Publications by authors named "Bo Wei"

645 Publications

Criminal of Adverse Pregnant Outcomes: A Perspective From Thyroid Hormone Disturbance Caused by SARS-CoV-2.

Front Cell Infect Microbiol 2021 3;11:791654. Epub 2022 Jan 3.

Department of General Surgery, Xiangya Hospital Central South University, Changsha, China.

Nowadays, emerging evidence has shown adverse pregnancy outcomes, including preterm birth, preeclampsia, cesarean, and perinatal death, occurring in pregnant women after getting infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but the underlying mechanisms remain elusive. Thyroid hormone disturbance has been unveiled consistently in various studies. As commonly known, thyroid hormone is vital for promoting pregnancy and optimal fetal growth and development. Even mild thyroid dysfunction can cause adverse pregnancy outcomes. We explored and summarized possible mechanisms of thyroid hormone abnormality in pregnant women after coronavirus disease 2019 (COVID-19) infection and made a scientific thypothesis that adverse pregnancy outcomes can be the result of thyroid hormone disorder during COVID-19. In which case, we accentuate the importance of thyroid hormone surveillance for COVID-19-infected pregnant women.
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http://dx.doi.org/10.3389/fcimb.2021.791654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761741PMC
January 2022

A Rapid Cytological Screening as pre-Endoscopy Screening for Early Esophageal Squamous Cell Lesions: A Prospective Pilot Study from a Chinese Academic Center.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338211066200

Department of Gastroenterology, Zhongda Hospital, School of Medicine, Southeast University, 87 Dingjiaqiao Road, 210009, Nanjing, China.

Cytological detection of early esophageal squamous cell carcinoma (ESCC) remains challenging. Therefore, we introduced a rapid cytological screening method and evaluated its efficacy as a pre-endoscopy screening for early ESCC and precursor lesions. This method consisted of a sponge sample retrieval, automatic liquid-based cytological treatment and slides preparation, computer-assisted screening and manual diagnosis. Efficacy for detection of early ESCC and precursor lesions was evaluated. Also, diagnostic efficiency was compared with manual diagnosis. Eighty-three patients with early ESCC and precursor lesions and 2,090 asymptomatic participants with high risks of ESCC were enrolled. Whole procedure was accomplished within two working days. Abnormal cells were detected in all 83 patients, and in 272 (13.01%) subjects among 2,090 asymptomatic participants. Early ESCC, high-grade intraepithelial neoplasia, low-grade intraepithelial neoplasia and reflux esophagitis and normal endoscopic findings were detected in 8, 13, 11, 187 and 53 participants with abnormal cells, respectively. The calculated sensitivity, specificity, positive predictive value and negative predictive value for detection of early ESCC and precursor lesions were 100%, 88.34%, 11.76%, and 100%, respectively. Compared with manual diagnosis, this method was accomplished in a shorter time duration (5.4 ± 0.45 min vs 320.2 ± 132.4 min,  < 0.001), a higher diagnostic accuracy (96.7% vs74.4%,  = 0.015) and a better inter-observer agreement (93.3% vs66.7%, K = 0.286,  < 0.001). Our study provides a promising methodology as pre-endoscopy screening for early ESCC and precursor lesions.
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http://dx.doi.org/10.1177/15330338211066200DOI Listing
January 2022

Nonmetal doped carbon nitride nanosheet as photocatalyst for degradation of 4, 5-dichloroguaiacol.

Environ Res 2022 Jan 4;207:112623. Epub 2022 Jan 4.

Environment Research Institute, Shandong University, Qingdao, 266237, PR China. Electronic address:

Metal-free photocatalysts for high efficient photocatalytic degradation of pollutants have attracted growing concern in recent years. Herein, relying on density functional theory (DFT) calculations, boron and phosphorus doped CN layers were explored for the potential of utilization as photocatalysts for 4, 5-dichloroguaiacol (4, 5-DCG) removal. Our computations revealed that the adsorption energy of 4, 5-DCG on [email protected] CN layers were 26.56 kcal mol, and the ΔG of initial reactions of 4, 5-DCG with OH were also reduced onto the [email protected] CN substrates. The band gap of [email protected] CN was 2.27 eV. The obtained results showed that the doping of boron atom into CN layer narrows bandgap, and retains well catalytic performance and adsorption properties. Hence, [email protected] CN layer is a promising photocatalyst for organic pollutants removal. Possible degradation pathways of 4, 5-DCG and aquatic toxicity assessment during degradation were also carried out. Products with higher toxicity would be formed and the transformation products were still toxic to three nutrient levels of aquatic organisms (green algae, fish, and daphnia).
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http://dx.doi.org/10.1016/j.envres.2021.112623DOI Listing
January 2022

AMoPO (A = Rb, Cs) and RbMoPO: new molybdophosphates with distinct polyanionic configurations.

Dalton Trans 2022 Jan 6. Epub 2022 Jan 6.

School of Chemical Engineering and Technology, Key Laboratory of Coal Clean Conversion & Chemical Engineering Process of Xinjiang Uyghur Autonomous Region, Xinjiang University, 666 Shengli Road, Urumqi 830046, China.

The exploration of A-Mo-P-O (A = Rb, Cs) systems has allowed several new Mo(V) phosphates, RbMoPO, RbMoPO and CsMoPO, to be synthesized through the spontaneous nucleation method. Single-crystal X-ray diffraction analysis reveals that the identical stoichiometry compounds RbMoPO and CsMoPO belong to different space groups 2/ and , respectively. Both compounds consist of dissimilar 1D [Mo-O-P] chains with different repeated building units, while monovalent cations fill in spaces to form 3D structures. However, RbMoPO and CsMoPO are isostructural and crystallize in the same space group of 2/. They exhibit a 3D framework structure with 0D MoOOPO groups, which are separated by Rb/Cs atoms. Interestingly, structural relationships between the different monophosphates of the A-Mo-P-O (A = Rb, Cs) systems are presented in which distinct polyanionic configurations appear owing to the A/P ratios, as well as the size of the univalent cations. Further, detailed structural comparisons, optical properties and theoretical calculations are also discussed.
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http://dx.doi.org/10.1039/d1dt03756kDOI Listing
January 2022

Transcriptome Analysis Reveals the Anti-cancerous Mechanism of Licochalcone A on Human Hepatoma Cell HepG2.

Front Nutr 2021 20;8:807574. Epub 2021 Dec 20.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Hepatocellular carcinoma is a malignancy with a low survival rate globally, and there is imperative to unearth novel natural phytochemicals as effective therapeutic strategies. Licochalcone A is a chalcone from that displayed various pharmacological efficacy. A globally transcriptome analysis was carried out to reveal the gene expression profiling to explore Licochalcone A's function as an anti-cancer phytochemical on HepG2 cells and investigate its potential mechanisms. Altogether, 6,061 dysregulated genes were detected (3,414 up-regulated and 2,647 down-regulated). SP1 was expected as the transcription factor that regulates the functions of most screened genes. GO and KEGG analysis was conducted, and the MAPK signaling pathway and the FoxO signaling pathway were two critical signal pathways. Protein-protein interaction (PPI) network analysis based on STRING platform to discover the hub genes (MAPK1, ATF4, BDNF, CASP3, etc.) in the MAPK signaling pathway and (AKT3, GADD45A, IL6, CDK2, CDKN1A, etc.) the FoxO signaling pathway. The protein level of essential genes that participated in significant pathways was consistent with the transcriptome data. This study will provide an inclusive understanding of the potential anti-cancer mechanism of Licochalcone A on hepatocellular, signifying Licochalcone A as a promising candidate for cancer therapy.
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http://dx.doi.org/10.3389/fnut.2021.807574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720858PMC
December 2021

Unravelling the effects of complexation of transition metal ions on the hydroxylation of catechol over the whole pH region.

J Environ Sci (China) 2022 May 27;115:392-402. Epub 2021 Aug 27.

Environment Research Institute, Shandong University, Qingdao 266237, China.

Catechol pollutants (CATPs) serving as chelating agents could coordinate with many metal ions to form various CATPs-metal complexes. Little information is available on the effects of complexation of metal ions on CATPs degradation. This work presents a systematical study of •OH-mediated degradation of catechol and catechol-metal complexes over the whole pH range in advanced oxidation processes (AOPs). Results show that the pH-dependent complexation of metal ions (Zn, Cu, Ti and Fe) promotes the deprotonation of catechol under neutral and even acidic conditions. The radical adduct formation (RAF) reactions are both thermodynamically and kinetically favorable for all dissociation and complexation species, and OH/O group-containing C positions are more vulnerable to •OH attack. The kinetic results show that the complexation of the four metal ions offers a wide pH range of effectiveness for catechol degradation. At pH 7, the apparent rate constant (k) values for different systems follow the order of catechol+Ti ≈ catechol+Zn > catechol+Cu > catechol+Fe > catechol. The mechanistic and kinetic results would greatly improve our understanding of the degradation of CATPs-metal and other organics-metal complexes in AOPs. The toxicity assessment indicates that the •OH-based AOPs have the ability for decreasing the toxicity and increasing the biodegradability during the processes of catechol degradation.
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http://dx.doi.org/10.1016/j.jes.2021.08.011DOI Listing
May 2022

It Is Advisable to Control the Duration of Hypothermia Circulatory Arrest During Aortic Dissection Surgery: Single-Center Experience.

Front Cardiovasc Med 2021 10;8:773268. Epub 2021 Dec 10.

Department of Vascular Surgery, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The duration of hypothermic circulatory arrest (HCA) is one of the important factors affecting the prognosis of arch surgery, which is still controversial. The purpose of this study was to investigate the effect of HCA duration on early prognosis in type A aortic dissection (TAAD) patients who underwent arch surgery in our center. All consecutive patients who underwent surgical treatment for TAAD in Fuwai Hospital from January 2013 to December 2018 were included in this study and divided into four quartile groups based on HCA time. Baseline characteristics, perioperative indicators, and early mortality were statistically analyzed by propensity score matching (PSM) and restricted cubic spline (RCS) method. Perioperative adverse events were confirmed according to the American STS database and Penn classification. About 1,018 consecutive patients (mean age 49.11 ± 1.4 years, male 74.7%) with TAAD treated surgically were eventually included in this study. After PSM, with the prolongation of HCA time, the surgical mortality rates of group [2,15], (15,18], (18,22], and (22,73] were 4.1, 6.6, 7.8, and 10.9% with = 0.041, respectively. As shown in RCS, the mortality rate increased sharply after the HCA time exceeded 22 min. And from the subgroup analysis, the HCA time of 22 min or less was associated with better clinical outcomes (OR 2.09, 95%CI 1.25-3.45, = 0.004). The early mortality increases significantly with the duration of HCA time when arch surgery was performed. And multiple systems throughout the body can be adversely affected.
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http://dx.doi.org/10.3389/fcvm.2021.773268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8702722PMC
December 2021

Integral Analyses of Competing Endogenous RNA Mechanisms and DNA Methylation Reveal Regulatory Mechanisms in Osteosarcoma.

Front Cell Dev Biol 2021 9;9:763347. Epub 2021 Dec 9.

Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Osteosarcoma (OS) is the most common primary malignant bone tumour in children and adolescents, with rapid growth, frequent metastasis, and a poor prognosis, but its pathogenesis has not been fully elucidated. Exploring the pathogenesis of OS is of great significance for improving diagnoses and finding new therapeutic targets. Differentially expressed circRNAs (DECs), miRNAs (DEMs), methylated DNA sites (DMSs), and mRNAs (DEGs) were identified between OS and control cell lines. GSEA of DEGs and functional enrichment analysis of methylated DEGs were carried out to further identify potential biological processes. Online tools were used to predict the miRNA binding sites of DECs and the mRNA binding sites of DEMs, and then construct a circRNA-miRNA-mRNA network. Next, an analysis of the interaction between methylated DEGs was performed with a protein-protein interaction (PPI) network, and hub gene identification and survival analysis were carried out. The expression pattern of circRNA-miRNA-mRNA was validated by real-time PCR. GSEA and functional enrichment analysis indicated that DEGs and methylated DEGs are involved in important biological processes in cancer. Hsa_circ_0001753/has_miR_760/CD74 network was constructed and validated in cell lines. Low expression levels of CD74 are associated with poor overall survival times and show good diagnostic ability. Methylated DEGs may be involved in the development of OS, and the hsa_circ_0001753/has_miR_760/CD74 network may serve as a target for the early diagnosis of and targeted therapy for OS.
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http://dx.doi.org/10.3389/fcell.2021.763347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696003PMC
December 2021

Glucose starvation suppresses gastric cancer through targeting miR-216a-5p/Farnesyl-Diphosphate Farnesyltransferase 1 axis.

Cancer Cell Int 2021 Dec 25;21(1):704. Epub 2021 Dec 25.

Medical School of Chinese PLA, Beijing, China.

Background: Fasting mimic diet is an effect approach for gastric cancer (GC) treatment. Exploring mechanisms of glucose deprivation-mediated GC suppression is required to develop novel therapeutic regimens. Farnesyltransferase 1 (FDFT1), as a novel target in basic research, has been reported to regulate malignant progression in some types of cancer. However, biological functions of FDFT1 in GC are still unclear. This study focused on biological functions of FDFT1 in GC and the association between glucose starvation (GS) and FDFT1.

Methods: The data derived from the Kaplan-Meier Plotter database were collected to identify the relationship between survival time and FDFT1 expression levels of GC patients. Bioinformatic analysis was performed to explore the biological functions of FDFT1. The expression levels of targeted genes and microRNAs (miRNAs) were detected with immunohistochemistry, quantitative real-time PCR and western blot. Malignant behaviors were measured using cell counting, cell counting kit-8, 5-ethynyl-2-deoxyuridine, wound healing, invasion transwell assays in vitro and constructions of subcutaneous and lung-metastatic tumors in vivo. The glycolysis of GC cells was determined by a series of metabolites, including lactate acid, pyruvic acid, ATP production, rates of glucose uptake, extracellular acidification rate and oxygen consumption rate.

Results: FDFT1 was downregulated in GC and negatively correlated with pathological T stage, pathological TNM stage and cancer differentiation. High expression of FDFT1 also indicated better prognosis of GC patients. FDFT1 upregulation attenuated proliferation, migration and invasion of GC. miR-216a-5p was identified as a critical suppressor of FDFT1 expression and miR-216a-5p/FDFT1 axis regulated malignant behaviors and glycolysis of GC cells. GS suppressed malignant behaviors of GC by targeting miR-216a-5p/FDFT1 axis both in vitro and in vivo.

Conclusion: This study illustrated novel mechanisms by which GS effectively suppresses GC. FDFT1 may become a potential prognostic indicator and novel target of GC therapy.
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http://dx.doi.org/10.1186/s12935-021-02416-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710003PMC
December 2021

Pure voltage-driven spintronic neuron based on stochastic magnetization switching behaviour.

Nanotechnology 2022 Jan 18;33(15). Epub 2022 Jan 18.

Fundamentals Department, Air Force Engineering University, Xi'an 710051, People's Republic of China.

Voltage-driven stochastic magnetization switching in a nanomagnet has attracted more attention recently with its superiority in achieving energy-efficient artificial neuron. Here, a novel pure voltage-driven scheme with ∼27.66 aJ energy dissipation is proposed, which could rotate magnetization vector randomly using only a pair of electrodes covered on the multiferroic nanomagnet. Results show that the probability of 180° magnetization switching is examined as a sigmoid-like function of the voltage pulse width and magnitude, which can be utilized as the activation function of designed neuron. Considering the size errors of designed neuron in fabrication, it's found that reasonable thickness and width variations cause little effect on recognition accuracy for MNIST hand-written dataset. In other words, the designed pure voltage-driven spintronic neuron could tolerate size errors. These results open a new way toward the realization of artificial neural network with low power consumption and high reliability.
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http://dx.doi.org/10.1088/1361-6528/ac4662DOI Listing
January 2022

Enhanced Anti-Atherosclerotic Efficacy of pH-Responsively Releasable Ganglioside GM3 Delivered by Reconstituted High-Density Lipoprotein.

Int J Mol Sci 2021 Dec 20;22(24). Epub 2021 Dec 20.

College of Life Sciences, Nanchang University, 999 Xuefu Ave, Honggutan District, Nanchang 330031, China.

Recently, the atheroprotective role of endogenous GM3 and an atherogenesis-inhibiting effect of exogenous GM3 suggested a possibility of exogenous GM3 being recruited as an anti-atherosclerotic drug. This study seeks to endow exogenous GM3 with atherosclerotic targetability via reconstituted high-density lipoprotein (rHDL), an atherosclerotic targeting drug nanocarrier. Unloaded rHDL, rHDL loaded with exogenous GM3 at a low concentration (GM3-rHDL), and rHDL carrying GM3 at a relatively high concentration (GM3-rHDL) were prepared and characterized. The inhibitory effect of GM3-rHDL on lipid deposition in macrophages was confirmed, and GM3-rHDL did not affect the survival of red blood cells. In vivo experiments using ApoE mice fed a high fat diet further confirmed the anti-atherosclerotic efficacy of exogenous GM3 and demonstrated that GM3 packed in HDL nanoparticles (GM3-rHDL) has an enhanced anti-atherosclerotic efficacy and a reduced effective dose of GM3. Then, the macrophage- and atherosclerotic plaque-targeting abilities of GM3-rHD, most likely via the interaction of ApoA-I on GM3-rHDL with its receptors (e.g., SR-B1) on cells, were certified via a microsphere-based method and an aortic fragment-based method, respectively. Moreover, we found that solution acidification enhanced GM3 release from GM3-rHDL nanoparticles, implying the pH-responsive GM3 release when GM3-rHDL enters the acidic atherosclerotic plaques from the neutral blood. The rHDL-mediated atherosclerotic targetability and pH-responsive GM3 release of GM3-rHDL enhanced the anti-atherosclerotic efficacy of exogenous GM3. The development of the GM3-rHDL nanoparticle may help with the application of exogenous GM3 as a clinical drug. Moreover, the data imply that the GM3-rHDL nanoparticle has the potential of being recruited as a drug nanocarrier with atherosclerotic targetability and enhanced anti-atherosclerotic efficacy.
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http://dx.doi.org/10.3390/ijms222413624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704253PMC
December 2021

Stiffness is associated with hepatic stellate cell heterogeneity during liver fibrosis.

Am J Physiol Gastrointest Liver Physiol 2022 Feb 23;322(2):G234-G246. Epub 2021 Dec 23.

Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota.

The fibrogenic wound-healing response in liver increases stiffness. Stiffness mechanotransduction, in turn, amplifies fibrogenesis. Here, we aimed to understand the distribution of stiffness in fibrotic liver, how it impacts hepatic stellate cell (HSC) heterogeneity, and identify mechanisms by which stiffness amplifies fibrogenic responses. Magnetic resonance elastography and atomic force microscopy demonstrated a heterogeneous distribution of liver stiffness at macroscopic and microscopic levels, respectively, in a carbon tetrachloride (CCl) mouse model of liver fibrosis as compared with controls. High stiffness was mainly attributed to extracellular matrix dense areas. To identify a stiffness-sensitive HSC subpopulation, we performed single-cell RNA sequencing (scRNA-seq) on primary HSCs derived from healthy versus CCl-treated mice. A subcluster of HSCs was matrix-associated with the most upregulated pathway in this subpopulation being focal adhesion signaling, including a specific protein termed four and a half LIM domains protein 2 (FHL2). In vitro, FHL2 expression was increased in primary human HSCs cultured on stiff matrix as compared with HSCs on soft matrix. Moreover, FHL2 knockdown inhibited fibronectin and collagen 1 expression, whereas its overexpression promoted matrix production. In summary, we demonstrate stiffness heterogeneity at the whole organ, lobular, and cellular level, which drives an amplification loop of fibrogenesis through specific focal adhesion molecular pathways. The fibrogenic wound-healing response in liver increases stiffness. Here, macro and microheterogeneity of liver stiffness correlate with HSC heterogeneity in a hepatic fibrosis mouse model. Fibrogenic HSCs localized in stiff collagen-high areas upregulate the expression of focal adhesion molecule FHL2, which, in turn, promotes extracellular matrix protein expression. These results demonstrate that stiffness heterogeneity at the whole organ, lobular, and cellular level drives an amplification loop of fibrogenesis through specific focal adhesion molecular pathways.
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http://dx.doi.org/10.1152/ajpgi.00254.2021DOI Listing
February 2022

Discovery of Potent and Selective 2-(Benzylthio)pyrimidine-based DCN1-UBC12 Inhibitors for Anticardiac Fibrotic Effects.

J Med Chem 2022 Jan 23;65(1):163-190. Epub 2021 Dec 23.

State Key Laboratory of Esophageal Cancer Prevention and Treatment; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China; School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P. R. China.

DCN1, a co-E3 ligase, interacts with UBC12 and activates cullin-RING ligases (CRLs) by catalyzing cullin neddylation. Although DCN1 has been recognized as an important therapeutic target for human diseases, its role in the cardiovascular area remains unknown. Here, we first found that DCN1 was upregulated in isolated cardiac fibroblasts (CFs) treated by angiotensin (Ang) II and in mouse hearts after pressure overload. Then, structure-based optimizations for DCN1-UBC12 inhibitors were performed based on our previous work, yielding compound . specifically targeted DCN1 at molecular and cellular levels as shown by molecular modeling studies, HTRF, cellular thermal shift and co-immunoprecipitation assays. Importantly, effectively reversed Ang II-induced cardiac fibroblast activation, which was associated with the inhibition of cullin 3 neddylation. Our findings indicate a potentially unrecognized role of DCN1 inhibition for anticardiac fibrotic effects. may be used as a lead compound for further development.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01207DOI Listing
January 2022

Shigella handcuffs caspases.

Nat Microbiol 2022 Jan;7(1):20-21

Department of Immunology and Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA.

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http://dx.doi.org/10.1038/s41564-021-01033-4DOI Listing
January 2022

Deep-E: A Fully-Dense Neural Network for Improving the Elevation Resolution in Linear-array-based Photoacoustic Tomography.

IEEE Trans Med Imaging 2021 Dec 20;PP. Epub 2021 Dec 20.

Linear-array-based photoacoustic tomography has shown broad applications in biomedical research and preclinical imaging. However, the elevational resolution of a linear array is fundamentally limited due to the weak cylindrical focus of the transducer element. While several methods have been proposed to address this issue, they have all handled the problem in a less time-efficient way. In this work, we propose to improve the elevational resolution of a linear array through Deep-E, a fully dense neural network based on U-net. Deep-E exhibits high computational efficiency by converting the three-dimensional problem into a two-dimension problem: it focused on training a model to enhance the resolution along elevational direction by only using the 2D slices in the axial and elevational plane and thereby reducing the computational burden in simulation and training. We demonstrated the efficacy of Deep-E using various datasets, including simulation, phantom, and human subject results. We found that Deep-E could improve elevational resolution by at least four times and recover the object's true size. We envision that Deep-E will have a significant impact in linear-array-based photoacoustic imaging studies by providing high-speed and high-resolution image enhancement.
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http://dx.doi.org/10.1109/TMI.2021.3137060DOI Listing
December 2021

A novel EZH2/NXPH4/CDKN2A axis is involved in regulating the proliferation and migration of non-small cell lung cancer cells.

Biosci Biotechnol Biochem 2021 Dec 17. Epub 2021 Dec 17.

Department of respiratory medicine, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.

NXPH4 is discovered to be a neuropeptide-like glycoprotein, belonging to the Neurexophilins (Nxphs) family. NXPH4 shares a similar domain structure with NXPH1, which, however, is poorly understood in terms of its function. Bioinformatics analysis and experimental verification in this study confirmed the abnormal high expression of NXPH4 in non-small cell lung cancer (NSCLC) tissues and cells. Knockdown of NXPH4 by siRNA can inhibit the proliferation and migration of cells, resulting in significant cell cycle arrest in S1 phase. Furthermore, in NSCLC cells, NXPH4 was regulated by transcriptional activation of Enhancer of zeste homolog 2 (EZH2) in its upstream. While downstream, NXPH4 could interact with CDKN2A and downregulate its protein stability, thus participating in the cell cycle regulation through interacting with cyclinD-CDK4/6-pRB-E2F signaling pathway. To sum up, the present study reveals a regulatory pathway of EZH2/NXPH4/CDKN2A in NSCLC, providing possible reference for understanding the function of NXPH4 in tumors.
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http://dx.doi.org/10.1093/bbb/zbab217DOI Listing
December 2021

Recent Advances in Pharmacological Intervention of Osteoarthritis: A Biological Aspect.

Front Pharmacol 2021 23;12:772678. Epub 2021 Nov 23.

Affiliated Hospital of Guangdong Medical University, Guangdong Medical University, Zhanjiang, China.

Osteoarthritis (OA) is a degenerative joint disease in the musculoskeletal system with a relatively high incidence and disability rate in the elderly. It is characterized by the degradation of articular cartilage, inflammation of the synovial membrane, and abnormal structure in the periarticular and subchondral bones. Although progress has been made in uncovering the molecular mechanism, the etiology of OA is still complicated and unclear. Nevertheless, there is no treatment method that can effectively prevent or reverse the deterioration of cartilage and bone structure. In recent years, in the field of pharmacology, research focus has shifted to disease prevention and early treatment rather than disease modification in OA. Biologic agents become more and more attractive as their direct or indirect intervention effects on the initiation or development of OA. In this review, we will discuss a wide spectrum of biologic agents ranging from DNA, noncoding RNA, exosome, platelet-rich plasma (PRP), to protein. We searched for key words such as OA, DNA, gene, RNA, exosome, PRP, protein, and so on. From the pharmacological aspect, stem cell therapy is a very special technique, which is not included in this review. The literatures ranging from January 2016 to August 2021 were included and summarized. In this review, we aim to help readers have a complete and precise understanding of the current pharmacological research progress in the intervention of OA from the biological aspect and provide an indication for the future translational studies.
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http://dx.doi.org/10.3389/fphar.2021.772678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649959PMC
November 2021

Decreased Plasma Hydrogen Sulfide Level Is Associated With the Severity of Depression in Patients With Depressive Disorder.

Front Psychiatry 2021 11;12:765664. Epub 2021 Nov 11.

Biological Psychiatry Laboratory, Jiangxi Mental Hospital/Affiliated Mental Hospital of Nanchang University, Nanchang, China.

Accumulating evidence has suggested a dysfunction of synaptic plasticity in the pathophysiology of depression. Hydrogen sulfide (HS), an endogenous gasotransmitter that regulates synaptic plasticity, has been demonstrated to contribute to depressive-like behaviors in rodents. The current study investigated the relationship between plasma HS levels and the depressive symptoms in patients with depression. Forty-seven depressed patients and 51 healthy individuals were recruited in this study. The 17-item Hamilton Depression Rating Scale (HAMD-17) was used to evaluate depressive symptoms for all subjects and the reversed-phase high-performance liquid chromatography (RP-HPLC) was used to measure plasmaHS levels. We found that plasma HS levels were significantly lower in patients with depression relative to healthy individuals ( < 0.001). Compared with healthy controls (1.02 ± 0.34 μmol/L), the plasma HS level significantly decreased in patients with mild depression (0.84 ± 0.28 μmol/L), with moderate depression (0.62 ± 0.21μmol/L), and with severe depression (0.38 ± 0.18 μmol/L). Correlation analysis revealed that plasma HS levels were significantly negatively correlated with the HAMD-17 scores in patients ( = -0.484, = 0.001). Multivariate linear regression analysis showed that plasma HS was an independent contributor to the HAMD-17 score in patients ( = -0.360, = -2.550, = 0.015). Collectively, these results suggest that decreased HS is involved in the pathophysiology of depression, and plasma HS might be a potential indicator for depression severity.
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http://dx.doi.org/10.3389/fpsyt.2021.765664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631961PMC
November 2021

Identification of serum exosomal miR-98-5p, miR-183-5p, miR-323-3p and miR-19b-3p as potential biomarkers for glioblastoma patients and investigation of their mechanisms.

Curr Res Transl Med 2022 01 24;70(1):103315. Epub 2021 Nov 24.

Departments of VIP Unit, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China. Electronic address:

Background: Exosomal miRNAs have attracted increasing interest as potential biomarkers and treatment targets for cancers, however, glioblastoma (GBM)-related exosomal miRNAs remain rarely reported. The study aimed to screen crucial serum exosomal miRNAs in GBM patients and explored their possible mechanisms.

Methods: Serum exosomal miRNA profile datasets of GBM patients and normal controls were downloaded from the Gene Expression Omnibus database (GSE112462 and GSE122488). The differentially expressed miRNAs (DEMs) were identified using the limma method. Their diagnostic values were assessed by receiver operating characteristic (ROC) curve analysis. The target genes of DEMs were predicted by the miRwalk 2.0 database. Function enrichment analysis was performed using the DAVID database. The expression and prognosis of target genes were validated using TCGA sequencing data and immunohistochemistry.

Results: Seven DEMs were shared in two datasets, among which hsa-miR-183-5p and hsa-miR-98-5p as well as has-miR-323-3p or has-miR-19b-3p constituted a diagnostic signature to distinguish GBM from controls, with the area under the ROC curve nearly approximate to 1. MAPK8IP1/FAM175B, OSMR/CASP3, PTPN2 and FBXO32 may be underlying targets for hsa-miR-183-5p, hsa-miR-98-5p, has-miR-323-3p and has-miR-19b-3p, respectively. Function analysis showed all of these target genes were involved in cell proliferation and related signaling pathways [positive regulation of cell proliferation (OSMR), negative regulation of transcription from RNA polymerase II promoter (PTPN2), cell division (FAM175B), regulation of transcription, DNA-templated (MAPK8IP1), hsa05200:Pathways in cancer (CASP3) and hsa04068:FoxO signaling pathway (FBXO32)]. The protein and (or mRNA) expression levels of OSMR, CASP3, PTPN2 and FBXO32 were validated to be upregulated, while MAPK8IP1 and FAM175B were downregulated in GBM tissues. Also, OSMR, CASP3, PTPN2 and FBXO32 were associated with patients' prognosis.

Conclusion: These findings suggest these four exosomal miRNAs may represent potential diagnostic biomarkers and therapeutic targets for GBM.
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http://dx.doi.org/10.1016/j.retram.2021.103315DOI Listing
January 2022

The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides.

Carbohydr Polym 2022 Jan 24;276:118699. Epub 2021 Sep 24.

Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Center for Innovation Marine Drug Screening & Evaluation and Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China. Electronic address:

Severe acute inflammatory conditions may cause tissue damage, sepsis, and death. As a critical component of the extracellular matrix, hyaluronic acid (HA) has been reported to possess pro- and anti-inflammatory properties via Toll-like receptors (TLRs). In this study, we prepared different sizes and structures of HA oligosaccharides and derivatives and investigated the effects on inflammation in vitro and in vivo. Our results showed that HA tetra-saccharide was the minimum fragment to enhance inflammation, whereas HA disaccharide competitively blocked TLR4-dependent inflammation. The enzymatic HA disaccharide (ΔHA2) inhibited lipopolysaccharide (LPS)-induced inflammation. Based on structure-activity relationship analysis, we observed that anti-inflammatory activity depended on HAs polymerization degree, acetyl group, and configuration. In addition, we demonstrated that ΔHA2 reduced LPS-induced pro-inflammatory cytokines production in vivo. ΔHA2, a native metabolite of HA polysaccharides, may have a potential role against LPS-mediated inflammatory diseases.
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http://dx.doi.org/10.1016/j.carbpol.2021.118699DOI Listing
January 2022

Theoretical insights into the degradation of swep by hydroxyl radicals in atmosphere and water environment: Mechanisms, kinetics and toxicity.

Sci Total Environ 2021 Nov 13:151651. Epub 2021 Nov 13.

Environment Research Institute, Shandong University, Qingdao 266237, PR China. Electronic address:

As an excellent conductive herbicide, swep is widely used in weed removal. Its remaining in atmosphere and water can not only contaminate the environment but also pose a threat to human health. This work presented a systematic theoretical study of HO-mediated degradation mechanisms and kinetics of swep in atmosphere and water environment. HO-addition reaction was the dominant reaction type and the main degradation products N-(3-chloro-4-hydroxyphenyl)carbamate (P2), N-(3,4-chloro-6-hydroxyphenyl)carbamate (P3) and N-(3,4-chloro-2-hydroxyphenyl)carbamate (P11) were in good agreement with the experimental results. The total rate constants of swep with HO were determined to be 3.37 × 10 and 7.73 × 10 cm molecule s (at 298 K) in atmosphere and water environment, respectively. As an excellent adsorbent and photocatalyst, zinc oxide (ZnO) was selected to study the adsorption and catalytic degradation mechanism of swep. The adsorption configuration of (ZnO) clusters with swep was most stable when n = 6. The adsorption of (ZnO) cluster was more favorable to the H-atom abstraction reaction. The toxicities of swep and its degradation products to aquatic organisms were predicted. The degradation of swep induced by HO was beneficial to the survival of aquatic organisms. This work would provide a comprehensive theoretical basis for understanding the degradation behavior of organic pollutants.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151651DOI Listing
November 2021

GABA promotes the malignancy of human cancer cells by targeting the tyrosine phosphatase PTPN12.

iScience 2021 Nov 16;24(11):103311. Epub 2021 Oct 16.

Key Laboratory of Molecular Biophysics of MOE, International Research Center for Sensory Biology and Technology of MOST, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan 430074, China.

Neurotransmitter receptors are involved in cancer progression. Among them, the heterodimeric GABA receptor, activated by the main inhibitory neurotransmitter GABA, is composed of the transmembrane GABA and GABA subunits. The oncogenic role of the isoform GABA (GB) containing only the extracellular domain of GABA remains unclear. We revealed that GB is largely expressed in human breast cancer (BrCa) cell lines as well as in BrCa tissues where it is upregulated. Moreover, GB promoted the malignancy of BrCa cells both and . We propose that GB favors EGFR signaling by interacting with PTPN12 to disrupt the interaction between EGFR and PTPN12, and phosphorylation of Y230 and Y404 on GB is required in this process. Our data highlight that the gene through the expression of the GB isoform might play an important oncogenic role in BrCa and that GB is of interest for the treatment of some cancers.
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http://dx.doi.org/10.1016/j.isci.2021.103311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8577127PMC
November 2021

Angioplasty With Stent Implantation for Portal Venous Stenosis Caused by Abdominal Tuberculosis: A Case Report and Literature Review.

Front Med (Lausanne) 2021 28;8:778672. Epub 2021 Oct 28.

Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, China.

Abdominal tuberculosis is one of common forms of extra-pulmonary tuberculosis. However, portal vein involvement leading to portal venous stenosis and portal hypertension is a rare complication in abdominal tuberculosis. Because of the non-specific presentations and insensitive response to anti-tuberculosis therapy of the lesions involving portal vein, it continues to be both a diagnostic and treatment challenge. We have reported a 22-year-old woman presented with massive ascites and pleural effusion, which was proved to be TB infection by pleural biopsy. After standard anti-tuberculosis therapy, her systemic symptoms completely resolved while ascites worsened with serum-ascites albumin gradient >11 g/L. Contrast-enhanced computed tomography and portal venography showed severe main portal vein stenosis from compression by multiple calcified hilar lymph nodes. Finally, the patient was diagnosed with portal venous stenosis due to lymphadenopathy after abdominal tuberculosis infection. Portal venous angioplasty by balloon dilation with stent implantation was performed and continued anti-tuberculosis therapy were administrated after discharge. The ascites resolved promptly with no recurrence occurred during the six-month follow-up. Refractory ascites due to portal venous stenosis is an uncommon vascular complication of abdominal tuberculosis. Portal venous angioplasty with stent placement could be a safe and effective treatment for irreversible vascular lesions after anti-tuberculosis therapy.
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http://dx.doi.org/10.3389/fmed.2021.778672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581033PMC
October 2021

Spatiotemporal Variation in Rainfall Erosivity and Correlation with the ENSO on the Tibetan Plateau since 1971.

Int J Environ Res Public Health 2021 10 21;18(21). Epub 2021 Oct 21.

Key Laboratory of Land Surface Pattern and Simulation, Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China.

Soil erosion is a serious ecological problem in the fragile ecological environment of the Tibetan Plateau (TP). Rainfall erosivity is one of the most important factors controlling soil erosion and is associated with the El Niño southern oscillation (ENSO). However, there is a lack of studies related to the spatial distribution and temporal trends of rainfall erosivity on the TP as a whole. Additionally, the understanding of the general influence of ENSO on rainfall erosivity across the TP remains to be developed. In this study, long-term (1971-2020) daily precipitation data from 91 meteorological stations were selected to calculate rainfall erosivity. The analysis combines co-kriging interpolation, Sen's slope estimator, and the Mann-Kendall trend test to investigate the spatiotemporal patten of rainfall erosivity across the TP. The Oceanic Niño Index (ONI) and multivariate ENSO Index (MEI) were chosen as ENSO phenomenon characterization indices, and the relationship between ENSO and rainfall erosivity was explored by employing a continuous wavelet transform. The results showed that an increasing trend in annual rainfall erosivity was detected on the TP from 1971 to 2020. The seasonal and monthly rainfall erosivity was highly uneven, with the summer erosivity accounting for 60.36%. The heterogeneous spatial distribution of rainfall erosivity was observed with an increasing trend from southeast to northwest. At the regional level, rainfall erosivity in the southeastern TP was mainly featured by a slow increase, while in the northwest was more destabilizing and mostly showed no significant trend. The rainfall erosivity on the whole TP was relatively high during non-ENSO periods and relatively low during El Niño/La Niña periods. It is worth noting that rainfall erosivity in the northwest TP appears to be more serious during the La Niña event. Furthermore, there were obvious resonance cycles between the rainfall erosivity and ENSO in different regions of the plateau, but the cycles had pronounced discrepancies in the occurrence time, direction of action and intensity. These findings contribute to providing references for soil erosion control on the TP and the formulation of future soil conservation strategies.
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http://dx.doi.org/10.3390/ijerph182111054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583552PMC
October 2021

Recent Advances in the Diagnosis, Staging, Treatment, and Prognosis of Advanced Gastric Cancer: A Literature Review.

Front Med (Lausanne) 2021 26;8:744839. Epub 2021 Oct 26.

Department of General Surgery, First Medical Center of Chinese People's Liberation Army of China (PLA) General Hospital, Beijing, China.

Gastric cancer is one of the most common cause of cancer related deaths worldwide which results in malignant tumors in the digestive tract. The only radical treatment option available is surgical resection. Recently, the implementation of neoadjuvant chemotherapy resulted in 5-year survival rates of 95% for early gastric cancer. The main reason of treatment failure is that early diagnosis is minimal, with many patients presenting advanced stages. Hence, the greatest benefit of radical resection is missed. Consequently, the main therapeutic approach for advanced gastric cancer is combined surgery with neoadjuvant chemotherapy, targeted therapy, or immunotherapy. In this review, we will discuss the various treatment options for advanced gastric cancer. Clinical practice and clinical research is the most practical way of reaching new advents in terms of patients' characteristics, optimum drug choice, and better prognosis. With the recent advances in gastric cancer diagnosis, staging, treatment, and prognosis, we are evident that the improvement of survival in this patient population is just a matter of time.
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http://dx.doi.org/10.3389/fmed.2021.744839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575714PMC
October 2021

Recent advancements in peripheral nerve-specific fluorescent compounds.

Biomater Sci 2021 Nov 23;9(23):7799-7810. Epub 2021 Nov 23.

Department of General Surgery, Xiangya Hospital Central South University, Changsha 410008, Hunan, P.R. China.

Nerve injury is a common complication of surgery. Accidental nerve damage or transection can lead to severe clinical symptoms including pain, numbness, paralysis and even expiratory dyspnoea. In recent years, with the rise of the field of fluorescence-guided surgery, researchers have discovered that nerve-specific fluorescent agents can serve as nerve markers in animals and can be used to guide surgical procedures and reduce the incidence of intraoperative nerve damage. Currently, researchers have begun to focus on biochemistry, materials chemistry and other fields to produce more neuro-specific fluorescent agents with physiological relevance and they are expected to have clinical applications. This review discusses the agents with potential to be used in fluorescence-guided nerve imaging during surgery.
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http://dx.doi.org/10.1039/d1bm01256hDOI Listing
November 2021

Selenium donor restricts the intracellular growth of Mycobacterium tuberculosis through the induction of c-Jun-mediated both canonical autophagy and LC3-associated phagocytosis of alveolar macrophages.

Microb Pathog 2021 Dec 3;161(Pt B):105269. Epub 2021 Nov 3.

The Eighth Medical Center, Chinese PLA General Hospital, Beijing, 100091, China; Department of Genome Sciences, John Curtin School of Medical Research, Australian National University, Australia. Electronic address:

The relationship between selenium and Mycobacterium tuberculosis (MTB) infection has been reported previously; however, the specific mechanism is still not clear. In this study, selenium levels decreased in the serum of patients with pulmonary tuberculosis (PTB) compared with the healthy controls; they were associated with the treatment outcome of such patients. The qRT-PCR assay revealed that selenium might function through proinflammatory and autophagy pathways. The treatment with methylseleninic acid (MSeA), a selenium donor, blocked the M1 polarization of MTB-infected macrophages through the induction of both canonical autophagy and LC3-associated phagocytosis (LAP). c-Jun is vital in mediating the MSeA-triggered canonical autophagy and LAP process, thus displaying a restricting function against intracellular MTB. An in vivo study confirmed that the activity of MSeA was shown through enhancing macrophage autophagy related pathway. The results showed that selenium had a restricting function against intracellular MTB by regulating autophagy in macrophages. The findings might provide a novel direction for PTB therapy in the future.
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http://dx.doi.org/10.1016/j.micpath.2021.105269DOI Listing
December 2021

Effects of marital status on survival of medullary thyroid cancer stratified by age.

Cancer Med 2021 12 1;10(24):8829-8837. Epub 2021 Nov 1.

Department of General Surgery, Xiangya Hospital Central South University, Changsha, Hunan, P.R. China.

Purpose: Marital status has emerged as an important influence on several cancer outcomes, but its role in medullary thyroid cancer (MTC) remains unclear. This study was to explore the effects of marital status on the prognosis of MTC patients and to determine whether its effects vary by age.

Patients And Methods: We retrospectively extracted 1344 eligible patients diagnosed with MTC between 2004 and 2015 from the Surveillance, Epidemiology, and End Results (SEER) database. Based on the marital status, we divided those patients into married and unmarried groups. We compared the difference in overall survival (OS) and cancer-specific survival (CSS) between married and unmarried via the Kaplan-Meier analysis. Univariate and multivariate Cox proportional models were performed to identify the prognostic factors of OS and CSS.

Results: There were 1344 MTC eligible patients in a total of which 883 (65.7%) were married and 461 (34.3%) were unmarried. The comparison observed between married and unmarried patients was as follows: male (45.2% vs. 28.0%), age (≥52 years) (55.9% vs. 44.6%), White (86.7% vs. 78.7%), and undergo surgery (97.7% vs. 93.3%). Multivariate analysis revealed unmarried status as a risk factor independently associated with worse OS (HR: 2.15, 95% CI: 1.59-2.92) rate and CSS (HR: 1.70, 95% CI: 1.17-2.47) rate. In a further analysis stratified by age, there was no significant difference in OS and CSS between married and unmarried patients younger than 52 years. For the remaining group with 52 years old and higher, unmarried patients showed significantly higher risk of OS and CSS than married patients at all stages of the pathology except M1 stage.

Conclusion: Married patients with MTC have a better prognosis than unmarried ones. Age can affect the association between marital status and the survival of MTC, and married elders may benefit more than youngers.
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http://dx.doi.org/10.1002/cam4.4388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683521PMC
December 2021

Functional Group Characteristics and Pyrolysis/Combustion Performance of Karamay OS Based on FT-IR and TG-DTG Analyses.

ACS Omega 2021 Oct 11;6(42):27684-27696. Epub 2021 Oct 11.

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources and Key Laboratory of Coal Clean Conversion & Chemical Engineering Process (Xinjiang Uyghur Autonomous Region), College of Chemical Engineering, Xinjiang University, Urumqi, Xinjiang 830046, China.

Proximate analysis, ultimate analysis, Fourier-transform infrared spectroscopy (FT-IR), and thermogravimetry-differential thermal analysis characterization were carried out on oily sludge (OS) samples OS1-OS5, from Karamay, Xinjiang, China. The Coast-Redfern model (CRm) was used to simulate the pyrolysis and combustion kinetics of oily samples. The results showed that the peak area percentage of benzene ring trisubstitution of OS5, in the range of 700-900 cm, is close to 75%, corresponding to its high volatile content. Based on the kinetic analysis by the CRm, it is found that the fitting degree of the five samples is better when the reaction order is selected as = 2, with close to 1.00 and 2/ to 0. Among them, the and of OS5 are 17.8 × 10% min °C and 0.10899 × 10% min °C, respectively, higher than those of other samples, indicating a good combustion performance.
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http://dx.doi.org/10.1021/acsomega.1c02734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552240PMC
October 2021

Comprehensive Analysis of Prognostic Alternative Splicing Signature Reveals Recurrence Predictor for Papillary Thyroid Cancer.

Front Oncol 2021 13;11:705929. Epub 2021 Oct 13.

Department of General Surgery, Xiangya Hospital Central South University, Changsha, China.

Background: Alternative splicing (AS) plays a key role in the diversity of proteins and is closely associated with tumorigenicity. The aim of this study was to systemically analyze RNA alternative splicing (AS) and identify its prognostic value for papillary thyroid cancer (PTC).

Methods: AS percent-splice-in (PSI) data of 430 patients with PTC were downloaded from the TCGA SpliceSeq database. We successfully identified recurrence-free survival (RFS)-associated AS events through univariate Cox regression, LASSO regression and multivariate regression and then constructed different types of prognostic prediction models. Gene function enrichment analysis revealed the relevant signaling pathways involved in RFS-related AS events. Simultaneously, a regulatory network diagram of AS and splicing factors (SFs) was established.

Results: We identified 1397 RFS-related AS events which could be used as the potential prognostic biomarkers for PTC. Based on these RFS-related AS events, we constructed a ten-AS event prognostic prediction signature that could distinguish high-and low-risk patients and was highly capable of predicting PTC patient prognosis. ROC curve analysis revealed the excellent predictive ability of the ten-AS events model, with an area under the curve (AUC) value of 0.889; the highest prediction intensity for one-year RFS was 0.923, indicating that the model could be used as a prognostic biomarker for PTC. In addition, the nomogram constructed by the risk score of the ten-AS model also showed high predictive efficiency for the prognosis of PTC patients. Finally, the constructed SF-AS network diagram revealed the regulatory role of SFs in PTC.

Conclusion: Through the limited analysis, AS events could be regarded as reliable prognostic biomarkers for PTC. The splicing correlation network also provided new insight into the potential molecular mechanisms of PTC.
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http://dx.doi.org/10.3389/fonc.2021.705929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548661PMC
October 2021
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