Publications by authors named "Bin Zeng"

199 Publications

[An improved method for isolation and culture of primary cardiomyocytes from SD neonatal rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2021 Nov;37(6):699-704

Department of Cardiovascular Disease, Ningbo Medical Treatment Centre Li Huili Hospital, Ningbo 315040, China.

To establish a stable, rapid and improved method for isolation and culture of primary cardiomyocytes from neonatal rats. Ventricular tissues from neonatal SD rats were digested with 0.12% collagenase Ⅱ, and then subjected to Percoll density gradient centrifugation. The original cardiomyocytes were cultured in modified DMEM/F12 containing 5% horse serum and 5-bromodeoxyuracil(5-BrdU) for further purification, and medium was changed to normal high glucose DMEM with 10% FBS the next day. The difference between the improved method and traditional differential attachment one used for isolation and culture of primary cardiomyocytes was compared. Cardiacmyocytes obtained through the improved method grew well. 24 hours after plating, most cells adhered to the dishes, with shapes looked triangular, fusiform or irregular, and some of them showed spontaneously contract at a frequency varying from 10~30 times/min. After 48 h culture, the cardiomyocytes became longer and stretched out pseudopodia. Some cells showed synchronous beats with the frequency close to 50~80 times/min. 72 hours later, cardiomyocytes were interwoven into a network in chrysanthemum patterns, and spontaneous beats tended to be more synchronous, with a frequency of 80-100 times/min. After 96 h, cells gathered into clusters as islands, with synchronous beat at a frequency of around 100~120 times/min. All cardiomyocytes were in good condition within one week. Yields((1.17±0.15)×10 (1.21±0.22)×10,>0.05)and survival rate of primary cardiomyocytes obtained by the improved method was comparable to that gained using traditional differential attachment way (93.3%±1.4% 92.2%±0.7%, >0.05), but the purity of primary cardiomyocytes obtained through the improved method was much higher (94.7%±2.1% 89.5%±1.3%, <0.05), while with less time consuming ((3.1±0.4)h (4.3±0.3)h, <0.01). This improved method is an ideal and simple method for the isolation and culture of primary cardiomyocytes with shorter time-consuming, high purity, intact structure and function, and with great repeatability and stability.
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http://dx.doi.org/10.12047/j.cjap.6063.2021.085DOI Listing
November 2021

Andrographolide: A review of its pharmacology, pharmacokinetics, toxicity and clinical trials and pharmaceutical researches.

Phytother Res 2021 Nov 24. Epub 2021 Nov 24.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacology, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Andrographis paniculata (Burm. f.) Wall. ex Nees, a renowned herb medicine in China, is broadly utilized in traditional Chinese medicine (TCM) for the treatment of cold and fever, sore throat, sore tongue, snake bite with its excellent functions of clearing heat and toxin, cooling blood and detumescence from times immemorial. Modern pharmacological research corroborates that andrographolide, the major ingredient in this traditional herb, is the fundamental material basis for its efficacy. As the main component of Andrographis paniculata (Burm. f.) Wall. ex Nees, andrographolide reveals numerous therapeutic actions, such as antiinflammatory, antioxidant, anticancer, antimicrobial, antihyperglycemic and so on. However, there are scarcely systematic summaries on the specific mechanism of disease treatment and pharmacokinetics. Moreover, it is also found that it possesses easily ignored security issues in clinical application, such as nephrotoxicity and reproductive toxicity. Thereby it should be kept a lookout over in clinical. Besides, the relationship between the efficacy and security issues of andrographolide should be investigated and evaluated scientifically. In this review, special emphasis is given to andrographolide, a multifunctional natural terpenoids, including its pharmacology, pharmacokinetics, toxicity and pharmaceutical researches. A brief overview of its clinical trials is also presented. This review intends to systematically and comprehensively summarize the current researches of andrographolide, which is of great significance for the development of andrographolide clinical products. Noteworthy, those un-cracked issues such as specific pharmacological mechanisms, security issues, as well as the bottleneck in clinical transformation, which detailed exploration and excavation are still not to be ignored before achieving integration into clinical practice. In addition, given that current extensive clinical data do not have sufficient rigor and documented details, more high-quality investigations in this field are needed to validate the efficacy and/or safety of many herbal products.
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http://dx.doi.org/10.1002/ptr.7324DOI Listing
November 2021

Effects of Nitrogen and Phosphorus Limitation on Fatty Acid Contents in .

Front Microbiol 2021 21;12:739569. Epub 2021 Oct 21.

Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, China.

, commonly known as koji mold, has been widely used for the large-scale production of food products (sake, makgeolli, and soy sauce) and can accumulate a high level of lipids. In the present study, we showed the dynamic changes in mycelium growth and conidia formation under nitrogen and phosphorus nutrient stress. The fatty acid profile of was determined and the content of unsaturated fatty acid was found increased under nitrogen and phosphorus limitation. Oleic acid (C), linoleic acid (C), and γ-linolenic acid (C) production were increased on five nitrogen and phosphorus limitation media, especially on nitrogen deep limitation and phosphorus limitation group, showing a 1. 2-, 1. 6-, and 2.4-fold increment, respectively, compared with the control. Transcriptomic analysis showed the expression profile of genes related to nitrogen metabolism, citrate cycle, and linoleic acid synthesis, resulting in the accumulation of unsaturated fatty acid. qRT-PCR results further confirmed the reliability and availability of the differentially expressed genes obtained from the transcriptome analysis. Our study provides a global transcriptome characterization of the nitrogen and phosphorus nutrient stress adaptation process in . It also revealed that the molecular mechanisms of respond to nitrogen and phosphorus stress. Our finding facilitates the construction of industrial strains with a nutrient-limited tolerance.
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http://dx.doi.org/10.3389/fmicb.2021.739569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566876PMC
October 2021

Crystal facet modulation of BiWO microplates for spatial charge separation and inhibiting reverse reaction.

Chem Commun (Camb) 2021 Nov 4;57(88):11637-11640. Epub 2021 Nov 4.

State Key Laboratory of Catalysis, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian, 116023, China.

We experimentally demonstrated that spatial charge separation can take place between the {010} and {001} facets of BiWO microplates. Further assembly of the reduction and oxidation cocatalysts leads to a remarkable enhancement of photocatalytic water oxidation activity in the presence of Fe ions while the reverse oxidation of Fe to Fe ions is totally inhibited. The origin of the driving force is theoretically proven to be the difference in surface work function between the co-exposed facets, which shows a feasible strategy for developing efficient photocatalysts for solar energy conversion.
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http://dx.doi.org/10.1039/d1cc04942aDOI Listing
November 2021

Identification and characterization of Nramp transporter AoNramp1 in .

3 Biotech 2021 Oct 30;11(10):452. Epub 2021 Sep 30.

Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013 China.

The Nramp (natural resistance-associated macrophage protein) family of genes has been identified and characterized widely in many species. However, the genes and their characterizations have not been reported for . Here, only one gene in genome was identified. Phylogenetic analysis revealed that AoNramp1 is not clustered with Nramps from yeast genus. Expression analysis showed that the transcript level of was strongly induced under both Zn/Mn-replete and -deplete conditions. The GUS-staining assay indicated that the expression of was strongly induced by Zn/Mn. Moreover, the deletion and overexpression strains were constructed by the CRISPR/Cas9 system and promoter, respectively. Phenotypic analysis showed that overexpression and deletion of caused growth defects under Zn/Mn-deplete and -replete conditions, including mycelium growth and conidia formation. Together, these findings provide valuable information for further study on the biological roles of in .

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-02998-z.
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http://dx.doi.org/10.1007/s13205-021-02998-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484427PMC
October 2021

Atypical TNF-TNFR superfamily binding interface in the GITR-GITRL complex for T cell activation.

Cell Rep 2021 Sep;36(12):109734

Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a critical regulatory molecule in modulation of T cell immune responses. Here we report the mouse GITR (mGITR) and mGITR ligand (mGITRL) complex structure and find that the binding interface of mGITR and mGITRL is distinct from the typical tumor necrosis factor superfamily (TNFSF)/TNF receptor superfamily (TNFRSF) members. mGITR binds to its ligand with a single domain, whereas the binding interface on mGITRL is located on the side, which is distal from conserved binding sites of TNFSF molecules. Mutational analysis reveals that the binding interface of GITR/GITRL in humans is conserved with that in the mouse. Substitution of key interacting D93-I94-V95 (DIV) in mGITR with the corresponding K93-F94-S95 (KFS) in human GITR enables cross-recognition with human GITRL and cross-activation of receptor signaling. The findings of this study substantially expand our understanding of the interaction of TNFSF/TNFRSF superfamily molecules and can benefit the future design of biologics by targeting GITR/GITRL.
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http://dx.doi.org/10.1016/j.celrep.2021.109734DOI Listing
September 2021

SEC23A Is an Independent Prognostic Biomarker in Bladder Cancer Correlated With MAPK Signaling.

Front Genet 2021 11;12:672832. Epub 2021 Aug 11.

State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded By Chongqing and the Ministry of Science and Technology, School of Biomedical Engineering, Chongqing Medical University, Chongqing, China.

Clinical data mining and bioinformatics analysis can be employed effectively to elucidate the function and underlying mechanisms of the gene of interest. Here, we have proposed a framework for the identification and validation of independent biomarkers in human cancer and for mechanistic profiling using gene sets enrichment analysis and pathway analysis. This is followed by validation with experiments. Using this framework to analyze the clinical relevance of SEC23A, we have discovered the prognostic potential of SEC23A in different cancers and identified SEC23A as an independent prognostic factor for poor prognosis in bladder cancer, which implicates SEC23A, for the first time, as an oncogene. Bioinformatic analyses have elucidated an association between SEC23A expression and the upregulation of the MAPK signaling pathway. Using the T24 human bladder cell line, we confirmed that knockdown of SEC23A expression could effectively impact the MAPK signaling pathway. Further, through PCR verification, we showed that MEF2A, one of the key genes of the MAPK signaling pathway, might be a downstream factor of the SEC23A gene.
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http://dx.doi.org/10.3389/fgene.2021.672832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385657PMC
August 2021

SEC23A Inhibit Melanoma Metastatic through Secretory PF4 Cooperation with SPARC to Inhibit MAPK Signaling Pathway.

Int J Biol Sci 2021 13;17(12):3000-3012. Epub 2021 Jul 13.

Institute of Life Sciences, Chongqing Medical University, Chongqing, China.

Metastasis of melanoma to the distant organs is a multistep process in which the tumor microenvironment (TME) may play an important role. However, the relationship between metastatic progression and TME is intricate. In the present study, using melanoma derivative cell lines OL (oligometastatic) and POL (polymetastatic) that differ in their metastatic colonization capability, we have elucidated a new mechanism involving "SEC23A-PF4-MAPK/ERK axis" in which PF4 transported by COPII hinders metastasis through inhibition of MAPK/ERK signaling pathway. Furthermore, SPARC can act cooperatively to enhance the inhibition of Pf4 on ERK phosphorylation and melanoma cell metastasis. Our findings show the possibility of targeting cancer cell secretome for therapeutic development.
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http://dx.doi.org/10.7150/ijbs.60866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375231PMC
July 2021

Modulating immune microenvironment during bone repair using biomaterials: Focusing on the role of macrophages.

Mol Immunol 2021 10 12;138:110-120. Epub 2021 Aug 12.

Department of Orthopedics, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Zhoushan 316000, Zhejiang Province, PR China. Electronic address:

Bone is a self-regenerative tissue that can repair small defects and fractures. In large defects, bone tissue is unable to provide nutrients and oxygen for repair, and autologous grafting is used as the gold standard. As an alternative method, the bone tissue regeneration approach uses osteoconductive biomaterials to overcome bone graft disadvantages. However, biomaterials are considered as foreign components that can stimulate host immune responses. Although traditional principles have been aimed to minimize immune reactions, the design of biomaterials has steadily shifted toward creating an immunomodulatory microenvironment to harness immune cells and responses to repair damaged tissue. Among immune cells, macrophages secrete various immunomodulatory mediators and crosstalk with bone-forming cells and play key roles in bone tissue engineering. Macrophage polarization toward M1 and M2 subtypes mediate pro-inflammatory and anti-inflammatory responses, respectively, which are crucial for bone repairing at different stages. This review provides an overview of the crosstalk between various immune cells and biomaterials, macrophage polarization, and the effect of physicochemical properties of biomaterials on the immune responses, especially macrophages, in bone tissue engineering.
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http://dx.doi.org/10.1016/j.molimm.2021.08.003DOI Listing
October 2021

Propofol induces hepatocellular carcinoma cell apoptosis via regulating miR-105/JAK2/STAT3 axis.

Cytokine 2021 12 10;148:155649. Epub 2021 Aug 10.

Department of Pediatric General Surgery, The First People's Hospital of Chenzhou, Chenzhou 423000, Hunan Province, PR China. Electronic address:

Background: Hepatocellular carcinoma (HCC) is a type of malignancy with high mortality. It has been reported Propofol could modulate the tumorigenesis of liver cancer; however, the mechanism by which Propofol regulates the development of HCC is still not clear.

Methods: CCK8 assay was applied to test the cell viability. Flow cytometry and TUNEL staining were applied to detect the cell apoptosis. Meanwhile, dual luciferase reporter assay was performed to investigate the association between miR-105 and JAK2. In addition, RNA and protein levels were investigated by qRT-PCR and western blot, respectively.

Results: Propofol significantly suppressed the proliferation of HCC cells via inducing the apoptosis. Consistently, miR-105 upregulation inhibited the proliferation of HCC cells, while downregulation of miR-105 reversed Propofol-induced HCC cell apoptosis. Meanwhile, JAK2 was found to be the direct target of miR-105. Furthermore, Propofol could inactivate JAK2/STAT3 signaling via upregulation of miR-105.

Conclusion: Propofol significantly attenuated HCC tumorigenesis via mediation of miR-105/JAK2/STAT3 axis. Thereby, Propofol might act as a new agent for the treatment of HCC.
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http://dx.doi.org/10.1016/j.cyto.2021.155649DOI Listing
December 2021

Glycosphingolipids in Filamentous Fungi: Biological Roles and Potential Applications in Cosmetics and Health Foods.

Front Microbiol 2021 22;12:690211. Epub 2021 Jul 22.

Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, China.

Filamentous fungi are a group of economically important fungi used in the production of fermented foods, industrial enzymes, and secondary metabolites. Glycosphingolipids (GSLs) as constituents of lipid rafts are involved in growth, differentiation, and response to environment stress in filamentous fungi. In addition to these key roles, GSLs are also important in the barrier function of skin to retain moisture as a moisturizing ingredient in cosmetics or health products for their strong biological activity as a functional component. GSLs found in filamentous fungi are divided in two major classes: neutral GSLs (glycosylceramides), glucosylceramides (GlcCers), and/or galactosylceramides (GalCers) and acidic GSLs, mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP)C]. Glycosylceramides are one of the abundant GSLs in and known to improve skin-barrier function and prevent intestinal impairment as a prebiotic. Some filamentous fungi of spp., synthesizing both GlcCer and GalCer, would be an amenable source to exploit glycosylceramides that wildly adding in cosmetics as moisturizing ingredients or health food as dietary supplements. In this minireview, the types, structures, and biosynthetic pathways of GSLs in filamentous fungi, and the relevance of GSLs in fungal growth, spore formation, and environmental stress response are explained. Furthermore, the advantage, potential development, and application of GlcCer and GalCer from filamentous fungi spp. are also investigate based on the use of plant GlcCer in health foods and cosmetics.
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http://dx.doi.org/10.3389/fmicb.2021.690211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341767PMC
July 2021

Hydrogen-Assisted Brittle Fracture Behavior of Low Alloy 30CrMo Steel Based on the Combination of Experimental and Numerical Analyses.

Materials (Basel) 2021 Jul 2;14(13). Epub 2021 Jul 2.

Key Laboratory of Disaster Prevent and Structural Safety, Guangxi Key Laboratory Disaster Prevent and Engineering Safety, College of Civil Engineering and Architecture, Guangxi University, Nanning 530004, China.

Compact-tension (CT) specimens made of low alloy 30CrMo steels were hydrogen-charged, and then subjected to the fracture toughness test. The experimental results revealed that the higher crack propagation and the lower crack growth resistance (CTOD-R curve) are significantly noticeable with increasing hydrogen embrittlement (HE) indexes. Moreover, the transition in the microstructural fracture mechanism from ductile (microvoid coalescence (MVC)) without hydrogen to a mixed quasi-cleavage (QC) fracture and QC + intergranular (IG) fracture with hydrogen was observed. The hydrogen-enhanced decohesion (HEDE) mechanism was characterized as the dominant HE mechanism. According to the experimental testing, the coupled problem of stress field and hydrogen diffusion field with cohesive zone stress analysis was employed to simulate hydrogen-assisted brittle fracture behavior by using ABAQUS software. The trapezoidal traction-separation law (TSL) was adopted, and the initial TSL parameters from the best fit to the load-displacement and J-integral experimental curves without hydrogen were calibrated for the critical separation of 0.0393 mm and the cohesive strength of 2100 MPa. The HEDE was implemented through hydrogen influence in the TSL, and to estimate the initial hydrogen concentration based on matching numerical and experimental load-line displacement curves with hydrogen. The simulation results show that the general trend of the computational CTOD-R curves corresponding to initial hydrogen concentration is almost the same as that obtained from the experimental data but in full agreement, the computational CTOD values being slightly higher. Comparative analysis of numerical and experimental results shows that the coupled model can provide design and prediction to calculate hydrogen-assisted fracture behavior prior to extensive laboratory testing, provided that the material properties and properly calibrated TSL parameters are known.
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http://dx.doi.org/10.3390/ma14133711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269893PMC
July 2021

A highly efficient identification of mutants generated by CRISPR/Cas9 using the non‑functional DsRed assisted selection in Aspergillus oryzae.

World J Microbiol Biotechnol 2021 Jul 9;37(8):132. Epub 2021 Jul 9.

Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.

The CRISPR/Cas9 system has become a great tool for target gene knock-out in filamentous fungi. It is laborious and time-consuming that identification mutants from a large number of transformants through PCR or enzyme-cut method. Here, we first developed a CRISPR/Cas9 system in Aspergillus oryzae using AMA1-based autonomously replicating plasmid and Cas9 under the control of the Aspergillus nidulans gpdA promoter. By the genome editing technique, we successfully obtained mutations within each target gene in Aspergillus oryzae. Then, we put the protospacer sequence of a target gene and its protospacer adjacent motif (PAM) behind the start codon "ATG" of DsRed, yielding the non‑functional DsRed (nDsRed) reporter gene, and the nDsRed reporter gene could be rescued after successful targeted editing. Moreover, this method was also applied by targeting the kojic acid synthesis gene kojA, and the transformants with DsRed activity were found to harbor targeted mutations in kojA. These results suggest that the nDsRed can be used as a powerful tool to facilitate the identification of mutants generated by CRISPR/Cas9 in Aspergillus oryzae.
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http://dx.doi.org/10.1007/s11274-021-03100-8DOI Listing
July 2021

Research progress in the application of bile acid-drug conjugates: A "trojan horse" strategy.

Steroids 2021 09 26;173:108879. Epub 2021 Jun 26.

State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address:

Bile acid transporters are highly expressed in intestinal cells and hepatocytes, and they determine the uptake of drugs in cells by modulating cellular entry and exit. In order to improve the oral bioavailability of drugs and investigate the potential application prospects of drugs used to target cancer, numerous studies have adopted these transporters to identify prodrug strategies. Through the connection of covalent bonds between drugs and bile acids, the resulting bile acid-drug conjugates continue to be recognized as similar to natural unmodified bile acid and is translocated by the transporter. The present mini-review provides a brief summary of recent progress of the application of bile acid-drug conjugates based primarily on ASBT, NTCP, and OATP, with the hope of contributing to subsequent research.
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http://dx.doi.org/10.1016/j.steroids.2021.108879DOI Listing
September 2021

Somatic variations led to the selection of acidic and acidless orange cultivars.

Nat Plants 2021 07 17;7(7):954-965. Epub 2021 Jun 17.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, P. R. China.

Somatic variations are a major source of genetic diversification in asexual plants, and underpin clonal evolution and the breeding of asexual crops. Sweet orange is a model species for studying somatic variation because it reproduces asexually through apomixis and is propagated asexually through grafting. To dissect the genomic basis of somatic variation, we de novo assembled a reference genome of sweet orange with an average of three gaps per chromosome and a N50 contig of 24.2 Mb, as well as six diploid genomes of somatic mutants of sweet oranges. We then sequenced 114 somatic mutants with an average genome coverage of 41×. Categorization of the somatic variations yielded insights into the single-nucleotide somatic mutations, structural variations and transposable element (TE) transpositions. We detected 877 TE insertions, and found TE insertions in the transporter or its regulatory genes associated with variation in fruit acidity. Comparative genomic analysis of sweet oranges from three diversity centres supported a dispersal from South China to the Mediterranean region and to the Americas. This study provides a global view on the somatic variations, the diversification and dispersal history of sweet orange and a set of candidate genes that will be useful for improving fruit taste and flavour.
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http://dx.doi.org/10.1038/s41477-021-00941-xDOI Listing
July 2021

Comparative Analysis of MicroRNA Expression Profiles Between Skeletal Muscle- and Adipose-Derived Exosomes in Pig.

Front Genet 2021 31;12:631230. Epub 2021 May 31.

Guangdong Province Key Laboratory of Animal Nutritional Regulation, College of Animal Science, South China Agricultural University, Guangzhou, China.

Skeletal muscle and adipose tissues are both involved in regulation of metabolism. In the skeletal muscle-adipose tissue crosstalk, exosomes may play an important role but the main components of exosomes are not clear. In this study, we found skeletal muscle-derived exosomes can inhibit adipogenesis of porcine preadipocytes. We identified microRNA expression profiles of muscle exosomes and adipose exosomes by high-throughput sequencing. There were 104 (both novel and known microRNAs) microRNAs differentially expressed (DE miRNAs) between M-EXO (muscle-derived exosomes) and A-EXO (adipose-derived exosomes) groups. A total of 2,137 target genes of DE miRNAs for M-EXO and 2,004 target genes of DE miRNAs for A-EXO were detected. Bioinformatic analyses revealed that some DE miRNAs of M-EXO (especially miR-221-5p) were mainly enriched in lipid-related metabolism processes. The findings may serve as a fundamental resource for understanding the detailed functions of exosomes between the skeletal muscle-adipose crosstalk and the potential relationship between skeletal muscle atrophy and obesity.
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http://dx.doi.org/10.3389/fgene.2021.631230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202525PMC
May 2021

The GRAS gene family in watermelons: identification, characterization and expression analysis of different tissues and root-knot nematode infestations.

PeerJ 2021 26;9:e11526. Epub 2021 May 26.

College of Life Sciences, Jiangxi Science & Technology Normal University, Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, Nanchang, Jiangxi, China.

The family of GRAS plant-specific transcription factor plays diverse roles in numerous biological processes. Despite the identification and characterization of GRAS genes family in dozens of plant species, until now, GRAS members in watermelon () have not been investigated comprehensively. In this study, using bioinformatic analysis, we identified 37 GRAS genes in the watermelon genome (ClGRAS). These genes are classified into 10 distinct subfamilies based on previous research, and unevenly distributed on 11 chromosomes. Furthermore, a complete analysis was conducted to characterize conserved motifs and gene structures, which revealed the members within same subfamily that have analogous conserved gene structure and motif composition. Additionally, the expression pattern of ClGRAS genes was characterized in fruit flesh and rind tissues during watermelon fruit development and under red light (RL) as well as root knot nematode infestation. Finally, for verification of the availability of public transcriptome data, we also evaluated the expression levels of randomly selected four ClGRAS genes under RL and nematode infection by using qRT-PCR. The qRT-PCR results indicated that several ClGRAS genes were differentially expressed, implying their vital role in RL induction of watermelon resistance against root-knot nematodes. The results obtained in this study could be useful in improving the quality of watermelon.
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http://dx.doi.org/10.7717/peerj.11526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164414PMC
May 2021

Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR.

Animals (Basel) 2021 May 24;11(6). Epub 2021 May 24.

National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

Secretory immunoglobulin A (SIgA) plays an important role in gut acquired immunity and mucosal homeostasis. Breast milk is the irreplaceable nutritional source for mammals after birth. Current studies have shown the potential functional role of milk-derived small extracellular vesicles (sEVs) and their RNAs cargo in intestinal health and immune regulation. However, there is a lack of studies to demonstrate how milk-derived sEVs affect intestinal immunity in recipient. In this study, through in vivo experiments, we found that porcine milk small extracellular vesicles (PM-sEVs) promoted intestinal SIgA levels, and increased the expression levels of polymeric immunoglobulin receptor (pIgR) both in mice and piglet. We examined the mechanism of how PM-sEVs increased the expression level of pIgR in vitro by using a porcine small intestine epithelial cell line (IPEC-J2). Through bioinformatics analysis, dual-luciferase reporter assays, and overexpression or knockdown of the corresponding non-coding RNAs, we identified circ-XPO4 in PM-sEVs as a crucial circRNA, which leads to the expression of pIgR via the suppression of miR-221-5p in intestinal cells. Importantly, we also observed that oral administration of PM-sEVs increased the level of circ-XPO4 and decreased the level of miR-221-5p in small intestine of piglets, indicating that circRNAs in milk-derived sEVs act as sponge for miRNAs in recipients. This study, for the first time, reveals that PM-sEVs have a capacity to stimulate intestinal SIgA production by delivering circRNAs to receptors and sponging the recipient's original miRNAs, and also provides valuable data for insight into the role and mechanism of animal milk sEVs in intestinal immunity.
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http://dx.doi.org/10.3390/ani11061522DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225040PMC
May 2021

Seminar-case learning model improves clinical teaching: a prospective randomized controlled trial.

PeerJ 2021 20;9:e11487. Epub 2021 May 20.

Department of Gastroenterology, The First Affiliated Hospital of University of South China, Hengyang, Hunan, China.

Purpose: The purpose of this research was to assess whether the efficacy of the seminar-case learning model is superior to the traditional lecture-based learning model in the gastroenterology curriculum for first-year graduate students.

Materials & Methods: This research was a prospective randomized controlled trial that enrolled 92 first-year postgraduate students with a rotation internship in the gastroenterology department. The students were randomly divided into 2 groups and then subjected to an identical version of the curriculum for 8 weeks. The experimental group ( = 50) used the seminar-case learning model, while the control group ( = 42) used the traditional lecture-based learning model. Examinations consisted of a theoretical test and a case analysis test, and anonymous questionnaires were used to assess teaching quality.

Results: All participants completed the examinations and questionnaires. The average theoretical test score of the experimental group was no statistical significance with that of the control group ( = 0.17). The average case analysis test score of the experimental group was significantly higher than that of the control group ( < 0.05). The indicators of the experimental group's feedback were better than those of the control group, such that there were significantly higher learning interest and motivation, a better understanding of diseases and knowledge, improvements in clinical thinking and summary ability, and an active classroom atmosphere in the experimental group ( < 0.05). However, students in the experimental group felt more burdensome.

Conclusion: Compared to the traditional method, the seminar-case learning model showed a higher efficacy. The seminar-case learning model effectively improved students' outcomes and satisfaction, which helped students narrow the gap between theoretical knowledge and clinical practical application.
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http://dx.doi.org/10.7717/peerj.11487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141283PMC
May 2021

The mitochondrial fission factor FIS1 promotes stemness of human lung cancer stem cells via mitophagy.

FEBS Open Bio 2021 Jul 19;11(7):1997-2007. Epub 2021 Jun 19.

State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, School of Biomedical Engineering, Chongqing Medical University, China.

Mitophagy, a form of autophagy, plays a role in cancer development, progression and recurrence. Cancer stem cells (CSCs) also play a key role in these processes, although it not known whether mitophagy can regulate the stemness of CSCs. Here, we employed the A549-SD human non-small cell lung adenocarcinoma CSC model that we have developed and characterized to investigate the effect of mitophagy on the stemness of CSCs. We observed a positive relationship between mitophagic activity and the stemness of lung CSCs. At the mechanistic level, our results suggest that augmentation of mitophagy in lung CSCs can be induced by FIS1 through mitochondrial fission. In addition, we assessed the clinical relevance of FIS1 in lung adenocarcinoma using The Cancer Genome Atlas database. An elevation in FIS1, when observed together with other prognostic markers for lung cancer progression, was found to correlate with shorter overall survival.
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http://dx.doi.org/10.1002/2211-5463.13207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406485PMC
July 2021

The transcriptomic response to heat stress of a jujube (Ziziphus jujuba Mill.) cultivar is featured with changed expression of long noncoding RNAs.

PLoS One 2021 27;16(5):e0249663. Epub 2021 May 27.

Institute of Horticulture crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China.

Long non-coding RNA (lncRNA) of plant species undergoes dynamic regulation and acts in developmental and stress regulation. Presently, there is little information regarding the identification of lncRNAs in jujube (Ziziphus jujuba Mill.), and it is uncertain whether the lncRNAs could respond to heat stress (HS) or not. In our previous study, a cultivar (Hqing1-HR) of Z. jujuba were treated by HS (45°C) for 0, 1, 3, 5 and 7 days, and it was found that HS globally changed the gene expression by RNA sequencing (RNA-seq) experiments and informatics analyses. In the current study, 8260 lncRNAs were identified successfully from the previous RNA-seq data, and it indicated that lncRNAs expression was also altered globally, suggesting that the lncRNAs might play vital roles in response to HS. Furthermore, bioinformatics analyses of potential target mRNAs of lncRNAs with cis-acting mechanism were performed, and it showed that multiple differentially expressed (DE) mRNAs co-located with DElncRNAs were highly enriched in pathways associated with response to stress and regulation of metabolic process. Taken together, these findings not only provide a comprehensive identification of lncRNAs but also useful clues for molecular mechanism response to HS in jujube.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249663PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158912PMC
October 2021

Infrared photodissociation spectroscopy of heteronuclear group 15 metal-iron carbonyl cluster anions AFe(CO) (A = Sb, Bi; m, n = 2, 3).

Phys Chem Chem Phys 2021 Jun 26;23(22):12668-12678. Epub 2021 May 26.

School of Chemistry, Biological and Materials Sciences, East China University of Technology, Nanchang, Jiangxi Province 330013, China.

Heteronuclear group 15 metal-iron carbonyl cluster complexes of AFe(CO) (A = Sb, Bi; m, n = 2-3) were generated in the gas phase and studied by infrared photodissociation spectroscopy in the carbonyl stretching region. Their structures were determined by comparing the experimental spectra with predicted spectra derived from DFT calculations at the B3LYP and BP86 levels. All of the AFe(CO) cluster anions were determined to have Fe(CO) fragments with all of the CO ligands terminally bonded to the iron center, and they can be regarded as being formed via the interactions of the neutral group 15 metal clusters with the Fe(CO) fragments. Bonding analyses indicated that each AFe(CO) (n = 2, 3) cluster anion contained two A-Fe single bonds and one A-A double bond. Each AFe(CO) (n = 2, 3) cluster anion involved three A-Fe single bonds and three A-A single bonds. There is an isolobal relationship between the Fe(CO) group and the group 15 atoms. The substitution of an Fe(CO) group in place of one A atom in the tetrahedral A molecule resulted in an AFe(CO) cluster anion with the closed-shell electronic configuration for all the group 15 metals and iron atoms.
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http://dx.doi.org/10.1039/d1cp00583aDOI Listing
June 2021

Data mining of an acoustic biomarker in tongue cancers and its clinical validation.

Cancer Med 2021 06 2;10(11):3822-3835. Epub 2021 May 2.

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.

The promise of speech disorders as biomarkers in clinical examination has been identified in a broad spectrum of neurodegenerative diseases. However, to the best of our knowledge, a validated acoustic marker with established discriminative and evaluative properties has not yet been developed for oral tongue cancers. Here we cross-sectionally collected a screening dataset that included acoustic parameters extracted from 3 sustained vowels /ɑ/, /i/, /u/ and binary perceptual outcomes from 12 consonant-vowel syllables. We used a support vector machine with linear kernel function within this dataset to identify the formant centralization ratio (FCR) as a dominant predictor of different perceptual outcomes across gender and syllable. The Acoustic analysis, Perceptual evaluation and Quality of Life assessment (APeQoL) was used to validate the FCR in 33 patients with primary resectable oral tongue cancers. Measurements were taken before (pre-op) and four to six weeks after (post-op) surgery. The speech handicap index (SHI), a speech-specific questionnaire, was also administrated at these time points. Pre-op correlation analysis within the APeQoL revealed overall consistency and a strong correlation between FCR and SHI scores. FCRs also increased significantly with increasing T classification pre-operatively, especially for women. Longitudinally, the main effects of T classification, the extent of resection, and their interaction effects with time (pre-op vs. post-op) on FCRs were all significant. For pre-operative FCR, after merging the two datasets, a cut-off value of 0.970 produced an AUC of 0.861 (95% confidence interval: 0.785-0.938) for T patients. In sum, this study determined that FCR is an acoustic marker with the potential to detect disease and related speech function in oral tongue cancers. These are preliminary findings that need to be replicated in longitudinal studies and/or larger cohorts.
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http://dx.doi.org/10.1002/cam4.3872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178493PMC
June 2021

Genome-wide identification of the GATA transcription factor family and their expression patterns under temperature and salt stress in Aspergillus oryzae.

AMB Express 2021 Apr 19;11(1):56. Epub 2021 Apr 19.

Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.

GATA transcription factors (TFs) are involved in the regulation of growth processes and various environmental stresses. Although GATA TFs involved in abiotic stress in plants and some fungi have been analyzed, information regarding GATA TFs in Aspergillus oryzae is extremely poor. In this study, we identified and functionally characterized seven GATA proteins from A. oryzae 3.042 genome, including a novel AoSnf5 GATA TF with 20-residue between the Cys-X-Cys motifs which was found in Aspergillus GATA TFs for the first time. Phylogenetic analysis indicated that these seven A. oryzae GATA TFs could be classified into six subgroups. Analysis of conserved motifs demonstrated that Aspergillus GATA TFs with similar motif compositions clustered in one subgroup, suggesting that they might possess similar genetic functions, further confirming the accuracy of the phylogenetic relationship. Furthermore, the expression patterns of seven A. oryzae GATA TFs under temperature and salt stresses indicated that A. oryzae GATA TFs were mainly responsive to high temperature and high salt stress. The protein-protein interaction network of A. oryzae GATA TFs revealed certain potentially interacting proteins. The comprehensive analysis of A. oryzae GATA TFs will be beneficial for understanding their biological function and evolutionary features and provide an important starting point to further understand the role of GATA TFs in the regulation of distinct environmental conditions in A. oryzae.
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http://dx.doi.org/10.1186/s13568-021-01212-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055810PMC
April 2021

Development and validation of multiparametric MRI-based nomogram for predicting occult metastasis risk in early tongue squamous cell carcinoma.

BMC Cancer 2021 Apr 15;21(1):408. Epub 2021 Apr 15.

Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 West Lingyuan Road, Guangzhou, 510055, Guangdong, China.

Background: Nomograms are currently used in predicting individualized outcomes in clinical oncology of several cancers. However, nomograms for evaluating occult nodal metastasis of patients with squamous cell carcinoma of lateral tongue (SCCLT) have not been widely investigated for their functionality. This retrospective cohort study was designed to address this question.

Methods: This study was divided into primary and validation cohorts. The primary cohort comprised 120 patients diagnosed between 2012 and 2017, whereas the validation cohort included 41 patients diagnosed thereafter. The diagnostic value of multiparametric MRI, including radiologic tumor thickness threshold (rTTT) in three-dimensions, paralingual distance, and sublingual distance were investigated. A nomogram was developed based on stepwise logistic regression of potential predictors associated with nodal metastasis in the primary cohort and then tested for predictive accuracy in the validation cohort using area under the curve (AUC) and goodness-of-fit tests.

Results: Multivariate analysis, tumor size (odd ratio [OR] 15.175, 95% confidence interval [CI] 1.436-160.329, P = 0.024), rTTT (OR 11.528, 95% CI 2.483-53.530, P = 0.002), paralingual distance (OR 11.976, 95% CI 1.981-72.413, P = 0.005), and tumor location (OR 6.311, 95% CI 1.514-26.304, P = 0.011) were included in the nomogram to predict the likelihood of having cervical metastasis. A nomogram cutoff value of 210 points (sensitivity 93.8%, specificity 87.5%) was significantly different to classify the patients metastasis risk group (P < 0.001). Nomogram showed predictive accuracy with AUC 0.881 (95% CI 0.779-0.983, P < 0.001) and good calibration after the validation.

Conclusions: A preoperative nomogram incorporating multiparametric MRI demonstrated good prediction and performed adequately in our study. Three-dimensional assessment of occult metastasis risk value obtained from this nomogram can assist in preoperative decision making for individual patients with early-stage SCCLT. The probability of nodal metastasis tended to be greater than 20% in patients with high metastasis risk or nomogram total score > 210 points.
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http://dx.doi.org/10.1186/s12885-021-08135-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048044PMC
April 2021

Identification of NY-ESO-1 Specific Murine T Cell Receptors With Distinct Recognition Pattern for Tumor Immunotherapy.

Front Immunol 2021 23;12:644520. Epub 2021 Mar 23.

Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.

New York esophageal squamous cell carcinoma 1 (NY-ESO-1) is a promising target for T-cell receptor-engineered T cell (TCR-T) therapy, and targeting the human leukocyte antigen (HLA)-A2 restricted NY-ESO-1 epitope has yielded remarkable clinical benefits in the treatment of multiple advanced malignancies. Herein, we report the identification of two NY-ESO-1 epitope-specific murine TCRs obtained from HLA-A0201 transgenic mice. NY-ESO-1 specific TCRs were isolated after vaccinating HLA-A2 transgenic mice with epitope peptides. HZ6 and HZ8 TCRs could specifically bind to NY-ESO-1/HLA-A2 and were capable of cytokine secretion with engineered Jurkat T cells and primary T cells upon recognition with K562 target cells expressing the single-chain trimer (SCT) of NY-ESO-1/HLA-A2. The reactivity profiles of the HZ6 and HZ8 TCRs were found to be distinct from one another when co-cultured with K562 target cells carrying alanine-substituted NY-ESO-1 SCTs. The binding characterization revealed that the recognition pattern of the HZ6 TCR to NY-ESO-1/HLA-A2 was substantially different from the widely used 1G4 TCR. These findings would broaden the understanding of immunogenicity of the NY-ESO-1, and the two identified TCRs may serve as promising candidates for the future development of TCR-T therapy for tumors.
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http://dx.doi.org/10.3389/fimmu.2021.644520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021954PMC
September 2021

Fabrication of Addressable Perovskite Film Arrays for High-Performance Photodetection and Real-Time Image Sensing Application.

J Phys Chem Lett 2021 Mar 16;12(11):2930-2936. Epub 2021 Mar 16.

School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei, Anhui 230009, P. R. China.

Patterned growth of periodic perovskite film arrays is essential for application in sensing devices and integrated optoelectronic systems. Herein, we report on patterned growth of addressable perovskite photodetector arrays through an uncured polydimethylsiloxane (PDMS) oligomer-assisted solution-processed approach, in which a periodic hydrophilic/hydrophobic substrate replicating the predesigned patterns of the PDMS stamp was formed due to the migration of uncured siloxane oligomers in the PDMS stamp to the intimately contacted substrate. By using this technique, MAPbI film photodetector arrays with neglectable pixel-to-pixel variation, a responsivity of 2.83 A W, specific detectivity of 5.4 × 10 Jones, and fast response speed of 52.7/57.1 μs (response/recovery time) were achieved. An 8 × 8 addressable photodetector array was further fabricated, which functioned well as a real-time image sensor with reasonable spatial resolution. It is believed that the proposed strategy will find potential application in large-scale fabrication of other photodetector arrays, which might be potentially important for future integrated optoelectronic devices.
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http://dx.doi.org/10.1021/acs.jpclett.1c00521DOI Listing
March 2021

Cardiomyocyte protective effects of thyroid hormone during hypoxia/reoxygenation injury through activating of IGF-1-mediated PI3K/Akt signalling.

J Cell Mol Med 2021 04 16;25(7):3205-3215. Epub 2021 Mar 16.

Hubei Key Laboratory of Cardiology, Department of Cardiology, Cardiovascular Research Institute, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.

Ischaemia/reperfusion (I/R) injury is a common clinical condition that results in apoptosis and oxidative stress injury. Thyroid hormone was previously reported to elicit cardiac myocyte hypertrophy and promote cardiac function after cardiac injury. We used an in vivo mouse model of I/R injury and in vitro primary cardiomyocyte culture assays to investigate the effects of thyroid hormone on cardiomyocytes during hypoxia/reoxygenation (H/R) injury. The results showed that T3 pretreatment in vivo significantly improved left ventricular function after I/R injury. In vitro, T3 pretreatment decreased cell apoptosis rate, inhibited caspase-3 activity and decreased the Bax/Bcl-2 ration induced by H/R injury. T3 pretreatment significantly attenuated the loss of mitochondrial membrane potential. Furthermore, it was observed that T3 diminished the expression of NCX1 protein and decreased SERCA2a protein expression in H/R-induced cardiomyocytes, and T3 prevented intracellular Ca increase during H/R injury. Also, T3 increased the expression of IGF-1, and PI3K/Akt signalling in cardiomyocytes under H/R-induced injury, and that the protective effect of T3 against H/R-induced injury was blocked by the PI3K inhibitor LY294002. IGF-1 receptor (IGF-1R) inhibitor GSK1904529A significantly inhibited the expression of IGF-1R and PI3K/Akt signalling. In summary, T3 pretreatment protects cardiomyocytes against H/R-induced injury by activating the IGF-1-mediated PI3K/Akt signalling pathway.
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http://dx.doi.org/10.1111/jcmm.16389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034470PMC
April 2021

Resecting a solitary cervical vagal nerve neurofibroma via endoscopic surgery: a case report.

Gland Surg 2021 Feb;10(2):844-851

Department of Breast and Thyroid Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Neurofibromas predominately consist of Schwann cells and fibroblasts, which mainly originate from neurofibromatosis type 1. However, solitary cervical vagal nerve neurofibroma (VNN) has rarely been reported in previously published literature. Additionally, the onset of VNN is characteristically chronic, asymptomatic, and insidious, and is often discovered unexpectedly through physical examination or imaging. The exact etiology and pathogenesis of VNN are yet to be clarified and need further exploration. Consequently, the definitive diagnosis of VNN mainly depends on pathological and immunohistochemical examinations. Immunohistochemically, tumor cells are positive for transcription factor S-100, SRY-related HMG-box (SOX)-10, and vascular marker CD34 will contribute to the diagnosis of VNN. In this uncommon case of left cervical VNN, the patient received comprehensive radiological evaluation before the operation and then underwent mass resection through endoscopic surgery via an axillary and chest wall approach. The patient was satisfied with the postoperative appearance of the neck. Besides, no postoperative complications or recurrence were observed during the consecutive six-month follow-up. Therefore, the successful application of total endoscopic surgery via the bilateral axilla-breast approach (BABA) on this case of neurogenic tumor presents new insights into expanding the operative indications of this technique, which could be a rational candidate for this kind of neck tumor with the requirements of satisfactory aesthetic appearance.
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http://dx.doi.org/10.21037/gs-20-706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944062PMC
February 2021

Effects of the interfacial defects in Au/ TiO on plasmon-induced water oxidation.

J Chem Phys 2020 May;152(19):194702

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian 116023, China.

In the plasmonic metal-semiconductor systems, the interfacial structure is vital for both charge separation and photocatalytic reaction. However, the role of interfacial defects, a ubiquitous phenomenon in the metal-semiconductor heterostructure, is not well understood, especially for the hot hole-involved water oxidation reaction. Herein, we studied the effect of interfacial defects, derived from oxygen vacancies, on plasmonic photocatalytic water oxidation. In addition, we found that the plasmon-induced water oxidation activity decreased with the increase in the oxygen vacancies present at the interface, and the activity of Au/TiO can be restored after eliminating the defects via a post-oxidation treatment. It is elucidated that a defect state appeared below the conduction band of TiO as a result of interfacial defects, which acts as the electron traps and backward transfer channel for electrons to combine with the holes left at the interface. The charge recombination at defect sites leads to the shorter lifetime of hot holes, which is harmful for the kinetics-sluggish water oxidation. This work emphasizes the significance of the interface structure for the plasmon-based photocatalytic process.
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http://dx.doi.org/10.1063/5.0008382DOI Listing
May 2020
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