Publications by authors named "Bin Ma"

548 Publications

High-quality-factor dual-band Fano resonances induced by dual bound states in the continuum using a planar nanohole slab.

Nanoscale Res Lett 2021 Sep 28;16(1):150. Epub 2021 Sep 28.

Key Laboratory of Advanced Micro-Structured Materials MOE, Institute of Precision Optical Engineering, School of Physics Science and Engineering, Tongji University, Shanghai, 200092, China.

In photonics, it is essential to achieve high-quality (Q)-factor resonances to improve optical devices' performances. Herein, we demonstrate that high-Q-factor dual-band Fano resonances can be achieved by using a planar nanohole slab (PNS) based on the excitation of dual bound states in the continuum (BICs). By shrinking or expanding the tetramerized holes of the superlattice of the PNS, two symmetry-protected BICs can be induced to dual-band Fano resonances and their locations as well as their Q-factors can be flexibly tuned. Physical mechanisms for the dual-band Fano resonances can be interpreted as the resonant couplings between the electric toroidal dipoles or the magnetic toroidal dipoles based on the far-field multiple decompositions and the near-field distributions of the superlattice. The dual-band Fano resonances of the PNS possess polarization-independent feature, and they can be survived even when the geometric parameters of the PNS are significantly altered, making them more suitable for potential applications.
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http://dx.doi.org/10.1186/s11671-021-03607-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479049PMC
September 2021

Therapeutic Effects of Stem Cells From Different Source on Renal Ischemia- Reperfusion Injury: A Systematic Review and Network Meta-analysis of Animal Studies.

Front Pharmacol 2021 2;12:713059. Epub 2021 Sep 2.

Evidence Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.

Although stem cell therapy for renal ischemia-reperfusion injury (RIRI) has made immense progress in animal studies, conflicting results have been reported by the investigators. Therefore, we aimed to systematically evaluate the effects of different stem cells on renal function of animals with ischemia-reperfusion injury and to compare the efficacies of stem cells from various sources. PubMed, Web of Science, Embase, Cochrane, CNKI, VIP, CBM, and WanFang Data were searched for records until April 2021. Two researchers independently conducted literature screening, data extraction, and literature quality evaluation. Seventy-two animal studies were included for data analysis. Different stem cells significantly reduced serum creatinine and blood urea nitrogen levels in the early and middle stages (1 and 7 days) compared to the negative control group, however there was no significant difference in the late stage among all groups (14 days); In the early stage (1 day), the renal histopathological score in the stem cell group was significantly lower than that in the negative control group, and there was no significant difference among these stem cells. In addition, there was no significant difference between stem cell and negative control in proliferation of resident cells, however, significantly less apoptosis of resident cells than negative control. In conclusion, the results showed that stem cells from diverse sources could improve the renal function of RIRI animals. ADMSCs and MDMSCs were the most-researched stem cells, and they possibly hold the highest therapeutic potential. However, the quality of evidence included in this study is low, and there are many risks of bias. The exact efficacy of the stem cells and the requirement for further clinical studies remain unclear.
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http://dx.doi.org/10.3389/fphar.2021.713059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444551PMC
September 2021

Azepine- or Azocine-Embedded Hexabenzocoronene Derivatives as Nitrogen-Doped Saddle or Saddle-Helix Nanographenes.

Angew Chem Int Ed Engl 2021 Sep 15. Epub 2021 Sep 15.

School of Chemical Science and Technology, Yunnan University, Kunmimg, 650500, P. R. China.

Two novel nitrogen-doped, hexa-peri-hexabenzocoronene (HBC)-based nanographenes (NGs) 1 and 2 bearing an azepine and an azocine at the fjord region, respectively, were synthesized and characterized. Notably, structure 1 was synthesized by Diels-Alder reaction of cyclic alkene and tetrachlorothiophene-S,S-dioxide, followed by Suzuki-Miyaura cross-coupling and Scholl-type reactions, which represents a modified strategy to construct NGs. The azo-heptagon-embedded NG 1 leads to a saddle shape, and the azo-octagon-embedded NG 2 exhibits a distorted saddle-helix conformation with the largest torsion angle recorded so far in [5]helicenes. As a result, the different structural topographies for NGs 1 and 2 lead to significant changes in the optical properties including UV absorption and fluorescent emission. Additionally, the 8π-heterocycles azepine and azocine in the NGs 1 and 2 exhibited obvious antiaromatic properties.
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http://dx.doi.org/10.1002/anie.202110538DOI Listing
September 2021

Caudad Insertion of Pedicle Screws Facilitates Interbody Distraction During Spondylolisthetic Vertebrae Restoration: A Retrospective Study.

Pain Ther 2021 Sep 15. Epub 2021 Sep 15.

Department of Spine Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.

Introduction: Based on the results of long-term clinical and radiological follow-up studies of decompression and fusion with internal fixation for degenerative lumbar spondylolisthesis (DLS), we recognized that the direction of the pedicle screw affects the stability of the fixation. However, few studies have analyzed the role of pedicle screw insertion trajectory in disc height recovery after fusion. We therefore analyzed patients' general information, clinical efficacy and sagittal, coronal and implant parameters to determine whether there is a correlation between the insertion trajectory of screws and the recovery of intervertebral space height, with the ultimate aim to provide a basis for improving the clinical efficacy and radiological outcomes of patients with DLS and to identify an optimal technique for spine surgeons that would benefit patients with spondylolisthesis.

Methods: From May 2015 to October 2019, patients who underwent single-segment decompression and fusion with internal fixation for DLS at our department were screened for enrollment in the study. The clinical history, pre- and post-operative lumbar sagittal parameters, intervertebral height, rate of recovery from spondylolisthesis and pedicle screw angle of inpatients were recorded and followed up for at least 6 months. Clinical assessments included the Oswestry Disability Index (ODI) and the Visual Analogue Scale (VAS) for lower back and leg pain. Data on screw angle, fusion segment intervertebral space height and clinical outcome were the primary outputs. Pearson correlation and multivariate regression analyses were performed to investigate the relationship between the pedicle screw angle, the sagittal parameters of the fusion segment and clinical efficacy.

Results: A total of 50 patients were initially enrolled, two patients were lost to follow-up after 6 months, 48 patients (17 men, 31 women) were eventually enrolled, and the follow-up rate was 96%. At least 6 months after the operation, vertebral spondylolisthesis improved to varying degrees [> 80% in 17 cases (35.4%) and > 20% in 43 cases (87.5%), respectively]. Changes in disc height (DH) were significantly associated with lower pedicle screw angle, while lumbar lordosis and segment lordosis remained the same. Multivariate regression analysis showed a significant negative correlation between the upper and lower pedicle screw angles and the change in DH (P < 0.05). At 2 weeks post-operation, the VAS score for low back pain and the ODI had improved significantly compared to pre-operation (P < 0.05).

Conclusions: These results suggest that the Caudad insertion trajectory technique of pedicle screws may be an ideal alternative for the treatment of DLS.

Trial Registration Number: Chinese Clinical Trial Registry (ChiCTR): ChiCTR1800020368.
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http://dx.doi.org/10.1007/s40122-021-00315-3DOI Listing
September 2021

Effect of Root Canal Therapy Combined with Full Crown Restoration on the Level of Inflammatory Factors and Chewing Function in Patients with Cracked Teeth and Chronic Pulpitis.

Evid Based Complement Alternat Med 2021 30;2021:3299349. Epub 2021 Aug 30.

Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Huangpu, Shanghai 200011, China.

Objective: To explore the clinical effect of root canal therapy combined with full crown restoration in patients with cracked teeth and chronic pulpitis.

Methods: From May 2018 to June 2020, 87 patients with cracked teeth and chronic pulpitis in our hospital were selected; the patients were randomly divided into the control group and the research group by random number method. The control group only used root canal therapy; the research group used root canal therapy combined with full crown restoration. The therapeutic effect, levels of inflammatory factors, chewing function, periodontal index, complications, and quality of life were compared between the two groups.

Results: The total effective rate of the research group (97.78%) was better than the total effective rate of the control group (85.71%) ( < 0.05). Compared with before treatment, the serum levels of interleukin-1 (IL-1), IL-6, and C-reactive protein (CRP) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the serum levels of IL-1, IL-6, and CRP in the research group decreased ( < 0.05). Compared with before treatment, the bite force of teeth and chewing efficiency of the two groups of patients increased after treatment. After treatment, compared with the control group, the bite force of teeth and chewing efficiency of the research group increased ( < 0.05). Compared with before treatment, the plaque index (PLI), probing depth (PD), gingival sulcus bleeding index (BI), and gingival index (GI) of the two groups of patients decreased after treatment. After treatment, compared with the control group, the PLI, PD, BI, and GI of the research group decreased ( < 0.05). The total incidence of complications in the research group was (11.11%), and the total incidence of complications in the control group was (16.67%); there was no significant difference between the two groups ( > 0.05). After treatment, compared with the control group, the quality of life scores of the patients in the research group were reduced ( < 0.05).

Conclusion: Root canal therapy and full crown restoration have a definite curative effect in patients with cracked teeth and chronic pulpitis, which can improve the inflammatory response, restore chewing function, maintain periodontal health, improve the quality of life, and do not increase the incidence of complications, so it has good application value.
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http://dx.doi.org/10.1155/2021/3299349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423566PMC
August 2021

Fecal Bacteria as Non-Invasive Biomarkers for Colorectal Adenocarcinoma.

Front Oncol 2021 10;11:664321. Epub 2021 Aug 10.

Department of Gastroenterological Surgery, Shanghai East Hospital, Tongji University of Medicine, Shanghai, China.

Colorectal adenocarcinoma (CRC) ranks one of the five most lethal malignant tumors both in China and worldwide. Early diagnosis and treatment of CRC could substantially increase the survival rate. Emerging evidence has revealed the importance of gut microbiome on CRC, thus fecal microbial community could be termed as a potential screen for non-invasive diagnosis. Importantly, few numbers of bacteria genus as non-invasive biomarkers with high sensitivity and specificity causing less cost would be benefitted more in clinical compared with the whole microbial community analysis. Here we analyzed the gut microbiome between CRC patients and healthy people using 16s rRNA sequencing showing the divergence of microbial composition between case and control. Furthermore, ExtraTrees classifier was performed for the classification of CRC gut microbiome and heathy control, and 13 bacteria were screened as biomarkers for CRC. In addition, 13 biomarkers including 12 bacteria genera and FOBT showed an outstanding sensitivity and specificity for discrimination of CRC patients from healthy controls. This method could be used as a non-invasive method for CRC early diagnosis.
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http://dx.doi.org/10.3389/fonc.2021.664321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383742PMC
August 2021

Pollution alters methanogenic and methanotrophic communities and increases dissolved methane in small ponds.

Sci Total Environ 2021 Aug 18;801:149723. Epub 2021 Aug 18.

Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China. Electronic address:

Small ponds have become a hotspot of greenhouse gas emissions, but our understanding of methane (CH) cycling and its biological regulation in small polluted ponds remains limited. To assess how pollution affects CH content, we investigated dissolved CH concentrations, water and sediments properties, methanogenic and methanotrophic communities in two types of small polluted ponds. Compared with low pollution (LP) ponds, high pollution (HP) ponds showed significantly (P < 0.05) higher dissolved CH in water. Sequencing of methyl coenzyme M reductase (mcrA) and particulate methane monooxygenase (pmoA) genes showed that HP led to significant (P < 0.05) shifts of CH-cycling microbial communities, with increased Shannon index of sediment methanogenic communities and water methanotrophic communities. There were also strong negative associations (P < 0.05) between dissolved CH concentrations and interdomain methanogen-methanotroph network connectivity in water and sediments, respectively. The partial least squares path modeling indicated that dissolved oxygen, total organic carbon, ammonium nitrogen and nitrate nitrogen of water, and total nitrogen and total carbon of sediment, and CH-cycling microbes could regulate the CH content. This study clarified the effects of environmental deterioration on CH cycling in small ponds, highlighting the use of methanogen-methanotroph network connectivity to assess the CH production.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149723DOI Listing
August 2021

Clinical Epidemiology in China series. Paper 1: Evidence-based medicine in China: An oral history study.

J Clin Epidemiol 2021 Aug 17. Epub 2021 Aug 17.

Evidence-Based Medicine Centre, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China; Lanzhou University Institute of Health Data Science, Lanzhou 730000, China; Lanzhou University GRADE Centre, Lanzhou 730000, China; World Health Organization (WHO) Collaborating Centre for Guideline Implementation and Knowledge Translation, Lanzhou 730000, China. Electronic address:

Objective: To describe and record the evolution of EBM in China.

Study Design And Setting: We conducted the study following the general methodology of oral history. The interviews were done at Lanzhou University, between 18 and 22 April 2019 using pre-defined questions. All interviews were videorecorded. Two investigators extracted and analyzed the information from the interviews independently.

Results: One international expert and ten Chinese experts participated in the interviews. After the introduction of EBM in China in the mid-1990s, more than 20 EBM centres have been established. According to the interviewees, Gordon Guyatt, David Sackett and Iain Chalmers are the international experts who played the most important role in the development of EBM in China. China has contributed to EBM on the international level by conducting systematic reviews, developing reporting checklists, and introducing the principles of EBM into Traditional Medicine. The Chinese Cochrane Centre and the EBM Centre of Lanzhou University were ranked the top two EBM Centres in China by the interviewees.

Conclusion: EBM has been developing in China for nearly a quarter of a century. Many achievements have been reached, however, EBM is still facing many challenges in China, including shortages of funding support and personnel, as well as limited local high-quality evidence.
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http://dx.doi.org/10.1016/j.jclinepi.2021.08.012DOI Listing
August 2021

Anti-Golgi Antibody as a Potential Indicator for Rheumatoid Arthritis.

Lab Med 2021 Aug 20. Epub 2021 Aug 20.

West China School of Medicine/Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.

Objective: To reveal the relationship between anti-Golgi antibody (AGA) and clinical diseases through retrospective analysis.

Methods: The clinical data of 584 cases testing positive for AGA in the past 11 years were collected and retrospectively analyzed.

Results: AGA pattern accounted for .2% of positive ANA results. In total, 35.0% of diagnosed patients had autoimmune diseases (AID), mainly rheumatoid arthritis (RA). High-titer AGA (≧1:1000) was common in AID. In nondiagnosed patients with clinical symptoms, joint pain/muscle pain was the most common.

Conclusions: Positive AGA with high titer was closely related to RA. Joint pain/muscle pain was the most common symptom in patients who tested AGA positive. Therefore, AGA may be a key indicator of RA in the Chinese population.
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http://dx.doi.org/10.1093/labmed/lmab046DOI Listing
August 2021

Achieving advanced nitrogen removal in a novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process treating low C/N ratio municipal wastewater.

Bioresour Technol 2021 Nov 30;340:125661. Epub 2021 Jul 30.

Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, School of Ecological and Environmental Science, Hainan University, Haikou 570228, China. Electronic address:

For achieving mainstream anammox, a novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process to treat municipal wastewater was developed. This process achieved a total inorganic nitrogen (TIN) removal efficiency of 81%, with an average effluent TIN of 7.31 mg·L, when the ratio of influent chemical oxygen demand (COD) to TIN was 3.2. Approximately 97% of the TIN was removed by anammox in the PDA biofilter. Nitrite was provided by partial denitrification for anammox. Partial denitrification was driven by Thaurea in the middle and lower regions of the PDA biofilter, while anammox was mainly driven by Candidatus Brocadia in the middle and upper regions. When treating real municipal wastewater, the TIN was efficiently removed in the PDA-N biofilter, with the effluent TIN of 5.96 mg·L. Anammox played a primary role, achieving approximately 98% of the TIN removal. Compared to the traditional nitrification/denitrification process, this process can economize organic carbon demand and oxygen consumption.
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http://dx.doi.org/10.1016/j.biortech.2021.125661DOI Listing
November 2021

Understanding the effect of free ammonia on microbial nitrification mechanisms in suspended activated sludge bioreactors.

Environ Res 2021 09 22;200:111737. Epub 2021 Jul 22.

School of Environmental and Material Engineering, Yantai University, Yantai, 264005, China. Electronic address:

During nitrification, the varieties of microbial structures, metabolic pathways and functional profiles in four parallel laboratory-scale sequencing batch reactors (SBRs) with 0.5, 5, 10 and 15 mg/L of free ammonia (FA) concentrations were analyzed by high-throughput sequencing of the 16S rRNA gene. The SBRs were named S, S, S and S, respectively. Ammonia removal via the nitrate pathway was achieved in S and S throughout the whole experimental period, while ammonia removal via the nitrite pathway was established in S and S after 89 and 146 day, respectively. The key finding of this study is that both the microbial diversity and richness were significantly affected (p < 0.05) by the FA concentration at different taxonomic levels. The most dominant taxa of S, S and S were same, and mainly included Thauera while S was mainly composed of Zoogloea. Linear discriminant analysis (LDA) effect size (LEfSe) analysis was used to identify unique biomarkers in SBR activated sludge (AS) sample. The functional genera and enzyme in the four SBRs are similar but different in abundance and they are responsible for the removal of organics and nitrogen. Moreover, metabolic pathways are similar by PICRUSt analysis. The relative proportions of pathway-specific genes involved in some metabolic pathways differed to some extent. The ammonia oxidation rate was positively linked to Nitrosomonas and amo (both Spearman correlation coefficients (ρ) = 0.777) while the nitrite oxidation rate was positively linked to Nitrospira (ρ = 0.777) by co-occurrence network analysis. This work deciphered the response of microbial characteristics to different FA constraints in AS process and could provide helpful information for revealing the biological mechanism of FA inhibition on nitrogen removal.
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http://dx.doi.org/10.1016/j.envres.2021.111737DOI Listing
September 2021

Study on a Novel Biodegradable and Antibacterial Fe-Based Alloy Prepared by Microwave Sintering.

Materials (Basel) 2021 Jul 6;14(14). Epub 2021 Jul 6.

School of Mechanical and Mining Engineering, The University of Queensland, Brisbane, QLD 4072, Australia.

This research produced a porous Fe-8 wt.% Cu alloy by microwave sintering in order to achieve (i) an increased biodegradation rate, and (ii) an antibacterial function. The Fe-8Cu alloy had higher density, hardness and degradation rate (about 2 times higher) but smaller and fewer surface pores, compared to the pure Fe. The Fe-8Cu alloy had a strong antibacterial function (the antibacterial rates against were up to 99.9%) and good biocompatibility. This work provides a novel approach of alloy design and processing to develop novel antibacterial Fe-based alloys.
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http://dx.doi.org/10.3390/ma14143784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303899PMC
July 2021

Do the positioning variables of the cage contribute to adjacent facet joint degeneration? Radiological and clinical analysis following intervertebral fusion.

Ann Transl Med 2021 May;9(9):776

Department of Spine Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Background: Compared to other risk factors, adjacent facet joint degeneration (AFD) is the main contributor to adjacent segment disease (ASD). The interbody cage may be a potential indirect risk of AFD. This study investigated the correlations among the lumbar sagittal balance parameters, the inter-body cage's intraoperative positioning variables, and adjacent facet joint degeneration following the transforaminal lumbar interbody fusion (TLIF) technique.

Methods: Patients who accepted single-level TLIF for symptomatic lumbar degenerative disease and were followed up for at least six months were enrolled in this study. According to the inclusive and exclusive criteria, 93 patients were included (44 males and 49 females). X-ray and computed tomography (CT) images were obtained before and six months after surgery. The vertebral contour and the center of the marker mass in the cage were calculated using a geometric algorithm. Orthopedic surgeons measured the disc height, lordosis angle, and facet joint degeneration. Patient-reported outcomes, including the Oswestry Disability Index (ODI) and the visual analog scale (VAS), were used to assess the clinical outcomes. The Student's t-test, Wilcoxon rank-sum test, and Chi-square test were used for the statistical analyses.

Results: The average age was 53.7 years old (range, 27-84 years). The average functional disability outcome assessed by the ODI was 61.2, and the average back and leg pain assessed by the VAS was 6.2 and 6.9, respectively. The patients were categorized into a normal group and an abnormal (AFD) group according to whether the facet joint degeneration was aggravated. The abnormal group had a higher back pain VAS score (P=0.031) and lower sagittal vertical position (P=0.027). The other parameters were similar at baseline (P>0.05). The cage's sagittal vertical position decreased significantly with AFD aggravation (OR, 0.737; 95% CI, 0.561-0.969).

Conclusions: In patients with AFD aggravation, the preoperative VAS and postoperative ODI scores were significantly higher. The cage position parameters were related to AFD. A lower cage center was associated with a greater incidence of AFD.
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http://dx.doi.org/10.21037/atm-20-7718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246202PMC
May 2021

Discovery of Potent Selective Nonzinc Binding Autotaxin Inhibitor BIO-32546.

ACS Med Chem Lett 2021 Jul 14;12(7):1124-1129. Epub 2021 Jun 14.

Medicinal Chemistry, Physical Biochemistry, Drug Metabolism & Pharmacokinetics, Discovery Bioassay, Neurology, Biogen Inc., 225 Binney St, Cambridge, Massachusetts 02142, United States.

Autotaxin (ATX) is a lysophospholipase D that is the main enzyme responsible for generating LPA in body fluids. Although ATX was isolated from a conditioned medium of melanoma cells, later it was discovered to play a critical role in vascular and neuronal development. ATX has also been implicated in primary brain tumor, fibrosis, and rheumatoid arthritis, as well as neurological diseases such as multiple sclerosis, Alzheimer's disease, and neuropathic pain. As ATX and LPA levels are increased upon neuronal injury, a selective ATX inhibitor could provide a new approach to treat neuropathic pain. Herein we describe the discovery of a novel series of nonzinc binding reversible ATX inhibitors, particularly a potent, selective, orally bioavailable, brain-penetrable tool compound BIO-32546, as well as its synthesis, X-ray cocrystal structure, pharmacokinetics, and in vivo efficacy.
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http://dx.doi.org/10.1021/acsmedchemlett.1c00211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274069PMC
July 2021

Components and Functional Diversification of Florigen Activation Complexes in Cotton.

Plant Cell Physiol 2021 Jul 10. Epub 2021 Jul 10.

College of Life Sciences, Shihezi University, Shihezi 832003, China.

In shoot apex cells of rice, a hexameric florigen activation complex (FAC), comprising FLOWERING LOCUS T (FT), 14-3-3 and the bZIP transcription factor FD, activates downstream target genes and regulates several developmental transitions, including flowering. The allotetraploid cotton (Gossypium hirsutum L.) contains only one FT locus in both of the A- and D-subgenomes. However, there is limited information regarding cotton FACs. Here, we identified a 14-3-3 protein that interacts strongly with GhFT in the cytoplasm and nuclei, and five FD homoeologous gene pairs were characterized. In vivo, all five GhFD proteins interacted with Gh14-3-3 and GhFT in the nucleus. GhFT, 14-3-3, and all the GhFDs interacted in the nucleus as well, suggesting that they formed a ternary complex. Virus-induced silencing of GhFD1, -2, and -4 in cotton delayed flowering and inhibited the expression of floral meristem identity genes. Silencing GhFD3 strongly decreased lateral root formation, suggesting a function in lateral root development. GhFD overexpression in Arabidopsis and transcriptional activation assays suggested that FACs containing GhFD1 and GhFD2 function mainly in promoting flowering with partial functional redundancy. Moreover, GhFD3 was specifically expressed in lateral root meristems and dominantly activated the transcription of AUXIN RESPONSE FACTOR genes, such as ARF19. Thus, the diverse functions of FACs may depend on the recruited GhFD. Creating targeted genetic mutations in the florigen system using CRISPR-Cas genome editing may fine-tune flowering and improve plant architecture.
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http://dx.doi.org/10.1093/pcp/pcab107DOI Listing
July 2021

Mass Spectrometry Provides a Highly Sensitive Noninvasive Means of Sequencing and Tracking M-Protein in the Blood of Multiple Myeloma Patients.

J Proteome Res 2021 08 9;20(8):4176-4185. Epub 2021 Jul 9.

University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

The amino acid sequence of the M-protein for multiple myeloma is unique compared to the polyclonal antibodies in patients' blood. This uniqueness is exploited to develop an ultrasensitive M-protein detection method utilizing mass spectrometry (MS). The method involves the de novo amino acid sequencing of the full-length M-protein, and a targeted MS/MS assay to detect and quantify the unique M-protein sequence in serum samples. Healthy control serum spiked with NISTmAb and serial samples from an MM patient were used to demonstrate the ability of the platform to sequence and monitor a target M-protein. The de novo NISTmAb protein sequence obtained matched the published sequence, confirming the ability of the platform to accurately sequence a target M-protein in serum. NISTmAb was quantified down to 0.0002 g/dL in serum, a level hundreds of times more sensitive than conventional blood-based tests such as SPEP and IFE. The M-protein in the patient sample could be quantified throughout complete remission, demonstrating the utility of the assay to track M-protein considerably beyond the sensitivities of current blood-based tests. Notably, the assay detected a 2-fold rise in M-protein levels 10 months before any changes were detected by conventional IFE. The MS-based assay is highly sensitive, noninvasive, and requires only a small amount of serum, less than 100 μL. Sequencing data is deposited into PRIDE with identifier PXD022784, and quantification data can be found in Panorama Public with identifier PXD022980.
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http://dx.doi.org/10.1021/acs.jproteome.0c01022DOI Listing
August 2021

Global prevalence of sarcopenic obesity in older adults: A systematic review and meta-analysis.

Clin Nutr 2021 07 21;40(7):4633-4641. Epub 2021 Jun 21.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Evidence-based Nursing Center, School of Nursing, Lanzhou University, Lanzhou, China; Key Laboratory of Evidence-Based Medicine and Knowledge Translation of Gansu Province, Lanzhou, China. Electronic address:

Background & Aims: Sarcopenic obesity (SO), which refers to the coexistence of sarcopenia and obesity. It can lead to physical disability, morbidity, and even mortality. This systematic review and meta-analysis aimed to estimate the global prevalence of SO in older adults.

Methods: We searched PubMed, Embase, and Web of Science for studies reporting the prevalence of SO from inception to December 2020. Two researchers independently screened the literature, evaluated study quality, and extracted data. A random-effects model was used to pool the estimates for the prevalence of SO. Subgroup analysis, sensitivity analysis, and meta-regression analysis were conducted. Publication bias was assessed using a funnel plot and the Egger test. All statistical analyses were performed using Stata 15.0 software.

Results: This review included 50 studies, we found that the global prevalence of SO in older adults was 11%. Subgroup analyses showed that the prevalence of SO was higher among studies using diagnostic criteria of muscle mass alone (15%) to diagnose sarcopenia, using dual-energy X-ray absorptiometry (15%) to assess muscle mass, and those focused on age ≥ 75 years old (23%), hospitalized (16%), South Americans (21%) and North Americans (19%). There were no significant differences in the prevalence of SO among studies using body fat percentage (10%), body mass index (13%), waist circumference (16%) to diagnose obesity and in female (14%), male (10%) patients. Sensitivity analysis showed that none of the studies affected the overall pooled results. Meta-regression analysis found that publication year, geographical region, study setting, and the diagnostic criteria of sarcopenia were sources of heterogeneity.

Conclusion: This meta-analysis indicated SO affects more than one in ten older adults globally. Therefore, we should attach importance to the screening and early diagnosis of SO in older adults, then selecting appropriate interventions to reduce the occurrence of it and various adverse outcomes in this demographic.
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http://dx.doi.org/10.1016/j.clnu.2021.06.009DOI Listing
July 2021

A Personalized Mass Spectrometry-Based Assay to Monitor M-Protein in Patients with Multiple Myeloma (EasyM).

Clin Cancer Res 2021 Sep 1;27(18):5028-5037. Epub 2021 Jul 1.

Princess Margaret Cancer Centre, Toronto, Ontario, Canada.

Purpose: M-protein is a well-established biomarker used for multiple myeloma monitoring. Current improvements in multiple myeloma treatment created the need to monitor minimal residual disease (MRD) with high sensitivity. Measuring residual levels of M-protein in serum by MS was established as a sensitive assay for disease monitoring. In this study we evaluated the performance of EasyM-a noninvasive, sensitive, MS-based assay for M-protein monitoring.

Experimental Design: Twenty-six patients enrolled in MCRN-001 clinical trial of two high-dose alkylating agents as conditioning followed by lenalidomide maintenance were selected for the study. All selected patients achieved complete responses (CR) during treatment, whereas five experienced progressive disease on study. The M-protein of each patient was first sequenced from the diagnostic serum using our protein sequencing platform. The patient-specific M-protein peptides were then measured by targeted MS assay to monitor the response to treatment.

Results: The M-protein doubling over 6 months measured by EasyM could predict the relapse in 4 of 5 relapsed patients 2 to 11 months earlier than conventional testing. In 21 disease-free patients, the M-protein was still detectable by EasyM despite normal FLC and MRD negativity. Importantly, of 72 MRD negative samples with CR status, 62 were positive by EasyM. The best sensitivity achieved by EasyM, detecting 0.58 mg/L of M-protein, was 1,000- and 200-fold higher compared with serum protein electrophoresis and immunofixation electrophoresis, respectively.

Conclusions: EasyM was demonstrated to be a noninvasive, sensitive assay with superior performance compared with other assays, making it ideal for multiple myeloma monitoring and relapse prediction.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-0649DOI Listing
September 2021

Geometric resistant polar quaternion discrete Fourier transform and its application in color image zero-hiding.

ISA Trans 2021 Jun 18. Epub 2021 Jun 18.

Department of Biomedical Data Science, Stanford University, Stanford, CA 94305, USA.

As a typical frequency-domain analysis method, quaternion discrete Fourier transform (QDFT) has been widely used in information hiding in color images. However, due to the sensitivity of QDFT to geometric attacks, existing QDFT-based information hiding schemes have limited ability in resisting geometric attacks. In this study, a kind of novel geometrically resilient polar QDFT (PQDFT) is constructed and the properties of the proposed PQDFT are analyzed. Subsequently, a PQDFT-based color image zero-hiding scheme robust to geometric attacks is proposed for lossless copyright protection of color images, which experimentally shows reasonable resistance against geometric and common attacks, indicating better robustness compared with the existing QDFT-based information hiding schemes and other leading-edge zero-hiding schemes.
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http://dx.doi.org/10.1016/j.isatra.2021.06.019DOI Listing
June 2021

Local anesthetic levobupivacaine induces ferroptosis and inhibits progression by up-regulating p53 in non-small cell lung cancer.

Aging (Albany NY) 2021 Jun 27;13. Epub 2021 Jun 27.

Department of Anesthesiology, Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 271199, China.

Non-small cell lung cancer (NSCLC) is a common malignancy with high mortality and poor prognosis. Levobupivacaine is a widely used local anesthetic and presents potential anti-tumor activity. Nevertheless, the function of levobupivacaine in the NSCLC development remains elusive. Here, we tried to investigate the impact of levobupivacaine on the NSCLC progression and the underlying mechanism. Significantly, we revealed that levobupivacaine could inhibit the proliferation and induce the apoptosis of NSCLC cells. Levobupivacaine was able to attenuate the invasion and migration in the cells. Meanwhile, the treatment of levobupivacaine enhanced the erastin-induced inhibition of cell growth of NSCLC cells. The treatment of levobupivacaine remarkably increased the levels of ROS, iron, and Fe2+ in NSCLC cells. Mechanically, levobupivacaine up-regulated the expression of p53 and induced ferroptosis by regulating p53 in NSCLC cells. Moreover, tumorigenicity analysis in nude mice showed that the treatment of levobupivacaine significantly repressed the tumor growth of NSCLC cells . In summary, we concluded that the local anesthetic levobupivacaine inhibits the progression and induces ferroptosis of NSCLC by up-regulating p53. Our finding provides new insights into the mechanism by which levobupivacaine attenuates the development of NSCLC. Levobupivacaine may serve as a potential anti-tumor candidate for the therapeutic strategy of NSCLC.
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http://dx.doi.org/10.18632/aging.203138DOI Listing
June 2021

Retroperitoneal versus transperitoneal approach for elective open abdominal aortic aneurysm repair.

Cochrane Database Syst Rev 2021 06 21;6:CD010373. Epub 2021 Jun 21.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou City, China.

Background: There has been extensive debate in the surgical literature regarding the optimum surgical access approach to the infrarenal abdominal aorta during an operation to repair an abdominal aortic aneurysm. The published trials comparing retroperitoneal (RP) and transperitoneal (TP) aortic surgery show conflicting results. This is an update of the review first published in 2016.

Objectives: To assess the effectiveness and safety of the retroperitoneal versus transperitoneal approach for elective open abdominal aortic aneurysm repair on mortality, complications, hospital stay and blood loss.

Search Methods: The Cochrane Vascular Information Specialist searched the Cochrane Vascular Specialised Register, CENTRAL, MEDLINE, Embase and CINAHL databases and the World Health Organization International Clinical Trials Registry Platform and the ClinicalTrials.gov trials registers to 30 November 2020. The review authors searched the Chinese Biomedical Literature Database and handsearched reference lists of relevant articles to identify additional trials.

Selection Criteria: We included randomized controlled trials (RCTs) that assessed the RP approach versus the TP approach for elective open abdominal aortic aneurysm (AAA) repair. There were no restrictions on language or publication status.

Data Collection And Analysis: Two review authors independently extracted data from the included trials. We resolved any disagreements through discussion with a third review author. Two review authors independently assessed the risk of bias in included trials with the Cochrane risk of bias tool. For dichotomous outcomes, we calculated the odds ratio (OR) with the corresponding 95% confidence interval (CI). For continuous data, we calculated a pooled estimate of treatment effect by calculating the mean difference (MD) and standard deviation (SD) with corresponding 95% CIs. We pooled data using a fixed-effect model, unless we identified heterogeneity, in which case we used a random-effects model. We used GRADE to assess the overall certainty of the evidence. We evaluated the outcomes of mortality, complications, intensive care unit (ICU) stay, hospital stay, blood loss, aortic cross-clamp time and operating time.

Main Results: We identified no new studies from the updated searches. After reassessment, we included one study which had previously been excluded. Five RCTs with a combined total of 152 participants are included. The overall certainty of the evidence ranged from low to very low because of the low methodological quality of the included trials (unclear random sequence generation method and allocation concealment, and no blinding of outcome assessors), small sample sizes, small number of events, high heterogeneity and inconsistency between the included trials, no power calculations and relatively short follow-up. There was no evidence of a difference between the RP approach and the TP approach regarding mortality (odds ratio (OR) 0.32, 95% CI 0.01 to 8.25; 3 studies, 110 participants; very low-certainty evidence). Similarly, there was no evidence of a difference in complications such as hematoma (OR 0.90, 95% CI 0.13 to 6.48; 2 studies, 75 participants; very low-certainty evidence), abdominal wall hernia (OR 10.76, 95% CI 0.55 to 211.78; 1 study, 48 participants; very low-certainty evidence), or chronic wound pain (OR 2.20, 95% CI 0.36 to 13.34; 1 study, 48 participants; very low-certainty evidence) between the RP and TP approaches in participants undergoing elective open AAA repair. The RP approach may reduce ICU stay (mean difference (MD) -19.02 hours, 95% CI -30.83 to -7.21; 3 studies, 106 participants; low-certainty evidence); hospital stay (MD -3.30 days, 95% CI -4.85 to-1.75; 5 studies, 152 participants; low-certainty evidence); and blood loss (MD -504.87 mL, 95% CI -779.19 to -230.56; 4 studies, 129 participants; very low-certainty evidence). There was no evidence of a difference between the RP approach and the TP approach regarding aortic cross-clamp time (MD 0.69 min, 95% CI -7.23 to 8.60; 4 studies, 129 participants; very low-certainty evidence) or operating time (MD -15.94 min, 95% CI -34.76 to 2.88; 4 studies, 129 participants; very low-certainty evidence).

Authors' Conclusions: Very low-certainty evidence from five small RCTs showed no clear evidence of a difference between the RP approach and the TP approach for elective open AAA repair in terms of mortality, or for rates of complications including hematoma (very low-certainty evidence), abdominal wall hernia (very low-certainty evidence), or chronic wound pain (very low-certainty evidence). However, a shorter intensive care unit (ICU) stay and shorter hospital stay was probably indicated following the RP approach compared to the TP approach (both low-certainty evidence). A possible reduction in blood loss was also shown after the RP approach (very low-certainty evidence). There is no clear difference between the RP approach and TP approach in aortic cross-clamp time or operating time. Further well-designed, large-scale RCTs assessing the RP approach versus TP approach for elective open AAA repair are required.
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http://dx.doi.org/10.1002/14651858.CD010373.pub3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216039PMC
June 2021

Identification of Methylation-Regulated Differentially Expressed Genes and Related Pathways in Hepatocellular Carcinoma: A Study Based on TCGA Database and Bioinformatics Analysis.

Front Oncol 2021 3;11:636093. Epub 2021 Jun 3.

Department of Internal Oncology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, China.

Background: In recent years, DNA methylation modification has been shown to be a critical mechanism in the field of epigenetics.

Methods: Hepatocellular carcinoma (HCC) data were obtained from The Cancer Genome Atlas project, including RNA expression profiles, Illumina Human Methylation 450K BeadChip data, clinical information, and pathological features. Then, differentially expressed genes (DEGs) and differentially methylated genes were identified using R software. Methylation-regulated DEGs (MeDEGs) were further analyzed using Spearman's correlation analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID database and ClueGO in Cytoscape software. Kaplan-Meier survival analysis explored the relationship between methylation, expression of MeDEGs, and survival time. Gene set enrichment analysis (GSEA) was conducted to predict the function of prognosis-related MeDEGs.

Results: A total of nine up-regulated and 72 down-regulated MeDEGs were identified. GO and KEGG pathway analyses results indicated that multiple cancer-related terms were enriched. Kaplan-Meier survival analysis showed that the methylation status of four MeDEGs (CTF1, FZD8, PDK4, and ZNF334) was negatively associated with overall survival. Moreover, the methylation status of CDF1 and PDK4 was identified as an independent prognostic factor. According to GSEA, hypermethylation of prognosis-related MeDEGs was enriched in pathways that included "Spliceosome", "Cell cycle", "RNA degradation", "RNA polymerase", "DNA replication", "Mismatch repair", "Base excision repair", "Nucleotide excision repair", "Homologous recombination", "Protein export", and "Pyrimidine metabolism".

Conclusions: Aberrant DNA methylation plays a critical role in malignant progression of HCC. Prognosis-related MeDEGs identified in this research may be potential biomarkers and targets in diagnosis and treatment.
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http://dx.doi.org/10.3389/fonc.2021.636093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209385PMC
June 2021

Occurrence, accumulation, and risk assessment of trace metals in tea (Camellia sinensis): A national reconnaissance.

Sci Total Environ 2021 Oct 9;792:148354. Epub 2021 Jun 9.

Department of Tea Science, Zhejiang University, Hangzhou 310058, China. Electronic address:

Accumulation of potentially toxic elements in soil and tea leaves is a particular concern for tea consumers worldwide. However, the contents of potentially toxic elements and their potential health and ecological risks in Chinese tea gardens have rarely been investigated on the national scale. In this study, we collected 225 paired soil and tea plant samples from 45 tea gardens in 15 provinces of China to survey the current risk of potentially toxic element accumulation in Chinese tea gardens. The results suggest that the average contents of most trace metals in rhizosphere soils meet the risk control standard for agricultural land in China. However, the mean contents of As, Cr, Cd, Zn, Cu, and Ni in rhizosphere soils were 1.94, 2.14, 1.23, 1.15, 1.18, and 1.19 times their corresponding background soil values in China. Cd had the highest geo-accumulation index, followed by As, Zn, Cr, Ni, Cu, Pb, and Mn in rhizosphere soils. Nearly 2.22% and 4.44% of soils were moderately to heavily contaminated with As and Cd, respectively. The risk index ranged from 18.0 to 292, with an average value of 90.0, indicating low to moderate ecological risk in Chinese tea gardens. This is the first national-scale reconnaissance of trace metals in tea across China. Our findings provide a useful reference for ensuring the quality and safety of tea production and mitigating the risk of toxic element accumulation in tea.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148354DOI Listing
October 2021

Next-generation Bruton's tyrosine kinase inhibitor BIIB091 selectively and potently inhibits B cell and Fc receptor signaling and downstream functions in B cells and myeloid cells.

Clin Transl Immunology 2021 14;10(6):e1295. Epub 2021 Jun 14.

MS Development Unit Biogen Cambridge MA USA.

Objectives: Bruton's tyrosine kinase (BTK) plays a non-redundant signaling role downstream of the B-cell receptor (BCR) in B cells and the receptors for the Fc region of immunoglobulins (FcR) in myeloid cells. Here, we characterise BIIB091, a novel, potent, selective and reversible small-molecule inhibitor of BTK.

Methods: BIIB091 was evaluated and in preclinical models and in phase 1 clinical trial.

Results: BIIB091 potently inhibited BTK-dependent proximal signaling and distal functional responses in both B cells and myeloid cells with ICs ranging from 3 to 106 nm, including antigen presentation to T cells, a key mechanism of action thought to be underlying the efficacy of B cell-targeted therapeutics in multiple sclerosis. BIIB091 effectively sequestered tyrosine 551 in the kinase pocket by forming long-lived complexes with BTK with of more than 40 min, thereby preventing its phosphorylation by upstream kinases. As a key differentiating feature of BIIB091, this property explains the very potent whole blood ICs of 87 and 106 nm observed with stimulated B cells and myeloid cells, respectively. , BIIB091 blocked B-cell activation, antibody production and germinal center differentiation. In phase 1 healthy volunteer trial, BIIB091 inhibited naïve and unswitched memory B-cell activation, with an IC of 55 nm and without significant impact on lymphoid or myeloid cell survival after 14 days of dosing.

Conclusion: Pharmacodynamic results obtained in preclinical and early clinical settings support the advancement of BIIB091 in phase 2 clinical trials.
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http://dx.doi.org/10.1002/cti2.1295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204096PMC
June 2021

MSTracer: A Machine Learning Software Tool for Peptide Feature Detection from Liquid Chromatography-Mass Spectrometry Data.

J Proteome Res 2021 07 17;20(7):3455-3462. Epub 2021 Jun 17.

Cheriton School of Computer Science, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

Liquid chromatography with tandem mass spectrometry (MS/MS) has been widely used in proteomics. Although a typical experiment includes both MS and MS/MS scans, existing bioinformatics research has focused far more on MS/MS data than on MS data. In MS data, each peptide produces a few trails of signal peaks, which are collectively called a peptide feature. Here, we introduce MSTracer, a new software tool for detecting peptide features from MS data. The software incorporates two scoring functions based on machine learning: one for detecting the peptide features and the other for assigning a quality score to each detected feature. The software was compared with several existing tools and demonstrated significantly better performance.
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http://dx.doi.org/10.1021/acs.jproteome.0c01029DOI Listing
July 2021

Key Metabolic Enzymes Involved in Remdesivir Activation in Human Lung Cells.

Antimicrob Agents Chemother 2021 08 17;65(9):e0060221. Epub 2021 Aug 17.

Gilead Sciences, Inc., Foster City, California, USA.

Remdesivir (RDV; GS-5734, Veklury), the first FDA-approved antiviral to treat COVID-19, is a single-diastereomer monophosphoramidate prodrug of an adenosine analogue. RDV is taken up in the target cells and metabolized in multiple steps to form the active nucleoside triphosphate (TP) (GS-443902), which, in turn, acts as a potent and selective inhibitor of multiple viral RNA polymerases. In this report, we profiled the key enzymes involved in the RDV metabolic pathway with multiple parallel approaches: (i) bioinformatic analysis of nucleoside/nucleotide metabolic enzyme mRNA expression using public human tissue and lung single-cell bulk mRNA sequence (RNA-seq) data sets, (ii) protein and mRNA quantification of enzymes in human lung tissue and primary lung cells, (iii) biochemical studies on the catalytic rate of key enzymes, (iv) effects of specific enzyme inhibitors on the GS-443902 formation, and (v) the effects of these inhibitors on RDV antiviral activity against SARS-CoV-2 in cell culture. Our data collectively demonstrated that carboxylesterase 1 (CES1) and cathepsin A (CatA) are enzymes involved in hydrolyzing RDV to its alanine intermediate MetX, which is further hydrolyzed to the monophosphate form by histidine triad nucleotide-binding protein 1 (HINT1). The monophosphate is then consecutively phosphorylated to diphosphate and triphosphate by cellular phosphotransferases. Our data support the hypothesis that the unique properties of RDV prodrug not only allow lung-specific accumulation critical for the treatment of respiratory viral infection such as COVID-19 but also enable efficient intracellular metabolism of RDV and its MetX to monophosphate and successive phosphorylation to form the active TP in disease-relevant cells.
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http://dx.doi.org/10.1128/AAC.00602-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370248PMC
August 2021

N--Feruloyloctopamine Wakes Up BBC3, DDIT3, CDKN1A, and NOXA Signals to Accelerate HCC Cell Apoptosis.

Anal Cell Pathol (Amst) 2021 22;2021:1560307. Epub 2021 May 22.

General Surgery Department, The First Hospital of Lanzhou University, The First Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China.

N--Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. However, the specific targets and detailed mechanisms still need to be clarified. Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. Real-time cell analyzer and flow cytometry data reveal that tumor cell proliferation and apoptosis were impacted by FO. DAVID bioinformatic data showed that most of the biological process GO terms are related to proliferation and apoptosis. KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. The result implied that FO might exacerbate HCC cell apoptosis by regulating BBC3, DDIT3, CDKN1A, and NOXA signals. The obstacle effect of FO can provide new targets and new credibility for the treatment of liver cancer.
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http://dx.doi.org/10.1155/2021/1560307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166497PMC
May 2021

Insight into the adsorption of europium(III) on muscovite and phlogopite: Effects of pH, electrolytes, humic substances and mica structures.

Chemosphere 2021 Nov 5;282:131087. Epub 2021 Jun 5.

Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University, Zhuhai, 519082, China. Electronic address:

Europium(III), i.e., Eu(III), is chemically analogous to the trivalent lanthanides (Ln) and actinides (An). A good understanding of the adsorption behaviour of Eu(III) on mica group minerals is critical to the safety evaluation of the radioactive contamination. Nevertheless, the structural complexity of micaceous minerals makes it difficult to draw a consistent conclusion in the study of Eu(III) migration. In this work, we contrastively studied Eu(III) adsorption on dioctahedral muscovite and trioctahedral phlogopite as functions of pH, ionic strength, background electrolytes, interaction sequence, and fulvic acid (FA). Batch experiments showed that Eu(III) adsorption on both micas was strongly dependent on pH but quite independent on ionic strength that is determined by Na. Planar sites are available on both muscovite and phlogopite while interlayer sites only on phlogopite under Na and Ca electrolytes (not for K and Cs). An interlayer expansion of phlogopite, as indicated by a newly appeared diffraction peak at ~6° 2-theta, occurred along with Eu(III) adsorption, which was also confirmed by transmission electron microscopy. Furthermore, the initial Eu(III) concentrations, the concentration ratios between Eu(III) and Cs, and the reaction sequences of Eu(III)-electrolytes-FA affected both the adsorption behaviour of Eu(III) and reversely the structural alteration of phlogopite. The sequential extraction showed that the adsorbed Eu(III) was mainly in the ion-exchangeable form while the addition of FA could increase the portion of coordinative species. The currently proposed Eu(III) adsorption mechanism can shed new light on predicting the migration of Ln/An(III) at the mica-rich solid-liquid interface on a molecular scale.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131087DOI Listing
November 2021

HfSiO Nanocomposite Coatings Prepared by Ion-Assisted Co-Evaporation Process for Low-Loss and High-LIDT Optics.

Materials (Basel) 2021 May 17;14(10). Epub 2021 May 17.

Key Laboratory of Advanced Micro-Structured Materials, Ministry of Education, Shanghai 200092, China.

HfSiO nanocomposites with different SiO doping ratios were synthesized using an ion-assisted co-evaporation process to achieve dense amorphous HfSiO coatings with low loss and a high laser-induced damage threshold (LIDT). The results showed that the HfSiO nanocomposites (x ≥ 0.20) exhibited excellent comprehensive performance with a wide band gap and a dense amorphous microstructure. High-temperature annealing was carried out to ensure better stoichiometry and lower absorption. Precipitation and regrowth of HfO grains were observed from 400 °C to 600 °C during annealing of the HfSiO nanocomposites, resulting in excessive surface roughness. A phenomenological model was proposed to explain the phenomenon. The HfSiO nanocomposites (x = 0.3 and 0.4) maintained a dense amorphous structure with low absorption after annealing. Finally, a 1064-nm HfSiO/SiO high-performance reflector was prepared and achieved low optical loss (15.1 ppm) and a high LIDT (67 J/cm).
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http://dx.doi.org/10.3390/ma14102606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156956PMC
May 2021

Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane.

Sci Total Environ 2021 Aug 19;780:146606. Epub 2021 Mar 19.

Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Hangzhou 310058, China. Electronic address:

Pollution induces pressure to soil microorganism; and conversely, the degradation of pollutants is reported largely regulated by the soil microbiome assembly in situ. However, the specific-dependent core taxa of degraders were barely confirmed, which is not conducive to improving the soil remediation strategy. Taking pollution of a typical organochlorine pesticide (OCP), lindane, as an example, we explored the microbial community assembly in flooded soils and simultaneously quantified the corresponding dynamics of typical soil redox processes. Contrasting initial status of microbial diversity was set up by gamma irradiation or not, with additives (acetate, NaNO acetate + NaNO) capable of modifying microbial growth employed simultaneously. Microorganism under lindane stress was reflected by microbial adaptability within complex co-occurrence networks, wherein some environment-dependent core taxa (e.g., Clostridia, Bacteroidia, Bacilli) were highly resilient to pollution and sterilization disturbances. Lindane had higher degradation rate in irradiated soil (0.96 mg kg d) than non-irradiated soil (0.83 mg kg d). In non-irradiated soil, addition of acetate promoted lindane degradation and methanogenesis, whereas nitrate inhibited lindane degradation but promoted denitrification. No significant differences in lindane degradation were observed in irradiated soils, which exhibited low-diversity microbiomes in parallel to stronger Fe reduction and methanogenesis. The varied corresponding trigger effects on soil redox processes are likely due to differences of soil microbiome, specifically, deterministic or stochastic assembly, in response to pollution stress under high or low initial microbial diversity conditions. Our results improve the knowledge of the adaptability of disturbed microbiomes and their feedback on microbial functional development in OCP-polluted soils, achieving for a more reliable understanding with respect to the ecological risk of soils resided with OCPs under the fact of global microbial diversity loss.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146606DOI Listing
August 2021
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