Publications by authors named "Bing Yan"

768 Publications

Guideline for the evaluation of prescription appropriateness.

Ann Transl Med 2021 Aug;9(16):1352

Department of Pharmacy, Beijing Jishuitan Hospital, Beijing, China.

Evaluation of prescriptions is a necessary process of evaluating the appropriateness of clinical drug usage, discovering existing problems, and formulating solutions. There are challenges for professionals within hospital medical departments and for clinicians and pharmacists who have clinical questions relating to inappropriate or abnormal prescriptions as identified by the electronic evaluation system of prescription. Medications are usually used correctly according to the drug instructions or guidelines. At present, there are no relevant domestic or international guidelines, or principles or standards for identifying inappropriate or abnormal prescriptions. To develop the guideline for evaluation of prescriptions appropriateness in clinical practice, the Pharmaceutical Affairs Commission of the Chinese Hospital Association formed the guideline working group consisting of multidisciplinary experts. The guideline working group summarized clinical questions in the evaluation of prescriptions, searched for supporting evidence, and reached a consensus for recommendations. The guideline contains 6 recommendations for evaluating prescription appropriateness, and the general principle of these recommendations is that clinicians should provide drug instructions, guidelines, or moderate evidence supporting the prescription, and the evaluators will then judge the prescription to be either appropriate or irrational. The recommendations resolve common clinical questions, using supporting examples, explanations and a flow chart. The evaluation of prescription appropriateness could be made more systematic and transparent based on this guideline's conclusions.
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http://dx.doi.org/10.21037/atm-20-7502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422117PMC
August 2021

The leading role of adsorbed lead in PM-induced hippocampal neuronal apoptosis and synaptic damage.

J Hazard Mater 2021 08 20;416:125867. Epub 2021 Apr 20.

Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China; School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China. Electronic address:

Neurodegenerative diseases may be caused by air pollution, such as PM. However, particles still need to be elucidated the mechanism of synergistic neurotoxicity induced by pollutant-loading PM. In this study, we used a reductionist approach to study leading role of lead (Pb) in PM-induced hippocampal neuronal apoptosis and synaptic damage both in vivo and in vitro. Pb in PM caused neurotoxicity: 1) by increasing ROS levels and thus causing apoptosis in neuronal cells and 2) by decreasing the expression of PSD95 via interfering with the calcium signaling pathway through cAMP/CREB/pCREB/BDNF/PSD95 pathway and reducing the synapse length by 50%. This study clarifies a key factor in PM-induced neurotoxicity and provides the experimental basis for reducing PM-induced neurotoxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125867DOI Listing
August 2021

Electrostatic attraction of cationic pollutants by microplastics reduces their joint cytotoxicity.

Chemosphere 2021 Nov 8;282:131121. Epub 2021 Jun 8.

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Institute of Environmental Research at Greater Bay, Guangzhou University, Guangzhou, Guangdong, 510006, China; School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, 266237, China. Electronic address:

Microplastic (MP) pollution causes global concerns regarding the consequential impacts on human health. In particular, MPs may act as vectors for various contaminants to induce adverse effects in human. In this work, the joint cytotoxicity of two different MPs co-exposed with diverse ionic pollutants was investigated in two cell lines from human digestive system: human gastric epithelium (GES-1) and colorectal mucosa (FHC) cell lines. The results indicated that the cytotoxicity of cationic pollutants was alleviated by MPs more significantly than that of anionic pollutants in both culture medium and river water. The electrostatic attraction between the negatively charged MPs and cations was a key factor in determining the ultimate joint toxicity. Our findings indicate that the joint cytotoxicity of MP-pollutant mixtures may be proactively reduced by modulating the surface charge of MPs.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131121DOI Listing
November 2021

Regulator of G-protein signaling 14 protects the liver from ischemia reperfusion injury by suppressing TAK1 activation.

Hepatology 2021 Aug 28. Epub 2021 Aug 28.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, 450052, China.

Background & Aims: Hepatic ischemia-reperfusion injury (IRI) is a common complication of hepatectomy and liver transplantation. However, the mechanisms underlying hepatic IRI have not been fully elucidated. The regulator of G-protein signaling 14 (RGS14) is a multifunctional scaffolding protein that integrates G-protein and mitogen-activated protein kinase (MAPK) signaling pathways. However, the role of RGS14 in hepatic IRI remains unclear.

Approach & Results: We found that RGS14 expression increased in mice subjected to hepatic IR surgery and during hypoxia reoxygenation in hepatocytes. We constructed global RGS14 knockout (RGS14-KO) and hepatocyte-specific RGS14 transgenic (RGS14-TG) mice to establish 70% hepatic IRI models. Histological H&E staining, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), expression of inflammatory factors, and apoptosis were used to assess liver damage and function in these models. We found that RGS14 deficiency significantly aggravated IR-induced liver injury and activated hepatic inflammatory responses and apoptosis in vivo and in vitro. Conversely, RGS14 overexpression exerted the opposite effect of the RGS14-deficient models. Phosphorylation of transforming growth factor-β-activated kinase 1(TAK1) and its downstream effectors JNK and p38 increased in the liver tissues of RGS14-KO mice, but was repressed in those of RGS14-TG mice. Furthermore, inhibition of TAK1 phosphorylation rescued the effect of RGS14 deficiency on JNK and p38 activation, thus blocking the inflammatory responses and apoptosis.

Conclusions: RGS14 plays a protective role in hepatic IR by inhibiting the activation of the TAK1-JNK/p38 signaling pathway. This may be a potential therapeutic strategy for reducing incidences of hepatic IRI in the future.
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http://dx.doi.org/10.1002/hep.32133DOI Listing
August 2021

Meta-Analysis of the Efficacy of Laparoscopic Pyeloplasty for Ureteropelvic Junction Obstruction via Retroperitoneal and Transperitoneal Approaches.

Front Pediatr 2021 29;9:707266. Epub 2021 Jul 29.

Kunming Children's Hospital, Kunming, China.

This study aimed to evaluate the clinical efficacy of laparoscopic pyeloplasty (LP) for ureteropelvic junction obstruction (UPJO) via retroperitoneal and transperitoneal approaches. A systematic literature search on keywords was undertaken using PubMed, Cochrane Library, Embase, China Nation Knowledge (CNKI), and Wanfang. The eligible literature was screened according to inclusion and exclusion criteria. Meta-analysis was performed by using RevMan 5.0 software. According to the inclusion and exclusion criteria, 12 studies were identified with a total of 777 patients. Four hundred eight patients were treated with retroperitoneal laparoscopic pyeloplasty (RLP), and 368 patients were treated with transperitoneal laparoscopic pyeloplasty (TLP). The meta-analysis results showed that the two approaches were similar in terms of presence of postoperative hospital stay, postoperative complication, the rate of conversion, and recurrence ( > 0.05). The operative time in the TLP group was significantly shorter than the RLP group (MD = 16.6; 95% CI, 3.40-29.80; = 0.01). The duration of drainage was significantly shorter (MD = -1.06; 95% CI, -1.92 to -0.19; = 0.02), and the score of postoperative visual analog score (VAS) was significantly lower in the RLP group than in the TLP group (MD = -0.52; 95% CI, -0.96 to -0.08; = 0.02). Both approaches have good success rates and low postoperative complication rates. RLP provides a shorter duration of drainage and lower VAS score, but it takes more operative time than TLP.
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http://dx.doi.org/10.3389/fped.2021.707266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357990PMC
July 2021

Triclosan detoxification through dechlorination and oxidation via microbial Pd-NPs under aerobic conditions.

Chemosphere 2021 Aug 10;286(Pt 3):131836. Epub 2021 Aug 10.

Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, Guangdong, 510405, PR China. Electronic address:

The present study focuses on the successful preparation of microbial palladium nanoparticles (Pd-NPs). The even distribution of Pd in the periplasmic space of B. megaterium Y-4 cells is characterized using a transmission electronic microscopy (TEM) and scanning electron microscope (SEM). X-Ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) revealed the domination of Pd (0) in Pd-NPs. The microbial Pd-NPs were selected to detoxify triclosan (TCS). Liquid chromatography-mass spectrometry (LC-MS) was used to analyze the intermediate products of dechlorination and oxidization. Free radicals quenching and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) capturing experiments confirmed the crucial contribution of atomic H• and O to TCS degradation. Besides, TCS degradation by microbial Pd-NPs could alleviate the cytotoxicity of TCS polluted water. Meanwhile, great circulating utilization of microbial Pd-NPs was obtained in degrading TCS. Corresponding findings in the present study could provide new insight into the role of microbial Pd-NPs in detoxifying pollutants.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131836DOI Listing
August 2021

Axon morphogenesis and maintenance require an evolutionary conserved safeguard function of Wnk kinases antagonizing Sarm and Axed.

Neuron 2021 Sep 11;109(18):2864-2883.e8. Epub 2021 Aug 11.

Life and Medical Sciences Institute (LIMES), Bonn, Germany; VIB Center for Brain & Disease Research, Leuven, Belgium; Department of Neurosciences, KU Leuven, Leuven, Belgium. Electronic address:

The molecular and cellular mechanisms underlying complex axon morphogenesis are still poorly understood. We report a novel, evolutionary conserved function for the Drosophila Wnk kinase (dWnk) and its mammalian orthologs, WNK1 and 2, in axon branching. We uncover that dWnk, together with the neuroprotective factor Nmnat, antagonizes the axon-destabilizing factors D-Sarm and Axundead (Axed) during axon branch growth, revealing a developmental function for these proteins. Overexpression of D-Sarm or Axed results in axon branching defects, which can be blocked by overexpression of dWnk or Nmnat. Surprisingly, Wnk kinases are also required for axon maintenance of adult Drosophila and mouse cortical pyramidal neurons. Requirement of Wnk for axon maintenance is independent of its developmental function. Inactivation of dWnk or mouse Wnk1/2 in mature neurons leads to axon degeneration in the adult brain. Therefore, Wnk kinases are novel signaling components that provide a safeguard function in both developing and adult axons.
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http://dx.doi.org/10.1016/j.neuron.2021.07.006DOI Listing
September 2021

[Mild and moderate postpartum depression treated with acupuncture of a real world study].

Zhongguo Zhen Jiu 2021 Aug;41(8):877-82

Fourth Clinical Medical School of Guangzhou University of CM, Shenzhen 518033, Guangdong Province, China.

Objective: To observe the clinical therapeutic effect on mild and moderate postpartum depression treated with acupuncture of (regulating the conception vessel and unblocking the governor vessel) on the base of real world.

Methods: A total of 116 patients with mild and moderate postpartum depression were divided into an acupuncture group (103 cases) and a non-acupuncture group (13 cases) according to treatment regimen provided. In the acupuncture group, acupuncture of was applied to Baihui (GV 20), Yintang (GV 29), Zhongwan (CV 12), Qihai (CV 6), Guanyuan (CV 4), Neiguan (PC 6), Shenmen (HT 7), Hegu (LI 4), Zusanli (ST 36), Sanyinjiao (SP 6) and Taichong (LR 3). Needles were retained for 30 min each time, the treatment was given once every other day, 3 times a week. In the non-acupuncture group, psychotherapy was provided, once daily. The duration of treatment in the two groups was 8 weeks. According to the treatment times of acupuncture, the acupuncture group was subdivided into an acupuncture A group (60 cases with total treatments ≥ 6 times) and an acupuncture B group (43 cases with total treatments<6 times). Using propensity score matching method, the patients of the acupuncture A and B groups were matched each other. Finally, 31 pairs of cases were matched successfully. Before treatment, at 1st, 2nd, 4th and 8th weeks of treatment, as well as at 3-month follow-up, the scores of Hamilton depression scale (HAMD) were compared in patients among the three groups. Using Logistic regression, the impact of acupuncture frequencies on the therapeutic effect was analyzed and the clinical therapeutic effect was assessed.

Results: The total effective rate of the acupuncture A group was 100.0% (31/31), better than 76.9% (10/13) in the non-acupuncture group and 58.1% in the acupuncture B group (18/31) (<0.05). HAMD score at each time point after treatment was lower than that before treatment in the patients of each group (<0.05). But HAMD score at each time point after treatment in either the acupuncture A group or the acupuncture B group was lower than that in the non-acupuncture group separately (<0.05), HAMD scores in the acupuncture A group at the 4th and 8th weeks of treatment and at follow-up were lower than those in the acupuncture B group (<0.05). Logistic regression analysis showed that the total times of acupuncture treatment and the persistent days of treatment had a certain relation to therapeutic effect (<0.05).

Conclusion: Acupuncture of effectively improves in mild and moderate postpartum depression and its therapeutic effect is closely related to treatment course.
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http://dx.doi.org/10.13703/j.0255-2930.20210201-k0004DOI Listing
August 2021

IGF2-AS knockdown inhibits glycolysis and accelerates apoptosis of gastric cancer cells through targeting miR-195/CREB1 axis.

Biomed Pharmacother 2020 Oct 24;130:110600. Epub 2020 Aug 24.

Department of Gastroenterology, Huaihe Hospital of Henan University, Kaifeng, Henan, China.

Dysregulation of long non-coding RNA (lncRNA) insulin growth factor 2 antisense (IGF2-AS) is being found to have relevance to tumorigenesis, including gastric cancer (GC). The purpose of this study was to further explore the detailed role and molecular mechanism of IGF2-AS in GC progression. The expression levels of IGF2-AS, miR-195 and cAMP responsive element binding protein 1 (CREB1) mRNA were assessed by qRT-PCR. Glucose consumption and lactate production were determined using a corresponding Commercial Assay Kit. Hexokinase 2 (HK2) and CREB1 protein levels were detected using western blot. Cell apoptosis was determined by flow cytometry. The targeted interaction between miR-195 and IGF2-AS or CREB1 was validated using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Our data revealed that IGF2-AS was upregulated in GC tissues and predicted poor prognosis. IGF2-AS knockdown hampered glycolysis and accelerated apoptosis of GC cells. Moreover, IGF2-AS acted as a sponge of miR-195 and CREB1 was a direct target of miR-195. MiR-195 mediated the regulatory effect of IGF2-AS knockdown on GC cell glycolysis and apoptosis. MiR-195 exerted its regulatory effect on GC cell glycolysis and apoptosis by CREB1. Furthermore, IGF2-AS regulated CREB1 expression via sponging miR-195. In conclusion, our study suggested that IGF2-AS knockdown suppressed glycolysis and facilitated apoptosis in GC cells at least partly through sponging miR-195 and modulating CREB1 expression, highlighting a novel therapeutic strategy for GC treatment.
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http://dx.doi.org/10.1016/j.biopha.2020.110600DOI Listing
October 2020

[Diversity and PICRUSt2-based Predicted Functional Analysis of Bacterial Communities During the Start-up of ANAMMOX].

Huan Jing Ke Xue 2021 Aug;42(8):3875-3885

Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330096, China.

Bacterial communities are vital for efficient nitrogen removal in an anaerobic ammonium oxidation (ANAMMOX) system. However, the diversity and functional characteristics of a bacterial community during the start-up of ANAMMOX has not been reported. In this study, an up-flow anaerobic sludge bed reactor was used to start-up the ANAMMOX system, and 16S rRNA high-throughput gene sequencing, combined with PICRUSt2-based functional prediction analysis, was used to investigate the dynamic changes in diversity and function of the bacterial community at different times (d0, d30, d60, and d90) during the start-up. The results showed that 48 phyla, 111 classes, 269 orders, 457 families, 840 genera, and 1497 species were present during the start-up of ANAMMOX. and ia were the main detected ANAMMOX bacteria, and their relative abundance was significantly different at different times during the start-up of ANAMMOX (<0.05). During the start-up, the alpha diversity indices of the bacterial community were significantly decreased (<0.05), and the structure of the bacterial community exhibited significant spatial differentiation (=0.846, <0.01). Functional prediction analysis with PICRUSt2 revealed that the bacterial community was active in organic systems and metabolism at hierarchy level 1, implying abundant functional diversity. Further, the abundance of functional genes was significantly different at hierarchy level 2, during the start-up of ANAMMOX. Forty-nine functional genes involving metabolic nitrogen were detected. The abundance of functional genes, involved in nitrification, denitrification, ANAMMOX, and nitrate and nitrite assimilatory/dissimilatory reduction, changed significantly during the start-up of ANAMMOX.
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http://dx.doi.org/10.13227/j.hjkx.202010232DOI Listing
August 2021

Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis .

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Urology Surgery, Kunming Children's Hospital, Kunming, Yunnan 650228, P.R. China.

Wilms' tumor (WT) is a major type of kidney cancer in children; however, the therapeutic measures for control of tumor metastasis, recurrence and death for this type of cancer remain unsatisfactory. The present study aimed to verify the expression of T‑cell factor 3 (TCF3) in WT, and to explore its role in regulating the viability, migration and apoptosis of kidney tumor cells. Tumor tissues were collected from 10 patients with WT, and adjacent tissues were collected as normal controls. The expression levels of TCF3 were detected in WT tissues and adjacent tissues by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting and immunohistochemistry. In addition, TCF3 expression was silenced in G401 kidney tumor cells via small interfering RNA transfection. Cell viability, cell cycle progression and cell apoptosis were assessed using the MTT assay and flow cytometry; the migration and invasion of kidney tumor cells were examined using Transwell and wound‑healing assays; and the expression levels of Wnt signaling pathway‑related genes (Wnt1, β‑catenin and c‑myc) were detected by RT‑qPCR and western blotting. The results revealed that the expression levels of TCF3 were high in WT tissues from patients. Silencing TCF3 expression in G401 kidney tumor cells significantly inhibited cell viability and migration, and promoted cell apoptosis. Moreover, silencing TCF3 expression in G401 cells inhibited the expression levels of Wnt signaling pathway‑related genes. Overall, these data indicated that TCF3 may be involved in WT development through regulation of Wnt signaling pathways. The findings of the present study provide a novel potential marker for the treatment and prognostic evaluation of WT.
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http://dx.doi.org/10.3892/mmr.2021.12281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299189PMC
September 2021

[email protected] activated peroxymonosulfate system for effectively degrading emerging contaminants: Analysis of the formation and activation mechanism of Fe coordinately unsaturated metal sites.

J Hazard Mater 2021 Oct 29;419:126535. Epub 2021 Jun 29.

Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.

Carbon-encapsulated Fe nanocomposites ([email protected]), obtained by pyrolysis of metal-organic frameworks (MOFs), can activate peroxymonosulfate (PMS) to remove emerging contaminants (ECs). Unfortunately, the current MOFs-derived catalysts always inevitably produce more iron-oxide compounds that unfavorable for PMS activation. In this work, according to the thermogravimetric curve of Fe(II)-MOF-74, to discuss the role of pyrolysis temperature on the structural characteristics of [email protected] The results demonstrated that [email protected] could obtain abundant coordinately unsaturated metal sites and exhibited the best activation performance. Radical-quenching experiments and EPR measurements confirm that the generated sulfate radical (SO˙) and singlet oxygen (O) only degraded approximately 35% of TBBPA. Meanwhile, negatively charged complex intermediates formed by the weak interaction between [email protected] and PMS was proposed as the dominant reactive species, and approximately 65% of TBBPA was degraded. This work optimizes the synthesis strategy and mechanism of [email protected] and provides a methodological reference for the design of Fe-based catalysts.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126535DOI Listing
October 2021

Cytotoxicity Induction by the Oxidative Reactivity of Nanoparticles Revealed by a Combinatorial GNP Library with Diverse Redox Properties.

Molecules 2021 Jun 14;26(12). Epub 2021 Jun 14.

Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay, Ministry of Education, Guangzhou University, Guangzhou 510006, China.

It is crucial to establish relationship between nanoparticle structures (or properties) and nanotoxicity. Previous investigations have shown that a nanoparticle's size, shape, surface and core materials all impact its toxicity. However, the relationship between the redox property of nanoparticles and their toxicity has not been established when all other nanoparticle properties are identical. Here, by synthesizing an 80-membered combinatorial gold nanoparticle (GNP) library with diverse redox properties, we systematically explored this causal relationship. The compelling results revealed that the oxidative reactivity of GNPs, rather than their other physicochemical properties, directly caused cytotoxicity via induction of cellular oxidative stress. Our results show that the redox diversity of nanoparticles is regulated by GNPs modified with redox reactive ligands.
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http://dx.doi.org/10.3390/molecules26123630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231810PMC
June 2021

Plasma Tuning Local Environment of Hexagonal Boron Nitride for Oxidative Dehydrogenation of Propane.

Angew Chem Int Ed Engl 2021 Sep 1;60(36):19691-19695. Epub 2021 Aug 1.

State Key Laboratory of Fine Chemicals, Liaoning Key Laboratory for Catalytic Conversion of Carbon Resources, School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, Liaoning, China.

Hexagonal boron nitride (h-BN) has lately received great attention in the oxidative dehydrogenation (ODH) reaction of propane to propylene for its extraordinary olefin selectivity in contrast to metal oxides. However, high crystallinity of commercial h-BN and elusive cognition of active sites hindered the enhancement of utilization efficiency. Herein, four kinds of plasmas (N , O , H , Ar) were accordingly employed to regulate the local chemical environment of h-BN. N -treated BN exhibited a remarkable activity, i.e., 26.0 % propane conversion with 89.4 % selectivity toward olefins at 520 °C. Spectroscopy demonstrated that "three-boron center" N-defects in the catalyst played a pivotal role in facilitating the conversion of propane. While the sintering effect of the "BO " species in O -treated BN, led to the suppressed catalytic performance (12.4 % conversion at 520 °C).
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http://dx.doi.org/10.1002/anie.202106713DOI Listing
September 2021

Melatonin inhibits gallbladder cancer cell migration and invasion via ERK-mediated induction of epithelial-to-mesenchymal transition.

Oncol Lett 2021 Aug 15;22(2):609. Epub 2021 Jun 15.

ZhengZhou Engineering Laboratory of Organ Transplantation Technique and Application, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

Melatonin is a naturally occurring molecule secreted by the pineal gland that exhibits antitumor properties and prevents the development of human cancer. However, little is known regarding the effects of melatonin on gallbladder cancer (GBC) cells. The present study aimed to investigate the role of melatonin on the prevention of GBC cell invasion. The GBC cell line, GBC-SD, was treated with different concentrations of melatonin for different time periods, and the data indicated that melatonin markedly inhibited the invasion of GBC cells. Following treatment of GBC cells with melatonin, the protein levels of the epithelial marker, E-cadherin, significantly increased, while the expression levels of the mesenchymal markers, N-cadherin, Snail and vimentin, notably decreased. In addition, melatonin inhibited the phosphorylation of ERK1/2. Following treatment of the cells with the ERK activator, tert-Butylhydroquinone, the anti-invasive effects of melatonin were reversed by rescuing epithelial-to-mesenchymal transition in GBC cells. Taken together, these results suggest that melatonin inhibits GBC invasiveness by blocking the ERK signaling pathway. Thus, melatonin may be used as a potential novel cancer therapeutic drug for the treatment of GBC.
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http://dx.doi.org/10.3892/ol.2021.12870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227585PMC
August 2021

Effects of chemical and natural ageing on the release of potentially toxic metal additives in commercial PVC microplastics.

Chemosphere 2021 Nov 18;283:131274. Epub 2021 Jun 18.

Institute of Environmental Research at Greater Bay, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, 510006, China.

Various chemical substances, such as potentially toxic trace metals, are used as plastic additives to improve the performance of polymers and extend the service life of plastic products. However, these added trace metals are likely released from plastic into the environment when the plastic becomes a pollutant, although the process is poorly understood. In this study, chemical ageing of commercial polyvinyl chloride (PVC) microplastics using hydrogen peroxide (HO) and natural ageing of PVC that had been added to an alkaline paddy soil were undertaken to evaluate the potential release of trace metals from PVC. Enhanced release of trace metals from PVC with the increasing HO concentrations was observed, in which the released Pb was 1-2 orders of magnitude higher than other metals (p < 0.01). The released Cr, Ni, Pb, Cu, Zn, Cd and Mn accounted for 87.37%, 79.27%, 22.02%, 20.93%, 17.06%, 15.11%, and 11.02% of their total concentrations (0.28 ± 0.03, 0.08 ± 0.01, 13.67 ± 0.18, 1.07 ± 0.02, 2.20 ± 0.18, 0.05 ± 0.00 and 1.26 ± 0.08 mmol kg) in PVC after ageing with 30% HO, respectively. Compared with the control treatment without PVC addition, the concentrations of CaCl-extractable Cu, Mn, Ni, Pb, and Zn in the soil treated with 5% PVC are significantly increased after incubation for 60 days (p < 0.01). In conclusion, chemical and natural ageing have the potential to lead to the release of Cu, Mn, Ni, Pb, and Zn from the commercial PVC into aquatic and terrestrial environments.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131274DOI Listing
November 2021

The effectiveness of acupoint application of traditional Chinese medicine in treating primary dysmenorrhea: A protocol for meta-analysis and data mining.

Medicine (Baltimore) 2021 Jun;100(24):e26398

Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, Changchun 130117, China.

Background: Primary dysmenorrhea (PD) is a functional disease of the female reproductive system, which has adverse effects on patients' physical and mental health and quality of life. At present, acupoint application of traditional Chinese medicine (TCM) as adjuvant therapy is undergoing clinical trials in different medical centers. However, there is no systematic review or meta-analysis to evaluate the efficacy of acupoint application of TCM in the treatment of PD. There is also a lack of systematic evaluation and analysis of acupoints and herbs.

Methods: All randomized controlled trials related to acupoint catgut embedding therapy on PD will be searched in the following electronic databases: Cochrane Central Registry of controlled trials, PubMed, Wed of Science, EMBASE, Science Net, China Biomedical Literature Database, China Science Journal Database, China National Knowledge Infrastructure and Wan-Fang Database, from inception to May, 2021 were searched without language restrictions. The primary outcomes contain visual analog score, The Cox Menstrual Symptom Scale, while the secondary outcomes consist of adverse events and the recurrence rate. Two reviewers will independently perform data selection, data synthesis, and quality assessment. Data meeting the inclusion criteria will be extracted and analyzed by Revman v.5.3 software. Two reviewers will evaluate the study using the Cochrane collaborative bias risk tool. We will use the scoring method to assess the overall quality of the evidence supporting the main results. We will also use Spass software (version 19.0) for complex network analysis to explore the potential core prescription of acupoint application of traditional Chinese medicine in the treatment of PD.

Results: This study will analyze the clinical effective rate, functional outcomes, quality of life, improvement of clinical symptoms of PD, and effective prescriptions of acupoint application for patients with PD.

Conclusion: Our findings will provide evidence for the effectiveness and potential treatment prescriptions of acupoint application for patients with PD.PROSPERO registration number: CRD 42021244357.
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http://dx.doi.org/10.1097/MD.0000000000026398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213294PMC
June 2021

Superior removal of As(III) and As(V) from water with Mn-doped β-FeOOH nanospindles on carbon foam.

J Hazard Mater 2021 Sep 7;418:126347. Epub 2021 Jun 7.

Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710, USA. Electronic address:

Arsenic pollution of water is one of the severest environmental challenges threatening human health. Iron-based nanomaterials have been demonstrated effective in arsenic removal. However, they generally suffer from low removal efficiency towards highly toxic As(III), loss of active sites owing to agglomeration, and poor reusability. Herein, we report a carbonized melamine foam supported Mn(IV)-doped β-FeOOH nanospindles([email protected] NSp) for tackling the technical hurdles. The designed [email protected] NSp appears as a free-standing monolith through a low-cost and straightforward hydrothermal method. The atomic-scale integration of Mn(IV) into β-FeOOH enables an oxidation-adsorption bifunctionality, where Mn(IV) serves as oxidizer for As(III) and Fe(III) acts as adsorber for As(V). The maximal adsorption capacity for As(V) and As(III) can reach 152 and 107 mg g, respectively. Meanwhile, As in simulated high arsenic groundwater can be decreased to below 10 μg L within 24 h. By simple "filtrating-washing", 85% and 82% of its initial adsorption capacity for As(V) and As(III) can be easily recovered even after 5-cycles reuse. Kinetics and isotherm adsorption study indicate that the arsenic adsorption behavior is mainly through chemical bonding during single-layer adsorbing process. The as-prepared [email protected] offers a scalable, efficient, and recyclable solution for arsenic removal in groundwater and wastewater from mines and industry.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126347DOI Listing
September 2021

Nano-cell and nano-pollutant interactions constitute key elements in nanoparticle-pollutant combined cytotoxicity.

J Hazard Mater 2021 09 2;418:126259. Epub 2021 Jun 2.

School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China; Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Institute of Environmental Research at Greater Bay, Guangzhou University, Guangzhou, Guangdong 510006, China. Electronic address:

As the wide application of carbon nanoparticles (CNPs) and zinc oxide nanoparticles (ZnONPs), as well as ubiquitous chromium (Cr(VI)) pollution in environment, the chance of human exposure to CNPs/ZnONPs and their Cr(VI) adducts is enhanced. We therefore investigated the impacts of nano-cell and nano-Cr(VI) interactions on nanoparticle-Cr(VI) combined cytotoxicity in human lung epithelial (A549) cells. Our results showed that nano-cell and nano-pollutant interactions were the key elements in NP-pollutant combined cytotoxicity, as determined by cell death, oxidative stress and mitochondrial dysfunction. A strong adsorption of Cr(VI) on CNPs and reduction of Cr(VI) to Cr(III) were confirmed, resulting in the reduced cytotoxicity of CNP-Cr(VI) adducts. In contrast, ZnONPs caused the destruction of cell membranes so that more ZnONP-Cr(VI) adducts could enter the cells. Meantime, more Cr contents could be released from ZnONP-Cr(VI) adducts once entering cells and locating in lysosomes than that from CNP-Cr(VI) adducts. These two reasons together caused the enhanced cytotoxicity of ZnONP-Cr(VI) adducts. These findings indicate that the in-depth investigations on the interaction mechanisms are crucial to comprehensively understand the combined cytotoxicity of different NPs and pollutants.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126259DOI Listing
September 2021

A Stable Cd(II)-Based Metal-Organic Framework: Synthesis, Structure, and Its Eu Functionalization for Ratiometric Sensing on the Biomarker 2-(2-Methoxyethoxy) Acetic Acid.

Inorg Chem 2021 Jun 9;60(12):8613-8620. Epub 2021 Jun 9.

School of Chemical Science and Engineering, Tongji University, Siping Road 1239, Shanghai 200092, China.

A novel two-dimensional Cd-based metal-organic framework (MOF), [Cd(pddb)HO] (), has been hydrothermally synthesized using the V-shaped ligand 4,4'-(pyridine-2,6-diyl)-dibenzoic acid (Hpddb) and structurally characterized. The framework exhibits fascinating one-dimensional in-plane channels functionalized with active pyridine-N sites. The as-synthesized exhibits excellent water and chemical stability. Furthermore, a simple and nondestructive coordinated postsynthetic modification method has been applied to to obtain a class of MOF hybrids functionalized by lanthanide ions. More interestingly, [email protected] can act as a dual-emissive ratiometric fluorescent probe for 2-(2-methoxyethoxy) acetic acid (MEAA), a metabolite of 2-(2-methoxyethoxy) ethanol, which could result in DNA damage and teratogenic and developmental toxicity. During the sensing process, the fluorescence sensor exhibits notable water tolerance, reusability, and a low detection limit (8.5 μg mL). In addition, the chemical substances in human urine and serum do not interfere with the fluorescence quenching process, which makes it possible for the fluorescent probe to be applied in the detection of MEAA in human urine and serum systems. The possible sensing mechanism is also studied and discussed in detail.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00589DOI Listing
June 2021

A new strategy to fabricate multifunctional luminescent MOFs, extending their application range from pH sensing to amino acid information coding.

J Colloid Interface Sci 2021 Nov 26;601:427-436. Epub 2021 May 26.

School of Chemical Science and Engineering, Tongji University, Siping Road 1239, Shanghai 200092, China. Electronic address:

Herein, we propose a new strategy for designing new types of wide range pH-sensitive metal-organic frameworks (MOFs) with double luminescent centers on UiO-66-2OH. The UiO-66-2OH has a ligands-based emission at 530 nm. To introduce another luminescent center, PMA (1,2,4,5-benzenetetracarboxylic acid), as the functional site, is used to substitute the initial ligand, BDC-2OH (2,5-dihydroxyterephthalic acid), of UiO-66-2OH. Eu ions, another luminescent center at 613 nm, are coordinated to the free carboxyl group on PMA. Finally, TTA (2-Thenoyltrifluoroacetone) is coordinated with Eu ions to balance the emission at 613 nm (Eu ions) and 530 nm (BDC-2OH). For the sake of both strong emissions, we explored the loading levels of PMA. The optimized structure is Eu(TTA)@MUM5 ("MUM" is the abbreviation of "Mixed ligand UiO-66-2OH MOFs" and "5" represents the molar percentage of PMA is 50%), which exhibits strong emission at 530 nm (alkaline solution) and 613 nm (neutral solution). Remarkably, the synthesized material has an exponential relationship (R = 0.9973) over the pH range of 1.87 to 9.65 and a linear relationship (R = 0.9987) when pH = 11.01-13.35. Further experiments have proved that Eu(TTA)@MUM5 could distinguish different amino acids. Based on that, we build an information transferring circle with two coding modes on Eu(TTA)@MUM5 using aseptic acid and arginine as coding factors.
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http://dx.doi.org/10.1016/j.jcis.2021.05.136DOI Listing
November 2021

Circular RNA 0102049 suppresses the progression of osteosarcoma through modulating miR-520g-3p/PLK2 axis.

Bioengineered 2021 12;12(1):2022-2032

Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou, China.

Circular RNAs (circRNAs) are a type of non-coding RNAs generated from back splicing to enhance or inhibit the progression of multiple human cancers including osteosarcoma (OS). Although circ_0102049 has been found to be highly expressed in OS cell lines, the role and specific mechanism of circ_0102049 in OS remains unclear. Here, we found that silence of circ_0102049 could significantly exacerbate the tumorigenesis of OS through sponging microRNA-520g-3p. Polo-like kinase 2 (PLK2) was predicted to be a target of miR-520g-3p, and luciferase reporter assay revealed that overexpression of miR-520g-3p dramatically suppressed the expression of PLK2, whereas miR-520g-3p inhibitor promoted the PLK2 expression. Moreover, the silence of circ_0102049 could markedly promote the proliferation, invasion, migration and cell-cycle promotion while inhibiting the apoptosis of OS cell line MG63 cells through regulating miR-520g-3p/PLK2 axis. Taken together, the present study indicated that circ_0102049 suppressed the progression of osteosarcoma via modulating miR-520g-3p/PLK2/TAp73 axis, providing a potential therapeutic target for OS.
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http://dx.doi.org/10.1080/21655979.2021.1923259DOI Listing
December 2021

Crystal structure of parallel G-quadruplex formed by the two-repeat ALS- and FTD-related GGGGCC sequence.

Nucleic Acids Res 2021 06;49(10):5881-5890

Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, 00000, China.

The hexanucleotide repeat expansion, GGGGCC (G4C2), within the first intron of the C9orf72 gene is known to be the most common genetic cause of both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The G4C2 repeat expansions, either DNA or RNA, are able to form G-quadruplexes which induce toxicity leading to ALS/FTD. Herein, we report a novel crystal structure of d(G4C2)2 that self-associates to form an eight-layer parallel tetrameric G-quadruplex. Two d(G4C2)2 associate together as a parallel dimeric G-quadruplex which folds into a tetramer via 5'-to-5' arrangements. Each dimer consists of four G-tetrads connected by two CC propeller loops. Especially, the 3'-end cytosines protrude out and form C·C+•C·C+/ C·C•C·C+ quadruple base pair or C•C·C+ triple base pair stacking on the dimeric block. Our work sheds light on the G-quadruplexes adopted by d(G4C2) and yields the invaluable structural details for the development of small molecules to tackle neurodegenerative diseases, ALS and FTD.
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http://dx.doi.org/10.1093/nar/gkab302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191786PMC
June 2021

Multinuclear Lanthanide-Implanted Tetrameric Dawson-Type Phosphotungstates with Switchable Luminescence Behaviors Induced by Fast Photochromism.

Inorg Chem 2021 Jun 20;60(11):8164-8172. Epub 2021 May 20.

Henan Key Laboratory of Polyoxometalate Chemistry, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, China.

A series of benzoate-decorated lanthanide (Ln)-containing tetrameric Dawson-type phosphotungstates [N(CH)]H[{(PWO)Ln(HO)Ln(CHCOO)(HO)]}{[(PWO)Ln(HO)}]Cl·98HO [Ln = Sm (), Eu (), and Gd ()] were made using a facile one-step assembly strategy and characterized by several techniques. Notably, the Ln-containing tetrameric Dawson-type polyoxoanions [{(PWO)Ln(HO)Ln(CHCOO)(HO)]}{[(PWO)Ln(HO)}] are all established by four monolacunary Dawson-type [PWO] segments, encapsulating a Ln ion with two benzoates coordinating to the Ln ions. - exhibit reversible photochromism, which can change from intrinsic white to blue for 6 min upon UV irradiation, and their colors gradually recover for 30 h in the dark. The solid-state photoluminescence spectra of and display characteristic emissions of Ln components based on 4f-4f transitions. Time-resolved emission spectra of and were also measured to authenticate the energy transfer from the phosphotungstate and organic chromophores to Eu. In particular, shows an effectively switchable luminescence behavior induced by its fast photochromism.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00798DOI Listing
June 2021

Safety and efficacy of anti-PD-1 inhibitors in Chinese patients with advanced lung cancer and hepatitis B virus infection: a retrospective single-center study.

Transl Lung Cancer Res 2021 Apr;10(4):1819-1828

Department of Respiratory Diseases, The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou, China.

Background: Programmed death protein (ligand) 1 [PD-(L)1] inhibitors have provided new therapeutic options for advanced lung cancer. However, patients with hepatitis B virus (HBV) infection have been traditionally excluded from most registered trials of this form of treatment.

Methods: We performed a retrospective analysis of patients with HBV and advanced lung cancer who received anti-PD-1 immunotherapy from September 2018 to May 2020 in our department. Treatment-related hepatotoxicity was evaluated and recorded. Overall response rate and progression free survival were also assessed in the patients using iRECIST.

Results: Seventeen patients were evaluated in this analysis. Of these, six (35.3%) experienced hepatic transaminase elevation during immunotherapy. Three of these patients developed Grade 3 hepatic immune-related adverse events and received systemic corticosteroids, following which aminotransferase levels recovered to normal in all patients and no adverse events were observed in subsequent treatment. No patient experienced HBV reactivation or flare. One patient developed active pulmonary tuberculosis (TB). Other adverse events were mild, well tolerated and short term. The objective response rate (ORR) of the cohort was 62.5%, and the median progression-free survival (PFS) was 3 months.

Conclusions: Lung cancer patients can be treated safely with anti-PD-1 inhibitors in the context of HBV infection. Close monitoring for hepatotoxicity and prophylactic antiviral therapy is advised. Further studies on the use of anti-PD-1 inhibitors in HBV-infected patients are needed.
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http://dx.doi.org/10.21037/tlcr-21-79DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107742PMC
April 2021

Linear group 13 E[triple bond, length as m-dash]E triple bonds.

Phys Chem Chem Phys 2021 May;23(20):11611-11615

Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, China.

The unexpected linear group 13 E[triple bond, length as m-dash]E triple bonds were herein uncovered with the D3h-symmetry E2M5+ (M = Li, Na, and K) clusters, where the linear M-E[triple bond, length as m-dash]E-M form is perfectly surrounded by M3 motifs. The increasing nonbonded electron density of the heavier main-group elements is the key issue for the trans-bent geometry, and yet it is strongly suppressed in E2M5+, creating two degenerate π bonds and one multi-center σ bond.
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http://dx.doi.org/10.1039/d1cp01035bDOI Listing
May 2021

Eu functionalized robust membranes based on the post-synthetic copolymerization of a metal-organic framework and ethyl methacrylate.

Dalton Trans 2021 Jun;50(22):7597-7603

School of Chemical Science and Engineering, Tongji University, Shanghai 200092, P. R. China.

Metal-organic frameworks (MOFs) are recognized as a class of promising crystalline materials. However, their subsequent processing and shaping still remain a challenge, and one emerging strategy is to hybridize MOFs with flexible polymers. Herein, by utilizing a simple and cost-effective post-synthetic polymerization method, under mild conditions, MOF particles with olefin bonds are covalently linked to polymer chains. Moreover, photoactive europium ions are also introduced into this system during the polymerization process. Importantly, the resulting MOF-based membrane ([email protected]) is uniform, showing great structural and fluorescence stability against strict conditions (aqueous solutions with pH 0.98-13.11). Besides, given its good luminescence properties, the membrane is employed for the identification of common volatile organic compounds and a selective response to toluene was achieved. This work accelerates the practical applications of MOF-based membranes and enriches the methods for MOF modification.
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http://dx.doi.org/10.1039/d1dt01037aDOI Listing
June 2021

Harmful algal blooms and their eco-environmental indication.

Chemosphere 2021 Jul 15;274:129912. Epub 2021 Feb 15.

Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, China. Electronic address:

Harmful algal blooms (HABs) in freshwater lakes and oceans date back to as early as the 19th century, which can cause the death of aquatic and terrestrial organisms. However, it was not until the end of the 20th century that researchers had started to pay attention to the hazards and causes of HABs. In this study, we analyzed 5720 published literatures on HABs studies in the past 30 years. Our review presents the emerging trends in the past 30 years on HABs studies, the environmental and human health risks, prevention and control strategies and future developments. Therefore, this review provides a global perspective of HABs and calls for immediate responses.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129912DOI Listing
July 2021

Toxic effects of acute exposure to polystyrene microplastics and nanoplastics on the model insect, silkworm Bombyx mori.

Environ Pollut 2021 Sep 30;285:117255. Epub 2021 Apr 30.

Max Planck Partner Group, Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China; Key Laboratory for Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, China. Electronic address:

Microplastics and nanoplastics (MPs and NPs, respectively) are major contaminants of environmental concern due to their potentially detrimental effects on aquatic and terrestrial ecosystems. However, little is known about their potential toxicity in terrestrial organisms. Here, we used the model insect silkworm (Bombyx mori) to evaluate the potential hazardous effects of acute exposure (72 h) to polystyrene (PS) MPs and NPs at physiological, molecular, and biochemical levels as well as their impact on pathogen infection. Our results revealed no significant changes in larval body mass or survival. Nevertheless, exposure led to significant alterations in the expression of immunity-related genes (Cecropin A, Lysozyme, SOD, and GST) and antioxidant-mediated protective response (SOD, GST, and CAT enzymes) which differed in the PS-MP and PS-NP groups. Interestingly, PS-MPs induced a stronger immune response (higher expressions of Lysozyme, SOD, and GST genes along with increased activities of SOD, GST, and CAT enzymes) while the PS-NP response was more that of an inhibitory nature (decreased SOD activity and expression). As a result, upon infection with the natural pathogen Serratia marcescens Bm1, the PS-MP-exposed individuals survived the infection better whereas, PS-NP-exposed individuals exhibited significantly higher mortality. Thus, we infer that PS-MPs/NPs present ecological toxicity, which is closely related to their size, and that their exposure may render the organisms vulnerable or confer resistance to pathogen infections and ecotoxicants. Given the suitability of silkworm as a model organism, this study may promote its application for further investigation of the mechanism of adverse outcome pathways and in studies on bio-nano interactions.
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http://dx.doi.org/10.1016/j.envpol.2021.117255DOI Listing
September 2021
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