Publications by authors named "Bo Tang"

1,222 Publications

  • Page 1 of 1

Langerhans cell histiocytosis of the rib in an adult: A case report and review of the literature.

Authors:
Shengliang Zhao Chao Luo Bo Tang Liang Chen Mingzhang Xiang Jigang Dai Hua Li

Int J Surg Case Rep 2022 Jul 21;97:107432. Epub 2022 Jul 21.

Department of Thoracic Surgery, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing 400037, China. Electronic address:

Introduction And Importance: Langerhans cell histiocytosis (LCH) is a rare neoplastic hyperplasia with an unknown etiology. It is clinically rare for patients with solitary rib lesion and pathological fracture. In this article, we report a case of LCH in solitary involvement of rib and provide a review of the available literature.

Case Presentation: A 24-year-old female patient complained of right chest and back pain for 10 days. CT showed a fracture in the right 6th rib. Findings on X-ray, and CT were suggestive of homogeneous osteolytic lesion of the right 6th rib. The rib tumor was then resected and the surrounding muscles and soft tissues were accordingly resected.

Clinical Discussion: The patient was diagnosed with pathological rib fracture, and the patient was pathologically diagnosed with LCH. After surgery, no local recurrence or distant metastasis was reported during the one-year follow-up.

Conclusions: Most of the solitary tumorous lesions in rib in adults call for various differential diagnoses. Although single-site, single-system LCH of the rib is one of the rarest causes of bone tumor in adults, it can be treated successfully with surgical intervention. LCH should be considered in the diagnosis of an adult patient with a rib mass.
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http://dx.doi.org/10.1016/j.ijscr.2022.107432DOI Listing
July 2022

Exploring Idiopathic Pulmonary Fibrosis Biomarker by Simultaneous Two-Photon Fluorescence Imaging of Cysteine and Peroxynitrite.

Authors:
Hui Wang Junwei Nie Ping Li Xiaoting Zhang Yu Wang Wei Zhang Wen Zhang Bo Tang

Anal Chem 2022 Aug 4. Epub 2022 Aug 4.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

Idiopathic pulmonary fibrosis (IPF) has been characterized as a chronic inflammatory disease that leads to irreversible damage to pulmonary function. However, there is no specific IPF biomarker that can be used to distinguish IPF and not pneumonia. Endoplasmic reticulum (ER) stress is prominent in IPF. To search for a specific biomarker of IPF, we developed two ER-targeting two-photon (TP) fluorescent probes, TP-ONOO and TP-Cys, for peroxynitrite (ONOO) and cysteine (Cys) imaging, respectively. A significant increase in Cys levels in the lungs was discovered only in mice with IPF, which implied that Cys might be an IPF biomarker candidate. Furthermore, we uncovered the mechanism of glutathione (GSH) deficiency in IPF, which was not due to Cys shortage but instead was attributable to impaired glutamate cysteine ligase and glutathione synthetase activities ONOO-induced post-transcriptional modification. This work has potential to provide a new method for IPF early diagnosis and drug efficacy evaluation.
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http://dx.doi.org/10.1021/acs.analchem.2c01866DOI Listing
August 2022

Oriented interlayered charge transfer in NiCoFe layered double hydroxide/MoO stacked heterostructure promoting the oxygen-evolving behavior.

Authors:
Changgang Dong Min Guo Wen Gao Pin Hao Fengcai Lei Junfeng Xie Bo Tang

J Colloid Interface Sci 2022 Jul 21;627:891-899. Epub 2022 Jul 21.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes (Ministry of Education), Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, Shandong 250014, PR China.

Oxygen evolution reaction (OER), the rate-limiting half reaction of water electrolysis, plays as a crucial role in improving the overall efficiency of the coupled hydrogen evolution. To date, earth-abundant OER catalysts have been extensively studied, while unfortunately their catalytic activity and operational stability still need to be further optimized. In this work, we fabricated a highly efficient OER catalyst based on two-dimensional (2D) NiCoFe layered double hydroxide (LDH)/MoO stacked heterostructure with enriched active sites and optimal electronic structure via an electrostatic-driven self-assembly process. The rough surface of the 2D heterostructure could offer abundant reactive sites for the pre-oxidation reaction, thereby leading to fast generation of the high-valence active species for OER, and the multi-metal synergy and oriented interlayered charge transfer could further enhance the intrinsic OER activity, finally resulting in enhanced OER performance with low overpotential, large current density, high intrinsic activity and excellent operational stability.
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http://dx.doi.org/10.1016/j.jcis.2022.07.114DOI Listing
July 2022

Learning Curve of Robotic-Assisted Total Mesorectal Excision for Rectal Cancer.

Authors:
Bo Tang Tao Li Gengmei Gao Jun Shi Taiyuan Li

Front Oncol 2022 11;12:931426. Epub 2022 Jul 11.

Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Although some studies have assessed the learning curve of robotic-assisted total mesorectal excision for rectal cancer, most studies included limited sample sizes, no study used postoperative complications as an independent variable to analyze the learning curve of robotic rectal surgery, and no study evaluated the influence of the learning curve on long-term oncologic outcomes.

Methods: Clinical data on consecutive patients who underwent robotic-assisted total mesorectal excision for rectal cancer by a single surgeon between January 2015 and December 2018 at the First Affiliated Hospital of Nanchang University were retrospectively collected. The cumulative sum (CUSUM) and risk-adjusted cumulative sum (RA-CUSUM) were used to visualize the learning curve of operation time and postoperative complications (CD ≥ grade II). Comparisons of clinical outcomes at different learning phases analyzed by RA-CUSUM were performed after propensity score matching.

Results: A total of 389 consecutive patients were included in the analysis. The numbers of patients needed to overcome the learning curves of operation time and postoperative complications of robotic-assisted laparoscopic surgery for rectal cancer were 34 and 36, respectively. The learning process was divided into two phases based on RA-CUSUM: the learning phase (1st-36th cases) and the mastery phase (37th-389th cases). Before matching, the mastery phase had more patients with older age, lower tumor location, and neoadjuvant therapy. After matching, the two phases exhibited similar characteristics. The operation time, intraoperative blood loss, postoperative hospital stay, and postoperative complications in the mastery phase were reduced compared with the learning phase, with a median follow-up of 35 months, and the long-term oncologic outcomes were not significantly different between the two phases.

Conclusions: An experienced laparoscopic surgeon initially implements robotic-assisted total mesorectal excision for rectal cancer, surgical outcomes improved after 36 cases, and the learning curve seemingly did not have an obvious impact on long-term oncologic outcomes.
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http://dx.doi.org/10.3389/fonc.2022.931426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311896PMC
July 2022

Ratiometric Fluorescent pH Sensor Based on a Tunable Multivariate Covalent Organic Framework.

Authors:
Jie-Yu Yue Li-Ping Song Xiu-Li Ding Yu-Tong Wang Peng Yang Yu Ma Bo Tang

Anal Chem 2022 Aug 26;94(31):11062-11069. Epub 2022 Jul 26.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, P.R. China.

Ratiometric detection of pH is always significant in environmental regulation, medical diagnosis, synthetic chemistry, and beyond. The construction of practical ratiometric pH sensors with reusability is still challenging. Herein, by exploiting a multivariate strategy, we first synthesized and reported a series of novel three-component covalent organic frameworks (COF-COOH, = 33, 50, and 67) through Schiff base reaction between 2-hydroxybenzene-1,3,5-tricarbaldehyde (HTA), 4,4'-diamino-3,3'-biphenyldicarboxylic acid (DBA), and 5,5'-diamino-2,2'-bipyridine (BPY) at various molar ratios ( = [DBA]/([BPY] + [DBA]) × 100 = 33, 50, and 67). COF-COOH ( = 33, 50, and 67) displayed ratiometric pH sensing performance in acidic conditions with selectivity and repeatability. By tuning the molar ratio of DBA and BPY, the fluorescent properties, linear pH responsive ranges, and p values of COF-COOH ( = 33, 50, and 67) can be regulated. Meanwhile, the two-component COF-COOH and COF-COOH did not exhibit ratiometric pH detection ability. Moreover, the constructed three ratiometric sensors can be applied to detect pH in drug solutions and carbonated drinks with satisfactory results. This work sheds new light on the design and fabrication of innovative ratiometric fluorescent sensors using COFs.
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http://dx.doi.org/10.1021/acs.analchem.2c01999DOI Listing
August 2022

Tumor microenvironment responsive nanocarriers for gene therapy.

Authors:
Yanhua Li Kun Tang Xia Zhang Wei Pan Na Li Bo Tang

Chem Commun (Camb) 2022 Aug 4;58(63):8754-8765. Epub 2022 Aug 4.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

Stimuli responsive nanocarriers are important non-viral gene carriers for gene therapy. We discuss the stimulus conditions and then highlight various stimuli responsive nanocarriers in the tumor microenvironment for cancer gene therapy. We hope that this review will inspire readers to develop more effective stimuli responsive nanocarriers for delivering genes.
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http://dx.doi.org/10.1039/d2cc02759cDOI Listing
August 2022

Strategies for sustained release of heparin: A review.

Authors:
Xuewen Yang Qiuxiang Wang Aiwen Zhang Xinyao Shao Tianqing Liu Bo Tang Guihua Fang

Carbohydr Polym 2022 Oct 30;294:119793. Epub 2022 Jun 30.

School of Pharmacy, Nantong University, 19 Qixiu Road, Nantong, Jiangsu Province, 226001, China. Electronic address:

Heparin, a sulfate-containing linear polysaccharide, has proven preclinical and clinical efficacy for a variety of disorders. Heparin, including unfractionated heparin (UFH), low-molecular-weight heparin (LMWH), and ultra-low-molecular-weight heparin (ULMWH), is administered systematically, in the form of a solution in the clinic. However, it is eliminated quickly, due to its short half-life, especially in the case of UFH and LMWH. Frequent administration is required to ensure its therapeutic efficacy, leading to poor patient compliance. Moreover, heparin is used to coat blood-contacting medical devices to avoid thrombosis through physical interaction. However, the short-term durability of heparin on the surface of the stent limits its further application. Various advanced sustained-release strategies have been used to prolong its half-life in vivo as preparation technologies have improved. Herein, we briefly introduce the pharmacological activity and mechanisms of action of heparin. In addition, the strategies for sustained release of heparin are comprehensively summarized.
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http://dx.doi.org/10.1016/j.carbpol.2022.119793DOI Listing
October 2022

Single-ion chelation strategy for synthesis of monodisperse Pd nanoparticles anchored in MOF-808 for highly efficient hydrogenation and cascade reactions.

Authors:
Ke Zhao Le-Xi Zhang Heng Xu Yi-Fei Liu Bo Tang Li-Jian Bie

Nanoscale 2022 Aug 4;14(30):10980-10991. Epub 2022 Aug 4.

School of Materials Science and Engineering, Tianjin Key Lab for Photoelectric Materials and Devices, Key Laboratory of Display Materials and Photoelectric Devices (Ministry of Education), National Demonstration Center for Experimental Function Materials Education, Tianjin University of Technology, Tianjin 300384, China.

Ultrafine Pd nanoparticles are prepared using a single-ion precursor on a MOF-808 carrier. The ligand 2,3-pyrazinedicarboxylic acid (Pza) is dispersed in porous MOF-808 grafting on formic acid sites, and thus Pd ions are chelated by Pza to form a new single-ion precursor [email protected] Then a [email protected] catalyst is prepared by direct reduction of this precursor using NaBH. Material characterization reveals the homogeneous dispersion of 3-6 nm Pd nanoparticles within the MOF-808 matrix. [email protected] exhibits excellent catalytic activity in the hydrogenation of unsaturated nitrogen-containing compounds, and other typical reactions, such as the Knoevenagel condensation, Suzuki/Heck cross-coupling, and hydrogen tandem reactions. In addition, density functional theory (DFT) calculations are carried out to elucidate the chelation of Pd ions by Pza on MOF-808 and propose mechanisms of hydrogenation reactions. This work provides an effective reduction catalyst, and more importantly, a single-ion chelation strategy for design and synthesis of metal supported catalysts.
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http://dx.doi.org/10.1039/d2nr02765hDOI Listing
August 2022

Probing Iron-Mediated Synergistic Change of Cl and HClO in Liver Cancer Cells with a Dual-Color Fluorescence Reporter.

Authors:
Nannan Fan Yongqing Zhou Lijie Wu Xin Wang Ping Li Zhenzhen Liu Wen Zhang Wei Zhang Bo Tang

Anal Chem 2022 08 20;94(30):10659-10668. Epub 2022 Jul 20.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Biomedical Science, Shandong Normal University, Jinan 250014, P. R. China.

The ambiguous molecular mechanism remains a leading cause for the high mortality rate of liver cancer. An evident iron overload has been unveiled in liver cancer cell proliferation, which is closely related to oxidative stress. However oxidative stress-regulated chloride intracellular channel protein 1 (CLIC1) obviously increases in liver cancer cells. Cl is also involved in cell proliferation, and its downstream product, HClO, can induce cell carcinoma when over-generated. However, whether iron overload could mediate the variation of intracellular Cl and HClO is still uncharted. Herein, we present a dual-responsive fluorescence reporter MQFL-NH for simultaneously visualizing the fluctuation of Cl/HClO at the same spot in living cells. Electrostatic binding to Cl effectively gave an attenuated signal with blue fluorescence, and HClO induced a sharp green fluorescence. In HL-7702 cells stimulated with iron, the blue/green dual fluorescence of MQFL-NH displayed that Cl and HClO were elevated. In contrast, they were both reduced in iron-removed SMMC-7721 cells. Further results revealed that iron overload could promote the levels of Cl and HClO by up-regulating CLIC1 and myeloperoxidase. Altogether, the work will energetically contribute to grasp the molecular mechanism in iron overload-mediated pathogenesis of liver cancer.
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http://dx.doi.org/10.1021/acs.analchem.2c00903DOI Listing
August 2022

Rosiglitazone disrupts pancreatic ductal adenocarcinoma progression by activating the tumor suppressor ESE3/EHF.

Authors:
Hongquan Wang Shuang Wu Yan Wang Bo Tang

Cancer Biol Med 2022 Jul 21. Epub 2022 Jul 21.

Department of Pancreatic Cancer, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

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http://dx.doi.org/10.20892/j.issn.2095-3941.2022.0299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334757PMC
July 2022

Analysis of Spatio-Temporal Characteristics of Urban Economic Resilience and Influencing Factors in Guangdong-Hong Kong-Macao Greater Bay Area.

Authors:
Yuanyuan Zhang Zehui Chen Bo Tang Hua Sun

Front Public Health 2022 28;10:922096. Epub 2022 Jun 28.

School of Resources and Planning, Guangzhou Xinhua University, Guangzhou, China.

Due to the changes in the domestic and international economic situation in the post-pandemic era, the economic development of the Guangdong-Hong Kong-Macao Greater Bay Area has become unstable in many aspects. The paper adopted the Pressure-State-Response (PSR) model to build a regional economic resilience evaluation system from the perspective of public health emergencies. Then, the spatial and temporal evolution of the economic resilience of the Guangdong-Hong Kong-Macao Greater Bay Area and the influencing factors were explored by using entropy weight method, GIS and gray correlation method. The conclusions show that: (1) Temporally, the economic resilience of the Guangdong-Hong Kong-Macao Greater Bay Area has generally increased from 2010 to 2021, and is divided into three main stages: rapid development, adjustment to fluctuations and stable development. (2) Spatially, the overall pattern of economic resilience in the Guangdong-Hong Kong-Macao Greater Bay Area is high in the middle and south and low in the northwest, and shows a "stochastic-equalized-polarized" pattern of transformation. (3) In terms of influencing factors, economic status and economic response are the main dimensions affecting the resilience level of the economic system in the Guangdong-Hong Kong-Macao region. The level of scientific research and innovation, medical governance, government regulation and the rationalization of the industrial system are the key factors.
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http://dx.doi.org/10.3389/fpubh.2022.922096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273716PMC
July 2022

An active tumor-targeting organic photochemotherapy agent with naproxen for enhanced cancer therapy.

Authors:
Chang Xu Jinjie Chang Yanhua Li Kaiye Wang Wei Pan Hongyu Wang Na Li Bo Tang

Chem Commun (Camb) 2022 Aug 2;58(62):8682-8685. Epub 2022 Aug 2.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014, P. R. China.

An active tumor-targeting organic photochemotherapy agent the combination of a an organic photothermal material and a naproxen prodrug was developed to precisely kill cancer cells and suppress the inflammatory response induced by cell necrosis; , and experiments illustrated its low cytotoxicity and excellent tumor inhibitory effect.
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http://dx.doi.org/10.1039/d2cc01729fDOI Listing
August 2022

Multi-organs perioperative immune-related adverse events and postoperative bronchial anastomotic fistula in a patient receiving neoadjuvant immunotherapy with NSCLC.

Authors:
Yuan Xu Xiaohong Lyu Yingzhi Qin Dongjie Ma Mengzhao Wang Juhong Shi Yun Long Bo Tang Hongsheng Liu

Thorac Cancer 2022 Jul 11. Epub 2022 Jul 11.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China.

The safety of neoadjuvant chemoimmunotherapy before surgery in patients with non-small cell lung cancer (NSCLC) remains unclear in the perioperative stage. We describe a case of a 63-year-old man with IIIC stage NSCLC who received neoadjuvant chemoimmunotherapy and radical lobectomy. After the second cycle of pembrolizumab and chemotherapy (paclitaxel + carboplatin), the patient was diagnosed with immunologic enterocolitis and relieved by glucocorticoid therapy. Radical lobectomy of the right upper lobe was then performed. On postoperative day 4 (POD 4), the patient suddenly suffered suffocated wheezing during sleep. Interstitial lung disease was, therefore, identified by chest computed tomography scan. Glucocorticoids and mechanical ventilation were applied and the symptoms were relieved. On POD 10, the patient developed a bronchial fistula and underwent emergent repair surgery. This is the first case of multi-organs, multi-time point immune-related adverse events (irAE) in perioperative NSCLC patients who received neoadjuvant chemoimmunotherapy. Clinicians should be on high alert for signs of irAEs in neoadjuvant chemoimmunotherapy patients, promptly requiring multidisciplinary management.
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http://dx.doi.org/10.1111/1759-7714.14567DOI Listing
July 2022

Dual near infrared emission in AgSe quantum dots Pb doping for broadband mini light-emitting diodes.

Authors:
Liping Wang Xinyi Cao Zaiqi Liu Yuqiong Wang Puxian Xiong Xiaoman Li Wen Gao Bo Tang

Chem Commun (Camb) 2022 Jul 26;58(60):8432-8435. Epub 2022 Jul 26.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, China.

Dual emission almost covering the entire NIR-II region with the maximum full width at half maximum of 542 nm was achieved by doping small amounts of Pb ions into AgSe quantum dots, which to the best of our knowledge has not been realized for continuous tunable dual NIR emission in Ag-based QDs. The fabricated broadband NIR mini light-emitting diodes based on the Pb-doped AgSe QDs exhibit potential applications for highly sensitive and multispectral non-invasive imaging and NIR lighting.
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http://dx.doi.org/10.1039/d2cc02804bDOI Listing
July 2022

Precision Navigation of Hepatic Ischemia-Reperfusion Injury Guided by Lysosomal Viscosity-Activatable NIR-II Fluorescence.

Authors:
Jihong Liu Wen Zhang Chunmiao Zhou Mengmei Li Xin Wang Wei Zhang Zhenzhen Liu Luling Wu Tony D James Ping Li Bo Tang

J Am Chem Soc 2022 Aug 6;144(30):13586-13599. Epub 2022 Jul 6.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

Hepatic ischemia-reperfusion injury (HIRI) is responsible for postoperative liver dysfunction and liver failure. Precise and rapid navigation of HIRI lesions is critical for early warning and timely development of pretreatment plans. Available methods for assaying liver injury fail to provide the exact location of lesions in real time intraoperatively. HIRI is intimately associated with oxidative stress which impairs lysosomal degradative function, leading to significant changes in lysosomal viscosity. Therefore, lysosomal viscosity is a potential biomarker for the precise targeting of HIRI. Hence, we developed a viscosity-activatable second near-infrared window fluorescent probe (NP-V) for the detection of lysosomal viscosity in hepatocytes and mice during HIRI. A reactive oxygen species-malondialdehyde-cathepsin B signaling pathway during HIRI was established. We further conducted high signal-to-background ratio NIR-II fluorescence imaging of HIRI mice. The contour and boundary of liver lesions were delineated, and as such the precise intraoperative resection of the lesion area was implemented. This research demonstrates the potential of NP-V as a dual-functional probe for the elucidation of HIRI pathogenesis and the direct navigation of HIRI lesions in clinical applications.
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http://dx.doi.org/10.1021/jacs.2c03832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354259PMC
August 2022

Photocontrollable Fluorescence Imaging of Mitochondrial Peroxynitrite during Ferroptosis with High Fidelity.

Authors:
Xilei Xie Yawen Liu Guangzhao Liu Yuying Zhao Jie Bian Yong Li Jian Zhang Xu Wang Bo Tang

Anal Chem 2022 07 6;94(28):10213-10220. Epub 2022 Jul 6.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, P. R. China.

Ferroptosis, a new regulatory cell death modality, underlies the pathogenesis of a broad range of disorders. Although much efforts have been made to uncover the molecular mechanisms, some mechanistic details of ferroptosis still remain poorly understood. Particularly, the functional relevance of mitochondrial reactive oxygen species (ROS) in ferroptosis is still highly controversial, which is partially due to the fact that it still remains puzzled how the mitochondrial ROS level varies during ferroptosis. The conventional mitochondria-targeted probes may react with cytosolic ROS and show fluorescence variation before entering mitochondria, thus probably giving a false result on the mitochondrial ROS level and leading to the misjudgment on its biofunction. To circumvent this issue, we rationally designed a photocontrollable and mitochondria-targeted fluorescent probe to in situ visualize the mitochondrial peroxynitrite (ONOO), which is the ROS member and mediator of ferroptosis. The photoactivated probe was endowed with a highly specific and sensitive fluorescence response to ONOO. Notably, the response activity could be artificially regulated with light irradiation, which ensured that all the probe molecules passed through the cytosol in the locked status and were then photoactivated after reaching mitochondria. This photocontrolled fluorescence imaging strategy eliminated the interference of ONOO outside the mitochondria, thus potentially afforded improved fidelity for mitochondrial ONOO bioimaging in live cells and animal models. With this probe, for the first time, we revealed the mitochondrial ONOO flux and its probable biological source during erastin-induced ferroptosis. These results suggest a tight correlation between mitochondrial ONOO/ROS and ferroptotic progression, which will further facilitate the comprehensive exploration and manipulation of ferroptosis.
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http://dx.doi.org/10.1021/acs.analchem.2c01758DOI Listing
July 2022

Waveguide-Integrated PdSe Photodetector over a Broad Infrared Wavelength Range.

Authors:
Jianghong Wu Hui Ma Chuyu Zhong Maoliang Wei Chunlei Sun Yuting Ye Yan Xu Bo Tang Ye Luo Boshu Sun Jialing Jian Hao Dai Hongtao Lin Lan Li

Nano Lett 2022 Jul 5. Epub 2022 Jul 5.

Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province, School of Engineering, Westlake University, Hangzhou 310024, China.

Hybrid integration of van der Waals materials on a photonic platform enables diverse exploration of novel active functions and significant improvement in device performance for next-generation integrated photonic circuits, but developing waveguide-integrated photodetectors based on conventionally investigated transition metal dichalcogenide materials at the full optical telecommunication bands and mid-infrared range is still a challenge. Here, we integrate PdSe with silicon waveguide for on-chip photodetection with a high responsivity from 1260 to 1565 nm, a low noise-equivalent power of 4.0 pW·Hz, a 3-dB bandwidth of 1.5 GHz, and a measured data rate of 2.5 Gbit·s. The achieved PdSe photodetectors provide new insights to explore the integration of novel van der Waals materials with integrated photonic platforms and exhibit great potential for diverse applications over a broad infrared range of wavelengths, such as on-chip sensing and spectroscopy.
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http://dx.doi.org/10.1021/acs.nanolett.2c02099DOI Listing
July 2022

Oncogenic role and potential regulatory mechanism of topoisomerase IIα in a pan-cancer analysis.

Authors:
Xiaobo Wang Jinhua Wang Li Lyu Xin Gao Yinuo Cai Bo Tang

Sci Rep 2022 Jul 1;12(1):11161. Epub 2022 Jul 1.

Department of Hematology, The Second Affiliated Hospital of Dalian Medical University, Liaoning, Dalian, People's Republic of China.

Topoisomerase IIα (TOP2A) plays an oncogenic role in multiple tumor types. However, no pan-cancer analysis about the function and the upstream molecular mechanism of TOP2A is available. For the first time, we analyzed potential oncogenic roles of TOP2A in 33 cancer types via The Cancer Genome Atlas (TCGA) database. Overexpression of TOP2A was existed in almost all cancer types, and related to poor prognosis and advanced pathological stages in most cases. Besides, the high frequency of TOP2A genetic alterations was observed in several cancer types, and related to prognosis in some cases. Moreover, we conduct upstream miRNAs and lncRNAs of TOP2A to establish ceRNA networks in kidney renal clear cell carcinoma (SNHG3-miR-139-5p), kidney renal papillary cell carcinoma (TMEM147-AS1/N4BP2L2-IT2/THUMPD3-AS1/ERICD/TTN-AS1/SH3BP5-AS1/THRB-IT1/SNHG3/NEAT1-miR-139-5p), liver hepatocellular carcinoma (SNHG3/THUMPD3-AS1/NUTM2B-AS1/NUTM2A-AS1-miR-139-5p and SNHG6/GSEC/SNHG1/SNHG14/LINC00265/MIR3142HG-miR-101-3p) and lung adenocarcinoma (TYMSOS/HELLPAR/SNHG1/GSEC/SNHG6-miR-101-3p). TOP2A expression was generally positively correlated with cancer associated fibroblasts, M0 and M1 macrophages in most cancer types. Furthermore, TOP2A was positively associated with expression of immune checkpoints (CD274, CTLA4, HAVCR2, LAG3, PDCD1 and TIGIT) in most cancer types. Our first TOP2A pan-cancer study contributes to understanding the prognostic roles, immunological roles and potential upstream molecular mechanism of TOP2A in different cancers.
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http://dx.doi.org/10.1038/s41598-022-15205-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249858PMC
July 2022

Cu Embedded Three-Dimensional Covalent Organic Framework for Multiple ROS-Based Cancer Immunotherapy.

Authors:
Xia Zhang Shenglin Wang Kun Tang Wei Pan Huanjun Xu Yanhua Li Yanan Gao Na Li Bo Tang

ACS Appl Mater Interfaces 2022 Jul 28;14(27):30618-30625. Epub 2022 Jun 28.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

Reactive oxygen species (ROS)-based cancer treatments have attracted much attention in recent years. However, most patients respond poorly to the monotypic ROS during these treatments. In this work, a multiple ROS-based cancer immunotherapy synergistic strategy has been developed to enhance the therapeutic effect of cancer. We prepare a three-dimensional covalent organic framework (3D COF-TATB), and embed copper ions (Cu) into the skeleton to obtain multifunctional nanomaterial, 3D [email protected] In this system, porphyrins in 3D COF-TATB serve not only as the photosensitizer for photodynamic process to produce singlet oxygen(O), but also as the binding sites to complex with Cu. Cu can be reduced by the GSH to generate Cu to produce hydroxyl radical (•OH) through the Fenton-like reaction. Moreover, the generated multiple types of ROS induce the immunogenic cell death (ICD) of cancer cells to improve the immunogenicity and further activate an immune response for attacking the tumor. Combining with the immunoblocking inhibitor (aPD-1), 3D [email protected] can effectively inhibit the tumor growth. This work will provide a guidance for multimodal cancer therapy in future clinical treatment settings.
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http://dx.doi.org/10.1021/acsami.2c07739DOI Listing
July 2022

Hypochlorous Acid-Activated Multifunctional Fluorescence Platform for Depression Therapy and Antidepressant Efficacy Evaluation.

Authors:
Xin Wang Ruiyuan Wang Qi Ding Wei Wu Feida Che Ping Li Wen Zhang Wei Zhang Zhenzhen Liu Bo Tang

Anal Chem 2022 07 28;94(27):9811-9818. Epub 2022 Jun 28.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

Current diagnosis of depression rests on the symptoms, so it still lacks objective criteria. Meanwhile, existing treatment of depression is dominated by antidepressants, which produce troublesome side effects and usually require months to achieve effect. Therefore, more reliable diagnostic criteria and effective therapy are urgently needed. Some core hallmarks in the etiology of depression have been established, including declined neurotransmitters and inflammatory responses, manifesting in oxidative stress. Thus, we fabricated a HClO-triggered multifunctional fluorescence platform (MB-Rs) for simultaneous neurotransmitter/antidepressant delivery and efficacy evaluation. In MB-Rs, although a urea linkage could be specifically cut off by HClO, methylene blue (MB) endowed with excellent anti-inflammatory and optical properties was covalently linked with neurotransmitters (dopamine or 5-hydroxytryptamine) or antidepressants (fluoxetine). Encountering excess HClO in the brain of mice with depression, MB-Rs released corresponding antidepressants and MB with anti-inflammatory and bright fluorescence. By relieving oxidative stress, inflammation, and coinstantaneous increasing neurotransmitters, MB-Rs elicited better antidepressant response and fewer side effects compared with clinical antidepressants. Furthermore, MB-Rs successfully evaluated the efficacy of antidepressants in mice based on HClO-induced fluorescence. Therefore, this work provides a promising platform for depression diagnosis and treatment.
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http://dx.doi.org/10.1021/acs.analchem.2c01639DOI Listing
July 2022

TLR4 regulates RORγt regulatory T-cell responses and susceptibility to colon inflammation through interaction with Akkermansia muciniphila.

Authors:
Yaojiang Liu Min Yang Li Tang Fengchao Wang Shengjie Huang Shuang Liu Yuanyuan Lei Sumin Wang Zhuo Xie Wei Wang Xiaoyan Zhao Bo Tang Shiming Yang

Microbiome 2022 06 27;10(1):98. Epub 2022 Jun 27.

Department of Gastroenterology, The Second Affiliated Hospital of Third Military Medical University, 400037, Chongqing, China.

Background: Well-balanced interactions between gut microbiota and the immune system are essential to prevent chronic intestinal inflammation, as observed in inflammatory bowel diseases (IBD). Toll-like receptor 4 (TLR4) functions as a sensor mediating the crosstalk between the intestinal commensal microbiome and host immunity, but the influence of TLR4 on the shaping of intestinal microbiota and immune responses during colon inflammation remains poorly characterized. We investigated whether the different susceptibilities to colitis between wild-type (WT) and TLR4 mice were gut microbiota-dependent and aimed to identify the potential immunity modulation mechanism.

Methods: We performed antibiotic depletion of the microbiota, cohousing experiments, and faecal microbiota transplantation (FMT) in WT and TLR4 mice to assess the influence of TLR4 on intestinal microbial ecology. 16S rRNA sequencing was performed to dissect microbial discrepancies, and dysbiosis-associated immune perturbation was investigated by flow cytometry. Akkermansia muciniphila (A. muciniphila)-mediated immune modulation was confirmed through the T-cell transfer colitis model and bone marrow chimaera construction.

Results: TLR4 mice experienced enhanced susceptibility to DSS-induced colitis. 16S rRNA sequencing showed notable discrepancy in the gut microbiota between WT and TLR4 mice. In particular, A. muciniphila contributed most to distinguishing the two groups. The T-cell transfer colitis model and bone marrow transplantation (BMT) consistently demonstrated that A. muciniphila ameliorated colitis by upregulating RORγt Treg cell-mediated immune responses. Mucosal biopsies from human manifested parallel outcomes with colon tissue from WT mice, as evidenced by the positive correlation between TLR4 expression and intestinal A. muciniphila colonization during homeostasis.

Conclusions: Our results demonstrate a novel protective role of TLR4 against intestinal inflammation, wherein it can modulate A. muciniphila-associated immune responses. These findings provide a new perspective on host-commensal symbiosis, which may be beneficial for developing potential therapeutic strategies. Video abstract.
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http://dx.doi.org/10.1186/s40168-022-01296-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235089PMC
June 2022

Corrigendum to "Long noncoding RNA LINC00675 enhances phosphorylation of vimentin on Ser83 to suppress gastric cancer progression" [Cancer Lett. 2018 Jan 1;412:179-187].

Authors:
Shuo Zeng Xia Xie Yu-Feng Xiao Bo Tang Chang-Jiang Hu Shu-Ming Wang Yu-Yun Wu Hui Dong Bo-Sheng Li Shi-Ming Yang

Cancer Lett 2022 Sep 25;543:215805. Epub 2022 Jun 25.

Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China. Electronic address:

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http://dx.doi.org/10.1016/j.canlet.2022.215805DOI Listing
September 2022

Effect of Surface Charge Characteristics of Ferroelectric LiNbO on Wettability of Ionic Liquids.

Authors:
Bo Tang Yiwen Zhao Sen Yang Zhiang Guo Zhenhui Wang An Xing Xiaoyan Liu

Nanomaterials (Basel) 2022 Jun 17;12(12). Epub 2022 Jun 17.

Chongqing Key Laboratory of Nano/Micro Composites and Devices, College of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing 401331, China.

Electrowetting is a widely used and effective method to tune the wettability of ionic liquids at solid-liquid interfaces, but it usually requires an external electric field. Here, we proposed a strategy for conveniently tuning ionic liquid wettability by adopting ferroelectric LiNbO single crystals as functional substrates. A heating pretreatment process was applied to modulate the surface charge characteristics of LiNbO substrates, leading to an improved wettability of [EMIM][BF] and [EMIM][NTf] on the LiNbO substrates with both positively poled (+Z) and negatively poled (-Z) surfaces. This work may be of great interest in the field of ferroelectric-based microelectronics.
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http://dx.doi.org/10.3390/nano12122085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228295PMC
June 2022

Simultaneous fluorescence imaging of Golgi O and Golgi HO in mice with hypertension.

Authors:
Hui Wang Cuifang Liu Xiaoting Zhang Tiancong Xiu Ping Li Wei Zhang Wen Zhang Xin Wang Zhenzhen Liu Bo Tang

Biosens Bioelectron 2022 Oct 17;213:114480. Epub 2022 Jun 17.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Minis-try of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan, 250014, People's Republic of China. Electronic address:

Hypertensive cardiovascular disease is a persistent threat to public health. Elucidating the pathogenesis of hypertension is expected to provide more highly targeted therapies for patients. To date, reactive oxygen species (ROS) induced endothelial nitric oxide synthase (eNOS) uncoupling are generally considered to be common phenomena in hypertension. However, the critical factor contribute to persistent eNOS uncoupling remains poorly understood. Herein, we established a fluorescence probe, Gol, for the multicolored and simultaneous detection of Golgi O and HO in situ. We successfully detected increases in Golgi ROS levels in hypertensive mice and evaluated the pharmaceutical effects of various antihypertensive drugs. More importantly, we identified the ROS post-transcriptional modification sites on dihydrofolate reductase (DHFR). Altogether, we propose a novel therapeutic target for hypertension, which will promote the development of new antihypertensive drugs, and also developed an ideal fluorescence probe to study in situ Golgi O and HO changes in various biochemical processes.
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http://dx.doi.org/10.1016/j.bios.2022.114480DOI Listing
October 2022

The downregulation of miR-22 and miR-372 may contribute to gestational diabetes mellitus through regulating glucose metabolism via the PI3K/AKT/GLUT4 pathway.

Authors:
Wei Li Xianlin Yuan Xin He Li Yang Yingyuan Wu Xiaofeng Deng Yiwen Zeng Kesheng Hu Bo Tang

J Clin Lab Anal 2022 Jul 17;36(7):e24557. Epub 2022 Jun 17.

Department of Clinical Pharmacy, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Identifying effective regulatory mechanisms will be significant for Gestational diabetes mellitus (GDM) diagnosis and treatment.

Methods: The expressions of miR-22 and miR-372 in placenta tissues from 75 pregnant women with GDM and 75 matched healthy controls and HRT8/SVneo cells (a model of insulin resistance) were analyzed by qPCR. The expressions of PI3K, AKT, IRS, and GLUT4 in high glucose-treated HRT8/SVneo cells transfected with miR-22 or miR-372 mimics or inhibitors was assessed by Western blot. A luciferase gene reporter assay was employed to verify miRNAs' target genes.

Results: The expressions of miR-22 and miR-372 in placental tissues from GDM patients and HRT8/SVneo cells were significantly decreased compared with the respective controls. The GLUT4 expression was significantly decreased in the placenta tissues of GDM and HRT8/SVneo cells with high glucose transfected with miR-22 and miR-372 inhibitors. We confirmed that SLC2A4, the gene encoding GLUT4, was a direct target of miR-22 and miR-372. In this study, we report that the lower expressions of miR-22 and miR-372 in placental tissue from GDM patients.

Conclusion: Our results further suggested that the downregulations of miR-22 and miR-372 may contribute to GDM through regulating the PI3K/GLUT4 pathway.
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http://dx.doi.org/10.1002/jcla.24557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279990PMC
July 2022

Application of da Vinci robot and laparoscopy on repeat hepatocellular carcinoma.

Authors:
Shuiping Yu Guandou Yuan Shiliu Lu Jiangfa Li Bo Tang Fudi Zhong Huizhao Su Songqin He

J Minim Access Surg 2022 Jul-Sep;18(3):378-383

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Background: Repeat laparoscopic liver resection has been used safely and effectively on hepatocellular carcinoma (HCC). However, few studies have been performed on repeat HCC surgery by a da Vinci robot. This study aims to evaluate the outcomes of the patients with repeat HCC treated using a da Vinci robot or laparoscopic system at a single centre.

Methods: All of the patients with repeat HCC treated using a da Vinci robotic or laparoscopic system between April 2017 and April 2020 were included in this retrospective study.

Results: There were 24 patients with a mean age of 56 years who underwent da Vinci robotic or laparoscopic surgery for treatment of repeat HCC who were included in this study. The operations lasted 152 ± 25 min and 142 ± 34 min. The average intraoperative blood loss was 284 ± 89 ml and 251 ± 92 ml. The average hospitalisation stay lasted 9 ± 2 days and 9 ± 3 days. The rates at which surgeons switched to open surgery were 9% and 23%. No serious perioperative or post-operative complications were encountered.

Conclusion: Da Vinci robots can provide a precise dissection of the tissue under a perfect view. It is a technically feasible procedure for less rates at which surgeons switched to open surgery on repeat HCC.
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http://dx.doi.org/10.4103/jmas.JMAS_111_21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306131PMC
June 2022

Exosomal circRNA FNDC3B promotes the progression of esophageal squamous cell carcinoma by sponging miR-490-5p and regulating thioredoxin reductase 1 expression.

Authors:
Bo Tang Qingfeng Zhang Kui Liu Yun Huang

Bioengineered 2022 05;13(5):13829-13848

Department of Cardio-Thoracic Surgery, Zigong Fourth People's Hospital, Zigong City, China.

Exosomal circular RNAs (circRNAs) have been reported to play critical roles in esophageal squamous cell carcinoma (ESCC). We aimed to investigate the function of exosomal circRNA FNDC3B (circFNDC3B). The RNA levels and protein levels were examined using RT-qPCR and western blot (WB) assays. Colony formation and EdU assays were used to assess cell proliferative ability. Cell migratory and invasive abilities were detected by wound healing and transwell assays. Cell apoptosis was measured by flow cytometry. Glycolysis was measured using commercial kits. Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) were applied to examine the morphology and size of exosomes. Dual-luciferase reporter, RIP and RNA pull-down assays assessed the interaction of miR-490-5p with circFNDC3B or thioredoxin reductase 1 (TXNRD1). Xenograft tumor model determined the role of exosomal circFNDC3B . We observed that circFNDC3B was upregulated in ESCC samples and cells, as well as ESCC-derived exosomes. CircFNDC3B could be delivered via exosomes in tumor cells, and the colony formation, proliferation, migration, invasion, glycolysis, and growth ability of recipient cells were weakened after co-incubation with exosomal circFNDC3B-knockdown donor cells. CircFNDC3B was a miR-490-5p sponge, and miR-490-5p inhibition reversed the role of exosomal circFNDC3B-downregulating in ESCC cells. TXNRD1 was a miR-490-5p target, and TXNRD1 elevation weakened the anti-cancer function of miR-490-5p upregulation in ESCC cells. CircFNDC3B mediated TXNRD1 expression by interacting with miR-490-5p. In conclusion, exosomal circFNDC3B drove ESCC progression via regulating the miR-490-5p/TXNRD1 axis.AbbreviationsEC: esophageal cancer; ESCC: esophageal squamous cell carcinoma; circRNA: circular RNA; WB: western blot; TEM: transmission electron microscopy; NTA: nanoparticle tracking analysis; TXNRD1: thioredoxin reductase 1; IHC: immunohistochemistry; RT-qPCR: reverse transcription-polymerase quantitative chain reaction; GLUT1: glucose transport protein type 1; LDHA: lactate dehydrogenase A.
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http://dx.doi.org/10.1080/21655979.2022.2084484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275986PMC
May 2022

Comprehensive Analyses of MELK-Associated ceRNA Networks Reveal a Potential Biomarker for Predicting Poor Prognosis and Immunotherapy Efficacy in Hepatocellular Carcinoma.

Authors:
Yu Liu Rongkuan Li Xiaobo Wang Zuguang Xue Xiaozhou Yang Bo Tang

Front Cell Dev Biol 2022 27;10:824938. Epub 2022 May 27.

Department of Hematology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world with high morbidity and mortality. Identifying specific molecular markers that can predict HCC prognosis is extremely important. MELK has been reported to play key roles in several types of human cancers and predict poor prognosis. This study was aimed to explore the impact of MELK on HCC. A pan-cancer analysis of MELK was conducted by The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) data. The prognosis of MELK in various cancers was analyzed in GEPIA. Then, a ceRNA network of MELK was constructed based on the comprehensive consideration of the expression analysis, the correlation analysis, and the survival analysis by R software. The correlation of MELK and immune cell infiltration was analyzed by TIMER and CIBERSORT. Then, the overall survival of differentially expressed immune cells was conducted. The correlation of MELK and immune checkpoints expression was analyzed by GEPIA. MELK was overexpressed in 14 types of human cancers, and its expression was significantly higher than that in both unmatched and paired normal samples in HCC. Higher MELK expression was correlated with poorer survival and advanced clinical stage, topography (T) stage, and histological grade. The univariate and multivariate Cox regression analyses showed that MELK was an independent risk factor for poor prognosis in HCC. Then, we constructed a ceRNA network consisting of MELK, miR-101-3p, and two lncRNAs (SNHG1 and SNHG6) after evaluating the expression and impact on prognosis in HCC of these RNAs. TIMER and CIBERSORT databases indicated that MELK was correlated with various immune cells including B cells, CD8 T cells, CD4 T cells, macrophage, neutrophil, and dendritic cells in HCC. Of them, B cells, CD4 T cells, macrophage, and neutrophil were related to the prognosis of HCC. In addition, MELK was significantly positively correlated with the immune checkpoint genes. : MELK may be a novel potential biomarker for predicting prognosis and immunotherapy efficacy in patients with HCC. Our study may provide new molecular and therapeutic strategies for the treatment of HCC patients.
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http://dx.doi.org/10.3389/fcell.2022.824938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184526PMC
May 2022

Quantum Dots with a Compact Amphiphilic Zwitterionic Coating.

Authors:
Bo Tang Bing-Hua Liu Zhen-Ya Liu Meng-Yao Luo Xue-Hui Shi Dai-Wen Pang

ACS Appl Mater Interfaces 2022 Jun 10;14(24):28097-28104. Epub 2022 Jun 10.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.

Generally speaking, it is difficult to keep nanomaterials encapsulated in amphiphilic polymers like octylamine-grafted poly(acrylic acid) (OPA) compact in coating-layer, with a small hydrodynamic size. Here, we prepared stable hydrophilic quantum dots (QDs) via encapsulation in ∼3 nm-long amphiphilic and zwitterionic (AZ) molecules. After encapsulation with AZ molecules, the coated QDs are only 2.1 nm thicker in coating, instead of 5.4 nm with OPA. Meanwhile, the hydrodynamic sizes of CdSe/CdS, ZnCdSeS, ZnCdSe/ZnS, and CdSe/ZnS QDs encapsulated in AZ molecules (AZ-QDs) are less than 15 nm, and 6-7 nm smaller than those of QDs in OPA (OPA-QDs). Notably, both extracellular and intracellular nonspecific binding of AZ-QDs is approximately 100-folds lower than that of OPA-QDs.
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http://dx.doi.org/10.1021/acsami.2c04438DOI Listing
June 2022

Population pharmacokinetics and dosing optimization of mezlocillin in neonates and young infants.

Authors:
Jing Zhou Li Jiang Zhi Ling Zhang Zhao Rui Wang Yan Xiu Zhang Xu Lin Bo Hao Tang Bu Fan Yao Zi Xuan Guo Jing Jing Yang John Van Den Anker Yue E Wu Wei Zhao

J Antimicrob Chemother 2022 Jul;77(8):2238-2244

Department of Clinical Pharmacy, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objectives: Mezlocillin is used in the treatment of neonatal infectious diseases. However, due to the absence of population pharmacokinetic studies in neonates and young infants, dosing regimens differ considerably in clinical practice. Hence, this study aimed to describe the pharmacokinetic characteristics of mezlocillin in neonates and young infants, and propose the optimal dosing regimen based on the population pharmacokinetic model of mezlocillin.

Methods: A prospective, open-label pharmacokinetic study of mezlocillin was carried out in newborns. Blood samples were collected using an opportunistic sampling method. HPLC was used to measure the plasma drug concentrations. A population pharmacokinetic model was developed using NONMEM software.

Results: Ninety-five blood samples from 48 neonates and young infants were included. The ranges of postmenstrual age and birth weight were 29-40 weeks and 1200-4000 g, respectively, including term and preterm infants. A two-compartment model with first-order elimination was developed to describe the population pharmacokinetics of mezlocillin. Postmenstrual age, current weight and serum creatinine concentration were the most important covariates. Monte Carlo simulation results indicated that the current dose of 50 mg/kg q12h resulted in 89.2% of patients achieving the therapeutic target, when the MIC of 4 mg/L was used as the breakpoint. When increasing the dosing frequency to q8h, a dose of 20 mg/kg resulted in 74.3% of patients achieving the therapeutic target.

Conclusions: A population pharmacokinetic model of mezlocillin in neonates and young infants was established. Optimal dosing regimens based on this model were provided for use in neonatal infections.
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http://dx.doi.org/10.1093/jac/dkac176DOI Listing
July 2022
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