Publications by authors named "Bo Song"

909 Publications

Abnormally elevated ubiquilin‑1 expression in breast cancer regulates metastasis and stemness via AKT signaling.

Oncol Rep 2021 Nov 16;46(5). Epub 2021 Sep 16.

Department of Pathology and Forensic Medicine, College of Basic Medical Science, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.

Ubiquilin‑1 (UBQLN1) is an essential factor for the maintenance of proteostasis in cells. It is important for the regulation of different protein degradation mechanisms, including the ubiquitin‑proteasome system, autophagy and endoplasmic reticulum‑associated protein degradation pathways. However, the role of UBQLN1 in cancer progression remains largely unknown. In the present study, the expression, functions and molecular mechanisms of UBQLN1 in breast cancer tissue samples and cell lines were explored. Immunohistochemical and bioinformatics analyses revealed that UBQLN1 expression was significantly upregulated in breast cancer tissues and cell lines. UBQLN1 expression in breast cancer was significantly associated with lymph node metastasis and TNM stage. Moreover, a high UBQLN1 expression was a predictor of an unfavorable survival in patients with breast cancer. , UBQLN1 silencing markedly inhibited cell migration and invasion, epithelial‑to‑mesenchymal transition (EMT) and MMP expression. UBQLN1 silencing attenuated the stem cell‑like properties of breast cancer cells, including their mammosphere‑forming abilities. UBQLN1 knockdown also enhanced breast cancer cell chemosensitivity to paclitaxel. The expression levels of the stem cell markers. Aldehyde dehydrogenase 1 (ALDH1), Oct‑4 and Sox2 were significantly decreased in the cells in which UBQLN1 was silenced, whereas breast cancer stem cells exhibited an increased expression of UBQLN1. Mechanistically, UBQLN1 knockdown inhibited the activation of AKT signaling, as revealed by the increased PTEN expression and the decreased expression of phosphorylated AKT in cells in which UBQLN1 was silenced. On the whole, the present study demonstrates that UBQLN1 is aberrantly upregulated in breast cancer and predicts a poor prognosis. The silencing of UBQLN1 inhibited the invasion, EMT and stemness of breast cancer cells, possibly via AKT signaling.
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http://dx.doi.org/10.3892/or.2021.8187DOI Listing
November 2021

Mapping causal genes and genetic interactions for agronomic traits using a large F2 population in rice.

G3 (Bethesda) 2021 Sep 13. Epub 2021 Sep 13.

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

Dissecting the genetic mechanisms underlying agronomic traits is of great importance for crop breeding. Agronomic traits are usually controlled by multiple quantitative trait loci (QTLs) and genetic interactions, and mapping the underlying causal genes is still labor-intensive and time-consuming. Here, we present a genetic tool for directly targeting the specific causal genes by using a single-gene resolution linkage map that was constructed from 3,756 F2 rice plants via targeted sequencing technology and Tukey-Kramer multiple comparisons test. Three large- and moderate-effect QTLs, qHD6-2, qGL3-1, and qGW5-2, were successfully mapped to their specific causal genes, Hd1, GS3, and GW5, respectively. A complex genetic interaction network containing 30 QTL-QTL interactions was constructed, revealing that the alternative allele of hub QTL, qHD6-2, can hide or release the genetic contributions of the alleles at interacting loci. Moreover, arranging genetic interactions in the models lead to more accurate phenotypic predictions. These results provide a community resource and new feasible strategy for deciphering the genetic mechanisms of complex agronomic traits and accelerating crop breeding.
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http://dx.doi.org/10.1093/g3journal/jkab318DOI Listing
September 2021

PCage: Fluorescent Molecular Temples for Binding Sugars in Water.

J Am Chem Soc 2021 Sep 10. Epub 2021 Sep 10.

Department of Chemistry, 2145 Sheridan Road, Northwestern University, Evanston, Illinois 60208, United States.

The development of synthetic receptors that recognize carbohydrates in water with high selectivity and specificity is challenging on account of their structural complexity and strong hydrophilicity. Here, we report on the design and synthesis of two pyrene-based, temple-shaped receptors for the recognition of a range of common sugars in water. These receptors rely on the use of two parallel pyrene panels, which serve as roofs and floors, capable of forming multiple [C-H···π] interactions with the axially oriented C-H bonds on glycopyranosyl rings in the carbohydrate-based substrates. In addition, eight polarized pyridinium C-H bonds, projecting from the roofs and floors of the temple receptors toward the binding cavities, form [C-H···O] hydrogen bonds, with the equatorially oriented OH groups on the sugars located inside the hydrophobic cavities. Four -xylylene pillars play a crucial role in controlling the distance between the roof and floor. These temple receptors are highly selective for the binding of glucose and its derivatives. Furthermore, they show enhanced fluorescence upon binding with glucose in water, a property which is useful for glucose-sensing in aqueous solution.
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http://dx.doi.org/10.1021/jacs.1c06333DOI Listing
September 2021

HMB Improves Lipid Metabolism of Bama Xiang Mini-Pigs via Modulating the -Acetic Acid-AMPKα Axis.

Front Microbiol 2021 16;12:736997. Epub 2021 Aug 16.

CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Here, we used Bama Xiang mini-pigs to explore the effects of different dietary β-hydroxy-β-methylbutyrate (HMB) levels (0, 0.13, 0.64 or 1.28%) on lipid metabolism of adipose tissue. Results showed that HMB decreased the fat percentage of pigs (linearly, < 0.05), and the lowest value was observed in the 0.13% HMB group. Moreover, the colonic acetic acid concentration and the relative abundance were increased in response to HMB supplementation ( < 0.05). Correlation analysis identified a positive correlation between the relative abundance and acetic acid production, and a negative correlation between fat percentage and the relative abundance or acetic acid production. HMB also upregulated the phosphorylation (p) of AMPKα, Sirt1, and FoxO1, and downregulated the p-mTOR expression. Collectively, these findings indicate that reduced fat percentage in Bama Xiang mini-pigs could be induced by HMB supplementation and the mechanism might be associated with the -acetic acid-AMPKα axis.
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http://dx.doi.org/10.3389/fmicb.2021.736997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415715PMC
August 2021

Fabrication of decellularized meniscus extracellular matrix according to inner cartilaginous, middle transitional, and outer fibrous zones result in zone-specific protein expression useful for precise replication of meniscus zones.

Mater Sci Eng C Mater Biol Appl 2021 Sep 14;128:112312. Epub 2021 Jul 14.

Department of Orthopedic Surgery, School of Medicine, Ajou University, Suwon, Republic of Korea; Cell Therapy Center, Ajou Medical Center, Suwon, Republic of Korea. Electronic address:

Meniscus is a fibrocartilage composite tissue with three different microstructual zones, inner fibrocartilage, middle transitional, and outer fibrous zone. We hypothesized that decellularized meniscus extracellular matrix (DMECM) would have different characteristics according to zone of origin. We aimed to compare zone-specific DMECM in terms of biochemical characteristics and cellular interactions associated with tissue engineering. Micronized DMECM was fabricated from porcine meniscus divided into three microstructural zones. Characterization of DMECM was done by biochemical and proteomic analysis. Inner DMECM showed the highest glycosaminoglycan content, while middle DMECM showed the highest collagen content among groups. Proteomic analysis showed significant differences among DMECM groups. Inner DMECM showed better adhesion and migration potential to meniscus cells compared to other groups. DMECM resulted in expression of zone-specific differentiation markers when co-cultured with synovial mesenchymal stem cells (SMSCs). SMSCs combined with inner DMECM showed the highest glycosaminoglycan in vivo. Outer DMECM constructs, on the other hand, showed more fibrous tissue features, while middle DMECM constructs showed both inner and outer zone characteristics. In conclusion, DMECM showed different characteristics according to microstructural zones, and such material may be useful for zone-specific tissue engineering of meniscus.
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http://dx.doi.org/10.1016/j.msec.2021.112312DOI Listing
September 2021

A contorted nanographene shelter.

Nat Commun 2021 08 31;12(1):5191. Epub 2021 Aug 31.

Department of Chemistry, Northwestern University, Evanston, IL, USA.

Nanographenes have kindled considerable interest in the fields of materials science and supramolecular chemistry as a result of their unique self-assembling and optoelectronic properties. Encapsulating the contorted nanographenes inside artificial receptors, however, remains challenging. Herein, we report the design and synthesis of a trigonal prismatic hexacationic cage, which has a large cavity and adopts a relatively flexible conformation. It serves as a receptor, not only for planar coronene, but also for contorted nanographene derivatives with diameters of approximately 15 Å and thicknesses of 7 Å. A comprehensive investigation of the host-guest interactions in the solid, solution and gaseous states by experimentation and theoretical calculations reveals collectively an induced-fit binding mechanism with high binding affinities between the cage and the nanographenes. Notably, the photostability of the nanographenes is improved significantly by the ultrafast deactivation of their excited states within the cage. Encapsulating the contorted nanographenes inside the cage provides a noncovalent strategy for regulating their photoreactivity.
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http://dx.doi.org/10.1038/s41467-021-25255-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408160PMC
August 2021

Novel assessment of leukocyte-rich platelet-rich plasma on functional and patient-reported outcomes in knee osteoarthritis: a pilot study.

Regen Med 2021 09 23;16(9):823-832. Epub 2021 Aug 23.

Department of Surgery, Interdisciplinary Consortium on Advanced Motion Performance (iCAMP), Baylor College of Medicine, Houston, TX 77030, USA.

Leukocyte-rich platelet-rich plasma (LR-PRP) has demonstrated to be beneficial for patients with knee osteoarthritis (KOA); however, reliable objective end points to accurately assess its therapeutic effects is lacking. To investigate the efficacy of LR-PRP as assessed by functional and patient-reported outcomes at early time points (6 weeks). We conducted a prospective cohort study in 12 patients with diagnosed KOA (Kellgren Lawrence score of II-III), who underwent a single ultrasound-guided LR-PRP injection. There was significant improvement in timed up and go, pain and quality of life scales and balance parameters. There were nonsignificant improvements in range of motion and gait parameters. LR-PRP demonstrates efficacy in meaningful end points for functional and patient-reported outcomes at early time points in patients with KOA.
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http://dx.doi.org/10.2217/rme-2021-0032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438954PMC
September 2021

Spatial organization of dinoflagellate genomes: Novel insights and remaining critical questions.

J Phycol 2021 Aug 14. Epub 2021 Aug 14.

Département de Sciences Biologiques, Institut de Recherche en Biologie Végétale, Université de Montréal, Montréal, Quebec, H1X 2B2, Canada.

As is true for many other aspects, genome architecture, evolution, and function in dinoflagellates are enigmatic and, in the meantime, continuous inspiration for scientific quests. Recent third-generation sequencing and Hi-C linkage analyses brought new insights into the spatial organization of symbiodiniacean genomes, revealing the topologically associated domains, discrete gene clusters and their cis and trans orientations, and relationships with transcription. Where do these new findings bring us in dinoflagellate genomics? Here, we aim to place these new results in the backdrop of the long history of research on this topic and in the context of what critical questions remain to be pursued in the future. The new data suggest, pending verification of other complete chromosome assemblies, a potential evolutionary trend in chromosome number decrease and length increase within the Symbiodiniaceae. While questions remain about the mechanics of the three-dimensional chromosome structure and cell cycle-related DNA replication, the mechanisms of gene transcription and genome size evolution, these latest findings set new starting points for further inquiries.
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http://dx.doi.org/10.1111/jpy.13206DOI Listing
August 2021

Efficacy of graphene oxide-loaded cationic antimicrobial peptide AWRK6 on the neutralization of endotoxin activity and in the treatment of sepsis.

Aging (Albany NY) 2021 08 13;13(15):19867-19877. Epub 2021 Aug 13.

Department of Emergency, Linyi People's Hospital, Linyi, Shandong Province, China.

Objective: This study is to assess the therapeutic effect of graphene oxide (GO) loaded with AWRK6 on endotoxin-induced sepsis.

Method: AWRK6/GO was prepared by GO loaded AWRK6, with the structure characterization of AWRK6/GO conducted by atomic force microscope (AFM) and ultraviolet spectrophotometer, the sustained release rate of AWRK6/GO detected by high performance liquid chromatography (HPLC), and the neutralization ability of AWRK6/GO to lipopolysaccharide (LPS) tested by experiments. The levels of IL-8 and TNF-α in mouse cells after drug intervention were detected by ELISA; a LPS mouse model was established to observe the effects of drug intervention on the survival cycle and survival rate of mice.

Results: The sustained drug release rate of AWRK6/GO reached 85% within 24 hours observed under conditions, with an efficient neutralization effect to LPS ( < 0.01); Compared with the control group, the intervention of LPS succeeded in remarkably elevating the levels of IL-8 and TNF-α in the whole blood and macrophages of the mice ( < 0.01), whose survival cycle and survival rate consequently observed an obvious decline ( < 0.01); The intervention with AWRK6 or AWRK6/GO predominantly brought down the levels of IL-8 and TNF-α in the whole blood and macrophages of mice given LPS ( < 0.01), resulting in an elevation of the survival rate and survival time ( < 0.01).

Conclusion: GO loaded with cationic antimicrobial peptide AWRK6 exerts a rosy neutralization effect on endotoxin activity, with no obvious side effects on mice observed, which is of certain application value in the treatment of sepsis.
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http://dx.doi.org/10.18632/aging.203397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386569PMC
August 2021

Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice.

BMC Genomics 2021 Aug 12;22(1):612. Epub 2021 Aug 12.

Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China.

Background: Soil salinization represents a serious threat to global rice production. Although significant research has been conducted to understand salt stress at the genomic, transcriptomic and proteomic levels, few studies have focused on the translatomic responses to this stress. Recent studies have suggested that transcriptional and translational responses to salt stress can often operate independently.

Results: We sequenced RNA and ribosome-protected fragments (RPFs) from the salt-sensitive rice (O. sativa L.) cultivar 'Nipponbare' (NB) and the salt-tolerant cultivar 'Sea Rice 86' (SR86) under normal and salt stress conditions. A large discordance between salt-induced transcriptomic and translatomic alterations was found in both cultivars, with more translationally regulated genes being observed in SR86 in comparison to NB. A biased ribosome occupancy, wherein RPF depth gradually increased from the 5' ends to the 3' ends of coding regions, was revealed in NB and SR86. This pattern was strengthened by salt stress, particularly in SR86. On the contrary, the strength of ribosome stalling was accelerated in salt-stressed NB but decreased in SR86.

Conclusions: This study revealed that translational reprogramming represents an important layer of salt stress responses in rice, and the salt-tolerant cultivar SR86 adopts a more flexible translationally adaptive strategy to cope with salt stress compared to the salt susceptible cultivar NB. The differences in translational dynamics between NB and SR86 may derive from their differing levels of ribosome stalling under salt stress.
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http://dx.doi.org/10.1186/s12864-021-07922-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359061PMC
August 2021

RiboNT: A Noise-Tolerant Predictor of Open Reading Frames from Ribosome-Protected Footprints.

Life (Basel) 2021 Jul 16;11(7). Epub 2021 Jul 16.

Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

Ribo-seq, also known as ribosome profiling, refers to the sequencing of ribosome-protected mRNA fragments (RPFs). This technique has greatly advanced our understanding of translation and facilitated the identification of novel open reading frames (ORFs) within untranslated regions or non-coding sequences as well as the identification of non-canonical start codons. However, the widespread application of Ribo-seq has been hindered because obtaining periodic RPFs requires a highly optimized protocol, which may be difficult to achieve, particularly in non-model organisms. Furthermore, the periodic RPFs are too short (28 nt) for accurate mapping to polyploid genomes, but longer RPFs are usually produced with a compromise in periodicity. Here we present RiboNT, a noise-tolerant ORF predictor that can utilize RPFs with poor periodicity. It evaluates RPF periodicity and automatically weighs the support from RPFs and codon usage before combining their contributions to identify translated ORFs. The results demonstrate the utility of RiboNT for identifying both long and small ORFs using RPFs with either good or poor periodicity. We implemented the pipeline on a dataset of RPFs with poor periodicity derived from membrane-bound polysomes of seedlings and identified several small ORFs (sORFs) evolutionarily conserved in diverse plant species. RiboNT should greatly broaden the application of Ribo-seq by minimizing the requirement of RPF quality and allowing the use of longer RPFs, which is critical for organisms with complex genomes because these RPFs can be more accurately mapped to the position from which they were derived.
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http://dx.doi.org/10.3390/life11070701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307163PMC
July 2021

Radically Enhanced Dual Recognition.

Angew Chem Int Ed Engl 2021 Aug 2. Epub 2021 Aug 2.

Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208, USA.

Complexation between a viologen radical cation (V ) and cyclobis(paraquat-p-phenylene) diradical dication (CBPQT ) has been investigated and utilized extensively in the construction of mechanically interlocked molecules (MIMs) and artificial molecular machines (AMMs). The selective recognition of a pair of V using radical-pairing interactions, however, remains a formidable challenge. Herein, we report the efficient encapsulation of two methyl viologen radical cations (MV ) in a size-matched bisradical dicationic host - namely, cyclobis(paraquat-2,6-naphthalene) , i.e., CBPQN . Central to this dual recognition process was the choice of 2,6-bismethylenenaphthalene linkers for incorporation into the bisradical dicationic host. They provide the space between the two bipyridinium radical cations in CBPQN suitable for binding two MV with relatively short (3.05-3.25 Å) radical-pairing distances. The size-matched bisradical dicationic host was found to exhibit highly selective and cooperative association with the two MV in MeCN at room temperature. The formation of the tetrakisradical tetracationic inclusion complex - namely, [(MV) ⊂CBPQN] - in MeCN was confirmed by VT H NMR, as well as by EPR spectroscopy. The solid-state superstructure of [(MV) ⊂CBPQN] reveals an uneven distribution of the binding distances (3.05, 3.24, 3.05 Å) between the three different V , suggesting that localization of the radical-pairing interactions has a strong influence on the packing of the two MV inside the bisradical dicationic host. Our findings constitute a rare example of binding two radical guests with high affinity and cooperativity using host-guest radical-pairing interactions. Moreover, they open up possibilities of harnessing the tetrakisradical tetracationic inclusion complex as a new, orthogonal and redox-switchable recognition motif for the construction of MIMs and AMMs.
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http://dx.doi.org/10.1002/anie.202109647DOI Listing
August 2021

The BTB/POZ domain protein GmBTB/POZ promotes the ubiquitination and degradation of the soybean AP2/ERF-like transcription factor GmAP2 to regulate the defense response to Phytophthora sojae.

J Exp Bot 2021 Aug 2. Epub 2021 Aug 2.

Soybean Research Institute of Northeast Agricultural University, Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin, China.

Phytophthora root and stem rot in soybean [Glycine max (L.) Merr.] is a destructive disease worldwide. Improving soybean resistance to the causal pathogen, Phytophthora sojae, is a major target for breeders; however, it remains largely unclear how the pathogen regulates the various affected signaling pathways in the host, which consist of complex networks including key transcription factors and their targets. We previously demonstrated that GmBTB/POZ enhances soybean resistance to P. sojae and associated defense response. Here, we report that GmBTB/POZ interacts with transcription factor GmAP2 and promotes the ubiquitination of GmAP2. The GmAP2-RNAi transgenic soybean hairy roots exhibited an enhanced resistance to P. sojae, whereas GmAP2-overexpressing hairy roots showed P. sojae hypersensitivity. Subsequently, GmWRKY33 was identified as a target of GmAP2, which represses its expression by directly binding to the GmWRKY33 promoter. GmWRKY33 acts as a positive regulator in the response of soybean to P. sojae. Additionally, the overexpression of GmBTB/POZ released the GmAP2-regulated suppression of GmWRKY33 expression in the GmAP2-OE soybean hairy roots and increased their resistance to P. sojae. Taken together, these results indicate a novel regulatory mechanism, the GmBTB/POZ-GmAP2 modulation of the P. sojae resistance response, which putatively regulates the downstream target gene GmWRKY33 in soybean.
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http://dx.doi.org/10.1093/jxb/erab363DOI Listing
August 2021

Pharmacokinetics and Pharmacodynamics of Novel Long-Acting Ropeginterferon Alfa-2b in Healthy Chinese Subjects.

Adv Ther 2021 09 30;38(9):4756-4770. Epub 2021 Jul 30.

Beijing Youan Hospital, Capital Medical University, Beijing, China.

Introduction: Ropeginterferon alfa-2b is a novel mono-pegylated human recombinant interferon (IFN) with the addition of N-terminal proline covalently attached by a 40-kDa polyethylene (peg) moiety. The present study aimed to evaluate the pharmacokinetics (PK), pharmacodynamics (PD) profiles and safety of the product in healthy Chinese.

Methods: Forty subjects were enrolled and treated with a single subcutaneous injection of either 180 mcg peg-IFN alfa-2a or 90, 180, and 270 mcg ropeginterferon alfa-2b.

Results: The mean T of ropeginterferon alfa-2b was 92-141 h and the elimination half-life was 78-129 h. Dose-related, non-proportional increase in ropeginterferon alfa-2b exposure was observed, which was higher than for peg-IFN alfa-2a. The PD parameters were similar between each dose level of ropeginterferon alfa-2b. The mean T of β-microglobulin ranged from 118 to 132 h after a single dose of ropeginterferon alfa-2b. The average E was 3 mcg/ml in all dose levels and the mean AUEC ranged from 1608 to 1775 h/mcg/ml. The TEAEs were comparable among each treatment group and no death nor drug-related SAE was reported.

Conclusion: Ropeginterferon alfa-2b is safe and well tolerated after a single subcutaneous injection up to 270 mcg in healthy Chinese.

Clinical Trial Registration: www.chinadrugtrials.org.cn , CTR20190451.
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http://dx.doi.org/10.1007/s12325-021-01863-yDOI Listing
September 2021

Neutrophil-to-lymphocyte ratio predicts the outcome of cerebral venous thrombosis.

Curr Neurovasc Res 2021 Jul 26. Epub 2021 Jul 26.

Department of Neurology, Zhengzhou University First Affiliated Hospital, Zhengzhou, Henan, China.

Background: Increasing evidences suggest that neutrophil-to- lymphocyte ratio (NLR) is an independent predictor of poor prognosis in patients with cardiovascular disease. However, the relationship between NLR and prognosis in patients with cerebral venous thrombosis (CVT) has not been studied.

Methods: Consecutive CVT patients from November 2011 through April 2019 were retrospectively identified. Poor outcome was defined as modified Rankin Scale (mRS) of 3-6. Multivariate regression analysis was conducted to assess the relationship between total and differential leukocyte counts, NLR and clinical outcome in CVT patients. The receiver operating characteristic (ROC) analysis was further performed to evaluate the ability to predict mortality and subgroup analysis was conducted to explore the potential interaction effects.

Results: A total of 360 CVT patients were included and the median duration of follow-up was 9.0 months. Multivariate logistic regression analysis suggested that NLR value, as a continuous variable, was significantly associated with a high risk of poor outcome (adjusted odds ratio [OR]=1.06, 95% confidence intervals [CI] 1.01-1.11, P = 0.013) and mortality (adjusted OR = 1.08; 95% CI, 1.03-1.14; P = 0.002). Compared with the total and differential leukocyte counts, the best discriminating variable to predict the risk of mortality was NLR and the area under the receiver operating curve was 0.81. The optimal cut-off value of NLR to predict mortality was 5.6 (sensitivity 84.2%, specificity 69.9%). Multivariate Cox regression analysis indicated that the mortality rate was significantly higher in patients with high NLR level group (>5.6) (adjust hazard ratio=5.65, 95% CI 2.33-12.73, P<0.001). There was no potential heterogeneity in the further subgroup analysis across age (above vs. below 45 years old), sex, history of infections and pregnancy/postpartum, presence of coma and intracerebral hemorrhage.

Conclusion: Elevated NLR value is associated with high risk of poor outcome in CVT patients.
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http://dx.doi.org/10.2174/1567202618666210726122310DOI Listing
July 2021

Correction to: Genetics and breeding for climate change in Orphan crops.

Theor Appl Genet 2021 Oct;134(10):3491-3492

The International Crops Research Institute for the Semi-Arid Tropics - Eastern and Southern Africa, Nairobi, Kenya.

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http://dx.doi.org/10.1007/s00122-021-03904-0DOI Listing
October 2021

MiR-141 attenuates sepsis-induced cardiomyopathy by targeting DAPK1.

Hum Exp Toxicol 2021 Jul 21:9603271211033768. Epub 2021 Jul 21.

Department of Endocrinology, 519688YanTaiShan Hospital, YanTai, China.

Objective: To discuss the possible effects of microRNA-141 (miR-141) in sepsis-induced cardiomyopathy (SIC) via targeting death-associated protein kinase 1 (DAPK1).

Methods: An SIC mouse model was constructed by abdominal injection of lipopolysaccharide (LPS) and divided into control, LPS, LPS + pre-miR-141, and LPS + anti-miR-141 groups. Hemodynamic indicators and heart function indexes of mice were detected. ELISA was used to determine the serum levels of inflammatory cytokines, while TUNEL staining to observe the apoptosis of myocardial cells of mice, as well as qRT-PCR and Western blotting to clarify the expression of miR-141 and DAPK1. Lastly, experiment was also conducted on the primary neonatal rat ventricular cardiomyocytes (NRVCMs) to validate the results.

Results: Mice in the LPS group, as compared to the control group, had lower left ventricular ejection fraction, left ventricular fractional shortening, left ventricular systolic pressure, and ±dp/dt, but a higher left ventricular end-diastolic pressure, while the serum expression of IL-1β, IL-6, TNF-α, and cTn-T was up-regulated evidently with the increased apoptotic index of myocardial tissues. However, miR-141 and Bcl-2/Bax were down-regulated with elevated DAPK1 and cleaved caspase-3. The above changes were ameliorated in mice from the LPS + pre-miR-141 group relative to the LPS group, while those in the LPS + anti-miR-141 group were further deteriorated. experiment showed that miR-141 overexpression could reduce the apoptosis of LPS-induced NRVCMs and the levels of inflammatory cytokines with the increased cell viability.

Conclusion: MiR-141 could decrease inflammatory response and reduce myocardial cell apoptosis by targeting DAPK1, thereby playing the promising protective role in SIC.
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http://dx.doi.org/10.1177/09603271211033768DOI Listing
July 2021

MiR-125a-5p silencing inhibits cerebral ischemia-induced injury through targeting IGFBP3.

Folia Neuropathol 2021 ;59(2):121-130

Department of Neurology, Qingdao Central Hospital, Qingdao, Shandong, China.

Dysregulated microRNAs (miRNAs) are crucial regulators of cerebrovascular conditions, including ischemic stroke. Circulating miR-125a-5p is associated with ischemic stroke and may have clinical utility as an early diagnostic biomarker. This study conducted a series of experiments that were designed to elucidate the regulatory action of miR-125a-5p in ischemic brain injury and its underlying mechanisms. The results of this study found that the expression of miR-125a-5p was increased in BV2 microglial cells under oxygen-glucose deprivation/reoxygenation (OGD/R) setting, as well as in rat brain tissue after middle cerebral artery occlusion (MCAO). OGD/R triggered BV2 microglial cell apoptosis, whereas downregulation of miRNA-125a-5p suppressed the apoptosis rate in OGD/R-induced BV2 microglial cells. Subsequently, insulin-like growth factor binding protein 3 (IGFBP3) is a molecular target of miR-125a-5p, and IGFBP3 knockdown reversed the effects of miR-125a-5p inhibitor on BV2 microglial cells in vitro. These data supported the fact that miR-125a-5p silencing protects against cerebral ischemia-induced injury by targeting IGFBP3.
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http://dx.doi.org/10.5114/fn.2021.107109DOI Listing
January 2021

Effective Regulation of ZnO Surface Facets for Enhanced Photoluminescence Properties Assisted by Zinc Quaternary Ammonium Salts.

ACS Omega 2021 Jul 29;6(27):17455-17463. Epub 2021 Jun 29.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

Novel ZnO twined-mushroom structures highly exposed in (001̅) planes were fabricated via a facile solvothermal synthesis with assistance of a zinc quaternary ammonium salt in the methanol-water solvent to show enhanced photoluminescence properties. A series of ZnO morphologies regulated with different surface facets were obtained in both MeOH-HO and EtOH MeOH-HO solvents respectively, tuning the proportion of alcohol. The self-aggregation mechanism was proposed based on the time-controlled experiment to evaluate the formation of twined-mushroom structures. The selective adsorptions of anions from zinc salt precursors determine the shape of subunits and direct the subunits, which act as building blocks to form the order aggregations.
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http://dx.doi.org/10.1021/acsomega.1c01841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280691PMC
July 2021

Microfragmented Adipose Tissue With Adjuvant Platelet-Rich Plasma Combination Therapy for Partial-Thickness Supraspinatus Tear.

Cureus 2021 Jun 10;13(6):e15583. Epub 2021 Jun 10.

H. Ben Taub Department of Physical Medicine and Rehabilitation, Baylor College of Medicine, Houston, USA.

A 50-year-old male presented with acute, sharp, right shoulder pain. Ultrasound of the right shoulder revealed a partial thickness tear of the supraspinatus. After conservative management failed to provide any relief, he was treated with microfragmented adipose tissue (MFAT) injection followed by platelet-rich plasma (PRP) at 14 weeks. At the 28-week follow-up, he showed significant improvement in pain and mobility with a resolution of the tear on ultrasound. While PRP has been shown to confer some protection against retears, very few studies have investigated the efficacy of MFAT use in rotator cuff pathology. In this case, we used a combination of MFAT and PRP to successfully treat a partial thickness supraspinatus tear. These agents may function in a synergistic manner, with MFAT providing a cell scaffold and PRP modulating the cellular environment to optimize healing. Further studies are needed to better understand the mechanism of this treatment modality in treating similar conditions.
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http://dx.doi.org/10.7759/cureus.15583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270056PMC
June 2021

Retraction Note to: Treatment with a brain-selective prodrug of 17β-estradiol improves cognitive function in Alzheimer's disease mice by regulating klf5-NF-κB pathway.

Naunyn Schmiedebergs Arch Pharmacol 2021 Sep;394(9):1989

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450002, China.

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http://dx.doi.org/10.1007/s00210-021-02121-2DOI Listing
September 2021

Real-world effectiveness of primary screening with high-risk human papillomavirus testing in the cervical cancer screening programme in China: a nationwide, population-based study.

BMC Med 2021 07 15;19(1):164. Epub 2021 Jul 15.

National Center for Women and Children's Health, Chinese Center for Disease Control and Prevention, 12 Dahuisi Road, Haidian District, Beijing, 100081, China.

Background: Randomized controlled trials have shown a higher sensitivity and longer negative predictive value of high-risk human papillomavirus (HPV) testing than cytology for cervical cancer screening; however, little is known about the effectiveness of HPV testing in middle-income countries. Understanding the characteristics of HPV testing may increase the priority of HPV testing in health policies. The study aims to evaluate the effectiveness of HPV testing in the national cervical cancer screening programme in China.

Methods: We performed a nationwide, population-based study using individual data from the national cervical cancer screening programme in rural China between 2015 and 2017. The analyses included 1,160,981 women aged 35-64 years who underwent cytology alone or high-risk HPV testing with cytology or genotyping triage. The main outcome was cervical intraepithelial neoplasia 2 or worse (CIN2+). We used multivariate logistic regressions and performed sensitivity analyses with propensity score matching to compare the screening positive, colposcopy referral, detection rate, and positive predictive value (PPV).

Results: The screening positive rates for HPV testing and cytology were 10.1% and 4.0%, respectively. The per protocol colposcopy referral rate of HPV testing was significantly lower than that of cytology (3.5% vs 4.0%), and this difference was mostly due to the low referral threshold of cytology (≥ASC-US). Overall, HPV testing detected more CIN2+ (5.5 vs. 4.4 per 1000, adjusted odds ratio [aOR]=1.18, 95% confidence interval 1.11-1.25) and had a higher PPV (13.8% vs 10.9%, aOR 1.29, 95% CI 1.21-1.37) than cytology. The colposcopy referrals of HPV testing in comparison to cytology differed by income status; it significantly increased in lower-middle-income areas (3.7% vs 3.1%, aOR 1.21, 95% CI 1.17-1.25) and significantly decreased in upper-middle-income areas (3.4% vs 4.9%, aOR 0.69, 95% CI 0.67-0.71). Sensitivity analyses demonstrated the reliability and robustness of the results.

Conclusions: The introduction of HPV testing could improve both the CIN2+ detection rate and efficiency of cervical cancer screening programme, supporting the introduction of primary screening with high-risk HPV testing in China. Further study is needed to investigate the long-term effect of this change.
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http://dx.doi.org/10.1186/s12916-021-02026-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281674PMC
July 2021

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs.

Anim Nutr 2021 Jun 27;7(2):376-383. Epub 2021 Feb 27.

Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha 410125, China.

The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait, meat quality and lipid metabolism of finishing Huan Jiang mini-pigs. Betaine called trimethylglycine is a methyl derivative of glycine, but few researches were conducted to compare the impact of dietary betaine and glycine on pigs. One hundred and forty-four Huan Jiang mini-pigs (body weight = 10.55 ± 0.15 kg; 70 d) were randomly divided to 3 treatment groups (basal diet, glycine or betaine). Results indicated that dietary betaine increased the average daily gain (ADG) and final weight ( < 0.05). Dietary glycine or betaine markedly reduced average backfat thickness ( < 0.05) and heightened lean percentage ( < 0.01) compared to the control group. Moreover, in comparison with the control group, betaine significantly improved the redness (a∗) and tenderness (shear force) of the longissimus dorsi (LD) muscle ( < 0.05), whereas glycine only raised the value of a∗ of the LD muscle ( < 0.05). These results showed that diet supplemented with 0.25% betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs, and the effect of betaine was superior to that of glycine.
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http://dx.doi.org/10.1016/j.aninu.2020.08.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245815PMC
June 2021

The characteristics and risk factors of healthcare-seeking men with lower urinary tract symptoms in China: Initial report from the POInT group.

Neurourol Urodyn 2021 Sep 12;40(7):1740-1753. Epub 2021 Jul 12.

Bristol Urological Institute, Southmead Hospital, Bristol, UK.

Aims: To investigate the clinical characteristics of health care-seeking men presenting with lower urinary tract symptoms (LUTS) in China and to reveal risk factors for symptom severity.

Methods: This multicenter, hospital-based, cross-sectional study recruited 1477 eligible male subjects, who were at least 45 years, seeking health care at 9 participating hospitals across the mainland China. The general medical information and subjective symptoms were recorded, followed by the measurement of prostate volume, urodynamic indices, and laboratory tests for kidney function, plus glucose/lipid metabolism. Univariate and multivariate linear regression were employed for the detection of risk factors for symptom severity.

Results: The proportion of mild, moderate, and severe LUTS was 14.6%, 32.6%, and 52.8%, respectively, with 62.2% reporting the triple combination of storage, voiding, and postmicturition symptoms. Median prostate volume was 44.6 ml, and 71.1% were experiencing comorbidities. Thirteen independent risk factors for LUTS severity were identified, namely, nocturnal voiding episodes and the presence of straining and weak steam; the triple combination of symptom subtypes; general and nocturia quality of life; Q and bladder outlet obstruction index; and numbers of comorbidities, hypertension, estimated glomerular filtration rate, and cholesterol and glycosylated hemoglobin.

Conclusions: The majority of health care-seeking LUTS men present with moderate-to-severe and overlapping symptoms, with a high prevalence of both lower urinary tract dysfunction and systemic comorbidities. The evidence from both urological and nonurological independent risk factors demonstrate the multifactorial nature of LUTS, for which a multidisciplinary management is essential.
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http://dx.doi.org/10.1002/nau.24737DOI Listing
September 2021

Serum Immunoglobulin G level and Neutrophils to Lymphocytes Ratio Associated with the Prognosis of Neuromyelitis Optica Spectrum Disorder.

Curr Neurovasc Res 2021 Jul 1. Epub 2021 Jul 1.

Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Objective: To investigate the factors related to the prognosis of neuromyelitis optica spectrum disorder (NMOSD) in cerebrospinal fluid and peripheral blood examination.

Methods: In this study, we collected 111 patients who were admitted to the First Affiliated Hospital of Zhengzhou University between January 2016 and January 2018 and diagnosed with NMOSD. The patients were divided into the relapse group (n=48) and remission group (n=67). Before treatment, all the patients underwent a routine cerebrospinal fluid (CSF) and peripheral blood test on the second morning of admission. The association between laboratory data and disease prognosis was evaluated.

Results: The immunoglobulin G (IgG) level in the serum showed a strong correlation with the relapse of patients, especially in the aquaporin-4-Antibody (AQP4-Ab) positive group (p<0.01). A high level of serum IgG concentration was associated with the relapse of NMOSD, especially in the anti-AQP4 positive group. The area under the receiver operating characteristic (ROC) curve of serum IgG level was 0.888 (p<0.001, 95%CI: 0.808-0.968). The ratio of neutrophils to lymphocytes (NLR) was associated with the disability degree of NMOSD patients in 3 years. The NLR value was a linear correlation with final Expanded Disability Status Scale (EDSS) scores. Patients with a high level of NLR value presented an increased degree of disability in the following three years (R2=0.053, p=0.015).

Conclusion: The serum IgG level and NLR of first-attack patients were correlated with the prognosis of NMOSD.
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http://dx.doi.org/10.2174/1567202618666210702113526DOI Listing
July 2021

Sulfur-zinc modified kaolin/steel slag: A particle electrode that efficiently degrades norfloxacin in a neutral/alkaline environment.

Chemosphere 2021 Jun 28;284:131328. Epub 2021 Jun 28.

College of Earth and Environmental Sciences, Key Lab of Environmental Pollution Predict & Control, Lanzhou University, Lanzhou, 730000, PR China.

In this work, sulfur and zinc were used to modify the steel slag/kaolin particle electrodes. Sulfur-zinc modified kaolin/steel slag particle electrodes (S-Zn-KSPEs) was successfully prepared. In a wide pH range (pH 3-10), S-Zn-KSPEs could efficiently degrade norfloxacin at low voltage (4 V) within 90 min. The removal rate of NOR by S-Zn-KSPEs was about 100% in acidic environment, more than 90% in neutral environment, and more than 80% in alkaline environment. And S-Zn-KSPEs could also efficiently degrade methylene blue, diuron, levofloxacin and other refractory pollutants under neutral conditions. S-Zn-KSPEs showed good stability and recyclability, and could maintain high catalytic activity after 8 cycles in a neutral or alkaline environment. The possible degradation mechanism and the degradation pathway of norfloxacin are proposed. In addition, S-Zn-KSPEs also showed a higher treatment effect in the treatment of actual surface water bodies. And S-Zn-KSPEs had a strong acid-base buffering capacity, which could avoid some pretreatment measures of wastewater in practical applications.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131328DOI Listing
June 2021

Corrigendum to "miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation" [Chem. Biol. Interact. 277 (2017) 33-42].

Chem Biol Interact 2021 Aug 29;345:109556. Epub 2021 Jun 29.

Department of Pathology and Forensics, Dalian Medical University, No.9 West Section, Lvshun South Road, Dalian, 116044, China; Teaching Laboratory of Morphology, Dalian Medical University, No.9 West Section, Lvshun South Road, Dalian, 116044, China. Electronic address:

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http://dx.doi.org/10.1016/j.cbi.2021.109556DOI Listing
August 2021

UV-fluorescence probe for detection Ni with colorimetric/spectral dual-mode analysis method and its practical application.

Bioorg Chem 2021 Sep 18;114:105103. Epub 2021 Jun 18.

Joint Laboratory of Advanced Biomedical Materials (NFU-UGent), Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing 210037, PR China. Electronic address:

Fluorescence probe combines with fluorescence imaging technology has become the most powerful analytical method with their great advantages of high sensitivity and selectivity and real-time monitoring. Ni is widely distributed in food, environment and living animals, thereof, it is of great significance for detection Ni with high selectivity. Herein, a simple strategy is proposed to design and synthesiz a small molecule fluorescent probe Y1 by using "one-pot" method. The spectroscopic behaviors including UV-Vis absorption and fluorescence emission spectrum have been used to verify the feasibility of probe towards Ni in water/EtOH (v/v = 2:8) mixtures under neutral condition. As expected, Y1 offers high selectivity and sensitivity for detection Ni in aqueous solution with a good linear relationship and low detection limit within Ni concentration variation from 0 to 13 μM (DOL = 0.0038 μM, R = 0.9983). It is remarkable that Y1 can be applied for real-time visualization Ni change in sprouted potato and zebrafish with great photo-stability, highlighting that the practicability and feasibility of Y1 to detect and monitor Ni in the field of food industry and biomedical field.
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http://dx.doi.org/10.1016/j.bioorg.2021.105103DOI Listing
September 2021

Prediction of arrhythmia after intervention in children with atrial septal defect based on random forest.

BMC Pediatr 2021 06 16;21(1):280. Epub 2021 Jun 16.

Qingdao Women and Children's Hospital, Qingdao University, 266034, Qingdao, China.

Background: Using random forest to predict arrhythmia after intervention in children with atrial septal defect.

Methods: We constructed a prediction model of complications after interventional closure for children with atrial septal defect. The model was based on random forest, and it solved the need for postoperative arrhythmia risk prediction and assisted clinicians and patients' families to make preoperative decisions.

Results: Available risk prediction models provided patients with specific risk factor assessments, we used Synthetic Minority Oversampling Technique algorithm and random forest machine learning to propose a prediction model, and got a prediction accuracy of 94.65 % and an Area Under Curve value of 0.8956.

Conclusions: Our study was based on the model constructed by random forest, which can effectively predict the complications of arrhythmia after interventional closure in children with atrial septal defect.
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http://dx.doi.org/10.1186/s12887-021-02744-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207618PMC
June 2021

Roles of amino acid derivatives in the regulation of obesity.

Food Funct 2021 Jul 8;12(14):6214-6225. Epub 2021 Jun 8.

CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

Obesity is an issue of great concern to people all over the world. It is accompanied by serious complications, leading to reduced quality of life and higher morbidity and mortality. Over the past few years, there has been an explosion in knowledge about the roles of potential therapeutic agents in obesity management. Among them, amino acid (AA) derivatives, such as taurine, glutathione (GSH), betaine, α-ketoglutarate (AKG), β-aminoisobutyric acid (BAIBA), and β-hydroxy-β-methylbutyrate (HMB), have recently gained popularity due to their beneficial effects on the promotion of weight loss and improvement in the lipid profile. The mechanisms of action of these derivatives mainly include inhibiting adipogenesis, increasing lipolysis, promoting brown/beige adipose tissue (BAT) development, and improving glucose metabolism. Therefore, this review summarizes these AA derivatives and the possible mechanisms responsible for their anti-obesity effects. Based on the current findings, these AA derivatives could be potential therapeutic agents for obesity and its related metabolic diseases.
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http://dx.doi.org/10.1039/d1fo00780gDOI Listing
July 2021
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