Publications by authors named "Bing Chen"

1,289 Publications

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Long non-coding RNA H19 protects against intracerebral hemorrhage injuries via regulating microRNA-106b-5p/acyl-CoA synthetase long chain family member 4 axis.

Bioengineered 2021 Dec;12(1):4004-4015

Department of Neurosurgery, Qingdao Eighth People's Hospital, Qingdao, Shandong, China.

Intracerebral hemorrhage (ICH) is one of the most common refractory diseases. Long non-coding RNAs (lncRNAs) play crucial roles in ICH. This study was designed to investigate the role of lncRNA H19 in ICH and the underlying molecular mechanisms involved. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to determine mRNA expression. Cell viability was analyzed using Cell Counting Kit 8 (CCK8). PI staining Flow cytometry and TdT-mediated biotinylated nick end-labeling (TUNEL) assays were performed to determine ferroptosis in brain microvascular endothelial cells (BMVECs). Targeting relationships were predicted using Starbase and TargetScan and verified by RNA pull-down and luciferase reporter gene assays. Western blotting was performed to assess protein expression. LncRNA H19 is highly expressed in ICH model cells. Over-expression of H19 suppressed cell viability and promoted ferroptosis of BMVECs. miR-106b-5p is predicted to be a target of H19. The expression of miR-106b-5p was lower in oxygen and glucose deprivation hemin-treated (OGD/H-treated) cells. Over-expression of miR-106b-5p reversed the effects of H19 on cell viability and ferroptosis in BMVECs. Furthermore, acyl-CoA synthetase long-chain family member 4 () was verified to be a target gene of miR-106b-5p and was highly expressed in OGD/H-treated cells. Upregulation of ACSL4 inhibited the effects of miR-106b-5p and induced BMVEC dysfunction. In conclusion, lncRNA H19 was overexpressed in ICH. Knockdown of H19 promoted cell proliferation and suppressed BMVECs ferroptosis by regulating the miR-106b-5p/ACSL4 axis. Therefore, H19 knockdown may be a promising therapeutic strategy for ICH.
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http://dx.doi.org/10.1080/21655979.2021.1951070DOI Listing
December 2021

HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein.

Adv Sci (Weinh) 2021 07 1;8(13):2001701. Epub 2021 May 1.

Department of Integrative Oncology Fudan University Shanghai Cancer Center Shanghai Key Laboratory of Medical Epigenetics Institutes of Biomedical Sciences Shanghai Medical College Fudan University Shanghai 200032 China.

Circular RNAs (circRNAs) are an intriguing class of widely prevalent endogenous RNAs, the vast majority of which have not been characterized functionally. Here, we identified a novel oncogenic circRNA originating from the back-splicing of Exon2 and Exon3 of a tumor suppressor gene, ARHGAP35 (also known as P190-A), termed as circARHGAP35. have observe that circARHGAP35 and linear ARHGAP35 have antithetical expression and functions. Interestingly, circARHGAP35 contains a 3867 nt long ORF with an mA-modified start codon and encodes a truncated protein comprising four FF domains and lacking the Rho GAP domain. Mechanistically, circARHGAP35 protein promotes cancer cell progression by interacting with TFII-I protein in the nucleus. The RNA binding protein, HNRNPL, facilitates the formation of circARHGAP35. Clinically, circARHGAP35 is associated with poor survival in cancer patients. Our findings characterize an oncogenic circRNA and demonstrate a novel mechanism of oncogene activation in cancer by circRNA through the production of a truncated protein.
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http://dx.doi.org/10.1002/advs.202001701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261482PMC
July 2021

Identification of Koumine as a Translocator Protein 18 kDa Positive Allosteric Modulator for the Treatment of Inflammatory and Neuropathic Pain.

Front Pharmacol 2021 24;12:692917. Epub 2021 Jun 24.

Department of Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou, China.

Koumine is an alkaloid that displays notable activity against inflammatory and neuropathic pain, but its therapeutic target and molecular mechanism still need further study. Translocator protein 18 kDa (TSPO) is a vital therapeutic target for pain treatment, and recent research implies that there may be allostery in TSPO. Our previous competitive binding assay hint that koumine may function as a TSPO positive allosteric modulator (PAM). Here, for the first time, we report the pharmacological characterization of koumine as a TSPO PAM. The results imply that koumine might be a high-affinity ligand of TSPO and that it likely acts as a PAM since it could delay the dissociation of H-PK11195 from TSPO. Importantly, the allostery was retained , as koumine augmented Ro5-4864-mediated analgesic and anti-inflammatory effects in several acute and chronic inflammatory and neuropathic pain models. Moreover, the positive allosteric modulatory effect of koumine on TSPO was further demonstrated in cell proliferation assays in T98G human glioblastoma cells. In summary, we have identified and characterized koumine as a TSPO PAM for the treatment of inflammatory and neuropathic pain. Our data lay a solid foundation for the use of the clinical candidate koumine to treat inflammatory and neuropathic pain, further demonstrate the allostery in TSPO, and provide the first proof of principle that TSPO PAM may be a novel avenue for the discovery of analgesics.
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http://dx.doi.org/10.3389/fphar.2021.692917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264504PMC
June 2021

Copy number variation in the CES1 gene and the risk of non-alcoholic fatty liver in a Chinese Han population.

Sci Rep 2021 Jul 7;11(1):13984. Epub 2021 Jul 7.

Department of Epidemiology and Health Statistics, Fujian Provincial Key Laboratory of Environment Factors and Cancer, School of Public Health, Fujian Medical University, Fujian, 350122, China.

A recent genome-wide copy number variations (CNVs) scan identified a 16q12.2 deletion that included the carboxylesterase 1 (CES1) gene, which is important in the metabolism of fatty acids and cholesterol. We aimed to investigate whether CES1 CNVs was associated with susceptibility to non-alcoholic fatty liver disease (NAFLD) in a Chinese Han population. A case-control study was conducted among 303 patients diagnosed with NAFLD and 303 age (± 5) and sex-matched controls from the Affiliated Nanping First Hospital of Fujian Medical University in China. The copy numbers of CES1 were measured using TaqMan quantitative real-time polymerase chain reaction (qPCR) and serum CES1 was measured using enzyme-linked immunosorbent assays. The Chi-squared test and a logistic regression model were used to evaluate the association between CES1 CNVs and NAFLD susceptibility. The distribution of CES1 CNVs showed a higher frequency of CNVs loss (< 2) among patients; however, the difference was not significant (P = 0.05). After controlling for other known or suspected risk factors for NAFLD, CES1 CNVs loss was significantly associated with greater risk of NAFLD (adjusted OR = 2.75, 95% CI 1.30-5.85, P = 0.01); while CES1 CNVs gain (> 2) was not. There was a suggestion of an association between increased CES1 serum protein levels and CNVs losses among cases, although this was not statistically significant (P = 0.07). Copy number losses (< 2) of CES1 contribute to susceptibility to NAFLD in the Chinese Han population.
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http://dx.doi.org/10.1038/s41598-021-93549-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263572PMC
July 2021

Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation.

Biochemistry 2021 07 2;60(27):2153-2169. Epub 2021 Jul 2.

School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, U.K.

A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity. The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity among the many different vaccine candidates under investigation. Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against S protein from infectious virus, cultured in Vero cells. We find patterns that are conserved across all samples, and this can be associated with site-specific stalling of glycan maturation that acts as a highly sensitive reporter of protein structure. Molecular dynamics simulations of a fully glycosylated spike support a model of steric restrictions that shape enzymatic processing of the glycans. These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.
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http://dx.doi.org/10.1021/acs.biochem.1c00279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262170PMC
July 2021

A Tri-Satellite Interference Source Localization Method for Eliminating Mirrored Location.

Sensors (Basel) 2021 Jun 30;21(13). Epub 2021 Jun 30.

National Laboratory of Radar Signal Processing, Xidian University, Xi'an 710071, China.

With the increase in satellite communication interference, the tri-satellite time difference of arrival (TDOA) localization technique, which is an effective method to determine the location of the interference using sensors or antennas, has been developed rapidly. The location of the interference source is determined through the intersection of the TDOA lines of position (LOP). However, when the two TDOA LOP have two mirrored intersection points, it is theoretically difficult to determine the real location. Aiming at this problem, a method for eliminating mirrored location based on multiple moment TDOA is proposed in this paper. First, the TDOA results are measured at multiple moments using the cross-ambiguity function (CAF), and the localization equation set is established based on the World Geodetic System (WGS)-84 earth ellipsoid model. Then, the initial location result can be obtained by solving the equation set through the Newton iteration method. Finally, the high-precision location result after eliminating the mirrored location is obtained after the single moment localization based on the initial location. Simulation experiments and real measured data processing results verify the effectiveness of the proposed method. It still has good robustness under the condition of large measurement errors and deviations from the prior initial values.
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http://dx.doi.org/10.3390/s21134483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271526PMC
June 2021

Does Technological Innovation Promote Green Development? A Case Study of the Yangtze River Economic Belt in China.

Int J Environ Res Public Health 2021 06 5;18(11). Epub 2021 Jun 5.

Center for Modern Chinese City Studies, East China Normal University, Shanghai 200062, China.

The role of technological innovation (TI) in green development is controversial. Based on 2003-2017 panel data of 108 cities in the Yangtze River Economic Belt (YREB), this study constructed an index system to evaluate urban green development and analyzed the role of TI on urban green development with the help of a panel econometric model. The results show that: (1) From 2003 to 2017, the levels of TI and green development of cities in the YREB have gradually improved, but the core-periphery structure is obvious, and the levels of TI and green development in the lower reaches are significantly higher than those in the middle and upper reaches. (2) TI has a significant positive role in promoting green development, showing a U-shaped nonlinear relationship, and this relationship varies from region to region. (3) TI has a significant impact on green development with direct and indirect effects. In the economic and social dimensions, TI has a positive impact on green development, while in the ecological dimension, the direct effect and indirect effect have opposite relationships. (4) TI has a significant threshold effect on green development, and there are differences in threshold characteristics between the three dimensions. These findings provide a scientific basis for policymaking about innovation-driven regional green development, and it can enrich the related theories of environmental economic geography.
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http://dx.doi.org/10.3390/ijerph18116111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201148PMC
June 2021

TCF3 is epigenetically silenced by EZH2 and DNMT3B and functions as a tumor suppressor in endometrial cancer.

Cell Death Differ 2021 Jun 26. Epub 2021 Jun 26.

The State Key Laboratory of Pharmaceutical Biotechnology, Department of Hematology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, China-Australia Institute of Translational Medicine, School of Life Sciences, Nanjing University, Nanjing, China.

Endometrial cancer (EC) is the most common gynecological malignancy worldwide. However, the molecular mechanisms underlying EC progression are still largely unknown, and chemotherapeutic options for EC patients are currently very limited. In this study, we found that histone methyltransferase EZH2 and DNA methyltransferase DNMT3B were upregulated in EC samples from patients, and promoted EC cell proliferation as evidenced by assays of cell viability, cell cycle, colony formation. Mechanistically, we found that EZH2 promoted EC cell proliferation by epigenetically repressing TCF3, a direct transcriptional activator of CCKN1A (p21), in vitro and in vivo. In addition, we found that DNMT3B specifically methylated the TCF3 promoter, repressing TCF3 expression and accelerating EC cell proliferation independently of EZH2. Importantly, elevated expression of EZH2 or DNMT3B in EC patients inversely correlated with expression of TCF3 and p21, and was associated with shorter overall survival. We show that combined treatment with GSK126 and 5-Aza-2d treatment wit synergistically inhibited methyltransferase activity of EZH2 and DNMT3B, resulting in a profound block of EC cell proliferation as well as EC tumor progression in cell line-derived xenograft (CDX) and patient-derived xenograft (PDX) mouse models. These findings reveal that TCF3 functions as a tumor suppressor epigenetically silenced by EZH2 and DNMT3B in EC, and support the notion that targeting the EZH2/DNMT3B/TCF3/p21 axis may be a novel and effective therapeutic strategy for treatment of EC.
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http://dx.doi.org/10.1038/s41418-021-00824-wDOI Listing
June 2021

Structural basis for enhanced infectivity and immune evasion of SARS-CoV-2 variants.

Science 2021 Jun 24. Epub 2021 Jun 24.

Division of Molecular Medicine, Boston Children's Hospital, 3 Blackfan Street, Boston, MA 02115, USA.

Several fast-spreading variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have become the dominant circulating strains in the COVID-19 pandemic. We report here cryo-EM structures of the full-length spike (S) trimers of the B.1.1.7 and B.1.351 variants, as well as their biochemical and antigenic properties. Amino acid substitutions in the B.1.1.7 protein increase the accessibility of its receptor binding domain and also the binding affinity for receptor angiotensin-converting enzyme 2 (ACE2). The enhanced receptor engagement may account for the increased transmissibility. The B.1.351 variant has evolved to reshape antigenic surfaces of the major neutralizing sites on the S protein, making it resistant to some potent neutralizing antibodies. These findings provide structural details on how SARS-CoV-2 has evolved to enhance viral fitness and immune evasion.
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http://dx.doi.org/10.1126/science.abi9745DOI Listing
June 2021

Label-free classification of dead and live colonic adenocarcinoma cells based on 2D light scattering and deep learning analysis.

Cytometry A 2021 Jun 19. Epub 2021 Jun 19.

School of Information Engineering, Zhengzhou University, Zhengzhou, China.

The measurement of cell viability plays an essential role in the area of cell biology. At present, the common methods for cell viability assay mainly on the responses of cells to different dyes. However, the additional steps of cell staining will consequently cause time-consuming and laborious efforts. Furthermore, the process of cell staining is invasive and may cause internal structure damage of cells, restricting their reuse in subsequent experiments. In this work, we proposed a label-free method to classify live and dead colonic adenocarcinoma cells by 2D light scattering combined with the deep learning algorithm. The deep convolutional network of YOLO-v3 was used to identify and classify light scattering images of live and dead HT29 cells. This method achieved an excellent sensitivity (93.6%), specificity (94.4%), and accuracy (94%). The results showed that the combination of 2D light scattering images and deep neural network may provide a new label-free method for cellular analysis.
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http://dx.doi.org/10.1002/cyto.a.24475DOI Listing
June 2021

Access-dispersion-recovery strategy for enhanced mitigation of heavy crude oil pollution using magnetic nanoparticles decorated bacteria.

Bioresour Technol 2021 Jun 12;337:125404. Epub 2021 Jun 12.

The Northern Region Persistent Organic Pollution (NRPOP) Control Laboratory, Faculty of Engineering and Applied Science, Memorial University, St. John's, NL A1B 3X5, Canada.

Heavy crude oil (HCO) pollution has gained global attention, but traditional bioremediating practices demonstrate limited effectiveness. This study developed magnetic nanoparticles decorated bacteria (MNPB) using an oil-degrading and biosurfactant-producing Rhodococcus erythropolis species and identified a novel access-dispersion-recovery strategy for enhanced HCO pollution mitigation. The strategy entails (1) magnetic navigation of the MNPB towards HCO layer, (2) enhanced oil dispersion and formation of suspended oil-bacteria aggregates, and (3) magnetic recovery of these aggregates. The UV-spectrophotometer analysis showed that this strategy can enable up to 62% removal of HCO. The GC-MS analysis demonstrated that the MNPB enhanced the degradation of low-molecular-weight aromatics comparing with the pure bacteria, and the recovery process further removed oil-bacteria aggregates and entrained high-molecular-weight aromatics. The feasibility of using MNPB to mitigate HCO pollution could shed light on the emerging bioremediation applications.
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http://dx.doi.org/10.1016/j.biortech.2021.125404DOI Listing
June 2021

Effect of modified bamboo lignin replacing part of C5 petroleum resin on properties of polyurethane/polysiloxane pressure-sensitive adhesive and its application on the wood substrate.

J Colloid Interface Sci 2021 Jun 8;602:394-405. Epub 2021 Jun 8.

Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; CASH GCC (Nanxiong) Research Institute of New Materials Co., Ltd., Shaoguan 512026, PR China.

This paper reports a fresh and robust strategy to develop polyurethane/polysiloxane pressure-sensitive adhesives (PSAs) with excellent properties by replacing part of C5 petroleum resin with modified lignin. A unique aspect of this work is the use of renewable lignin to obtain modified monomers. The phenolic hydroxyl group of lignin is increased by 21.4% after demethylation, which will help to introduce 6-bromo-1-hexene into the lignin structure through Williamson method. The L lignin and C5 petroleum resin are mixed with polyurethane/polysiloxane prepolymer, and furthermore a series of PSAs are obtained under ultraviolet light. It turns out that L lignin can not only replace part of C5 petroleum resin, but also obtain attractive and controllable features. Especially when the mass ratio of C5 petroleum resin to L lignin is 6:4, compared with pure C5 petroleum resin, the 180° peel strength and the shear strength of PU46 are increased by 24.1% and 91.5% respectively. Additionally, the shear strength on the wood substrate is increased by 320.6%. This study provides an effective method for the preparation of high value-added lignin PSA, and expands the application fields of PSA.
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http://dx.doi.org/10.1016/j.jcis.2021.06.033DOI Listing
June 2021

Prescribed Finite-Time Adaptive Neural Tracking Control for Nonlinear State-Constrained Systems: Barrier Function Approach.

IEEE Trans Neural Netw Learn Syst 2021 Jun 14;PP. Epub 2021 Jun 14.

The purpose of this article is to present a novel backstepping-based adaptive neural tracking control design procedure for nonlinear systems with time-varying state constraints. The designed adaptive neural tracking controller is expected to have the following characters: under its action: 1) the designed virtual control signals meet the constraints on the corresponding virtual control states in order to realize the backstepping design ideal and 2) the output tracking error tends to a sufficiently small neighborhood of the origin with the prescribed finite time and accuracy level. By combining the barrier Lyapunov function approach with the adaptive neural backstepping technique, a novel adaptive neural tracking controller is proposed. It is shown that the constructed controller makes sure that the output tracking error converges to a small neighborhood of the origin with the prespecified tracking accuracy and settling time. Finally, the proposed control scheme is further tested by simulation examples.
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http://dx.doi.org/10.1109/TNNLS.2021.3085324DOI Listing
June 2021

Hyperthyroidism Prevalence in China After Universal Salt Iodization.

Front Endocrinol (Lausanne) 2021 28;12:651534. Epub 2021 May 28.

Zhejiang Center for Disease Control and Prevention (Zhejiang CDC), Hangzhou, China.

Background: Universal salt iodization (USI) was implemented in mainland China in 1996. The prevalence of hyperthyroidism and its risk factors now require examination.

Methods: Data were acquired from a nationwide Thyroid, Iodine, and Diabetes Epidemiological survey (TIDE 2015-2017) of 78,470 subjects from 31 provinces. Iodine status, and thyroid hormones and antibodies were measured.

Results: After two decades of USI, the prevalence of overt hyperthyroidism (OH), Graves' disease (GD), severe subclinical hyperthyroidism (severe SCH), and mild subclinical hyperthyroidism (mild SCH) in mainland China was 0.78%, 0.53%, 0.22%, and 0.22%, respectively. OH and GD prevalence were higher in women than in men (OH: 1.16% . 0.64%, P<0.001; GD: 0.65% . 0.37%, P<0.001).Prevalence was significantly decreased after 60 years-of-age compared with 30-39 years-of-age (OH:0.61% . 0.81%, P<0.001; GD: 0.38% . 0.57%, P<0.001).Excessive iodine(EI) and deficient iodine(DI) were both related to increased prevalence of OH (odds ratio [OR] 2.09, 95% confidence interval [CI] 1.68-2.59; OR1.35, 95%CI 1.07-1.72, respectively); however, only deficient iodine was associated with increased prevalence of GD (OR1.67, 95%CI 1.30-2.15). Increased thyroid peroxidase antibody and thyroglobulin antibody levels were significantly associated with prevalence of OH and GD, but not severe SCH and mild SCH. Although hyperthyroidism was more prevalent in women, the association disappeared after adjusting for other factors such as antibody levels.

Conclusion: OH and GD prevalences in mainland China are stable after two decades of USI. Iodine deficiency, elevated thyroid antibody levels, and middle age are the main risk factors for OH and GD. The severe SCH population, rather than the mild SCH population, shows similar characteristics to the OH population.
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http://dx.doi.org/10.3389/fendo.2021.651534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194401PMC
May 2021

A data-driven binary-classification framework for oil fingerprinting analysis.

Environ Res 2021 Jun 8;201:111454. Epub 2021 Jun 8.

Northern Region Persistent Organic Pollutant Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University, St. John's, NL, A1B 3X5, Canada.

A marine oil spill is one of the most challenging environmental issues, resulting in severe long-term impacts on ecosystems and human society. Oil dispersants are widely applied as a treating agent in oil spill response operations. The usage of dispersants significantly changes the behaviors of dispersed oil and consequently challenges the oil fingerprinting analysis. In this study, machine learning was first introduced to analyze oil fingerprinting by developing a data-driven binary classification framework. The modeling integrated dimensionality reduction algorithms (e.g., principal component analysis, PCA) to distinguish. Five groups of biomarkers, including terpanes, steranes, triaromatic steranes (TA-steranes), monoaromatic steranes (MA-steranes), and diamantanes, were selected. Different feature spaces were created from the diagnostic index of biomarkers, and six ML algorithms were applied for comparative analysis and optimizing the modeling process, including k-nearest neighbor (KNN), support vector classifier (SVC), random forest classifier (RFC), decision tree classifier (DTC), logistic regression classifier (LRC), and ensemble vote classifier (EVC). Hyperparameter optimization and cross-validation through GridSearchCV were applied to prevent overfitting and increase the model accuracy. Model performance was evaluated by model score and F-score through confusion matrices. The results indicated that the RFC algorithm from the diamantanes dataset performed the best. It delivered the highest F-score (0.871) versus the lowest F-score (0.792) from the EVC algorithm from the TA-steranes dataset by PCA with a variance of 95%. Therefore, diamantanes were recommended as the most suitable biomarker for distinguishing WCO and CDO to aid oil fingerprinting under the conditions in this study. The results proved the proposed method as a potential analysis tool for oil spill source identification through ML-aided oil fingerprinting. The study also showed the value of ML methods in oil spill response research and practice.
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http://dx.doi.org/10.1016/j.envres.2021.111454DOI Listing
June 2021

3D-QSAR-aided toxicity assessment of synthetic musks and their transformation by-products.

Environ Sci Pollut Res Int 2021 Jun 5. Epub 2021 Jun 5.

Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Civil Engineering, Faculty of Engineering and Applied Science, Memorial University, St. John's, NL, A1B 3X5, Canada.

Synthetic musks (SMs) are fragrance additives widely used in personal care products. SMs and their transformation by-products may reach the environment even after wastewater treatment, resulting in ecological and health concerns. The identification and toxicity assessment of SM by-products generated from different chemical and biological treatment processes have been rarely studied. This study established a 3D-QSAR model based on SMs' molecular structures (independent variable) and their lethal concentration (LC) of mysid (dependent variable). The developed model was further used to predict the LC of SMs transformation by-products. Fifty-eight by-products of six common SMs (i.e., galaxolide (HHCB), tonalide (AHTN), phantolide (PHAN), traseolide (TRASE), celestolide (ADBI), and musk ketone (MK)) generated from biodegradation, photodegradation, advanced oxidation, and chlorination were identified through literature review and lab experiment as the model inputs. Predicted LC results indicated that the toxicity of 40% chlorination by-products is higher than their precursors. Biodegradation is an effective method to treat AHTN. The advanced oxidation may be the best way to treat HHCB. This is the first study on biotoxicity of SM transformation by-products predicted by the 3D-QSAR model. The research outputs helped to provide valuable reference data and guidance to improve management of SMs and other emerging contaminants.
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http://dx.doi.org/10.1007/s11356-021-14672-1DOI Listing
June 2021

Comparison between acupuncture and antidepressant therapy for the treatment of poststroke depression: Systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jun;100(22):e25950

College of Life Sciences, Zhejiang Chinese Medical University, Zhejiang.

Background: In this paper, a systematic review and meta-analysis of published randomized controlled trials (RCTs) was conducted to compare the efficacies of acupuncture and antidepressant therapy for the treatment of poststroke depression (PSD).

Methods: The research team searched RCTs published on PubMed; Medline; Cochrane library; Chinese National Knowledge Infrastructure (CNKI); Wanfang; Embase; Scopus, and Sinomed from their respective establishments to January 2019. We evaluated the Hamilton Depression Rating Scale (HAMD) scores, Treatment Emergent Symptom Scale (TESS) scores, National Institute of Health Stroke Scale (NIHSS) scores, and total clinical efficacy using fixed effects models.

Results: Fourteen RCTs, representing a total of 1124 patients, were studied. Results showed that acupuncture was more effective in improving HAMD scores at 3 weeks after administration (mean difference [MD] = -1.17, 95%CI = -2.18 to -0.16), at 4 weeks (MD = -4.44, 95% CI = -5.64 to -3.23), at 6 weeks (MD = -1.02, 95% CI = -1.68 to -0.36), and at 8 weeks (MD = -4.33, 95% CI = -4.96 to -3.70). Similarly, acupuncture more dramatically decreased NIHSS scores (MD = -2.31, 95% CI = -2.53 to -2.09), and TESS scores (MD = -4.70, 95% CI = -4.93 to -4.48) than conventional Western medicinal therapy. Further, the total clinical efficacy in the acupuncture group was significantly higher than in the antidepressants group (risk ratio [RR] = 1.15, 95% CI = 1.08-1.21).

Conclusions: The results of this study suggest that acupuncture not only can reduce the severity of PSD, but also has significant effects on decreasing the appearance of other adverse events.
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http://dx.doi.org/10.1097/MD.0000000000025950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183854PMC
June 2021

Gata2-L359V impairs primitive and definitive hematopoiesis and blocks cell differentiation in murine chronic myelogenous leukemia model.

Cell Death Dis 2021 Jun 2;12(6):568. Epub 2021 Jun 2.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong University (SJTU) School of Medicine, Shanghai, China.

GATA2, a key transcription factor in hematopoiesis, is frequently mutated in hematopoietic malignancies. How the GATA2 mutants contribute to hematopoiesis and malignant transformation remains largely unexplored. Here, we report that Gata2-L359V mutation impeded hematopoietic differentiation in murine embryonic and adult hematopoiesis and blocked murine chronic myeloid leukemia (CML) cell differentiation. We established a Gata2-L359V knockin mouse model in which the homozygous Gata2-L359V mutation caused major defects in primitive erythropoiesis with an accumulation of erythroid precursors and severe anemia, leading to embryonic lethality around E11.5. During adult life, the Gata2-L359V heterozygous mice exhibited a notable decrease in bone marrow (BM) recovery under stress induction with cytotoxic drug 5-fluorouracil. Using RNA sequencing, it was revealed that homozygous Gata2-L359V suppressed genes related to embryonic hematopoiesis in yolk sac, while heterozygous Gata2-L359V dysregulated genes related to cell cycle and proliferation in BM LinSca1c-kit cells. Furthermore, through chromatin immunoprecipitation sequencing and transactivation experiments, we found that this mutation enhanced the DNA-binding capacity and transcriptional activities of Gata2, which was likely associated with the altered expression of some essential genes during embryonic and adult hematopoiesis. In mice model harboring BCR/ABL, single-cell RNA-sequencing demonstrated that Gata2-L359V induced additional gene expression profile abnormalities and partially affected cell differentiation at the early stage of myelomonocytic lineage, evidenced by the increase of granulocyte-monocyte progenitors and monocytosis. Taken together, our study unveiled that Gata2-L359V mutation induces defective hematopoietic development and blocks the differentiation of CML cells.
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http://dx.doi.org/10.1038/s41419-021-03826-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173010PMC
June 2021

An L314Q mutation in Map3k1 gene results in failure of eyelid fusion in the N-ethyl-N-nitrosourea-induced mutant line.

Exp Anim 2021 Jun 2. Epub 2021 Jun 2.

Institute of Comparative Medicine, Yangzhou University.

In this study, we describe an N-ethyl-N-nitrosourea-induced mouse model with a corneal opacity phenotype that was associated with "eye open at birth" (EOB). Histological and immunohistochemistry staining analysis showed abnormal differentiation of the corneal epithelial cells in the mutant mice. The EOB phenotype was dominantly inherited on a C57BL/6 (B6) background. This allele carries a T941A substitution in exon 4 that leads to an L314Q amino acid change in the open reading frame of MAP3K1 (MEEK1). We named this novel Map3k1 allele Map3k1. Phalloidin staining of F-actin was reduced in the mutant epithelial leading edge cells, which is indicative of abnormality in epithelial cell migration. Interestingly enough, not only p-c-Jun and p-JNK but also c-Jun levels were decreased in the mutant epithelial leading edge cells. This study identifies a novel mouse Map3k1 allele causing EOB phenotype and the EOB phenotype in Map3k1 mousemay be associated with the reduced level of p-JNK and c-Jun.
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http://dx.doi.org/10.1538/expanim.21-0005DOI Listing
June 2021

Development of a Laser Gas Analyzer for Fast CO and HO Flux Measurements Utilizing Derivative Absorption Spectroscopy at a 100 Hz Data Rate.

Sensors (Basel) 2021 May 13;21(10). Epub 2021 May 13.

State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China.

We report the development of a laser gas analyzer that measures gas concentrations at a data rate of 100 Hz. This fast data rate helps eddy covariance calculations for gas fluxes in turbulent high wind speed environments. The laser gas analyzer is based on derivative laser absorption spectroscopy and set for measurements of water vapor (HO, at wavelength ~1392 nm) and carbon dioxide (CO, at ~2004 nm). This instrument, in combination with an ultrasonic anemometer, has been tested experimentally in both marine and terrestrial environments. First, we compared the accuracy of results between the laser gas analyzer and a high-quality commercial instrument with a max data rate of 20 Hz. We then analyzed and compared the correlation of HO flux results at data rates of 100 Hz and 20 Hz in both high and low wind speeds to verify the contribution of high frequency components. The measurement results show that the contribution of 100 Hz data rate to flux calculations is about 11% compared to that measured with 20 Hz data rate, in an environment with wind speed of ~10 m/s. Therefore, it shows that the laser gas analyzer with high detection frequency is more suitable for measurements in high wind speed environments.
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http://dx.doi.org/10.3390/s21103392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152477PMC
May 2021

Single-cell RNA sequencing reveals Nestin active neural stem cells outside the central canal after spinal cord injury.

Sci China Life Sci 2021 May 28. Epub 2021 May 28.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100080, China.

Neural stem cells (NSCs) in the spinal cord hold great potential for repair after spinal cord injury (SCI). The ependyma in the central canal (CC) region has been considered as the NSCs source in the spinal cord. However, the ependyma function as NSCs after SCI is still under debate. We used Nestin as a marker to isolate potential NSCs and their immediate progeny, and characterized the cells before and after SCI by single-cell RNA-sequencing (scRNA-seq). We identified two subgroups of NSCs: the subgroup located within the CC cannot prime to active NSCs after SCI, while the subgroup located outside the CC were activated and exhibited the active NSCs properties after SCI. We demonstrated the comprehensive dynamic transcriptome of NSCs from quiescent to active NSCs after SCI. This study reveals that Nestin cells outside CC were NSCs that activated upon SCI and may thus serve as endogenous NSCs for regenerative treatment of SCI in the future.
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http://dx.doi.org/10.1007/s11427-020-1930-0DOI Listing
May 2021

Xa7, a Small Orphan Gene Harboring Promoter Trap for AvrXa7, Leads to the Durable Resistance to Xanthomonas oryzae Pv. oryzae.

Rice (N Y) 2021 May 30;14(1):48. Epub 2021 May 30.

Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.

Background: The rice (Oryza sativa) gene Xa7 has been hypothesized to be a typical executor resistance gene against Xanthomonas oryzae pv. oryzae (Xoo), and has conferred durable resistance in the field for decades. Its identity and the molecular mechanisms underlying this resistance remain elusive.

Results: Here, we filled in gaps of genome in Xa7 mapping locus via BAC library construction, revealing the presence of a 100-kb non-collinear sequence in the line IRBB7 compared with Nipponbare reference genomes. Complementary transformation with sequentially overlapping subclones of the BACs demonstrated that Xa7 is an orphan gene, encoding a small novel protein distinct from any other resistance proteins reported. A 27-bp effector binding element (EBE) in the Xa7 promoter is essential for AvrXa7-inducing expression model. XA7 is anchored in the endoplasmic reticulum membrane and triggers programmed cell death in rice and tobacco (Nicotiana benthamiana). The Xa7 gene is absent in most cultivars, landraces, and wild rice accessions, but highly homologs of XA7 were identified in Leersia perrieri, the nearest outgroup of the genus Oryza.

Conclusions: Xa7 acts as a trap to perceive AvrXa7 via EBE in its promoter, leading to the initiation of resistant reaction. Since EBE is ubiquitous in promoter of rice susceptible gene SWEET14, the elevated expression of which is conducive to the proliferation of Xoo, that lends a great benefit for the Xoo strains retaining AvrXa7. As a result, varieties harboring Xa7 would show more durable resistance in the field. Xa7 alleles analysis suggests that the discovery of new resistance genes could be extended beyond wild rice, to include wild grasses such as Leersia species.
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http://dx.doi.org/10.1186/s12284-021-00490-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165051PMC
May 2021

Scar tissue removal-activated endogenous neural stem cells aid Taxol-modified collagen scaffolds in repairing chronic long-distance transected spinal cord injury.

Biomater Sci 2021 Jul 27;9(13):4778-4792. Epub 2021 May 27.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Scar tissue removal combined with biomaterial implantation is considered an effective measure to repair chronic transected spinal cord injury (SCI). However, whether more scar tissue removal surgeries could affect the treatment effects of biomaterial implantation still needs to be explored. In this study, we performed the first scar tissue removal surgery in the 3 month and the second in the 6 month after completely removing 1 cm of spinal tissue in canines. We found that Taxol-modified linear ordered collagen scaffold (LOCS + Taxol) implantation could promote axonal regeneration, neurogenesis, and electrophysiological and functional recovery only in canines at the first scar tissue removal surgery, but not in canines at the second scar tissue removal surgery. Interestingly, we found that more endogenous neural stem cells (NSCs) around the injured site could be activated in canines with the first rather than the second scar tissue removal. Furthermore, we demonstrated that Taxol could promote the neuronal differentiation of NSCs in the myelin inhibition microenvironment through the p38 MAPK signaling pathway in vitro. Therefore, we speculated that endogenous NSC activation by the first scar tissue removal surgery and its further differentiation into neurons induced by Taxol may contribute to functional recovery in canines. Together, LOCS + Taxol implantation in combination with the first scar tissue removal provides a promising therapy for chronic long-distance transected SCI repair with the help of scar tissue removal activated endogenous NSCs.
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http://dx.doi.org/10.1039/d1bm00449bDOI Listing
July 2021

M2 macrophage accumulation contributes to pulmonary fibrosis, vascular dilatation, and hypoxemia in rat hepatopulmonary syndrome.

J Cell Physiol 2021 May 27. Epub 2021 May 27.

Department of Anesthesia, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatopulmonary syndrome (HPS) markedly increases the mortality of patients. However, its pathogenesis remains incompletely understood. Rat HPS develops in common bile duct ligation (CBDL)-induced, but not thioacetamide (TAA)-induced cirrhosis. We investigated the mechanisms of HPS by comparing these two models. Pulmonary histology, blood gas exchange, and the related signals regulating macrophage accumulation were assessed in CBDL and TAA rats. Anti-polymorphonuclear leukocyte (antiPMN) and anti-granulocyte-macrophage colony stimulating factor (antiGM-CSF) antibodies, clodronate liposomes (CL), and monocyte chemoattractant protein 1 (MCP1) inhibitor (bindarit) were administrated in CBDL rats, GM-CSF, and MCP1 were administrated in bone marrow-derived macrophages (BMDMs). Pulmonary inflammatory cell recruitment, vascular dilatation, and hypoxemia were progressively developed by 1 week after CBDL, but only occurred at 4 week after TAA. Neutrophils were the primary inflammatory cells within 3 weeks after CBDL and at 4 week after TAA. M2 macrophages were the primary inflammatory cells, meantime, pulmonary fibrosis, GM-CSFR, and CCR2 were specifically increased from 4 week after CBDL. AntiPMN antibody treatment decreased neutrophil and macrophage accumulation, CL or the combination of antiGM-CSF antibody and bindarit treatment decreased macrophage recruitment, resulting in pulmonary fibrosis, vascular dilatation, and hypoxemia in CBDL rats alleviated. The combination treatment of GM-CSF and MCP1 promoted cell migration, M2 macrophage differentiation, and transforming growth factor-β1 (TGF-β1) production in BMDMs. Conclusively, our results highlight neutrophil recruitment mediates pulmonary vascular dilatation and hypoxemia in the early stage of rat HPS. Further, M2 macrophage accumulation induced by GM-CSF/GM-CSFR and MCP1/CCR2 leads to pulmonary fibrosis and promotes vascular dilatation and hypoxemia, as a result, HPS develops.
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http://dx.doi.org/10.1002/jcp.30420DOI Listing
May 2021

Computed tomography image fusion, Coaxial guidewire technique, Fast intraprocedural cortisol testing technique improves success rate and decreases radiation exposure, procedure time, and contrast use for adrenal vein sampling.

J Hypertens 2021 May 25. Epub 2021 May 25.

Department of Vascular Surgery Department of Endocrinology Department of Radiology Department of Laboratory Medicine Department of Clinical Medical Engineering, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China Division of Vascular and Endovascular Surgery, Brigham And Women's Hospital, Boston, Massachusetts, USA.

Background: Adrenal vein sampling (AVS) is recommended for discriminating patients with unilateral primary aldosteronism from bilateral disease. However, it is a technically demanding procedure that is markedly underused. We developed a computed tomography image fusion, coaxial guidewire technique, fast intraprocedural cortisol testing (CCF) technique to improve AVS success rate, which combines CT image fusion, coaxial guidewire technique, and fast intraprocedural cortisol testing.

Objective: To evaluate the effectiveness and safety of the AVS--CCF technique.

Methods: We retrospectively evaluated 105 patients who undervent AVS from June 2016 to October 2020. There were 51 patients in the AVS--CCF group and 54 patients in the AVS group. We compared two groups with technical success rate, procedure time, radiation exposure, volume of contrast medium, and complications (adrenal vein rupture, dissection, infarction, or thrombosis; intraglandular or periadrenal hematoma; and contrast-induced nephropathy).

Results: The technical success rate was higher for AVS--CCF than for AVS without CCF (98 vs. 83.3% for bilateral adrenal veins, P = 0.016). AVS--CCF was associated with a shorter procedure time (63.6 ± 24.6 vs. 94.8 ± 40.8 min, P < 0.001), shorter fluoroscopy time (15.6 ± 12.6 vs. 20.4 ± 15.0 min, P = 0.043), and lower contrast medium volume (25.10 ± 21.82 vs. 44.1 ± 31.0 ml, P < 0.001). There were no significant differences between groups with respect to the time for cannulating the left or right adrenal vein or the peak skin radiation dose. Adrenal vein rupture occurred in 14 patients and intraglandular hematoma in 1 patient.

Conclusion: The CCF technique during AVS not only contributed to improved technical success rates but also associated with decreased procedure time, radiation exposure, and contrast medium volume.
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http://dx.doi.org/10.1097/HJH.0000000000002852DOI Listing
May 2021

Upregulation of Apol8 by Epothilone D facilitates the neuronal relay of transplanted NSCs in spinal cord injury.

Stem Cell Res Ther 2021 05 26;12(1):300. Epub 2021 May 26.

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Microtubule-stabilizing agents have been demonstrated to modulate axonal sprouting during neuronal disease. One such agent, Epothilone D, has been used to treat spinal cord injury (SCI) by promoting axonal sprouting at the lesion site after SCI. However, the role of Epothilone D in the differentiation of neural stem cells (NSCs) in SCI repair is unknown. In the present study, we mainly explored the effects and mechanisms of Epothilone D on the neuronal differentiation of NSCs and revealed a potential new SCI treatment.

Methods: In vitro differentiation assays, western blotting, and quantitative real-time polymerase chain reaction were used to detect the effects of Epothilone D on NSC differentiation. Retrograde tracing using a pseudotyped rabies virus was then used to detect neuronal circuit construction. RNA sequencing (RNA-Seq) was valuable for exploring the target gene involved in the neuronal differentiation stimulated by Epothilone D. In addition, lentivirus-induced overexpression and RNA interference technology were applied to demonstrate the function of the target gene. Last, an Apol8-NSC-linear ordered collagen scaffold (LOCS) graft was prepared to treat a mouse model of SCI, and functional and electrophysiological evaluations were performed.

Results: We first revealed that Epothilone D promoted the neuronal differentiation of cultured NSCs and facilitated neuronal relay formation in the injured site after SCI. Furthermore, the RNA-Seq results demonstrated that Apol8 was upregulated during Epothilone D-induced neuronal relay formation. Lentivirus-mediated Apol8 overexpression in NSCs (Apol8-NSCs) promoted NSC differentiation toward neurons, and an Apol8 interference assay showed that Apol8 had a role in promoting neuronal differentiation under the induction of Epothilone D. Last, Apol8-NSC transplantation with LOCS promoted the neuronal differentiation of transplanted NSCs in the lesion site as well as synapse formation, thus improving the motor function of mice with complete spinal cord transection.

Conclusions: Epothilone D can promote the neuronal differentiation of NSCs by upregulating Apol8, which may provide a promising therapeutic target for SCI repair.
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http://dx.doi.org/10.1186/s13287-021-02375-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157417PMC
May 2021

Relationship between short-term and mid-term hearing outcomes after stapedotomy in patients with otosclerosis: an investigation.

Acta Otolaryngol 2021 Jun 24;141(6):603-607. Epub 2021 May 24.

ENT Institute and Otorhinolaryngology Department, Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.

Background: Although stapedotomy is effective for patients with clinical otosclerosis, the time of hearing stabilization has not yet been consistent.

Objective: To investigate the relationships between post-operative follow-up times, hearing outcomes, and threshold shift after stapedotomy.

Materials And Methods: Fifty-five patients with clinical otosclerosis that underwent stapedotomy were retrospectively studied. Pure tone audiometry tests were conducted within the first month (short-term) and within 1 year (mid-term) postoperatively. Data were analyzed for two rounds of audiometry tests at different postoperative follow-up times.

Results: Air conduction (AC) and bone conduction (BC) were significantly correlated with preoperative hearing levels (<.01). AC, BC, and air bone gap (ABG) significantly improved at the short-term (<.001) and continued to improve at the mid-term (<.01). The success rate of surgery increased from 87% at short-term to 98% at mid-term. Less than 1/3 of cases encountered BC deterioration at short-term, whereas most improved at mid-term.

Conclusions: Hearing results showed a trend of improvement between short-term and mid-term follow-ups after stapedotomy. AC, ABG, and success rate displayed significant improvement several months postoperatively. BC deterioration occurred in less than 30% of patients at short-term. The recovery of BC at 4 kHz was later than that of low frequencies.
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http://dx.doi.org/10.1080/00016489.2021.1909748DOI Listing
June 2021

The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen sponge.

Stem Cell Res Ther 2021 05 21;12(1):298. Epub 2021 May 21.

Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing, 100190, China.

Background: Recently, neural stem cell (NSC) therapy has shown promise for the treatment of many neurological diseases. Enhancing the quality of implanted cells and improving therapeutic efficacy are currently research hotspots. It has been reported that collagen sponge material provided sufficient room for cell growth in all directions and promoted the absorption of nutrients and removal of wastes. And also, the Rotary Cell Culture System (RCCS), which mimics the microgravity environment, can be used to culture cells for tissue engineering.

Materials And Methods: We performed the mRNA and miRNA sequencing to elucidate the regulatory mechanism of NSCs cultured on the collagen sponge in the RCCS system. The luciferase assay and Western blot revealed a direct regulatory role between let-7i-5p and neurotrophic receptor tyrosine kinase 3 (NTRK3; also called TrkC). And then, the neural differentiation markers Tuj1 and Map2 were detected by immunofluorescence staining. In the meantime, the migratory ability of NSCs was detected both in vitro and in spinal cord injury animals.

Results: In this study, we demonstrated that the expression of NTRK3 was elevated in NSCs cultured on collagen sponge in the RCCS system. Furthermore, increased NTRK3 expression was regulated by the downregulation of let-7i-5p. Compared to traditionally cultured NSCs, the NSCs cultured on collagen sponge in the RCCS system exhibited better neuronal differentiation and migratory ability, especially in the presence of NT-3.

Conclusions: As the biological properties and quality of transplanted cells are critical for therapeutic success, the RCCS system combined with the collagen sponge culture system shows promise for applications in clinical practice in the future.
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http://dx.doi.org/10.1186/s13287-021-02381-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139048PMC
May 2021

Laparoscopic side-to-side duodenoduodenostomy versus diamond-shaped anastomosis for annular pancreas in the neonate.

ANZ J Surg 2021 May 20. Epub 2021 May 20.

Department of Pediatric Surgery, Huai'an Women and Children's Hospital, Jiangsu, China.

Background: The aim of this report is to present our experience of the last 5 years with repairs of congenital annular pancreas in neonates to determine the efficacy and outcomes of laparoscopic side-to-side duodenoduodenostomy compared to laparoscopic diamond-shaped anastomosis.

Methods: A total of 35 patients with congenital annular pancreas in neonates were operated by laparoscopic procedure at our hospital during January 2015 and September 2020. Twenty patients underwent laparoscopic side-to-side duodenoduodenostomy (group A), and 15 patients of the control group underwent laparoscopic diamond-shaped anastomosis (group B). The clinical data between the two groups were compared.

Results: The operative time is 77.5 ± 18.7 min in group A (range 50 ~ 125 min), and 92.5 ± 20.2 min in group B (range 75-155 min) (P = 0.029). Feedings started on postoperative day 4.5 ± 0.9 (range 3-6 days) in group A, whereas 6.5 ± 0.8 (range 4-7 days) in group B (P = 0.013). The cases in group A were discharged uneventfully in a mean of 9.2 ± 2.3 (range 7-15 days) postoperative day, and 11.4 ± 3.7 days (range 8-20 days) in group B (P = 0.041). The cases of group A have been followed up for 33.1 ± 15.4 months (range 3-60 months), group B had been followed up for 32.0 ± 14.0 months (range 6-55 months) (P > 0.05), and all the cases were doing well at the last follow-up examination.

Conclusion: Laparoscopic side-to-side duodenoduodenostomy is beneficial to the recovery of intestinal function postoperatively in the neonate with annular pancreas.
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http://dx.doi.org/10.1111/ans.16959DOI Listing
May 2021