Publications by authors named "Bing Song"

261 Publications

CRISPR/Cas9 mediated β-globin gene knockout in rabbits recapitulates human β-thalassemia.

J Biol Chem 2021 Feb 24:100464. Epub 2021 Feb 24.

Key Lab for Major Obstetric Diseases of Guangdong Province, Experimental Department of Institute of Gynecology and Obstetrics, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China. Electronic address:

β-thalassemia, autosomal recessive blood disorders that reduce the production of hemoglobin, are majorly caused by the point mutation of the HBB gene resulting in reduced or absent β-globin chains of the hemoglobin tetramer. Animal models recapitulating both the phenotype and genotype of human disease are valuable in the exploration of pathophysiology and for in vivo evaluation of novel therapeutic treatments. The docile temperament, short vital cycles and low cost of rabbits make them an attractive animal model. However, β-thalassemia rabbit models are currently unavailable. Here, using CRISPR/Cas9-mediated genome editing, we point mutated the rabbit β-globin gene HBB2 with high efficiency and generated a β-thalassemia rabbit model. Hematological and histological analyses demonstrated that the genotypic mosaic F0 displayed a mild phenotype of anemia, and the heterozygous F1 exhibited typical characteristics of β-thalassemia. Whole blood transcriptome analysis revealed that the gene expression was altered in HBB2-targeted when compared to WT rabbits. And the highly expressed genes in HBB2-targeted rabbits were enriched in lipid and iron metabolism, innate immunity and hematopoietic processes. In conclusion, using CRISPR-mediated HBB2 knockout, we have created a β-thalassemia rabbit model the accurately recapitulates the human disease phenotype. We believe this tool will be valuable in advancing the investigation of pathogenesis and novel therapeutic targets of β-thalassemia and associated complications.
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http://dx.doi.org/10.1016/j.jbc.2021.100464DOI Listing
February 2021

A Compact Model of Ovonic Threshold Switch Combining Thermal Dissipation Effect.

Front Neurosci 2021 9;15:635264. Epub 2021 Feb 9.

College of Electronic Science and Technology, National University of Defense Technology, Changsha, China.

Ovonic threshold switch (OTS) has received great attention in neuromorphic computing due to its support for high-density synapse array as a selector and leaky-integration-firing functions Hodgkin-Huxley neurons. However, there is no simple and complete model for device simulation and integrated circuit design, which hindered application until now. In this work, we developed a compact physical model of OTS based on the Poole-Frenkel effect accompanied by the thermal dissipation effect for the first time. The thermal dissipation effect describes the energy flow between the device and the environment so that the model is more practical. Compared with previous experiments, the numerical results fairly fitted the electrical characteristics, demonstrating the model validity. In addition, the relation of the device performance with material and structure was deduced, which can facilitate optimizing the OTS device. The model will be useful for device design and implemented with high speed for simplicity.
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http://dx.doi.org/10.3389/fnins.2021.635264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901986PMC
February 2021

Differences in the Gut Microbiome of Women With and Without Hypoactive Sexual Desire Disorder: Case Control Study.

J Med Internet Res 2021 Feb 25;23(2):e25342. Epub 2021 Feb 25.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Background: The gut microbiome is receiving considerable attention as a potentially modifiable risk factor and therapeutic target for numerous mental and neurological diseases.

Objective: This study aimed to explore and assess the difference in the composition of gut microbes and fecal metabolites between women with hypoactive sexual desire disorder (HSDD) and healthy controls.

Methods: We employed an online recruitment method to enroll "hard-to-reach" HSDD populations. After a stringent diagnostic and exclusion process based on DSM-IV criteria, fecal samples collected from 24 women with HSDD and 22 age-matched, healthy controls underwent microbiome analysis using 16S ribosomal RNA gene sequencing and metabolome analysis using untargeted liquid chromatography-mass spectrometry.

Results: We found a decreased abundance of Ruminococcaceae and increased abundance of Bifidobacterium and Lactobacillus among women with HSDD. Fecal samples from women with HSDD showed significantly altered metabolic signatures compared with healthy controls. The abundance of Bifidobacterium, Lactobacillus, and several fecal metabolites correlated negatively with the sexual desire score, while the number of Ruminococcaceae correlated positively with the sexual desire score in all subjects.

Conclusions: Our analysis of fecal samples from women with HSDD and healthy controls identified significantly different gut microbes and metabolic signatures. These preliminary findings could be useful for developing strategies to adjust the level of human sexual desire by modifying gut microbiota.

Trial Registration: Chinese Clinical Trial Registry ChiCTR1800020321; http://www.chictr.org.cn/showproj.aspx?proj=34267.
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http://dx.doi.org/10.2196/25342DOI Listing
February 2021

Generation of induced pluripotent stem cell GZHMCi005-A from amniotic fluid-derived cells with duplication of chromosome 8p.

Stem Cell Res 2021 Feb 5;52:102226. Epub 2021 Feb 5.

Key Lab for Major Obstetric Diseases of Guangdong Province, Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, China. Electronic address:

Cases in which the duplication of chromosome 8p (dup 8p) is observed are characterized by facial dysmorphism, agenesis/hypoplasia of the corpus callosum, heart defects and severe mental retardation. The frequency of dup 8p cases is higher compared to other chromosomes because of the Non-allelic homologous recombination (NAHR) between two segmental duplication regions (SDs) containing olfactory receptor gene clusters, REPD (repeat-distal) and REPP (repeat-proximal), located in chromosome 8p23.1. Here we generated a human iPSC line from a patient's amniotic fluid cells with a 18 Mb duplication in 8p23.3p22, which will serve as useful tools for studying dup 8p syndrome.
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http://dx.doi.org/10.1016/j.scr.2021.102226DOI Listing
February 2021

Clinical mid-term outcomes of the Chinese-made CL-V bileaflet mechanical heart valve in Chinese patients.

J Thorac Dis 2021 Jan;13(1):10-17

The First School of Clinical Medicine, Lanzhou University, Lanzhou, China.

Background: The CL-V full-carbon bileaflet mechanical heart valve is a novel Chinese-made prosthetic valve. This study evaluated the mid-term outcomes of the CL-V bileaflet mechanical heart valve after implantation in Chinese patients.

Methods: This study retrospectively enrolled a total of 38 consecutive patients who underwent elective mechanical heart valve replacement (MHVR) with two different valve types from April 2004 and May 2010, including 18 patients with the CL-V bileaflet mechanical heart valve (44.4% male, mean age 47.4±6.2 years, mean body weight 64.7±11.9 kg) and 20 patients with the St. Jude mechanical heart valve (45.0% male, mean age 49.7±7.6 years, mean body weight 66.1±11.1 kg). All patients underwent follow-up clinical evaluations in the outpatient department at all-time points.

Results: No complications occurred during the mean 61.3 months follow-up time (range, 47-102 months). The cardiothoracic ratios (52.7%±4.5% 50.1%±4.0%), left atrium diameter (46.5±7.6 44.8±9.3 mm), left ventricular diastolic diameter (47.6±4.9 48.2±8.5 mm) and left ventricular ejection fraction (65.4%±8.7% 64.5%±8.0%) were not significantly different between the two groups (P>0.05). Transthoracic Doppler echocardiography showed that the hemodynamic indexes were not significantly different between the two groups at 1 year and 3 years (P>0.05). Furthermore, no significant differences were found between the two groups in hemocompatibility indexes at both 6 months and 3 years postoperatively (P>0.05).

Conclusions: The mid-term follow-up results of the CL-V bileaflet mechanical heart valve were similar to those of the St. Jude Medical heart valve, which showed stable hemodynamics and good blood compatibility. Chinese-made CL-V bileaflet mechanical heart valves can be a substitute for St. Jude Medical heart valves, and can be widely used in cardiac surgery.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000034158.
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http://dx.doi.org/10.21037/jtd-20-2644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867806PMC
January 2021

Novel compound heterozygous variants in dynein axonemal heavy chain 17 cause asthenoteratospermia with sperm flagellar defects.

J Genet Genomics 2020 Oct 9. Epub 2020 Oct 9.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, Hefei, 230032, China; Ministry of Education Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Hefei, 230032, China; Anhui Province Key Laboratory of Reproductive Health and Genetics, Hefei, 230032, China; Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, 230032, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2020.07.004DOI Listing
October 2020

mixIndependR: a R package for statistical independence testing of loci in database of multi-locus genotypes.

BMC Bioinformatics 2021 Jan 6;22(1):12. Epub 2021 Jan 6.

Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA.

Background: Multi-locus genotype data are widely used in population genetics and disease studies. In evaluating the utility of multi-locus data, the independence of markers is commonly considered in many genomic assessments. Generally, pairwise non-random associations are tested by linkage disequilibrium; however, the dependence of one panel might be triplet, quartet, or other. Therefore, a compatible and user-friendly software is necessary for testing and assessing the global linkage disequilibrium among mixed genetic data.

Results: This study describes a software package for testing the mutual independence of mixed genetic datasets. Mutual independence is defined as no non-random associations among all subsets of the tested panel. The new R package "mixIndependR" calculates basic genetic parameters like allele frequency, genotype frequency, heterozygosity, Hardy-Weinberg equilibrium, and linkage disequilibrium (LD) by mutual independence from population data, regardless of the type of markers, such as simple nucleotide polymorphisms, short tandem repeats, insertions and deletions, and any other genetic markers. A novel method of assessing the dependence of mixed genetic panels is developed in this study and functionally analyzed in the software package. By comparing the observed distribution of two common summary statistics (the number of heterozygous loci [K] and the number of share alleles [X]) with their expected distributions under the assumption of mutual independence, the overall independence is tested.

Conclusion: The package "mixIndependR" is compatible to all categories of genetic markers and detects the overall non-random associations. Compared to pairwise disequilibrium, the approach described herein tends to have higher power, especially when number of markers is large. With this package, more multi-functional or stronger genetic panels can be developed, like mixed panels with different kinds of markers. In population genetics, the package "mixIndependR" makes it possible to discover more about admixture of populations, natural selection, genetic drift, and population demographics, as a more powerful method of detecting LD. Moreover, this new approach can optimize variants selection in disease studies and contribute to panel combination for treatments in multimorbidity. Application of this approach in real data is expected in the future, and this might bring a leap in the field of genetic technology.

Availability: The R package mixIndependR, is available on the Comprehensive R Archive Network (CRAN) at: https://cran.r-project.org/web/packages/mixIndependR/index.html .
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http://dx.doi.org/10.1186/s12859-020-03945-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788837PMC
January 2021

A Fenton-like system of biochar loading Fe-Al layered double hydroxides (FeAl-LDH@BC) / HO for phenol removal.

Chemosphere 2021 Mar 18;266:128992. Epub 2020 Nov 18.

Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing, 210096, China. Electronic address:

FeAl-layered double hydroxide (FeAl-LDH) supported by char was synthesized using the hydrothermal method in order to activate hydrogen peroxide (HO) to degrade phenol. The effects of char type, char synthesis amount, and several important parameters on the degradation were investigated. In addition, the physicochemical properties of FeAl-LDH@BC were revealed by instruments including the transmission electron microscope (TEM), X-ray diffraction (XRD), and Fourier-transform infrared (FT-IR). The results showed that the degradation efficiency of phenol (80 mg/L) by FeAl-LDH@BC was 85.28% at a pH of 3 and HO concentration of 400 mg/L, and exhibited good reusability with a small amount of iron leaching. Electron paramagnetic resonance (EPR) and radical quenching results indicated that ·OH radicals were the main participant during the degradation process, and XRD and FTIR spectra showed that FeAl-LDH was dissolved and rebuilt during the degradation process, and a small amount of iron was leached out resulting in the homogeneous catalysis. Hence, both homogeneous and heterogeneous processes occurred in the phenol oxidation process. Further soil remediation experiments showed that FeAl-LDH@BC could also effectively degrade phenol in soil, although the efficiency was lower than that in solution.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128992DOI Listing
March 2021

Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation.

Aging (Albany NY) 2020 11 26;13(1):525-536. Epub 2020 Nov 26.

Department of Pharmacy, Taikang Xianlin Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Background and aims Advanced glycation end products (AGEs) and receptor of advanced glycation end products (RAGE), are associated with cognition decline. We aim to investigate the effect of liraglutide on cognitive function in diabetic mice. Results Diabetic mice showed decreased cognitive function. Moreover, lower glucagon like peptide-1 (GLP-1) levels in plasma were detected in db/db mice. Additionally, up-regulated RAGE and down-regulated glucagon like peptide-1 (GLP-1R) levels were observed in db/db mice. However, decreased GLP-1R and increased RAGE were reversed by liraglutide. We also found decreased cellular activity in cells with AGEs. Moreover, AGEs up-regulated RAGE in PC12 and HT22 cells. However, liraglutide improved the cell activity damaged by AGEs. Although we did not discover the direct-interaction between RAGE and GLP-1R, elevated RAGE levels induced by AGEs were restored by liraglutide. Conclusion We demonstrated that the cognitive function of diabetic mice was improved by liraglutide via the down-regulation of RAGE. Methods db/db mice and db/m mice were used in this study. Liraglutide was used to remedy diabetic mice. Neurons and RAGE in hippocampus were shown by immunofluorescence. And then, PC12 cells or HT22 cells with AGEs were treated with liraglutide. GLP-1R and RAGE were measured by western blotting.
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http://dx.doi.org/10.18632/aging.202162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835012PMC
November 2020

Effect of different drying temperatures on the rehydration of the fruiting bodies of Yu Muer () and screening of browning inhibitors.

Food Sci Nutr 2020 Nov 27;8(11):6037-6046. Epub 2020 Sep 27.

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education Jilin Agricultural University Changchun China.

In this study, the color of the dry fruiting bodies, fresh weight (FW): dry weight (DW) ratio, amino acids, and total phenolics, which are of nutritional or commercial interest, were compared among different drying temperature treatments. The effect of rehydration methods and color protection reagents on the fruiting body color, polyphenol oxidase (PPO) activity, and browning inhibition rate were evaluated. The results showed that drying with hot air at 65℃ was quickest and resulted in a better color without compromising the FW:DW ratio and rehydration ratio of the fruiting bodies. Furthermore, some reactions that occurred under high temperatures increased the content of protein, amino acids, and total phenolics. Soaking after boiling was the most suitable rehydration method, leading to the lowest PPO activity (39.87 ± 1.35 U/g). All of the four analyzed color protection reagents could significantly inhibit the browning of Yu Muer fruiting bodies under room temperature water rehydration conditions, with a citric acid content of 6 g/L showing the best performance. These results provide technical support for the development of the Yu Muer industry and for promoting the commercial processing of Yu Muer fruiting bodies slices.
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http://dx.doi.org/10.1002/fsn3.1891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684618PMC
November 2020

Generation of induced pluripotent stem cell GZLSL-i001-A derived from urine-derived cells of Hemophilia A patient with Inv22 mutation.

Stem Cell Res 2020 12 19;49:102053. Epub 2020 Oct 19.

Key Lab for Major Obstetric Diseases of Guangdong Province, Experimental Department of Institute of Gynecology and Obstetrics, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China. Electronic address:

Hemophilia A (HA), is a X-linked recessive congenital bleeding disorder, caused by deficiency of the coagulation factorVIII (FVIII) which is encoded by coagulation factor 8 (F8). HA affects 1 of every 5,000 males worldwide. The intron 22 inversion (Inv22) mutation of F8 causes about 45% of severeHA cases.Here, we generated induced pluripotent stem cells (iPSCs) from a HA patient with Inv22 mutation by electroporation of urine-derived cells (UCs) with episomal plasmids under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. This iPSCs line could facilitate future applications of human iPSCs by provide a valuable cell model.
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http://dx.doi.org/10.1016/j.scr.2020.102053DOI Listing
December 2020

A 5-Year Review of Invasive Fungal Infection at an Academic Medical Center.

Front Cell Infect Microbiol 2020 22;10:553648. Epub 2020 Oct 22.

Department of Dermatology, The First Hospital of China Medical University, Shenyang, China.

Invasive fungal infection (IFI) is one of the most common nosocomial infections. However, data on the epidemiology of IFI and susceptibility to antifungal agents in China are quite limited, and in particular, no current data exist on the microbiological, and clinical characteristics of IFI patients in Northeast China. The purpose of this study was to provide a retrospective review of the clinical characteristics, laboratory test results, and risk factor predictions of inpatients diagnosed with IFI. Multivariate regression analysis was used to assess prognostic factors associated with the mortality of these patients. We retrospectively analyzed the results from 509 patients with IFI extracted from the First Hospital of China Medical University from January 2013 to January 2018. Neutrophil numbers, total bilirubin, length of stay in the ICU, renal failure, use of immunosuppressants within the past 30 days, stomach tube placement and septic shock were risk factors for death from IFI. Recent surgery (within 2 weeks) and drainage tube placement did not increase mortality in these IFI patients. Increased serum levels of PCT (AUC 0.601, 95% CI 0.536-0.665, = 0.003) and CRP (AUC 0.578, 95% CI 0.512-0.644, = 0.020) provided effective predictors of 30-day mortality rates. We report for the first time epidemiological data on invasive fungal infections in Northeast China over the past 5 years. Despite the limited available clinical data, these findings will greatly aid clinical health care workers with regard to the identification, prevention, and treatment of IFI in hospitalized patients.
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http://dx.doi.org/10.3389/fcimb.2020.553648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642834PMC
October 2020

Primary cardiac epithelioid haemangioendothelioma.

Eur Heart J 2020 Nov 7. Epub 2020 Nov 7.

Department of Cardiovascular Surgery, Lanzhou University First Affiliated Hospital, Gansu Province, China.

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http://dx.doi.org/10.1093/eurheartj/ehaa814DOI Listing
November 2020

[miR-186-5p down-regulates TLR3 expression to inhibit apoptosis of cardiomyocytes induced by high glucose].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Oct;36(10):877-883

Electrocardial Center, Jinzhou Medical University, Jinzhou 121000, China.

Objective To investigate the expression levels of microRNA-186-5p (miR-186-5p) and Toll-like receptor 3 (TLR3) and their relationships with the apoptosis in high-glucose (HG)-treated AC16 cardiomyocytes. Methods Target Scan7.1 database predicted that miR-186-5p could act directly on TLR3. Diabetic cardiomyopathy model was established in cardiomyocytes stimulated by HG. The expression of miR-186-5p was detected by real-time quantitative PCR and the expression of TLR3 was detected by Western blot analysis. The expression of miR-186-5p or TLR3 was enhanced or reduced by cell transfection. The apoptosis of cardiomyocytes was detected by flow cytometry. The expression of cleaved caspase-3(c-caspase-3) was detected by Western blot analysis, and the interaction between miR-186-5p and TLR3 was analyzed by luciferase activity assay. Results The bioinformatics analysis and luciferase activity assay showed that TLR3 was a direct target gene of miR-186-5p. The expression of miR-186-5p was down-regulated in HG-treated cardiomyocytes, and the over-expression of miR-186-5p reversed HG-induced cardiomyocyte apoptosis and reduced the protein level of c-caspase-3. Down-regulation of TLR3 inhibited HG-induced apoptosis and reduced protein level of c-caspase-3 in cardiomyocytes. Over-expression of TLR3 increased HG-induced cardiomyocyte apoptosis and reversed the effect of miR-186-5p. Conclusion The miR-186-5p can inhibit the apoptosis of cardiomyocytes induced by HG via down-regulating TLR3 expression.
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October 2020

Generation of induced pluripotent stem cell GZHMCi002-A from peripheral blood mononuclear cells with APOB mutation.

Stem Cell Res 2020 12 16;49:102054. Epub 2020 Oct 16.

Key Lab for Major Obstetric Diseases of Guangdong Province, Experimental Department of Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, 510005 Guangzhou, China. Electronic address:

Apolipoprotein (apo) B is a large, amphipathic glycoprotein which plays an important role in human lipoprotein metabolism. The 43-kb APOB gene located on the short arm of human chromosome 2 and consisted of 29 exons, mutations in the APOB gene can give rise to either hypo- or hypercholesterolemia. We used peripheral blood mononuclear cells (PBMCs) from a volunteer carrying the APOB mutation (c.10579C>T, p.Arg3527Trp) located in exon 9 to establish induced pluripotent stem cells (iPSC), which will be an effective means to reveal the key biologically relevant metabolic mechanisms, a powerful tool for medicine selection and related research.
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http://dx.doi.org/10.1016/j.scr.2020.102054DOI Listing
December 2020

Identification of Subtype-Specific Metastasis-Related Genetic Signatures in Sarcoma.

Front Oncol 2020 6;10:544956. Epub 2020 Oct 6.

Department of Dermatology, The First Hospital of China Medical University, Shenyang, China.

Sarcomas are heterogeneous rare malignancies constituting approximately 1% of all solid cancers in adults and including more than 70 histological and molecular subtypes with different pathological and clinical development characteristics. We identified prognostic biomarkers of sarcomas by integrating clinical information and RNA-seq data from TCGA and GEO databases. In addition, results obtained from cell cycle, cell migration, and invasion assays were used to assess the capacity for Tanespimycin to inhibit the proliferation and metastasis of sarcoma. Sarcoma samples ( = 536) were divided into four pathological subtypes including DL (dedifferentiated liposarcoma), LMS (leiomyosarcoma), UPS (undifferentiated pleomorphic sarcomas), and MFS (myxofibrosarcoma). RNA-seq expression profile data from the TCGA dataset were used to analyze differentially expressed genes (DEGs) within metastatic and non-metastatic samples of these four sarcoma pathological subtypes with DEGs defined as metastatic-related signatures (MRS). Prognostic analysis of MRS identified a group of genes significantly associated with prognosis in three pathological subtypes: DL, LMS, and UPS. , and were found to be more likely associated with adverse prognosis. We also identified Tanespimycin as a drug exerting inhibitory effects on metastatic LMS subtype and therefore can serve a potential treatment for this type of sarcoma. These results provide new insights into the pathogenesis, diagnosis, treatment, and prognosis of sarcomas and provide new directions for further study of sarcoma.
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http://dx.doi.org/10.3389/fonc.2020.544956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573283PMC
October 2020

Promoting adsorption of organic pollutants via tailoring surface physicochemical properties of biomass-derived carbon-attapulgite.

Environ Sci Pollut Res Int 2021 Mar 28;28(9):11106-11118. Epub 2020 Oct 28.

Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Jiangsu Province Key Laboratory of Environmental Engineering, Nanjing, 210096, Jiangsu, China.

Biomass-derived carbon-attapulgite adsorbent was developed for organic pollutants removal. All the batch assays were performed to evaluate the effects of organic components, contact time, and initial concentration of organic pollutants on the adsorption performance of the as-prepared adsorbent. The samples were characterized via Brunauer-Emmett-Teller (BET), Fourier transform infrared (FTIR), X-ray diffractometer (XRD), and scanning electron microscopy (SEM). The results demonstrated that the acid-treated carbon-attapulgite adsorbent (H-ATP/BC) showed a large specific surface area (237 m g) and possessed abundant oxygen-containing functional groups and silicon-oxygen bonds (i.e., O-Si-O and O-Si), which provided more active sites and conduced to the adhesive of organic pollutants. Both physical adsorption and chemical adsorption were involved in the adsorption process, and competitive adsorption occurred when two or more target pollutants coexist. Especially, phenol and/or aniline with an aromatic ring were much more likely to adhere to the H-ATP/BC surface than pyridine, and the selectivity order of H-ATP/BC for these pollutants was phenol > aniline > pyridine. From the model fitting, it was observed that the adsorption data could be described well by a pseudo-second-order model and Freundlich isotherms. The theoretical maximum phenol, aniline, and pyridine adsorption capacities of the H-ATP/BC were 14.31 mg g, 15.21 mg g, and 20.74 mg g, respectively. Comparison among the commercial adsorbents price also illustrated that H-ATP/BC could be a promising material for efficient treatment of organic pollutants.Graphical abstract.
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http://dx.doi.org/10.1007/s11356-020-10974-yDOI Listing
March 2021

Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells.

J Dent 2020 Dec 14;103:103501. Epub 2020 Oct 14.

Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China. Electronic address:

Objectives: This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration.

Methods: 0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. "Osteodentine" differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining.

Results: The ELISA results showed that liposomal TGF-β1 achieved a controlled and prolonged release over time. The MTT results demonstrated that the liposomes (100 μg/mL) were not cytotoxic to the cells. Liposomal TGF-β1 up-regulated the expression of "osteodentine" markers, RUNX-2, DMP-1 and DSPP, in hDPSCs after 7 days of treatment and resulted in the accumulation of mineralised nodules.

Conclusion: This study indicated that liposomes are an effective carrier for delivering TGF-β1 over time. Liposomal TGF-β1 promoted dentinogenesis and increased mineralisation in hDPSCs. This highlights the potential of liposomal TGF-β1 for future use in dentine-pulp regeneration.

Clinical Significance: Liposomal TGF-β1 may be used as a synergist for promoting dentine-pulp regeneration of immature permanent teeth or as a pulp capping agent for inducing reparative dentine formation.
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http://dx.doi.org/10.1016/j.jdent.2020.103501DOI Listing
December 2020

Three Case Reports of Intravenous Leiomyomatosis with Intracardiac Extensions.

Thorac Cardiovasc Surg Rep 2020 Jan 18;9(1):e40-e43. Epub 2020 Sep 18.

Department of Cardiovascular Surgery, Lanzhou University First Affiliated Hospital, Lanzhou, China.

 Intravenous leiomyomatosis is a rare disease. Histologically, intravenous leiomyomatosis is a benign tumor, but its biological behavior can be malignant. The development of intracardiac extensions leads to congestive heart failure and occasionally sudden fatalities.  The cases of three patients treated at our university between 2017 and 2018 were studied retrospectively. Intravenous tumors extending into the right heart system were fully removed without perioperative complications or death. Only one tumor recurrence was observed during the followed-up period.  The gold standard for the treatment of intravenous leiomyomatosis with intracardiac extension is complete and successful surgical resection.
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http://dx.doi.org/10.1055/s-0040-1715183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500974PMC
January 2020

Surgical Strategy for Intravenous Cardiac Leiomyomatosis.

Heart Lung Circ 2021 Feb 21;30(2):240-246. Epub 2020 Aug 21.

Department of Cardiovascular Surgery, The First Hospital of Lanzhou University, Gansu Province, China. Electronic address:

Intravenous-cardiac leiomyomatosis (IVCL) is a rare, histologically benign but biologically aggressive tumour. Accurate diagnosis and appropriate treatment choices are important for prognosis. The best surgical approach remains unclear owing to limited evidence. This study aimed to assess surgical strategies for treating IVCL and to propose individualised surgical strategies. We searched PubMed, Web of Science, and the Cochrane Library to identify case reports and case series published in English over the last 10 years. Two (2) reviewers independently screened the literature according to the inclusion and exclusion criteria, and subsequently extracted data. One hundred and ten (110) cases were included. According to our analysis, IV cardiac leiomyomatosis is most common in the fifth decade, and the mean age at detection is 45.71±7.67 years. Most patients had undergone previous hysterectomy/myomectomy, or had a co-existing uterine leiomyoma when admitted. The most common clinical presentations were dyspnoea, palpitation, pelvic mass, and leg oedema. More patients benefited more from one-stage surgery. Seventy-eight per cent (78%) of cases experienced a surgical approach of thoracic and abdominal incisions. Extractions of IVCL were varied. Eighty-seven (87) patients experienced cardiopulmonary bypass and deep hypothermic circulatory arrest was performed on 64.4% of them. Total hysterectomy and bilateral oophorectomy, together with pelvic leiomyoma excision, were done in 76.0% of patients. Overall, surgical strategies for IVCL are varied; the optimal strategy required consideration of multiple factors. Complete resection is recommended for both single- and two-stage operations. Once complete resection is achieved, recurrence is rare.
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http://dx.doi.org/10.1016/j.hlc.2020.07.006DOI Listing
February 2021

Comparison of microbial communities during anaerobic digestion of kitchen waste: Effect of substrate sources and temperatures.

Bioresour Technol 2020 Dec 14;317:124016. Epub 2020 Aug 14.

Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510006, China; Guangzhou Institute of Energy Conversion, CAS Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China. Electronic address:

In this study, batch experiments were conducted to compare the effect of temperature and substrate source on microbial communities in the anaerobic digestion of kitchen waste. The results showed that the microbial communities of anaerobic digestion were not sensitive to varied sources of waste, but shifted with the change in operating temperatures. In the reactors operated at mesophilic conditions, Levilinea, Syntrophomonas, Methanothrix, and Methanosphaerula, etc. were the dominant microbes during the process. While in thermophilic reactors, Levilinea, Ornatilinea, Methanosphaerula and Methanomassiliicoccus, etc. prevailed. Meanwhile, an enrichment in Coprothermobacter, Defluviitoga, Defluviitalea, Tepidimicrobium, Lutispora and Fonticella were observed as the temperature changed from mesophilic to thermophilic, suggesting these genera could be selectively enriched at thermophilic conditions. The results provided fundamental understanding of the microbiology that could support the scale up of food waste anaerobic digestion.
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http://dx.doi.org/10.1016/j.biortech.2020.124016DOI Listing
December 2020

Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer.

Arch Virol 2020 Nov 18;165(11):2689-2693. Epub 2020 Aug 18.

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China.

Here, we describe a novel mycovirus, Auricularia heimuer fusarivirus 1 (AhFV1), isolated from the edible fungus Auricularia heimuer strain CCMJ1296. The virus has a single-stranded positive-sense [+ssRNA] genome of 7,127 nucleotides containing two overlapping open reading frames (ORFs) and a poly(A) tail. The large ORF1 encodes a polyprotein of 1,637 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and DEAD-like helicase superfamily (DEXDc) domains. ORF2 encodes a putative 633-aa protein with unknown function. A BLAST search showed that the nucleotide sequence of the AhFV1 genome is 41.28% identical to that of Sclerotium rolfsii fusarivirus 2 and 40.49% identical to that of Sclerotium rolfsii fusarivirus 1. Phylogenetic analysis based on RdRp and helicase (Hel) sequences indicated that AhFV1 is related to unclassified mycoviruses and other fusariviruses. Our data suggest that AhFV1 should be classified as a member of the newly proposed family "Fusariviridae". This is the second virus and the first full genome sequence of a fusarivirus from A. heimuer.
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http://dx.doi.org/10.1007/s00705-020-04781-6DOI Listing
November 2020

Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice.

Am J Hum Genet 2020 09 12;107(3):514-526. Epub 2020 Aug 12.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), Hefei 230032, China; Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People's Republic of China, Hefei 230032, China. Electronic address:

Multiple morphological abnormalities of the sperm flagella (MMAF) is a severe form of asthenoteratozoospermia. Although recent studies have revealed several MMAF-associated genes and demonstrated MMAF to be a genetically heterogeneous disease, at least one-third of the cases are still not well understood for their etiology. Here, we identified bi-allelic loss-of-function variants in CFAP58 by using whole-exome sequencing in five (5.6%) unrelated individuals from a cohort of 90 MMAF-affected Chinese men. Each of the men harboring bi-allelic CFAP58 variants presented typical MMAF phenotypes. Transmission electron microscopy demonstrated striking flagellar defects with axonemal and mitochondrial sheath malformations. CFAP58 is predominantly expressed in the testis and encodes a cilia- and flagella-associated protein. Immunofluorescence assays showed that CFAP58 localized at the entire flagella of control sperm and predominantly concentrated in the mid-piece. Immunoblotting and immunofluorescence assays showed that the abundances of axoneme ultrastructure markers SPAG6 and SPEF2 and a mitochondrial sheath protein, HSP60, were significantly reduced in the spermatozoa from men harboring bi-allelic CFAP58 variants. We generated Cfap58-knockout mice via CRISPR/Cas9 technology. The male mice were infertile and presented with severe flagellar defects, consistent with the sperm phenotypes in MMAF-affected men. Overall, our findings in humans and mice strongly suggest that CFAP58 plays a vital role in sperm flagellogenesis and demonstrate that bi-allelic loss-of-function variants in CFAP58 can cause axoneme and peri-axoneme malformations leading to male infertility. This study provides crucial insights for understanding and counseling of MMAF-associated asthenoteratozoospermia.
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http://dx.doi.org/10.1016/j.ajhg.2020.07.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477015PMC
September 2020

Mirror-image dextrocardia: why is the apex impulse not on the right?

Authors:
Ye Liu Bing Song

Herz 2020 Aug 6. Epub 2020 Aug 6.

Department of Endocrine and Metabolic Diseases, First Affiliated Hospital, Jinzhou Medical University, Jinzhou, China.

This article presents the case of an 11-year-old girl with a history of Fortan surgery who presented to the authors' department with shortness of breath, orthopnea, and cyanosis. Electrocardiography (ECG) was indicative of mirror-image dextrocardia despite location of the apex impulse on the left. Echocardiography suggested mirror-image dextrocardia accompanied by levoversion, a large atrial septal defect and left ventricular atresia (functional single atrium and single ventricle), and right ventricular hypertrophy. ECG with corrected leads placement showed a sinus rhythm, biatrail enlargement, and right ventricular hypertrophy. Based on echocardiography and medical history, the case was rediagnosed as mirror-image dextrocardia with levoversion.
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http://dx.doi.org/10.1007/s00059-020-04971-7DOI Listing
August 2020

Predictors of Coronavirus Disease 2019 Severity: A Retrospective Study of 64 Cases.

Jpn J Infect Dis 2021 Jan 1;74(1):54-60. Epub 2020 Aug 1.

Treatment and Research Center for Infectious Diseases, Fifth Medical Center of Chinese PLA General Hospital, China.

This study aimed to analyze the clinical characteristics and potential predictors of disease severity in patients with coronavirus disease 2019 (COVID-19). We retrospectively analyzed the clinical data from 64 (37 male and 27 female) patients with COVID-19. Their mean age was 47.8 years; 43 (67.2%) cases were non-severe, 21 (32.8%) were severe, and 2 patients (3.1%) died. Age and serum ferritin levels were significantly associated with COVID-19 severity. There were no significant differences in the duration of severe illness or the number of days on high-level respiratory support between the low-dose and high-dose methylprednisolone groups. The mean number of days in hospital in the high-dose group was higher than that in the low-dose group. Repeated monitoring of ferritin, interleukin-6, C-reactive protein, lactic acid dehydrogenase, and erythrocyte sedimentation rate during COVID-19 treatment may assist in the prediction of disease severity and evaluation of treatment effects.
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http://dx.doi.org/10.7883/yoken.JJID.2020.298DOI Listing
January 2021

Ginsenoside Rb1 Alleviated High-Fat-Diet-Induced Hepatocytic Apoptosis via Peroxisome Proliferator-Activated Receptor .

Biomed Res Int 2020 13;2020:2315230. Epub 2020 Jul 13.

Department of Endocrinology, Affiliated Zhongda Hospital of Southeast University, China.

Objective: High-fat-diet- (HFD-) induced hepatic cell apoptosis is common in mice with nonalcoholic fatty liver disease (NAFLD). We aim to investigate the effect of Ginsenoside Rb1 (GRb1) on hepatocyte apoptosis.

Methods: C57BL/6J mice with HFD were used to induce a liver-injured model with cell apoptosis. In addition, GRb1 was used to treat HFD-induced apoptosis in a liver with or without inhibitor of peroxisome proliferator-activated receptor (PPAR-).

Results: Compared with C57BL/6J mice with common chow, there are downregulated PPAR- but upregulated cell apoptosis in the liver of mice with HFD. Furthermore, GRb1 elevated the hepatic PPAR- level and suppressed hepatocytic apoptosis. However, GW9662 abolished the effects of GRb1 on apoptosis in the liver.

Conclusions: GRb1 alleviated HFD-induced apoptosis of hepatocytes of mice via PPAR-.
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http://dx.doi.org/10.1155/2020/2315230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376403PMC
July 2020

Impact of the COVID-19 Pandemic on Partner Relationships and Sexual and Reproductive Health: Cross-Sectional, Online Survey Study.

J Med Internet Res 2020 08 6;22(8):e20961. Epub 2020 Aug 6.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, National Health Commission Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Hefei, China.

Background: In the past few months, the coronavirus disease (COVID-19) pandemic has caused extensive economic and social damage.

Objective: The purpose of this study was to assess the impact of COVID-19-related measures on partner relationships and sexual and reproductive health in China.

Methods: From May 1 to 5, 2020, 3500 young Chinese individuals were recruited through WeChat or Weibo to participate in a survey to obtain information on sexual and reproductive health (eg, sexual desire, frequency of sexual intercourse, sexual satisfaction, etc). The questionnaire also collected demographic data (eg, age, race, education, current financial status, sexual orientation, relationship status, etc).

Results: In total, 967 participants were included in the sexual health analysis. Due to the COVID-19 pandemic and related containment measures, 22% of participants (n=212) reported a decrease in sexual desire; 41% (n=396) experienced a decrease in the sexual intercourse frequency; 30% (n=291) reported an increase in the frequency of masturbation; 20% (n=192) reported a decrease in alcohol consumption before or during sexual activities, and 31% (n=298) reported a deterioration in partner relationships during the pandemic. The logistic regression analysis indicated that the following influenced partner relationships: accommodations during the pandemic (P=.046; odds ratio [OR] 0.59; 95% CI 0.30-0.86); exclusive relationship status (yes or no) (P<.001; OR 0.44; 95 % CI 0.27-0.73); sexual desire (P=.02; OR 2.01; 95% CI 1.38-2.97); and sexual satisfaction (P<.001; OR 1.92; 95% CI 1.54-2.50). COVID-19 also caused disruptions in reproductive health services such as prenatal and postnatal care, childbirth and abortion services, contraception availability, and the management of sexually transmitted infections.

Conclusions: Our results show that many young people have wide-ranging issues affecting their sexual and reproductive health due to the COVID-19 pandemic and related containment measures. Strategies and guidelines are needed to safeguard the sexual and reproductive health of young people during this pandemic.
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http://dx.doi.org/10.2196/20961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419154PMC
August 2020

Generation of induced pluripotent stem cell GZHMCi001-A and GZHMCi001-B derived from peripheral blood mononuclear cells of epileptic patients with KCNC1 mutation.

Stem Cell Res 2020 Jul 5;47:101897. Epub 2020 Jul 5.

Key Lab for Major Obstetric Diseases of Guangdong Province, Experimental Department of Institute of Gynecology and Obstetrics, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China. Electronic address:

Myoclonus Epilepsy and Ataxia due to Potassium channel mutation (MEAK) is a rare epilepsy caused by changes in the structure and function of potassium channels due to mutations in the potassium voltage-gated channel subfamily C member 1 (KCNC1) gene. MEAK is one of the progressive myoclonus epilepsy (PME), and there are few studies on MEAK pathogenesis and targeted drugs. Here, we used peripheral blood from MEAK patients with KCNC1 (c.959G > A) gene mutation to establish induced pluripotent stem cells (iPSC). The iPSC of KCNC1 mutation established by us is a powerful tool for related research.
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http://dx.doi.org/10.1016/j.scr.2020.101897DOI Listing
July 2020

The safety and effectiveness of genetically corrected iPSCs derived from β-thalassaemia patients in nonmyeloablative β-thalassaemic mice.

Stem Cell Res Ther 2020 07 16;11(1):288. Epub 2020 Jul 16.

Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, Guangdong Province, China.

Background: β-Thalassaemia is a clinically common cause of hereditary haemolytic anaemia stemming from mutations in important functional regions of the β-globin gene. The rapid development of gene editing technology and induced pluripotent stem cell (iPSC)-derived haematopoietic stem cell (HSC) transplantation has provided new methods for curing this disease.

Methods: Genetically corrected β-thalassaemia (homozygous 41/42 deletion) iPSCs that were previously established in our laboratory were induced to differentiate into HSCs, which were transplanted into a mouse model of IVS2-654 β-thalassaemia (B6;129P2-Hbb/J mice) after administration of an appropriate nonmyeloablative conditioning regimen. We also investigated the safety of this method by detecting the incidence of tumour formation in these mice after transplantation.

Results: The combination of 25 mg/kg busulfan and 50 mg/(kg day) cyclophosphamide is an ideal nonmyeloablative protocol before transplantation. Genetically corrected β-thalassaemic HSCs survived and differentiated in nonmyeloablated thalassaemia mice. No tumour formation was observed in the mice for 10 weeks after transplantation.

Conclusion: Our study provides evidence that the transplantation of genetically corrected, patient-specific iPSCs could be used to cure genetic diseases, such as β-thalassaemia major.
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http://dx.doi.org/10.1186/s13287-020-01765-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367314PMC
July 2020

Repeated triplets complicated by monochorionic diamniotic twins following assisted reproduction: a case report and literature review.

BMC Pregnancy Childbirth 2020 Jun 23;20(1):373. Epub 2020 Jun 23.

Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China.

Background: Monochorionic twinning involves numerous maternal and fetal complications, triplets complicated by a monochorionic pair are at further increased risk. Here, we report a case of repeated triplets complicated by monochorionic diamniotic twins with successful pregnancy outcomes in a woman using autologous oocytes.

Case Presentation: A 30-year-old female undergoing embryo transfer with fresh and frozen embryo cycles with autologous oocytes. The two cycles were confirmed by transvaginal ultrasound to result in successful clinical pregnancies of triplets complicated by a monochorionic twinning. The first pregnancy resulted in a singleton delivery after a selective reduction of the monochorionic pair. The subsequent pregnancy resulted in a dichorionic diamniotic twin pregnancy after the heartbeat of one of the monochorionic twin fetuses stopped at 43 days after embryo transfer. Both of the pregnancies ended with successful live births.

Conclusions: Our case report of repeated triplets with monochorionic twins suggests the potential causes and risk factors of monochorionic twinning in assisted reproduction and raises concern regarding the timing of multifetal pregnancy reduction.
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http://dx.doi.org/10.1186/s12884-020-03055-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313207PMC
June 2020