Publications by authors named "Bo Liu"

3,643 Publications

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Labeling of liver cells with CdSe/ZnS quantum dot-based fluorescence probe below freezing point.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 21;263:120203. Epub 2021 Jul 21.

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China; Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China. Electronic address:

In this paper, CdSe/ZnS quantum dots (QDs) with particle size of 5.5 ~ 9.3 nm were synthesized, and the fluorescence emission ranged from 545 ~ 616 nm. When the volume fraction of ethanol was 30%, the water-soluble QD dispersion system remained liquid under -20 °C freezing conditions, the fluorescence intensity increased with a decrease in temperature, and the quantum yield reached 79% at -20 °C. The endothelial cell adhesion molecule CD31 antibody (anti-CD31) was used as the primary antibody, QDs were coupled with IgG as the secondary antibody (QD-Ab), and effective labeling of hepatic sinusoid endothelial cells was achieved at -20 °C. Fluorescence imaging and flow cytometry analysis showed that the labeling efficiency was as high as 97%, indicating that QDs have an important application prospect in microscopic section tomography of the liver.
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http://dx.doi.org/10.1016/j.saa.2021.120203DOI Listing
July 2021

Disrupted Topological Organization in White Matter Networks in Unilateral Sudden Sensorineural Hearing Loss.

Front Neurosci 2021 12;15:666651. Epub 2021 Jul 12.

Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Sudden sensorineural hearing loss (SSNHL) is a sudden-onset hearing impairment that rapidly develops within 72 h and is mostly unilateral. Only a few patients can be identified with a defined cause by routine clinical examinations. Recently, some studies have shown that unilateral SSNHL is associated with alterations in the central nervous system. However, little is known about the topological organization of white matter (WM) networks in unilateral SSNHL patients in the acute phase. In this study, 145 patients with SSNHL and 91 age-, gender-, and education-matched healthy controls were evaluated using diffusion tensor imaging (DTI) and graph theoretical approaches. The topological properties of WM networks, including global and nodal parameters, were investigated. At the global level, SSNHL patients displayed decreased clustering coefficient, local efficiency, global efficiency, normalized clustering coefficient, normalized characteristic path length, and small-worldness and increased characteristic path length ( < 0.05) compared with healthy controls. At the nodal level, altered nodal centralities in brain regions involved the auditory network, visual network, attention network, default mode network (DMN), sensorimotor network, and subcortical network ( < 0.05, Bonferroni corrected). These findings indicate a shift of the WM network topology in SSNHL patients toward randomization, which is characterized by decreased global network integration and segregation and is reflected by decreased global connectivity and altered nodal centralities. This study could help us understand the potential pathophysiology of unilateral SSNHL.
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http://dx.doi.org/10.3389/fnins.2021.666651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312563PMC
July 2021

Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway.

J Mol Histol 2021 Jul 23. Epub 2021 Jul 23.

Center of Osteoporosis and Bone Mineral Research, Shandong University, Jinan, China.

The apoptosis of mature osteocytes is the main factor causing damage to the microstructure of cortical bone in glucocorticoid-induced osteoporosis (GIOP). Our previous research found damaged areas and empty osteocytes lacunae in the tibial cortical bone of GIOP mice. However, the specific mechanism has not been clarified. Recently, a study showed that the quality of the cortical bone significantly increased by knocking out Notum, a gene encoding α/β hydrolase. However, it is not clear whether Notum affects cortical bone remodeling by participating in glucocorticoids (GCs)-induced apoptosis of osteocytes. The present study aimed to explore the correlation between Notum, osteocytes apoptosis, and cortical bone quality in GIOP. Prednisolone acetate was intragastrically administered to mice for two weeks. Histochemical staining was applied to evaluate changes in GIOP and Notum expression. Osteocytes were stimulated with prednisolone, and cell viability was assessed via CCK8. Hoechst 33342/PI staining, flow cytometry, RT-PCR, and western blot were used to detect osteocytes apoptosis, siRNA transfection efficiency, and expressions of pathway related factors. The results showed that the number of empty osteocytes lacunae increased in GIOP mice. TUNEL-stained apoptotic osteocytes and Notum immuno-positive osteocytes were also observed. Furthermore, prednisolone was found to promote Notum expression and osteocytes apoptosis in vitro. Knocking down Notum via siRNA partially restored osteocytes apoptosis and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/glycogen synthase kinase-3β (GSK3β)/β-catenin pathway. These findings showed GCs-induced osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway. Thus, Notum might be a potential therapeutic target for the treatment of GIOP.
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http://dx.doi.org/10.1007/s10735-021-10006-0DOI Listing
July 2021

Long non-coding RNA H19 acts as a microRNA-194 sponge to inhibit the apoptosis and promote the proliferation of hypertrophic scar fibroblasts.

Can J Physiol Pharmacol 2021 Jul 26. Epub 2021 Jul 26.

Medical School of Eastern Liaoning University, Department of Cosmetic Dermatology, Dandong, China;

The effects of long non-coding RNAs (lncRNAs) on the proliferation of hypertrophic scars have been described. However, the underlying mechanisms are not well characterized. The present study aimed to investigate the mechanisms of lncRNA H19 in hypertrophic scars. The effects of the lncRNA H19 on the proliferation and apoptosis of hypertrophic scar fibroblasts (HSFs) were analyzed using 5'-Ethynyl-2'-deoxyuridine staining, flow cytometry, and MTT. The results revealed H19 promoted the proliferation and inhibited the apoptosis in HSF. In addition, the binding associations between H19 and microRNA-194 (miR-194), and miR-194 and insulin-like growth factor-I receptor (IGF1R) were identified using bioinformatics screening and verified using dual-luciferase assays. Furthermore, the effects of the IGF1R knockdown on H19-induced HSF phenotypes and regulation over the p38 MAPK pathway were determined. Mechanistically, miR-194 was identified as the downstream effector of the H19-mediated phenotypes of HSFs through its ability to directly target IGF1R, thus modulating the p38 MAPK signaling pathway. In conclusion, the findings suggested that H19 may inhibit the apoptosis and promote the proliferation of HSFs through the miR-194/IGF1R/p38 MAPK signaling axis, thereby contributing to the progression of hypertrophic scars. These findings may provide novel targets for the treatment of hypertrophic scars.
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http://dx.doi.org/10.1139/cjpp-2021-0351DOI Listing
July 2021

The Combination of Electroacupuncture and Massage Therapy Alleviates Myofibroblast Transdifferentiation and Extracellular Matrix Production in Blunt Trauma-Induced Skeletal Muscle Fibrosis.

Evid Based Complement Alternat Med 2021 7;2021:5543468. Epub 2021 Jul 7.

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China.

Complementary therapies, such as acupuncture and massage, had been previously reported to have therapeutic effects on skeletal muscle contusions. However, the recovery mechanisms on skeletal muscles after blunt trauma via the combination of electroacupuncture (EA) and massage therapy remain unclear. In the present study, a rat model of the skeletal muscle fibrosis following blunt trauma to rat skeletal muscle was established, and the potential molecular mechanisms of EA + massage therapy on the skeletal muscle fibrosis were investigated. The results suggested that EA + massage therapy could significantly decrease inflammatory cells infiltration and collagenous fiber content and ameliorate the disarrangement of sarcomeres within myofibrils compared to the model group. Further analysis revealed that EA + massage therapy could reduce the degree of fibrosis and increase the degree of myofibroblast apoptosis by downregulating the mRNA and protein expression of transforming growth factor- (TGF-) 1 and connective tissue growth factor (CTGF). Furthermore, the fibrosis of injured skeletal muscle was inhibited after treatment through the normalization of balance between matrix metalloproteinase- (MMP-) 1 and tissue inhibitor of matrix metalloproteinase (TIMP). These findings suggested that the combination of electroacupuncture and massage therapy could alleviate the fibrotic process by regulating TGF 1-CTGF-induced myofibroblast transdifferentiation and MMP-1/TIMP-1 balance for extracellular matrix production.
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http://dx.doi.org/10.1155/2021/5543468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282377PMC
July 2021

Comparative Genomics Provide Insights Into Function and Evolution of Odorant Binding Proteins in .

Front Physiol 2021 7;12:690185. Epub 2021 Jul 7.

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Insect olfaction is vital for foraging, mating, host-seeking, and avoidance of predators/pathogens. In insects, odorant binding proteins (OBPs) are involved in transporting hydrophobic odor molecules from the external environment to receptor neurons. The codling moth, , one of the most destructive insect fruit pests, causes enormous economic losses. However, little is known about the number, variety, gains and losses, and evolution of genes in . Here we report the identification of 40 in , most (75%) of which are classic , using genomic and transcriptomic analyses. Two genes were lost in relative to possible distant ancestor in Lepidoptera lineage based on an analysis of gene gains and losses. The phylogenetic tree and chromosome location showed that the expansion of genes mainly resulted from tandem duplications, as the gene was duplicated twice along with loss of . Two positive selection sites of the gene were identified while other genes evolved under purifying selection. Our results provide fundamental knowledge of genes allowing further study of their function in .
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http://dx.doi.org/10.3389/fphys.2021.690185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294088PMC
July 2021

[The relationship between vestibular function and gait parameters in vestibular dysfunctional patients with idiopathic sudden sensorineural hearing loss].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Jul;35(7):636-640

Department of Otorhinolaryngology Head and Neck Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China.

Based on the application of vHIT, cVEMP test and oVEMP test to locate the peripheral vestibular lesions, we aimed to explore the relationship between vestibular function(semicircular canal and otolith function) and gait parameters in vestibular dysfunctional patients with idiopathic sudden sensorineural hearing loss(ISSNHL). In this study, 23 ISSNHL patients(including 14 patients with vertigo) were enrolled. All patients underwent audiometry, Walk Across, vHIT, cVEMP test and oVEMP test. Vestibular otolith function was significantly associated with gait parameters. Semicircular canal function was not associated with any gait parameter. Reduced otolith function was associated with slower and wider steps in patients with ISSNHL. These results indicate that vestibular signals may contribute to specific aspects of gait.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.07.013DOI Listing
July 2021

[Sensory organization test results for maintaining an upright balance in patients with type 2 diabetes mellitus and patients with peripheral vestibular disorders].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Jul;35(7):581-585

Department of Endocrinology,Beijing Anzhen Hospital,Capital Medical University.

To research the differences of sensory organization testing in maintaining postural stability between patients with type 2 diabetes mellitus and patients with peripheral vertigo using computerized posturography. Participants were divided into the control group (52 cases), the type 2 diabetes mellitus group (T2DM) (45 cases), and the peripheral vertigo group (PV) (47 cases). All participants were examined under six conditions by computerized posturography: The sensory organization test, a part of computerized dynamic posturography, was used to assess the abilities of vision, somatosensory and vestibular systems in maintaining postural stability. The scores of statokinesiogram (SKG) of the T2DM group in condition 1 (standing on static platform with eye open), condition 4(standing on foam platform with eyes open) and condition 6(standing on foam platform with servo-controlled vision) were significantly greater than that in the vertigo group (<0.01). The visual scores in the T2DM group were lower than those of the PV group(<0.01) in the anteroposterior and lateral directions. Patients with type 2 diabetes and peripheral vertigo have a decreased ability to maintain balance in the upright position. Patients with type 2 diabetes have a poorer ability to maintain balance with visual systems than patients with peripheral vertigo.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.07.002DOI Listing
July 2021

Persistent fifth aortic arch: a single-center experience, case series.

Transl Pediatr 2021 Jun;10(6):1566-1572

Division of Cardiology, the Affiliated Children's Hospital of Capital Institute of Pediatrics, Beijing, China.

Background: Persistent fifth aortic arch (PFAA) is an extremely rare congenital cardiovascular malformation and there is limited data in the literature. The objective of this study is to enhance our understanding and diagnosis of PFAA from echocardiography and computed tomography angiography (CTA) findings, and to evaluate the application of echocardiography in the diagnosis of PFAA.

Methods: We retrospectively reviewed five cases of PFAA diagnosed from October 2016 to September 2019 at the Affiliated Children's Hospital of Capital Institute of Pediatrics. We described their diagnosis by echocardiography and CTA findings, and medical history.

Results: Five cases of PFAA were identified in the study. Patients aged from 3 to 48 months and weighed from 4 to 12 kg presented different clinical symptoms upon clinical examination. All the patients completed a primary echocardiographic assessment; however, the first two patients were misdiagnosed by echocardiography and was confirmed by supplemental CTA while the other three patients were directly diagnosed by echocardiography. Surgery was necessary for three patients, two of whom accepted and one refused. The other two patients only needed a follow-up assessment, which showed good results.

Conclusions: The clinical manifestation of PFAA in our patient population was atypical, and their diagnosis depended on the use of echocardiography. In the case of uncertainty, the final diagnosis was confirmed by CTA. Although the nomenclature and embryonic origin of PFAA remains controversial, the accurate diagnosis of aortic arch abnormalities and associated malformations are imperative for time-sensitive treatments.
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http://dx.doi.org/10.21037/tp-20-433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261588PMC
June 2021

[email protected] Nanoparticles for Photodynamic/Photothermal Combined Cancer Therapy with Single Near-Infrared Irradiation.

Int J Nanomedicine 2021 16;16:4863-4871. Epub 2021 Jul 16.

Laboratory of Veterinary Pathology and Nanopathology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.

Background: Phototherapy has significant potential as an effective treatment for cancer. However, the application of a multifunctional nanoplatform for photodynamic therapy (PDT) and photothermal therapy (PTT) at a single excitation wavelength remains a challenge.

Materials And Methods: The double emulsion solvent evaporation method was used to prepare toluidine [email protected] lactic-co-glycolic acid ([email protected]) nanoparticles (NPs). The biocompatibility of [email protected] NPs was evaluated, and a 660 nm luminescence was used as the light source. The photothermal effect, photothermal stability, and singlet oxygen yield of NPs in an aqueous solution verified the feasibility of NPs as a PTT/PDT synergistic therapy drug.

Results: [email protected] NPs were successfully prepared and characterized. In vitro experiments demonstrated that [email protected] NPs can cause massive necrosis of tumor cells and induce apoptosis through a photodynamic mechanism under 660 nm laser irradiation. The [email protected] NPs also achieved optimal tumor inhibition effect in vivo.

Conclusion: The [email protected] NPs prepared in this study were applied as a dual-mode phototherapeutic agent under single laser irradiation. Both in vitro and in vivo experiments demonstrated the good potential of PTT/PDT for tumor inhibitors.
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http://dx.doi.org/10.2147/IJN.S304713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291662PMC
July 2021

Blockade of GITRL/GITR signaling pathway attenuates house dust mite-induced allergic asthma in mice through inhibition of MAPKs and NF-κB signaling.

Mol Immunol 2021 Jul 20;137:238-246. Epub 2021 Jul 20.

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, PR China; National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, PR China. Electronic address:

GITRL/GITR signaling pathway plays an important role in allergy, inflammation, transplantation and autoimmunity. However, its role in asthma remains unclear. Thus, the present study aimed to investigate changes in this pathway and observe the therapeutic effect of its blocking on asthma. By using house dust mite-induced asthma model, changes of GITRL/GITR and its downstream molecules MAPKs (e.g., p38 MAPK, JNK and Erk) and NF-κB were observed. After that, GITRL in lung of mice was knocked down by recombinant adeno-associated virus to observe the impact on its downstream molecules and assess the therapeutic effect on asthma. These results showed that GITRL/GITR and its downstream molecules MAPKs/NF-κB were activated in asthmatic mice. This activation was suppressed after GITRL knockdown, and allergic airway inflammation and airway hyperresponsiveness were alleviated. These results demonstrate that GITRL/GITR-MAPKs/NF-κB signaling pathway participates in the pathogenesis of asthma. Blockade of GITRL/GITR signaling pathway exhibits protective effects in a mouse model of house dust mite-induced allergic asthma.
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http://dx.doi.org/10.1016/j.molimm.2021.07.005DOI Listing
July 2021

Do perfluoroalkyl substances aggravate the occurrence of obesity-associated glucolipid metabolic disease?

Environ Res 2021 Jul 19;202:111724. Epub 2021 Jul 19.

College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, China.

Background: Since 2016, more and more studies have been conducted to explore the combination of obesity and perfluoroalkyl substances (PFASs) exposure, and the results indicate that PFASs may be connected with the occurrence of obesity-associated glucolipid metabolic disease (GLMD).

Objectives: This article summarizes the epidemiological studies on PFASs and obesity-related GLMD, as well as relevant experimental evidence.

Results: (i) Both obesity and PFASs exposure can cause disorder of glucose and lipid metabolism (GLM). (ii) Obesity is a pivotal factor in the high incidence of GLMD induce by PFASs. (iii) PFASs are aggravating the occurrence of obesity-associated GLMD [e.g., diabetes, cardiovascular disease (CVD), and liver disease].

Conclusion: The paper fills the gaps among environmental chemistry/epidemiology/toxicology area research. More importantly, PFASs should be taken into account to explain the high-prevalence of obesity-related GLMD.

Future Direction: Three research programs are proposed to explore the synergistic mechanism of PFASs and obesity. In addition, three suggestions are recommended to solve the harm of PFASs pollutants to human beings.
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http://dx.doi.org/10.1016/j.envres.2021.111724DOI Listing
July 2021

Retainable Superconductivity and Structural Transition in 1T-TaSe Under High Pressure.

Inorg Chem 2021 Jul 21. Epub 2021 Jul 21.

State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, People's Republic of China.

As a prominent platform possessing the properties of superconductivity (SC) and charge density wave (CDW), transition-metal dichalcogenides (TMDCs) have attracted considerable attention for a long time. Moreover, extensive efforts have been devoted for exploring the SC and/or the interplay between SC and CDW in TMDCs in the past few decades. Here, we systematically investigate the electronic properties and structural evolution of 1T-TaSe under pressure. With increasing pressure, pressure-induced superconductivity is observed at ∼2.6 GPa. The superconductive transition temperature () increases with the suppression of the CDW state to the maximum value of ∼5.1 K at 21.8 GPa and then decreases monotonously up to the highest pressure of 57.8 GPa. 1T-TaSe transforms into a monoclinic 2/ structure above 19 GPa. The monoclinic phase coexists with the original phase as the pressure is released under ambient conditions and the retainable superconductivity with = 2.9 K is observed in the released sample. We suggest that the retained superconductivity can be ascribed to the retention of the superconductive high-pressure monoclinic phase in the released sample. Our findings demonstrate that both the structure and CDW order are related to the superconductivity of TaSe.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01378DOI Listing
July 2021

MicroRNA miR-215-5p regulates doxorubicin-induced cardiomyocyte injury by targeting ZEB2.

J Cardiovasc Pharmacol 2021 Jul 16. Epub 2021 Jul 16.

Department of Cardiovascular Medicine, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, Hubei, China.

Abstract: Doxorubicin (DOX) is a chemotherapeutic drug for treating various cancers. However, the DOX-induced cardiotoxicity greatly limits its clinical application. MicroRNAs (miRNAs) are emerged as critical mediators of cardiomyocyte injury. This work explored the function of miR-215-5p in the regulation of DOX-induced mouse HL-1 cardiomyocyte injury. An in vitro model of DOX-treated cardiotoxicity was established in HL-1 cells. Gene expression was measured by RT-qPCR. Cell viability was detected using CCK-8. Cell death and apoptosis were tested using TUNEL, flow cytometry, and caspase 3/7 activity assays. Luciferase reporter assay was used to examine the target of miR-215-5p. We found that DOX induced cardiomyocyte injury and upregulated miR-215-5p in HL-1 cells. Inhibition of miR-215-5p attenuated DOX-induced cardiomyocyte death and apoptosis in vitro. Mechanistical experiments indicated that ZEB2 was targeted by miR-215-5p. Additionally, ZEB2 expression was reduced in DOX-treated HL-1 cells. Rescue assays indicated that ZEB2 knockdown reversed the effects of miR-215-5p inhibition. In conclusion, miR-215-5p inhibition protects HL-1 cells against DOX-induced injury by upregulating ZEB2 expression.
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http://dx.doi.org/10.1097/FJC.0000000000001110DOI Listing
July 2021

Predicting Treatment Response of Neoadjuvant Chemoradiotherapy in Locally Advanced Rectal Cancer Using Amide Proton Transfer MRI Combined With Diffusion-Weighted Imaging.

Front Oncol 2021 1;11:698427. Epub 2021 Jul 1.

Department of Radiology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, China.

Objective: To evaluate amide proton weighted (APTw) MRI combined with diffusion-weighted imaging (DWI) in predicting neoadjuvant chemoradiotherapy (NCRT) response in patients with locally advanced rectal cancer (LARC).

Methods: 53 patients with LARC were enrolled in this retrospective study. MR examination including APTw MRI and DWI was performed before and after NCRT. APTw SI, ADC value, tumor size, CEA level before and after NCRT were assessed. The difference of the above parameters between before and after NCRT was calculated. The tumor regression grading (TRG) was assessed by American Joint Committee on Cancer's Cancer Staging Manual AJCC 8th score. The Shapiro-Wilk test, paired t-test and Wilcoxon Signed Ranks test, two-sample t-test, Mann-Whitney U test and multivariate analysis were used for statistical analysis.

Results: Of the 53 patients, 19 had good responses (TRG 0-1), 34 had poor responses (TRG 2-3). After NCRT, all the rectal tumors demonstrated decreased APT values, increased ADC values, reduced tumor volumes and CEA levels (all p < 0.001). Good responders demonstrated higher pre-APT values, higher Δ APT values, lower pre- ADC values and higher Δ tumor volumes than poor responders. Pre-APT combined with pre-ADC achieved the best diagnostic performance, with AUC of 0.895 (sensitivity of 85.29%, specificity of 89.47%, p < 0.001) in predicting good response to NCRT.

Conclusion: The combination of APTw and DWI may serve as a noninvasive biomarker for evaluating and identifying response to NCRT in LARC patients.
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http://dx.doi.org/10.3389/fonc.2021.698427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281887PMC
July 2021

Enzalutamide-Induced Upregulation of PCAT6 Promotes Prostate Cancer Neuroendocrine Differentiation by Regulating miR-326/HNRNPA2B1 Axis.

Front Oncol 2021 30;11:650054. Epub 2021 Jun 30.

Department of Urology, Tongji Hospital, Tongji University of Medicine, Shanghai, China.

Our previous studies have demonstrated that Enzalutamide-induced upregulation of long non-coding RNA p21 (lncRNA-p21) facilitates prostate cancer (PCa) neuroendocrine differentiation (NED). Given the important role of lncRNAs in PCa pathogenesis, and given that lots of lncRNAs are dys-regulated in neuroendocrine PCa (NEPC) patients, we next explored the biological function and underlying mechanism of lncRNA-PCAT6 (PCAT6) in mediating Enzalutamide-induced NED. The level of PCAT6 in Enzalutamide-treated PCa cells and NEPC samples were assessed using quantitative RT-PCR (qPCR). The effect of PCAT6 on PCa cell proliferation, invasion, and NED was evaluated through CCK-8, transwell, qPCR, western blot analysis, Xenograft mouse model, and lung metastasis model. We found that PCAT6 was highly expressed in NE-like cells (PC3, DU145, and NCI-H660) compared with androgen-sensitive LNCaP cells. PCAT6 was also highly expressed in NEPC tissues. Enzalutamide treatment resulted in a significant increase of PCAT6 level in a dose- and time-dependent fashion. Functionally, PCAT6 overexpression promoted NED of C4-2 cells, as evidenced by an increased expression of NE markers (NSE, ChgA, and SYP), whereas PCAT6 knockdown in NCI-H661 cells repressed NED. Furthermore, PCAT6 overexpression promoted PCa cell proliferation and invasion and . Mechanistically, PCAT6 functioned as competing endogenous (ce) RNA absorbing miR-326, thus resulting in a de-suppression of target gene. The current results demonstrate that PCAT6 acted as a tumor activator in PCa progression by sponging miR-326 and increasing Hnrnpa2b1 expression and that the PCAT6/miR-326/Hnrnpa2b1 signaling might be a new therapeutic target for PCa.
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http://dx.doi.org/10.3389/fonc.2021.650054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278330PMC
June 2021

Eight weeks of intermittent fasting versus calorie restriction does not alter eating behaviors, mood, sleep quality, quality of life and cognitive performance in women with overweight.

Nutr Res 2021 Jun 24;92:32-39. Epub 2021 Jun 24.

Adelaide Medical School, The University of Adelaide, Adelaide, South Australia, Australia; Lifelong Health Theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, South Australia, Australia. Electronic address:

Human trials that compare intermittent fasting (IF) to calorie restriction (CR) with psychological, behavioral and cognition outcomes are limited. We hypothesized that there would be no difference between CR and IF on perceived eating behaviors, mood, sleep quality, quality of life (QOL) and cognition in women with overweight and obesity. In this prespecified secondary analysis of an open-label, single center, parallel assignment, randomized controlled trial, healthy women with overweight or obesity (N = 46, mean [SD] age 50 [9] years, BMI 32.9 [4.4] kg/m), without a diagnosed eating disorder and who were randomized into 2 weight loss groups (prescribed 70% of calculated energy requirements as IF or CR) were included. Measurements were assessed in both IF and CR groups following a 12-hour overnight fast during baseline and week 8 and additionally following a 24-hour fast in the IF group only at week 8. We observed that IF produced greater weight and body fat loss than CR (P < .001). We did not detect any statistical difference between groups for the change in dietary restraint, disinhibition, hunger, mood, sleep quality, and QOL. An increase in cognitive performance was found in both IF (P = .036) and CR (P = .006) groups in one of the cognitive tasks, but there was no statistical difference between groups. Perceived eating behaviors, mood, sleep quality and cognitive performance were not changed by an acute 24-hour fast within the IF group (all P > .05). IF may be a viable alternative to CR for weight loss, in the short-term, without adversely impacting eating behaviors, mood, sleep quality, QOL or cognition in healthy women with overweight or obesity. However, larger and long term trials are required.
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http://dx.doi.org/10.1016/j.nutres.2021.06.006DOI Listing
June 2021

Regulation of the nucleic acid-sensing Toll-like receptors.

Nat Rev Immunol 2021 Jul 16. Epub 2021 Jul 16.

Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.

Many of the ligands for Toll-like receptors (TLRs) are unique to microorganisms, such that receptor activation unequivocally indicates the presence of something foreign. However, a subset of TLRs recognizes nucleic acids, which are present in both the host and foreign microorganisms. This specificity enables broad recognition by virtue of the ubiquity of nucleic acids but also introduces the possibility of self-recognition and autoinflammatory or autoimmune disease. Defining the regulatory mechanisms required to ensure proper discrimination between foreign and self-nucleic acids by TLRs is an area of intense research. Progress over the past decade has revealed a complex array of regulatory mechanisms that ensure maintenance of this delicate balance. These regulatory mechanisms can be divided into a conceptual framework with four categories: compartmentalization, ligand availability, receptor expression and signal transduction. In this Review, we discuss our current understanding of each of these layers of regulation.
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http://dx.doi.org/10.1038/s41577-021-00577-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283745PMC
July 2021

Gill oxidative damage caused by acute ammonia stress was reduced through the HIF-1α/NF-κb signaling pathway in golden pompano (Trachinotus ovatus).

Ecotoxicol Environ Saf 2021 Jul 12;222:112504. Epub 2021 Jul 12.

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, Guangdong Province, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 511458 Guangdong Province, China; Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, Guangdong Province, China; Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China; Sanya Tropical Fisheries Research Institute, Sanya, Hainan Province, China. Electronic address:

This study aimed to investigate the intoxication mechanism of golden pompano (Trachinotus ovatus) exposed to high ammonia levels and the effects on the immune and antioxidant mechanisms of gills. Juvenile golden pompano was exposed to ammonia (total ammonia: 26.9 mg/L) to induce 96 h of ammonia stress, and a 96 h recovery experiment was performed after poisoning. Then, we evaluated hematological parameters, the histological structure and the expression of related genes. In this experiment, continuous exposure to high levels of ammonia led to a significant increase in plasma alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH) levels (P < 0.05), and the levels of triiodothyronine (T3) and tetraiodothyronine (T4) were significantly reduced (P < 0.05). Moreover, the expression of antioxidant genes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and inflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) increased (P < 0.05). These results indicate that ammonia activates the active osmotic regulatory mechanism of fish gills and participates in defense and immune responses. However, with prolonged exposure to ammonia, the balance of the defense system is disrupted, leading to oxidative damage and inflammation of the gill tissue. This research not only helps elucidate the intoxication mechanism of golden pompano by ammonia at the molecular level but also provides a theoretical basis for further research on detoxification mechanisms.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112504DOI Listing
July 2021

High-efficiency plastome base-editing in rice with TAL cytosine deaminase.

Mol Plant 2021 Jul 12. Epub 2021 Jul 12.

Division of Plant Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA; Donald Danforth Plant Science Center, St. Louis, MO 63132, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2021.07.007DOI Listing
July 2021

Visualization of O/ATP cross-talk in living cells with a smart fluorescent nanoprobe.

Chem Commun (Camb) 2021 Jul 15. Epub 2021 Jul 15.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Chemical Biology and Nanomedicine (ICBN), Hunan University, Changsha, 410082, China.

Herein, we propose a dual-responsive fluorescent nanoprobe to visualize the cross-talk between O2 and adenosine triphosphate (ATP) in living cells. We hope it will be a helpful tool for the further understanding of cellular metabolism and further facilitating risk warning in the process of adaptation to consistent environmental pressures in premalignant lesions.
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http://dx.doi.org/10.1039/d1cc02644eDOI Listing
July 2021

Effects of Projections on Radiation Dose for and Image Quality of Chest Digital Radiography for Children.

Curr Med Imaging 2021 Jul 14. Epub 2021 Jul 14.

Department of Radiology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Background: Until now few studies have specially validated whether the sex, body mass index, or imaging projections of pediatric patients undergoing chest digital radiography (DR) affect the radiation dose and image quality.

Introduction: To investigate the impact of different photography positions on radiation dose for and image quality of chest DR for 3-4-year-old children.

Method: One-hundred twenty 3-4-year-old patients who required chest DR were included. The patients were divided into 3 groups, with 40 patients in each group: supine anterior-posterior projection (APP), standing APP and posterior-anterior projection (PAP). The dose area product (DAP) and entrance surface dose (ESD) values for every patient were recorded after each exposure. The visual grading analysis score (VGAS) was used to evaluate image quality.

Result: The DAP and ESD values for the standing PAP and APP groups were significantly lower than those for the supine APP group (0.19 ± 0.04 dGy cm and 0.05 ± 0.01 mGy vs 0.25 ± 0.05 dGy cm and 0.08 ± 0.01 mGy, P<0.05, respectively). Additionally, the VGAS for the standing APP group was significantly lower than those for the standing PAP and supine APP groups (28.58 ± 0.96 vs 29.08 ± 0.94 and 29.03 ± 0.80, P<0.05, respectively), whereas the pulmonary field area for the standing PAP group was significantly higher than those for the standing and supine APP groups (118.95 ± 16.81 cm vs 105.65 ± 14.76 cm and 105.24 ± 16.32 cm, P<0.05, respectively). However, there were no statistically significant differences in DAP, ESD, VGAS, pulmonary field area and body mass index between the male and female patients in the three groups (P>0.05, respectively).

Conclusion: The standing PAP should be the first projection choice for chest DR for 3-4-year-old children; compared with the supine and standing APP, the standing PAP may improve image quality and decrease the required radiation dose.
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http://dx.doi.org/10.2174/1573405617666210714120226DOI Listing
July 2021

High-quality welding of glass by a femtosecond laser assisted with silver nanofilm.

Appl Opt 2021 Jun;60(18):5360-5364

Glass products with high joint strength are highly demanded in the field of microelectromechanical system (MEMS). While the quality requirement of MEMS is getting higher and higher, much attention has been paid to further improving the welding strength of the glass. Herein, a femtosecond laser welding method assisted by silver nanofilm for quartz glass is proposed. To optimize the welding results, the influence of the laser power on the location of the heat-affected zone is studied. The effect of coated silver nanofilm at the interface of two glass substrates on femtosecond laser absorptivity is conducted. Also, the welding spot size under different irradiation periods is investigated. In addition, the welding strength with and without the silver nanofilm is measured and compared. It is demonstrated that the welding strength was increased nearly 20% on average by our proposed method compared with direct femtosecond laser welding. In addition, even at the lower laser power than the welding threshold, the welding process could be realized by the proposed method.
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http://dx.doi.org/10.1364/AO.422078DOI Listing
June 2021

Efficient Schottky Junction Construction in Metal-Organic Frameworks for Boosting H Production Activity.

Adv Sci (Weinh) 2021 Jul 7;8(13):2004456. Epub 2021 May 7.

College of Materials Science and Opto-electronic Technology, CAS Center for Excellence in Topological Quantum Computation & Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences Yanqi Lake, Huairou District Beijing 101408 P. R. China.

Manipulation of the co-catalyst plays a vital role in charge separation and reactant activation to enhance the activity of metal-organic framework-based photocatalysts. However, clarifying and controlling co-catalyst related charge transfer process and parameters are still challenging. Herein, three parameters are proposed, (the electron transfer rate from MOF to co-catalyst), (the electron transfer distance from MOF to co-catalyst), and (the electron consume rate from co-catalyst to the reactant), related to Pt on UiO-66-NH in a photocatalytic process. These parameters can be controlled by rational manipulation of the co-catalyst via three steps: i) Compositional design by partial substitution of Pt with Pd to form PtPd alloy, ii) location control by encapsulating the PtPd alloy into UiO-66-NH crystals, and iii) facet selection by exposing the encapsulated PtPd alloy (100) facets. As revealed by ultrafast transient absorption spectroscopy and first-principles simulations, the new Schottky junction (PtPd (100)@UiO-66-NH) with higher and exhibits enhanced electron-hole separation and HO activation than the traditional Pt/UiO-66-NH junction, thereby leading to a significant enhancement in the photoactivity.
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http://dx.doi.org/10.1002/advs.202004456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261486PMC
July 2021

3D printing of lubricative stiff supramolecular polymer hydrogels for meniscus replacement.

Biomater Sci 2021 Jul;9(15):5116-5126

School of Materials Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300350, China.

3D printing of a stiff and lubricative hydrogel-based meniscus substitute has been challenging since printability and stiffness compromise each other. In this work, based on an upgraded self-thickening and self-strengthening strategy, a unique multiple H-bonding monomer N-acryloylsemicarbazide (NASC) is firstly copolymerized with a super-hydrophilic monomer carboxybetaine acrylamide (CBAA) in dimethyl sulfoxide (DMSO)/H2O to form a soft poly(NASC-co-CBAA) gel, in which PCBAA serves to weaken the H-bonding interaction and avoid hydrophobic phase separation. The poly(NASC-co-CBAA) gel is then loaded with concentrated NASC and CBAA, followed by heating to form a thickening sol ink, which is printed into different objects that are further photoirradiated to initiate the copolymerization of entrapped NASC and CBAA, resulting in the formation of a high performance hydrogel with a Young's modulus of 10.98 MPa, tensile strength of 1.87 MPa and tearing energy of 5333 J m-2 after DMSO is completely replaced with water, due to the re-establishment of NASC H-bonds. Importantly, PCBAA affords high lubricity in printed hydrogels. The printed PNASC-PCBAA meniscus substitute can substitute rabbit's native meniscus and ameliorate the cartilage surface wear within a set 12-week time window, portending great potential as a meniscal substitute and other soft-supporting tissue scaffolds.
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http://dx.doi.org/10.1039/d1bm00836fDOI Listing
July 2021

Development and Evaluation of a Leukemia Diagnosis System Using Deep Learning in Real Clinical Scenarios.

Front Pediatr 2021 24;9:693676. Epub 2021 Jun 24.

Pediatric AI Clinical Application and Research Center, Shanghai Children's Medical Center, Shanghai, China.

Leukemia is the most common malignancy affecting children. The morphologic analysis of bone marrow smears is an important initial step for diagnosis. Recent publications demonstrated that artificial intelligence is able to classify blood cells but a long way from clinical use. A total of 1,732 bone marrow images were used for the training of a convolutional neural network (CNN). New techniques of deep learning were integrated and an end-to-end leukemia diagnosis system was developed by using raw images without pre-processing. The system creatively imitated the workflow of a hematologist by detecting and excluding uncountable and crushed cells, then classifying and counting the remain cells to make a diagnosis. The performance of the CNN in classifying WBCs achieved an accuracy of 82.93%, precision of 86.07% and F1 score of 82.02%. And the performance in diagnosing acute lymphoid leukemia achieved an accuracy of 89%, sensitivity of 86% and specificity of 95%. The system also performs well at detecting the bone marrow metastasis of lymphoma and neuroblastoma, achieving an average accuracy of 82.93%. This is the first study which included a wider variety of cell types in leukemia diagnosis, and achieved a relatively high performance in real clinical scenarios.
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http://dx.doi.org/10.3389/fped.2021.693676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264256PMC
June 2021

MDM2 Binding Protein Induces the Resistance of Hepatocellular Carcinoma Cells to Molecular Targeting Agents Enhancing the Transcription Factor Activity of the Pregnane X Receptor.

Front Oncol 2021 24;11:715193. Epub 2021 Jun 24.

Endoscopy Center, Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

The MDM2 binding protein (MTBP) has been considered an important regulator of human malignancies. In this study, we demonstrate that the high level of MTBP's endogenous expression is correlated with poor prognosis of advanced hepatocellular carcinoma (HCC) patients who received sorafenib. MTBP interacted with the Pregnane X receptor (PXR) and enhanced the transcription factor activity of PXR. Moreover, MTBP enhanced the accumulation of PXR in HCC cells' nuclear and the recruitment of PXR to its downstream gene's () promoter region. Mechanically, the knockdown of MTBP in MHCC97-H cells with high levels of MTBP decelerated the clearance or metabolism of sorafenib in HCC cells and led to the resistance of HCC cells to sorafenib. Whereas overexpression of MTBP in in MHCC97-L cells with low levels of MTBP showed the opposite trend. By establishing the interaction between MTBP and PXR, our results indicate that MTBP could function as a co-activator of PXR and could be a promising therapeutic target to enhance the sensitivity of HCC cells to molecular targeting agents.
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http://dx.doi.org/10.3389/fonc.2021.715193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264664PMC
June 2021

Wheat Rhizosphere Metagenome Reveals Newfound Potential Soil Zn-Mobilizing Bacteria Contributing to Cultivars' Variation in Grain Zn Concentration.

Front Microbiol 2021 23;12:689855. Epub 2021 Jun 23.

State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, China.

An effective solution to global human zinc (Zn) deficiency is Zn biofortification of staple food crops, which has been hindered by the low available Zn in calcareous soils worldwide. Many culturable soil microbes have been reported to increase Zn availability in the laboratory, while the status of these microbes in fields and whether there are unculturable Zn-mobilizing microbes remain unexplored. Here, we use the culture-independent metagenomic sequencing to investigate the rhizosphere microbiome of three high-Zn (HZn) and three low-Zn (LZn) wheat cultivars in a field experiment with calcareous soils. The average grain Zn concentration of HZn was higher than the Zn biofortification target 40 mg kg, while that of LZn was lower than 40 mg kg. Metagenomic sequencing and analysis showed large microbiome difference between wheat rhizosphere and bulk soil but small difference between HZn and LZn. Most of the rhizosphere-enriched microbes in HZn and LZn were in common, including many of the previously reported soil Zn-mobilizing microbes. Notably, 30 of the 32 rhizosphere-enriched species exhibiting different abundances between HZn and LZn possess the functional genes involved in soil Zn mobilization, especially the synthesis and exudation of organic acids and siderophores. Most of the abundant potential Zn-mobilizing species were positively correlated with grain Zn concentration and formed a module with strong interspecies relations in the co-occurrence network of abundant rhizosphere-enriched microbes. The potential Zn-mobilizing species, especially and , may contribute to the cultivars' variation in grain Zn concentration, and they deserve further investigation in future studies on Zn biofortification.
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http://dx.doi.org/10.3389/fmicb.2021.689855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261137PMC
June 2021

Analysis of risk factors for C5 nerve root paralysis after posterior cervical decompression.

BMC Musculoskelet Disord 2021 Jul 10;22(1):614. Epub 2021 Jul 10.

Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, 266071, Qingdao, Shandong Province, China.

Background: C5 nerve root paralysis is a nonnegligible complication after posterior cervical spine surgery (PCSS). The cause of its occurrence remains controversial. The purpose of this study was to analyse the incidence of and risk factors for C5 nerve root paralysis after posterior cervical decompression.

Methods: We retrospectively analysed the clinical data of 640 patients who underwent PCSS in the Department of Orthopaedics, Affiliated Hospital of Qingdao University from September 2013 to September 2019. According to the status of C5 nerve root paralysis after surgery, all patients were divided into paralysis and normal groups. Univariate and multivariate analyses were used to determine the independent risk factors for C5 nerve root paralysis. A receiver operating characteristic (ROC) curve was used to demonstrate the discrimination of all independent risk factors.

Results: Multivariate logistic regression analysis revealed that male sex, preoperative cervical spine curvature, posterior longitudinal ligament ossification, and preoperative C4/5 spinal cord hyperintensity were independent risk factors for paralysis, whereas the width of the intervertebral foramina was an independent protective factor for paralysis. The area under the curve (AUC) values of the T2 signal change at C4-C5, sex, cervical foramina width, curvature and posterior longitudinal ligament ossification were 0.706, 0.633, 0.617, 0.637, and 0.569, respectively.

Conclusions: Male patients with C4-C5 intervertebral foramina stenosis, preoperative C4-C5 spinal cord T2 high signal, combined with OPLL, and higher preoperative cervical spine curvature are more likely to develop C5 nerve root paralysis after surgery. Among the above five risk factors, T2 hyperintensity change in C4-C5 exhibits the highest correlation with C5 paralysis and strong diagnostic power. It seems necessary to inform patients who have had cervical spine T2 hyperintensity before surgery of C5 nerve root paralysis after surgery, especially those with altered spinal cord T2 signals in the C4-C5 segment.
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http://dx.doi.org/10.1186/s12891-021-04434-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272892PMC
July 2021

Triptolide dose-dependently improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS mice.

Biomed Pharmacother 2021 Jul 24;139:111569. Epub 2021 May 24.

Department of Intensive Care Unit, Guizhou Medical University Affiliated Hospital, Guiyang 550001, China. Electronic address:

Background: Alveolar hypercoagulation and fibrinolysis inhibition were associated with the refractory hypoxemia and the high mortality in patient with acute respiratory distress syndrome (ARDS), and NF-κB pathway was confirmed to contribute to the process. Triptolide (TP) significantly inhibited NF-κB pathway and thus depressed accessive inflammatory response in ARDS. We speculate that TP could improve alveolar hypercoagulation and fibrinolytic inhibition in LPS-induced ARDS via NF-κB inactivation.

Purpose: The aim of this experiment was to explore the efficacy and potential mechanism of TP on alveolar hypercoagulation and fibrinolysis inhibition in LPS-induced ARDS in mice.

Methods: 50 μl of LPS (5 mg/ml) was inhalationally given to C57BL/6 mice to set up ARDS model. Male mice were randomly accepted with LPS, LPS + TP (1 μg/kg, 10 μg/kg, 50 μg/kg respectively), or with NEMO Binding domain peptide (NBD), an inhibitor of NF-κB. TP (1 μg/kg, 10 μg/kg, 50 μg/kg) were intraperitoneally injected or 10 μg/50 μl of NBD solution were inhaled 30 min before LPS inhalation. A same volume of normal saline (NS) substituted for TP in mice in control. The endpoint of experiment was at 8 hours after LPS stimulation. Pulmonary tissues were taken for hematoxylin-eosin (HE) staining, wet / dry ratio and for lung injury scores (LIS). Tissue factor (TF) and plasminogen activator inhibitor (PAI)-1 in lung tissue were detected by Western-blotting and by quantitative Real-time PCR(qPCR) respectively. Concentrations of TF, PAI-1, thrombin-antithrombin complex (TAT), procollagen peptide type Ⅲ (PⅢP) and activated protein C (APC) in bronchoalveolar lavage fluid (BALF) were measured by ELISA. NF-κB activation and p65-DNA binding activity in pulmonary tissue were simultaneously determined.

Results: LPS stimulation resulted in pulmonary edema, neutrophils infiltration, obvious alveolar collapse, interstitial congestion, with high LIS, which were all dose-dependently ameliorated by Triptolide. LPS also dramatically promoted the expressions of TF and PAI-1 either in mRNA or in protein in lung tissue, and significantly stimulated the secretions of TF, PAI-1, TAT, PⅢP but inhibited APC production in BALF, which were all reversed by triptolide treatment in dose-dependent manner. TP dose-dependently inhibited the activation of NF-κB pathway induced by LPS, indicated by the changes of phosphorylations of p65 (p-p65), p-IKKα/β and p-IκBα, and weakened p65-DNA binding activity. TP and NBD had same efficacies either on alveolar hypercoagulation and fibrinolysis inhibition or on NF-κB signalling pathway in ARDS mice.

Conclusions: TP dose-dependently improves alveolar hypercoagulation and fibrinolysis inhibition in ARDS mice through inhibiting NF-κB signaling pathway. Our data demonstrate that TP is expected to be an effective selection in ARDS.
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http://dx.doi.org/10.1016/j.biopha.2021.111569DOI Listing
July 2021
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