Publications by authors named "Bo Pang"

412 Publications

New Variant of Vibrio parahaemolyticus, Sequence Type 3, Serotype O10:K4, China, 2020.

Emerg Infect Dis 2022 Jun;28(6):1261-1264

In 2020, a new serotype of Vibrio parahaemolyticus O10:K4 emerged and caused several outbreaks and sporadic cases in Guangxi, China. Phylogenetic analysis indicated that those strains are new variants of the sequence type 3 pandemic clone. The new serotype may become dominant, warranting enhanced investigations and surveillance.
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http://dx.doi.org/10.3201/eid2806.211871DOI Listing
June 2022

Clinical Value of Three-Dimensional Transvaginal Ultrasound in Diagnosis of Endometrial Receptivity and Ovarian Function in Patients with Infertility.

Comput Math Methods Med 2022 11;2022:8438131. Epub 2022 May 11.

Ultrasonics, Yi Jishan Hospital, Wannan Medical College, Wuhu 241000, China.

Objective: A case-control study was conducted to explore the clinical value of three-dimensional transvaginal ultrasound in the diagnosis of endometrial receptivity (ER) and ovarian function in patients with infertility.

Methods: A total of 308 infertile women treated in our hospital from March 2020 to June 2021 were enrolled as the observation group, and another 300 women of childbearing age who underwent physical examination in the same period were enrolled as the control group. The clinical value of three-dimensional transvaginal ultrasound in ER in patients with infertility was analyzed by comparing the classification of endometrial and subendometrial blood perfusion, endometrial AUC value and Pi value, and subendometrial AUC value and Pi value. According to the number of oocytes obtained, the patients were assigned into the normal response group (182 cases, ≥5 oocytes) and the low response group (126 cases, <5 oocytes). The levels of some hormones, such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and FSH/LH, were measured. Transvaginal ultrasonography was performed to detect ovarian volume (OV), antral follicle count (AFC), and peak flow rate of the ovarian interstitial artery (PSV). The peak of end-diastolic flow velocity (EDV) and other indexes were analyzed. The correlation between FSH level, FSH/LH, and ultrasound indexes was analyzed, and the ROC curve was established to analyze the value of transvaginal Doppler ultrasound in evaluating ovarian reserve function and predicting ovulation.

Results: There were significant differences in late proliferation type I and type III, ovulatory type II and type III ( < 0.05). There exhibited no significant difference in late proliferation type II, ovulation stage type I, and implantation window stage type I, type II, and type III ( > 0.05). Regarding the endometrial AUC and Pi values, the endometrial AUC and Pi values in the observation group were lower compared to the control group during late proliferation and ovulation ( < 0.05). There exhibited no significant difference in AUC and Pi ( > 0.05). Regarding the subintimal AUC and Pi values, the subintimal AUC and Pi values in the observation group were higher compared to the control group during late proliferation and ovulation ( < 0.05). There exhibited no significant difference in AUC and Pi during the implantation window ( > 0.05). There exhibited no significant difference in menarche age, age, body mass index, and menstrual cycle between the normal response group and the low response group ( > 0.05). The levels of EDV, OV, AFC, and PSV in the normal response group were higher compared to the low response group ( < 0.01). Compared with the low response group, the levels of FSH and FSH/LH in the normal response group were lower, but the levels of LH and E2 in the normal response group were higher ( < 0.05). The results of correlation analysis of FSH, FSH/LH, and ultrasound parameters between the normal response group and the low response group indicated that FSH was negatively correlated with E2, EDV, OV, AFC, and PSV in 308 infertile women ( = -0.817, -0.846, -0.707, -0.845, -0.911, < 0.01), but it was positively correlated with FSH/LH ( = 0.714, < 0.01). The ultrasound parameters of ovarian reserve function in the normal response group and the low response group were compared with the indexes that predicted ovulation. The results of ROC curve analysis indicated that the cutoff values of EDV, OV, AFC, and PSV were 4.141, 3.726, 4.106, and 13.944, respectively, the specificity of each index was higher than 90.00%, and the sensitivity was higher than 80.00% except PSV.

Conclusion: Transvaginal ultrasound can not only accurately evaluate the ER of infertile women but also directly observe follicular development and monitor ovulation, which is of high value in evaluating ovarian reserve function and predicting ovulation.
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http://dx.doi.org/10.1155/2022/8438131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117042PMC
May 2022

Cholera Caused by a New Clone of Serogroup O1 - Beijing Municipality, China, June 2021.

China CDC Wkly 2022 Jan;4(2):31-32

Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing, China.

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http://dx.doi.org/10.46234/ccdcw2021.279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796729PMC
January 2022

Predicting risk of overweight or obesity in Chinese preschool-aged children using artificial intelligence techniques.

Endocrine 2022 May 18. Epub 2022 May 18.

Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China.

Objectives: We adopted the machine-learning algorithms and deep-learning sequential model to determine and optimize most important factors for overweight and obesity in Chinese preschool-aged children.

Methods: This is a cross-sectional survey conducted in 2020 at Beijing and Tangshan. Using a stratified cluster random sampling strategy, children aged 3-6 years were enrolled. Data were analyzed using the PyCharm and Python.

Results: A total of 9478 children were eligible for inclusion, including 1250 children with overweight or obesity. All children were randomly divided into the training group and testing group at a 6:4 ratio. After comparison, support vector machine (SVM) outperformed the other algorithms (accuracy: 0.9457), followed by gradient boosting machine (GBM) (accuracy: 0.9454). As reflected by other 4 performance indexes, GBM had the highest F1 score (0.7748), followed by SVM with F1 score at 0.7731. After importance ranking, the top 5 factors seemed sufficient to obtain descent performance under GBM algorithm, including age, eating speed, number of relatives with obesity, sweet drinking, and paternal education. The performance of the top 5 factors was reinforced by the deep-learning sequential model.

Conclusions: We have identified 5 important factors that can be fed to GBM algorithm to better differentiate children with overweight or obesity from the general children, with decent prediction performance.
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http://dx.doi.org/10.1007/s12020-022-03072-1DOI Listing
May 2022

Fabrication of [email protected] Polyphenols/Cellulose as Active Food Packaging and Its Enhanced Antibacterial Activity.

Int J Mol Sci 2022 May 7;23(9). Epub 2022 May 7.

Key Laboratory of Functional Inorganic Material Chemistry (Ministry of Education), School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080, China.

To investigate the efficient use of bioresources and bioproducts, plant polyphenol (PPL) was extracted from larch bark and further applied to prepare [email protected]/Cel with cellulose to examine its potential as an active package material. The structure and morphology were fully characterized by XRD, SEM, FTIR, XPS and Raman spectra. It was found that PPL is able to cover ZnO and form a coating layer. In addition, PPL cross-links with cellulose and makes ZnO distribute evenly on the cellulose fibers. Coating with PPL creates a pinecone-like morphology in ZnO, which is constructed by subunits of 50 nm ZnO slices. The interactions among ZnO, PPL and cellulose have been attributed to hydrogen bonding, which plays an important role in guiding the formation of composites. The antibacterial properties against Gram-negative () and Gram-positive () were tested by the inhibition zone method. Our composite [email protected]/Cel has superior antibacterial activity compared to ZnO/Cel. The antibacterial mechanism has also been elaborated on. The low cost, simple preparation method and good performance of [email protected]/Cel suggest the potential for it to be applied as active food packaging.
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http://dx.doi.org/10.3390/ijms23095218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104473PMC
May 2022

Rapid qualitative and quantitative detection of Salmonella typhimurium using a single-step dual photometric/fluorometric assay.

Mikrochim Acta 2022 05 11;189(6):218. Epub 2022 May 11.

School of Public Health, Jilin University, Changchun, 130021, People's Republic of China.

A dual-signal photometric/fluorometric assay was established for rapid, qualitative, and quantitative detection of Salmonella typhimurium (S. typhimurium). This method was composed of two parts: (1) a single-step photometric (SSC) assay containing gold nanoparticles (AuNPs), poly-diallyldimethylammonium chloride (PDDA), and S. typhimurium-specific aptamer, and (2) a fluorescence (FL) assay containing carboxyl-modified CdSe/ZnS quantum dots (QDs-). Users just need to drop samples contaminated with S. typhimurium into SSC assay; the apparent color change from red to blue can be observed in a short time (20 min). A smartphone app was developed to read the semiquantitative result. By subsequently adding one drop of FL assay into the reaction mixture, the generated fluorescence intensity reflected the concentration of S. typhimurium. The naked eye limit of detection (LOD) and fluorescent LOD were 10 cfu/mL and 10 cfu/mL, respectively. This method exhibited good selectivity. The reliability and practicability were verified by testing contaminated food, drinking water, and pets' urine.
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http://dx.doi.org/10.1007/s00604-022-05312-7DOI Listing
May 2022

[Clinical trials and evaluation of Chinese patent medicine for stroke].

Zhongguo Zhong Yao Za Zhi 2022 May;47(9):2330-2337

Centre for Evidence-based Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.

To systematically collect and analyze clinical randomized controlled trial(RCT) of Chinese patent medicine treatment for stroke in 2020, in order to provide basic information for clinical decision-making and related research. On the basis of the collection in the Traditional Chinese Medicine(TCM) Clinical Evidence Database System(EVDS), CNKI, Wanfang, SinoMed, Cochrane Library, PubMed, EMbase were searched for RCTs of Chinese patent medicine for stroke in 2020. The publication, sample size, intervention and control measures, course of treatment, outcome indicators, methodological quality and other contents were statistically analyzed.A total of 68 RCTs studies on Chinese patent medicine for stroke were included in 2020, of which 29(42.60%) were RCTs with sample size>100 cases. A total of 41 kinds of proprietary Chinese medicines were involved, including 23 kinds of oral proprietary Chinese medicines and 18 kinds of injections. A total of 18 intervention/control cases were included in RCTs, and 19 cases(Chinese patent medicine+Western medicine vs Western medicine) were applied in RCTs, accounting for 27.90%. The duration of treatment was reported in 91.18% of the studies, and the intervention duration was 8-14 days in 50.00% of the studies. Evaluation indexes were widely used, among which physical and chemical testing indexes(49.36%) were the most widely used. According to the methodological quality evaluation, the overall methodological quality of the study is not high, especially the implementation of the allocation hidden, blind method is not accurate, and the registration, ethics and other links are often missing. In conclusion, 2020 implementation of proprietary Chinese medicine in the treatment of stroke research methodology problems is outstanding, the similar function scale large range of optional, no specification selection criteria, reliability and practicability of the impact study, need to further standardize the proprietary Chinese medicine in the treatment of stroke study design, implementation and quality control, and highlight the value of proprietary Chinese medicine in the treatment of stroke and improve the quality of the evidence.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220222.503DOI Listing
May 2022

[Clinical evidence analysis report of Chinese patent medicine in 2020].

Zhongguo Zhong Yao Za Zhi 2022 May;47(9):2315-2321

Center of Evidence-based Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.

The present study collected, collated, analyzed, and evaluated randomized controlled trial(RCT) of Chinese patent medicine published in Chinese and English journals in 2020, and summarized clinical evidence of Chinese patent medicine in stages, providing references for follow-up clinical research and evidence transformation and application. On the basis of the collection in the Traditional Chinese Medicine(TCM) Clinical Evidence Database System(EVDS), CNKI, Wanfang, SinoMed, Cochrane Library, PubMed, and EMbase were searched for RCTs of Chinese patent medicine published in 2020, and their research characteristics and methodological quality were analyzed and evaluated. A total of 1 285 research papers on Chinese patent medicine(1 257 in Chinese/28 in English) were included, involving 146 054 patients and 639 Chinese patent medicines, including 526 oral drugs, 68 injections, and 45 external drugs. A total of 412 diseases in 23 types were involved, which were dominated by circulatory system diseases and respiratory system diseases, specifically, cerebral infarction and angina pectoris. The sample size ranged from 20 cases to 2 673 cases, and 57.67% of RCTs had samples sizes less than 100. Single-center trials were the main ones, and multi-center trials only accounted for 4.75%(n=61). In terms of methodological quality, 52.91% of the RCTs had unclear descriptions or incorrect application of randomization methods, and the implementation of allocation concealment and blinding methods has not been paid much attention. In conclusion, compared with the conditions in 2019, the number of RCTs published in 2020 has decreased, and the research interest in respiratory diseases has increased, while the quality control in the process of research design and implementation has not been improved. Therefore, it is necessary to strengthen the methodological training of researchers and promote the output of high-quality research evidence.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220209.502DOI Listing
May 2022

Seizure prophylaxis following aneurysmal subarachnoid haemorrhage (SPSAH): study protocol for a multicentre randomised placebo-controlled trial of short-term sodium valproate prophylaxis in patients with acute subarachnoid haemorrhage.

BMJ Open 2022 May 4;12(5):e057917. Epub 2022 May 4.

Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Introduction: Seizures are a common complication that leads to neurological deficits and affects outcomes after aneurysmal subarachnoid haemorrhage (aSAH). However, whether to use prophylactic anticonvulsants in patients with aSAH remains controversial. Our study aims to determine whether short-term (7 days) sodium valproate could prevent seizure occurrence and improve neurological function in patients with SAH caused by anterior circulation aneurysm rupture and treated with clipping.

Methods And Analysis: In this multicentre randomised evaluator-blind placebo-controlled trial, 182 eligible patients with good-grade aSAH planned for surgical clipping will be enrolled from four neurosurgical centres in China. In addition to standard care, patients will be randomly assigned to receive sodium valproate 20 mg/kg daily or matching placebo. After aneurysmal clipping, patients will be followed up at discharge, 90 days and 180 days. The primary outcomes are the incidence of early and late seizures. The secondary outcomes include aSAH-related complications, sodium valproate-related adverse effects, modified Rankin Scale (mRS) (on discharge, at 90 days, 180 days), rate of good outcome (defined as mRS 0-2), all-cause death (at 90 days, 180 days) and Montreal Cognitive Assessment score (at 180 days). All analyses are by intention-to-treat.

Ethics And Dissemination: This study will be conducted according to the principles of Declaration of Helsinki and good clinical practice guidelines. This trial involves human participants and has been approved by the ethics committee of West China Hospital. Informed consent will be achieved from each included patient and/or their legally authorised representative. Preliminary and final results from this study will be disseminated through manuscript publishing and international congresses presentations. Any protocol amendments will be approved by the ethics committee of West China Hospital and subsequently updated on ChiCTR.

Trial Registration Number: ChiCTR.org identifier: ChiCTR2100050161.
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http://dx.doi.org/10.1136/bmjopen-2021-057917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073395PMC
May 2022

The relationship between serum vitamin K concentration and coronary artery calcification in middle-aged and elderly people.

Clin Chim Acta 2022 May 2;531:325-330. Epub 2022 May 2.

Clinical Laboratory, Guang' anmen Hospital, China academy of Chinese medical sciences, China. Electronic address:

Background: Vitamin K is involved in the formation of coronary artery calcification which is an independent predictor of coronary heart disease. This study aims to explore the association between coronary artery calcification score and serum concentrations of vitamin K1, menaquinone-4 (MK-4) and menaquinone-7 (MK-7) in middle-aged and elderly Chinese population.

Methods: A total of 116 patients who underwent CT coronary angiography were consecutively enrolled. Serum concentrations of vitamin K1, MK-4 and MK-7 were determined by high performance liquid chromatography tandem mass spectrometry. The relationships between coronary artery calcification score and serum vitamin K concentrations were analyzed.

Results: Significantly lower serum vitamin K1 concentration was found in the patients with CACS > 400, comparing with the other CACS categories, respectively. Log (CACS + 1) was significantly higher in MK-4 < 0.05 ng/ml group compared with MK-4 ≥ 0.05 ng/ml group [2.03(0.21, 2.58) vs 1.31(0.00, 2.19), P < 0.05]. In subjects with established coronary calcification (defined as CACS > 10), vitamin K1 was found to be an independent factor contributing to higher CACS (r = -0.288, P = 0.013).

Conclusions: In this retrospective analysis, serum vitamin K1 and MK-4 concentrations were significantly lower in middle-aged and elderly cohorts with increasing calcification scores. The significant effect of vitamin K1 on CACS was only found in individuals who already had calcification. Whether the detection of circulating vitamin K in patients with preexisting coronary calcification could guide vitamin K supplementation needs further exploration.
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http://dx.doi.org/10.1016/j.cca.2022.04.1001DOI Listing
May 2022

Transcriptome analysis provides new insights into cold adaptation of corsac fox ().

Ecol Evol 2022 Apr 19;12(4):e8866. Epub 2022 Apr 19.

College of Life Science Qufu Normal University Qufu China.

are widely distributed throughout the world and have undergone drastic physiological and phenotypic changes in response to their environment. However, little is known about the underlying genetic causes of these traits, especially . In this study, RNA-Seq was used to obtain a comprehensive dataset for multiple pooled tissues of corsac fox, and selection analysis of orthologous genes was performed to identify the genes that may be influenced by the low-temperature environment. More than 6.32 Gb clean reads were obtained and assembled into a total of 173,353 unigenes with an average length of 557 bp for corsac fox. Selective pressure analysis showed that 16 positively selected genes (PSGs) were identified in corsac fox, red fox, and arctic fox. Enrichment analysis of PSGs showed that the  gene was enriched in several pathways related to the low-temperature response and might play a key role in response to environmental stimuli of foxes. In addition, several positively selected genes were related to DNA damage repair ( and ), innate immunity ( and ), and the respiratory chain (), and these positively selected genes might play a role in adaptation to harsh wild fox environments. The results of common orthologous gene analysis showed that gene flow or convergent evolution might be an important factor in promoting regional differentiation of foxes. Our study provides a valuable transcriptomic resource for the evolutionary history of the corsac fox and the adaptations to the extreme environments.
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http://dx.doi.org/10.1002/ece3.8866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019142PMC
April 2022

Visual Identification and Serotyping of Toxigenic Serogroups O1 and O139 With CARID.

Front Cell Infect Microbiol 2022 31;12:863435. Epub 2022 Mar 31.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

There is a growing demand for rapid, sensitive, field-deployable nucleic acid tests for cholera, which usually occurs in rural areas. In this study, we developed a Cas12a-assisted rapid isothermal detection (CARID) system for the detection of toxigenic serogroups O1 and O139 by combining recombinase-aided amplification and CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins). The results can be determined by fluorescence signal and visualized by lateral flow dipstick. We identified 154 V strains and 129 strains of other intestinal diarrheagenic bacteria with a 100% coincidence rate. The limit of detection of CARID was 20 copies/reaction of genomic DNA, which is comparable to that of polymerase chain reaction (PCR) and qPCR. Multiple-CARID was also established for efficiency and economic considerations with an acceptable decrease in sensitivity. Simulated sample tests showed that CARID is suitable for complex samples. In conclusion, CARID is a rapid, sensitive, economically efficient, and portable method for the detection of , which makes it suitable for field responses to cholera.
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http://dx.doi.org/10.3389/fcimb.2022.863435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008587PMC
April 2022

Phylogenetic Analysis of Serogroup O5 that Caused Successive Cholera Outbreaks - Guangdong Province, China, 2020-2021.

China CDC Wkly 2022 Mar;4(12):238-241

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Introduction: Gastroenteritis caused by non-O1/non-O139 exhibited an increasing trend in recent years in China. Whole genome sequence (WGS) data could play an important role both in the identification of the outbreaks and in the determination of the serogroup. Here, we present the employment of WGS data in the investigation of two outbreaks caused by non-O1/non-O139 in Guangdong, China, 2020-2021.

Methods: We obtained the whole genome sequence of 66 strains isolated in two outbreaks with next generation sequencing technology. We retrieved the publicly available WGS data of non-O1/non-O139 from public database. We used a pipeline integrated in China Pathogen Identification Net (PIN) to complete the phylogenetic analysis.

Results: Two outbreaks caused by non-O1/non-O139 were identified using WGS data. These strains were determined as serogroup O5. Type 3 and 6 secretion systems were detected in these serogroup O5 strains. These serogroup O5 strains belonged to sequence type (ST) 88.

Conclusions: Our analysis indicated the risk of non-O1/non-O139 leading to outbreaks of diarrheal diseases. The application of genomic data played an important role in the identification of the serogroup of non-O1/non-O139 in the lack of antiserum, which gave an example of the application of genome data in disease surveillance and public health emergency response.
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http://dx.doi.org/10.46234/ccdcw2022.061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9005492PMC
March 2022

Cellulose Nanopaper: Fabrication, Functionalization, and Applications.

Nanomicro Lett 2022 Apr 13;14(1):104. Epub 2022 Apr 13.

Sustainable Materials and Chemistry, Department of Wood Technology and Wood-Based Composites, University of Göttingen, 37077, Göttingen, Germany.

Cellulose nanopaper has shown great potential in diverse fields including optoelectronic devices, food packaging, biomedical application, and so forth, owing to their various advantages such as good flexibility, tunable light transmittance, high thermal stability, low thermal expansion coefficient, and superior mechanical properties. Herein, recent progress on the fabrication and applications of cellulose nanopaper is summarized and discussed based on the analyses of the latest studies. We begin with a brief introduction of the three types of nanocellulose: cellulose nanocrystals, cellulose nanofibrils and bacterial cellulose, recapitulating their differences in preparation and properties. Then, the main preparation methods of cellulose nanopaper including filtration method and casting method as well as the newly developed technology are systematically elaborated and compared. Furthermore, the advanced applications of cellulose nanopaper including energy storage, electronic devices, water treatment, and high-performance packaging materials were highlighted. Finally, the prospects and ongoing challenges of cellulose nanopaper were summarized.
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http://dx.doi.org/10.1007/s40820-022-00849-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008119PMC
April 2022

Progress in pretreatment of methadone: an update since 2015.

Authors:
Bo Pang Yinru Jiang

Prep Biochem Biotechnol 2022 Apr 2:1-11. Epub 2022 Apr 2.

China Medical University-The Queen's University of Belfast Joint College, China Medical University, Shen Yang, China.

Methadone, a µ-opioid receptor agonist, is widely used in pain-relieving and treating opioid dependence. If not strictly controlled, as an opioid substitute, it can lead to abuse and cause more severe withdrawal responses than heroin. Also, overdose or abuse of this drug in clinical use may provide severe side effects such as apnea, circulatory collapse, cardiac arrest, and even death. For these reasons, simple, rapid, and efficient methods have been developed for the pretreatment of methadone. This review presents a comprehensive conclusion of the pretreatment methods used for methadone in various sample matrices, focusing on the developments since 2015. Traditionally used pretreatment methods like solid-phase extraction and liquid-liquid extraction are discussed and newly developed methods like solid-phase microextraction and liquid-liquid microextraction along with new materials applied are focused.
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http://dx.doi.org/10.1080/10826068.2022.2056900DOI Listing
April 2022

Acquisition pattern of nitrogen by microorganisms and plants affected by gravel mulch in a semiarid Tibetan grassland.

Sci Total Environ 2022 Jul 18;830:154635. Epub 2022 Mar 18.

Key Laboratory of Mountain Environment Evolution and Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China; Xainza Alpine Steppe and Wetland Ecosystem Observation Station, Xainza 853100, China. Electronic address:

As an important coarse inorganic fraction of soil, gravel may regulate the effects of the interaction between above- and belowground communities and affect the relationship between microorganisms and plants in alpine ecosystems. However, comparatively little is known about the effects of gravel on the acquisition pattern of nitrogen (N) by microorganisms and plants in alpine ecosystems. In this study, a N-labelling experiment was conducted to investigate the acquisition pattern of organic (N-glycine) and inorganic N (N-NO and N-NH) by microorganisms and plants under three particle sizes of gravel mulch (fine: 2-10 mm, medium: 10-20 mm, coarse: 20-40 mm) on a semiarid Tibetan grassland. Gravel mulch significantly improved the N recovery of Stipa purpurea, but had no significant impacts on A. nanschanica. Therefore, gravel mulch decreased the ratio of microbial biomass N recovery to plant biomass N recovery for S. purpurea, but caused little effect on the state of N competition between plants and soil microbes for A. nanschanica. The N absorption preference of plants from both species shifted from an individual preference for N-NO in the natural (i.e., control) microplots to a common preference for N-NOand N-NH in the fine- and medium-sized gravel mulch microplots, while there were no significant differences in microbial N recovery between N-NO and N-NH across all treatments. The results helped to improve the understanding of the acquisition pattern of N by microorganisms and plants under the influence of gravel mulch in alpine ecosystems, and provide theoretical support for revegetation in alpine ecosystems in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2022.154635DOI Listing
July 2022

Factors Associated With Severe Fever With Thrombocytopenia Syndrome in Endemic Areas of China.

Front Public Health 2022 24;10:844220. Epub 2022 Feb 24.

Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, China.

Objective: To explore the influence of climatic, environmental and socioeconomic factors on SFTS occurrence in Shandong Province, China.

Methods: We used generalized additive model to estimate the association between SFTS cases and climatic factors, environmental factors and socioeconomic factors, including annual average temperature, precipitation, land cover, normalized difference vegetation index, altitude, population density, meat production, milk production, and gross domestic product (GDP).

Results: There were a total of 4,830 cases reported in 100 (70.9%) counties and districts in Shandong Province from 2010 to 2020. The results showed that the annual average temperature, precipitation, forest and grassland coverage rate, altitude and meat production (square root transform) had a reversed "V" relationship with SFTS occurrence, with the inflection points around 12.5-13.0°C in temperature, around 650 mm in precipitation, around 0.3 in forest and grassland coverage rate, around 300 m in altitude, and around 200-300 tons in meat production (square root transform), respectively. SFTS occurrence had a "V" relationship with milk production (square root transform) and GDP (square root transform), with the inflection points around 100-200 tons in milk production (square root transform), and around 150,000-200,000 yuan in GDP (square root transform), respectively.

Conclusions: Climatic, environmental, and socioeconomic factors contributed to the heterogeneous distribution of SFTS in Shandong Province, and the influence of these factors on SFTS occurrence was nonlinear.
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http://dx.doi.org/10.3389/fpubh.2022.844220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8907623PMC
April 2022

Prognostic Value of Glasgow Prognostic Score in Non-small Cell Lung Cancer: A Systematic Review and Meta-Analysis.

Pathol Oncol Res 2022 15;28:1610109. Epub 2022 Feb 15.

International Medical Department of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Systemic inflammation is a key factor in tumor growth. The Glasgow Prognostic Score (GPS) has a certain value in predicting the prognosis of lung cancer. However, these results still do not have a unified direction. A systematic review and meta-analysis were performed to investigate the relationship between GPS and the prognosis of patients with non-small cell lung cancer (NSCLC). We set patients as follows: GPS = 0 vs. GPS = 1 or 2, GPS = 0 vs. GPS = 1, GPS = 0 vs. GPS = 2. We collected the hazard ratio (HR) and the 95% confidence interval (CI). A total of 21 studies were included, involving 7333 patients. We observed a significant correlation with GPS and poor OS in NSCLC patients (HR = 1.62, 95% CI: 1.27-2.07, ≤ .001; HR = 2.14, 95% CI:1.31-3.49, ≤ .001; HR = 2.64, 95% CI: 1.45-4.82, ≤ .001). Moreover, we made a subgroup analysis of surgery and stage. The results showed that when divided into GPS = 0 group and GPS = 1 or 2 group, the effect of high GPS on OS was more obvious in surgery (HR = 1.79, 95% CI: 1.08-2.97, = .024). When GPS was divided into two groups (GPS = 0 and GPS = 1 or 2), the III-IV stage, higher GPS is associated with poor OS (HR = 1.73, 95% CI: 1.43-2.09, ≤ .001). In the comparison of GPS = 0 and GPS = 1 group (HR = 1.56, 95% CI: 1.05-2.31, = .026) and the grouping of GPS = 0 and GPS = 2(HR = 2.23, 95% CI: 1.17-4.26, = .015), we came to the same conclusion. For patients with NSCLC, higher GPS is associated with poor prognosis, and GPS may be a reliable prognostic indicator. The decrease of GPS after pretreatment may be an effective way to improve the prognosis of NSCLC.
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http://dx.doi.org/10.3389/pore.2022.1610109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885527PMC
March 2022

Human motor learning is robust to control-dependent noise.

Biol Cybern 2022 Mar 3. Epub 2022 Mar 3.

Department of Electrical and Computer Engineering, New York University, 370 Jay Street, Brooklyn, NY, 11201, USA.

Noises are ubiquitous in sensorimotor interactions and contaminate the information provided to the central nervous system (CNS) for motor learning. An interesting question is how the CNS manages motor learning with imprecise information. Integrating ideas from reinforcement learning and adaptive optimal control, this paper develops a novel computational mechanism to explain the robustness of human motor learning to the imprecise information, caused by control-dependent noise that exists inherently in the sensorimotor systems. Starting from an initial admissible control policy, in each learning trial the mechanism collects and uses the noisy sensory data (caused by the control-dependent noise) to form an imprecise evaluation of the performance of the current policy and then constructs an updated policy based on the imprecise evaluation. As the number of learning trials increases, the generated policies mathematically provably converge to a (potentially small) neighborhood of the optimal policy under mild conditions, despite the imprecise information in the learning process. The mechanism directly synthesizes the policies from the sensory data, without identifying an internal forward model. Our preliminary computational results on two classic arm reaching tasks are in line with experimental observations reported in the literature. The model-free control principle proposed in the paper sheds more lights into the inherent robustness of human sensorimotor systems to the imprecise information, especially control-dependent noise, in the CNS.
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http://dx.doi.org/10.1007/s00422-022-00922-zDOI Listing
March 2022

Revealing the contribution of somatic gene mutations to shaping tumor immune microenvironment.

Brief Bioinform 2022 May;23(3)

College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang 150081, China.

Interaction between tumor cells and immune cells determined highly heterogeneous microenvironments across patients, leading to substantial variation in clinical benefits from immunotherapy. Somatic gene mutations were found not only to elicit adaptive immunity but also to influence the composition of tumor immune microenvironment and various processes of antitumor immunity. However, due to an incomplete view of associations between gene mutations and immunophenotypes, how tumor cells shape the immune microenvironment and further determine the clinical benefit of immunotherapy is still unclear. To address this, we proposed a computational approach, inference of mutation effect on immunophenotype by integrated gene set enrichment analysis (MEIGSEA), for tracing back the genomic factor responsible for differences in immunophenotypes. MEIGSEA was demonstrated to accurately identify the previous confirmed immune-associated gene mutations, and systematic evaluation in simulation data further supported its performance. We used MEIGSEA to investigate the influence of driver gene mutations on the infiltration of 22 immune cell types across 19 cancers from The Cancer Genome Atlas. The top associated gene mutations with infiltration of CD8 T cells, such as CASP8, KRAS and EGFR, also showed extensive impact on other immune components; meanwhile, immune effector cells shared critical gene mutations that collaboratively contribute to shaping distinct tumor immune microenvironment. Furthermore, we highlighted the predictive capacity of gene mutations that are positively associated with CD8 T cells for the clinical benefit of immunotherapy. Taken together, we present a computational framework to help illustrate the potential of somatic gene mutations in shaping the tumor immune microenvironment.
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http://dx.doi.org/10.1093/bib/bbac064DOI Listing
May 2022

A Cohort Study to Assess Cognitive Impairment and Its Effects on Older Patients in the Orthopedic Rehabilitation.

J Healthc Eng 2022 18;2022:7821525. Epub 2022 Feb 18.

Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: The cognitive role of older patients is regularly not investigated in orthopedic rehabilitation, after the elective as well as the nonelective operation. The objective of this research is to investigate the cognitive disorder and its influence over the duration of stay along with the functional consequences of the older patients who were admitted to orthopedic rehabilitation. . The inclusion criteria for this study were the patients with age above 50 years; who were admitted with the detection of orthopedic impairment and the surgery both elective and nonelective, investigated utilizing the MoCA (Montreal Cognitive Assessment) over admission, MBI (Modified Barthel Index), and FIM (Function Independent Measure) over admission and discharge status; and were discharged from the hospital. The demography, as well as the clinical data, comprising of the duration of stay, age and the detection was also reported.

Result: Of the 109 admitted patients, 80 patients were included in the study where  = 47 (58.75%) patients were females and  = 33 (41.25%) were males. The age group range was 50 to 94 years with a mean age of 78.5 years (SD = 8.27). The diagnostic groups included for the study were fractured neck of femur ( = 34; 42.5%), orthopedic surgery ( = 22; 27.5%), and other orthopedic surgery ( = 24; 30%). The mean duration of rehabilitation stay was reported as 34 (4.39), where the MoCA was reported as 22.17 (2.44); functional independence measures were as follows: motor admission as 53.97 (7.55), motor discharge as 76.27 (5.35), cognitive admission as 30.71 (1.99), and cognitive discharge as 31.85 (1.94). Here, the diagnosis was done over the fractured neck of the femur (i.e., NOF being 34 (42.5%), elective surgeries 22 (27.5%), and other orthopedic as 24 (30%)).

Conclusion: An excessive percentage of older-age patients in a rehabilitation unit with elective as well as nonelective diagnoses comprises the cognitive disorder. The cognitive screening was advised for all the older age patients in the rehabilitation units to report a specific rehabilitation plan to enhance the consequences along with the duration of stay. There is further study required to explore different cognitive strategies to enhance the rehabilitation consequences among older-age orthopedic patients.
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http://dx.doi.org/10.1155/2022/7821525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881174PMC
May 2022

IQSEC3 Deletion Impairs Fear Memory Through Upregulation of Ribosomal S6K1 Signaling in the Hippocampus.

Biol Psychiatry 2022 05 31;91(9):821-831. Epub 2021 Dec 31.

Department of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea. Electronic address:

Background: IQSEC3, a gephyrin-binding GABAergic (gamma-aminobutyric acidergic) synapse-specific guanine nucleotide exchange factor, was recently reported to regulate activity-dependent GABAergic synapse maturation, but the underlying signaling mechanisms remain incompletely understood.

Methods: We generated mice with conditional knockout (cKO) of Iqsec3 to examine whether altered synaptic inhibition influences hippocampus-dependent fear memory formation. In addition, electrophysiological recordings, immunohistochemistry, and behavioral assays were used to address our question.

Results: We found that Iqsec3-cKO induces a specific reduction in GABAergic synapse density, GABAergic synaptic transmission, and maintenance of long-term potentiation in the hippocampal CA1 region. In addition, Iqsec3-cKO mice exhibited impaired fear memory formation. Strikingly, Iqsec3-cKO caused abnormally enhanced activation of ribosomal P70-S6K1-mediated signaling in the hippocampus but not in the cortex. Furthermore, inhibiting upregulated S6K1 signaling by expressing dominant-negative S6K1 in the hippocampal CA1 of Iqsec3-cKO mice completely rescued impaired fear learning and inhibitory synapse density but not deficits in long-term potentiation maintenance. Finally, upregulated S6K1 signaling was rescued by IQSEC3 wild-type, but not by an ARF-GEF (adenosine diphosphate ribosylation factor-guanine nucleotide exchange factor) inactive IQSEC3 mutant.

Conclusions: Our results suggest that IQSEC3-mediated balanced synaptic inhibition in hippocampal CA1 is critical for the proper formation of hippocampus-dependent fear memory.
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http://dx.doi.org/10.1016/j.biopsych.2021.12.016DOI Listing
May 2022

Transcriptome Analysis of Peripheral Blood Mononuclear Cells in SARS-CoV-2 Naïve and Recovered Individuals Vaccinated With Inactivated Vaccine.

Front Cell Infect Microbiol 2021 3;11:821828. Epub 2022 Feb 3.

Ministry of Research and Education, Shandong Center for Disease Control and Prevention, Jinan, China.

The urgent approval of the use of the inactivated COVID-19 vaccine is essential to reduce the threat and burden of the epidemic on global public health, however, our current understanding of the host immune response to inactivated vaccine remains limited. Herein, we performed serum IgG antibody detection and transcriptomics analysis on 20 SARS-CoV-2 naïve individuals who received multiple doses of inactivated vaccine and 5 SARS-CoV-2 recovered individuals who received single dose of inactivated vaccine. Our research revealed the important role of many innate immune pathways after vaccination, identified a significant correlation with the third dose of booster vaccine and proteasome-related genes, and found that SARS-CoV-2 recovered individuals can produces a strong immune response to a single dose of inactivated vaccine. These results help us understand the reaction mechanism of the host's molecular immune system to the inactivated vaccine, and provide a basis for the choice of vaccination strategy.
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http://dx.doi.org/10.3389/fcimb.2021.821828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851474PMC
February 2022

Strategy for Avoiding Protein Corona Inhibition of Targeted Drug Delivery by Linking Recombinant Affibody Scaffold to Magnetosomes.

Int J Nanomedicine 2022 11;17:665-680. Epub 2022 Feb 11.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, People's Republic of China.

Purpose: Nanoparticles (NPs) decorated with functional ligands are promising candidates for cancer diagnosis and treatment. However, numerous studies have shown that chemically coupled targeting moieties on NPs lose their targeting capability in the biological milieu because they are shielded or covered by a "protein corona". Herein, we construct a functional magnetosome that recognizes and targets cancer cells even in the presence of protein corona.

Methods: Magnetosomes (BMPs) were extracted from magnetotactic bacteria, (MSR-1), and decorated with trastuzumab (TZ) via affibody (RA) and glutaraldehyde (GA). The engineered BMPs are referred to as BMP-RA-TZ and BMP-GA-TZ. Their capacities to combine HER2 were detected by ELISA, the quantity of plasma corona proteins was analyzed using LC-MS. The efficiencies of targeting SK-BR-3 were demonstrated by confocal laser scanning microscopy and flow cytometry.

Results: Both engineered BMPs contain up to ~0.2 mg TZ per mg of BMP, while the quantity of HER2 binding to BMP-RA-TZ is three times higher than that binding to BMP-GA-TZ. After incubation with normal human plasma or IgG-supplemented plasma, GA-TZ-containing BMPs have larger hydrated radii and more surface proteins in comparison with RA-TZ-containing BMPs. The TZ-containing BMPs all can be targeted to and internalized in the HER2-overexpressing breast cancer cell line SK-BR-3; however, their targeting efficiencies vary considerably: 50-75% for RA-TZ-containing BMPs and 9-19% for GA-TZ-containing BMPs. BMPs were incubated with plasma (100%) and cancer cells to simulate human in vivo environment. In this milieu, BMP-RA-TZ uptake efficiency of SK-BR-3 reaches nearly 80% (slightly lower than for direct interaction with BMP-RA-TZ), whereas the BMP-GA-TZ uptake efficiency is <17%.

Conclusion: Application of the RA scaffold promotes and orients the arrangement of targeting ligands and reduces the shielding effect of corona proteins. This strategy improves the targeting capability and drug delivery of NP in a simulated in vivo milieu.
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http://dx.doi.org/10.2147/IJN.S338349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847798PMC
February 2022

Regulation of the Interfaces Between Argyrodite Solid Electrolytes and Lithium Metal Anode.

Front Chem 2022 1;10:837978. Epub 2022 Feb 1.

College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou, China.

Lithium-ion batteries (LIBs) are widely used in portable electronic devices, electric vehicles and large scale energy storage, due to their considerable energy density, low cost and long cycle life. However, traditional liquid batteries suffer from safety problems such as leakage, thermal runaway and even explosion. Part of the issues are caused by lithium dendrites puncturing the liquid electrolyte during cycling. In order to achieve the objective of higher safety and energy density, a rigid solid-state electrolyte (SSE) is proposed instead of liquid electrolyte (LE). Thereinto, sulfide SSEs have received of the most attention due to their high ionic conductivity. Among all the sulfide SSEs, argyrodite SSEs are considered to be one of the most promising solid-state electrolytes due to their high ionic conductivity, high thermal stability and good processablity. On the other hand, lithium metal is an ideal material for anode because of its high specific energy, low potential and large storage capacity. However, interfacial problems between argyrodite SSEs and the anode (interfacial reactions, lithium dendrites, etc.) are considered to be important factors affecting their availability. In this mini review, we summarize the behavior, properties and problems arising at the interface between argyrodite SSEs and anode. Strategies to solve interface problems and stabilize interfaces in recent years are also discussed. Finally, a brief outlook about argyrodite SSEs is presented.
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http://dx.doi.org/10.3389/fchem.2022.837978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844468PMC
February 2022

Ambient particulate air pollution, blood cell parameters, and effect modification by psychosocial stress: Findings from two studies in three major Chinese cities.

Environ Res 2022 Jul 14;210:112932. Epub 2022 Feb 14.

Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China; Key Laboratory for Disease Prevention and Control and Health Promotion of Shaanxi Province, Xi'an, Shaanxi, China; Key Laboratory of Trace Elements and Endemic Diseases in Ministry of Health, Xi'an, Shaanxi, China. Electronic address:

The associations between particulate matter (PM) exposure, psychosocial stress and blood cell parameters are bringing novel insights to characterize the early damage of multiple diseases. Based on two studies conducted in three Chinses cities using cross-sectional (Beijing, 425 participants) and panel study (Tianjin and Shanghai, 92 participants with 361 repeated measurements) designs, this study explored the associations between short-term exposure to ambient PM and blood cell parameters, and the effect modification by psychosocial stress. Increasing PM exposure was significantly associated with decreases in red blood cell (RBC) count and mean corpuscular hemoglobin concentration (MCHC), and increases in mean corpuscular volume (MCV), platelets count (PLT) and platelet hematocrit (PCT) in both studies. For instance, a 10 μg/m increment in PM concentration was associated with a 1.04% (95%CI: 0.16%, 1.92%) increase in PLT (4-d) and a 1.09% (95%CI: 0.31%, 1.87%) increase in PCT (4-d) in the cross-sectional study, and a 0.64% (95%CI: 0.06%, 1.22%) increase in PLT (1-d) and a 0.72% (95%CI: 0.33%, 1.11%) increase in PCT (1-d) in the panel study, respectively. In addition, stronger increases in MCV, PLT, and PCT associated with PM exposure were found in higher psychosocial stress group compared to lower psychosocial stress group (p for interaction <0.10), indicating that blood cell parameters of individuals with higher psychosocial stress might be more susceptible to the early damages of PM exposure.
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http://dx.doi.org/10.1016/j.envres.2022.112932DOI Listing
July 2022

Dioscin promotes autophagy by regulating the AMPK-mTOR pathway in ulcerative colitis.

Immunopharmacol Immunotoxicol 2022 Apr 17;44(2):238-246. Epub 2022 Feb 17.

Department of Geriatrics, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, China.

Background: Dioscin is reported to alleviate the dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice. Autophagy plays an anti-inflammatory role in UC. We herein aimed to explore the biological functions of dioscin in autophagy in UC.

Methods: To explore the effects of dioscin on UC progression, a DSS-induced mouse model of UC was established. Body weight, disease activity index and macroscopic damage index scores were recorded for seven days. Hematoxylin & Eosin (HE) staining was used to stain colon sections and an BX53 microscope was prepared to observe pathological changes. The activities of glutathione, superoxidative dismutase, and malondialdehyde were determined by commercially available kits. Western blotting was performed to measure the protein levels of p-AMPK/AMPK, p-mTOR/mTOR and autophagy-related genes.

Results: The DSS-induced colitis and oxidative stress in mice were ameliorated after dioscin treatment. Dioscin promoted the phosphorylation of AMPK to inhibit mTOR activation and facilitated autophagy in DSS-induced mice model of UC.

Conclusion: Dioscin promotes autophagy by promoting the phosphorylation of AMPK to inhibit mTOR activation in ulcerative colitis.
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http://dx.doi.org/10.1080/08923973.2022.2037632DOI Listing
April 2022

The modifying effect of trait anxiety on the association of fine particulate matter with heart rate variability variables.

Int J Hyg Environ Health 2022 04 5;241:113933. Epub 2022 Feb 5.

Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China; Key Laboratory of Trace Elements and Endemic Diseases in Ministry of Health, Xi'an, Shaanxi, China; Key Laboratory for Disease Prevention and Control and Health Promotion of Shaanxi Province, Xi'an, Shaanxi, China. Electronic address:

Background: Previous studies have shown that exposures to ambient particulate matter with aerodynamic diameter <2.5 μm (PM) and stress are associated with adverse cardiovascular health effects.

Objectives: To investigate the potential modifying effect of trait anxiety on the association between short-term exposures to PM and HRV variables.

Methods: A panel of 92 middle-aged and elderly adults in Tianjin and Shanghai were recruited for repeated follow-ups with measurements of 24-h personal exposures to air pollutants and Holter ECG monitoring. Heart rate variability (HRV) variables calculated over 5-minute segments during the 24 h, including low frequency power (LF), high frequency power (HF), standard deviation of normal-to-normal intervals (SDNN) and root mean square of successive differences (rMSSD), were included in the analysis. The Trait Anxiety Inventory was used to investigate the long-term general anxiety level of the participants. Generalized linear mixed-effects model was used to analyze the association between exposure factors and HRV variables, and potential effect modification by trait anxiety.

Results: Data on 87 participants were included in final analysis after exclusions. Higher exposure to PM was associated with lower levels of LF, HF, SDNN and rMSSD, and the largest decreases in LF, HF, SDNN and rMSSD were found at 3-h moving average. Trait anxiety significantly modified the associations of PM with LF, HF, SDNN and rMSSD, with stronger inverse associations found in high trait anxiety group than in low trait anxiety group. For an IQR (27.3 μg/m³) increase in PM at 3-h moving average, there were decreases of 3.50% (95% CI: -4.46%, -2.54%) and 3.50% (95% CI: -4.49%, -2.50%) in the high trait anxiety group, and decreases of 0.81% (95% CI: -1.22%, -0.40%) and 0.65% (95% CI: -1.07%, -0.23%) in the low trait anxiety group in HF and rMSSD, respectively (both p for interaction<0.01).

Conclusion: Our study suggests that trait anxiety could modify the association of short-term exposure to PM with HRV variables, indicating that higher trait anxiety may increase the cardiac susceptibility to air pollution in the study participants.
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http://dx.doi.org/10.1016/j.ijheh.2022.113933DOI Listing
April 2022

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an mA-dependent manner.

Cell Death Dis 2022 01 21;13(1):73. Epub 2022 Jan 21.

Department of Neurosurgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Ferroptosis is a form of cell death characterized by lipid peroxidation. Previous studies have reported that knockout of NF-κB activating protein (NKAP), an RNA-binding protein, increased lipid peroxidation level in naive T cells and induced cell death in colon cancer cells. However, there was no literature reported the relationship between NKAP and ferroptosis in glioblastoma cells. Notably, the mechanism of NKAP modulating ferroptosis is still unknown. Here, we found NKAP knockdown induced cell death in glioblastoma cells. Silencing NKAP increased the cell sensitivity to ferroptosis inducers both in vitro and in vivo. Exogenous overexpression of NKAP promoted cell resistance to ferroptosis inducers by positively regulating a ferroptosis defense protein, namely cystine/glutamate antiporter (SLC7A11). The regulation of SLC7A11 by NKAP can be weakened by the mA methylation inhibitor cycloleucine and knockdown of the mA writer METTL3. NKAP combined the "RGAC" motif which was exactly in line with the mA motif "RGACH" (R = A/G, H = A/U/C) uncovered by the mA-sequence. RNA Immunoprecipitation (RIP) and Co-Immunoprecipitation (Co-IP) proved the interaction between NKAP and mA on SLC7A11 transcript. Following its binding to mA, NKAP recruited the splicing factor proline and glutamine-rich (SFPQ) to recognize the splice site and then conducted transcription termination site (TTS) splicing event on SLC7A11 transcript and the retention of the last exon, screened by RNA-sequence and Mass Spectrometry (MS). In conclusion, NKAP acted as a new ferroptosis suppressor by binding to mA and then promoting SLC7A11 mRNA splicing and maturation.
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http://dx.doi.org/10.1038/s41419-022-04524-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783023PMC
January 2022

Investigation of 2,2'-Bipyridine Biosynthesis Reveals a Common Two-Component System for Aldehydes Production by Carboxylate Reduction.

Org Lett 2022 01 19;24(3):897-902. Epub 2022 Jan 19.

State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.

Here, we report a two-component enzymatic system that efficiently catalyzes the reduction of a carboxylate to an aldehyde in the biosynthesis of 2,2'-bipyridine antibiotics caerulomycins. The associated paradigm involves the activation of carboxylate by ATP-dependent adenylation protein CaeF, followed by its reduction catalyzed by CaeB2, a new class of NADPH-dependent aldehyde dehydrogenase (ALDH) that directly reduces AMP-conjugated carboxylate, which is distinct from the known aldehyde-producing enzymes that reduce ACP- or CoA-conjugated carboxylates.
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http://dx.doi.org/10.1021/acs.orglett.1c04239DOI Listing
January 2022
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