, luteolin inhibited NF-κB p65 phosphorylation and nuclear translocation. In summary, we conclude that luteolin prevents THP-1 macrophage pyroptosis by suppressing ROS production via Nrf2 activation as well as NF-κB inactivation. These results support luteolin as a potential bioactive chemical against pyroptosis-related inflammatory diseases.
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http://dx.doi.org/10.1016/j.cbi.2021.109573DOI Listing
August 2021

[Effects of zinc levels on synthesis and translocation of C-photoassimilates in leaves to fruit of apple during fruit expanding stage].

Ying Yong Sheng Tai Xue Bao 2021 Jun;32(6):2007-2013

College of Horticulture, Shenyang Agricultural University/Key Laboratory of Fruit Quality Development and Regulation of Liaoning Province, Shenyang 110866, China.

To explore the effects of zinc levels on the synthesis and translocation of photosynthetic products from leaves to fruits, and to lay a theoretical foundation for improving fruit quality through zinc supplementation during the critical period of apple fruit development, a field experiment was carried out with a eight-year old 'Hanfu'/GM256/ Borkh apple. We used the C tracer method to examine the effects of different zinc levels (ZnSO·HO 0, 0.1%, 0.2%, 0.3%, 0.4%, expressed by CK, Zn, Zn, Zn, Zn, respectively) on translocation of photosynthate to fruit during the stage of fruit expanding. The results showed that, with increasing zinc concentration, Rubisco enzyme activity, net photosynthetic rate, sorbitol and sucrose content, sorbitol 6-phosphate dehydrogenase, and sucrose phosphate synthase enzyme activities of leaves first increased and then decreased, with the highest values being observed in Zn treatment. Zn treatment significantly increased the C assimilation capability of leaves. Compared with other treatments, the C of self-retention (labeled leaves and labeled branches) was lowest in Zn treatment (61.2%) and the output of C photoassimilates was highest in Zn treatment (38.8%). C absorption of apple fruit showed a trend of Zn > Zn > Zn > Zn > CK. In summary, foliar zinc application under appropriate concentration (0.3% ZnSO·HO) enhanced photosynthesis, increased the assimilation capability of leaves, and promoted the directional transportation of photosynthate to fruit.
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http://dx.doi.org/10.13287/j.1001-9332.202106.021DOI Listing
June 2021

Inhibition of IL-17 prevents the progression of traumatic heterotopic ossification.

J Cell Mol Med 2021 Jun 29. Epub 2021 Jun 29.

Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China.

Traumatic heterotopic ossification (HO) is the abnormal formation of bone in soft tissues as a consequence of injury. However, the pathological mechanisms leading to traumatic HO remain unknown. Here, we report that aberrant expression of IL-17 promotes traumatic HO formation by activating β-catenin signalling in mouse model. We found that elevated IL-17 and β-catenin levels are correlated with a high degree of HO formation in specimens from patients and HO animals. We also show that IL-17 initiates and promotes HO progression in mice. Local injection of an IL-17 neutralizing antibody attenuates ectopic bone formation in a traumatic mouse model. IL-17 enhances the osteoblastic differentiation of mesenchymal stem cells (MSCs) by activating β-catenin signalling. Moreover, inhibition of IL-17R or β-catenin signalling by neutralizing antibodies or drugs prevents the osteogenic differentiation of isolated MSCs and decreases HO formation in mouse models. Together, our study identifies a novel role for active IL-17 as the inducer and promoter of ectopic bone formation and suggests that IL-17 inhibition might be a potential therapeutic target in traumatic HO.
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http://dx.doi.org/10.1111/jcmm.16617DOI Listing
June 2021

Tanshinone IIA alleviates acute ethanol-induced myocardial apoptosis mainly through inhibiting the expression of PDCD4 and activating the PI3K/Akt pathway.

Phytother Res 2021 Jun 24. Epub 2021 Jun 24.

Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang, China.

Myocardial apoptosis contributes to acute ethanol-induced cardiac injury. Improving immoderate apoptosis has become the potential therapeutic strategy for acute ethanol-induced heart damage. Previous studies reported that Tanshinone IIA (Tan IIA), a key ingredient extracted from Salvia miltiorrhiza Bunge, performed an anti-apoptotic role against acute ethanol-related cell damage. In this study, we investigated whether Tan IIA protected the acute ethanol-induced cardiac damage in vivo and in vitro. C57BL/6 mice were treated with acute ethanol and then treated with Tan IIA. The results showed that Tan IIA significantly improved heart function and blocked myocardial apoptosis. Acute ethanol exposure induced H9C2 cells apoptosis. Treatment with Tan IIA abrogated acute ethanol-induced H9C2 cells apoptosis. Mechanistically, Tan IIA inhibited apoptosis by downregulating the programmed cell death protein 4 (PDCD4) expression and activating the phosphoinositide 3-kinase (PI3K)/Akt pathway. Furthermore, PDCD4 overexpression abrogated Tan IIA-mediated anti-apoptotic role and activation on the PI3K/Akt pathway. Interestingly, the PI3K inhibitor (LY294002) application significantly attenuated the main protective effects of Tan IIA. In conclusion, Tan IIA improves acute ethanol-induced myocardial apoptosis mainly through regulating the PDCD4 expression and activating the PI3K/Akt signaling pathway. We provide evidence that Tan IIA is a new treatment approach for acute ethanol-induced heart damage.
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http://dx.doi.org/10.1002/ptr.7102DOI Listing
June 2021

Epigenetic Mechanism of Enrichment of A549 Lung Cancer Stem Cells with 5-Fu.

Onco Targets Ther 2021 18;14:3783-3794. Epub 2021 Jun 18.

Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, 110001, People's Republic of China.

Background: The influence of 5-fluorouracil (5-Fu) and cisplatin (CDDP) on the A549 and NCI-H226 cells was studied, and the epigenetic mechanism of enrichment of A549 lung cancer stem cells with 5-Fu was explored.

Materials And Methods: The cell proliferation of both A549 and NCI-H226 was detected by BrdU assay, and apoptosis condition was measured by flow cytometric analysis. The expressions of OCT3/4 and Nanog in cells treated with 5-Fu or CDDP were measured by immunofluorescence, Western blot and qPCR. qPCR was also performed to determine the relative expression of methyltransferase genes and miRNA. Sequencing after bisulfite treatment (BSP) was employed to detect the methylation of OCT3/4 promoter in A549 cells. And ChIP was conducted to detect the expression of H3K9Me3 and H3K9Ace.

Results: Both 5-Fu and CDDP result in the apoptosis of A549 and NCI-H226 cells and improve the expressions of has-miR-134 and has-miR-296. However, 5-Fu enhances the expression of OCT3/4 in A549 cells, and the change of methyltransferase genes and BSP results suggested some genetic differences between CDDP and 5-Fu treatment in A549 cells. ChIP assay showed that the expression of H3K9Me3 significantly decreased and H3K9Ace significantly increased in A549 cells.

Conclusion: The enrichment effect of CDDP on A549 and NCI-H226 carcinoma stem cells is inconsistent with the enrichment effect of 5-Fu. The enrichment of A549 lung cancer stem cells with 5-Fu might be related to the methylation of OCT3/4 promoter and the expression of H3K9Me3 and H3K9Ace.
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http://dx.doi.org/10.2147/OTT.S233129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218937PMC
June 2021

Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos.

Mol Hum Reprod 2021 Jun 21. Epub 2021 Jun 21.

Embryology, Anatomy and Physiology; Department Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands.

Mammalian oocytes and embryos rely exclusively on maternal mRNAs to accomplish early developmental processes. Since oocytes and early embryos are transcriptionally silent after meiotic resumption, most of the synthesised maternal mRNA does not undergo immediate translation but is instead stored in the oocyte. Quantitative RT-PCR is commonly used to quantify mRNA levels, and correct quantification relies on reverse transcription and the choice of reference genes. Different methods for reverse transcription may affect gene expression determination in oocytes. In this study, we examined the suitability of either random or oligo(dT) primers for reverse transcription to be used for quantitative RT-PCR. We further looked for changes in poly(A) length of the maternal mRNAs during oocyte maturation. Our data indicate that depending on the method of reverse transcription, the optimal combination of reference genes for normalisation differed. Surprisingly, we observed a shortening of the poly(A) tail lengths of maternal mRNA as oocytes progressed from GV to MII. Overall, our findings suggest dynamic maternal regulation of mRNA structure and gene expression during oocyte maturation and early embryo development.
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http://dx.doi.org/10.1093/molehr/gaab040DOI Listing
June 2021

Surgical outcomes in acute dacryocystitis patients undergoing endonasal endoscopic dacryocystorhinostomy with or without silicone tube intubation.

Int J Ophthalmol 2021 18;14(6):844-848. Epub 2021 Jun 18.

Department of Orbital and Oculoplastic Surgery, Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China.

Aim: To establish the necessity of silicone tube intubation in acute dacryocystitis (AD) patients undergoing endonasal endoscopic dacryocystorhinostomy (En-DCR).

Methods: Patients presenting with unilateral AD were randomly assigned to two treatment groups. En-DCR procedures were performed following lacrimal abscess formation, with the operation being performed with silicone intubation for patients in group B but not group A. Functional success was defined by an absence of additional AD episodes, no epiphora, and ostium patency as established via endoscopic evaluation or fluorescein irrigation. Operative success rates and demographic variables were compared between treatment groups.

Results: In total, 66 patients were analyzed in the present study (33 per group), with complete postoperative data having been successfully collected from 27 and 22 patients in group A and group B, respectively. All patients exhibited complete resolution of acute inflammation. Upon follow-up, granulation tissue was detected around the ostium at higher rates in group B (9/22, 40.9%) relative to group A (4/27, 14.8%). At the 12-month follow-up time point, patients in group A exhibited higher success rates (25/27, 92.6%) relative to patients in group B (20/22, 90.9%), but this difference was not significant. Cases of lacrimal passage reconstruction failure in both groups were attributed to excessive fibrous and/or granulation tissue formation proximal to the intranasal ostium.

Conclusion: Given that these two operative approaches are associated with similar rates of operative success and in light of differences in granulation tissue formation, cost, and operative duration, these data do not support the routine silicone intubation of AD patients following En-DCR surgery.
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http://dx.doi.org/10.18240/ijo.2021.06.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165628PMC
June 2021

Design method for an off-axis reflective anamorphic optical system with aberration balance and constraint control.

Appl Opt 2021 Jun;60(16):4557-4566

This paper proposes a design method for an off-axis reflective anamorphic optical system (ORAOS). This method first applies vector aberration theory to establish a mathematical model to balance the aberration of an ORAOS. It then builds the error function of structural parameters and constraints through spatial ray tracing and grouping design. Next, it introduces automatic adjustment of weight factors for dynamic balance of aberrations and constraints. A particle swarm simulated annealing algorithm is used to iteratively calculate the initial structure of the ORAOS. Finally, we use an extreme ultraviolet (EUV) lithographic projection objective with off-axis six-reflective anamorphic mirrors (${\beta _x} = 1/4,{\beta _y} = 1/8$) as an example to verify the effectiveness of this method. We obtain an EUV lithographic anamorphic objective with a numerical aperture of 0.55 and a root mean square wavefront error better than $1/30\lambda$ (${\hat{\text I}}\gg\, = 13.5\;{\rm nm}$).
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http://dx.doi.org/10.1364/AO.427713DOI Listing
June 2021

Lateral epicondyle osteotomy approach for coronal shear fractures of the distal humerus: Report of three cases and review of the literature.

World J Clin Cases 2021 Jun;9(17):4318-4326

Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China.

Background: Coronal shear fractures of the distal humerus are rare injuries and are technically challenging to manage. Open reduction and internal fixation (ORIF) has become the preferred treatment because it provides anatomical reduction, stable internal fixation, and early motion, but the optimal surgical approach remains controversial.

Case Summary: We report three cases of coronal shear fractures of the distal humerus treated successfully by ORIF a novel surgical approach, in which lateral epicondyle osteotomy was performed based on the extended lateral approach. We named the novel surgical approach the lateral epicondyle osteotomy approach. All patients underwent surgical treatment and were discharged successfully. All patients had excellent functional results according to the Mayo elbow performance score. The average range of motion was 118° in flexion/extension and 172° in pronation/ supination. Only case 2 had a complication, which was implant prolapse.

Conclusion: We demonstrated that the lateral epicondyle osteotomy approach in ORIF is effective and safe for coronal shear fractures of the distal humerus.
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http://dx.doi.org/10.12998/wjcc.v9.i17.4318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173410PMC
June 2021

Immediate Endoscopic Dacryocystorhinostomy in Patients With New Onset Acute Dacryocystitis.

Laryngoscope 2021 Jun 14. Epub 2021 Jun 14.

Department of Orbital and Oculoplastic Surgery, Eye Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Objectives: To compare the results of immediate endoscopic dacryocystorhinostomy (En-DCR) and delayed En-DCR in the treatment of new-onset acute dacryocystitis (AD).

Study Design: This report describes a prospective randomized controlled interventional case series.

Methods: Between April 2009 and May 2019, 176 adults presenting at a tertiary eye care center with new-onset AD manifesting within the last 48 hours were randomized into two groups. Altogether, 160 patients (48 male, 112 female) were included in this study, with a median age of 52.8 years (range: 18-82). Patients in group A underwent urgent En-DCR, although those in group B underwent a delayed En-DCR after 2 to 5 days of systematic antibiotic treatment. Variables compared between these two groups included the time for resolution of acute external inflammation, free lacrimal passage reconstruction (LPR) success rates, and complication rates.

Results: Postoperative data were collected from 86 patients in group A and 74 patients in group B. Patients that underwent immediate En-DCR exhibited a quicker resolution of acute inflammation (P < .05). Patients that underwent delayed surgery experienced compilations of acute inflammation, with 10 ultimately developing skin fistulization and 4 exhibiting orbital cellulitis before surgery. The success rate of LPR at 12 months after surgery was higher in group A (81/86 94.2%) relative to group B (62/74 83.8%; P < .05).

Conclusions: Immediate En-DCR is associated with quicker disease resolution and a higher long-term success rate, although reducing the incidence of complications including skin fistulization and the spread of infection.

Level Of Evidence: 3 Laryngoscope, 2021 Laryngoscope, 2021.
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http://dx.doi.org/10.1002/lary.29693DOI Listing
June 2021

Prognostic Role of High Sensitivity C-Reactive Protein in Patients With Acute Myocardial Infarction.

Front Cardiovasc Med 2021 24;8:659446. Epub 2021 May 24.

Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

High sensitivity CRP (hs-CRP) has attracted intense interest in risk assessment. We aimed to explore its prognostic value in patients with acute myocardial infarction (AMI). We enrolled 4,504 consecutive AMI patients in this prospective cohort study. The associations between hs-CRP levels with the incidence of in-hospital HF was evaluated by logistic regression analysis. The association between hs-CRP levels and the cumulative incidence of HF after hospitalization were evaluated by Fine-Gray proportional sub-distribution hazards models, accounting for death without HF as competing risk. Cox proportional hazards regression models were constructed to estimate the association between hs-CRP levels and the risk of all-cause mortality. Over a median follow-up of 1 year, 1,112 (24.7%) patients developed in-hospital HF, 571 (18.9%) patients developed HF post-discharge and 262 (8.2%) patients died. In the fully adjusted model, the risk of in-hospital heart failure (HF) [95% confidence intervals (CI)] among those patients with hs-CRP values in quartile 3 (Q3) and Q4 were 1.36 (1.05-1.77) and 1.41 (1.07-1.85) times as high as the risk among patients in Q1 ( trend < 0.001). Patients with hs-CRP values in Q3 and Q4 had 1.33 (1.00-1.76) and 1.80 times (1.37-2.36) as high as the risk of HF post-discharge compared with patients in Q1 respectively ( trend < 0.001). Patients with hs-CRP values in Q3 and Q4 had 1.74 (1.08-2.82) and 2.42 times (1.52-3.87) as high as the risk of death compared with patients in Q1 respectively ( trend < 0.001). Hs-CRP was found to be associated with the incidence of in-hospital HF, HF post-discharge and all-cause mortality in patients with AMI.
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http://dx.doi.org/10.3389/fcvm.2021.659446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181755PMC
May 2021

[Metabolic regulation in constructing microbial cell factories].

Sheng Wu Gong Cheng Xue Bao 2021 May;37(5):1541-1563

CAS Key Laboratory of Microbial Physiological & Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

The regulation of the expression of genes involved in metabolic pathways, termed as metabolic regulation, is vital to construct efficient microbial cell factories. With the continuous breakthroughs in synthetic biology, the mining and artificial design of high-quality regulatory elements have substantially improved our ability to modify and regulate cellular metabolic networks and its activities. The research on metabolic regulation has also evolved from the static regulation of single genes to the intelligent and precise dynamic regulation at the systems level. This review briefly summarizes the advances of metabolic regulation technologies in the past 30 years.
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http://dx.doi.org/10.13345/j.cjb.200688DOI Listing
May 2021

Hsa-miR-372-5p regulates the NIMA related kinase 7 and IL-1β release in NK/T-cell lymphoma.

Leuk Lymphoma 2021 Jun 3:1-9. Epub 2021 Jun 3.

Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, China.

Epstein-Barr virus (EBV) infection is prevalent and associated with distinct diseases including infectious mononucleosis (IM), chronic active EBV infection (CAEBV) and NK/T-cell lymphoma (NKTL). However, the specific roles of EBV in these diseases remain unclear. Here, the whole miRNA expression datasets derived from 7 IM, 6 CAEBV, and 3 NKTL biopsies were obtained. microRNA-372-5p (Hsa-miR-372-5p) was upregulated in both CAEBV and NKTL patients. Overexpression of hsa-miR-372-5p altered the expression of over 100 proteins. In addition, hsa-miR-372-5p may target NIMA related kinase 7 to regulate NLRP3 inflammasome activation in host cell. Taken together, we reported different miRNA expression profiles in distinct EBV associated diseases, which provided novel insights to understand how host miRNAs contribute to the mechanism of EBV associated diseases. Hsa-miR-372-5p, as well as other differential expressed miRNA, might serve as potential targets in the therapy of various EBV associated diseases.
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http://dx.doi.org/10.1080/10428194.2021.1933472DOI Listing
June 2021

Lysophosphatidic Acid Accelerates Bovine In Vitro-Produced Blastocyst Formation through the Hippo/YAP Pathway.

Int J Mol Sci 2021 May 31;22(11). Epub 2021 May 31.

Embryology, Anatomy and Physiology, Department Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands.

The segregation of trophectoderm (TE) and inner cell mass in early embryos is driven primarily by the transcription factor CDX2. The signals that trigger CDX2 activation are, however, less clear. In mouse embryos, the Hippo-YAP signaling pathway is important for the activation of CDX2 expression; it is less clear whether this relationship is conserved in other mammals. Lysophosphatidic acid (LPA) has been reported to increase YAP levels by inhibiting its degradation. In this study, we cultured bovine embryos in the presence of LPA and examined changes in gene and protein expression. LPA was found to accelerate the onset of blastocyst formation on days 5 and 6, without changing the TE/inner cell mass ratio. We further observed that the expression of and was up-regulated, and YAP was overexpressed, in LPA-treated day 6 embryos. However, LPA-induced up-regulation of CDX2 expression was only evident in day 8 embryos. Overall, our data suggest that the Hippo signaling pathway is involved in the initiation of bovine blastocyst formation, but does not affect the cell lineage constitution of blastocysts.
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http://dx.doi.org/10.3390/ijms22115915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198578PMC
May 2021

Circular RNA 0102049 suppresses the progression of osteosarcoma through modulating miR-520g-3p/PLK2 axis.

Bioengineered 2021 12;12(1):2022-2032

Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou, China.

Circular RNAs (circRNAs) are a type of non-coding RNAs generated from back splicing to enhance or inhibit the progression of multiple human cancers including osteosarcoma (OS). Although circ_0102049 has been found to be highly expressed in OS cell lines, the role and specific mechanism of circ_0102049 in OS remains unclear. Here, we found that silence of circ_0102049 could significantly exacerbate the tumorigenesis of OS through sponging microRNA-520g-3p. Polo-like kinase 2 (PLK2) was predicted to be a target of miR-520g-3p, and luciferase reporter assay revealed that overexpression of miR-520g-3p dramatically suppressed the expression of PLK2, whereas miR-520g-3p inhibitor promoted the PLK2 expression. Moreover, the silence of circ_0102049 could markedly promote the proliferation, invasion, migration and cell-cycle promotion while inhibiting the apoptosis of OS cell line MG63 cells through regulating miR-520g-3p/PLK2 axis. Taken together, the present study indicated that circ_0102049 suppressed the progression of osteosarcoma via modulating miR-520g-3p/PLK2/TAp73 axis, providing a potential therapeutic target for OS.
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http://dx.doi.org/10.1080/21655979.2021.1923259DOI Listing
December 2021

Gut Microbiota Dysbiosis in Human Hypertension: A Systematic Review of Observational Studies.

Front Cardiovasc Med 2021 14;8:650227. Epub 2021 May 14.

Department of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, China.

Hypertension is one of the major risk factors to human health and human studies on association between gut microbiota and hypertension or blood pressure have received increased attention. In the present study, we aim to evaluate gut microbiota dysbiosis in human hypertension using a method of systematic review. PubMed, EMBASE, and Web of Science databases were searched until March 2021 to identify eligible articles. Additional articles were also identified by searching specific authors in this field. Inclusion criteria were observational studies based on stool samples with hypertension group and control group. Newcastle-Ottawa quality assessment scale (NOS) was used to assess the quality of the included studies. PROSPERO registration number: CRD42020212219. A total of 17 studies enrolling 9,085 participants were included. Fifteen of the enrolled studies showed good quality and two studies showed fair quality based on NOS. We found alpha diversity in hypertension decreased significantly and microbial structure can be separated compared with control groups. Gut microbiota of hypertension showed depletion of short chain fatty acids (SCFAs) producers and over-growth of some and members. Up-regulation of lipopolysaccharide biosynthesis, phosphotransferase system, ABC transporters, etc. and down-regulation of some amino acid metabolism, etc. in hypertension were reported. Fecal SCFAs levels increased and plasma SCFAs levels decreased in hypertension. Stronger microbial interactions in hypertension were seen. In conclusion, gut microbiota dysbiosis was observed in hypertension, including decreased diversity, altered microbial structure, compositional change of taxa, alterations of microbial function, nutritional and immunological factors, and microbial interactions. Poor absorption and high excretion of SCFAs may play an important role in the pathogenesis of hypertension. These findings may provide insights into etiology study and new microbial-based therapies of hypertension. PROSPERO database, identifier CRD42020212219.
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http://dx.doi.org/10.3389/fcvm.2021.650227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160125PMC
May 2021

Effect of circular RNA, mmu_circ_0000296, on neuronal apoptosis in chronic cerebral ischaemia via the miR-194-5p/Runx3/Sirt1 axis.

Cell Death Discov 2021 May 29;7(1):124. Epub 2021 May 29.

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.

Chronic cerebral ischaemia (CCI) is a common pathological disorder, which is associated with various diseases, such as cerebral arteriosclerosis and vascular dementia, resulting in neurological dysfunction. As a type of non-coding RNA, circular RNA is involved in regulating the occurrence and development of diseases, such as ischaemic brain injury. Here, we found that HT22 cells and hippocampus treated with CCI had low expression of circ_0000296, Runx3, Sirt1, but high expression of miR-194-5p. Overexpression of circ_0000296, Runx3, Sirt1, and silenced miR-194-5p significantly inhibited neuronal apoptosis induced by CCI. This study demonstrated that circ_0000296 specifically bound to miR-194-5p; miR-194-5p bound to the 3'UTR region of Runx3 mRNA; Runx3 directly bound to the promoter region of Sirt1, enhancing its transcriptional activity. Overexpression of circ_0000296 by miR-194-5p reduced the negative regulatory effect of miR-194-5p on Runx3, promoted the transcriptional effect of Runx3 on Sirt1, and inhibited neuronal apoptosis induced by CCI. mmu_circ_0000296 plays an important role in regulating neuronal apoptosis induced by CCI through miR-194-5p/Runx3/Sirt1 pathway.
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http://dx.doi.org/10.1038/s41420-021-00507-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164632PMC
May 2021

Regulation and control methods for the unattached fraction of Rn progeny in a Rn progeny chamber.

J Environ Radioact 2021 Sep 26;235-236:106653. Epub 2021 May 26.

School of Nuclear Science and Technology, University of South China, Hengyang, Hunan, 421001, China.

The unattached fraction of Rn progeny is one of the key parameters in lung dose evaluation models. To study the behavior of unattached Rn progeny and to prepare for calibration of the unattached Rn progeny monitors, a Rn progeny chamber that could stably regulate different unattached fractions of Rn progeny was established. A recirculation loop to eliminate the effects of the sampling and a loop connected to the dilution chamber to supplement the carrier aerosol were set up. Also, a theoretical model regarding the regulation of the unattached fraction of Rn progeny was established. Continuous measurements of the unattached fraction were conducted using the combination of a single-layer wire screen and a filter membrane. The experimental results showed that, under different aerosol levels, the relative standard deviation (RSD) of Pb concentration during the continuous samplings was within 10.7% and the RSD of the unattached fraction of Pb was within 9.9%. The relative deviation between the theoretical and the experimental values of Pb concentration was within 16.7% and the model calculation values of the unattached fraction of Pb were consistent with measured results.
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http://dx.doi.org/10.1016/j.jenvrad.2021.106653DOI Listing
September 2021