Publications by authors named "Bin Qiu"

339 Publications

Content-Noise Complementary Learning for Medical Image Denoising.

IEEE Trans Med Imaging 2021 Sep 16;PP. Epub 2021 Sep 16.

Medical imaging denoising faces great challenges, yet is in great demand. With its distinctive characteristics, medical imaging denoising in the image domain requires innovative deep learning strategies. In this study, we propose a simple yet effective strategy, the content-noise complementary learning (CNCL) strategy, in which two deep learning predictors are used to learn the respective content and noise of the image dataset complementarily. A medical image denoising pipeline based on the CNCL strategy is presented, and is implemented as a generative adversarial network, where various representative networks (including U-Net, DnCNN, and SRDenseNet) are investigated as the predictors. The performance of these implemented models has been validated on medical imaging datasets including CT, MR, and PET. The results show that this strategy outperforms state-of-the-art denoising algorithms in terms of visual quality and quantitative metrics, and the strategy demonstrates a robust generalization capability. These findings validate that this simple yet effective strategy demonstrates promising potential for medical image denoising tasks, which could exert a clinical impact in the future. Code is available at: https://github.com/gengmufeng/CNCL-denoising.
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http://dx.doi.org/10.1109/TMI.2021.3113365DOI Listing
September 2021

Developing the surgical technique reporting checklist and standards: a study protocol.

Gland Surg 2021 Aug;10(8):2591-2599

Editorial Office, AME Publishing Company, Hong Kong, China.

Background: Standardized and transparent reporting of surgical technique is the cornerstone of effective dissemination, implementation and improvement. However, current reporting of surgical techniques is inadequate. The existing guidelines potentially applied to guide surgical technique reporting are with a minimal highlight of the surgical technique, lack requirements explaining what extent and dimensions need to be described in detail, or are unlikely to extrapolate to a wide range of surgical techniques. This study aims to formulate a rigorous protocol to develop a surgical technique reporting checklist and standards (SUPER) that defines what a clear, comprehensive and detailed surgical technique report should be contained.

Methods: This protocol is designed following the classic guidance for developing reporting guidelines recommended by the EQUATOR network.

Results: The development team will consist of surgeons (~80%), methodologists, and journal editors. The draft checklist sources will include a scoping review of existing reporting guidelines related to surgical technique, surgical technique articles from 15 top journals published in the last year, and brainstorming by the multidisciplinary development team. The final SUPER checklist will be formed after three rounds of Delphi surveys, one round of face-to-face meeting, and a month-long pilot test. The SUPER checklist will be published as open-access and be used in combination with existing reporting guidelines related to surgical techniques (e.g., IDEAL). This protocol will steer the SUPER checklist's development, allowing us to further elaborate surgical technique reporting for all surgical specialties, and enabling a more favorable experience for surgeons, nurses, medical students, residents, editors, and reviewers.

Trial Registration: This trial is registered at the EQUATOR network on December 18th, 2020. Available at: https://www.equator-network.org/library/reporting-guidelines-under-development/reporting-guidelines-under-development-for-other-study-designs/.
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http://dx.doi.org/10.21037/gs-21-312DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8411094PMC
August 2021

Bunge: A Review on Botany, Phytochemistry, Pharmacology, and Applications.

Front Pharmacol 2021 30;12:708549. Epub 2021 Aug 30.

Department of Pharmacy, Baotou Medical College, Baotou, China.

Bunge (Sapindaceae) is a native Chinese plant with promising applications as a biofuel feedstock and a source of novel drugs. Historical records and documents from different periods have mentioned the use of and its botanical constituents in treating diseases, highlighting its central role in Chinese and Mongolian traditional medicinal therapies. Phytochemical research has focused on the husks, leaves, trunks, and branches of this herb. A total of 278 chemical compounds have been isolated and divided into 8 categories: triterpenoids, flavonoids, phenylpropanoids, steroids, phenols, fatty acids, alkaloids, and quinones. Modern pharmacological studies on have demonstrated positive effects on learning and memory, as well as anti-inflammatory, anti-tumor, and anti-oxidative properties. This review provides a comprehensive analysis of the available research on , focusing on the relationship between chemical constituents, traditional uses, and pharmacological effects. We also assess the potential for therapeutic and other applications of this plant in support of further research and development of .
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http://dx.doi.org/10.3389/fphar.2021.708549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435727PMC
August 2021

A simplified model for determining the cutting plane during thoracoscopic anatomical partial lobectomy of the right lower lobe.

Transl Lung Cancer Res 2021 Jul;10(7):3203-3212

Division of Thoracic Surgery, The Ohio State University Wexner Medical Center, Columbus, OH, USA.

Background: Few studies have examined the use of two-dimensional computed tomography (2D CT) and three-dimensional (3D) reconstruction images to determine the intersegmental plane (ISP) for pulmonary segmentectomy, but a systematic approach and nomenclature are currently lacking. This current study used 3D reconstruction of CT imaging to analyze variations in the right lower lobe's pulmonary ISP and created a simplified model to determine the optimum cutting plane (CP) for clinical application for operative planning and use during thoracoscopic anatomical partial lobectomy (APL).

Methods: Between January 2018 and October 2019, 325 patients with pulmonary lesions were identified who underwent thin-slice CT scans of the chest. The ISPs were identified by analyzing the 2D CT scans and 3D reconstruction images and the anatomical characteristics segmental boundary. The CP for the thoracoscopic procedure was then determined within the safe surgical margins, and a simplified CP model was created.

Results: The boundary between adjacent lung segment A and segment B was be expressed as "ISP: Sa-Sb". The ISP was divided into venous ISP (VISP), arterial ISP (AISP), and bronchial ISP (BISP). The proposed model of the CP can be expressed as follows: CP (f) = (V/A/B) ISP (x) + (V/A/B) sub ISP (y) + (V/A/B) sub-sub ISP (z).

Conclusions: This report is a first attempt to provide a nomenclature for identifying the ISP, and create a simplified model for determining the CP for thoracoscopic partial lobectomy.
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http://dx.doi.org/10.21037/tlcr-21-525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350089PMC
July 2021

Evidence on reporting guidelines for surgical technique in clinical disciplines: a scoping review protocol.

Gland Surg 2021 Jul;10(7):2325-2333

World Health Organization (WHO) Collaborating Centre for Guideline Implementation and Knowledge Translation, Lanzhou, China.

Background: The reporting of surgical techniques is of mixed quality, with most at a very minimal level. Reporting guidelines that could be applied to guide surgical technique reporting vary in methodology for development, discipline coverage, dimension coverage and detail requested. However, a scoping review that could indicate the gaps and efforts needed in surgical technique reporting guidelines is lacking and warranted. This study aims to design a methodological rigour protocol to guide the development of a scoping review of surgical technique reporting guidelines.

Methods: This protocol is designed following the 2020 manual proposed by the Joanna Briggs Institute. To further ensure the soundness of the protocol, we also included multidisciplinary professionals (including methodologists, clinicians, and journal editors) to refine the protocol.

Discussion: Seven key steps for developing the scoping review are identified and presented in detail, including (I) identifying the research questions; (II) inclusion criteria; (III) search strategy; (IV) source of evidence selection; (V) data extraction; (VI) analysis of the evidence; and (VII) presentation of the results. Guided by this protocol, the subsequent scoping review will inform us the overview of surgical technique reporting guidelines and precisely guide our direction and next steps in improving surgical technique reporting guidelines.

Trial Registration: This protocol is not registered as the PROSPERO database only accepts registration of systematic review protocols while does not accept registration of scoping review protocols.
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http://dx.doi.org/10.21037/gs-21-311DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340332PMC
July 2021

Adjuvant Radiotherapy for Keloids.

Aesthetic Plast Surg 2021 Aug 20. Epub 2021 Aug 20.

The Twelfth Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China.

Radiotherapy is one of the therapeutic methods for keloids, and the irradiation technique has innovated from superficial X-ray to brachytherapy after decades of clinical practice. At present, the application of adjuvant radiotherapy has been widely accepted by clinicians, while the consensus of optimal adjuvant radiotherapy strategies for keloids has not been reached. Factors such as radiation timing, dose, fractions, and lesion locations may be associated with the clinical outcomes of patients with keloids after radiotherapy while a comprehensive review is lacking. Herein, this review summarized the published literature of adjuvant radiotherapy for keloids involving mechanism, timing, dose, fractions, and complications, etc., which may facilitate clinical decision making.Level of Evidence III This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02442-wDOI Listing
August 2021

Comparative study of deep neural networks with unsupervised Noise2Noise strategy for noise reduction of optical coherence tomography images.

J Biophotonics 2021 Aug 12:e202100151. Epub 2021 Aug 12.

Institute of Medical Technology, Peking University Health Science Center, Peking University, Beijing, China.

As a powerful diagnostic tool, optical coherence tomography (OCT) has been widely used in various clinical setting. However, OCT images are susceptible to inherent speckle noise that may contaminate subtle structure information, due to low-coherence interferometric imaging procedure. Many supervised learning-based models have achieved impressive performance in reducing speckle noise of OCT images trained with a large number of noisy-clean paired OCT images, which are not commonly feasible in clinical practice. In this article, we conducted a comparative study to investigate the denoising performance of OCT images over different deep neural networks through an unsupervised Noise2Noise (N2N) strategy, which only trained with noisy OCT samples. Four representative network architectures including U-shaped model, multi-information stream model, straight-information stream model and GAN-based model were investigated on an OCT image dataset acquired from healthy human eyes. The results demonstrated all four unsupervised N2N models offered denoised OCT images with a performance comparable with that of supervised learning models, illustrating the effectiveness of unsupervised N2N models in denoising OCT images. Furthermore, U-shaped models and GAN-based models using UNet network as generator are two preferred and suitable architectures for reducing speckle noise of OCT images and preserving fine structure information of retinal layers under unsupervised N2N circumstances.
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http://dx.doi.org/10.1002/jbio.202100151DOI Listing
August 2021

Photoelectrochemical Biosensor for MicroRNA-21 Based on High Photocurrent of TiO/Two-Dimensional Coordination Polymer CuCl(MBA) Photoelectrode.

Anal Chem 2021 08 29;93(31):11010-11018. Epub 2021 Jul 29.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.

Conventional photosensitive materials such as TiO suffer from restricted absorption in the ultraviolet region, fast recombination of photogenerated electron-hole pairs, and a lack of functional groups for biocoupling, which hinder their application in photoelectrochemical (PEC) biosensing. Herein, a new coordination polymer (CP) based on Cu(I), chloridion, and 4-mercaptobenzoic acid (MBA) has been designed and synthesized (called CuCl(MBA)). The prepared p-type CuCl(MBA) exhibits visible-light absorption due to its narrow optical band gap (2.59 eV), and its proper band edge position enables it to form a p-n junction with TiO. Through layer-by-layer assembling, the photocurrent intensity of the CuCl(MBA)/TiO/FTO composite photoelectrode was 3.7-fold higher than that of a TiO/FTO electrode and 35-fold higher than a CuCl(MBA)/FTO electrode. The potential enhancement mechanism was discussed, which lies in the contributions of CuCl(MBA) in enhancing absorption in the visible-light region and boosting the separation of electron-hole pairs of TiO by the p-n junction. Furthermore, CuCl(MBA) nanosheets can realize bioconjugation directly, thanks to its abundant carboxyl groups. The CuCl(MBA)/TiO/FTO composite photoelectrodes were applied to develop a sensitive PEC biosensor for microRNA-21 (model target). By subtly exploiting the energy transfer between CuCl(MBA) and Au nanoparticles (AuNPs), AuNPs served as effective quenchers. In the presence of the target, AuNP-labeled sDNA1 connected to the electrode surface, and thus, a decreased photocurrent was obtained. The proposed biosensor has a low detection limit of 0.29 fM (S/N = 3), good selectivity, and reproducibility. The proposed system was applied to monitor microRNA in cancer cells with satisfying results.
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http://dx.doi.org/10.1021/acs.analchem.1c02267DOI Listing
August 2021

Stereotactic Ablative Brachytherapy: Recent Advances in Optimization of Radiobiological Cancer Therapy.

Cancers (Basel) 2021 Jul 12;13(14). Epub 2021 Jul 12.

Department of Radiation Oncology, Peking University Third Hospital, Beijing 100191, China.

Brachytherapy (BT), a type of focal anti-cancer radiotherapy, delivers a highly focused radiation dose to localized tumors, sparing surrounding normal tissues. Recent technological advances have helped to increase the accuracy of BT and, thus, improve BT-based cancer treatment. Stereotactic ablative brachytherapy (SABT) was designed to improve the ablative effect of radiation, which was achieved via improved image guidance, and calculation of ablative dose, shorter treatment duration, and better organ preservation. Recently collected data characterized SABT as having the potential to cure various early-stage cancers. The method provides higher tumor control rate levels that were previously achievable only by surgical resection. Notably, SABT is suitable for application with unresectable malignancies. However, the pathological assessment of SABT irradiated tumors is limited due to difficulties in specimen acquisition. Prostate, lung, liver, and gynecological cancers are the most commonly reported SABT-treated malignancies. This study will give an overview of SABT, focusing on the advances in SABT optimization, and provide insights on the future benefits of the combined application of SABT with cancer immunotherapies.
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http://dx.doi.org/10.3390/cancers13143493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304109PMC
July 2021

Homogeneous photoelectrochemical biosensor for microRNA based on target-responsive hydrogel coupled with exonuclease III and nicking endonuclease Nb.BbvCI assistant cascaded amplification strategy.

Mikrochim Acta 2021 07 22;188(8):267. Epub 2021 Jul 22.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian, 350116, People's Republic of China.

MicroRNAs can serve as biomarkers for many cancers, so it is significant to develop simple and sensitive strategies for microRNAs detection. Photoelectrochemical (PEC) detection has the advantages of simple equipment and high sensitivity. But in conventional PEC DNA sensors, tedious immobilization procedures of photoactive materials and capture probes on electrode surfaces are inevitable. To overcome those limitations, a homogeneous PEC biosensor based on target-responsive hydrogels has been developed (miRNA-155 has been chosen as a model target). PEC signal molecules (TiO nanoparticles, TiO NPs) were embedded in DNA hydrogels formed by hyaluronic acid sodium salt, amine-modified DNA double strands, and polyethylenimine rich in amine groups. In the presence of the target, DNA double strands in hydrogel were nicked by endonuclease and TiO NPs were released to the supernate and a high PEC response was obtained when collecting the supernate for PEC test, while almost no TiO NPs released in the absence of the target. Thanks to the exonuclease III and nicking endonuclease Nb.BbvCI-assisted cascaded amplification strategy, the proposed biosensor exhibits high sensitivity toward miRNA-155 with a low detection limit of 0.41 fM and a wide linear range from 1.0 fM to 100 pM. Since this method circumvents tedious electrode modification procedures, the proposed technique exhibits the advantages of simplicity and good reproducibility. Moreover, the prepared hydrogels have outstanding storage stability, so that they can be prepared in advance and shorten detection time. This biosensing platform provides a versatile strategy for the construction of homogeneous PEC biosensors for the detection of diverse targets. Photoelectrochemical detection techniques have been coupled with controlled release system to develop an immobilization-free microRNA biosensor. High sensitivity has been realized based on cascaded signal amplification strategy, and the proposed biosensor has been applied to detect the target in real sample with satisfied results. Since no tedious electrode modifications, the proposed homogeneous PEC sensor exhibits high reproducibility and good stability.
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http://dx.doi.org/10.1007/s00604-021-04935-6DOI Listing
July 2021

Long-Term Safety and Efficacy of CT-Guided I Radioactive Seed Implantation as a Salvage Therapy for Recurrent Head and Neck Squamous Carcinoma: A Multicenter Retrospective Study.

Front Oncol 2021 1;11:645077. Epub 2021 Jul 1.

Department of Radiation Oncology, Peking University 3rdHospital, Beijing, China.

Purpose: To investigate the safety and efficacy of CT-guided I radioactive seed implantation (RSI) as a salvage therapy for recurrent head and neck squamous carcinoma (rHNSC) after external beam radiotherapy (EBRT) or surgery.

Materials And Methods: This is a multicenter retrospective study of 113 patients (83 males; median age 57 years) with rHNSC who underwent CT-guided I RSI between February 2003 and December 2017. Of the included patients, 107 patients previously received EBRT and 65 patients received surgery and all were ineligible or rejected for salvage surgery and/or repeat EBRT.

Results: During a median follow-up duration of 20 months (range, 3-152 months), 87 patients died. The 1-, 2-, 3-, and 5-year local control rate were 57.4%, 41.8%, 29.3%, and 15.2%, respectively. The median time to progression was 15 months [95% confidence interval (CI), 6.1-23.9 months]. The median overall survival (OS) was 20 months (95% CI, 12.4-27.6 months). The 1-, 2-, 3-, and 5-year OS rate were 63.6%, 44.6%, 29.9%, and 21.7%, respectively. Univariate and multivariate analyses revealed that KPS score and postoperative D90 were significantly associated with patients' OS. The complications were mainly grade I/II skin and mucosal reactions: 18 cases (15.9%) of grade I/II and eight cases (7.0%) of grade III radiation dermatitis, and 14 cases (12.4%) of grade I/II and three cases (2.7%) grade III mucosal reactions. No grade IV or severer complications were found.

Conclusion: CT-guided I RSI may be safe as a salvage therapy for rHNSC after EBRT/surgery, yielding promising efficacy compared with historical data. KPS score and postoperative D90 may be significantly associated with OS.
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http://dx.doi.org/10.3389/fonc.2021.645077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281320PMC
July 2021

Highly Sensitive Homogeneous Electrochemiluminescence Biosensor for Alkaline Phosphatase Detection Based on Click Chemistry-Triggered Branched Hybridization Chain Reaction.

Anal Chem 2021 07 16;93(29):10351-10357. Epub 2021 Jul 16.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350116, China.

Alkaline phosphatase (ALP) has been used as a diagnostic index of clinical diseases since its expression level is closely related to many pathological processes. In this work, a highly sensitive electrochemiluminescence (ECL) method for the determination of ALP based on a click chemistry-induced branched hybridization chain reaction (BHCR) for signal amplification and ultrafiltration technology for the separation of homogeneous amplification products is introduced. ALP can release copper ions from a Cu/PPi complex by hydrolyzing pyrophosphoric acid, which initiates click chemistry in the system. A BHCR amplification is triggered afterward by the long single-stranded DNA (ssDNA) generated by click chemistry, resulting in a three-dimensional double-stranded DNA (dsDNA) with a large molecular weight. Based on the characteristic that Ru(phen) can stably insert into the groove of dsDNA, a large amount of Ru(phen) is retained together with the amplified product after ultrafiltration, and therefore a significantly enhanced ECL signal can be obtained. The test results show that this method can be used for the quantitative determination of ALP ranging from 0.002 to 50 U/L, with a detection limit of 0.7 mU/L. This method has also been confirmed to have good selectivity and anti-interference, and the results of the analysis of the ALP content in the diluted serum samples are satisfactory, showing great application potential in clinical diagnosis.
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http://dx.doi.org/10.1021/acs.analchem.1c02094DOI Listing
July 2021

Brønsted Acid-Catalyzed Minisci-Type Cross-Dehydrogenative Coupling of -Heteroaromatics and Cyclic Ethers.

J Org Chem 2021 Jul 28;86(14):9299-9305. Epub 2021 Jun 28.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China.

Brønsted acid-catalyzed direct C(sp)-H alkylation of -heteroaromatics with cyclic ethers via a cross-dehydrogenative coupling reaction in the presence of benzoyl peroxide was developed. This methodology successfully provided an easy access to a variety of alkyl-substituted quinoxaline, benzoimidazole, pyrazine, pyrimidine, quinoline, isoquinoline, and pyridine derivatives in up to 94% yield under metal-free conditions.
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http://dx.doi.org/10.1021/acs.joc.1c00337DOI Listing
July 2021

The accuracy and dosimetric analysis of 3D-printing non-coplanar template-assisted iodine-125 seed implantation for recurrent chest wall cancer.

J Contemp Brachytherapy 2021 Jun 18;13(3):273-279. Epub 2021 May 18.

Department of Radiation Oncology, Peking University Third Hospital, Beijing, China.

Purpose: To investigate the accuracy and dosimetry of 3D-printing non-coplanar template (3D-PNCT)-assisted computed tomography (CT)-guided iodine-125 seed implantation (I-SI) for recurrent chest wall cancer (rCWC).

Material And Methods: This was a retrospective study of 19 patients with 22 rCWC treated with 3D-PNCT-assisted CT-guided I-SI, from Mar 2017 to Mar 2020 in our institute.

Results: Mean needle entrance deviation was 4.50 ±2.70 mm, mean angular deviation was 3.40 ±3.10 degrees, and mean depth deviation was 5.20 ±5.20 mm. No significant difference was found for dosimetric parameters (except conformity index) between pre-plan and post-plan; D, D, V, V, and V were 157.74 ±24.23 and 151.71 ±33.62 ( = 0.228), 85.36 ±34.09 and 70.46 ±23.48 ( = 0.067), 0.93 ±0.04 and 0.90 ±0.07 ( = 0.068), 0.64 ±0.16 and 0.64 ±0.16 ( = 0.984), and 0.35 ±0.17 and 0.37 ±0.18 ( = 0.382) for pre-plan and post-plan, respectively. Conformity index, external index, and homogeneity index were 0.57 ±0.16 and 0.52 ±0.15 ( = 0.007), 0.73 ±0.55 and 0.79 ±0.53 ( = 0.096), and 0.31 ±0.15 and 0.30 ±0.14 ( = 0.504) for pre-plan and post-plan, respectively. Median follow-up time was 8 months (range, 3-30 months). Complete response was observed in 4/22 (18.1%), partial response in 13/22 (59.1%), stable disease in 4/22 (18.1%), and progression disease in 1/22 (4.5%) of the cancers. Among patients with pain before I-SI, pain relief rate was 87.5% (7/8). No peri-operative complications of more than grade 2 were observed.

Conclusions: 3D-PNCT-assisted CT-guided I-SI may be safe and feasible as palliative therapy for non-surgical candidates and painful patients with rCWC.
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http://dx.doi.org/10.5114/jcb.2021.106250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170518PMC
June 2021

Electrochemiluminescence biosensor for thrombin detection based on metal organic framework with electrochemiluminescence indicator embedded in the framework.

Biosens Bioelectron 2021 Oct 29;189:113374. Epub 2021 May 29.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China. Electronic address:

Ru(dcbpy)-polyethyleneimine-L-lysine (Ru-PEI-L-lys) had been immobilized on metal organic frameworks (ZIF-8) to form an electrochemiluminescent(ECL) indicator (Ru-PEI-L-lys-ZIF-8). In this ECL indicator, PEI-L-lys is used as a co-reactant. Platinum nanoparticles (PtNPs) has been mixed with Ru-PEI-L-lys-ZIF-8 to form a thin film to increase the electron transfer rate and enhanced the ECL response of the system. The prepared material had been characterized carefully and been combined with high selectivity of aptamer to develop a ECL biosensor for thrombin detection. RecJ exonuclease (an ssDNA specific exonuclease) assistant target recycling amplification has been adopted to enhance the sensitivity of the system. The ECL response of the system has a linear relationship with logarithm of thrombin concentration in the range of 1 fM to 10 pM with a detection limit of 0.02 aM. This work not only provides a new strategy for the design and synthesis of high performance and stable ECL indicator, but also opens up a new approach for the development of highly sensitive ECL sensors for biological analysis.
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http://dx.doi.org/10.1016/j.bios.2021.113374DOI Listing
October 2021

Dosimetric comparison of computed tomography-guided iodine-125 seed implantation assisted with and without three-dimensional printing non-coplanar template in locally recurrent rectal cancer: a propensity score matching study.

J Contemp Brachytherapy 2021 Feb 18;13(1):18-23. Epub 2021 Feb 18.

Department of Radiation Oncology, Peking University Third Hospital, Beijing, People's Republic of China.

Purpose: To compare post-implant dosimetric parameters of computed tomography (CT)-guided radioactive iodine-125 (I) seed (RIS) implantation assisted with and without three-dimensional printing non-coplanar template (3D-PNCT) in locally recurrent rectal cancer (LRRC).

Material And Methods: One hundred and fifty-five LRRC patients treated by CT-guided RIS implantation assisted with or without 3D-PNCT from October 2003 to May 2019 were included in this study. Propensity score matching (PSM) method (1 : 1) was used to adjust for differences between the 3D-group (with 3D-PNCT) and the CT-group (without 3D-PNCT). After PSM, dosimetric parameters [D (dose that covered 90% of target volume), D (dose that covered 100% of target volume), V (percentage of gross tumor volume (GTV) receiving 100% of prescription dose), V (percentage of GTV receiving 150% of prescription dose), HI (homogeneity index), CI (conformity index), and EI (external index)] of the two groups were compared.

Results: After PSM, 45 pairs of matched cases were selected for analysis and differences in variables between the two groups were balanced. For the 3D-group, median values of D, D, V, V, EI, and HI were 142.6 Gy (73.7-218.2 Gy), 73.7 Gy (26.2-169.3 Gy), 94.1% (74.3-100%), 71.8% (35.4-98.3%), 0.7 (0.1-30.7), and 0.20 (0-0.60), respectively, and corresponding values were 119.9 Gy (39.8-159.3 Gy), 47.0 Gy (13.0-200.9 Gy), 89.9% (38.6-100%), 62.8% (14.8-100%), 0.39 (0-11.01), and 0.30 (0-0.95), respectively, for the CT-group. Parameters including D, D, V, V, and EI in the 3D-group were significantly higher than those in the CT-group ( < 0.001, < 0.001, < 0.001, < 0.001, and = 0.006, respectively).

Conclusions: 3D-PNCT can improve the accuracy of radioactive seed implantation by increasing the dose delivered to the tumor and reducing the number of "cold" spots of dose.
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http://dx.doi.org/10.5114/jcb.2021.103582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117706PMC
February 2021

The surgical management of early-stage lung adenocarcinoma: is wedge resection effective?

J Thorac Dis 2021 Apr;13(4):2137-2147

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The aim of the study was to explore the outcomes of wedge resection on patients with early-stage lung adenocarcinoma (LUAD) and further identify potential prognostic factors for these patients.

Methods: A retrospective cohort of 190 patients (99 solitary LUAD and 91 multifocal LUAD) undergone wedge resection from October 2014 to September 2015 was established. Cox proportional-hazards model was used to evaluate the significant clinical prognostic factors. Further, data on patients with multifocal adenocarcinoma after segmentectomy were retrieved and propensity score matching was used to compare the outcomes of patients with multiple pulmonary nodules (MPNs) after wedge resection and segmentectomy.

Results: The 5-year overall survival (OS), progression-free survival (PFS), and lung cancer specific survival of the 190 patients after wedge resection were 95.5%, 87.9%, and 97.7%, respectively. Multivariable analysis showed that MPN [hazard ratio (HR) 3.07; 95% confidence interval (CI), 1.05-8.98] and solid-dominant lesions (HR 15.87; 95% CI, 2.38-105.84) were independently associated with worse PFS. Further, propensity score matching analysis showed that MPN patients had better PFS after segmentectomy compared with wedge resection (94% 80.9%, P=0.008). MPN patients were more likely to perform systematic mediastinal nodal sampling (95.6% 59.3%, P<0.001) after segmentectomy compared to patients who underwent wedge resection.

Conclusions: Wedge resection is a practical option for appropriately selected early-stage LUAD where tumor size is less than 2 cm and has a consolidation-to-tumor ratio ≤0.5. However, for MPNs, wedge resection may be not reliable and alternative procedures such as segmentectomy should be used.
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http://dx.doi.org/10.21037/jtd-20-3005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107550PMC
April 2021

Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.

Analyst 2021 May;146(10):3299-3304

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China.

The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection. In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system. The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily. The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained. But after the addition of HAase, which can hydrolyze HA in the hydrogel, the permeability of the membrane increased. Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly. The composite of the membrane, and the reaction conditions after the addition of HAase were optimized. Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
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http://dx.doi.org/10.1039/d1an00257kDOI Listing
May 2021

Highly Sensitive and Selective Photoelectrochemical Aptasensors for Cancer Biomarkers Based on MoS/Au/GaN Photoelectrodes.

Anal Chem 2021 05 7;93(19):7341-7347. Epub 2021 May 7.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350116, China.

An Au/GaN photoelectrode was prepared by sputtering 30 nm thick Au film on the surface of n-type gallium nitride (GaN). When the electrode contacts with multilayered molybdenum disulfide (MoS), photogenerated electrons and photogenerated holes transfer to MoS because of the band gap matching of MoS and GaN. The presence of Au promotes charge transfer and results in a greater recombination of electrons and holes; by this means, a more significant suppression of photocurrent can be detected. This characteristic has been coupled with the high selectivity of an aptamer and applied to develop a novel photoelectrochemical aptasensor for cancer biomarkers (alpha-fetoprotein (AFP) as a model). The aptamer of AFP was modified on the surface of the Au/GaN photoelectrode by Au-S bonds, which can bind to the target protein with high selectivity. Then, the transfer process of the charge carriers of GaN to MoS can be blocked by the target protein so that the suppression of photocurrent is reduced. The difference of the photocurrent in the presence and absence of AFP () showed a linear relationship with AFP concentration that ranged from 1.0-150 ng/mL ( = 0.9995), and the detection limit was 0.3 ng/mL. The standard addition recovery rates ranged from 85.2 to 91.7%. The method possessed good sensitivity and high selectivity for AFP detection. The developed biosensor can be modified to detect other cancer biomarkers by simply replacing the aptamer used.
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http://dx.doi.org/10.1021/acs.analchem.1c01197DOI Listing
May 2021

The effectiveness of three-dimensional reconstruction in the localization of multiple nodules in lung specimens: a prospective cohort study.

Transl Lung Cancer Res 2021 Mar;10(3):1474-1483

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The detection rate of multiple pulmonary nodules in computed tomography (CT) screening has increased significantly in recent years. In cases with multiple nodules within the same lung lobe or segment, it is often difficult for thoracic surgeons and pathologists to accurately locate all lesions in the surgically resected specimens. Therefore, the objective of our study was to use three-dimensional (3D) reconstruction in conjunction with 3D printing as an auxiliary method for localizing multiple small nodules in specimens after surgery and to evaluate its effectiveness.

Methods: A single-center prospective cohort study was conducted between September 2019 and September 2020 at the National Cancer Center (Beijing, China). In total, 43 surgical candidates with multiple nodules were recruited to undergo lobectomy or segmentectomy and 40 patients were ultimately enrolled in this study. With the assistance of 3D reconstruction/printing models, the obtained specimens were marked and then identified by a pathologist. The primary outcome was the success rate of nodule localization in the resected specimens, and the secondary outcome was the agreement rate between the pathological results of the samples and CT images.

Results: In the 40 patients enrolled, 126 nodules were detected by preoperative imaging, of which 124 nodules (positive rate: 98.4%) were successfully located in the resected specimens using 3D reconstruction/printing. For the 124 nodules, the agreement rate of the pathological results of samples and CT images with the assistance of 3D reconstruction/printing models was 100.0%.

Conclusions: The results show that 3D reconstruction/printing models allow for the rapid and accurate localization of nodules in resected specimens. Also, the pathological results of lesions show good agreement with the results of preoperative CT imaging, which is of great significance for further study into the clinicopathological characteristics and radiomics of multiple pulmonary nodules.
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http://dx.doi.org/10.21037/tlcr-21-202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044493PMC
March 2021

The Accuracy of Individualized 3D-Printing Template-Assisted I Radioactive Seed Implantation for Recurrent/Metastatic Head and Neck Cancer.

Front Oncol 2021 31;11:664996. Epub 2021 Mar 31.

Department of Radiation Oncology, Peking University Third Hospital, Beijing, China.

Purpose: To evaluate the accuracy of individualized 3D-printing template-assisted I radioactive seed implantation (3D-PT assisted I RSI) for recurrent/metastatic head and neck cancer.

Materials And Methods: From February 2017 to January 2020, clinical data of 41 patients (mean age, 58.5 ± 16.1 years; 28 males) with recurrent (48.8%)/metastatic (51.2%) head and neck cancer underwent individualized 3D-PT assisted I RSI under CT guidance in a single institute were retrospectively reviewed. Total 430 seed needles [mean, 10.5 (range 3-17) per patient] were inserted.

Results: All seed needles were inserted manually in a single attempt with the technical success rate of 100% without major perioperative complications. The mean needle's entrance deviation was 0.090 cm (95% Confidence Interval, 0.081-0.098). The mean intraoperative depth and angle of the needle were consistent with that of planned (6.23 ± 0.24 vs. 6.21 ± 0.24 cm, p = 0.903; 83.14 ± 3.64 vs. 83.09 ± 3.66 degrees, p = 0.985, respectively). The mean deviation between the needle's planned and intraoperative depth and angle was 0.168 ± 0.024 cm and 1.56 ± 0.14 degrees, respectively. The postoperative dosimetry parameters, including D90, D100, V100, V150, V200, conformity index, external index, and homogeneity index, were all well-coordinated with planned dosimetry without significant difference (p = 0.515, 0.662, 0.958, 0.865, 0.872, 0.278, 0.456, and 0.989, respectively).

Conclusions: Within the limitation of this study, individualized 3D-PT assisted I RSI may be accurate in obtaining favorable postoperative dosimetry for patients with recurrent/metastatic head and neck cancer.

Clinical Trial Registration: [website], identifier [registration number].
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http://dx.doi.org/10.3389/fonc.2021.664996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045752PMC
March 2021

Metabolomic analysis of antimicrobial mechanism of polysaccharides from Sparassis crispa based on HPLC-Q-TOF/MS.

Carbohydr Res 2021 May 2;503:108299. Epub 2021 Apr 2.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel Farming and Processing, Fuzhou University, Fuzhou, Fujian, 350108, PR China. Electronic address:

Abuse of antibiotics makes antibiotic-resistance become a huge challenge in bacterial infection treatment. The discovery of new antibiotics is of great significance to human health. In this study, the antibacterial mechanism of Sparassis crispa polysaccharides (SCPs) was explored. The SCPs isolated from Sparassis crispa was composed of fucose, glucose and galactose with a molar ratio of 0.043 : 0.652: 0.305. Bacteriostatic tests showed SCPs inhibited the growth of Staphylococcus aureus better than Escherichia coli's, and damage to bacteria was observed under scanning electron microscopy. Metabolomic analysis based on HPLC-Q-TOF/MS indicated that SCPs disrupted metabolism of the glycolysis and tricarboxylic acid cycle pathways in S. aureus. The variations of fructose-1,6-diphosphate, 1,3-diphosphoglycerol, succinate and oxaloacetate were significant, whose systematic changes accompanied with decrease of ATP in cells indicated that SCPs could exert antibacterial effects by inducing dysfunction of catabolism and energy metabolism. Our research confirmed the antibacterial properties of SCPs and provided a perspective for understanding antibacterial mechanism of polysaccharides from natural products through metabolomics technology.
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http://dx.doi.org/10.1016/j.carres.2021.108299DOI Listing
May 2021

Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes.

Biochem Biophys Res Commun 2021 05 17;552:84-90. Epub 2021 Mar 17.

Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China. Electronic address:

Background: Angiotensin II (Ang II), an important component of the renin-angiotensin system (RAS), plays a critical role in the pathogenesis of cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as a promising platform for studying personalized medicine for heart diseases. However, whether Ang II can induce the apoptosis of hiPSC-CMs is not known.

Methods: In this study, we treated hiPSC-CMs with different concentrations of Ang II [0 nM (vehicle as a control), 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, 100 μM, and 1 mM] for various time periods (24 h, 48 h, 6 days, and 10 days) and analyzed the viability and apoptosis of hiPSC-CMs.

Results: We found that treatment with 1 mM Ang II for 10 days reduced the viability of hiPSC-CMs by 41% (p = 2.073E-08) and increased apoptosis by 2.74-fold, compared to the control group (p = 6.248E-12). MYOG, which encodes the muscle-specific transcription factor myogenin, was also identified as an apoptosis-suppressor gene in Ang II-treated hiPSC-CMs. Ectopic MYOG expression decreased the apoptosis and increased the viability of Ang II-treated hiPSC-CMs. Further analysis of the RNA sequencing (RNA-seq) data illustrated that myogenin ameliorated Ang II-induced apoptosis of hiPSC-CMs by downregulating the expression of proinflammatory genes.

Conclusion: Our findings suggest that Ang II induces the apoptosis of hiPSC-CMs and that myogenin attenuates Ang II-induced apoptosis.
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http://dx.doi.org/10.1016/j.bbrc.2021.03.031DOI Listing
May 2021

Therapeutic and Prebiotic Effects of Five Different Native Starches on Dextran Sulfate Sodium-Induced Mice Model of Colonic Colitis.

Mol Nutr Food Res 2021 04 9;65(8):e2000922. Epub 2021 Mar 9.

Shandong Academy of Agricultural Sciences, Institute of Agro-Food Science and Technology, Jinan, 250100, China.

Scope: The availability of studies related to the effects of natural macronutrients on inflammatory bowel disease (IBD) remain relatively limited. This study investigates whether and to what extent the consumption of five different native starches alleviate the clinical symptoms and dysbiosis of gut microbiota associated with colitis.

Methods And Results: Using dextran sodium sulfate (DSS)-induced mouse model of colitis, the potential effects of native potato starch (PS), pea starch (PEAS), corn starch (CS), Chinese yam starch (CYS), and red sorghum starch (RSS) on the clinical manifestations and dysbiosis of gut microbiota are studied. Compared to CS and RSS, the consumption of PEAS, PS, and CYS significantly diminishes clinical enteritis symptoms, including reduced disease activity index, and the alleviated degree of colonic histological damage. Furthermore, the analysis of gut microbiota reveals the significant prebiotic characteristics of PEAS, PS and CYS, as indicated by the maintenance of gut microbiota hemostasis and the inhibition of typically pathogenic bacteria, including Escherichia coli and Helicobacter hepaticus.

Conclusion: Starches from potato, pea, and Chinese yam alleviate colitis symptoms in a mouse model, and also show significant prebiotic characteristics. These findings suggest a cost-effective and convenient dietary strategy for the management of IBD.
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http://dx.doi.org/10.1002/mnfr.202000922DOI Listing
April 2021

Convenient detection of HS based on the photothermal effect of [email protected] nanocubes using a handheld thermometer as readout.

Anal Chim Acta 2021 Mar 12;1149:338211. Epub 2021 Jan 12.

MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China.

Hydrogen sulfide (HS), as a hazardous gas, is often found around dump areas. Long term exposure can cause harm to health, it is highly necessary to develop some simple and sensitive methods for on-site HS detection. Herein, a convenient photothermal assay has been designed for the quantitation of HS using a handheld thermometer as readout. [email protected] nanocubes ([email protected] NCs), a core-shell nanocomposite with strong light absorption at ∼450 nm, was chosen as a novel photothermal agent in this study. Under the laser irradiation at 450 nm, the [email protected] NCs show a strong photothermal effect, and a significant temperature enhancement can be measured by the thermometer easily. The presence of HS can lead to the deposition of sulfur onto [email protected] NCs, altering the localized surface plasmon resonance absorption, size, surface composition, and morphology of [email protected] NCs and hence leading to the reduction of photothermal effect. The change of the temperature has a linear relationship with the HS concentration in the range of 0.5-80.0 μM with a detection limit of 0.35 μM. By combining with simple sample purification procedures, the developed method has been applied to detect HS in garbage odor gas with satisfactory results.
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http://dx.doi.org/10.1016/j.aca.2021.338211DOI Listing
March 2021

Treatment patterns, clinical outcomes, and healthcare resource use associated with advanced/metastatic lung cancer in China: protocol for a retrospective observational study.

Transl Lung Cancer Res 2020 Dec;9(6):2460-2468

Cancer Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Background: Lung cancer (LC) is the most common cancer worldwide. The prevalence of LC and rate of associated mortality are high and increasing faster in China than in Western countries. Non-small cell lung cancer (NSCLC) accounts for most LCs. This study aims to be the first large, multi-center, non-interventional retrospective study of treatment patterns (type/duration, number of lines, completion rate), real-world outcomes, and medical costs among Chinese patients with advanced/metastatic NSCLC (IIIb/IV) or extensive-stage small cell LC (ES-SCLC).

Methods: This study will enroll 8,800 patients (≥18 years, with a diagnosis of advanced/metastatic NSCLC made between 1 December 2013 to 30 November 2014) from 35 to 50 Chinese sites. Hospital information systems (HIS) and electronic medical records will be retrospectively reviewed, in adherence with regulatory and ethical requirements. Early-stage treatment (starting from 1 December 2010) of patients with recurrent disease or early disease progression will be examined. Data will be collected at baseline (diagnosis) and 6 and 12 months after this. Observation will end after 3 years or death. Data will be stratified by histology, staging, age, region, health insurance, and epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK) mutation status. Treatment duration and overall survival will be estimated using Kaplan-Meier curves. Descriptive statistics will be used for disease characteristics and patient demographics. Cox-proportional hazards models will be used to examine the impact of demographics/treatment on survival. Treatment patterns and outcome predictors will be explored using multivariate logistic regression.

Discussion: This protocol describes the methodology for collecting real-world data to guide evidence-based clinical practice and inform unmet needs in NSCLC treatment, with potential to identify gaps between guidelines and current practice.

Trial Registration: NCT03505515; data registered on ClinicalTrials.gov: 12h Apr., 2018.
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http://dx.doi.org/10.21037/tlcr-20-1269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815359PMC
December 2020

Study on the biosensor based on biomimetic PDA vesicles fluorescence resonance energy transfer for the determination of ovarian cancer marker miRNA-21.

Anal Sci 2021 Jan 8. Epub 2021 Jan 8.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University.

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http://dx.doi.org/10.2116/analsci.20P404DOI Listing
January 2021

Highly Reproducible and Sensitive Electrochemiluminescence Biosensors for HPV Detection Based on Bovine Serum Albumin Carrier Platforms and Hyperbranched Rolling Circle Amplification.

ACS Appl Mater Interfaces 2021 Jan 31;13(1):298-305. Epub 2020 Dec 31.

Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People's Republic of China.

Most DNA-based electrochemiluminescence (ECL) biosensors are established through the self-assembly of thiolated single-stranded DNA (ssDNA) probes on the Au electrode surface. Because of this random assembly process, a significant discrepancy exists in the distribution of a modified DNA film on different electrodes, which greatly affects the reproducibility of a biosensor. In this study, a porous bovine serum albumin (BSA) layer was first modified on the electrode surface, which can improve the position distribution and spatial orientation of the self-assembly ssDNA probe. It was then coupled with hyperbranched rolling circle amplification to develop the high-reproducibility-and-sensitivity ECL biosensor for human papillomavirus 16 E6 and E7 oncogene detection. In the presence of the target DNA, the surface of the electrode accumulates abundant amplified products through reaction, which contain double-stranded DNA (dsDNA) fragments of different lengths, followed by plentiful dichlorotris (1,10-phenanthroline) ruthenium(II) hydrate (Ru(phen), acting as an ECL indicator) insertion into grooves of dsDNA fragments, and a strong signal can be detected. There is a linear relationship between the signal and the target concentration range from 10 fM to 15 pM, and the detection limit is 7.6 fM (S/N = 3). After the BSA modification step, the relative standard deviation was reduced from 9.20 to 3.96%, thereby achieving good reproducibility. The proposed ECL strategy provides a new method for constructing high-reproducibility-and-sensitivity ECL biosensors.
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http://dx.doi.org/10.1021/acsami.0c20742DOI Listing
January 2021

A Numerical Model of a Perforated Microcantilever Covered with Cardiomyocytes to Improve the Performance of the Microcantilever Sensor.

Materials (Basel) 2020 Dec 28;14(1). Epub 2020 Dec 28.

Smart Materials and Advanced Structure Laboratory, School of Mechanical Engineering and Mechanics, Ningbo University, Ningbo 315211, China.

A few simple polymeric microsystems, such as microcantilever sensors, have recently been developed for the preliminary screening of cardiac toxicity. The microcantilever deflection produced by a change in the cardiomyocyte (CM) contraction force is important for understanding the mechanism of heart failure. In this study, a new numerical model is proposed to analyze the contractile behavior of CMs cultured on a perforated microcantilever surface for improving the performance of the microcantilever sensor. First, the surface traction model is used to investigate the bending displacement of the plain microcantilever. In order to improve the bending effect, a new numerical model is developed to analyze the bending behavior of the perforated microcantilever covered with CMs. Compared with the designed molds, the latter yields better results. Finally, a simulation analysis is proposed based on a finite element method to verify the presence of a preformed mold. Moreover, the effects of various factors on the bending displacement, including microcantilever size, Young's modulus, and porosity factor, are investigated. Both the simulation and numerical results have good consistency, and the maximum error between the numerical and simulation results is not more than 3.4%, even though the porosity factor reaches 0.147. The results show that the developed mold opens new avenues for CM microcantilever sensors to detect cardiac toxicity.
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http://dx.doi.org/10.3390/ma14010095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7795391PMC
December 2020

Brachytherapy for lung cancer.

Brachytherapy 2021 Mar-Apr;20(2):454-466. Epub 2020 Dec 24.

Department of Radiation Oncology, Peking University Third Hospital, Beijing, China. Electronic address:

Brachytherapy (BT) is a minimally invasive anticancer radiotherapeutic modality where the tumor is directly irradiated via a radioactive source that is precisely implanted in or adjacent to the tumor. BT for lung cancer may be conducted in the form of endobronchial BT and radioactive seed implantation (RSI-BT), mainly for nonsmall cell lung cancer (NSCLC). For patients with early-stage lung cancer who are not suitable for surgery or external beam radiotherapy (EBRT), BT may be used as an alternative treatment, and curative results could be achieved in certain patients with cancer confined to the trachea lumen. For patients with locally advanced/metastatic lung cancer, BT could be selectively applied alone or as a boost to EBRT, which could improve the local tumor control and patient's survival. In addition, BT is also useful as a salvage treatment in select patients with locally recurrent/residual lung cancer that failed other treatments (e.g., surgery, chemotherapy, and EBRT). However, clinical outcomes are mainly obtained from retrospective studies. Prospective studies are limited and needed. In recent years, the introduction of modern image guidance, novel radioactive seeds, BT treatment planning systems (BT-TPS), after-loading technique, and three-dimensional printing template (3D-PT) assistance, among others, have potentially improved the clinical outcomes of BT. However, a comprehensive review of BT with newly published literature was lacking. This review is to discuss BT for NSCLC based on recent literature published in PubMed.
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http://dx.doi.org/10.1016/j.brachy.2020.11.009DOI Listing
August 2021
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