Publications by authors named "Bin Shan"

130 Publications

Inherently Area-Selective Atomic Layer Deposition of Manganese Oxide through Electronegativity-Induced Adsorption.

Molecules 2021 May 20;26(10). Epub 2021 May 20.

State Key Laboratory of Digital Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430063, China.

Manganese oxide (MnO) shows great potential in the areas of nano-electronics, magnetic devices and so on. Since the characteristics of precise thickness control at the atomic level and self-align lateral patterning, area-selective deposition (ASD) of the MnO films can be used in some key steps of nanomanufacturing. In this work, MnO films are deposited on Pt, Cu and SiO substrates using Mn(EtCp) and HO over a temperature range of 80-215 °C. Inherently area-selective atomic layer deposition (ALD) of MnO is successfully achieved on metal/SiO patterns. The selectivity improves with increasing deposition temperature within the ALD window. Moreover, it is demonstrated that with the decrease of electronegativity differences between M (M = Si, Cu and Pt) and O, the chemisorption energy barrier decreases, which affects the initial nucleation rate. The inherent ASD aroused by the electronegativity differences shows a possible method for further development and prediction of ASD processes.
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http://dx.doi.org/10.3390/molecules26103056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161048PMC
May 2021

Synergetic effect dependence on activated oxygen in the interface of NiO-modified Pt nanoparticles for the CO oxidation from first-principles.

Phys Chem Chem Phys 2021 Apr 26;23(14):8541-8548. Epub 2021 Mar 26.

Department of Physics and Institute of Condensed Matter Physics, School of Science, Wuhan University of Technology, Wuhan 430070, China.

CO oxidation on NiO-modified Pt nanoparticles (NPs) was investigated by first-principles calculations and microkinetic methods. The binding energies of O and CO on NiO/Pt suggest that CO adsorption is dominant and the CO oxidation mainly follows the Mars-van Krevelen (M-vK) mechanism. It was found that the interfacial O of NiO/Pt played a key role in the combination of adsorbed CO to O, as well as the O dissociation. With a lower O vacancy formation energy, NiO/Pt shows about four orders higher reaction rates than NiO/Pt. Microkinetic analysis suggests that the rate-determining step also depends on the active O at the interface. The calculations highlight the synergetic effect difference of NiO selectively deposited on the different sites of Pt NPs on the CO oxidation from the atomic reaction mechanism, and throws light on the high activity of CO oxidation on partially covered NiO/Pt nanoparticles.
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http://dx.doi.org/10.1039/d1cp00149cDOI Listing
April 2021

A modified rotating isosceles triangle osteotomy using a 3D-printed patient-specific guide for the treatment of cubitus varus in children: a case report and literature review.

Transl Pediatr 2021 Jan;10(1):215-222

Department of Orthopaedic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

After corrective osteotomy of cubitus varus, the lateral condylar prominence is a common problem, which is believed to be due to the unequal relative cuts of the lateral base wedge osteotomy. Therefore, several related solutions have been proposed, such as dome osteotomy and step-cut osteotomies, which solve the above problems to a certain extent. This study aimed to: (I) use a modified corpectomy to correct the deformity, and (II) present a new corpectomy method that uses a 3D-printed specific guide with an isosceles triangle osteotomy. A 12-year-old male presented with a -30-degree cubitus varus deformity 5 years after a supracondylar fracture of the right humerus. The degree of correction was determined from the varus angle and the normal carrying angle on the normal side. A rotating isosceles triangle osteotomy was determined by using Mimics software. The accuracy of the osteotomy angle was confirmed by postoperative radiography. The mean postoperative carrying angle was found to be preserved at the 10-month follow-up, with no complications. A rotating isosceles triangle osteotomy with a 3D-printed patient-specific guide may be providing a relative accurate result. However, in order to obtain more rigorous research conclusions, more cases should be added to examine this methodology for bone deformity surgery in the near future.
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http://dx.doi.org/10.21037/tp-20-101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882299PMC
January 2021

A graphical guide for constructing a finite element model of the cervical spine with digital orthopedic software.

Ann Transl Med 2021 Jan;9(2):169

Department of Orthopaedic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Three-dimensional (3D) reconstruction and finite element analysis (FEA) have been extensively used to simulate cervical biomechanics. However, instructive articles providing full descriptions for operating Mimics software, Geomagic software, and FEA are rare in the literature. This omission has hindered research and development related to cervical spine biomechanics. Herein, we expound a detailed and easily understandable protocol for performing a digital biomechanics study which may facilitate a better understanding of the internal anatomy mechanics and the investigation of novel screw fixation techniques. We describe step-by-step instructions for use of Mimics and Geomagic software in FEA, along with a concise literature review. The key procedures of digital FEA stepwise instruction are presented, accompanied by a brief but complete report on the computed tomography (CT) imaging data for establishing the final finite element model. Previous publications regarding the commonly used software are also reviewed and discussed. Each piece of software performs a specific function for digital FEA establishment and each has its inherent shortcomings, making it is necessary to combine the software to leverage the advantages of each in order to best serve finite element research. For reasons of brevity, this study only provides an illustrative report on a small key part of finite element research in the cervical spine. These stepwise instructions can guide orthopedic researchers in conducting FEA studies in digital cervical biomechanics.
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http://dx.doi.org/10.21037/atm-20-2451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867904PMC
January 2021

In Vitro Activity of Imipenem/Relebactam Against Enterobacteriaceae Isolates Obtained from Intra-abdominal, Respiratory Tract, and Urinary Tract Infections in China: Study for Monitoring Antimicrobial Resistance Trends (SMART), 2015-2018.

Clin Infect Dis 2020 12;71(Suppl 4):S427-S435

Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing, China.

Background: Considering the increasing incidence of carbapenem-resistant Enterobacteriaceae in China, this study aimed to establish the in vitro effectiveness of imipenem/relebactam (IMI/REL) on clinical Enterobacteriaceae isolates derived from intra-abdominal infections (IAIs), respiratory tract infections (RTIs), and urinary tract infections (UTIs) in China between 2015 and 2018.

Methods: In total, 8781 Enterobacteriaceae isolates from IAI, RTI, and UTI samples were collected from 22 hospitals across 7 geographic regions of China. Susceptibility to antimicrobial drugs was tested using the Clinical and Laboratory Standards Institute broth microdilution and breakpoints, and IMI/REL activity was assessed using United States Food and Drug Administration guidelines.

Results: In 2015-2018, the most frequently identified Enterobacteriaceae species was Escherichia coli (n = 4676 [53.3%]), followed by Klebsiella pneumoniae (n = 2949 [33.6%]) and Enterobacter cloacae (n = 542 [6.2%]). The Enterobacteriaceae isolates showed 95.2% overall susceptibility to IMI/REL, of which the susceptibility rates in isolates from IAI, RTI, and UTI were 95.8%, 91.4%, and 96.6%, respectively. Overall, the susceptibilities of both intensive care unit (ICU) and non-ICU Enterobacteriaceae isolates to colistin were 92.9%, followed by IMI/REL (90.7% [95.9%]) and amikacin (83.3% [92.3%]). In addition, IMI/REL restored 66.3% susceptibility in imipenem-nonsusceptible Enterobacteriaceae.

Conclusions: Given their high in vitro susceptibility, Enterobacteriaceae infections in China should be considered for IMI/REL treatment, especially with isolates that are not susceptible to carbapenems.
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http://dx.doi.org/10.1093/cid/ciaa1519DOI Listing
December 2020

Polymyxin resistance in carbapenem-resistant Enterobacteriaceae isolates from patients without polymyxin exposure: a multicentre study in China.

Int J Antimicrob Agents 2021 Feb 23;57(2):106262. Epub 2020 Dec 23.

(a)Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. Electronic address:

Polymyxins were recently approved for the clinical treatment of carbapenem-resistant Enterobacteriaceae (CRE) infections in China. The aim of this study was to determine the prevalence and molecular mechanisms of polymyxin-resistant CRE prior to the clinical application of polymyxin and to evaluate the potential for emerging polymyxin resistance in China. A total of 504 unique CRE isolates were collected from six tertiary-care hospitals in China between October 2016 and September 2017. All isolates underwent antimicrobial susceptibility testing. Clinical, demographic, antimicrobial exposure and infection data were collected from patients' medical charts. PCR detection, Sanger sequencing and reverse transcription real-time fluorescence quantitative PCR (RT-qPCR) were used to investigate the molecular mechanism of polymyxin resistance. A total 19 (3.8%) polymyxin-resistant isolates were identified, including Klebsiella pneumoniae, Escherichia coli, Klebsiella aerogenes and Enterobacter cloacae. Genetic analysis in K. pneumoniae strains identified insertion sequence (IS) elements (n = 3), a stop codon (n = 1) and gene deletion (n = 2) in mgrB and a pmrB missense mutation (T157P) (n = 1). Two E. coli isolates contained mcr-1 and an E. cloacae strain harboured a frameshift in mgrB. Further transcriptional analysis showed that pmrA, pmrB, pmrC and pmrK were significantly upregulated in polymyxin-resistant isolates. Despite the lack of polymyxin exposure, 3.8% of CRE were resistant to polymyxin in China. Both chromosomal and plasmid-encoded mechanisms were identified. Our study suggests that clinical practice should be alert to pre-existing polymyxin resistance among CRE isolates to avoid further dissemination of polymyxin resistance.
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http://dx.doi.org/10.1016/j.ijantimicag.2020.106262DOI Listing
February 2021

Author Correction: Activation of subnanometric Pt on Cu-modified CeO via redox-coupled atomic layer deposition for CO oxidation.

Nat Commun 2020 Nov 12;11(1):5879. Epub 2020 Nov 12.

State Key Laboratory of Digital Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, People's Republic of China.

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-19663-3 .
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http://dx.doi.org/10.1038/s41467-020-19663-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665035PMC
November 2020

LncRNA H19 Inhibits the Progression of Sepsis-Induced Myocardial Injury via Regulation of the miR-93-5p/SORBS2 Axis.

Inflammation 2021 Feb 29;44(1):344-357. Epub 2020 Sep 29.

Department of Critical Care Medicine, The First People's Hospital of Chenzhou, No. 8, Qinnian Avenue Road, Chenzhou, 423000, Hunan Province, People's Republic of China.

Sepsis is an infectious disease that seriously endangers human health. It usually leads to myocardial injury which seriously endangers to the health of human beings. H19 has been confirmed to play key roles in various diseases, including sepsis. However, its function in the progression of sepsis-induced myocardial injury remains largely unknown. H9C2 cells were treated with lipopolysaccharide (LPS) to mimic sepsis-induced myocardial injury in vitro. Cell proliferation and apoptosis were detected by MTT assay and flow cytometry, respectively. In addition, gene and protein expression levels in H9C2 cells were measured by quantitative real-time PCR (qRT-PCR) and Western blotting. The levels of inflammatory cytokines in H9C2 cell supernatants were tested by ELISA. JC-1 staining was performed to observe the mitochondrial membrane potential level in H9C2 cells. H19 and SORBS2 were downregulated in H9C2 cells following LPS treatment, while miR-93-5p was upregulated. Moreover, LPS-induced cell growth inhibition and mitochondrial damage were significantly reversed by overexpression of H19. In addition, H19 upregulation notably suppressed LPS-induced inflammatory responses in H9C2 cells. Moreover, H19 sponged miR-93-5p to promote SORBS2 expression. Overall, H19 suppressed sepsis-induced myocardial injury via regulation of the miR-93-5p/SORBS2 axis. H19 attenuated the development of sepsis-induced myocardial injury in vitro via modulation of the miR-93-5p/SORBS2 axis. Thus, H19 could serve as a potential target for the treatment of sepsis-induced myocardial injury.
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http://dx.doi.org/10.1007/s10753-020-01340-8DOI Listing
February 2021

Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice.

Ann Palliat Med 2020 Sep 14;9(5):3249-3260. Epub 2020 Sep 14.

Qinghai Provincial Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, China.

Background: Ma-Nuo-Xi decoction (MNXD), as well as its hundreds of derivative preparations, has been used in Tibetan medicine since the 14th century. MNXD is in accordance with the theory of treatment determination based on syndrome differentiation. This study aimed to compare the effect of the auxiliary MNXD prescription (MNXD-AD) with that of the basic MNXD prescription (MNXD-BD) on the immunostimulating activity of MNXD.

Methods: The immunopotentiation of MNXD, MNXD-BD, and MNXD-AD was evaluated using a cyclophosphamide (CTX)-immunosuppressed mouse model. Their influences on non-specific and specific immunity were evaluated using immune organ indexes, peripheral white blood cell (WBC) count, red blood cell (RBC) count, platelet count, phagocytosis, macrophage-secreted nitric oxide (NO) and cytokines, natural killer (NK) cytotoxic activity, lymphocyte proliferation, serum cytokines, splenic T-lymphocyte subpopulations, and quantitative hemolysis of sheep red blood cell (QHS SRBC) assays.

Results: MNXD, MNXD-BD, and MNXD-AD increased the spleen and thymus indexes, as well as the peripheral WBC, RBC, and platelet counts. They also promoted phagocytosis, NO and cytokine secretion from macrophages, NK cytotoxic activity, and lymphocyte proliferation, and also raised the CD4+ /CD8+ T-cell ratio, serum cytokine concentrations, and haemolysin formation in CTX-treated immunosuppressed mice. Compared with MNXD-BD and MNXD-AD, MNXD was superior in restoring the phagocytic index, concanavalin A (ConA)-induced T-lymphocyte proliferation, NO secretion from macrophages, and haemolysin formation, as well as the levels of interleukin 1 beta (IL-1β), and serum interleukin-2 (IL-2) and interferon gamma (INF-γ).

Conclusions: MNXD, MNXD-BD, and MNXD-AD have excellent immunostimulating and myelosuppression-restoring activities on CTX-immunosuppressed mice. Among them, MNXD-AD might be an immunomodulator, which may happen to be in line with the clinical experience of Tibetan medicine physicians of using it to promote the efficacy of MNXD-BD.
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http://dx.doi.org/10.21037/apm-20-1492DOI Listing
September 2020

Activation of subnanometric Pt on Cu-modified CeO via redox-coupled atomic layer deposition for CO oxidation.

Nat Commun 2020 Aug 25;11(1):4240. Epub 2020 Aug 25.

State Key Laboratory of Digital Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, 430074, Wuhan, Hubei, People's Republic of China.

Improving the low-temperature activity (below 100 °C) and noble-metal efficiency of automotive exhaust catalysts has been a continuous effort to eliminate cold-start emissions, yet great challenges remain. Here we report a strategy to activate the low-temperature performance of Pt catalysts on Cu-modified CeO supports based on redox-coupled atomic layer deposition. The interfacial reducibility and structure of composite catalysts have been precisely tuned by oxide doping and accurate control of Pt size. Cu-modified CeO-supported Pt sub-nanoclusters demonstrate a remarkable performance with an onset of CO oxidation reactivity below room temperature, which is one order of magnitude more active than atomically-dispersed Pt catalysts. The Cu-O-Ce site with activated lattice oxygen anchors deposited Pt sub-nanoclusters, leading to a moderate CO adsorption strength at the interface that facilitates the low-temperature CO oxidation performance.
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http://dx.doi.org/10.1038/s41467-020-18076-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447628PMC
August 2020

Epigenetic upregulation of in non-small lung cancer cells.

Aging (Albany NY) 2020 Jul 19;12(17):16921-16935. Epub 2020 Jul 19.

Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University Spokane, WA 99202, USA.

The homeobox genes (HOX) have emerged as a new family of master regulators of development and cancer. In the current study, we examined the expression and function of in human non-small cell lung cancer (NSCLC). We observed increased expression of in the more aggressive human NSCLC cell line NCI-H23 over the well differentiated A549 cells. To elucidate the expression and function of in NSCLC cells, we employed RT-PCR, immunoblotting, methylation-specific PCR, apoptosis assays, and xenograft model. Overexpression of in A549 cells conveyed increased proliferation, reduced apoptosis, and accelerated tumor growth when transplanted into nude mice. In contrast, siRNA-mediated knockdown of in NCI-H23 cells reduced proliferation and increased apoptosis. Our results further indicated that hypomethylation of the CpG island in the promoter was critical to elevated expression of in NSCLC cells. Lastly, we identified a G-quadruplex in the promoter and its G-quadruplex formation was required for elevated expression of in NSCLC cells. Moreover our results suggest that disruption of G-quadruplex formation can silence expression in NSCLC cells. In summary, we report as a novel tumor promoting oncogene in NSCLC cells.
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http://dx.doi.org/10.18632/aging.103597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521540PMC
July 2020

Reduced Ceftazidime-Avibactam Susceptibility in KPC-Producing From Patients Without Ceftazidime-Avibactam Use History - A Multicenter Study in China.

Front Microbiol 2020 23;11:1365. Epub 2020 Jun 23.

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

(KPC-KP) is the most widely spread carbapenem-resistant (CRE) in China. Avibactam is a novel non-β-lactam β-lactamase inhibitor which is highly active against KPC. Recently, (CAZ-AVI) was approved for clinical treatment in China. Here we conducted a retrospective study to examine the antimicrobial susceptibility of CAZ-AVI prior to its usage in China, and evaluated the potential to develop resistance in KPC-KP. CAZ-AVI MICs were tested in 347 KPC-KP isolates collected from patients with no prior treatment with this combination from six medical centers in China. Almost all isolates ( = 346; 99.7%) were CAZ-AVI-susceptible, with only 12 (3.5%) which showed reduced susceptibility (MIC ≥ 4/4 μg/ml) or resistance. The 12 isolates belong to ST11 and half of them carry virulence genes. In comparison to susceptible isolates, these isolates demonstrated higher copy numbers and expressions, and demonstrated higher frequency of developing CAZ-AVI resistance.
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http://dx.doi.org/10.3389/fmicb.2020.01365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324628PMC
June 2020

Multicenter Evaluation of the Xpert Carba-R Assay for Detection and Identification of Carbapenemase Genes in Sputum Specimens.

J Clin Microbiol 2020 08 24;58(9). Epub 2020 Aug 24.

China Aviation General Hospital of China Medical University, Beijing, China.

Rapid diagnosis of infections caused by carbapenem-resistant (CRE) is crucial for proper treatment and infection control. The Xpert Carba-R assay is a qualitative multiplex real-time PCR method that qualitatively detects and differentiates five common carbapenemase genes (, , , , and ) directly from rectal swabs or purified colonies within approximately 1 h. We performed a multicenter evaluation of the investigational use of the Carba-R assay for detection and differentiation of carbapenemase genes from sputum specimens in patients with a clinical diagnosis of pneumonia. The intra- and interassay coefficients of variation values for the Carba-R assay were 0.2% to 2.0% and 1.4% to 2.3%, respectively. A total of 301 sputum specimens were collected and tested. Compared to bacterial culture followed by PCR identification of resistance genes from colonies, the Carba-R assay reduced turnaround time from 56 to 84 h to less than 2 h. Carbapenemase genes were detected by the Carba-R assay in ( = 236), ( = 22), ( = 23), ( = 8), ( = 6), ( = 4), and ( = 2). The Carba-R assay detected 112 (33.5%), 70 (21.0%), 8 (2.4%), and 2 (0.6%) genes, with positive percent agreement, negative percent agreement, and concordance rates of 92.9%, 86.7%, and 88.3%, respectively, for the dominant and 85.0%, 87.8%, and 87.4%, respectively, for the genes. Neither method detected the carbapenemase gene. The convenient, rapid, and simple characteristics of the Xpert Carba-R assay make it a potential tool for CRE detection and identification directly in sputum specimens.
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http://dx.doi.org/10.1128/JCM.00644-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448655PMC
August 2020

In vitro activity of aztreonam-avibactam against metallo-β-lactamase-producing Enterobacteriaceae-A multicenter study in China.

Int J Infect Dis 2020 Aug 28;97:11-18. Epub 2020 May 28.

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address:

Objectives: To study the molecular epidemiology of clinical metallo-β-lactamase (MBL)-producing Enterobacteriaceae isolates in China and to evaluate the antimicrobial susceptibility of MBL-Enterobacteriaceae isolates to aztreonam-avibactam.

Methods: Bacterial speciation was determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. PCR was used to screen for common carbapenemase genes. Antimicrobial susceptibility testing of common clinical antibiotics and aztreonam-avibactam was performed using the standard broth microdilution method.

Results: A total of 161 MBL-Enterobacteriaceae isolates were included, with Klebsiella pneumoniae (n = 73, 45.4%) and Escherichia coli (n = 53, 32.9%) being the most common species. Among the 161 isolates, bla (n = 151), bla (n = 13), and bla (n = 2) were detected, including five strains (3.1%) co-harboring two MBLs. MBL-Enterobacteriaceae isolates frequently contained two (n = 55, 34.2%) or more (n = 89, 55.3%) additional serine β-lactamase genes (bla, bla, bla, or bla). Antimicrobial susceptibility testing showed that 81.4% of isolates (n = 131) were resistant to aztreonam. The rates of resistance to cefazolin, ceftazidime, ceftriaxone, cefotaxime, ampicillin-sulbactam, amoxicillin-clavulanic acid, and piperacillin-tazobactam were all over 90%. The addition of avibactam (4 μg/ml) significantly reduced the minimum inhibitory concentrations (MICs) of the aztreonam-resistant isolates by more than 8-fold (range ≤0.125 to 4 μg/ml), with a MIC/MIC of ≤0.125/1 μg/ml among the 131 isolates. Overall, 96.9% (n = 156) of the total isolates were inhibited at an aztreonam-avibactam concentration of ≤1 μg/ml. Univariate and multivariate logistic regression analysis found that in patients with MBL-Enterobacteriaceae infections, the presence of pre-existing lung disease (adjusted odds ratio 8.267, 95% confidence interval 1.925-28.297; p = 0.004) was associated with a hazard effect on worse disease outcomes.

Conclusions: The combined use of aztreonam-avibactam is highly potent against MBL-Enterobacteriaceae and may serve as a new candidate for the treatment of infections caused by MBL-Enterobacteriaceae in China.
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http://dx.doi.org/10.1016/j.ijid.2020.05.075DOI Listing
August 2020

High efficiency and stability of ink-jet printed quantum dot light emitting diodes.

Nat Commun 2020 Apr 2;11(1):1646. Epub 2020 Apr 2.

TCL Research, No. 1001 Zhongshan Park Road, Nanshan District, Shenzhen, 518067, People's Republic of China.

The low efficiency and fast degradation of devices from ink-jet printing process hinders the application of quantum dot light emitting diodes on next generation displays. Passivating the trap states caused by both anion and cation under-coordinated sites on the quantum dot surface with proper ligands for ink-jet printing processing reminds a problem. Here we show, by adapting the idea of dual ionic passivation of quantum dots, ink-jet printed quantum dot light emitting diodes with an external quantum efficiency over 16% and half lifetime of more than 1,721,000 hours were reported for the first time. The liquid phase exchange of ligands fulfills the requirements of ink-jet printing processing for possible mass production. And the performance from ink-jet printed quantum dot light emitting diodes truly opens the gate of quantum dot light emitting diode application for industry.
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http://dx.doi.org/10.1038/s41467-020-15481-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118149PMC
April 2020

Vasculogenic mimicry in carcinogenesis and clinical applications.

J Hematol Oncol 2020 03 14;13(1):19. Epub 2020 Mar 14.

Department of Thoracic Surgery, Xiangya Hospital, Central South University, Xiangya Road 87th, Changsha, 410008, Hunan, People's Republic of China.

Distinct from classical tumor angiogenesis, vasculogenic mimicry (VM) provides a blood supply for tumor cells independent of endothelial cells. VM has two distinct types, namely tubular type and patterned matrix type. VM is associated with high tumor grade, tumor progression, invasion, metastasis, and poor prognosis in patients with malignant tumors. Herein, we discuss the recent studies on the role of VM in tumor progression and the diverse mechanisms and signaling pathways that regulate VM in tumors. Furthermore, we also summarize the latest findings of non-coding RNAs, such as lncRNAs and miRNAs in VM formation. In addition, we review application of molecular imaging technologies in detection of VM in malignant tumors. Increasing evidence suggests that VM is significantly associated with poor overall survival in patients with malignant tumors and could be a potential therapeutic target.
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http://dx.doi.org/10.1186/s13045-020-00858-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071697PMC
March 2020

The influence of adjacent Al atoms on the hydrothermal stability of H-SSZ-13: a first-principles study.

Phys Chem Chem Phys 2020 Feb 17;22(5):2930-2937. Epub 2020 Jan 17.

State Key Laboratory of Material Processing and Die and Mould Technology and School of Materials Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

The Al concentration and distribution have a great influence on the hydrothermal stability of the H-SSZ-13 zeolites in experiments. In this work, first-principles calculations are performed to clarify the decomposition mechanism of an H-SSZ-13 framework with adjacent Al atom pair distribution under hydrothermal conditions. It is found that the adjacent Al atoms have a tendency to occupy the para-sites of the 4-membered rings (4MRs) in the framework. Water molecules are chemisorbed onto the Al atom one by one, and the hydroxylation of the neighboring O atoms induces the breaking of the Al-O bonds, which causes the first dealumination in 4MRs. The other Al atom in the para-site can be easily removed from the framework once the first one is lost. The feasible subsequent dealumination of adjacent Al atoms would break the linker of 6MRs in the framework, which is responsible for the degraded hydrothermal stability. Moreover, the partial substitution of metal ions (such as Na and Cu) for the protons in the framework will greatly stabilize the Al-O bonds and enlarge the energy barrier of para-site Al dealumination, which leads to the improved hydrothermal stability of H-SSZ-13.
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http://dx.doi.org/10.1039/c9cp05141dDOI Listing
February 2020

Inhibition of HDAC6 Attenuates Tumor Growth of Non-Small Cell Lung Cancer.

Transl Oncol 2020 Feb 19;13(2):135-145. Epub 2019 Dec 19.

Tulane University Health Sciences Center, Department of Medicine, New Orleans, LA 70112, USA. Electronic address:

Histone deacetylase 6 (HDAC6) regulates cytoplasmic signaling networks through deacetylation of various cytoplasmic substrates and serves as a key member of the ubiquitin proteasome system (UPS). This study is focused on HDAC6 regulation of the Notch1 receptor that plays a crucial role in tumor growth in NSCLC. A series of cell culture experiments were employed using A549, Lewis lung carcinoma 2 (LL2), and H1299 NSCLC cell lines to investigate HDAC6-mediated regulation of the Notch1 receptor through the UPS. HDAC6 was inhibited with small molecule inhibitors tubacin and ACY1215 in vitro and in vivo. Inhibition of HDAC6 led to reduced levels of Notch1 receptor in a dose-dependent manner in all three NSCLC cell lines tested. HDAC6 inhibition with ACY1215 led to G2 arrest, increased apoptosis, and increased levels of cleaved PARP1 in A549, LL2, and H1299 cell lines. In vivo inhibition of HDAC6 with ACY1215 significantly reduced LL2 tumor growth rate. Our data show that HDAC6 in NSCLC cells supports Notch1 signaling and promotes cell survival and proliferation. Our results support clinical investigation of HDAC6 inhibitors as a potential therapeutic option for treatment of NSCLC patients.
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http://dx.doi.org/10.1016/j.tranon.2019.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926313PMC
February 2020

Recent Progress in Characterizing Long Noncoding RNAs in Cancer Drug Resistance.

J Cancer 2019 22;10(26):6693-6702. Epub 2019 Oct 22.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, PR China.

Drug resistance is an important cause of failure in cancer chemotherapies. A large number of long noncoding RNAs (lncRNAs) have been found to be related to drug resistance in cancers. Therefore, lncRNAs provide potential targets for cancer therapies. The lncRNAs involved in cancer drug resistance are attracting interest from an increasing number of researchers. This review summarizes the latest research on the mechanisms and functions of lncRNAs in cancer drug resistance and envisages their future developments and therapeutic applications. This research suggests that lncRNAs regulate drug resistance through multiple mechanisms. LncRNAs do not affect drug resistance directly; usually, they do so by regulating the expression of some intermediate regulatory factors. In addition, lncRNAs exhibit a diversity of functions in cancer drug resistance. The overexpression of most lncRNAs promotes drug resistance, while a few lncRNAs have inhibitory effects.
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http://dx.doi.org/10.7150/jca.30877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856905PMC
October 2019

Potential role of the antimicrobial peptide against multidrug-resistant and coinfection in an animal model.

Infect Drug Resist 2019 23;12:2865-2874. Epub 2019 Sep 23.

Department of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, People's Republic of China.

Background: , an antimicrobial peptide (AMP), provides protection against multidrug-resistant (MDR) bacterial infections and shows cytotoxicity to mammalian cells. Mixed bacterial infections, of which plus is the most common and dangerous combination, are critical contributors to the morbidity and mortality of long-term in-hospital respiratory medicine patients. Therefore, the development of effective therapeutic approaches to mixed bacterial infections is urgently needed.

Methods And Results: In this study, we demonstrated that compared with individual infections, mixed infections with MDR bacteria and cause more serious diseases, with increased pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and chemokines (MCP-1/MIP-2) and reduced mouse survival. In vitro treatment with enhanced phagocytosis in a mouse alveolar macrophage cell line (MH-S). Strikingly, in vivo, demonstrated a potential role against mixed-MDR bacterial coinfection. The bacterial burden in bronchoalveolar lavage fluid (BALF) was significantly reduced in the treated group. In addition, a systemic reduction in pro-inflammatory cytokines and decreased lung injury occurred with therapy.

Conclusion: Therefore, our study demonstrated that represents a potential therapeutic treatment against mixed-MDR bacterial infection in vivo, which sheds light on the development of therapeutic strategies against mixed-MDR bacterial infections.
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http://dx.doi.org/10.2147/IDR.S217020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765326PMC
September 2019

LINC81507 act as a competing endogenous RNA of miR-199b-5p to facilitate NSCLC proliferation and metastasis via regulating the CAV1/STAT3 pathway.

Cell Death Dis 2019 07 11;10(7):533. Epub 2019 Jul 11.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, PR China.

Lung cancer is the leading cause of cancer-related mortality worldwide. Recently, accumulating data indicate that long noncoding RNAs (LncRNAs) function as novel crucial regulators of diverse biological processes, including proliferation and metastasis, in tumorigenesis. Lnc NONHSAT081507.1 (LINC81507) is associated with lung adenocarcinoma. However, its pathological role in non-small cell lung cancer (NSCLC) remains unknown. In our study we investigated the role of LINC81507 in NSCLC. The expression of LINC81507 was analyzed in 105 paired NSCLC tumor specimens and paired adjacent non-tumorous tissues from NSCLC patients by real-time quantitative PCR (RT-qPCR). Gain- and loss-of-function experiments were conducted to investigate the functions of LINC81507, miR-199b-5p and CAV1. Reduced expression of LINC81507 resulted in cell growth, proliferation, migration and epithelial-mesenchymal transition (EMT) in NSCLC cells, whereas ectopic overexpression of LINC81507 resulted in the opposite effects both in vitro and in vivo. Nuclear and Cytoplasmic fractionation assays showed LINC81507 mainly resided in cytoplasm. Bioinformatics analysis and dual-luciferase assays revealed that miR-199b-5p was a direct target of LINC81507 through binding Ago2. Mechanistic analysis demonstrated that miR-199b-5p specifically targeted the Caveolin1 (CAV1) gene, and LINC81507 inactivated the STAT3 pathway in a CAV1-dependent manner. Taken together, LINC81507 is decreased in NSCLC and functions as a sponge to miR-199b-5p to regulate CAV1/STAT3 pathway, which suggests that LINC81507 serve as a tumor suppressor and potential therapeutic target and biomarker for metastasis and prognosis in NSCLC.
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http://dx.doi.org/10.1038/s41419-019-1740-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6624296PMC
July 2019

MicroRNA-638 expression change in osteosarcoma patients via PLD1 and VEGF expression.

Exp Ther Med 2019 May 22;17(5):3899-3906. Epub 2019 Mar 22.

College of Medical Sciences, Washington State University, Spokane, WA 99201, USA.

The present study aimed to investigate the function and mechanism of microRNA-638 (miR-638) in osteosarcoma. MiR-638 expression change in patients with osteosarcoma was detected by reverse transcription-quantitative polymerase chain reaction. Expression of miR-638 was observed to be downregulated in patients with osteosarcoma compared with the control group. , overexpression of miR-638 induced apoptosis, and inhibited cell proliferation and invasion of osteosarcoma cells. Overexpression of miR-638 induced Bcl-2 associated X and caspase-3 protein expression, and suppressed cyclin D1, phospholipase D1 (PLD1) and vascular endothelial growth factor (VEGF) protein expression in osteosarcoma. The promotion of PLD1 decreased the effects of miR-638 on osteosarcoma cell proliferation. In summary, it was demonstrated that miRNA-638 expression change in patients with osteosarcoma and an model via PLD1 and VEGF expression and miRNA-638 may be potential clinical indicators of osteosarcoma.
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http://dx.doi.org/10.3892/etm.2019.7429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447936PMC
May 2019

Dyeing Method and Properties of a Novel Blue Azo-Anthraquinone Reactive Dye on Cotton.

Molecules 2019 Apr 4;24(7). Epub 2019 Apr 4.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China.

A novel blue azo-anthraquinone reactive dye was evaluated in the dyeing of cotton by using a dip-pad-steam process. Dyeing method and properties were examined in detail and the results showed that the dyeing method consisting of dye concentration of 25 g/L, sodium carbonate of 12 g/L, dipping time of 3 min and steaming time of 30 min was the most effective when a conventional "one-dip-one-nip" process was used. The fixation of the dyes on cotton could reach up to 93.4%, the wash and rub fastness both reached grade 4 above, and the light fastness reached grade 4-5 above. Such colored cotton showed very close colorimetric properties.
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http://dx.doi.org/10.3390/molecules24071334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480443PMC
April 2019

HOX transcript antisense RNA (HOTAIR) in cancer.

Cancer Lett 2019 07 12;454:90-97. Epub 2019 Apr 12.

Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University-Spokane, WA, 99202, USA. Electronic address:

Long noncoding RNAs (lncRNAs) have emerged as a new family of master regulators of cancer. The lncRNA HOX transcript antisense RNA (HOTAIR) is a prime example of an oncogenic trans-acting lncRNA. The expression of HOTAIR is elevated in a broad spectrum of cancers and is associated with metastasis and poor prognosis. HOTAIR governs fundamental biochemical and cellular processes via interactions with a variety of partners to promote proliferation, invasion, survival, drug resistance, and metastasis in preclinical studies of cancer. Here, we review the diagnostic and therapeutic potential of HOTAIR as well as the molecular mechanisms underlying the dysregulation of its expression and function in cancer. We also discuss the challenges to capitalizing on HOTAIR for more effective patient care along with future directions founded on the recent exciting advances in our knowledge of HOTAIR in cancer.
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http://dx.doi.org/10.1016/j.canlet.2019.04.016DOI Listing
July 2019

Ether-compatible sulfurized polyacrylonitrile cathode with excellent performance enabled by fast kinetics via selenium doping.

Nat Commun 2019 03 4;10(1):1021. Epub 2019 Mar 4.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, 430074, Wuhan, China.

Sulfurized polyacrylonitrile is suggested to contain S (n ≤ 4) and shows good electrochemical performance in carbonate electrolytes for lithium sulfur batteries. However inferior results in ether electrolytes suggest that high solubility of LiS (n ≤ 4) trumps the limited redox conversion, leading to dissolution and shuttling. Here, we introduce a small amount of selenium in sulfurized polyacrylonitrile to accelerate the redox conversion, delivering excellent performance in both carbonate and ether electrolytes, including high reversible capacity (1300 mA h g at 0.2 A g), 84% active material utilization and high rate (capacity up to 900 mA h g at 10 A g). These cathodes can undergo 800 cycles with nearly 100% Coulombic efficiency and ultralow 0.029% capacity decay per cycle. Polysulfide dissolution is successfully suppressed by enhanced reaction kinetics. This work demonstrates an ether compatible sulfur cathode involving intermediate LiS (n ≤ 4), attractive rate and cycling performance, and a promising solution towards applicable lithium-sulfur batteries.
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http://dx.doi.org/10.1038/s41467-019-08818-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399341PMC
March 2019

Tuning the structure of bifunctional Pt/SmMnO interfaces for promoted low-temperature CO oxidation activity.

Nanoscale 2019 Apr;11(17):8150-8159

State Key Laboratory of Digital Manufacturing Equipment and Technology and School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, Hubei, People's Republic of China.

The interfacial structure of metal-oxide composite catalysts plays a vital role in heterogeneous catalysis, which is crucial to the adsorption and activation of reactants. Herein, the interfacial effects of bare and Fe/Co/Ni doped SmMn2O5 mullite oxide supported Pt clusters on CO oxidation have been investigated by first-principles based microkinetics analysis. A robust formation of Pt/Mn2 trimer structures is demonstrated at the bifunctional interfaces irrespective of the Ptn cluster's size, which can provide spatially separated sites for CO adsorption and O2 dissociation. The binding strength of CO at the interfacial Pt sites is in the optimal range due to the charge transfer from Pt clusters to oxide, while the strong polarization of Mn2 dimers induced by Pt clusters with stable three-dimensional morphologies can lower the energy barrier of O2 dissociation. Based on the microkinetics analysis, the O2 dissociation is the rate-determining step in the full CO oxidation cycle, and the introduction of Mn-Fe hetero-dimers at the interface is predicted to further enhance the low temperature CO oxidation activity of Pt/SmMn2O5 catalysts.
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http://dx.doi.org/10.1039/c8nr09054hDOI Listing
April 2019

Development of a scanning probe microscopy integrated atomic layer deposition system for successive monitoring of thin film growth.

Rev Sci Instrum 2018 Dec;89(12):123702

State Key Laboratory of Digital Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, Hubei 430074, People's Republic of China.

A dual chamber system integrated with atomic layer deposition (ALD) and atomic force microscopy (AFM) was developed for the successive monitoring of nanoparticles to thin film growth process. The samples were fabricated in the ALD chamber. A magnetic transmission rod enabled sample transferring between the ALD and the AFM test chambers without breaking the vacuum, avoiding possible surface morphology change when frequently varying the growth condition and oxidation under ambient condition. The sample transmission also avoids deposition and contamination on the AFM tip during the successive testing. The sample stage has machined a group of accurate location pinholes, ensuring the 10 m measurement consistency. As a demonstration, the platinum thin films with different thickness were fabricated by varying ALD cycles. The surface morphology was monitored successively during the deposition. Under vacuum with controlled oxygen partial pressure, the aging and sintering phenomenon of particles has been studied in the AFM testing chamber after high temperature treatment. The integrated AFM/ALD instrument is potentially a powerful system for monitoring the thin film preparation and characterization.
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http://dx.doi.org/10.1063/1.5042463DOI Listing
December 2018

The long noncoding RNA LINC00312 induces lung adenocarcinoma migration and vasculogenic mimicry through directly binding YBX1.

Mol Cancer 2018 11 23;17(1):167. Epub 2018 Nov 23.

Institute of Medical Sciences, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Xiangya Road 87th, Changsha, 410008, Hunan, People's Republic of China.

Vasculogenic mimicry (VM) gives rise to tumor neovascularization that is critical for tumor growth and metastasis. Long non-coding RNAs (lncRNAs) have been implicated in diverse and fundamental biological processes. LINC00312 is associated with lung adenocarcinoma. In this study, we found that LINC00312 induced migration, invasion and VM of lung cancer cells by direct binding to the transcription factor Y-Box Binding Protein 1 (YBX1). Moreover, we demonstrated that YBX1 is associated with different fragments within 0-2410 nt 5'region of LINC00312. In addition, LINC00312 is associated with VM in 124 lung adenocarcinoma clinical specimens. The results suggest that LINC00312 is a promising therapeutic and diagnostic target for lung adenocarcinoma.
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http://dx.doi.org/10.1186/s12943-018-0920-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260658PMC
November 2018

Pretreatment with cathelicidin-BF ameliorates Pseudomonas aeruginosa pneumonia in mice by enhancing NETosis and the autophagy of recruited neutrophils and macrophages.

Int Immunopharmacol 2018 Dec 26;65:382-391. Epub 2018 Oct 26.

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China. Electronic address:

Although the antimicrobial peptide cathelicidin-BF shows minimal cytotoxicity in mammalian cells and has excellent direct killing effects on multidrug-resistant clinical pathogens such as Pseudomonas aeruginosa, its clinical application is precluded by its high sensitivity to serum proteases. Here, we demonstrate that intravenous administration of cathelicidin-BF after P. aeruginosa infection did not increase the survival rate of mice with acute pneumonia but that pretreatment with cathelicidin-BF ameliorated pneumonia by effectively activating innate immunity. Enhanced neutrophil extracellular trap (NET) activation and release (NETosis) are key processes for capturing and killing bacteria, concomitantly enhanced macrophage clearance activity, including phagocytosis and autophagy, may eliminate NETs early enough to prevent severe tissue damage. Our study not only suggests a possible approach for applying cathelicidin-BF in vivo but also provides a possible defense strategy against multidrug-resistant pathogens, i.e., efficiently activation of innate immunity.
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http://dx.doi.org/10.1016/j.intimp.2018.10.030DOI Listing
December 2018