Publications by authors named "Bo Lin"

267 Publications

Comprehensive co-expression analysis reveals TMC8 as a prognostic immune-associated gene in head and neck squamous cancer.

Oncol Lett 2021 Jul 27;22(1):498. Epub 2021 Apr 27.

Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

The occurrence and prognosis of head and neck squamous cell cancer (HNSC) is closely associated with human papillomavirus (HPV) infection. Transmembrane channel-like 8 (TMC8) is a key gene affecting the susceptibility of HPV and that plays an important role in T cell regulation. However, the mechanism by which TMC8 affects T cells and whether it further affects the prognosis of patients with HNSC remains unclear. In the present study, oral cancer cell lines and independent tumor specimens were used to detect TMC8 expression in HNSC. Differential expression of TMC8, methylation status, function and associated signaling pathways were further analyzed. Then, multiple databases were cross-analyzed for the relationship of TMC8 with immune cell infiltration and its impact on the prognosis of numerous types of cancer. The results showed that TMC8 was upregulated in HNSC and high expression was predictive of an improved prognosis. Furthermore, TMC8 was concentrated in multiple immune-associated signaling pathways and the expression of TMC8 was associated with the infiltration of CD4 T cells and their subsets, including CD8 T cells, B cells and macrophages, suggesting that TMC8 may play an anti-HPV role by regulating CD4 T cells. Thus, TMC8 plays an anti-HPV role by regulating the infiltration level of CD4 T cells, and could therefore be used as a potential prognostic marker for patients with HNSC.
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http://dx.doi.org/10.3892/ol.2021.12759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108259PMC
July 2021

AFP-Inhibiting Fragments for Drug Delivery: The Promise and Challenges of Targeting Therapeutics to Cancers.

Front Cell Dev Biol 2021 8;9:635476. Epub 2021 Apr 8.

Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China.

Alpha fetoprotein (AFP) plays a key role in stimulating the growth, metastasis and drug resistance of hepatocellular carcinoma (HCC). AFP is an important target molecule in the treatment of HCC. The application of AFP-derived peptides, AFP fragments and recombinant AFP (AFP-inhibiting fragments, AIFs) to inhibit the binding of AFP to intracellular proteins or its receptors is the basis of a new strategy for the treatment of HCC and other cancers. In addition, AIFs can be combined with drugs and delivery agents to target treatments to cancer. AIFs conjugated to anticancer drugs not only destroy cancer cells with these drugs but also activate immune cells to kill cancer cells. Furthermore, AIF delivery of drugs relieves immunosuppression and enhances chemotherapy effects. The synergism of immunotherapy and targeted chemotherapy is expected to play an important role in enhancing the treatment effect of patients with cancer. AIF delivery of drugs will be an available strategy for the targeted treatment of cancer in the future.
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http://dx.doi.org/10.3389/fcell.2021.635476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061420PMC
April 2021

Apolipoprotein A1 is negatively associated with male papillary thyroid cancer patients: a cross-sectional study of single academic center in China.

BMC Endocr Disord 2021 Apr 14;21(1):69. Epub 2021 Apr 14.

Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, Zhongshan II Road, No 58, Guangzhou, 510000, China.

Background: Papillary thyroid cancer (PTC) is the most common type of thyroid cancer and the incidence of PTC has continued to increase over the past decades. Many studies have shown that obesity is an independent risk factor for PTC and obese PTC patients tend to have a relative larger tumor size and higher grade of tumor stage. Obesity is associated with disordered lipid metabolism and the relationship between serum lipids and PTC remains unclear. Therefore, this study aimed to investigate the association between serum lipid level and PTC.

Methods: We retrospectively analyzed 1018 PTC patients diagnosed and treated in our hospital, all these cases were first diagnosed with PTC and had complete clinical information including ultrasound reports before surgery, serum lipid (CHOL, TG, HDL-c, LDL-c, Apo-A1, Apo-B, Apo-E) results, surgical records and pathological reports.

Results: None of these lipid markers were associated with tumor size in the whole cohort and in the female group. In the male group, on crude analysis, Apo-A1 showed a marginally association with tumor size, [OR = 0.158 (0.021-1.777)], p = 0.072. After adjusting for age and multifocality, Apo-A1 showed a significant association with tumor size [OR = 0.126 (0.016-0.974)], p = 0.047. This association become more apparent in a young male subgroup, [OR = 0.051 (0.005-0.497)], p = 0.009. CHOL, TG, HDL-c, LDL-c, Apo-B, Apo-E did not show significant association with tumor size. As for LNM, neither in the male group nor in the female group were found to be associated with any serum lipid biomarkers.

Conclusion: As PTC incidences continues to increase, our findings demonstrated a negatively association between PTC and apoA-1 in male PTC patients, which may contribute to further investigation concerning diagnosing and preventing this most common type of thyroid cancer.
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http://dx.doi.org/10.1186/s12902-021-00714-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048163PMC
April 2021

Investigating the mechanisms of Modified Xiaoyaosan (tiaogan-liqi prescription) in suppressing the progression of atherosclerosis, by means of integrative pharmacology and experimental validation.

Aging (Albany NY) 2021 Apr 4;13(8):11411-11432. Epub 2021 Apr 4.

Department of Cardiovascular Disease, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.

Atherosclerosis (AS)-related diseases remain among the leading causes of death worldwide. Modified Xiaoyaosan (also called Tiaogan-Liqi prescription, TGLQ), a traditional Chinese medical formulation, has been widely applied in the treatment of AS-related diseases. The aim of this study was to investigate the underlying pharmacological mechanisms of TGLQ in acting on AS. A total of 548 chemical compounds contained in TGLQ, and 969 putative targets, were collected from the Computation Platform for Integrative Pharmacology of Traditional Chinese Medicine, while 1005 therapeutic targets for the treatment of AS were obtained from the DisGeNET, TTD and CTD databases. Moreover, the 63 key targets were screened by the intersection of the targets above, and by network topological analysis. Further functional enrichment analysis showed that the key targets were significantly associated with regulation of the immune system and inflammation, improvement of lipid and glucose metabolism, regulation of the neuroendocrine system and anti-thrombosis effect. The experiments confirmed that TGLQ could reduce plasma lipid profiles and plasma inflammatory cytokines, and also inhibit AS plaque formation, within the AS model ApoE-/- mice. The experiments validated the hypothesis that TGLQ could significantly reduce intracellular lipid accumulation, suppress the production of inflammatory cytokines of macrophages induced by oxidized-LDL, and inhibit the protein expression of heat shock protein 90 and toll-like receptor 4. This study identified a list of key targets of TGLQ in the treatment of AS by applying an integrative pharmacology approach, which was validated by and experimentation.
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http://dx.doi.org/10.18632/aging.202832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109114PMC
April 2021

Gallbladder bleeding along with cholecystocolonic fistula: a case report.

Gastroenterol Rep (Oxf) 2021 Jan 20;9(1):81-84. Epub 2020 Jul 20.

Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu, Sichuan, P. R. China.

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http://dx.doi.org/10.1093/gastro/goaa034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962734PMC
January 2021

Upregulation of CPNE3 suppresses invasion, migration and proliferation of glioblastoma cells through FAK pathway inactivation.

J Mol Histol 2021 Mar 16. Epub 2021 Mar 16.

Department of Neurosurgery, Chongqing General Hospital, University of Chinese Academy of Science, 118 Xingguang Avenue, Liangjiang New Area, Chongqing, 400016, China.

Glioblastoma (GBM) is a deadly brain tumor with a bleak prognosis. In recent years, the copine III (CPNE3) protein was discovered to be associated to metastasis across various types of malignancies. Nevertheless, its function has not been well documented in glioma. This study characterizes CPNE3 expression in GBM along with its impact and underlying molecular mechanism with regards to cellular migration, invasion and proliferation. Immunohistochemistry was used to characterizes CPNE3 expression in the glioma tissues. Then, knockdown of CPNE3 expression was used to analyze the role of CPNE3 in GBM cell viability, migration, invasion. Western blot analysis was performed to measure the protein levels of FAK signaling pathway. We found that GBM tissues had higher CPNE3 expressions as compared to those in normal brain tissues. CPNE3 silencing in GBM cells impaired the migratory, invasive and proliferative abilities of GBM cells that can be attributed to inactivation of the FAK signaling pathway. Collectively, these findings highlight the role of CPNE3 as a new biomarker, offering deeper insights into its carcinogenic role in GBM.
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http://dx.doi.org/10.1007/s10735-021-09966-0DOI Listing
March 2021

MiR-105-3p acts as an oncogene to promote the proliferation and metastasis of breast cancer cells by targeting GOLIM4.

BMC Cancer 2021 Mar 15;21(1):275. Epub 2021 Mar 15.

Department of Pathology, Huai'an Key Laboratory of Gastric Cancer, Jiangsu College of Nursing, No. 9 Keji Road, Huai'an, Jiangsu, 223001, P.R. China.

Background: Dysregulated miRNAs are involved in carcinogenesis of the breast and may be used as prognostic biomarkers and therapeutic targets during the cancer process. The purpose of this study was to explore the effect of miR-105-3p on the tumourigenicity of breast cancer and its underlying molecular mechanisms.

Methods: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of miR-105-3p in breast cancer tissues and cell lines. The impacts of miR-105-3p on the proliferation, migration, invasion and apoptosis of human breast cancer cells (MCF-7 and ZR-75-30) were evaluated by CCK-8 assays, Transwell chamber assays, TUNEL assays and western blot analyses. In addition, bioinformatics and luciferase reporter assays were used to determine the target genes of miR-105-3p.

Results: The expression of miR-105-3p was elevated in breast cancer tissues and increased with tumour severity. Downregulation of miR-105-3p could inhibit cell proliferation, suppress cell migration/invasion, and promote cell apoptosis in MCF-7 and ZR-75-30 cells. Furthermore, Golgi integral membrane protein 4 (GOLIM4) was identified as the direct target gene of miR-105-3p by bioinformatics and luciferase reporter assays. In addition, silencing GOLIM4 restored the anti-breast cancer effects induced by miR-105-3p downregulation.

Conclusions: MiR-105-3p acts as an oncogene to promote the proliferation and metastasis of breast cancer cells by targeting GOLIM4, which provides a new target for the prevention and treatment of breast cancer.
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http://dx.doi.org/10.1186/s12885-021-07909-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962220PMC
March 2021

Use of machine learning to efficiently predict the confinement loss in anti-resonant hollow-core fiber.

Opt Lett 2021 Mar;46(6):1454-1457

The fundamental mode confinement loss (CL) of anti-resonant hollow-core fiber (ARF) is efficiently predicted by a classification task of machine learning. The structure-parameter vector is utilized to define the sample space of ARFs. The CL of labeled samples at 1550 nm is numerically calculated via the finite element method (FEM). The magnitude of CL is obtained by a classification task via a decision tree and -nearest neighbors algorithms with the training and test sets generated by 290700 and 32300 labeled samples. The test accuracy, confusion matrices, and the receiver operating characteristic curves have shown that our proposed method is effective for predicting the magnitude of CL with a short computation runtime compared to FEM simulation. The feasibility of predicting other performance parameters by the extension of our method, as well as its ability to generalize outside the tested sample space, is also discussed. It is likely that the proposed sample definition and the use of a classification approach can be adopted for design application beyond efficient prediction of ARF CL and inspire artificial intelligence and data-driven-based research of photonic structures.
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http://dx.doi.org/10.1364/OL.422511DOI Listing
March 2021

Alpha-Fetoprotein Binding Mucin and Scavenger Receptors: An Available Bio-Target for Treating Cancer.

Front Oncol 2021 25;11:625936. Epub 2021 Feb 25.

Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China.

Alpha-fetoprotein (AFP) entrance into cancer cells is mediated by AFP receptors (AFPRs) and exerts malignant effects. Therefore, understanding the structure of AFPRs will facilitate the development of rational approaches for vaccine design, drug delivery, antagonizing immune suppression and diagnostic imaging to treat cancer effectively. Throughout the last three decades, the identification of universal receptors for AFP has failed due to their complex carbohydrate polymer structures. Here, we focused on the two types of binding proteins or receptors that may serve as AFPRs, namely, the A) mucin receptors family, and B) the scavenger family. We presented an informative review with detailed descriptions of the signal transduction, cross-talk, and interplay of various transcription factors which highlight the downstream events following AFP binding to mucin or scavenger receptors. We mainly explored the underlying mechanisms involved mucin or scavenger receptors that interact with AFP, provide more evidence to support these receptors as tumor AFPRs, and establish a theoretical basis for targeting therapy of cancer.
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http://dx.doi.org/10.3389/fonc.2021.625936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947232PMC
February 2021

Administration of high titer convalescent anti-SARS-CoV-2 plasma: From donor selection to monitoring recipient outcomes.

Hum Immunol 2021 Apr 16;82(4):255-263. Epub 2021 Feb 16.

Department of Medicine, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA. Electronic address:

Early in the SARS-CoV-2 pandemic, convalescent plasma (CP) therapy was proposed as a treatment for severely ill patients. We conducted a CP treatment protocol under the Mayo Clinic Extended Access Program at University Hospital Brooklyn (UHB). Potential donors were screened with a lateral flow assay (LFA) for IgM and IgG antibodies against the SARS-CoV-2 S1 receptor-binding domain (RBD). Volunteers that were LFA positive were tested with an ELISA to measure IgG titers against the RBD. Subjects with titers of at least 1:1024 were selected to donate. Most donors with positive LFA had acceptable titers and were eligible to donate. Out of 171 volunteers, only 65 tested positive in the LFA (38.0%), and 55 (32.2%) had titers of at least 1:1024. Before our donation program started, 31 CP units were procured from the New York Blood Center (NYBC). Among the 31 CP units that were obtained from the NYBC, 25 units (80.6%) were positive in the LFA but only 12 units (38.7%) had titers of at least 1:1024. CP was administered to 28 hospitalized COVID-19 patients. Patients who received low titer CP, high titer CP and patients who did not receive CP were followed for 45 days after presentation. Severe adverse events were not associated with CP transfusion. Death was a less frequent outcome for patients that received high titer CP (>1:1024) 38.6% mortality, than patients that received low titer CP (≤1:1024) 77.8% mortality.
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http://dx.doi.org/10.1016/j.humimm.2021.02.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885628PMC
April 2021

Experimental and numerical perspective on the fire performance of MXene/Chitosan/Phytic acid coated flexible polyurethane foam.

Sci Rep 2021 Feb 25;11(1):4684. Epub 2021 Feb 25.

School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.

Recent discoveries of two-dimensional transitional metal based materials have emerged as an excellent candidate for fabricating nanostructured flame-retardants. Herein, we report an eco-friendly flame-retardant for flexible polyurethane foam (PUF), which is synthesised by hybridising MXene (Ti[Formula: see text]) with biomass materials including phytic acid (PA), casein, pectin, and chitosan (CH). Results show that coating PUFs with 3 layers of CH/PA/Ti[Formula: see text] via layer-by-layer approach reduces the peak heat release and total smoke release by 51.1% and 84.8%, respectively. These exceptional improvements exceed those achieved by a CH/Ti[Formula: see text] coating. To further understand the fundamental flame and smoke reduction phenomena, a pyrolysis model with surface regression was developed to simulate the flame propagation and char layer. A genetic algorithm was utilised to determine optimum parameters describing the thermal degradation rate. The superior flame-retardancy of CH/PA/Ti[Formula: see text] was originated from the shielding and charring effects of the hybrid MXene with biomass materials containing aromatic rings, phenolic and phosphorous compounds.
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http://dx.doi.org/10.1038/s41598-021-84083-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907131PMC
February 2021

Comprehensive analysis of macrophage-related multigene signature in the tumor microenvironment of head and neck squamous cancer.

Aging (Albany NY) 2021 02 11;13(4):5718-5747. Epub 2021 Feb 11.

Stomatological Center, Peking University Shenzhen Hospital, Shenzhen, Guangdong, China.

Macrophages are among the most abundant cells of the tumor microenvironment in head and neck squamous cancer (HNSC). Although the marker gene sets of macrophages have been found, the mechanism by which they affect macrophages and whether they further predict the clinical outcome is unclear. In this study, a univariate COX analysis and a random forest algorithm were used to construct a prognostic model. Differential expression of the key gene, methylation status, function, and signaling pathways were further analyzed. We cross-analyzed multiple databases to detect the relationship between the most critical gene and the infiltration of multiple immune cells, as well as its impact on the prognosis of pan-cancer. FANCE is recognized as hub gene by different algorithms. It was overexpressed in HNSC, and high expression was predictive of better prognosis. It might promote apoptosis through the Wnt/β-catenin pathway. The expression of FANCE is inversely proportional to the infiltration of CD4 + T cells and their subsets, tumor-associated macrophages (TAMs), M2 macrophages, but positively co-expressed with M1 macrophages. In summary, FANCE was identified as the hub gene from the macrophage marker gene set, and it may improve the prognosis of HNSC patients by inhibiting lymphocytes and tumor-associated macrophages infiltration.
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http://dx.doi.org/10.18632/aging.202499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950226PMC
February 2021

Deep learning for automatically predicting early haematoma expansion in Chinese patients.

Stroke Vasc Neurol 2021 Feb 1. Epub 2021 Feb 1.

Department of Neurology, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou, China

Background And Purpose: Early haematoma expansion is determinative in predicting outcome of intracerebral haemorrhage (ICH) patients. The aims of this study are to develop a novel prediction model for haematoma expansion by applying deep learning model and validate its prediction accuracy.

Methods: Data of this study were obtained from a prospectively enrolled cohort of patients with primary supratentorial ICH from our centre. We developed a deep learning model to predict haematoma expansion and compared its performance with conventional non-contrast CT (NCCT) markers. To evaluate the predictability of this model, it was also compared with a logistic regression model based on haematoma volume or the BAT score.

Results: A total of 266 patients were finally included for analysis, and 74 (27.8%) of them experienced early haematoma expansion. The deep learning model exhibited highest C statistic as 0.80, compared with 0.64, 0.65, 0.51, 0.58 and 0.55 for hypodensities, black hole sign, blend sign, fluid level and irregular shape, respectively. While the C statistics for swirl sign (0.70; p=0.211) and heterogenous density (0.70; p=0.141) were not significantly higher than that of the deep learning model. Moreover, the predictive value for the deep learning model was significantly superior to that of the logistic model of haematoma volume (0.62; p=0.042) and the BAT score (0.65; p=0.042).

Conclusions: Compared with the conventional NCCT markers and BAT predictive model, the deep learning algorithm showed superiority for predicting early haematoma expansion in ICH patients.
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http://dx.doi.org/10.1136/svn-2020-000647DOI Listing
February 2021

Spatial heterogeneity of tree diversity response to climate warming in montane forests.

Ecol Evol 2021 Jan 28;11(2):931-941. Epub 2020 Dec 28.

Sichuan Forestry and Grassland Research and Planning Institute Chengdu China.

Many studies reported biotic change along a continental warming gradient. However, the temporal and spatial change of tree diversity and their sensitivity to climate warming might differ from region to region. Understanding of the variation among studies with regard to the magnitude of such biotic changes is minimal, especially in montane ecosystems. Our aim is to better understand changes in spatial heterogeneity and temporal dynamics of mountain tree communities under climate warming over the past four decades. In 2017, we resurveyed and recorded all tree species from 107 long-term monitoring plots that were first studied between 1974 and 1976. These plots were located in montane forests in the Giant Panda National Park (GPNP), China. Our results showed that spatial differences were found in tree species diversity changes response to mean annual temperature change over the past four decades. Tree species richness increased significantly under climate warming in Minshan (MS) and Xiaoxiangling (XXL) with higher warming rate than Qionglai (QLS) and Liangshan (LS). The trees species diversity in MS and XXL were more sensitive to climatic warming. MS and XXL should receive priority protection in the next conservation plan of the GPNP. The GPNP should avoid taking a "one-size-fits-all" approach for diversity conservation due to spatial heterogeneity in plant community dynamics.
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http://dx.doi.org/10.1002/ece3.7106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820142PMC
January 2021

A Fbxo48 inhibitor prevents pAMPKα degradation and ameliorates insulin resistance.

Nat Chem Biol 2021 03 25;17(3):298-306. Epub 2021 Jan 25.

Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA.

The adenosine monophosphate (AMP)-activated protein kinase (Ampk) is a central regulator of metabolic pathways, and increasing Ampk activity has been considered to be an attractive therapeutic target. Here, we have identified an orphan ubiquitin E3 ligase subunit protein, Fbxo48, that targets the active, phosphorylated Ampkα (pAmpkα) for polyubiquitylation and proteasomal degradation. We have generated a novel Fbxo48 inhibitory compound, BC1618, whose potency in stimulating Ampk-dependent signaling greatly exceeds 5-aminoimidazole-4-carboxamide-1-β-ribofuranoside (AICAR) or metformin. This compound increases the biological activity of Ampk not by stimulating the activation of Ampk, but rather by preventing activated pAmpkα from Fbxo48-mediated degradation. We demonstrate that, consistent with augmenting Ampk activity, BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity in high-fat-diet-induced obese mice. Hence, we provide a unique bioactive compound that inhibits pAmpkα disposal. Together, these results define a new pathway regulating Ampk biological activity and demonstrate the potential utility of modulating this pathway for therapeutic benefit.
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http://dx.doi.org/10.1038/s41589-020-00723-0DOI Listing
March 2021

Synthesis of Sulfimides and Allyl-(thio)amides by Ru(II)-Catalyzed Nitrene Transfer Reactions of Acyloxyamides.

Org Lett 2021 Feb 11;23(3):819-825. Epub 2021 Jan 11.

College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China.

The acyloxyamides were employed as effective acyl nitrene precursors in reactions with thioethers under the catalysis of a commercially available Ru(II) complex, from which a variety of sulfimides were synthesized efficiently and mildly. If an allyl group is contained in the thioether precursor, the [2,3]-sigmatropic rearrangement of the sulfimide occurs simultaneously and the allyl--(thio)amides were obtained as the final products. Preliminary mechanistic studies indicated that the Ru-nitrenoid species should be a key intermediate in the transformation.
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http://dx.doi.org/10.1021/acs.orglett.0c04043DOI Listing
February 2021

MiR-16-5p suppresses myofibroblast activation in systemic sclerosis by inhibiting NOTCH signaling.

Aging (Albany NY) 2020 12 19;13(2):2640-2654. Epub 2020 Dec 19.

Department of Rheumatology and Immunology, The Second Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan Province, China.

Systemic sclerosis (SSc) is a prototypic fibrotic disease characterized by localized or diffuse skin thickening and fibrosis. Tissue fibrosis is driven by myofibroblasts, and factors affecting myofibroblast activation may also be involved in the development of SSc. In this study, we examined molecular mechanisms underlying SSc by focusing on myofibroblast activation processes. Bioinformatics analysis conducted to identify differentially expressed miRNAs (DEMs) and genes (DEGs) revealed that microRNA-16-5p (miR-16-5p) was downregulated and NOTCH2 was upregulated in SSc patients. experiments confirmed that miR-16-5p was able to bind directly to NOTCH2 and inhibit myofibroblast activation. Moreover, miR-16-5p-dependent inhibition of NOTCH2 decreased collagen and α-SMA expression. MiR-16-5p downregulation and NOTCH2 upregulation was also confirmed in SSc patients, and NOTCH2 activation promoted fibrosis progression . These results indicate that miR-16-5p suppresses myofibroblast activation by suppressing NOTCH signaling.
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http://dx.doi.org/10.18632/aging.202308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880343PMC
December 2020

[Absorption mechanism of dragon's blood phenolic extracts in Caco-2 cells].

Zhongguo Zhong Yao Za Zhi 2020 Oct;45(20):4889-4895

Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029, China.

The purpose of this study was to study the absorption characteristics of eight main components from dragon's blood phenolic extracts in Caco-2 cells based on the humancolon cancer cell Caco-2 model, and to clarify the oral absorption mechanism of such phenolic extracts. UPLC-MS/MS was used in this study to determine the content of 8 active ingredients including thevetiaflavone, 7,4'-dihydroxyflavone, 7,4'-dihydroxy-5-methoxyhomoisoflavanone, 7,4'-dihydroxyhomoisoflavanone, loureirin C, loureirin A, loureirin B and pterostilbene from dragon's blood phenolic extracts, and Caco-2 cells were used to investigate the effects of incubation time, concentration, temperature, P-gp inhibitor, MRP inhibitor, OCTN1 inhibitor and OCTN2 inhibitor on the absorption of each component. In addition, the transport experiment was conducted to measure the apparent permeability coefficient P_(app) and transport rate of the eight main components to predict the oral absorption mechanism of dragon's blood phenolic extracts. The experimental results showed that the cell uptake of the eight main components in dragon's blood phenolic extracts was time-dependent and concentration dependent, and the uptake of each component did not need to consume energy, which was consistent with the passive diffusion process. P-gp inhibitor, MRP inhibitor and OCTN1 inhibitor had no effect on the cell uptake of each component, only the addition of OCTN2 inhibitor significantly reduced the uptake of pterostilbene(P<0.05). In the transport results, the ER values of the outflow rates of the eight components were all less than 1.5. The above results show that the absorption mechanism of the eight components in Draconis resina phenolic extract may be passive diffusion, and pterostilbene may be the substrate of OCTN2.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200914.301DOI Listing
October 2020

The Current Landscape of Clinical Studies Focusing on Thyroid Cancer: A Comprehensive Analysis of Study Characteristics and Their Publication Status.

Front Endocrinol (Lausanne) 2020 20;11:575799. Epub 2020 Nov 20.

Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: A better understanding of the current characteristics of clinical trials on thyroid cancer (TC) is important to improve trial designs and identify neglected areas of research. However, there is a lack of a thorough understanding of the clinical studies on TC. Therefore, this study aimed to present a comprehensive overview of clinical trials on TC based on the ClinicalTrials.gov database and evaluate their publication status.

Methods: We searched for TC-related clinical studies registered in the ClinicalTrials.gov database before December 2018 by using the keyword "thyroid cancer" and assessed the characteristics of the included trials. We searched the publication status of primary completed studies in PubMed and Google Scholar.

Results: A total of 450 studies were identified for analysis, including 333 (74.0%) interventional studies and 117 (26.0%) observational studies. Interventional studies about TC were commonly non-randomized (67.6%), single-arm (55.6%), single-center (76.3%), and early-phase (60.0%) trials. The major category for which studies were performed was for target drug-related therapy (53.6%). In addition, 57.0% of the primary completed interventional studies were published. The published studies were more commonly primary completed studies after 2010 and used randomization and were less commonly designed as single-arm studies and were conducted in the USA/Canada, compared to non-published studies ( < 0.05 for all). The median time from primary completion to publication was 46.5 months, and the time decreased to 36.5 months after 2010. Studies conducted in the USA/Canada [odds ratio (OR) = 9.43, = 0.020] and multi-center studies (OR = 6.55,  = 0.021) significantly increased the potential of publication in high-impact journals.

Conclusions: High-quality, randomized phase 3 trials regarding TC are still insufficient. Therefore, more efforts are needed to improve the treatment of poor prognostic TC and timely publication.
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http://dx.doi.org/10.3389/fendo.2020.575799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714929PMC
November 2020

Correlative analysis between cytotoxic T lymphocyte antigen 4 genetic polymorphisms and head and neck cancer susceptibility: A protocol for systematic review and meta-analysis.

Authors:
Bo Lin Ling Wang

Medicine (Baltimore) 2020 Dec;99(50):e23519

School of Nursing, Wannan Medical College, Wuhu, Anhui Province, China.

Background: Previous published studies have reported the association of cytotoxic T lymphocyte antigen 4 (CTLA-4) genetic polymorphisms with the susceptibility to head and neck cancer, but the results remain controversial. We therefore will conduct a meta-analysis to investigate the relationship between CTLA-4 genetic polymorphisms and head and neck cancer susceptibility.

Methods: We will systematically search case-control studies for potential eligible studies from Cochrane Library, EMBASE, Google Scholar, PubMed, China Biomedical Database, WanFang database, and China National Knowledge Infrastructure (CNKI). Additionally, we will also examine other sources to avoid missing potential trials. Two authors will independently collect and perform the study selection, data extraction, and study methodological quality. Statistical analyses were utilized using STATA 12.0 and RevMan 5.3, and the odds ratios (ORs) with 95% confidence intervals (95% CI) were used to estimate the strength of the association of CTLA-4 genetic polymorphisms with the susceptibility to head and neck cancer.

Results: This protocol study will assess the relationship between CTLA-4 genetic polymorphisms and head and neck cancer susceptibility.

Conclusion: The findings of this study will provide systematic evidence for future guidance developing and clinical decision making in patients with head and neck cancer.

Ethics And Dissemination: Ethical approval will not be required as this study is a systematic review.

Protocol Registration Number: DOI 10.17605/OSF.IO/BFJTZ (https://osf.io/bfjtz/).
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http://dx.doi.org/10.1097/MD.0000000000023519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738009PMC
December 2020

Resveratrol Ameliorates Systemic Sclerosis via Suppression of Fibrosis and Inflammation Through Activation of SIRT1/mTOR Signaling.

Drug Des Devel Ther 2020 2;14:5337-5348. Epub 2020 Dec 2.

Department of Rheumatology and Immunology, The Second Affiliated Hospital of Hainan Medical University, Haikou, People's Republic of China.

Purpose: Resveratrol (Res) is a natural polyphenolic compound found in several plants and reported as a promising biological molecule with effective anti-fibrosis and anti-inflammatory activities. However, the underlying mechanism of Res on systemic sclerosis (SSc) remains unclear. In the study, we identified the key cellular signaling pathways involved in the Res regulatory process on SSc.

Methods: Res-targeted genes interaction network was constructed using the STITCH database, and the shared Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways involved in both SSc and Res-targeted genes were then identified. The top five enriched KEGG pathways were visualized by GOplot. KEGG pathways associated with Res-targeted genes were established by Pathway Builder Tool 2.0. Quantitative real-time PCR (qRT-PCR) was used to assess the expression of sirtuin 1 (SIRT1), mammalian targeted of rapamycin (mTOR), and cytokines.

Results: Enrichment analysis of Res-targeted genes showed 79 associated pathways, 27 of which were also involved in SSc. Particularly, SIRT1/mTOR signaling was found as one of the crucial regulatory pathways. In vitro results suggested that SIRT1-mediated mTOR degradation ameliorated bleomycin (BLM)-induced fibrosis and inflammation. Res was capable of elevating the SIRT1 level in fibroblasts and partially reversing mTOR-dependent induction of fibrosis and inflammation.

Conclusion: These results indicated that Res is a feasible and effective choice for SSc and therapeutic target of mTOR could be a potential alternative for treatment of SSc.
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http://dx.doi.org/10.2147/DDDT.S281209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719308PMC
December 2020

Phenolic and Volatile Compounds in the Production of Sugarcane Vinegar.

ACS Omega 2020 Dec 17;5(47):30587-30595. Epub 2020 Nov 17.

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/ Guangxi Key Laboratory of Sugarcane Genetic Improvement/ Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530 007, Guangxi, China.

This study aimed to explore the dynamic variations in the phenolic and volatile organic compounds of sugarcane vinegar subjected to different production processes. The determination of phenolic and volatile organic compounds was performed by UPLC-MS and solid phase micro extraction (SPME) coupled with gas chromatography combined with mass spectrometry (GC-MS). The complete fermentation process of sugarcane lasted nine days, and production of vinegar of up to 3.04% (w/v), total acids, and 4.1° alcoholicity was accomplished. Various phenolic compounds of sugarcane juice (non-sterilized) and those of alcoholic and acetic acid fermentation were obtained after nine days of fermentation. These were benzoic acid (2.024, 1.002, and 1.027 mg L), ferulic acid (0.060, 0.205, and 1.124 mg L), quinic acid (0.019, 0.074, and 0.031 mg L), chlorogenic acid (0.349, 1.635, and 1.217 mg L), apigenin (0.002, 0.099, and 0.004 mg L), kaempferol (0.003, 0.336, and 0.003 mg L), caffeic acid (-, 0.005, and 0.005 mg L), luteolin (0.003, 0.323, and 0.005 mg L), and -coumaric acid (0.018, 0.015, and 0.027 mg L). Forty-five volatile organic compounds were also identified. The sugarcane juice can be commercialized as an alternative to wine as it presents characteristics of an alcoholic fermented beverage.
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http://dx.doi.org/10.1021/acsomega.0c04524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714267PMC
December 2020

Immunoreactivity and neutralization study of Chinese Bungarus multicinctus antivenin and lab-prepared anti-bungarotoxin antisera towards purified bungarotoxins and snake venoms.

PLoS Negl Trop Dis 2020 11 30;14(11):e0008873. Epub 2020 Nov 30.

Key Laboratory of Animal Models and Human Disease Mechanisms of The Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan, China.

Bungarus multicinctus is the most venomous snake distributed in China and neighboring countries of Myanmar, Laos, north Vietnam and Thailand. The high mortality rate of B. multicinctus envenomation is attributed to the lethal components of α-, β-, γ- and κ- bungarotoxins contained in the venom. Although anti-B. multicinctus sera were produced in Shanghai, Taiwan and Vietnam, the most widely clinic used product was term as B. multicinctus antivenin and manufactured by Shanghai Serum Bio-technology Co. Ltd. In the present investigation, high purity α-, β- and γ-bungarotoxins were separately isolated from B. multicinctus crude venom. Rabbit anti- α-, β- and γ-bungarotoxin antisera were prepared by common methods, respectively. LD50 values of α-, β- and γ-bungarotoxins were systematically determined via three administration pathways (intraperitoneal, intramuscular and intravenous injections) in Kunming mice. LD50 values of β-bungarotoxin were closely related with injection routines but those of both α- and γ-bungarotoxins were not dependent on the injection routines. Commercial B. multicinctus antivenin showed strong immunoreaction with high molecular weight fractions of the B. multicinctus but weakly recognized low molecular weight fractions like α- and γ-bungarotoxins. Although B. multicinctus antivenin showed immunoreaction with high molecular weight fractions of Bungarus fasciatus, Naja atra, Ophiophagus hannah venoms but the antivenin only demonstrated animal protection efficacy against O. hannah venom. These results indicated that the high molecular weight fractions of the O. hannah played an important role in venom lethality but those of B. fasciatus and N. atra did not have such a role.
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http://dx.doi.org/10.1371/journal.pntd.0008873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728252PMC
November 2020

Comparative transcriptome analysis reveals the regulatory effects of acetylcholine on salt tolerance of Nicotiana benthamiana.

Phytochemistry 2021 Jan 24;181:112582. Epub 2020 Nov 24.

College of Life Sciences, Northwest Agriculture & Forestry University, 712100, Yangling, China. Electronic address:

Salinity is a major cause of crop losses worldwide. Acetylcholine (ACh) can ameliorate the adverse effects of abiotic stresses on plant growth, including salinity stress; however, the underlying molecular mechanisms of this process are unclear. Here, seedlings of Nicotiana benthamiana grown under normal conditions or exposed to 150 mmol L NaCl salinity stress were then treated with a root application of 10 μM ACh. Exogenous ACh application resulted in the downregulation of the activity of the antioxidant enzymes, ascorbate peroxidase, and catalase. ACh-treated plants had lower levels of reactive oxygen species, including the superoxide anion radical and hydrogen peroxide. Transcriptome analysis indicated that ACh treatment under salt stress promoted the differential expression of 658 genes in leaves of N. benthamiana (527 were upregulated and 131 were downregulated). Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that exogenous ACh application was associated with a substantial increase in the transcripts of genes related to cell wall peroxidases, xyloglucan endotransglucosylases or hydrolases, and expansins, indicating that ACh activates cell wall biosynthesis in salt-stressed plants. ACh also enhanced the expression of genes associated with the auxin, gibberellin, brassinosteroid, and salicylic acid signalling pathways, indicating that ACh induces the activation of these pathways under salt stress. Collectively, these findings indicate that ACh-induced salt tolerance in N. benthamiana seedlings is mediated by the inhibition of antioxidant enzymes, activation of cell wall biosynthesis, and hormone signalling pathways. Stress-induced genes involved in osmotic regulation and oxidation resistance were induced by ACh under salt stress. The genes whose transcript levels were elevated by ACh treatment in salt-stressed N. benthamiana could be used as molecular markers of the physiological status of plants under salt stress.
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http://dx.doi.org/10.1016/j.phytochem.2020.112582DOI Listing
January 2021

Classification of the botanical and geographical origins of Chinese honey based on H NMR profile with chemometrics.

Food Res Int 2020 11 21;137:109714. Epub 2020 Sep 21.

Agro-product Safety Research Center, Chinese Academy of Inspection and Quarantine, China.

In this paper, we report a newly developed non-target H NMR detection associated with chemometrics method to classify the botanical and geographical origins of the monofloral Chinese honey. H NMR tests of 218 monofloral honey samples of 8 classes (Acacia, Jujube, Linden, Longan, Orange, Rape, Sunflower, Vitex) collected in 2017-2019 across China were conducted under the optimal sample preparation conditions and NMR acquisition parameters. The whole profiles of NMR spectra instead of individual or partial signals from specific components were processed and extracted, then fed to SIMCA-P to classify the botanical and geographical origins through non-target statistical analysis. For the botanical origins, most of them could be classified clearly according to Principal Component Analysis (PCA) with both R and Q close to 1. Orthogonal Partial Least Squares Discrimination Analysis (OPLS-DA) model could classify the honey floral types successfully with RY and Q greater than 0.85. It is found that the integral bin for data extraction has no obvious influence on the classification. For the geographical origins, the classification at different geographical levels (providence and town) could be successfully distinguished by OPLS-DA model. The promising preliminary results with the geographical classification at 40 km level unambiguously demonstrate the application of this NMR-based multi-species non-targeted method for the honey authenticity. Successful result is obtained on a pilot prediction of the geographical classification. Comparing with the methods based on other techniques, the advantages of this reported one are less sample amount needed, simple preparation, short test time, and non-targeted multi-species detection.
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http://dx.doi.org/10.1016/j.foodres.2020.109714DOI Listing
November 2020

A neural-network based framework of developing cross interaction in alloy embedded-atom method potentials: application to Zr-Nb alloy.

J Phys Condens Matter 2021 Feb;33(8):084004

State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an, 710072, People's Republic of China.

Interaction potentials are critical to molecular dynamics simulations on fundamental mechanisms at atomic scales. Combination of well-developed single-element empirical potentials via cross interaction (CI) is an important and effective way to develop alloy embedded-atom method (EAM) potentials. In this work, based on neural-network (NN) models, firstly we proposed a framework to construct CI potential functions via utilizing single-element potentials. The framework contained four steps: (1) extracting characteristic points from single-element potential functions, (2) constructing CI functions by cubic spline interpolation, (3) evaluating the accuracy of CI functions by referring to first-principle (FP) data, and (4) searching for reasonable CI functions via NN models. Then with this framework, we developed a Zr-Nb alloy CI potential utilizing the MA-III (pure Zr potential developed by Mendelev and Ackland in 2007) and the Fellinger, Park and Wilkins (FPW) (pure Nb potential developed by FPW in 2010) potentials as single-element parts. The calculated results with this Zr-Nb alloy potential showed that: (1) the newly developed CI potential functions could simultaneously present the potential-function features of Zr and Nb; (2) the normalized energy-volume curves of L1 ZrNb, B2 ZrNb and L1 ZrNb calculated by this CI potential reasonably agreed with FP results; (3) the referred MA-III Zr and FPW Nb potentials can satisfactorily reproduce the priority of prismatic slip in Zr and the tension-compression asymmetry of 〈111〉{112} slip in Nb, while other ab initio developed Zr-Nb alloy potentials cannot. Our study indicates that, this NN based framework can take full advantage of single-element potentials, and is very convenient to develop EAM potentials of alloys; moreover, the new-developed Zr-Nb alloy EAM potential can reasonably describe the complicated deformation behaviors in Zr-Nb systems.
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http://dx.doi.org/10.1088/1361-648X/abcb69DOI Listing
February 2021

Clinical Relevance of Plasma Endogenous Tissue-Plasminogen Activator and Aortic Valve Sclerosis: Performance as a Diagnostic Biomarker.

Front Cardiovasc Med 2020 14;7:584998. Epub 2020 Oct 14.

Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Aortic valve sclerosis (AVSc), a common precursor to calcific aortic valve disease, may progress into advanced aortic stenosis with hemodynamic instability. However, plasma biomarkers of such a subclinical condition remain lacking. Since impaired fibrinolysis featuring dysregulated tissue plasminogen activator (t-PA) is involved in several cardiovascular diseases, we investigated whether endogenous t-PA was also associated with AVSc. Plasma t-PA levels were measured in 295 consecutive patients undergoing standard echocardiography and Doppler flow imaging. Multiple logistic regression analyses were used to assess the association between t-PA and AVSc. Receiver operating characteristic curve analysis was performed for determining the diagnostic value of t-PA for AVSc. The performance of adding t-PA to clinical signatures of AVSc was evaluated. Concentration of t-PA was assessed in human sclerotic and non-sclerotic aortic valves by histology and immunohistochemistry analysis. Plasma t-PA was higher in patients with AVSc than in non-AVSc counterparts (median, 2063.10 vs. 1403.17 pg/mL, < 0.01). C-statistics of plasma t-PA for discriminating AVSc was 0.698 (95%CI: 0.639-0.758). The performance of t-PA for identifying AVSc was better among male and non-hypertensive patients [C-statistics (95%CI): 0.712 (0.634-0.790) and 0.805 (0.693-0.916), respectively]. Combination of t-PA and clinical factors improved classification of the patients (category-free NRI: 0.452, < 0.001; IDI: 0.020, = 0.012). The concentration of t-PA was three times higher in sclerotic compared to non-sclerotic aortic valves. Elevated circulating t-PA level confers an increased risk for AVSc. Further prospective studies with larger sample size are needed to examine if t-PA could serve as a diagnostic clinical marker for AVSc.
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http://dx.doi.org/10.3389/fcvm.2020.584998DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591748PMC
October 2020

A benzothiazolium-based fluorescent probe with ideal pK for mitochondrial pH imaging and cancer cell differentiation.

J Mater Chem B 2020 12 30;8(46):10586-10592. Epub 2020 Oct 30.

College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.

A mitochondrial pH sensing fluorescent probe namely 2-(2-(6-hydroxynaphthalen-2-yl)vinyl)-3-(6-(triphenyl-phosphonio)hexyl)benzothiazol-3-ium bromide (HTBT2) was designed and facilely synthesized via the Knoevenagel condensation reaction. HTBT2 displayed a linear fluorescence enhancement at 612 nm in response to pH changes between 8.70 and 7.20. The pK value was determined to be 8.04 ± 0.02, which might be ideal for mitochondrial pH (pH∼8.0) detection. HTBT2 also exhibited a remarkable large Stokes shift of 176 nm, which could diminish the interference of excitation light. The results of live cell imaging studies suggested that HTBT2 showed excellent targeting ability for mitochondria. Importantly, it was successfully applied to visualize mitochondrial pH changes in live cells and differentiate the pH difference between cancer cell lines and normal cell lines. Our results consistently supported that HTBT2 held practical promise for the investigation of physiological processes related to pH changes and clinical potential for cancer cell differentiation.
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http://dx.doi.org/10.1039/d0tb01253jDOI Listing
December 2020

miR-371b-5p promotes cell proliferation, migration and invasion in non-small cell lung cancer via SCAI.

Biosci Rep 2020 11;40(11)

Department of Basic Medicine, Jiangsu College of Nursing, Huai'an, Jiangsu 223005, China.

Objective: Multiple gene targets have been reported for treatment of non-small cell lung cancer (NSCLC), however, the accompanying genetic tolerance was reported increasingly. Therefore, it is important to find new biomarkers or therapeutic targets in treatment of NSCLC.

Methods: The expression levels of miR-371b-5p were detected by qRT-PCR in NSCLC tissues and cell lines. To evaluate the effect of miR-371b-5p on NSCLC progression, we first transfected the miR-371b-5p inhibitor for construction of the miR-371b-5p down-regulated cell model. Then the cell proliferation, migration, invasion and cell apoptosis were detected. In addition, the expression levels of adhesion factors were detected. The target gene of miR-371b-5p was identified by bioinformatics analysis, and rescue experiment was conducted to validate the effect of miR-371b-5p on proliferation, migration and invasion of NSCLC.

Results: Our findings revealed that the miR-371b-5p was overexpressed in NSCLC and could markedly promote the cell proliferation, migration and invasion. Expression levels of both intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were significantly down-regulated when treated by miR-371b-5p inhibitor. Moreover, dual-luciferase reporter assay showed that the miR-371b-5p targeted SCAI in regulation of cell proliferation, migration and invasion, and the expression of miR-371b-5p was negatively associated with SCAI in NSCLC tissues and cell lines. Rescue experiment revealed that the miR-371b-5p could rescue the effect of SCAI on cell proliferation, migration and invasion.

Conclusion: Our results suggest that the miR-371b-5p and SCAI may serve as novel prognostic biomarkers and therapeutic targets for NSCLC.
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http://dx.doi.org/10.1042/BSR20200163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672804PMC
November 2020

A Tandem 0D/2D/2D NbS Quantum Dot/Nb O Nanosheet/g-C N Flake System with Spatial Charge-Transfer Cascades for Boosting Photocatalytic Hydrogen Evolution.

Small 2020 Oct 23;16(42):e2003302. Epub 2020 Sep 23.

School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, 610054, China.

The relatively high recombination rate of charges remains the most critical limiting factor for solar-driven water splitting for hydrogen generation. Herein, a tandem 0D/2D/2D NbS quantum dot/Nb O nanosheet/g-C N flake (NSNOCN) system is designed. Owing to the unique spatial-arrangement and elaborate morphology of 0D NbS , 2D Nb O , and 2D g-C N in the newly designed NSNOCN, plenty of spatial charge-transfer cascades from g-C N to NbS via Nb O are formed to accelerate separation and transfer of charges significantly, thus contributing to a high photocatalytic H generation rate of 13.99 mmol h g (an apparent quantum efficiency of 10.8% at 420 nm), up to 107.6 and 43.7 times by contrast with that of g-C N and Nb O , respectively. This work can provide a new platform in the design of artificial photocatalytic systems with high charge-transfer efficiency.
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http://dx.doi.org/10.1002/smll.202003302DOI Listing
October 2020