Publications by authors named "Bo Ding"

257 Publications

The effects of hsa_circ_0000517/miR-326 axis on the progression of breast cancer cells and the prediction of miR-326 downstream targets in breast cancer.

Pathol Res Pract 2021 Sep 29;227:153638. Epub 2021 Sep 29.

Department of Radiology, The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an, Huai'an 223002, China. Electronic address:

It has been proposed that circular RNAs (circRNAs) play crucial roles in the initiation and progression of various cancers including breast cancer. Our study aimed to determine the function and regulatory mechanism of hsa_circ_0000517 in breast cancer. qRT-PCR was applied to determine hsa_circ_0000517 expression in breast cancer cells. The circular structure of hsa_circ_0000517 was confirmed using RNase R digestion assay. The subcellular distribution of hsa_circ_0000517 was analyzed using nuclear mass separation assay. Effects of hsa_circ_0000517 on the malignant behaviors of breast cancer cells were determined using CCK-8, colony formation assay, flow cytometry analysis, caspase-3 activity assay, and Transwell invasion assay. Bioinformatics analysis, luciferase reporter assay, and RIP were used to predict and confirm the interaction between hsa_circ_0000517 and miR-326. Bioinformatics analysis was used to search the possible targets of miR-326. Hsa_circ_0000517 was upregulated in breast cancer tissues and cells. Hsa_circ_0000517 was a stable circularized transcript that was preferentially distributed in the cytoplasm. Hsa_circ_0000517 knockdown inhibited cell proliferation, colony formation ability, and invasion and triggered apoptosis in breast cancer cells. Hsa_circ_0000517 acted as a sponge of miR-326 to suppress its expression. miR-326 inhibition abolished the effects of hsa_circ_0000517 knockdown on the malignant behaviors of breast cancer cells. Totally 17 genes were identified as the potential targets of miR-326 in breast cancer. In conclusion, hsa_circ_0000517 silencing repressed breast cancer progression by upregulating miR-326 expression.
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http://dx.doi.org/10.1016/j.prp.2021.153638DOI Listing
September 2021

Effectiveness of self-myofascial release combined with biofeedback and electrical stimulation for the management of myofascial pelvic pain: A randomized controlled trial.

Eur J Pain 2021 Sep 30. Epub 2021 Sep 30.

Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, China.

Background: Myofascial pelvic pain (MFPP) caused by myofascial trigger points (MTrPs) is a major contributor to chronic pelvic pain in women. However, the effect of the patient's self-myofascial release (SMFR) is unclear. This study aimed to investigate the effect of SMFR combined with biofeedback and electrical stimulation (BES) therapy in comparison with BES alone in patients with MFPP.

Methods: A prospective randomized controlled study was conducted. Sixty-eight patients were randomly allocated into BES-SMFR group (n = 34) and BES group (n = 34). Every patient received 4 weeks of treatment, evaluated at baseline (T0), 4 weeks post-intervention (T4) and 12-week follow-up (T12). The primary outcome was pain intensity. The secondary outcomes were degree of activation of MTrPs, surface electromyography (sEMG) levels and Patient Global Impression of Improvement (PGI-I).

Results: Compared with the effect of BES, BES-SMFR treatment significantly decreased pain intensity and the degree of activation of MTrPs in the levator ani (p = 0.02) and obturator internus (p = 0.03), as well as the sEMG levels of the pre-test resting baseline and post-test resting baseline (all p < 0.01). The degree of activation of MTrPs in the piriformis and coccygeus (all p > 0.05) and the sEMG levels of the quick flicks and endurance contraction were not significantly different. The BES-SMFR treatment improved the PGI-I scale at T4 (p = 0.02) but not at T12 (p = 0.40).

Conclusions: This study confirmed that the addition of SMFR to BES treatment resulted in superior outcomes compared with those with BES alone in patients with MFPP.

Significance Statement: Myofascial pelvic pain (MFPP) is a major contributor of female chronic pelvic pain. Myofascial release has been used commonly for better pain release; however, poor therapeutic effect due to poor patient compliance is common in clinical practice. Therefore, in future research, there is a need to investigate the effect of patient's self-myofascial release (SMFR) technique, which can eliminate the need for frequent office visits and improve patient compliance to some extent, in patients with MFPP.
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http://dx.doi.org/10.1002/ejp.1867DOI Listing
September 2021

Laparoscopic radical hysterectomy with enclosed colpotomy without the use of uterine manipulator for early-stage cervical cancer.

J Minim Access Surg 2021 Oct-Dec;17(4):570-572

Department of Obstetrics and Gynecoloty, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

Background: We report the application of transuterine suspension sutures (TUSSs) for manipulation and vaginal closure before colpotomy in laparoscopic radical hysterectomy for early-stage cervical cancer.

Methodology: Eight patients with clinical stage IB1 cervical squamous cell cancer were treated with laparoscopic radical hysterectomy between October 2019 and May 2020. The procedure was performed without a traditional uterine manipulator, and the vaginal cuff was closed with a stapler before colpotomy to prevent tumour spillage.

Results: All patients successfully underwent the surgery, with a median hospitalisation of 8 days (range 6-14). All drains and urethral catheters were removed after a median of 7 days (range 5-11) and 16 days (range 12-21), respectively. A median of 26 (range 20-32) pelvic lymph nodes were resected and no lymph-related complications were encountered post-operatively. With an enclosed colpotomy, no visible tumour tissues were exposed to the pelvic cavity, and all vaginal stumps healed well without complications. All pathological examinations of the vaginal margin were negative, and there were no residual lesions. At a median follow-up of 6 months, all patients were alive with no recurrence of disease.

Conclusion: We found that laparoscopic radical hysterectomy with TUSS and vaginal closure before colpotomy is a useful and effective procedure to prevent tumour spillage for the treatment of cervical cancer.
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http://dx.doi.org/10.4103/jmas.JMAS_146_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486072PMC
September 2021

Single-cell-type transcriptomic analysis reveals distinct gene expression profiles in wheat guard cells in response to abscisic acid.

Funct Plant Biol 2021 Oct;48(11):1087-1099

International Joint Center for the Mechanismic Dissection and Genetic Improvement of Crop Stress Tolerance, College of Agriculture & Resources and Environmental Sciences, Tianjin Agricultural University, Tianjin 300392, China.

Stomatal closure, driven by shrinking guard cells in response to the accumulation of abscisic acid (ABA) under drought stress, has a great impact on plant growth and environmental acclimation. However, the molecular regulatory mechanism underlying the turgor alteration of guard cells remains elusive, especially in cereal grasses. Here, we develop a modified enzyme digestion-based approach for the isolation of wheat (Triticum aestivum L.) guard cells. With this approach, we can remove mesophyll, pavement cells and subsidiary cells successively from the epidermis of the trichomeless coleoptile in wheat and preserve guard cells on the cuticle layers in an intact and physiologically active conditions. Using a robust single-cell-type RNA sequencing analysis, we discovered 9829 differentially expressed genes (DEGs) as significantly up- or down-regulated in guard cells in response to ABA treatment. Transcriptome analysis revealed a large percent of DEGs encoding multiple phytohormone signalling pathways, transporters, calcium signalling components, protein kinases and other ABA signalling-related proteins, which are primarily involved in key signalling pathways in ABA-regulated stomatal control and stress response. Our findings provide valuable resource for investigating the transcriptional regulatory mechanism underlying wheat guard cells in response to ABA.
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http://dx.doi.org/10.1071/FP20368DOI Listing
October 2021

Association of Acarbose with Decreased Muscle Mass and Function in Patients with Type 2 Diabetes: A Retrospective, Cross-Sectional Study.

Diabetes Ther 2021 Nov 20;12(11):2955-2969. Epub 2021 Sep 20.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, No. 32 Gongqingtuan Road, Nanjing, 210012, Jiangsu, China.

Introduction: Prevalence of sarcopenia has increased in patients with type 2 diabetes. The influence of glucose-lowering drugs on muscles in these patients remains unclear. We aimed to investigate the association between muscle mass/function and glucose-lowering drugs.

Methods: Data of 1042 hospitalized patients with type 2 diabetes were included in this retrospective, cross-sectional study. All the patients had stable hypoglycemic therapy in the last 3 months, and performed bioelectrical impedance analysis, grip strength, and gait speed tests on admission.

Results: Skeletal muscle index [6.81 (95% CI 6.67, 6.94) vs. 7.17 (7.09, 7.24) kg/m], handgrip strength [23.41 (22.24, 24.58) vs. 26.93 (26.33, 27.54) kg], and gait speed [1.19 (1.15, 1.22) vs. 1.27 (1.25, 1.28) m/s] decreased in patients using acarbose compared with the others (all p < 0.001). Gait speed and skeletal muscle index remained lower in patients using acarbose compared to their matched patients in propensity score matching (p = 0.036 and 0.010, respectively). Among drug-naïve patients and patients using insulin, metformin, sulfonylureas, or acarbose monotherapy, the acarbose group had lowest skeletal muscle index and handgrip strength [6.81 (6.52, 7.11) kg/m and 22.54 (19.28, 25.79) kg, p = 0.028 and 0.001, respectively].

Conclusion: Acarbose treatment was associated with decreased muscle mass and strength. Assessment and exercise of muscles in patients with long-term acarbose treatment should be considered.
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http://dx.doi.org/10.1007/s13300-021-01151-6DOI Listing
November 2021

Characteristics of childhood allergic diseases in outpatient and emergency departments in Shanghai, China, 2016-2018: a multicenter, retrospective study.

BMC Pediatr 2021 09 17;21(1):409. Epub 2021 Sep 17.

Department of Respiratory Medicine, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201102, China.

Background: The prevalence of allergic diseases (ADs), such as asthma and allergic rhinitis (AR), is increasing worldwide in both adults and children. Although ADs are common and frequently coexist in outpatient care, city-level data regarding the characteristics of childhood AD remain limited in China. This study aimed to assess the profile and characteristics of ADs in the city of Shanghai.

Methods: A multicenter retrospective study was designed to collect routine administrative data from outpatient and emergency departments from 66 hospitals in Shanghai, China, from 2016 to 2018. Children with asthma, AR, allergic conjunctivitis (AC), and allergic skin diseases were investigated. Demographic characteristics, patients visit pattern, spectrum of diagnosis, and comorbidities were analyzed.

Results: A total of 2,376,150 outpatient and emergency visits for ADs were included in the period from 2016 to 2018. Allergic skin diseases accounted for 38.9%, followed by asthma (34.8%), AR (22.9%), and AC (3.3%), with a male predominance in all four diseases. Asthma and allergic skin diseases were most frequent in the 1 to < 4 years of age group, while AR and AC were more common in the 4 to < 7 years of age group. Asthma accounted for the greatest number of annual and emergency visits. The most frequent comorbidity of asthma was lower respiratory tract infection (LRTI) (49.3%), followed by AR (20.5%) and upper respiratory tract infection (14.1%). The most common comorbidities of AR were otitis media (23.4%), adenoid hypertrophy/obstructive sleep apnea (22.1%), followed by LRTI (12.1%), asthma (9.4%) and chronic pharyngitis (8.9%).

Conclusions: Asthma and allergic skin diseases were the most common ADs in outpatient and emergency departments in the study period. Respiratory tract infection was the most common comorbidity of asthma in children. More attention should be devoted to the treatment of comorbidities to improve childhood AD outcomes with a better understanding of the characteristics of ADs in outpatient care.
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http://dx.doi.org/10.1186/s12887-021-02880-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447671PMC
September 2021

KnowRU: Knowledge Reuse via Knowledge Distillation in Multi-Agent Reinforcement Learning.

Entropy (Basel) 2021 Aug 13;23(8). Epub 2021 Aug 13.

College of Computer, National University of Defense Technology, Changsha 410000, China.

Recently, deep reinforcement learning (RL) algorithms have achieved significant progress in the multi-agent domain. However, training for increasingly complex tasks would be time-consuming and resource intensive. To alleviate this problem, efficient leveraging of historical experience is essential, which is under-explored in previous studies because most existing methods fail to achieve this goal in a continuously dynamic system owing to their complicated design. In this paper, we propose a method for knowledge reuse called "KnowRU", which can be easily deployed in the majority of multi-agent reinforcement learning (MARL) algorithms without requiring complicated hand-coded design. We employ the knowledge distillation paradigm to transfer knowledge among agents to shorten the training phase for new tasks while improving the asymptotic performance of agents. To empirically demonstrate the robustness and effectiveness of KnowRU, we perform extensive experiments on state-of-the-art MARL algorithms in collaborative and competitive scenarios. The results show that KnowRU outperforms recently reported methods and not only successfully accelerates the training phase, but also improves the training performance, emphasizing the importance of the proposed knowledge reuse for MARL.
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http://dx.doi.org/10.3390/e23081043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393270PMC
August 2021

Potent Anticancer Activities of Beauvericin against KB cells In Vitro by Inhibiting the Expression of ACAT1 and Exploring Binding Affinity.

Anticancer Agents Med Chem 2021 Aug 5. Epub 2021 Aug 5.

Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436. China.

Background And Objective: Beauvericin (BEA), a cyclic hexadepsipeptide mycotoxin, is a potent inhibitor of the acyl-CoA: cholesterol acyltransferase enzyme 1 (ACAT1) which involved in multiple tumor-correlated pathways. However, the binding mechanisms between BEA and ACAT1 were not elucidated.

Methods: BEA was purified from a mangrove entophytic Fusarium sp. KL11. Single-crystal X-ray diffraction was used to determine the structure of BEA. Wound healing assays of BEA against KB cell line and MDA-MB-231 cell line were evaluated. Inhibitory potency of BEA against ACAT1 was determined by ELISA assays. Molecular docking was carried out to illuminate the bonding mechanism between BEA and ACAT1.

Results: The structure of BEA was confirmed by X-ray diffraction, indicating a monoclinic crystal system with P21 space group (α = 90°, β = 92.2216(9)o, γ= 90o). BEA displayed migration-inhibitory activities against KB cells and MDA-MB-231 cells in vitro. ELISA assays revealed the protein expression level of ACAT1 in KB cells was significantly decreased after BEA treatment (P <0.05). Molecular docking demonstrated that BEA formed hydrogen bond with His425 and pi-pi staking with Tyr429 in ACAT1.

Conclusions: BEA sufficiently inhibited the proliferation and migration of KB cells and MDA-MB-231 cells by downregulating ACAT1 expression. In addition, BEA potentially possessed a strong binding affinity with ACAT1. BEA may serve as a potential lead compound for the development of a new ACAT1-targeted anticancer drug.
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http://dx.doi.org/10.2174/1871520621666210805123739DOI Listing
August 2021

Alterations in the activity and sleep of Drosophila melanogaster under simulated microgravity.

NPJ Microgravity 2021 Jul 22;7(1):27. Epub 2021 Jul 22.

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.

This study aimed to investigate alterations in the activity and sleep of Drosophila melanogaster under simulated microgravity, which was implemented through the random positioning machine, while different light conditions (normal photoperiod and constant dark) were set. Fruit flies of different strains and sexes were treated for 3 days, and activity and sleep were monitored using the Drosophila Activity Monitoring System. After 3 days of treatment, fruit flies were sampled to detect the relative expression levels of the major clock genes and some neurotransmitter-related genes. The results showed that for the normal photoperiod (LD) condition, the activity increased and sleep decreased under simulated microgravity, while for the constant dark (DD) condition, the activity and sleep rhythms appeared disordered and the activity increased, thus decreasing the likelihood of waking up during the day. Light conditions, strains, and sexes, individually or in combination, had impacts on the simulated microgravity effects on behaviors. The clock genes and neurotransmitter-related genes had different degrees of response among sexes and strains, although the overall changes were slight. The results indicated that the normal photoperiod could ease the effects of simulated microgravity on fruit flies' activity and sleep and possible unidentified pathways involved in the regulatory mechanism need further exploration. This study is expected to provide ideas and references for studying the effects of microgravity on space life science.
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http://dx.doi.org/10.1038/s41526-021-00157-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298474PMC
July 2021

Associations of fine particulate matter and constituents with pediatric emergency room visits for respiratory diseases in Shanghai, China.

Int J Hyg Environ Health 2021 07 13;236:113805. Epub 2021 Jul 13.

School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China. Electronic address:

Background: Although ambient fine particulate matter (PM) has been associated with adverse respiratory outcomes in children, few studies have examined PM constituents with respiratory diseases in children in China.

Objectives: To investigate the associations of short-term exposure to PM and its constituents with pediatric emergency room visits (ERVs) for respiratory diseases in Shanghai, China.

Methods: We collected daily concentrations of PM and its constituents in urban Shanghai from January 1, 2016, to December 31, 2018. Daily pediatric ERVs for four major respiratory diseases, including upper respiratory tract infection, bronchitis, pneumonia, and asthma, were obtained from 66 hospitals in Shanghai during the same period. Associations of exposure to daily PM and constituents with respiratory ERVs were estimated using the over-dispersed generalized additive models.

Result: Short-term exposure to PM and its constituents were associated with increased pediatric ERVs for respiratory diseases. Specifically, an interquartile range increase in the 3-day average PM level (31 μg/m) was associated with 1.86% (95%CI: 0.52, 3.22), 1.53% (95%CI: 0.01, 3.08), 1.90% (95%CI: 0.30, 3.52), and 2.67% (95%CI: 0.70, 4.68) increase of upper respiratory tract infection, bronchitis, pneumonia, and asthma ERVs, respectively. As for PM constituents, we found organic carbon, ammonium, nitrate, selenium, and zinc were associated with higher risk of respiratory ERVs in the single constituent and the constituent-PM models.

Conclusion: Short-term exposure to PM was associated with increased pediatric ERVs for respiratory diseases. Constituents related to anthropogenic combustion and traffic might be the dominant contributors of the observed associations.
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http://dx.doi.org/10.1016/j.ijheh.2021.113805DOI Listing
July 2021

Serum albumin and albuminuria predict the progression of chronic kidney disease in patients with newly diagnosed type 2 diabetes: a retrospective study.

PeerJ 2021 6;9:e11735. Epub 2021 Jul 6.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.

Background: Diabetes-related kidney disease is associated with end-stage renal disease and a high mortality rate. However, data on risk factors associated with kidney disease in patients with newly diagnosed type 2 diabetes mellitus (DM) remains insufficient. The aim of the present study was to identify the risk factors significantly associated with chronic kidney disease progression in patients with newly diagnosed type 2 DM.

Methods: We reviewed a total of 254 consecutive patients who were newly diagnosed with type 2 diabetes at Nanjing First Hospital from January to December 2014. They were observed for two years, and baseline and biochemical variables were used to identify significant predictors of kidney failure progression. Kidney failure progression was defined as a ≥ 30% increase in serum creatine level.

Results: The mean age of patients was 58.96 years, 37.4% were women, and 57.1% had hypertension. Kidney function progressed in 40 patients (15.75%). Multivariable logistic regression analyses showed that serum albumin ( = 0.015) and microalbuminuria ( < 0.001) were associated with kidney failure progression in patients with newly diagnosed type 2 DM. Those with lower estimated glomerular filtration rate (eGFR; 30-60 ml/min/1.73 m) at baseline had lower serum albumin levels compared to those of patients with higher eGFR. The albuminuria levels were higher in patients with lower eGFR than in those with eGFR ≥ 90 ml/min/1.73 m. Receiver operating characteristic curve analysis showed that the area under the curve was 0.754 (95% CI [0.670-0. 0.837]).

Conclusions: The overall rate of chronic kidney disease progression is relatively high, and low serum albumin and high albuminuria levels are associated with kidney failure progression in newly diagnosed diabetic patients.
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http://dx.doi.org/10.7717/peerj.11735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269640PMC
July 2021

Identification of autophagy-related risk signatures for the prognosis, diagnosis, and targeted therapy in cervical cancer.

Cancer Cell Int 2021 Jul 8;21(1):362. Epub 2021 Jul 8.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, 87 Dingjiaqiao, Gulou District, Nanjing, 210009, China.

Background: To rummage autophagy-related prognostic, diagnostic, and therapeutic biomarkers in cervical cancer (CC).

Methods: The RNA-sequence and clinical information were from the TCGA and GTEx databases. We operated Cox regression to determine signatures related to overall survival (OS) and recurrence-free survival (RFS) respectively. The diagnostic and therapeutic effectiveness of prognostic biomarkers were further explored.

Results: We identified nine (VAMP7, MTMR14, ATG4D, KLHL24, TP73, NAMPT, CD46, HGS, ATG4C) and three risk signatures (SERPINA1, HSPB8, SUPT20H) with prognostic values for OS and RFS respectively. Six risk signatures (ATG4C, ATG4D, CD46, TP73, SERPINA1, HSPB8) were selected for qPCR. We screened five prognostic signatures(ATG4C, CD46, HSPB8, MTMR14, NAMPT) with diagnostic function through the GEO database. Correlation between our models and treatment targets certificated the prognostic score provided a reference for precision medicine.

Conclusions: We constructed OS and RFS prognostic models in CC. Autophagy-related risk signatures might serve as diagnostic and therapeutic biomarkers.
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http://dx.doi.org/10.1186/s12935-021-02073-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268251PMC
July 2021

Removal of organic contaminants from wastewater with GO/MOFs composites.

PLoS One 2021 8;16(7):e0253500. Epub 2021 Jul 8.

State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body, College of Mechanical and Vehicle Engineering, Hunan University, Changsha City, P.R. China.

Graphene oxide/metal-organic frameworks (GO/MOFs) have been prepared via solvothermal synthesis with ferrous sulfate heptahydrate, zirconium acetate and terephthalic acid for the purpose of removing organic pollutants from wastewater. The composites were analyzed using scanning electron microscopy, infrared spectrometry, and XRD. Tetracycline hydrochloride and orange II were implemented as model pollutants to evaluate the efficacy of the GO/MOFs in water purification, in which 50 mg of Zr/Fe-MOFs/GO was mixed with 100 mL of 10 mg/L, 20 mg/L, 30 mg/L, or 50 mg/L tetracycline hydrochloride solution and 25 mg/L, 35 mg/L, 45 mg/L, or 60 mg/L orange II solution, respectively. The removal efficacy after 4 hours was determined to be 96.1%, 75.8%, 55.4%, and 30.1%, and 98.8%, 91.9%, 71.1%, and 66.2%, respectively. The kinetics of pollutant removal was investigated for both tetracycline hydrochloride and orange II and excellent correlation coefficients of greater than 0.99 were obtained. The high efficacy of these MOFs in pollutant removal, coupled with their inexpensive preparation indicates the feasibility of their implementation in strategies for treating waste liquid. As such, it is anticipated that Zr/Fe-MOFs/GO composites will be widely applied in wastewater purification.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253500PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266086PMC
July 2021

[Advances in development and application of liquid chromatography coupled with isotope ratio mass spectrometry].

Se Pu 2020 Jun;38(6):627-638

Qinghai Province Association of Ophiocordyceps sinensis, Xining 810007, China.

The first commercial interface for hyphenating liquid chromatography with isotope ratio mass spectrometry (LC-IRMS) was not available until 2004, and it was commercialized under the name LC IsoLink by Thermo Finnigan. LC-IRMS, a method of compound-specific isotope analysis, has enabled the detection of stable carbon isotope ratios (C) in targeted substances in order to determine their origin and authenticity. This paper provides an overview of IRMS and LC-IRMS techniques, the history and detailed classification of the latter over the past twenty years. The application of LC-IRMS to the fields of food safety, environment and ecology, life sciences, and archeology are reviewed. Finally, the technical limitations, current challenges, and future development trends of LC-IRMS are outlined.
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http://dx.doi.org/10.3724/SP.J.1123.2019.12007DOI Listing
June 2020

Roles of Key Ion Channels and Transport Proteins in Age-Related Hearing Loss.

Int J Mol Sci 2021 Jun 7;22(11). Epub 2021 Jun 7.

Department of Medical Engineering, College of Engineering, University of South Florida, Tampa, FL 33620, USA.

The auditory system is a fascinating sensory organ that overall, converts sound signals to electrical signals of the nervous system. Initially, sound energy is converted to mechanical energy via amplification processes in the middle ear, followed by transduction of mechanical movements of the oval window into electrochemical signals in the cochlear hair cells, and finally, neural signals travel to the central auditory system, via the auditory division of the 8th cranial nerve. The majority of people above 60 years have some form of age-related hearing loss, also known as presbycusis. However, the biological mechanisms of presbycusis are complex and not yet fully delineated. In the present article, we highlight ion channels and transport proteins, which are integral for the proper functioning of the auditory system, facilitating the diffusion of various ions across auditory structures for signal transduction and processing. Like most other physiological systems, hearing abilities decline with age, hence, it is imperative to fully understand inner ear aging changes, so ion channel functions should be further investigated in the aging cochlea. In this review article, we discuss key various ion channels in the auditory system and how their functions change with age. Understanding the roles of ion channels in auditory processing could enhance the development of potential biotherapies for age-related hearing loss.
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http://dx.doi.org/10.3390/ijms22116158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201059PMC
June 2021

[Oncolytic vaccinia virus expressing CD40L (CD40L-VV) inhibits colorectal cancer cell growth and enhances anti-tumor activity of T cells in tumor-bearing mice].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jul;37(7):602-607

Department of Gastroenterological Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China.

Objective To explore the anti-tumor activity of oncolytic vaccinia virus expressing CD40L (CD40L-VV) against colorectal cancer. Methods The CD40L-VV was obtained by integrating the sequence of CD40L into the skeleton of oncolytic vaccinia virus(VV). The tumor cells were infected with VV and CD40L-VV to verify their oncolytic activity and the expression of CD40L in vitro. After the tumor model of colorectal cancer was treated with VV and CD40L-VV, the tumor growth was monitored, and the phenotype of tumor infiltrating T cells was analyzed by flow cytometry. The anti-tumor activity of recombinant oncolytic VV was also reflected by detecting the production of cytokine and the proliferation activity of tumor infiltrating T cells. Results Microscopic observation and Western blot assay showed that CD40L-VV could effectively infect tumor cells and express CD40L. Cell viability assay showed that VV and CD40L-VV had dose-dependent lytic ability against tumor cells. The results of tumor transplantation in vivo showed that CD40L-VV had stronger ability to inhibiting tumor growth than VV. Flow cytometry showed that tumor infiltrating T cells in the CD40L-VV group had stronger cytokine secretion ability, stronger proliferative activity and more memory cell phenotypes than those in the VV group. Conclusion CD40L-VV can significantly inhibit the growth of colorectal cancer cells and enhance the anti-tumor activity of T cells in vivo.
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July 2021

Effectiveness of beinaglutide in a patient with late dumping syndrome after gastrectomy: A case report.

Medicine (Baltimore) 2021 May;100(21):e26086

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Jiangsu, China.

Rationale: Dumping syndrome is a frequent and potentially severe complication after gastric surgery. Beinaglutide, a recombinant human glucagon-like peptide-1 (GLP-1) which shares 100% homology with human GLP-1(7-36), has never been reported in the treatment of dumping syndrome before.

Patient Concerns: The patient had undergone distal gastrectomy for gastric signet ring cell carcinoma 16 months ago. He presented with symptoms of paroxysmal palpitation, sweating, and dizziness for 4 months.

Diagnosis: He was diagnosed with late dumping syndrome.

Interventions And Outcomes: The patient was treated with dietary changes and acarbose for 4 months before admitted to our hospital. The treatment with dietary changes and acarbose did not prevent postprandial hyperinsulinemia and hypoglycemia according to the 75 g oral glucose tolerance test (OGTT) and continuous glucose monitoring (CGM) on admission.Therefore, the patient was treated with beinaglutide 0.1 mg before breakfast and lunch instead of acarbose. After the treatment of beinaglutide for 1 month, OGTT showed a reduction in postprandial hyperinsulinemia compared with before starting treatment, and the time in the range of 3.9 to 10 mmol/L became 100% in CGM. No side effect was observed in this patient during beinaglutide treatment.

Lessons: These findings suggest that beinaglutide may be effective for treating post-gastrectomy late dumping syndrome.
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http://dx.doi.org/10.1097/MD.0000000000026086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154494PMC
May 2021

Identification of an immune signature to predict poor clinical outcome in cervical cancer.

Epigenomics 2021 Jun 6;13(11):891-907. Epub 2021 May 6.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.

To explore tumor immune microenvironment and identify immune prognostic-related circRNAs in cervical cancer. RNA-seq in combination with bioinformatics were performed to establish a prognostic risk model and a circRNAs-miRNAs- network. High-risk group correlated with poor survival outcome, and had lower immunogenicity. Additionally, could distinguish normal tissue, low- and high-risk tumor tissues, the expression of which showed an increasing trend among the three groups. RNA-seq and bioinformatics indicated that circRNAs like might upregulate through sponging miRNAs including . We constructed an immune risk model related with CD8 T cells to predict the cervical cancer patients' prognosis and explored the abnormal expression mechanism of through the ceRNA mechanism.
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http://dx.doi.org/10.2217/epi-2020-0437DOI Listing
June 2021

Simulated mobile communication frequencies (3.5 GHz) emitted by a signal generator affects the sleep of Drosophila melanogaster.

Environ Pollut 2021 Aug 5;283:117087. Epub 2021 Apr 5.

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China; Xiamen Key Laboratory of Physical Environment, Xiamen, 361021, China; Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address:

With the rapid development of science and technology, 5G technology will be widely used, and biosafety concerns about the effects of 5G radiofrequency radiation on health have been raised. Drosophila melanogaster was selected as the model organism for our study, in which a 3.5 GHz radiofrequency radiation (RF-EMR) environment was simulated at intensities of 0.1 W/m, 1 W/m, and 10 W/m. The activity of parent male and offspring (F1) male flies was measured using a Drosophila activity monitoring system under short-term and long-term 3.5 GHz RF-EMR exposure. Core genes associated with heat stress, the circadian clock and neurotransmitters were detected by QRT-PCR technology, and the contents of GABA and glutamate were detected by UPLC-MS. The results show that short-term RF-EMR exposure increased the activity level and reduced the sleep duration while long-term RF-EMR exposure reduced the activity level and increased the sleep duration of F1 male flies. Under long-term RF-EMR, the expression of heat stress response-related hsp22, hsp26 and hsp70 genes was increased, the expression of circadian clock-related per, cyc, clk, cry, and tim genes was altered, the content of GABA and glutamate was reduced, and the expression levels of synthesis, transport and receptor genes were altered. In conclusion, long-term RF-EMR exposure enhances the heat stress response of offspring flies and then affects the expression of circadian clock and neurotransmitter genes, which leads to decreased activity, prolonged sleep duration, and improved sleep quality.
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http://dx.doi.org/10.1016/j.envpol.2021.117087DOI Listing
August 2021

Kinetic Resolution of Tertiary Benzyl Alcohols via Palladium/Chiral Norbornene Cooperative Catalysis.

Angew Chem Int Ed Engl 2021 06 6;60(23):12824-12828. Epub 2021 May 6.

Sauvage Center for Molecular Sciences, Engineering Research Center of Organosilicon Compounds & Materials (Ministry of Education), College of Chemistry and Molecular Sciences, The Institute for Advanced Studies, Wuhan University, Wuhan, 430072, China.

Herein we report a highly enantioselective kinetic resolution of tertiary benzyl alcohols via palladium/chiral norbornene cooperative catalysis. With simple aryl iodides as the resolution reagent, a wide range of readily available racemic tertiary benzyl alcohols are applicable to this method. Both chiral tertiary benzyl alcohols and benzo[c]chromene products are obtained in good to excellent enantioselectivities (selectivity factor up to 544). The appealing synthetic utility of the obtained enantioenriched tertiary alcohols is demonstrated by the facile preparation of several valuable chiral heterocycles. Preliminary mechanism studies include DFT calculations to explain the origin of enantiodiscrimination and control experiments to uncover the formation of a transient axial chirality during the kinetic resolution step.
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http://dx.doi.org/10.1002/anie.202103428DOI Listing
June 2021

Late-Stage Photoredox C-H Amidation of N-Unprotected Indole Derivatives: Access to -(Indol-2-yl)amides.

Org Lett 2021 04 22;23(7):2710-2714. Epub 2021 Mar 22.

Department of Chemistry, Soochow University, No. 70, Linhsi Road, Shihlin District, Taipei 111002, Taiwan.

The late-stage functionalization of N-unprotected indoles can be useful for modifying low-molecular-weight drugs and bioactive peptides. Whereas indole carboxamides are valuable in pharmaceutical applications, the preparation -(indol-2-yl)amides with similar structures continues to be challenging. Herein we report on visible-light-induced late-stage photoredox C-H amidation with N-unprotected indoles and tryptophan-containing peptides, leading to the formation of -(indol-2-yl)amide derivatives. N-Unprotected indoles and aryloxyamides that contain an electron-withdrawing group could be coupled directly to eosin Y as the photocatalyst by irradiation with a green light-emitting diode at room temperature. Mechanistic studies and density functional theory calculations indicate that the transformation might proceed through the oxidative C-H functionalization of indole with a PS* to PS cycle. This protocol provides a new toolkit for the late-stage modification labeling and peptide-drug conjugation of N-unprotected indole derivatives.
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http://dx.doi.org/10.1021/acs.orglett.1c00609DOI Listing
April 2021

Vitamin A and Its Multi-Effects on Pancreas: Recent Advances and Prospects.

Front Endocrinol (Lausanne) 2021 18;12:620941. Epub 2021 Feb 18.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Vitamin A (VA), which is stored in several forms in most tissues, is required to maintain metabolite homeostasis and other processes, including the visual cycle, energy balance, epithelial cell integrity, and infection resistance. In recent years, VA molecules, also known as retinoids, have been extensively explored and used in the treatment of skin disorders and immune-related tumors. To date, several observational and interventional studies have explored the relationship between VA status and the pathogenesis of diabetes. In particular, VA micronutrients have been shown to regulate pancreatic development, β-cell function, pancreatic innate immune responses, and pancreatic stellate cells phenotypes through multiple mechanisms. However, there are still many problems to be proven or resolved. In this review, we summarize and discuss recent and available evidence on VA biological metabolism in the pancreas. Analysis of the effects of VA on metabolism in the pancreas will contribute to our understanding of the supportive physiological roles of VA in pancreas protection.
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http://dx.doi.org/10.3389/fendo.2021.620941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930481PMC
February 2021

Ultrafine particulate air pollution and pediatric emergency-department visits for main respiratory diseases in Shanghai, China.

Sci Total Environ 2021 Jun 11;775:145777. Epub 2021 Feb 11.

Department of Respiratory Medicine, Children's Hospital of Fudan University, Shanghai 201102, China. Electronic address:

Background: Few studies have explored the short-term effects of ultrafine particles (UFPs, particles < 0.1 μm) air pollution on the exacerbations of pediatric respiratory diseases.

Objectives: We aimed to evaluate short-term association between UFP and emergency-department visits (EDVs) for main pediatric respiratory diseases.

Methods: We collected daily data on UFP and pediatric EDVs for main respiratory diseases [asthma, pneumonia, bronchitis and upper respiratory tract infections (URTI)] from 66 hospitals in Shanghai, China from 2016 to 2018. Generalized additive models combined with polynomial distributed lag models were applied to explore the associations between UFP level and pediatric EDVs for respiratory diseases. We fitted two-pollutant models with criteria air pollutants and performed stratified analyses by gender and age.

Results: UFP was associated with increased EDVs for all respiratory diseases in cumulative lags up to 2 d and 3 d. The greatest risk was found at cumulative lags (0-2 d) for all respiratory diseases. At cumulative lags (0-2 d), an interquartile range increase in concentrations of UFP (1800 particles/cm) was associated with relative risks of EDVs due to asthma [1.35, 95% confidence interval (CI): 1.14-1.59], pneumonia (1.20, 95% CI: 1.04-1.38), bronchitis (1.17, 95% CI: 1.03-1.33) and URTI (1.14, 95% CI: 1.02-1.28). These associations were almost unchanged when controlling for criteria air pollutants, and there was no threshold below which the associations were not present. There were stronger associations in children aged 0-13 years.

Conclusions: Short-term exposure to UFP may independently increase the risks of EDVs for asthma, pneumonia, bronchitis and URTI exacerbations among children.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145777DOI Listing
June 2021

Inflammatory cell-derived CXCL3 promotes pancreatic cancer metastasis through a novel myofibroblast-hijacked cancer escape mechanism.

Gut 2021 Feb 10. Epub 2021 Feb 10.

Department of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, Stockholm, Sweden

Objective: Pancreatic ductal adenocarcinoma (PDAC) is the most lethal malignancy and lacks effective treatment. We aimed to understand molecular mechanisms of the intertwined interactions between tumour stromal components in metastasis and to provide a new paradigm for PDAC therapy.

Design: Two unselected cohorts of 154 and 20 patients with PDAC were subjected to correlation between interleukin (IL)-33 and CXCL3 levels and survivals. Unbiased expression profiling, and genetic and pharmacological gain-of-function and loss-of-function approaches were employed to identify molecular signalling in tumour-associated macrophages (TAMs) and myofibroblastic cancer-associated fibroblasts (myoCAFs). The role of the IL-33-ST2-CXCL3-CXCR2 axis in PDAC metastasis was evaluated in three clinically relevant mouse PDAC models.

Results: IL-33 was specifically elevated in human PDACs and positively correlated with tumour inflammation in human patients with PDAC. CXCL3 was highly upregulated in IL-33-stimulated macrophages that were the primary source of CXCL3. CXCL3 was correlated with poor survival in human patients with PDAC. Mechanistically, activation of the IL-33-ST2-MYC pathway attributed to high CXCL3 production. The highest level of CXCL3 was found in PDAC relative to other cancer types and its receptor CXCR2 was almost exclusively expressed in CAFs. Activation of CXCR2 by CXCL3 induced a CAF-to-myoCAF transition and α-smooth muscle actin (α-SMA) was uniquely upregulated by the CXCL3-CXCR2 signalling. Type III collagen was identified as the CXCL3-CXCR2-targeted adhesive molecule responsible for myoCAF-driven PDAC metastasis.

Conclusions: Our work provides novel mechanistic insights into understanding PDAC metastasis by the TAM-CAF interaction and targeting each of these signalling components would provide an attractive and new paradigm for treating pancreatic cancer.
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http://dx.doi.org/10.1136/gutjnl-2020-322744DOI Listing
February 2021

Rapid Changes in Serum Testosterone in Men With Newly Diagnosed Type 2 Diabetes With Intensive Insulin and Metformin.

Diabetes Care 2021 04 3;44(4):1059-1061. Epub 2021 Feb 3.

Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Jiangsu, China

Objective: To investigate the effect of metformin on testosterone levels in men with type 2 diabetes mellitus (T2DM).

Research Design And Methods: Seventy men with newly diagnosed drug-naive T2DM and HbA >9.0% (75 mmol/mol) were treated with intensive insulin pump therapy for 5 days to achieve glucose normalization. They were randomized to control (continued on intensive insulin only) and metformin (plus metformin) groups (1:1) for 1 month. Testosterone was measured at baseline, randomization, and after 1-month treatment.

Results: Total, free, and bioavailable testosterone increased significantly within 5 days (all < 0.001). After 1 month, compared with the control group, the metformin group had lower total (12.7 vs. 15.3 nmol/L), free (0.20 vs. 0.24 nmol/L), and bioavailable (4.56 vs. 5.31 nmol/L) testosterone (all < 0.05).

Conclusions: In men with T2DM, 1-month oral metformin may decrease serum testosterone levels independent of blood glucose control. The effects of long-term metformin on testosterone in men need further study.
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http://dx.doi.org/10.2337/dc20-1558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985426PMC
April 2021

Relevance of STK11 Mutations Regarding Immune Cell Infiltration, Drug Sensitivity, and Cellular Processes in Lung Adenocarcinoma.

Front Oncol 2020 23;10:580027. Epub 2020 Dec 23.

Cardiovascular Surgery Department, Affiliated Hospital of Nantong University, Nantong, China.

Serine/threonine kinase 11 (STK11) is one member of the serine/threonine kinase family, which is involved in regulating cell polarity, apoptosis, and DNA damage repair. In lung adenocarcinoma (LUAD), it can play as one tumor suppressor and always be mutated. In this study, we aimed to assess the relevance of STK11 mutations in LUAD, in which we also studied the correlation among immune cell infiltration, drug sensitivity, and cellular processes. By performing the bioinformatics analysis of the Cancer Genome Atlas (TCGA) about LUAD patients, we found that the mutation efficiency of STK11 mutations is about 19%. Additionally, the differentially expressed gene analysis showed that there were 746 differentially expressed genes (DEGs) between LUAD patients with and without STK11 mutations. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis showed that the DEGs were enriched in various tumorigenesis signaling pathways and metabolic processes. Among these DEGs, the top ranking 21 genes were found that they were more frequently mutated in the STK11 mutation group than in the wild-type group (p-value<0.01). Finally, the LUAD patients with STK11 mutations suffered the worse immune cell infiltration levels than the LUAD patients with wild-type. The STK11 gene copy number was correlated with immune cell infiltration. Aiming to develop the therapeutic drugs, we performed Genomics of Drug Sensitivity in Cancer (GDSC) data to identify the potential therapeutic candidate and the results showed that Nutlin-3a(-) may be a sensitive drug for LUAD cases harboring STK11 mutations. The specific genes and pathways shown to be associated with LUAD cases involving STK11 mutations may serve as targets for individualized LUAD treatment.
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http://dx.doi.org/10.3389/fonc.2020.580027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787160PMC
December 2020

The complete chloroplast genome of (Pentaphylacaceae).

Mitochondrial DNA B Resour 2020 Sep 3;5(3):3311-3312. Epub 2020 Sep 3.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, China.

The complete chloroplast (cp) genome of Dunn. has been reported in this study. The cp genome has a total length of 157,218 bp with the typical quadripartite structure, containing two inverted repeats (IRs) of 25,883 bp separated by a large single-copy (LSC) region of 87,248 bp and a small single-copy (SSC) region of 18,204 bp. The whole cp genome of contains 128 genes, including 83 protein-coding genes, 37 tRNAs genes, and 8 rRNAs. The phylogenetic result showed that is sister to .
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http://dx.doi.org/10.1080/23802359.2020.1814174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717602PMC
September 2020

The complete chloroplast genome of (Plantaginaceae): an endemic and Endangered species from China.

Mitochondrial DNA B Resour 2019 Dec 9;5(1):51-52. Epub 2019 Dec 9.

School of Life Sciences, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Institute of Resources Botany, Southwest University, Chongqing, P. R. China.

This study was the first report about the complete chloroplast genome of (Plantaginaceae). The circular whole cp genome of was in a total length 164,976 bp with the typical quadripartite structure of angiosperms, containing two inverted repeats (IRs) of 38,644 bp separated by a large single-copy (LSC) region and a small single-copy (SSC) region of 82,972 and 4716 bp, respectively. The plastid genome of contains 113 genes, including 79 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. The overall GC content of plastid genome is 38.0% and the corresponding values in LSC, SSC, and IR regions are 36.6, 30.2, and39.9%, respectively.
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http://dx.doi.org/10.1080/23802359.2019.1694851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720957PMC
December 2019

Characterization of the complete chloroplast genome sequence of (Balsaminaceae): an endemic species from China.

Mitochondrial DNA B Resour 2019 Nov 18;4(2):4073-4074. Epub 2019 Nov 18.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, PR China.

This study was the first report about complete chloroplast genome of (Balsaminaceae). The plastome of was found to possess a total length 152,487 bp with the typical quadripartite structure of angiosperms, containing two inverted repeats (IRs) of 25,684 bp separated by a large single-copy (LSC) region and a small single-copy (SSC) region of 83,290 and 17,829 bp, respectively. The plastid genome of contains 113 genes, including 79 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. The overall GC content of plastid genome is 37.0% and the corresponding values in LSC, SSC, and IR regions are 34.9%, 29.8%, and 43.1%, respectively.
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http://dx.doi.org/10.1080/23802359.2019.1689862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707684PMC
November 2019
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