Publications by authors named "Bo Li"

5,182 Publications

  • Page 1 of 1

AMPKα2/HNF4A/BORIS/GLUT4 pathway promotes hepatocellular carcinoma cell invasion and metastasis in low glucose microenviroment.

Biochem Pharmacol 2022 Aug 5:115198. Epub 2022 Aug 5.

Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, No. 17, Section 3, South Renmin Road, Chengdu, 610041, Sichuan Province, China. Electronic address:

Increasing evidence has revealed that the invasion and metastasis of HCC are intimately related to the low-glucose microenvironment, but the intrinsic regulatory mechanism remains unclear. It has been well documented that AMPK regulates the transcriptional expression of GLUT4 and its catalytic subunit AMPKα2 can negatively regulate the downstream target molecule HNF4A. Meanwhile, BORIS (Brother of the Regulator of Imprinted Sites) is able to modulate the Warburg effect by regulating the splicing of pyruvate kinase M2 (PKM2), a critical enzyme in glycolysis. Through bioinformatic analysis and a series of overexpression, knockdown, and complementation experiments, we demonstrated that HNF4A can directly act on BORIS and negatively regulate its expression, thereby inhibiting hepatoma cell motility and tumor metastasis, whereas BORIS can directly act on GLUT4 and positively regulate its expression to enhance hepatoma cell motility and tumor metastasis. We also found that HNF4A agonist (Benfluorex) and GLUT4 inhibitor (antiviral drug Ritonavir) can suppress HCC cell proliferation and glucose uptake. Taken together, these results all suggest that activation of the AMPKα2/HNF4A/BORIS/GLUT4 signaling pathway in a low-glucose microenvironment can significantly promote the invasion and metastasis of HCC cells, while HNF4A and GLUT4 may have important potential applications as prognostic or drug target molecules.
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http://dx.doi.org/10.1016/j.bcp.2022.115198DOI Listing
August 2022

Circulating Monocytes Act as a Common Trigger for the Calcification Paradox of Osteoporosis and Carotid Atherosclerosis TGFB1-SP1 and TNFSF10-NFKB1 Axis.

Front Endocrinol (Lausanne) 2022 22;13:944751. Epub 2022 Jul 22.

Department of Orthopedics, Sun Yat-sen Memorial Hospital, Guangzhou, China.

Background: Osteoporosis often occurs with carotid atherosclerosis and causes contradictory calcification across tissue in the same patient, which is called the "calcification paradox". Circulating monocytes may be responsible for this unbalanced ectopic calcification. Here, we aimed to show how CD14 monocytes contribute to the pathophysiology of coexisting postmenopausal osteoporosis and carotid atherosclerosis.

Methods: We comprehensively analyzed osteoporosis data from the mRNA array dataset GSE56814 and the scRNA-seq dataset GSM4423510. Carotid atherosclerosis data were obtained from the GSE23746 mRNA dataset and GSM4705591 scRNA-seq dataset. First, osteoblast and vascular SMC lineages were annotated based on their functional expression using gene set enrichment analysis and scoring. Next, analysis was applied to draw their differentiated trajectory and identify the key gene expression changes in crossroads. Then, ligand-receptor interactions between CD14 monocytes and osteoblast and vascular smooth muscle cell (SMC) lineages were annotated with . Finally, we selected calcification paradox-related expression in circulating monocytes with analysis.

Results: First, we found a large proportion of delayed premature osteoblasts in osteoporosis and osteogenic SMCs in atherosclerosis. Second, CD14 monocytes interacted with the intermediate cells of the premature osteoblast and osteogenic SMC lineage by delivering TGFB1 and TNFSF10. This interaction served as a trigger activating the transcription factors (TF) SP1 and NFKB1 to upregulate the inflammatory response and cell senescence and led to a retarded premature state in the osteoblast lineage and osteogenic transition in the SMC lineage. Then, 76.49% of common monocyte markers were upregulated in the circulating monocytes between the two diseases, which were related to chemotaxis and inflammatory responses. Finally, we identified 7 calcification paradox-related genes on circulating monocytes, which were upregulated in aging cells and downregulated in DNA repair cells, indicating that the aging monocytes contributed to the development of the two diseases.

Conclusions: Our work provides a perspective for understanding the triggering roles of CD14 monocytes in the development of the calcification paradox in osteoporosis- and atherosclerosis-related cells based on combined scRNA and mRNA data. This study provided us with an elucidation of the mechanisms underlying the calcification paradox and could help in developing preventive and therapeutic strategies.
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http://dx.doi.org/10.3389/fendo.2022.944751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354531PMC
July 2022

Recent Advance in Tumor Microenvironment-Based Stimuli-Responsive Nanoscale Drug Delivery and Imaging Platform.

Front Pharmacol 2022 22;13:929854. Epub 2022 Jul 22.

Department of Radiology, The Second Hospital of Jilin University, Changchun, China.

The tumor microenvironment (TME) plays an important role in the development, progression, and metastasis of cancer, and the extremely crucial feature is hypoxic and acidic. Cancer-associated fibroblasts (CAFs), extracellular matrix (ECM), mesenchymal cells, blood vessels, and interstitial fluid are widely recognized as fundamentally crucial hallmarks for TME. As nanotechnology briskly boomed, the nanoscale drug delivery and imaging platform (NDDIP) emerged and has attracted intensive attention. Based on main characteristics of TME, NDDIP can be classified into pH-sensitive delivery and imaging platforms, enzyme-sensitive delivery and imaging platforms, thermo-sensitive delivery and imaging platforms, redox-sensitive delivery and imaging platforms, and light-sensitive delivery and imaging platforms. Furthermore, imageology is one of the significant procedures for disease detection, image-guided drug delivery, and efficacy assessment, including magnetic resonance imaging (MRI), computed tomography (CT), ultrasound (US), and fluorescence imaging. Therefore, the stimuli-responsive NDDIP will be a versatile and practicable tumor disease diagnostic procedure and efficacy evaluation tool. In this review article, we mainly introduce the characteristics of TME and summarize the progress of multitudinous NDDIP as well as their applications.
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http://dx.doi.org/10.3389/fphar.2022.929854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354407PMC
July 2022

Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention.

Front Bioeng Biotechnol 2022 22;10:944435. Epub 2022 Jul 22.

Department of Vascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Endovascular interventions, such as balloon dilation and stent implantation, are currently recommended as the primary treatment for patients with peripheral artery disease (PAD), greatly improving patient prognosis. However, the consequent lumen restenosis that occurs after endovascular interventions has become an important clinical problem. Inflammation has been proven to be crucial to postoperative restenosis. In previous studies we have identified that Netrin-1-modified adipose-derived stem cells (N-ADSCs) transplantation is an effective anti-inflammatory strategy to repair vascular damage. Nevertheless, it remained to be explored how one could constantly deliver N-ADSCs onto damaged arteries. Therefore, we developed an adhesive double network (DN) hydrogel wrap loaded with N-ADSCs for sustained perivascular delivery. Inspired by the adhesion mechanism of mussels, we developed an adhesive and tough polyacrylamide/calcium-alginate/reduced graphene oxide/polydopamine (PAM/CA/rGO/PDA) hydrogel. Dopamine was attached to graphene sheets and limitedly oxidized to generate free catechol groups. The hydrogel could wrap damaged arteries and induce anti-inflammatory effects through N-ADSCs. experiments demonstrated that N-ADSCs significantly promoted the M2 polarization of macrophages to anti-inflammatory phenotypes and reduced the expression of inflammatory factors. experiments in a rat carotid artery guidewire injury model showed that the adhesive hydrogel wrap loaded with N-ADSCs could significantly reduce arterial inflammation, inhibit intimal hyperplasia and improve re-endothelialization. Altogether, this newly developed N-ADSCs-loaded hydrogel wrap provides an effective slow-releasing system, which may be a promising way to prevent and treat restenosis after endovascular interventions.
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http://dx.doi.org/10.3389/fbioe.2022.944435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355160PMC
July 2022

Combinatorial transcriptional regulation of HEB/ZEB1/ASCL1 and MYBL2 on Ras/ErbB signaling.

Biochem Biophys Res Commun 2022 Jul 14;622:170-176. Epub 2022 Jul 14.

Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China; Liaoning Provincial Engineering Laboratory of Tumor Immunity and Molecular Diagnosis and Treatment Techniques, Jinzhou Medical University, Jinzhou, 121001, China. Electronic address:

Gene expression is tightly regulated by transcription factors (TFs) which play an important role in development and tumorigenesis. Abnormal transcriptional regulation leads to oncogene activation or tumor suppressor inhibition, thus promoting the occurrence and progression of tumors. MYBL2 (alias B-Myb), a ubiquitously expressed transcription factor of the MYB family, is a nuclear protein involved in cell cycle progression and overexpressed and associated with poor patient outcomes in numerous cancer entities. However, the further effectors of the MYBL2 downstream transcriptional network mediating its cancer-promoting properties remain not well elaborated. Here, we systemic investigated the global MYBL2 targets base on ChIP-seq data from melanoma, breast cancer, lung carcinoma, and liver cancer. Functional enrichment and further validation of MYBL2 downstream binding targets on melanoma cells demonstrated that genes in the Ras and ErbB signaling pathways were regulated by MYBL2. Moreover, when integrating breast cancer, lung carcinoma and liver cancer data, we identified HEB, ZEB1 and ASCL1 colocalized on Ras/ErbB signaling gene locus with MYBL2, indicating the regulatory complex on activating oncogenic expression. Taken together, this study provides a reference for a better understanding of the MYBL2 regulatory mechanism in tumorigenesis.
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http://dx.doi.org/10.1016/j.bbrc.2022.07.046DOI Listing
July 2022

Multiparametric MR radiomics in brain glioma: models comparation to predict biomarker status.

BMC Med Imaging 2022 Aug 5;22(1):137. Epub 2022 Aug 5.

Department of Imaging Diagnosis, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010000, China.

Background: Genotype status of glioma have important significance to clinical treatment and prognosis. At present, there are few studies on the prediction of multiple genotype status in glioma by method of multi-sequence radiomics. The purpose of the study is to compare the performance of clinical features (age, sex, WHO grade, MRI morphological features etc.), radiomics features from multi MR sequence (T2WI, T1WI, DWI, ADC, CE-MRI (contrast enhancement)), and a combined multiple features model in predicting biomarker status (IDH, MGMT, TERT, 1p/19q of glioma.

Methods: In this retrospective analysis, 81 glioma patients confirmed by histology were enrolled in this study. Five MRI sequences were used for radiomic feature extraction. Finally, 107 features were extracted from each sequence on Pyradiomics software, separately. These included 18 first-order metrics, such as the mean, standard deviation, skewness, and kurtosis etc., 14 shape features and second-order metrics including 24 grey level run length matrix (GLCM), 16 grey level run length matrix (GLRLM), 16 grey level size zone matrix (GLSZM), 5 neighboring gray tone difference matrix (NGTDM), and 14 grey level dependence matrix (GLDM). Then, Univariate analysis and LASSO (Least absolute shrinkage and selection operator regression model were used to data dimension reduction, feature selection, and radiomics signature building. Significant features (p < 0.05 by multivariate logistic regression were retained to establish clinical model, T1WI model, T2WI model, T1 + C (T1WI contrast enhancement model, DWI model and ADC model, multi sequence model. Clinical features were combined with multi sequence model to establish a combined model. The predictive performance was validated by receiver operating characteristic curve (ROC analysis and decision curve analysis (DCA).

Results: The combined model showed the better performance in some groups of genotype status among some models (IDH AUC = 0.93, MGMT AUC = 0.88, TERT AUC = 0.76). Multi sequence model performed better than single sequence model in IDH, MGMT, TERT. There was no significant difference among the models in predicting 1p/19q status. Decision curve analysis showed combined model has higher clinical benefit than multi sequence model.

Conclusion: Multi sequence model is an effective method to identify the genotype status of cerebral glioma. Combined with clinical models can better distinguish genotype status of glioma.

Key Points: The combined model showed the higher performance compare with other models in predicting genotype status of IDH, MGMT, TERT. Multi sequence model showed a better predictive model than that of a single sequence model. Compared with other models, the combined model and multi sequence model show no advantage in prediction of 1p/19q status.
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http://dx.doi.org/10.1186/s12880-022-00865-8DOI Listing
August 2022

Identification of cytochrome c oxidase subunit 4 isoform 1 as a positive regulator of influenza virus replication.

Front Microbiol 2022 19;13:862205. Epub 2022 Jul 19.

Center for Bioactive Natural Molecules and Innovative Drugs Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, China.

Human infection with highly pathogenic H5N1 influenza virus causes severe respiratory diseases. Currently, the drugs against H5N1 are limited to virus-targeted inhibitors. However, drug resistance caused by these inhibitors is becoming a serious threat to global public health. An alternative strategy to reduce the resistance risk is to develop antiviral drugs targeting host cell proteins. In this study, we demonstrated that cytochrome c oxidase subunit 4 isoform 1 (COX41) of host cell plays an important role in H5N1 infection. Overexpression of COX41 promoted viral replication, which was inhibited by silencing or knockout the expression of COX41 in the host cell. The ribonucleoproteins (RNPs) of H5N1 were retained in the cell nucleus after knockout cellular COX41. Strikingly, inhibition of cellular COX41 by lycorine, a small-molecule compound isolated from Amaryllidaceae plants, reduced the levels of COX41-induced ROS and phosphorylation of extracellular signal-regulated kinase (ERK) in cells, thus resulting in the blockage of nuclear export of vRNP and inhibition of viral replication. In H5N1-infected mice that were treated with lycorine, we observed a reduction of viral titers and inhibition of pathological changes in the lung and trachea tissues. Importantly, no resistant virus was generated after culturing the virus with the continuous treatment of lycorine. Collectively, these findings suggest that COX41 is a positive regulator of H5N1 replication and might serve as an alternative target for anti-influenza drug development.
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http://dx.doi.org/10.3389/fmicb.2022.862205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343726PMC
July 2022

Long noncoding RNA CERS6-AS1 modulates glucose metabolism and tumor progression in hepatocellular carcinoma by promoting the MDM2/p53 signaling pathway.

Cell Death Discov 2022 Aug 4;8(1):348. Epub 2022 Aug 4.

Department of Liver Surgery and Liver Transplantation Center, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

Hepatocellular carcinoma (HCC) is one of the most serious malignant cancers and has a high fatality rate. However, clinical strategies for the effective treatment of HCC remain lacking. Long non-coding RNAs (lncRNAs) with aberrant expression have been closely correlated with the occurrence and development of HCC. Here we investigated the underlying mechanism of the lncRNA CERS6-AS1 in HCC progression. The expression and prognosis of CERS6-AS1 in HCC patients was explored using The Cancer Genome Atlas. PCR analysis was utilized to measure the expression of CERS6-AS1 in tissues and cell lines. Transwell, wound healing, proliferation and glycolysis assays were conducted to evaluate the function of CERS6-AS1 on HCC cell functions. Bioinformation methods and luciferase assays were used to screen and verify potential target miRNAs and genes. A subcutaneous tumorigenesis model was constructed in nude mice to assess the effect of CERS6-AS1 on tumorigenesis in vivo. CERS6-AS1 was highly expressed in HCC tissues and cell lines. Upregulated CERS6-AS1 expression was remarkably correlated with poor prognosis of HCC patients. High CERS6-AS1 expression facilitated cell growth, invasion and glycolysis of HCC cells. Bioinformatics analyses combining with PCR analysis identified miR-30b-3p as the potential target of CERS6-AS1, and MDM2 mRNA was verified as the target of miR-30b-3p. The expression of miR-30b-3p was negatively correlated with CERS6-AS1, whereas MDM2 was positively associated with CERS6-AS1. Mechanistic studies showed that CERS6-AS1 may sponge miR-30b-3p to elevate MDM2, thus promoting the MDM2-mediated ubiquitin-dependent degradation of the p53 tumor suppressor. MDM2 overexpression or miR-30b-3p inhibitors blocked the inhibitory effect of CERS6-AS1 knockdown on proliferation, migration and glycolysis. CERS6-AS1 depletion reduced tumor formation in the in vivo mouse model. The CERS6-AS1/miR-30b-3p/MDM2/p53 signaling axis may play key roles in regulating HCC progression. CERS6-AS1 may exert as a novel biomarker or therapeutic target for HCC.
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http://dx.doi.org/10.1038/s41420-022-01150-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352870PMC
August 2022

An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motif.

Sci Immunol 2022 Aug 4:eabp9962. Epub 2022 Aug 4.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148, USA.

The rapid evolution of SARS-CoV-2 viruses, such as the Omicron variants which are highly transmissible and immune evasive, underscores the need to develop therapeutic antibodies with broad neutralizing activities. Here, we used the LIBRA-seq technology, which identified SARS-CoV-2 specific B cells via DNA-barcoding and subsequently single cell sequenced BCRs, to identify an antibody, SW186, which could neutralize major SARS-CoV-2 variants of concern, including Beta, Delta, and Omicron, as well as SARS-CoV-1. The cryo-EM structure of SW186 bound to the receptor-binding domain (RBD) of the viral spike protein showed that SW186 interacted with an epitope of the RBD that is not at the interface of its binding to the ACE2 receptor but highly conserved among SARS coronaviruses. This epitope encompasses a glycosylation site (N343) of the viral spike protein. Administration of SW186 in mice after they were infected with SARS-CoV-2 Alpha, Beta, or Delta variants reduced the viral loads in the lung. These results demonstrated that SW186 neutralizes diverse SARS coronaviruses by binding to a conserved RBD epitope, which could serve as a target for further antibody development.
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http://dx.doi.org/10.1126/sciimmunol.abp9962DOI Listing
August 2022

The MVBML approach: a management for buccal cancer with wider indications and higher satisfaction.

Oral Dis 2022 Aug 4. Epub 2022 Aug 4.

Department of Oral and Maxillofacial-Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, 100050, Beijing, P.R. China.

Objective: The purpose of this study is to explore the effects of MVBML approach on postoperative facial scar, nerves injury and prognosis of patients with BSCC.

Patients And Methods: This is a single-center, prospective cohort study that enrolled 80 patients with BSCC from June 2015 to December 2020. According to the different surgical approaches, the patients were divided into two groups: the lower lip median (LLM) approach group and the modified vermilion border-marionette line (MVBML) approach group.

Results: The results showed that the appearance (p=0.003), scar consciousness (p<0.001) and satisfaction with appearance (p=0.001) of patients in the MVBML group were significantly better than those in the LLM group, and the difference was more obvious in elderly group. Statistical analysis of postoperative nerves injury showed that the MVBML group had a lower risk of facial and mental nerves injury than the LLM group, and there was a significant statistical difference in mental nerve injury between the two groups (p<0.001). Through Kaplan-Meier survival analysis, we found no significant difference in disease-specific survival (p=0.47) or disease-free survival (p=0.70) between the LLM approach group and the MVBML approach group.

Conclusions: The MVBML surgical approach is worthy of advancement for the surgical treatment of BSCC.
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http://dx.doi.org/10.1111/odi.14340DOI Listing
August 2022

Silicon micron cages derived from a halloysite nanotube precursor and aluminum sacrificial template in molten AlCl as an anode for lithium-ion batteries.

RSC Adv 2022 Jul 21;12(32):20850-20856. Epub 2022 Jul 21.

Beijing National Laboratory for Molecular Sciences (BNLMS), The State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University Beijing 100871 China.

Porous nanostructures have been proposed a promising strategy to improve the electrochemical performance of Si materials as anodes of lithium-ion batteries (LIBs). However, expensive raw materials and the tedious preparation processes hinder their widespread adoption. In this work, silicon micron cages (SMCs) have been synthesized in molten AlCl through using spherical aluminum particles as a sacrificial template, and the earth-abundant and low-cost natural halloysite clay as a precursor. The aluminum spheres (1-3 μm) not only act as a sacrificial template but also facilitate the formation of silicon branches, which connect together to form SMCs. As anodes for LIBs, the SMC electrode exhibits a high reversible capacity of 1977.5 mA h g after 50 cycles at a current density of 0.2 A g, and 1035.1 mA h g after 300 cycles at a current density of 1.0 A g. The improved electrochemical performance of SMCs could be ascribed to the micron cage structure, providing abundant buffering space and mesopores for Si expansion. This promising method is expected to offer a pathway towards the scalable application of Si-based anode materials in the next-generation LIB technology.
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http://dx.doi.org/10.1039/d2ra01394kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301631PMC
July 2022

Scanning a multi-channel spin-exchange relaxation-free atomic magnetometer with high spatial and time resolution.

Opt Lett 2022 Aug;47(15):3908-3911

The emerging multi-channel spin-exchange relaxation-free (SERF) atomic magnetometer is a promising candidate for non-intrusive biomagnetism imaging. In this study, we propose a scanning 9-channel SERF magnetometer based on an acousto-optic modulator (AOM). Using the diffraction light of the AOM as the probe laser (with a low laser power of 1.7 mW), 9 channels were rapidly scanned by altering the diffraction angle. The scanning imaging scheme provides a new, to the best of our knowledge, approach for multi-channel magnetic field measurement and realizes a single-channel sensitivity of about 3 fT/Hz, a spatial resolution of 0.6 mm, and a time resolution of about 2.7 ms, which is well suited for real-time extremely weak magnetic field imaging.
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http://dx.doi.org/10.1364/OL.465832DOI Listing
August 2022

Comparing the Conventional and Balloon-Guided Catheter-Assisted SWIM Technology for the Treatment of Acute Ischemic Stroke.

Front Neurol 2022 13;13:866673. Epub 2022 Jul 13.

Department of Interventional Radiology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objective: Acute ischemic stroke is common in elder patients. This study investigates whether using the balloon-guided catheter (BGC) would improve the effect of stent thrombectomy (Solitaire FR With Intracranial Support Catheter for Mechanical Thrombectomy, SWIM) for patients with acute ischemic stroke due to large vessel occlusion (AIS-LVO).

Method: The data of 209 patients with AIS-LVO underwent SWIM were collected retrospectively from January 2017 to June 2021. These patients were divided into two groups based on whether they used of BGC or not. The propensity score matching (PSM) analysis was used to compare the differences in the first pass effect (FPE), successful recanalization, embolus escape rate, symptomatic intracranial hemorrhage (sICH), 90-day clinical favorable outcome, 90-day all-cause mortality, and complications in the patients treated with SWIM combined with balloon-guided catheter or conventional catheter.

Results: Among the 209 patients, 44 patients were treated with BGC and 165 patients were not. After matching, a total of 111 patients were included. The results showed that there was no statistical difference in FPE (35.1% in non-BGC group compared to 24.3% in BGC group, matched RR, 0.59; 95% CI, 0.24-1.44), successful recanalization (89.2 vs. 91.9%, matched RR, 1.37; 95%CI, 0.34-5.51), embolus escape (6.8 vs. 8.1%, matched RR, 1.22; 95%CI, 0.28-5.40), sICH (8.1 vs. 13.5%; matched RR, 1.77; 95%CI 0.50-6.24), 90-day clinical favorable outcome (48.7 vs. 54.1%, matched RR, 1.11; 95%CI 0.51-2.46), 90-day all-cause mortality (17.6 vs. 21.6%, matched RR, 1.29; 95%CI 0.48-3.47), and the incidence of complications (6.8 vs. 5.4%, matched RR, 0.79 95%CI 0.15-4.27). These results indicate that using SWIM as the first-line treatment for patients with AIS-LVO, there is no statistical significance in FPE, final successful recanalization, distal emboli, sICH, procedural time, 90-day favorable outcome, 90-day mortality, and complications with or without BGC.

Conclusion: Balloon-guided catheter does not affect the result of using SWIM as the first-line treatment for patients with AIS-LVO. Our results will guide daily practice, with the adoption of the use of a guided catheter without a balloon.
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http://dx.doi.org/10.3389/fneur.2022.866673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326023PMC
July 2022

Physical Fitness, Exercise Behaviors, and Sense of Self-Efficacy Among College Students: A Descriptive Correlational Study.

Front Psychol 2022 15;13:932014. Epub 2022 Jul 15.

Physical Education College, Yangzhou University, Yangzhou, China.

Background: Self-efficacy is an important component of the mental well-being of college students. This study aimed to evaluate the development and the correlation between physical fitness (PF), exercise behavior, and self-efficacy in college students. To examine whether PF in individual college students can predict self-efficacy, and whether exercise behavior mediates this relationship.

Methods: This was an observational study of 1923 randomly selected college students (50.5% girl). Measures included the Physical Activity Rating Scale, the Self-Efficacy Scale, and PF testing.

Results: Self-efficacy was weakly correlated to both PF ( = 0.240) and exercise behavior ( = 0.248). In regression analysis, PF explained 24.7% of the variance in self-efficacy, increasing to 29.4% when exercise behavior was considered. Therefore, the predictive effect of PF on self-efficacy is partially realized through healthy exercise behavior.

Conclusion: Physical fitness can predict self-efficacy among college students, with exercise behavior being an important mediation of this relationship. Strategies to improve positive exercise behaviors and PF could improve students' self-efficacy and overall mental health.
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http://dx.doi.org/10.3389/fpsyg.2022.932014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335150PMC
July 2022

Preparation and Performance Investigation of Optimized Cement-Based Sealing Materials Based on the Response Surface Methodology.

ACS Omega 2022 Jul 12;7(29):25380-25393. Epub 2022 Jul 12.

School of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo, Henan 454003, China.

A new cement-based sealing material, which used Portland cement (PC) as a raw material and supplemented several gel components, such as accelerant, alkali activator, suspension agent, expansion agent, reinforcing agent, was prepared in this work. The effects of these components on the fluidity, setting time, and expansion rate of these sealing materials were investigated by an orthogonal test. The results show that the water-cement ratio and the reinforcing agent content, the accelerant content and the water-cement ratio, and the expansion agent content and the accelerant content are the most important influencing factors on fluidity, setting time, and expansion rate, respectively. In addition, the regression models and response surfaces of the factors were established using a multiple linear regression method. By this means, the influences of the two main factors on each performance of this sealing material were accurately and intuitively reflected for obtaining the optimal value in the optimization area. The results indicate that the sealing materials possess the best performances when the water-cement ratio is 1.1, the accelerant content is 50%, the expansion agent content is 0.1%, and the reinforcing agent content is 3%, which is corresponding to a fluidity of 360-380 mm, an initial (final) setting time of 60 (80)-80 (100) min, and an expansion rate of 2-12%. Furthermore, the microstructures of the optimized sealing material also reveal that the main hydration products of PC are transformed from layered Ca(OH) crystals into fine needle-like AFt crystals and C-S-H gels by the promotion effect of the optimizing ratio, thus leading to a more compact structure of optimized cement-based sealing materials.
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http://dx.doi.org/10.1021/acsomega.2c02334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330101PMC
July 2022

Root-Associated Microbiota Response to Ecological Factors: Role of Soil Acidity in Enhancing Citrus Tolerance to Huanglongbing.

Front Plant Sci 2022 15;13:937414. Epub 2022 Jul 15.

State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding, China.

The citrus orchards in southern China are widely threatened by low soil pH and Huanglongbing (HLB) prevalence. Notably, the lime application has been used to optimize soil pH, which is propitious to maintain root health and enhance HLB tolerance of citrus; however, little is known about the interactive effects of soil acidity on the soil properties and root-associated (rhizoplane and endosphere) microbial community of HLB-infected citrus orchard. In this study, the differences in microbial community structures and functions between the acidified and amended soils in the Gannan citrus orchard were investigated, which may represent the response of the host-associated microbiome in diseased roots and rhizoplane to dynamic soil acidity. Our findings demonstrated that the severity of soil acidification and aluminum toxicity was mitigated after soil improvement, accompanied by the increase in root activity and the decrease of HLB pathogen concentration in citrus roots. Additionally, the Illumina sequencing-based community analysis showed that the application of soil amendment enriched functional categories involved in host-microbe interactions and nitrogen and sulfur metabolisms in the HLB-infected citrus rhizoplane; and it also strongly altered root endophytic microbial community diversity and structure, which represented by the enrichment of beneficial microorganisms in diseased roots. These changes in rhizoplane-enriched functional properties and microbial composition may subsequently benefit the plant's health and tolerance to HLB disease. Overall, this study advances our understanding of the important role of root-associated microbiota changes and ecological factors, such as soil acidity, in delaying and alleviating HLB disease.
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http://dx.doi.org/10.3389/fpls.2022.937414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335078PMC
July 2022

The Effects of Bone Cement Volume in Percutaneous Vertebroplasty for Thoracolumbar Junction Vertebral Compression Fractures: A Clinical Comparative Study.

Mediators Inflamm 2022 22;2022:4230065. Epub 2022 Jul 22.

Department of Clinic of Spine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.

We compared the outcomes of patients treated with different volumes of polymethyl methacrylate bone cement during percutaneous vertebroplasty (PVP) for thoracolumbar vertebral compression fractures. We performed a comparative, retrospective study of 316 patients who underwent PVP for a single-level thoracolumbar vertebral compression fracture. Patients were divided into two groups: group A (≤5 mL; = 146) and group B (>5 mL; = 170). The visual analogue scale (VAS) for pain and the Roland-Morris Disability Questionnaire (RDQ) scores were compared between the two groups at 1 week and at 1, 6, 12, and 24 months after PVP. The incidence of cement leakage into the intervertebral discs was evaluated by a postoperative lateral radiograph assessment. Patients were evaluated for new fractures 1 and 2 years after PVP or when new fractures were suspected. Among the 316 patients enrolled, 245 completed the clinical research. No difference between groups A and B in terms of the VAS, RDQ, and rate of complications at all time points after surgery was observed. The presence of intervertebral disc leakage was a relative risk (RR) for subsequent total vertebral fracture (RR, 6.42; 95% confidence interval (CI), 2.72-14.19; < 0.0001) and adjacent vertebral fracture (RR, 8.03; 95% CI, 2.74-23.54; = 0.0001). A high volume of bone cement may increase the rate of subsequent total and adjacent vertebral fractures. However, the occurrence of intervertebral disc leakage is the principal risk factor for these negative outcomes of PVP.
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http://dx.doi.org/10.1155/2022/4230065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337957PMC
August 2022

Preoperative Neutrophil-Lymphocyte Ratio for predicting surgery-related acute kidney injury in non-cardiac surgery patients under general anaesthesia: A retrospective cohort study.

PLoS One 2022 29;17(7):e0270066. Epub 2022 Jul 29.

Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha, China.

Background: This study was conducted to investigate the relationship between the Neutrophil-Lymphocyte Ratio (NLR) and the incidence of surgery-related acute kidney injury (AKI) in non-cardiac surgery patients under general anaesthesia.

Methods: In this retrospective study, 5057 patients from Third Xiangya hospital from January 2012 to December 2016 and 1686 patients from Second Xiangya hospital from January 2016 to December 2016 for non-cardiac surgery under general anesthesia were included. According to receiver operating characteristic (ROC) curve constructed by NLR for postoperative AKI, the cut-off point was obtained as the basis for grouping low or high NLR. The baseline characteristics of two sets were compared with each other. A multi-factor model was constructed by Least absolute shrinkage and selection operator (LASSO) method with the training set, and verified by outside validation set.

Results: 243 patients (3.604%) developed postoperative AKI. The ROC curve showed that the AUC of the NLR for predicting postoperative AKI in non-cardiac surgery was 0.743 (95% CI, 0.717-0.769), and the cut-off value was 3.555 (sensitivity, 86.4%; specificity 51.9%). There was no significant difference in the baseline characteristics of training set and validation set. The AUC in the training set was 0.817 (95% CI, 0.784-0.850), and the AUC in the validation set was 0.804 (95% CI, 0.749-0.858), the AUC deviation was 0.012 (P > 0.05) from validation set, and the likelihood ratio test showed P < 0.05.

Conclusion: This study showed that preoperative high NLR (NLR≥3.555) was an independent risk factor associated with postoperative AKI (OR, 2.410; 95% CI, 1.371-4.335) in patients for non-cardiac surgery under general anesthesia.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270066PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337669PMC
August 2022

Predictive Value of Glycosylated Hemoglobin for Post-operative Acute Kidney Injury in Non-cardiac Surgery Patients.

Front Med (Lausanne) 2022 11;9:886210. Epub 2022 Jul 11.

Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha, China.

Objective: Recent studies have indicated that patients (both with and without diabetes) with elevated hemoglobin A1c (HbA1c) have a higher rate of acute kidney injury (AKI) following cardiac surgery. However, whether HbA1c could help to predict post-operative AKI in patients after non-cardiac surgery is less clear. This study aims to explore the predictive value of pre-operative HbA1c for post-operative AKI in non-cardiac surgery.

Methods: We reviewed the medical records of patients (≥ 18 years old) who underwent non-cardiac surgery between 2011 and 2020. Patient-related variables, including demographic and laboratory and procedure-related information, were collected, and univariable and multivariable logistic regression analyses were performed to determine the association of HbA1c with AKI. The area under the receiver operating curve (AUC), net reclassification improvement index (NRI), and integrated discriminant improvement index (IDI) were used to evaluate the predictive ability of the model, and decision curve analysis was used to evaluate the clinical utility of the HbA1c-added predictive model.

Results: A total of 3.3% of patients (94 of 2,785) developed AKI within 1 week after surgery. Pre-operative HbA1c was an independent predictor of AKI after adjustment for some clinical variables (OR comparing top to bottom quintiles 5.02, 95% CI, 1.90 to 13.24, < 0.001 for trend; OR per percentage point increment in HbA1c 1.20, 95% CI, 1.07 to 1.33). Compared to the model with only clinical variables, the incorporation of HbA1c increased the model fit, modestly improved the discrimination (change in area under the curve from 0.7387 to 0.7543) and reclassification (continuous net reclassification improvement 0.2767, 95% CI, 0.0715 to 0.4818, improved integrated discrimination 0.0048, 95% CI, -5e-04 to 0.0101) of AKI and non-AKI cases, NRI for non-AKI improvement 0.3222, 95% CI, 0.2864 to 0.3580 and achieved a higher net benefit in decision curve analysis.

Conclusion: Elevated pre-operative HbA1c was independently associated with post-operative AKI risk and provided predictive value in patients after non-cardiac surgery. HbA1c improved the predictive power of a logistic regression model based on traditional clinical risk factors for AKI. Further prospective studies are needed to demonstrate the results and clinical application.
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http://dx.doi.org/10.3389/fmed.2022.886210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309303PMC
July 2022

Polysaccharide, the Active Component of , Ameliorates Metabolic Hypertension in Rats Regulating Intestinal Flora-SCFAs-Vascular Axis.

Front Pharmacol 2022 11;13:935714. Epub 2022 Jul 11.

Zhejiang University of Technology, Hangzhou, China.

Metabolic hypertension (MH) is the most common type of hypertension worldwide because of unhealthy lifestyles, such as excessive alcohol intake and high-sugar/high-fat diets (ACHSFDs), adopted by humans. Poor diets lead to a decrease in the synthesis of short-chain fatty acids (SCFAs), which are produced by intestinal flora and transferred by G protein-coupled receptors (GPCRs), resulting in impaired gastrointestinal function, disrupted metabolic processes, increased blood pressure (BP), and ultimately, MH. It is not clear whether polysaccharide (DOPS) can mediate its effects by triggering the SCFAs-GPCR43/41 pathway. In this study, DOPS, with a content of 54.45 ± 4.23% and composition of mannose, glucose, and galacturonic acid at mass percentages of 61.28, 31.87, and 2.53%, was isolated from . It was observed that DOPS, given to rats by intragastric administration after dissolution, could lower the BP and improve the abnormal lipid metabolic processes in ACHSFD-induced MH rats. Moreover, DOPS was found to increase the production, transportation, and utilization of SCFAs, while improving the intestinal flora and strengthening the intestinal barrier, as well as increasing the intestinal levels of SCFAs and the expression of GPCR43/41. Furthermore, DOPS improved vascular endothelial function by increasing the expression of GPCR41 and endothelial nitric oxide synthase in the aorta and the nitric oxide level in the serum. However, these effects were all reversed by antibiotic use. These findings indicate that DOPS is the active component of , and it can reverse MH in rats by activating the intestinal SCFAs-GPCR43/41 pathway.
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http://dx.doi.org/10.3389/fphar.2022.935714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310040PMC
July 2022

The Exosome Journey: From Biogenesis to Regulation and Function in Cancers.

J Oncol 2022 18;2022:9356807. Epub 2022 Jul 18.

Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China.

Exosomes are a type of small endosomal-derived vesicles ranging from 30 to 150 nm, which can serve as functional mediators in cell-to-cell communication and various physiological and pathological processes. In recent years, exosomes have emerged as crucial mediators of intracellular communication among tumor cells, immune cells, and stromal cells, which can shuttle bioactive molecules, such as proteins, lipids, RNA, and DNA. Exosomes exhibit the high bioavailability, biological stability, targeting specificity, low toxicity, and immune characteristics, suggesting their potentials in the diagnosis and treatment of cancers. They can be applied as an effective tool in the diagnostics, therapeutics, and drug delivery in cancers. This review summarizes the regulation and functions of exosomes in various cancers to augment our understanding of exosomes, which paves the way for parallel advancements in the therapeutic approach of cancers. In this review, we also discuss the challenges and prospects for clinical application of exosome-based diagnostics and therapeutics for cancers.
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http://dx.doi.org/10.1155/2022/9356807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313905PMC
July 2022

Structure and Function of a Dehydrating Condensation Domain in Nonribosomal Peptide Biosynthesis.

J Am Chem Soc 2022 Jul 27. Epub 2022 Jul 27.

Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

Dehydroamino acids are important structural motifs and biosynthetic intermediates for natural products. Many bioactive natural products of nonribosomal origin contain dehydroamino acids; however, the biosynthesis of dehydroamino acids in most nonribosomal peptides is not well understood. Here, we provide biochemical and bioinformatic evidence in support of the role of a unique class of condensation domains in dehydration (C). We also obtain the crystal structure of a C domain, which is part of the nonribosomal peptide synthetase AmbE in the biosynthesis of the antibiotic methoxyvinylglycine. Biochemical analysis reveals that AmbE-C modifies a peptide substrate that is attached to the donor carrier protein. Mutational studies of AmbE-C identify several key residues for activity, including four residues that are mostly conserved in the C subfamily. Alanine mutation of these conserved residues either significantly increases or decreases AmbE activity. AmbE exhibits a dimeric conformation, which is uncommon and could enable transfer of an intermediate between different protomers. Our discovery highlights a central dehydrating function for C domains that unifies dehydroamino acid biosynthesis in diverse nonribosomal peptide pathways. Our work also begins to shed light on the mechanism of C domains. Understanding C domain function may facilitate identification of new natural products that contain dehydroamino acids and enable engineering of dehydroamino acids into nonribosomal peptides.
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http://dx.doi.org/10.1021/jacs.1c13404DOI Listing
July 2022

Conformational Transition-Driven Self-Folding Hydrogel Based on Silk Fibroin and Gelatin for Tissue Engineering Applications.

Macromol Biosci 2022 Jul 27:e2200189. Epub 2022 Jul 27.

Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing, 401331, P. R. China.

Self-folding is a rapidly evolving method for converting flat objects into three-dimensional (3D) structures. However, because there are few materials with suitable properties, the application of self-folding in tissue engineering has been hindered greatly. Herein, a novel self-folding hydrogel using  conformational transition mechanism is developed by employing  photocrosslinkable silk fibroin and gelatin composite hydrogel. It is hypothesized that differences in the amount of beta-sheet (β-sheet) formation between the upper and lower layers will supply additional folding stress and drive the self-folding behavior of a bilayer patch, which can improve the mechanical properties and long-term stability of the self-folded structure. In this study, the impact of various proportions of β-sheets in composite hydrogels on their swelling, mechanics, and internal microstructures are investigated. Subsequently, the folding process parameters are optimized, and diffusion through the folded tubular structure is studied with a perfusion test. Finally, it is proven that the self-folding hydrogel system is cytocompatible and can be utilized to build a 3D coculture system of "endothelial cells-smooth muscle cells". These findings suggest that the self-folding hydrogel can be a promising candidate for applications in blood vessel tissue engineering and regenerative medicine.
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http://dx.doi.org/10.1002/mabi.202200189DOI Listing
July 2022

MSPJ: Discovering potential biomarkers in small gene expression datasets ensemble learning.

Comput Struct Biotechnol J 2022 14;20:3783-3795. Epub 2022 Jul 14.

Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing 400037, China.

In transcriptomics, differentially expressed genes (DEGs) provide fine-grained phenotypic resolution for comparisons between groups and insights into molecular mechanisms underlying the pathogenesis of complex diseases or phenotypes. The robust detection of DEGs from large datasets is well-established. However, owing to various limitations (e.g., the low availability of samples for some diseases or limited research funding), small sample size is frequently used in experiments. Therefore, methods to screen reliable and stable features are urgently needed for analyses with limited sample size. In this study, MSPJ, a new machine learning approach for identifying DEGs was proposed to mitigate the reduced power and improve the stability of DEG identification in small gene expression datasets. This ensemble learning-based method consists of three algorithms: an improved multiple random sampling with -analysis, SVM-RFE (support vector machines-recursive feature elimination), and permutation test. MSPJ was compared with ten classical methods by 94 simulated datasets and large-scale benchmarking with 165 real datasets. The results showed that, among these methods MSPJ had the best performance in most small gene expression datasets, especially those with sample size below 30. In summary, the MSPJ method enables effective feature selection for robust DEG identification in small transcriptome datasets and is expected to expand research on the molecular mechanisms underlying complex diseases or phenotypes.
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http://dx.doi.org/10.1016/j.csbj.2022.07.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304602PMC
July 2022

RBF Neural Network Sliding Mode Control for Passification of Nonlinear Time-Varying Delay Systems with Application to Offshore Cranes.

Sensors (Basel) 2022 Jul 13;22(14). Epub 2022 Jul 13.

Department of Computing, The Hong Kong Polytechnic University, Hong Kong 999077, China.

This paper is devoted to studying the passivity-based sliding mode control for nonlinear systems and its application to dock cranes through an adaptive neural network approach, where the system suffers from time-varying delay, external disturbance and unknown nonlinearity. First, relying on the generalized Lagrange formula, the mathematical model for the crane system is established. Second, by virtue of an integral-type sliding surface function and the equivalent control theory, a sliding mode dynamic system can be obtained with a satisfactory dynamic property. Third, based on the RBF neural network approach, an adaptive control law is designed to ensure the finite-time existence of sliding motion in the face of unknown nonlinearity. Fourth, feasible easy-checking linear matrix inequality conditions are developed to analyze passification performance of the resulting sliding motion. Finally, a simulation study is provided to confirm the validity of the proposed method.
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http://dx.doi.org/10.3390/s22145253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316302PMC
July 2022

Solid Stress-Distribution-Oriented Design and Topology Optimization of 3D-Printed Heterogeneous Lattice Structures with Light Weight and High Specific Rigidity.

Authors:
Bo Li Ciming Shen

Polymers (Basel) 2022 Jul 9;14(14). Epub 2022 Jul 9.

School of Mechanical and Power Engineering, East China University of Science and Technology, Shanghai 200237, China.

Lightweight structural design is greatly valued in the aviation, aerospace, and automotive industries. Three-dimensional (3D) printing techniques provide viable and popular technical pathways for the rapid design and manufacturing of lightweight lattice structures. Unlike the conventional design idea of a geometrically homogenized lattice structure, this work provides a design method for structurally heterogeneous lattice according to the spatial stress state of 3D-printed parts. Following the quasi-static stress numerical simulations of solid components, finite element mesh units were inconsistently replaced by lattice units with different specific rigidities corresponding to the localized stress levels. Relying on the topology optimization further lightened the lattice structure under quasi-static stress after removing some parts with extremely low stress from the overall structure. As an embodiment of this design idea, face-centered cubic (FCC) lattice units with different strut diameters were employed to non-uniformly and adaptively fill a solid part under localized loading. The topological optimization was conducted on the solid part globally. Then, the topologically optimized solid and the heterogeneous lattice structure were subjected to the geometric Boolean operation. Stereolithographic 3D printing was utilized to fabricate the homogeneous and heterogeneous lattice structural parts for comparative tests of three-point bending. Three evaluation indicators were defined for the standardized assessment of the geometrically complex lattice structures for the performance evaluation. This demonstrated that the heterogeneous lattice part exhibited better comprehensive mechanical performance than the uniform lattice. This work proved the feasibility of this new perspective on 3D-printed lightweight structure design and topology optimization.
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http://dx.doi.org/10.3390/polym14142807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319585PMC
July 2022

A Novel Based-Network Strategy to Identify Phytochemicals from Radix Salviae Miltiorrhizae (Danshen) for Treating Alzheimer's Disease.

Molecules 2022 Jul 12;27(14). Epub 2022 Jul 12.

State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Alzheimer's disease (AD) is a common age-related neurodegenerative disease that strikes millions worldwide. Herein, we demonstrate a new approach based on network target to identify anti-AD compounds from Danshen. Network pharmacology and molecular docking were employed to establish the DS-AD network, which mainly involved apoptosis of neuron cells. Then network scoring was confirmed via Connectivity Map analysis. M308 (Danshenxinkun D) was an anti-AD candidate with a high score ( < 0.01). Furthermore, we conducted ex vivo experiments with HO-treated PC12 cells to verify the neuroprotective effect of Salvia miltiorrhiza-containing plasma (SMP), and UPLC-Q-TOF/MS and RT-qPCR were performed to demonstrate the anti-AD activity of M308 from SMP. Results revealed that SMP could enhance cell viability and level of acetylcholine. AO/EB staining and Mitochondrial membrane potential (MMP) analysis showed that SMP significantly suppressed apoptosis, which may be due to anti-oxidative stress activity. Moreover, the effects of M308 and SMP on expressions of PSEN1, DRD2, and APP mRNA were consistent, and M308 can significantly reverse the expression of PSEN1 and DRD2 mRNA in HO-treated PC12 cells. The strategy based on the network could be employed to identify anti-AD compounds from Chinese herbs. Notably, M308 stands out as a promising anti-AD candidate for development.
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http://dx.doi.org/10.3390/molecules27144463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317794PMC
July 2022

Prognostic Value of Systemic Immune-Inflammation Index among Critically Ill Patients with Acute Kidney Injury: A Retrospective Cohort Study.

J Clin Med 2022 Jul 8;11(14). Epub 2022 Jul 8.

Department of Kidney Disease and Blood Purification, Institute of Urology & Key Laboratory of Tianjin, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Inflammation plays a significant role in the occurrence and development of acute kidney injury (AKI). Evidence regarding the prognostic effect of the systemic immune-inflammation index (SII) in critically ill patients with AKI is scarce. The aim of this study was to assess the association between SII and all-cause mortality in these patients. Detailed clinical data were extracted from the Medical Information Mart for Intensive Care Database (MIMIC)-IV. The primary outcome was set as the in-hospital mortality. A total of 10,764 AKI patients were enrolled in this study. The restricted cubic splines analyses showed a J-shaped curve between SII and the risk of in-hospital and ICU mortality. After adjusting for relevant confounders, multivariate Cox regression analysis showed that both lower and higher SII levels were associated with an elevated risk of in-hospital all-cause mortality. A similar trend was observed for ICU mortality. In summary, we found that the SII was associated in a J-shaped pattern with all-cause mortality among critically ill patients with AKI. SII appears to be have potential applications in the clinical setting as a novel and easily accessible biomarker for predicting the prognosis of AKI patients.
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http://dx.doi.org/10.3390/jcm11143978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319546PMC
July 2022

Soil Chemical Properties, Metabolome, and Metabarcoding Give the New Insights into the Soil Transforming Process of Fairy Ring Fungi .

J Fungi (Basel) 2022 Jun 28;8(7). Epub 2022 Jun 28.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, 100 Daxue Rd., Nanning 530004, China.

A unique ecological landscape distributed in the Mongolian Plateau, called fairy rings, caused by the growth of the fungus (LM) in the soil could promote plant growth without fertilization. Therefore, this landscape can alleviate fertilizer use and has excellent value for agricultural production. The previous studies only investigated several parameters of the fairy rings, such as soil microbial diversity and some soil chemical properties, thus conclusions based on the studies on fairy rings lack comprehension. Therefore, the present study systematically investigated the chemical properties, metabolome, and metabarcoding of LM-transformed soil. We analyzed fairy ring soils from DARK (FR) and OUT (CK) zone correlated growth promotion with ten soil chemical properties, including N, nitrate-N, inorganic-P, cellulose, available boron, available sulfur, Fe, Mn, Zn, and Cu, which were identified as important markers to screen fairy ring landscapes. Metabolomics showed that the accumulation of 17 carbohydrate-dominated metabolites was closely associated with plant growth promotion. Finally, metabarcoding detected fungi as the main components affecting soil conversion. Among the various fungi at the family level, Lasiosphaeriaceae, unidentified_Auriculariales_sp, and Herpotrichiellaceae were markers to screen fairy ring. Our study is novel and systematically reveals the fairy ring soil ecology and lists the key factors promoting plant growth. These findings lay a theoretical foundation for developing the fairy ring landscape in an agricultural system.
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http://dx.doi.org/10.3390/jof8070680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324422PMC
June 2022

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.

Brain Sci 2022 Jul 15;12(7). Epub 2022 Jul 15.

Department of Emergency Medicine, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China.

Predicting neurological outcomes after cardiac arrest remains a major issue. This study aimed to identify novel biomarkers capable of predicting neurological prognosis after cardiac arrest. Expression profiles of GSE29540 and GSE92696 were downloaded from the Gene Expression Omnibus (GEO) database to obtain differentially expressed genes (DEGs) between high and low brain performance category (CPC) scoring subgroups. Weighted gene co-expression network analysis (WGCNA) was used to screen key gene modules and crossover genes in these datasets. The protein-protein interaction (PPI) network of crossover genes was constructed from the STRING database. Based on the PPI network, the most important hub genes were identified by the cytoHubba plugin of Cytoscape software. Eight hub genes (RPL27, EEF1B2, PFDN5, RBX1, PSMD14, HINT1, SNRPD2, and RPL26) were finally screened and validated, which were downregulated in the group with poor neurological prognosis. In addition, GSEA identified critical pathways associated with these genes. Finally, a Pearson correlation analysis showed that the mRNA expression of hub genes EEF1B2, PSMD14, RPFDN5, RBX1, and SNRPD2 were significantly and positively correlated with NDS scores in rats. Our work could provide comprehensive insights into understanding pathogenesis and potential new biomarkers for predicting neurological outcomes after cardiac arrest.
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http://dx.doi.org/10.3390/brainsci12070928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316619PMC
July 2022
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