Publications by authors named "Bing Li"

2,754 Publications

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Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework.

Mikrochim Acta 2021 Aug 4;188(8):286. Epub 2021 Aug 4.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.

A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3'-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5'-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface. Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform.
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http://dx.doi.org/10.1007/s00604-021-04912-zDOI Listing
August 2021

Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori.

Environ Pollut 2021 Jul 30;289:117866. Epub 2021 Jul 30.

School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu, 215123, PR China; Sericulture Institute of Soochow University, Suzhou, Jiangsu, 215123, PR China. Electronic address:

The neonicotinoid insecticide acetamiprid is widely applied for pest control in agriculture production, and its exposure often results in adverse effects on a non-target insect, Bombyx mori. However, only few studies have investigated the effects of exposure to sublethal doses of neonicotinoid insecticides on gut microbiota and susceptibility to pathogenic bacteria. In this study, we aimed to explore the possible mechanisms underlying the acetamiprid-induced compositional changes in gut microbiota of silkworm and reduced host resistance against detrimental microbes. This study indicated that sublethal dose of acetamiprid activated the dual oxidase-reactive oxygen species (Duox-ROS) system and induced ROS accumulation, leading to dysregulation of intestinal immune signaling pathways. The evenness and structure of bacterial community were altered. Moreover, after 96 h of exposure to sublethal dose of acetamiprid, several bacteria, such as Pseudomonas sp (Biotype A, DOP-1a, XW34) and Staphylococcus sp (RCB1054, RCB314, X302), invaded the silkworm hemolymph. The survival rate and bodyweight of the acetamiprid treated silkworm larvae inoculated with Enterobacter cloacae (E. cloacae) were significantly lower than the acetamiprid treatment group, suggesting that acetamiprid reduced silkworm resistance against pathogens. These findings indicated that acetamiprid disturbed gut microbial homeostasis of Bombyx mori, resulting in changes in gut microbial community and susceptibility to detrimental microbes.
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http://dx.doi.org/10.1016/j.envpol.2021.117866DOI Listing
July 2021

Prevalence of cleft lip and/or cleft palate in Guangdong province, China, 2015-2018: a spatio-temporal descriptive analysis.

BMJ Open 2021 08 2;11(8):e046430. Epub 2021 Aug 2.

Guangdong Women and Children Hospital, Guangzhou, Guangdong, China

Objectives: This study aimed to investigate the temporal and spatial characteristics of cleft lip and/or palate based on a large-scale birth defect monitoring database.

Methods: Data on perinatal infants and children with cleft lip and/or palate defects from 1 January 2015 to 31 December 2018 in Guangdong province of China were collected. The variables including the demographic data, basic family information (address, education level, etc.), the infant's birth weight, gender and other basic parameters were collected and analysed.

Results: During the study period, the prevalence of cleft lip and/or palate was 7.55 per 10 000 perinatal infants. The prevalence of cleft lip, cleft palate and cleft lip and palate were 2.34/10 000, 2.22/10 000 and 2.98/10 000, respectively. The prevalence of cleft lip and/or palate showed a pronounced downward trend, reducing from 8.47/10 000 in 2015 to 6.51/10 000 in 2018. We observed spatial heterogeneity of prevalence of cleft lip and/or palate across the study period in Guangdong. In the Pearl River Delta region, the overall prevalence of cleft lip and/or palate was 7.31/10 000, while the figure (7.86/10 000) was slightly higher in the non-Pearl River Delta region (p<0.05). Concerning infant gender, the prevalence was in general higher in boys than girls (p<0.05). In addition, the higher prevalence was more common in mothers older than 35 years old. For the birth season, infants born in spring tended to have a higher prevalence than those born in other seasons, regardless of the prevalence of cleft lip and palate calculated separately or jointly (p<0.05). The majority of newborns with cleft lip and palate were accompanied by other birth defects.

Conclusion: This study contributes a better understanding of the characteristics of spatio-temporal trends for birth defects of cleft lip and/or palate in south China.
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http://dx.doi.org/10.1136/bmjopen-2020-046430DOI Listing
August 2021

Cyclic Nucleotide-Gated Ion Channel 6 Mediates Thermotolerance in Seedlings by Regulating Hydrogen Peroxide Production Cytosolic Calcium Ions.

Front Plant Sci 2021 14;12:708672. Epub 2021 Jul 14.

Hebei Key Laboratory of Molecular and Cellular Biology, Key Laboratory of Molecular and Cellular Biology of the Ministry of Education, Hebei Collaboration Innovation Center for Cell Signaling, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

We previously reported the involvement of cyclic nucleotide-gated ion channel 6 (CNGC6) and hydrogen peroxide (HO) in plant responses to heat shock (HS). To demonstrate their relationship with plant thermotolerance, we assessed the effect of HS on several groups of () seedlings: wild-type, mutant, and its complementation line. Under exposure to HS, the level of HO was lower in the mutant seedlings than in the wild-type (WT) seedlings but obviously increased in the complementation line. The treatment of seeds with calcium ions (Ca) increased the HO levels in the seedlings under HS treatment, whereas treatment with a Ca chelator (EGTA) inhibited it, indicating that CNGC6 may stimulate the accumulation of HO in a manner dependent on an increase in cytosolic Ca ([Ca]). This point was verified by phenotypic observations and thermotolerance testing with transgenic plants overexpressing and (two genes involved in HS-responsive HO production), respectively, in a background. Real-time reverse transcription-polymerase chain reactions and Western blotting suggested that CNGC6 enhanced the gene transcription of HS factors (HSFs) and the accumulation of HS proteins (HSPs) HO. These upon results indicate that HO acts downstream of CNGC6 in the HS signaling pathway, increasing our understanding of the initiation of plants responses to high temperatures.
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http://dx.doi.org/10.3389/fpls.2021.708672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317691PMC
July 2021

Non-utilization Is Not the Best Way to Manage Lowland Meadows in Hulun Buir.

Front Plant Sci 2021 16;12:704511. Epub 2021 Jul 16.

Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.

lowland meadow is an important component of natural grasslands in Hulun Buir. However, in Hulun Buir, fewer studies have been conducted on lowland meadows than on other grassland types. To determine the most appropriate utilization mode for lowland meadows, an experiment was conducted in Zhalantun city, Hulun Buir. Unused, moderately grazed, heavily grazed and mowed meadow sites were selected as the research objects. The analysis of experimental data from 4 consecutive years showed that relative to the other utilization modes, mowing and moderate grazing significantly increased biomass. Compared with non-utilization, the other three utilization modes resulted in a higher plant diversity, and the moderately grazed meadow had the highest plant community stability. Moreover, principal component analysis (PCA) showed that among the meadow sites, the mowed meadow had the most stable plant community and soil physicochemical properties. Structural equation modeling (SEM) showed that grazing pressure was less than 0.25 hm/sheep unit and that plant biomass in lowland meadow increases with increasing grazing intensity, temperature and precipitation.
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http://dx.doi.org/10.3389/fpls.2021.704511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322850PMC
July 2021

Decoding Thermal Depolarization Temperature in Bismuth Ferrite-Barium Titanate Relaxor Ferroelectrics with Large Strain Response.

ACS Appl Mater Interfaces 2021 Jul 31. Epub 2021 Jul 31.

Department of Materials Science, Sichuan University, Chengdu 610064, China.

Bismuth ferrite-barium titanate (BF-BT) ferroelectrics attract attention due to their multifunctional properties and potential applications in high-temperature piezodevices. The thermal depolarization temperature () of BF-BT-based relaxor ferroelectrics has a close correlation with electrical properties, which was only recently discovered and is not well recognized. This work is concerned with the thermal depolarization process in (0.67 - )BF-0.33BT-SZ ferroelectrics with large strain response. Macro-to-local property characterization suggests that the largest electrostrain can be achieved in the critical component ( = 0.02) with the most flexible structure features, which is the transition point from the ferroelectric macrodomain to the relaxor nanodomain. The real-space domain image by piezoresponse force microscopy has revealed that an electric field can transform the labyrinth-like nanodomain into oriented large-size domain. Once the heating temperature is above , the poling-induced large-size oriented domain will transform back to its initial state. Most importantly, the thermally induced domain broken is first established with the conduction and phase transition, as disclosed by temperature dependence of DC resistivity and the pyroelectric coefficient. That is, during the thermal depolarization process, the activation energy () changes from 0.65 eV ( < ) to 1.1 eV ( > ), simultaneously accompanied with the structural transition from the poling-induced unstable ferroelectric state (long-range correlated rhombohedral phase) to the initial relaxor state (short-range correlated pseudo-cubic phase), which may be the driving force for the domain decays above . We believe that the understanding of in BF-BT-based relaxor ferroelectrics can provide some clues for further designing high-performance BF-BT ferroelectrics.
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http://dx.doi.org/10.1021/acsami.1c10468DOI Listing
July 2021

Effects of cloransulam-methyl and diclosulam on soil nitrogen and carbon cycle-related microorganisms.

J Hazard Mater 2021 Jun 12;418:126395. Epub 2021 Jun 12.

College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, 61 Daizong Road, Taian 271018, China. Electronic address:

Cloransulam-methyl and diclosulam are applied to soybean fields to control broad-leaved weeds. These herbicides have become a focus of attention because of their low application dose and high-efficiency advantages. However, the effects of these two herbicides on soil microorganisms are unknown. The present study investigated the effects of 0.05, 0.5, and 2.5 mg kg of cloransulam-methyl or diclosulam on soil microbes after 7, 14, 28, 42, and 56 days of exposure. The results showed that the two herbicides increased the abundances of functional bacteria related to pesticide degradation. Based on the genetic expression results, we speculated that 0.05 mg kg of these two herbicides inhibited the nitrification reaction but promoted the denitrification reaction. Diclosulam at a concentration of 0.5 mg kg may enhance the ability of microbes to fix carbon. β-glucosidase activity was activated by the two herbicides at a concentration of 2.5 mg kg. Diclosulam had a positive effect on urease, but cloransulam-methyl activated urease activity only at concentrations of 0.05 and 0.5 mg kg. The results of the integrated biomarker response showed that the toxicity of diclosulam was greater than that of cloransulam-methyl. Our research provides data for evaluating the environmental risks of cloransulam-methyl and diclosulam.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126395DOI Listing
June 2021

Long noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1.

Clin Transl Med 2021 Jul;11(7):e479

National Center for Respiratory Medicine, State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Xi Road, Guangzhou, Guangdong, 510000, China.

Chronic obstructive pulmonary disease is a complex condition with multiple etiologies, including inflammation. We identified a novel long noncoding RNA (lncRNA), interleukin 6 antisense RNA 1 (IL6-AS1), which is upregulated in this disease and is associated with airway inflammation. We found that IL6-AS1 promotes the expression of inflammatory factors, especially interleukin (IL) 6. Mechanistically, cytoplasmic IL6-AS1 acts as an endogenous sponge by competitively binding to the microRNA miR-149-5p to stabilize IL-6 mRNA. Nuclear IL6-AS1 promotes IL-6 transcription by recruiting early B-cell factor 1 to the IL-6 promoter, which increases the methylation of the H3K4 histone and acetylation of the H3K27 histone. We propose a model of lncRNA expression in both the nucleus and cytoplasm that exerts similar effects through differing mechanisms, and IL6-AS1 probably increases inflammation via multiple pathways.
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http://dx.doi.org/10.1002/ctm2.479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288003PMC
July 2021

Interaction-Aware Spatio-Temporal Pyramid Attention Networks for Action Classification.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 27;PP. Epub 2021 Jul 27.

For CNN-based visual action recognition, the accuracy may be increased if local key action regions are focused on. The task of self-attention is to focus on key features and ignore irrelevant information. So, self-attention is useful for action recognition. However, current self-attention methods usually ignore correlations among local feature vectors at spatial positions in CNN feature maps. In this paper, we propose an interaction-aware self-attention model which can extract information about the interactions between feature vectors to learn attention maps. Since different layers in a network capture feature maps at different scales, we introduce a spatial pyramid with the feature maps at different layers for attention modeling. The multi-scale information is utilized to obtain more accurate attention scores. These attention scores are used to weight the local feature vectors of the feature maps and then calculate attentional feature maps. Since the number of feature maps input to the spatial pyramid attention layer is unrestricted, we easily extend this attention layer to a spatio-temporal version. Our model can be embedded in any general CNN to form a video-level attention network for action recognition. Experimental results show that our method achieves state-of-the-art results on the datasets UCF101, HMDB51, Kinetics-400, and untrimmed Charades.
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http://dx.doi.org/10.1109/TPAMI.2021.3100277DOI Listing
July 2021

Oxidative stress and DNA damage induced by trifloxystrobin on earthworms (Eisenia fetida) in two soils.

Sci Total Environ 2021 Jul 17;797:149004. Epub 2021 Jul 17.

College of Resources and Environment, Shandong Agricultural University, Key Laboratory of Agricultural Environment in Universities of Shandong, 61 Daizong Road, Taian 271018, China. Electronic address:

Trifloxystrobin is a new type of fungicide, which is extensively used due to its excellent antifungal activity. In this study, oxidative stress and DNA damage induced by trifloxystrobin exposure was evaluated using Eisenia fetida at subchronic toxicity concentrations in artificial soil and brown soil (0.1-2.5 mg/kg). Throughout the exposure period (days 7, 28 and 56), six biochemical indicators including reactive oxygen species (ROS), antioxidant enzymes (SOD and CAT), glutathione S-transferase (GST), lipid peroxidation and DNA damage (8-hydroxydeoxyguanosine) were measured. In addition, the integrated biomarker response (IBR) index was calculated to make comparison of toxicological response between artificial and brown soils. Results indicated that trifloxystrobin can induce oxidative stress and DNA damage to earthworms with subchronic toxicity greater in brown soil compared to artificial soil as determined through integrated calculations for six biochemical indicators. Trifloxystrobin toxicological experiments in artificial soil may not accurately evaluate its toxicity in natural soil ecosystems, as the toxicity of trifloxystrobin to Eisenia fetida was underestimated.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149004DOI Listing
July 2021

A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys.

J Neurosci Methods 2021 Jul 20;362:109291. Epub 2021 Jul 20.

National Resource Center for Non-Human Primates, Kunming Primate Research Center, and National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China. Electronic address:

Background: Ischemic stroke leads to a long-term disability in humans and no efficient clinical therapy exists to date. The middle cerebral artery occlusion (MCAO) model in non-human primates has shown to be of value for translational stroke research. New method In the current study, a photothrombotic (PT) stroke model was established in rhesus monkeys with either a proximal or distal segment of middle cerebral artery (MCA) thrombosis. This study is the first that compares the two approaches of PT stroke in monkeys using behavioral and physiological measurements and MRI scans.

Results: The experiment found that infarct occurred in the MCA target regions, with all monkeys having impaired behavior reflected by deficits in neurologic function, and motor and cognition in object retrieval detour (ORD) task. The monkeys with distal MCA thrombosis developed with sequential photo-irritations of the Sylvian fissure zone, adjacent central anterior gyrus and central posterior gyrus, had similar impairments with respect to behavior and showed a tendency of a small edema volume with proximal MCA thrombosis at days 4 and 7 post PT stroke.

Comparison With Existing Methods: The distal MCA thrombosis developed with sequential photo-irritations might provide a consistent and well-tolerated focal ischemia in rhesus monkeys, compared with other PT stroke models which usually were singly conducted on the animal's motor cortex and had a temporal effect.

Conclusions: The sequentially photo-irritated PT stroke model is a promising ischemic stroke model in rhesus monkey for studying human stroke pathology and physiology and for new therapies development.
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http://dx.doi.org/10.1016/j.jneumeth.2021.109291DOI Listing
July 2021

Studies on stabilization effect of calcium dihydrogen phosphate (Ca(HPO)) -on Lead(Pb)-contaminated Soil.

Int J Phytoremediation 2021 Jul 22:1-7. Epub 2021 Jul 22.

College of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, China.

This study investigated the effect of Ca(HPO) on pH, leaching toxicity and speciations of soil before and after leaching on it. Different amounts of Ca(HPO) were added to Pb-contaminated soil and stabilized for 30 days. The changes of pH and leaching toxicity of Pb-contaminated soil were tracked during that period. The content of Pb in soil before and after leaching was also determined after 30 days of stabilization. Results showed that the pH of the Pb-contaminated soil didn't change much with the addition of-Ca(HPO). When the amount of Ca(HPO) reached to 3 wt%, the leaching toxicity met the standard limiting level of groundwater class III of China. The change of leaching toxicity was found to be mainly affected by the water-soluble fraction and mild acid-soluble fraction of lead. The speciation experiments revealed that the changes on reducible, oxidizable, and residual fractions are significant, while there are only minor changes on the water-soluble and mild acid-soluble fractions. X-ray diffractometry (XRD) analysis showed that Pb(PO) and PbPO substances were generated in the stabilized soil. The stabilization mechanism of Ca(HPO) was mainly attributed to the formation of insoluble Pb phosphate precipitates through interactions between the heavy metal Pb and the Ca(HPO) In such a way the active species of Pb in the soil can be successfully stabilized. At present, the leaching toxicity is currently used for the evaluation of stabilization effect of heavy metal contaminated soil. The speciation distribution of stabilized contaminated soil before and after leaching has rarely been studied, and the research on stabilizing contaminated soil after leaching is less.Therefore, this paper mainly studies the stabilization effect through the speciation changes of contaminated soil before and after leaching, providing a new idea and method for the evaluation of the stabilization effect of contaminated soil remediation.Ca(H2PO4)2 has no significance in pH of contaminated soil: 5.05<pH<5.5.The content of the water-soluble fraction and the mild acid-soluble fraction of Pb were availably reduced by Ca(H2PO4)2.The content of the water-soluble fraction and the mild acid-soluble fraction of Pb has no marked change before and after leaching.The stabilization mechanism of Ca(H2PO4)2 is through interaction between the Pb in the soil and phosphate to form insoluble substances of lead phosphate.Ca(H2PO4)2 has a good effect on the stabilization of lead-contaminated soil.
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http://dx.doi.org/10.1080/15226514.2021.1952926DOI Listing
July 2021

Validation of the renal risk score for antineutrophil cytoplasmic antibody-associated glomerulonephritis in a Chinese population.

Clin Rheumatol 2021 Jul 19. Epub 2021 Jul 19.

Department of Nephrology, The Second Hospital of Jilin University, Changchun, 130041, China.

Introduction: In 2018, a renal risk score of antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis (AAGN) based on estimated glomerular filtration rate (eGFR), normal glomeruli, and tubular atrophy/interstitial fibrosis (TA/IF) was proposed to predict renal outcomes. We aimed to evaluate this renal risk score in a myeloperoxidase (MPO)-ANCA predominant population in Northeast China.

Methods: We retrospectively analyzed the clinicopathologic data of 65 patients biopsy-proven from a Chinese medical center. Each patient was assessed by eGFR, normal glomeruli, and TA/IF, and the renal outcome was evaluated using the renal risk score.

Results: In our study, 95.4% of patients were ANCA positive (78.5% MPO-ANCA positive and 16.9% proteinase 3-ANCA positive). The average follow-up period was 14.3 months. Thirty-four patients (52.3%) reached end-stage renal disease (ESRD). Based on the renal risk score, 8 (12.3%), 31 (47.7%), and 26 (40%) patients were divided into the low-risk, medium-risk, and high-risk groups, respectively. Kaplan-Meier survival curves revealed the high-risk group had worse renal outcomes than the low-risk group (p<0.01) and the medium-risk group (p<0.01), but the renal outcome did not differ between the low-risk and medium-risk groups (p>0.017). Similar results were obtained by the competitive survival analysis. The AUC for 3-year overall ESRD predictions was 0.845. In the regression analysis, the renal risk score was a favorable predictor for the development of ESRD (HR 3.13, 95%CI 1.58-6.19, p=0.001).

Conclusion: The renal risk score is a preferred index that can predict ESRD in Chinese AAGN patients, especially in the high-risk group with worse renal outcomes. Key Points • The eGFR and percentage of normal glomeruli were valuable predictors of renal outcome, whereas TA/IF was not. • We confirmed the renal risk score is a preferred index that can predict ESRD in Chinese AAGN patients. • Based on the renal risk score, the high-risk group had worse renal outcomes than the low-risk group and the medium-risk group.
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http://dx.doi.org/10.1007/s10067-021-05862-wDOI Listing
July 2021

The Effects of Sevoflurane vs. Propofol for Inflammatory Responses in Patients Undergoing Lung Resection: A Meta-Analysis of Randomized Controlled Trials.

Front Surg 2021 2;8:692734. Epub 2021 Jul 2.

Department of Anesthesiology and Perioperative Medicine, Henan University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China.

Inflammatory cytokines are increased during one-lung ventilation in patients undergoing lung resection, and this increase can be fatal. Propofol and sevoflurane are the main anesthetics used for these patients. Unfortunately, there is no consensus on the best choice of an anesthetic agent concerning an inflammatory response in patients undergoing lung resection. This meta-analysis aimed to compare the effects of propofol and sevoflurane on the inflammatory response in patients undergoing lung resection. We searched electronic databases to identify randomized controlled trials comparing the effects of different anesthetics (sevoflurane vs. propofol) on the inflammatory response. The primary outcome concerned the concentration of systemic inflammatory cytokines. The secondary outcomes concerned the concentrations of inflammatory cytokines in the bronchoalveolar lavage (BAL) fluid from the dependent and independent lung. Random effects analysis of the meta-analyses were performed to synthesize the evidence and to assess the concentrations of inflammatory factors in the sevoflurane and propofol groups. Eight trials involving 488 participants undergoing lung resection with one-lung ventilation were included. There was no significant difference in the concentrations of systemic interleukin (IL)-6, IL-10, or tumor necrosis factor α between the sevoflurane and propofol groups. Compared with the propofol group, BAL levels of IL-6 in the dependent ventilated lung were decreased in the sevoflurane group (three trials, 256 participants; standardized mean difference [SMD], -0.51; 95% confidence interval [CI], -0.90 to -0.11; = 0.01; = 46%). The BAL levels of IL-6 in the independent ventilated lung were also decreased by sevoflurane (four trials, 362 participants; SMD, -0.70; 95% [CI], -0.93 to -0.47; < 0.00001; = 0%). There was no difference in the systemic inflammatory response between the sevoflurane and propofol groups. However, compared with propofol, sevoflurane can reduce the local alveolar inflammatory response. Additional research is necessary to confirm whether the inflammatory response is direct or indirect.
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http://dx.doi.org/10.3389/fsurg.2021.692734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282814PMC
July 2021

Effect of acetic acid on struvite precipitation: An exploration of product purity, morphology and reaction kinetics using central composite design.

Chemosphere 2021 Jul 8;285:131486. Epub 2021 Jul 8.

Department of Chemical & Materials Engineering, University of Auckland, Auckland, 1010, New Zealand.

Phosphorus recovery has attracted increasing interest due to the potential depletion of phosphorus resources. One promising solution is to recover phosphorus via struvite precipitation from wastewater or other waste that is in rich of phosphate. However, product quality control during such process is always challenging due to the variation and complexity of wastewater compositions. For example, subcritical wet oxidation (SCWO) effluent is rich in phosphorus and nitrogen but contains a large amount of acetic acid, while its effect on struvite recovery is hardly known. Therefore, central composite design (CCD), considering pH, acetic acid level, Mg level and Ca level, was used to evaluate the effect of acetic acid on struvite purity, phosphorus removal, morphology and reaction kinetics. The experimental data were statistically analysed by analysis of variance (ANOVA) and principal components analysis (PCA). The results indicate that pH and Mg level have a significant impact on phosphorus removal (pH: p-value < 0.0001, Mg: p-value < 0.0001) and struvite purity (pH: p-value = 0.0410, Mg: p-value < 0.0001), Ca level only affects the struvite purity (p-value = 0.0333). The presence of acetic acid, within the studied range (8.77-34.53 mM), has a negligible effect on struvite morphology, phosphorus removal and reaction kinetics, but a slightly positive effect on struvite purity. Findings of this research would be beneficial to determine the feasibility of acetic acid-rich wastewater as a phosphorus source for struvite recovery.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131486DOI Listing
July 2021

Curcumin functions as an anti-inflammatory and antioxidant agent on arsenic-induced hepatic and kidney injury by inhibiting MAPKs/NF-κB and activating Nrf2 pathways.

Environ Toxicol 2021 Jul 17. Epub 2021 Jul 17.

Environment and Non-Communicable Disease Research Center, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, China.

Chronic arsenic exposure has been associated with various toxic effects, especially to the organs of liver and kidney. As a plant polyphenol, curcumin is the most vital bioactive ingredient of turmeric and has a wide range of pharmacological activities. In the present study, we investigated the potential roles of curcumin against arsenic-induced liver and kidney dysfunctions in mice. Curcumin treatment (200 mg/kg) not only decreased the deposition of arsenic in liver and kidney, but also relieved the hepatic and nephritic biochemical indexes (Glutamic oxaloacetic transaminase [AST], Alanine aminotransferase [ALT], albumin, and creatinine) altered by arsenic at doses of 10 and 25 mg/L via drinking water. What's more, curcumin exerted influences on the activities of myeloperoxidase and on the secretion of inflammatory cytokines in liver and kidney tissues. In addition, the levels of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) phosphorylation were declining while NRF2-signaling targets were increasing in mice liver and kidney by curcumin administration. In conclusion, our results here suggest that curcumin could exert both anti-inflammatory and antioxidant functions on arsenic-induced hepatic and kidney injury by inhibiting MAPKs/NF-κB and activating Nrf2 pathways cooperatively.
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http://dx.doi.org/10.1002/tox.23330DOI Listing
July 2021

Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea.

BMC Microbiol 2021 Jul 16;21(1):214. Epub 2021 Jul 16.

Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of the Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Jiangouyan, Qilihe District, 730050, Lanzhou, China.

Background: The natural hosts of Shigella are typically humans and other primates, but it has been shown that the host range of Shigella has expanded to many animals. Although Shigella is becoming a major threat to animals, there is limited information on the genetic background of local strains. The purpose of this study was to assess the presence of virulence factors and the molecular characteristics of S. flexneri isolated from calves with diarrhea.

Results: Fifty-four S. flexneri isolates from Gansun, Shanxi, Qinghai, Xinjiang and Tibet obtained during 2014 to 2016 possessed four typical biochemical characteristics of Shigella. The prevalences of ipaH, virA, ipaBCD, ial, sen, set1A, set1B and stx were 100 %, 100 %, 77.78 %, 79.63 %, 48.15 %, 48.15 and 0 %, respectively. Multilocus variable number tandem repeat analysis (MLVA) based on 8 variable number of tandem repeat (VNTR) loci discriminated the isolates into 39 different MLVA types (MTs), pulsed field gel electrophoresis (PFGE) based on NotI digestion divided the 54 isolates into 31 PFGE types (PTs), and multilocus sequence typing (MLST) based on 15 housekeeping genes differentiated the isolates into 7 MLST sequence types (STs).

Conclusions: The findings from this study enrich our knowledge of the molecular characteristics of S. flexneri collected from calves with diarrhea, which will be important for addressing clinical and epidemiological issues regarding shigellosis.
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http://dx.doi.org/10.1186/s12866-021-02277-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285881PMC
July 2021

The pharmacodynamic effect of terlipressin versus high-dose octreotide in reducing hepatic venous pressure gradient: a randomized controlled trial.

Ann Transl Med 2021 May;9(9):793

Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Vasoactive drugs can reduce portal venous pressure and control variceal bleeding. However, few studies have explored the hemodynamic effects of terlipressin and high-dose octreotide in such patients. Our purpose was to evaluate the hemodynamic changes and safety of using terlipressin and high-dose octreotide in patients with decompensated liver cirrhosis.

Methods: A multi-center randomized controlled trial was conducted. Cirrhotic patients with a history of variceal bleeding were included. Terlipressin or high-dose octreotide was administered during the procedure of measuring hepatic venous pressure gradient (HVPG). Hemodynamic parameters and symptoms were recorded.

Results: A total of 88 patients were included. HVPG was significantly reduced at 10, 20, and 30 min after drug administration in the terlipressin group (16.3±6.4 14.7±5.9, 14.0±6.1, and 13.8±6.1, respectively, P<0.001) and the high-dose octreotide group (17.4±6.6 15.1±5.8, 15.3±6.2, and 16.1±6.0, respectively P<0.01). Decreased heart rate and increased mean arterial pressure were more often observed in the terlipressin group. The overall response rates were not significantly different between the groups (52.8% 44.8%, P=0.524). The terlipressin group had significantly higher response rates at 30 min compared to the high-dose octreotide group in those with alcoholic liver cirrhosis [6/6 (100%) 0/4 (0%), P=0.005]. The incidence of adverse drug events was rare and similar in the two groups.

Conclusions: Both terlipressin and high-dose octreotide were effective and safe for reducing HVPG. The pharmacodynamic effect of terlipressin persisted longer. The terlipressin group had higher response rates in those with alcoholic cirrhosis (trial number: NCT02119884).
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http://dx.doi.org/10.21037/atm-20-6774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246168PMC
May 2021

Should surgical drainage after lateral transperitoneal laparoscopic adrenalectomy be routine?-A retrospective comparative study.

Gland Surg 2021 Jun;10(6):1910-1919

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Whether to use surgical drains after abdominal surgery or not has received much attention since a hundred years ago. Nowadays, lateral transperitoneal laparoscopic adrenalectomy (LTLA) is a widely used technique to treat adrenal tumors worldwide. However, the placement of drains after LTLA remains controversial.

Methods: Data of 150 patients, who underwent LTLA between October 2014 and September 2020 by the same lead surgeon, were collected, including demographic, pathology, preoperative, operative variables and postoperative complications. The patients were divided into two groups, with and without drainage. The postoperative recovery of the two groups was compared.

Results: Among 150 patients (65 men and 85 women, median age 48 years, median BMI 23.53), 89 patients had no drainage and 61 patients had drainage after surgery. Variables of the two groups were analyzed. Placement of drains correlated with long operative time (P<0.01). Patients with drain had longer hospital stays (P<0.001) and a higher incidence of postoperative complications (P=0.022). Other factors, including tumor size (P=0.61), tumor location (P=0.387), ASA score (P=0.687), pathology (P=0.55), VAS pain score (P=0.41), intraoperative blood loss (P=0.11), were not found to be significantly associated with drain placement. There was no conversion to open surgery in both groups. Moreover, no mortality was observed in either group.

Conclusions: This study revealed that it is feasible and safe not to leave a drain in selective and uncomplicated patients and that surgical drainage should not be routine after LTLA.
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http://dx.doi.org/10.21037/gs-20-829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258894PMC
June 2021

Tumor Microenvironment Modulation Platform Based on Composite Biodegradable Bismuth-Manganese Radiosensitizer for Inhibiting Radioresistant Hypoxic Tumors.

Small 2021 Jul 15:e2101015. Epub 2021 Jul 15.

Institute of Bismuth Science, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Solid tumors possess a unique internal environment with high-level thiols (mainly glutathione), over-expressed H O , and low oxygen partial pressure, which severely restrict the radiotherapy (RT) efficacy. To overcome the imperfections of RT alone, there is vital to design a multifunctional radiosensitizer that simultaneously achieves multimodal therapy and tumor microenvironment (TME) regulation. Bismuth (Bi)-based nanospheres are wrapped in the MnO layer to form core-shell-structured radiosensitizer ([email protected]) that can effectively load docetaxel (DTX). The solubility of [email protected] is further improved via folic acid-modified amphiphilic polyethylene glycol (PFA). [email protected] can specifically respond to the TME to realize multimodal therapy. Primarily, the outer MnO layer responds with H O and glutathione to release oxygen and generate •OH, thereby alleviating hypoxia and achieving chemodynamic therapy (CDT). Afterward, the strong coordination between Bi and deprotonated thiol groups in glutathione allows the mesoporous Bi-containing core bonding with glutathione to form a water-soluble complex. These actions conduce [email protected] degradation, further releasing DTX to implement chemotherapy (CHT). In addition, the degradation in vivo and tumor enrichment of [email protected] are explored via T -weighted magnetic resonance and computed tomography imaging. The biodegradable composite [email protected] can simultaneously modulate the TME and achieve multimodal treatment (RT/CDT/CHT) for hypoxic tumors.
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http://dx.doi.org/10.1002/smll.202101015DOI Listing
July 2021

Antithrombotic Management for Atrial Fibrillation Patients Undergoing Percutaneous Coronary Intervention or With Acute Coronary Syndrome: An Evidence-Based Update.

Front Cardiovasc Med 2021 28;8:660986. Epub 2021 Jun 28.

Department of Pharmacy, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, China.

Combined antithrombotic regimens for atrial fibrillation (AF) patients with coronary artery disease, particularly for those who have acute coronary syndrome (ACS) and/or are undergoing percutaneous coronary intervention (PCI), presents a great challenge in the real-world clinical scenario. Conventionally, a triple antithrombotic therapy (TAT), which consists of combined oral anticoagulant therapy to prevent systemic embolism or stroke along with dual antiplatelet therapy to prevent coronary arterial thrombosis (CAT), is used. However, TAT has been associated with a significantly increased risk of bleeding. With the emergence of non-vitamin K antagonist oral anticoagulants (NOACs), randomized controlled trials have demonstrated a better risk-to-benefit ratio of dual antithrombotic therapy (DAT) in combination of a NOAC and with a P2Y12 inhibitor than vitamin K antagonist-based TAT. The results of these studies have impacted the recommendations of current international guidelines, which favor a DAT with a NOAC and P2Y12 inhibitor (especially clopidogrel) in this clinical setting. Additionally, aspirin can be administered during the periprocedural period, while the treatment duration of TAT should be as short as possible. In this article, we summarize the up-to-date evidence regarding antithrombotic regimens for AF patients with PCI or ACS, with a specific focus on the optimal approach and critical discussions of key scientific data and future developments for antithrombotic management in these patients.
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http://dx.doi.org/10.3389/fcvm.2021.660986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273244PMC
June 2021

Afatinib in the treatment of brain metastases of lung cancer with one rare EGFR mutation: a two-case report.

Anticancer Drugs 2021 Jul 12. Epub 2021 Jul 12.

Department of Intervention Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin, China.

Brain metastasis is one of the common distant metastases of lung cancer. The prognosis of patients with brain metastasis is worse and the survival time is shorter. In this report, we described a rare mutation of EGFR G2607A (rs1050171) in two patients over 50 years of age with brain metastasis of lung cancer. These two patients were both treated with afatinib, followed up for 13 months and 45 months respectively. Both patients showed that the tumor subsided, the curative effect was identified as partial response (PR), no recurrence and progress occurred and still being under follow-up. Our study provides a support that afatinib may be a reasonable therapeutic option for patients with brain metastasis of lung cancer.
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http://dx.doi.org/10.1097/CAD.0000000000001138DOI Listing
July 2021

A new sensor based on thieno[2,3-b]quinoline for the detection of In , Fe and F by different fluorescence behaviors.

Luminescence 2021 Jul 13. Epub 2021 Jul 13.

School of chemistry and chemical engineering, University of Jinan, Jinan, China.

Based on thieno[2,3-b]quinoline-2-carbohydrazide and salicylaldehyde, a novel fluorescent probe (L) was designed and synthesized. L could be used as a multifunctional sensor to sequentially detect In and Fe through fluorescence enhancement and fluorescence quenching in DMF/H O buffer solutions. At the same time, L had good anti-interference ability, which could still detect In and Fe well in the presence of other metal ions. For F , it could be detected by enhancing the fluorescence change caused by the introduction of Al . When other anions were present, the detection of F would not be interfered. The detection limits of In , Fe and F were 1.16×10 M, 2.03×10 M and 7.98×10 M, respectively. The complexation model and sensing mechanism between L and In , Fe and F were confirmed by calculating structural optimization and energy optimization using Gaussian 09 software.
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http://dx.doi.org/10.1002/bio.4119DOI Listing
July 2021

PTEN loss promotes oncogenic function of STMN1 via PI3K/AKT pathway in lung cancer.

Sci Rep 2021 Jul 12;11(1):14318. Epub 2021 Jul 12.

Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1. Eastern Jianshe Road, Zhengzhou, 450052, Henan, China.

Among all cancer types, lung cancer has already become the leading cause of cancer-related death around the world. The molecular mechanism understanding this development is still needed to be improved to treat lung cancer. Stathmin (STMN1) was initially identified as a cytoplasmic protein phosphorylated responding to cell signal and controlled cell physiological processes. The dysregulation of STMN1 is found in various kinds of tumors. However, the molecular mechanism of STMN1 regulating lung cancer is still unclear. Here, we found that STMN1 was overexpressed in lung cancer tissues and associated with worse survival rates of lung cancer patients. Inhibition of STMN1 suppressed lung cancer cell growth, migration and invasion, and promoted drug sensitivity. Moreover, PTEN loss promoted STMN1 expression via PI3K/AKT pathway. PTEN loss ameliorated the inhibition of cell growth, migration and invasion, and drug sensitivity induced by STMN1 knockdown in lung cancer. The high expression of STMN1 was negatively correlated with the low expression of PTEN in lung cancer specimens. Overall, our work demonstrated that PTEN regulated the oncogenic function of STMN1 in lung cancer.
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http://dx.doi.org/10.1038/s41598-021-93815-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275769PMC
July 2021

Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities.

Front Environ Sci Eng 2022 30;16(3):35. Epub 2021 Jun 30.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, 100084 China.

The distribution of antibiotic resistance genes (ARGs) has been intensively studied in large-scale wastewater treatment plants and livestock sources. However, small-scale decentralized sewage treatment facilities must also be explored due to their possible direct exposure to residents. In this study, six wastewater treatment facilities in developed rural areas in eastern China were investigated to understand their risks of spreading ARGs. Using metagenomics and network analysis tools, ARGs and bacterial and viral communities were identified in the influent (INF) and effluent (EFF) samples. The dominant ARGs belonged to the bacitracin class, which are different from most of municipal wastewater treatment plants (WWTPs). The dominant hosts of ARGs are in bacterial communities and in viral communities. Furthermore, a positive relationship was found between ARGs and phages. The ARGs significantly correlated with phages were all hosted by specific genera of bacteria, indicating that phages had contributed to the ARG's proliferation in sewage treatment facilities. Paying significant concern on the possible enhanced risks caused by bacteria, viruses and their related ARGs in decentralized sewage treatment facilities is necessary.

Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s11783-021-1469-4 and is accessible for authorized users.
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http://dx.doi.org/10.1007/s11783-021-1469-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255336PMC
June 2021

Liquid-Liquid Phase-Separated Systems from Reversible Gel-Sol Transition of Protein Microgels.

Adv Mater 2021 Jul 8:e2008670. Epub 2021 Jul 8.

Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, CB2 1EW, UK.

Liquid-liquid phase-separated biomolecular systems are increasingly recognized as key components in the intracellular milieu where they provide spatial organization to the cytoplasm and the nucleoplasm. The widespread use of phase-separated systems by nature has given rise to the inspiration of engineering such functional systems in the laboratory. In particular, reversible gelation of liquid-liquid phase-separated systems could confer functional advantages to the generation of new soft materials. Such gelation processes of biomolecular condensates have been extensively studied due to their links with disease. However, the inverse process, the gel-sol transition, has been less explored. Here, a thermoresponsive gel-sol transition of an extracellular protein in microgel form is explored, resulting in an all-aqueous liquid-liquid phase-separated system with high homogeneity. During this gel-sol transition, elongated gelatin microgels are demonstrated to be converted to a spherical geometry due to interfacial tension becoming the dominant energetic contribution as elasticity diminishes. The phase-separated system is further explored with respect to the diffusion of small particles for drug-release scenarios. Together, this all-aqueous system opens up a route toward size-tunable and monodisperse synthetic biomolecular condensates and controlled liquid-liquid interfaces, offering possibilities for applications in bioengineering and biomedicine.
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http://dx.doi.org/10.1002/adma.202008670DOI Listing
July 2021

Using general computational chemistry strategy to unravel the reactivity of emerging pollutants: An example of sulfonamide chlorination.

Water Res 2021 Jun 29;202:117391. Epub 2021 Jun 29.

Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China. Electronic address:

Increasing number of emerging pollutants in environments requires an effective approach which can facilitate the prediction of reactivity and provide insights into the reaction mechanisms. Computational chemistry is exactly the tool to fulfill this demand with its good performance in theoretical investigation of chemical reactions at molecular level. In this study, chlorination of sulfonamide antibiotics is used as an illustration to present a systematic strategy demonstrating how computational chemistry can be applied to investigate the reaction behavior of emerging pollutants. Sulfonamides is a class of micropollutants that contain the common structure of 4-aminobenzenesulfonmaide while differ in their heterocycles. Based on the calculated conceptual DFT indices, the reactive sites of sulfonamide are successfully predicted, which locate on their common structure of 4-aminobenzenesulfonmaide. Therefore, all sulfonamides follow the similar reaction pathway. Product identification by LTQ-Orbitrap MS further verifies the in silico prediction. Three critical pathways are discovered, i.e., S-N bond cleavage, Cl-substitution onto aniline-N, and the following rearrangement to lose -SO- group, among which Cl-substitution is the key step due to its lowest free energy barrier. Heterocycles impact the reaction rate by affecting the electronic density of aniline group. In general, the more electron-donating the heterocycle is, the more readily sulfonamides to be chlorinated.
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http://dx.doi.org/10.1016/j.watres.2021.117391DOI Listing
June 2021

Keratin 17 in psoriasis: Current understanding and future perspectives.

Semin Cell Dev Biol 2021 Jul 3. Epub 2021 Jul 3.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China. Electronic address:

Keratin 17 (K17) is a multifaceted cytoskeletal protein that is not commonly expressed in the epidermis under normal physiological conditions. However, in psoriasis, K17 is overexpressed in the suprabasal layer of the epidermis and plays an important role in the pathogenesis of the disease. In this review, we have summarized our findings and those reported in other studies concerning the pathogenic functions of K17, as well as the mechanisms underlying the increase in K17 expression in psoriasis. K17 exerts both pro-proliferative and pro-inflammatory effects on keratinocytes. Moreover, K17 peptides trigger autoreactive T cells and promote psoriasis-related cytokine production. In turn, these cytokines modulate the expression, stability, and protein-protein interactions of K17 through transcriptional and translational regulation and post-translational modification of K17 in keratinocytes. Thus, a K17/T-cell/cytokine autoimmune loop is implicated in the pathogenesis of psoriasis, which is supported by the fact that therapies targeting K17 have achieved good outcomes in psoriasis-like mouse models. Future perspectives of K17 in psoriasis have also been discussed to provide potential directions for further studies.
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http://dx.doi.org/10.1016/j.semcdb.2021.06.018DOI Listing
July 2021

Effect of sugammadex on the recovery profiles of cardiac patients undergoing non-cardiac surgery.

Chin Med J (Engl) 2021 Jun 30. Epub 2021 Jun 30.

Department of Anesthesiology, Jiangning Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu 211100, China Department of Anesthesiology and Perioperative Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

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http://dx.doi.org/10.1097/CM9.0000000000001599DOI Listing
June 2021

Integrated Physiological and Transcriptomic Analyses Responses to Altitude Stress in Oat ( L.).

Front Genet 2021 17;12:638683. Epub 2021 Jun 17.

Department of Grassland Science, College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.

Oat is an annual gramineous forage grass with the remarkable ability to survive under various stressful environments. However, understanding the effects of high altitude stresses on oats is poor. Therefore, the physiological and the transcriptomic changes were analyzed at two sites with different altitudes, low (ca. 2,080 m) or high (ca. 2,918 m), respectively. Higher levels of antioxidant enzyme activity, reactive oxygen and major reductions in photosynthesis-related markers were suggested for oats at high altitudes. Furthermore, oat yields were severely suppressed at the high altitude. RNA-seq results showed that 11,639 differentially expressed genes were detected at both the low and the high altitudes in which 5,203 up-regulated and 6,436 down-regulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment tests were conducted and a group of major high altitude-responsive pigment metabolism genes, photosynthesis, hormone signaling, and cutin, suberine and wax biosynthesis were excavated. Using quantitative real-time polymerase chain response, we also confirmed expression levels of 20 DEGs (qRT-PCR). In summary, our study generated genome-wide transcript profile and may be useful for understanding the molecular mechanisms of L. in response to high altitude stress. These new findings contribute to our deeper relevant researches on high altitude stresses and further exploring new candidategenes for adapting plateau environment oat molecular breeding.
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http://dx.doi.org/10.3389/fgene.2021.638683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248544PMC
June 2021
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