Publications by authors named "Bin Liu"

5,376 Publications

  • Page 1 of 1

KIF11: A potential prognostic biomarker for predicting bone metastasis-free survival of prostate cancer.

Oncol Lett 2022 Sep 15;24(3):312. Epub 2022 Jul 15.

Department of Urology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.

Most prostate cancer (PCa) cases remain indolent with a relatively good prognosis. However, bone metastasis of PCa can quickly worsen prognoses and lead to mortality. Metastasis-free survival (MFS), a strong surrogate for overall survival, is widely used in PCa prognosis research. The present study identified molecules that affect bone MFS in PCa, with clinical validation. Three datasets (GSE32269, GSE74367 and GSE77930) were downloaded from the Gene Expression Omnibus database. Hub genes most relevant to clinical traits (bone metastasis-associated morbidity) were identified by weighted gene co-expression network analysis (WGCNA) and subjected to logistic regression analysis. Patient samples were obtained between January 2014 and December 2016, with a clinically annotated follow-up in December 2021. Clinical data and follow-up information for 60 patients with PCa were used in MFS analysis. Tumor samples were retrieved, and immunohistochemistry was performed to detect vascular endothelial growth factor (VEGF). The prognostic potential of the two molecules was assessed using Cox proportional hazards regression analysis. A total of 16 gene modules were obtained via WGCNA, and the tan module, containing 147 genes, was most closely linked to bone metastasis. In total, 877 differentially expressed genes (DEGs) were detected. The DEG-tan module intersection yielded seven hub genes [BUB1, kinesin family member (KIF)2C, RACGAP1, CENPE, KIF11, TTK and KIF20A]. Using univariate and multivariate logistic regression analyses for independent risk factors of bone metastasis, KIF11 and VEGF were found to be significantly associated with a higher T stage, prostate-specific antigen level and Gleason score. In addition, KIF11 and VEGF expression levels were positively correlated (P<0.001). Using univariate Cox analysis, KIF11 and VEGF were found to exhibit a significant association with poor MFS (P<0.05). However, only KIF11 was significantly associated with MFS upon multivariate analysis (P=0.007; hazard ratio, 2.776; 95% confidence interval, 1.315-5.859). Markers of bone metastasis in PCa were identified. Overall, KIF11 is an independent indicator that can predict bone metastasis for patients with PCa, which could be used to guide clinical practice.
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http://dx.doi.org/10.3892/ol.2022.13432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353809PMC
September 2022

Synthesis of ciprofloxacin-capped gold nanoparticles conjugates with enhanced sonodynamic antimicrobial activity in vitro.

J Pharm Sci 2022 Aug 7. Epub 2022 Aug 7.

Central Hospital Affiliate to Shenyang Medical College, Shenyang 110024, China. Electronic address:

The purpose of this article is to discuss whether gold nanoparticles (GNPs) play an auxo-action on ciprofloxacin (CIP)-mediated sonodynamic antimicrobial chemotherapy (SACT) in vitro. The measuring criterion of SACT, bactericidal efficiency, was measured by plate colony-counting methods. According to research findings, the duration of ultrasound (US) exposure, solution temperature and CIP:GNPs concentration were all critical influencing factors of SACT. Furthermore, scanning electron microscopy revealed that the group of CIP:GNPs combined with US showed the most severe damaged effect on Escherichia coli and Staphylococcus aureus, resulting in the loss of their typical microbial morphology and the disclosure of contents. Therefore, the above experimental results confirmed initially that GNPs could enhance the bacteriostasis of CIP-mediated SACT. And the intracellular reactive oxygen species (ROS) detection assays proved that this acceleration might be connected to the ROS generated through the ultrasonic mechanics. In conclusion, GNPs would be regarded as a promising auxiliary material for SACT, since they are both used to be the medication carriers and sonosensitizer accelerants.
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http://dx.doi.org/10.1016/j.xphs.2022.08.004DOI Listing
August 2022

In Situ Visualization of Dynamic Cellular Effects of Phospholipid Nanoparticles via High-Speed Scanning Ion Conductance Microscopy.

Small 2022 Aug 10:e2203285. Epub 2022 Aug 10.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.

Phospholipid nanoparticles have been actively employed for numerous biomedical applications. A key factor in ensuring effective and safe applications of these nanomaterials is the regulation of their interactions with target cells, which is significantly dependent on an in-depth understanding of the nanoparticle-cell interactions. To date, most studies investigating these nano-bio interactions have been performed under static conditions and may lack crucial real-time information. It is, however, noteworthy that the nanoparticle-cell interactions are highly dynamic. Consequently, to gain a deeper insight into the cellular effects of phospholipid nanoparticles, real-time observation of cellular dynamics after nanoparticle introduction is necessary. Herein, a proof-of-concept in situ visualization of the dynamic cellular effects of sub-100 nm phospholipid nanoparticles using high-speed scanning ion conductance microscopy (HS-SICM) is reported. It is revealed that upon introduction into the cellular environment, within a short timescale of hundreds of seconds, phospholipid nanoparticles can selectively modulate the edge motility and surface roughness of healthy fibroblast and cancerous epithelial cells. Furthermore, the dynamic deformation profiles of these cells can be selectively altered in the presence of phospholipid nanoparticles. This work is anticipated to further shed light on the real-time nanoparticle-cell interactions for improved formulation of phospholipid nanoparticles for numerous bioapplications.
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http://dx.doi.org/10.1002/smll.202203285DOI Listing
August 2022

Discovery of (5-(Benzylthio)-4-(3-(trifluoromethyl)phenyl)-4-1,2,4-triazol-3-yl) Methanols as Potent Phytoene Desaturase Inhibitors through Virtual Screening and Structure Optimization.

J Agric Food Chem 2022 Aug 10. Epub 2022 Aug 10.

National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

Phytoene desaturase (PDS) is not only an important enzyme in the biosynthesis of carotenoids but also a promising target for herbicide discovery. However, in recent years, no expected PDS inhibitors with new scaffolds have been reported. Hence, a solution for developing PDS inhibitors is to search for new compounds with novel chemotypes based on the PDS protein structure. In this work, we integrated structure-based virtual screening, structure-guided optimization, and biological evaluation to discover some PDS inhibitors with novel chemotypes. It is noteworthy that the highly potent compound , 1-(4-chlorophenyl)-2-((5-(hydroxymethyl)-4-(3-(trifluoromethyl)phenyl)-4-1,2,4-triazol-3-yl)thio)ethan-1-one, exhibited a broader spectrum of post-emergence herbicidal activity at 375-750 g/ha against six kinds of weeds than the commercial PDS inhibitor diflufenican. Surface plasmon resonance (SPR) assay showed that the affinity of our compound ( = 65.9 μM) to PDS is slightly weaker but at the same level as diflufenican ( = 38.3 μM). Meanwhile, determination of the phytoene content and PDS mRNA quantification suggested that could induce PDS mRNA reduction and phytoene accumulation. Moreover, also caused the increase of reactive oxygen species (ROS) and the change of ROS-associated enzyme activity in albino leaves. Hence, all these results indicated the feasibility of PDS protein structure-based virtual screen and structure optimization to search for highly potent PDS inhibitors with novel chemotypes for weed control.
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http://dx.doi.org/10.1021/acs.jafc.2c02981DOI Listing
August 2022

Dexmedetomidine Ameliorated Cognitive Dysfunction Induced by Intestinal Ischemia Reperfusion in Mice with Possible Relation to the Anti-inflammatory Effect Through the Locus Coeruleus Norepinephrine System.

Neurochem Res 2022 Aug 9. Epub 2022 Aug 9.

Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China.

Cognitive impairment is a common central nervous system complication that occurs following surgery or organs damage outside the nervous system. Neuroinflammation plays a key role in the molecular mechanisms of cognitive impairment. Dexmedetomidine alleviates neuroinflammation and reduces cognitive dysfunction incidence; however, the mechanism by which dexmedetomidine alleviates cognitive dysfunction remains unclear. This study evaluated the effect of dexmedetomidine on attenuation of early cognitive impairment induced by intestinal ischemia-reperfusion in mice and examined whether the locus coeruleus norepinephrine (LCNE) system participates in the anti-inflammatory effect of dexmedetomidine. The superior mesenteric artery was clamped for 45 min to induce intestinal ischemia reperfusion injury. Dexmedetomidine alone or combined with DSP-4, a selective locus coeruleus noradrenergic neurotoxin, was used for pretreatment. Postoperative cognition was assessed using the Morris water maze. Serum and hippocampal levels of IL-1β, TNF-α, norepinephrine (NE), and malondialdehyde (MDA) were assessed by enzyme-linked immunosorbent assay. Immunofluorescence, immunohistochemistry, and hematoxylin and eosin staining were used to evaluate the expression of tyrosine hydroxylase (TH) in the locus coeruleus, hippocampal microglia, and intestinal injury. Pretreatment with dexmedetomidine alleviated intestinal injury and decreased the serum and hippocampal levels of NE, IL-1β, TNF-α, and MDA at 24 h after intestinal ischemia reperfusion, decreased TH-positive neurons in the locus coeruleus, and ameliorated cognitive impairment. Similarly, DSP-4 pre-treatment alleviated neuroinflammation and improved cognitive function. Furthermore, α2-adrenergic receptor antagonist atipamezole or yohimbine administration diminished the neuroprotective effects and improved cognitive function with dexmedetomidine. Therefore, dexmedetomidine attenuated early cognitive dysfunction induced by intestinal ischemia-reperfusion injury in mice, which may be related to its anti-inflammatory effects through the LCNE system.
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http://dx.doi.org/10.1007/s11064-022-03706-wDOI Listing
August 2022

Near-infrared chemiluminescent nanoprobes for deep imaging and synergistic photothermal-nitric-oxide therapy of bacterial infection.

Biomaterials 2022 Jul 31:121693. Epub 2022 Jul 31.

Institute of Molecular Aggregation Science, Tianjin University, Tianjin 300072, China. Electronic address:

Bacterial infection is the leading cause of many serious inflammation diseases threatening human health. Existing theranostic options for bacterial infection are always complicated and unsatisfactory. There is an increasing interest in developing a more effective theranostic approach for the treatment of infections. Herein, we report the development of a near-infrared (NIR) chemiluminescent (CL) nanoparticles ALPBs containing luminol, AIE dye (TTDC), PCPDTBT, and nitric oxide (NO) donor (BNN6), which could achieve a deep CL imaging-guided photothermal-NO gas therapy of bacterial infection. After intravenous injection, ALPBs could be largely accumulated in the infected site and then activated by oversecreted reactive oxygen species (ROS) to produce near-infrared chemiluminescence, which could precisely track infection-induced local inflammation. Under the guidance of imaging, synergistic photothermal-NO therapy was further performed by 808 nm laser irradiation, leading to active bacterial eradication and rapid recovery of infected tissues. The utility of ALPBs provides a powerful and controllable "all-in-one" platform for combating bacterial infection.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121693DOI Listing
July 2022

Study on the negative effect of internal-control willingness on enterprise risk-taking.

Front Psychol 2022 22;13:894087. Epub 2022 Jul 22.

School of Management, Hainan University, Haikou, China.

In the traditional cognition, the factors that affect the level of internal control are usually based on the objective factors such as corporate characteristics, financial status, and governance structure. However, the internal control defects of many famous companies expose the phenomenon of subjective manipulation, and this leads us to focus on the subjective factor of internal control, which we call internal-control willingness. We define "internal-control willingness" as the degrees of subjective initiative of the internal-control construction and execution activities. Additionally, we propose a method for measuring internal-control willingness, using text analysis and machine learning. Then, we examine the impact of internal-control willingness on enterprise risk-taking, through the internal-control, financial, and market data of China A-share main board enterprises in 2011-2018. The study found that (1) internal-control willingness has a significant positive impact on internal-control level, which can fairly achieve the measurement of internal-control subjective initiative. (2) It confirms that internal-control willingness lowers corporate risk-taking. (3) Further research finds that state-owned enterprises strengthen internal-control willingness and their risk-taking level is significantly lower than that of non-state-owned enterprises. This paper suggests that the regulatory authorities actively urge the board of directors to strengthen internal-control willingness.
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http://dx.doi.org/10.3389/fpsyg.2022.894087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355034PMC
July 2022

Rational Regulation of Electronic Structure in Layered Double Hydroxide Via Vanadium Incorporation to Trigger Highly Selective CO Photoreduction to CH.

Small 2022 Aug 7:e2202334. Epub 2022 Aug 7.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

To realize excellent selectivity of CH in CO photoreduction (CO PR) is highly desirable, yet which is challenging due to the limited active sites for CH generation and severe electron-hole recombination on photocatalysts. Herein, based on the theoretically calculated effects of vanadium incorporation into the laminate of layered double hydroxides (LDHs), V into NiAl-LDH to synthesize a series of LDHs with various V contents is introduced. NiV-LDH is revealed to afford a high CH selectivity (78.9%), and extremely low H selectivity (only 0.4%) under λ > 400 nm irradiation. By further tuning the molar ratio of Ni to V, a CH selectivity of as high as 90.1% is achieved on Ni V-LDH, and H is completely prohibited on Ni V-LDH. Fine structural characterizations and comprehensive optical and electrochemical studies uncover V incorporation creates the lower-valence Ni species as active sites for generating CH , and enhances the generation, separation, and transfer of photogenerated carriers.
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http://dx.doi.org/10.1002/smll.202202334DOI Listing
August 2022

Head-to-head comparison of Ga-PSMA-11 PET/CT and Ga-PSMA-11 PET/MRI in the detection of biochemical recurrence of prostate cancer: summary of head-to-head comparison studies.

Prostate Cancer Prostatic Dis 2022 Aug 5. Epub 2022 Aug 5.

Department of neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, China.

Purpose: Our meta-analysis aimed to evaluate the diagnostic performance of Ga-PSMA-11 PET/CT vs. Ga-PSMA-11 PET/MRI for biochemical recurrence of prostate cancer.

Methods: We searched for relevant articles in PubMed and Embase until February 2022. Studies evaluating head-to-head comparison of Ga-PSMA-11 PET/CT and Ga-PSMA-11 PET/MRI in men with prostate cancer biochemical recurrence were included. The quality of each study was assessed using the Quality Assessment of Diagnostic Performance Studies-2 (QUADAS-2) tool.

Results: A total of 5 studies with 219 patients were included in the analysis. The pooled overall detection rates of Ga-PSMA-11 PET/CT and Ga-PSMA-11 PET/MRI in detecting recurrent PCa after definitive treatment were 0.89 (95% CI: 0.65-1.00), 0.92 (95% CI: 0.77-1.00), while the detection rates were 0.20 (95% CI: 0.05-0.41) and 0.29 (95% CI: 0.10-0.53) in local recurrence, 0.51 (95% CI: 0.33-0.69) and 0.52 (95% CI: 0.44-0.61) in lymph node metastasis, 0.18 (95% CI: 0.07-0.33) and 0.20 (95% CI: 0.09-0.35) in bone metastasis. There was no significant difference between the two imaging modalities in the overall detection rate (P = 0.82). In addition, detection rates were also not significantly different in local recurrence, lymph node metastasis, or bone metastasis (P = 0.54, 1.00, 0.82).

Conclusions: Ga-PSMA-11 PET/CT and Ga-PSMA-11 PET/MRI seem to have equivalent performance in detecting biochemical recurrence in prostate cancer. However, the results of the meta-analysis were drawn from studies with small samples. Further larger studies in this setting are warranted.
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http://dx.doi.org/10.1038/s41391-022-00581-yDOI Listing
August 2022

The gut microbiome contributes to splenomegaly and tissue inflammation in a murine model of primary biliary cholangitis.

Ann Transl Med 2022 May;10(9):507

Chronic Disease Laboratory, School of Medicine, South China University of Technology, Guangzhou, China.

Background: Splenomegaly is not just a consequence of numerous chronic and acute conditions but may also contribute to their severity, due to the interaction of the spleen with the gut microbiome. This study aimed to explore the effect of the gut microbiome on splenomegaly.

Methods: We used p40IL-2Rα mice as a murine model of primary biliary cholangitis (PBC) as per our previous study. Splenomegaly was evaluated by spleen weight. Severity of liver inflammation was evaluated by hepatic mononuclear cell (MNCs) number and pathological score. Changes of immune cells in the spleen and liver were detected by flow cytometry. The effects of the gut microbiome on splenomegaly and liver inflammation were observed by combined antibiotic treatment in p40IL-2Rα mice.

Results: A proportion of p40IL-2Rα mice developed splenomegaly. The results revealed that liver mononuclear cells infiltration, histological scores of hepatic inflammation, and bile duct damage were positively correlated with the degree of splenomegaly. Hepatic CD4 and CD8 T cells numbers were significantly higher in mice with splenomegaly, and this was particularly observed in activated effector memory CD4 T and CD8 T cells. A proportion of some other immune cells including granulocytes, B, natural killer (NK), and CD8 T effector memory cells were also altered in the enlarged spleen. More importantly, administration of quadruple antibiotics to deplete gut microbiota relieved the splenomegaly of p40IL-2Rα mice, significantly alleviated liver inflammation, and caused a significant reduction of liver and spleen T cell accumulation and activation; however, single antibiotics did not induce these changes.

Conclusions: Splenomegaly was associated with more severe liver inflammation in our PBC murine model, and this effect was reversed by quadruple antibiotic treatment.
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http://dx.doi.org/10.21037/atm-21-5448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347057PMC
May 2022

Traditional Chinese Medicine in Treating Children With Coronavirus Disease 2019: A Scoping Review.

Front Pediatr 2022 19;10:935551. Epub 2022 Jul 19.

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Science, Beijing, China.

Coronavirus disease 2019 (COVID-19) is currently widely spread across the world. Traditional Chinese Medicine (TCM) plays an important role in the overall treatment process. As a special group of population, the treatment outcome of children with COVID-19 has attracted much attention. Our study summarizes the current situation of TCM treatment of children with COVID-19. The results showed that TCM displayed a positive role in the treatment process, and that no significant adverse reactions were found. Our findings provide analytical evidence for the efficacy and safety of TCM participation in the treatment of COVID-19 in children.
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http://dx.doi.org/10.3389/fped.2022.935551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343629PMC
July 2022

Plasma exosomal microRNAs Associated with Metabolism as Early Predictor of Gestational Diabetes Mellitus.

Diabetes 2022 Aug 4. Epub 2022 Aug 4.

Department of Obstetrics and Gynecology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China.

To date, the microRNA (miRNA) expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small RNA sequencing in 12 GDM and 12 normal glucose tolerance (NGT) pregnant women and validated in 102 GDM and 101 NGT pregnant women. A total of 22 exosomal miRNAs were found and five of them were verified by qRT-PCR. Exosmal miR-423-5p was upregulated, while miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy is complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p, were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs showed an AUC of 0.82 (95%CI, 0.73∼0.91) in early prediction of GDM. Our study demonstrated that dysregulated exosomal miRNAs in plasma from GDM pregnant women might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM.
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http://dx.doi.org/10.2337/db21-0909DOI Listing
August 2022

Efficacy and safety of filgotinib in patients with rheumatoid arthritis and inadequate response to disease-modifying antirheumatic drugs (DMARDs): A meta-analysis of randomized controlled trials.

ARP Rheumatol 2022 Apr 28. Epub 2022 Apr 28.

the First Hospital of Jilin University.

Background: Filgotinib has been approved for the treatment of rheumatoid arthritis (RA) in adults who respond inadequately to disease-modifying antirheumatic drugs (DMARDs) in Europe and Japan. Several randomized controlled trials (RCTs) have investigated its efficacy and safety in adult patients with RA. This meta-analysis aimed to study the efficacy and safety of filgotinib in patients with RA withan inadequate response to methotrexateor other DMARDs.

Methods: A systematic literature search was conducted to identify articles in PubMed, MEDLINE, EMBASE, and Cochrane Library from inceptionto December 1, 2021. Outcomes of interest included ACR20/50/70 responses, DAS28-CRP ≤ 3.2, SF-36 PCS Score, FACIT-fatigue, SDAI,CDAI, and HAQ-DI, which were assessed after treatment. The safety outcomes included treatment-emergent adverse events (TEAEs) and serious TEAEs. Odds ratios (ORs) with 95% confidence intervals (CI) were pooled for categorical variables, and the mean difference with 95%CI were pooled for continuous variables. We used Review Manager 5.3 for the standard meta-analysis. This study followed the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA).

Results: Four RCTs comparing filgotinib (200 and 100 mg once daily) with placebo were identified. Compared with placebo, 200 and 100 mg filgotinib was more effective in achieving ACR20/50/70 responses and other outcomes at weeks 12 and 24 (P < 0.05), with no significant difference in safety outcomes (P > 0.05). Filgotinib 200 mg performed better than filgotinib 100 mg in terms of ACR20/50 responses, DAS28-CRP ≤ 3.2, SDAI, and CDAI at weeks 12 and 24, and caused fewer serious TEAEs than the 100 mg dose.

Conclusions: Filgotinib is effective in the treatment of RA, and the 200 mg dose has a more beneficialprofile thanthe 100 mg dose.
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April 2022

Pulsed hydraulic-pressure-responsive self-cleaning membrane.

Nature 2022 08 3;608(7921):69-73. Epub 2022 Aug 3.

State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing, China.

Pressure-driven membranes is a widely used separation technology in a range of industries, such as water purification, bioprocessing, food processing and chemical production. Despite their numerous advantages, such as modular design and minimal footprint, inevitable membrane fouling is the key challenge in most practical applications. Fouling limits membrane performance by reducing permeate flux or increasing pressure requirements, which results in higher energetic operation and maintenance costs. Here we report a hydraulic-pressure-responsive membrane (PiezoMem) to transform pressure pulses into electroactive responses for in situ self-cleaning. A transient hydraulic pressure fluctuation across the membrane results in generation of current pulses and rapid voltage oscillations (peak, +5.0/-3.2 V) capable of foulant degradation and repulsion without the need for supplementary chemical cleaning agents, secondary waste disposal or further external stimuli. PiezoMem showed broad-spectrum antifouling action towards a range of membrane foulants, including organic molecules, oil droplets, proteins, bacteria and inorganic colloids, through reactive oxygen species (ROS) production and dielectrophoretic repulsion.
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http://dx.doi.org/10.1038/s41586-022-04942-4DOI Listing
August 2022

Repeated Sevoflurane Exposures in Neonatal Rats Increased the Brain Vulnerability to Future Stress Exposure and Resulted in Fear Extinction Deficit.

Neurotox Res 2022 Aug 2. Epub 2022 Aug 2.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.

Sevoflurane anesthesia during neonatal period was reported to sensitize the rodent animals to stress later in life. The authors tested the hypothesis that repeated sevoflurane exposures in neonatal rats increased the brain vulnerability to future stress exposure and resulted in fear extinction deficit and investigated whether the neonatal brain depolarizing γ-aminobutyric acid type A receptor (GABAR) is involved in mediating these abnormalities. Neonatal Sprague-Dawley male rats, pretreated with vehicle or the NKCC1 inhibitor, bumetanide, received sequential exposures to 3% sevoflurane for 2 h on postnatal days (P) 5, P6, and P7 and then were exposed to electric foot shock stress in fear conditioning training at P14. Juvenile rats at different developmental brain stage receiving identical sevoflurane exposures on P25, P26, and P27 were also studied. The results showed repeated sevoflurane exposures in neonatal rats and increased the cation-chloride cotransporters NKCC1/KCC2 ratio in the PFC at P14. Repeated exposures to sevoflurane in neonatal rather than juvenile rats enhanced the stress response and exacerbated neuroapoptosis in the PFC after exposed to electric foot shock in fear conditioning training. Neonatal rather than juvenile sevoflurane-exposed rats exhibited deficits in fear extinction training and recall. Pretreatment of neonatal rats prior to sevoflurane exposures with bumetanide reduced the NKCC1/KCC2 ratio at P14 and ameliorated most of the subsequent adverse effects. Our study indicates that repeated sevoflurane exposures in neonatal rats might increase the brain vulnerability to future stress exposure and resulted in fear extinction deficit, which might be associated with the neonatal enhanced brain depolarizing GABAR activity.
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http://dx.doi.org/10.1007/s12640-022-00529-zDOI Listing
August 2022

Improving the Intrinsic Stretchability of Fully Conjugated Polymer for Deep-Blue Polymer Light-Emitting Diodes with a Narrow Band Emission: Benefits of Self-Toughness Effect.

J Phys Chem Lett 2022 Aug 2;13(31):7286-7295. Epub 2022 Aug 2.

Key Laboratory of Flexible Electronics (KLoFE) and Institute of Advanced Materials (IAM), Nanjing Tech University (Nanjing Tech), 30 South Puzhu Road, Nanjing 211816, China.

It is challenging to construct the intrinsically stretchable active layer of rigid conjugated polymers (CPs) toward flexible deep-blue light-emitting diodes (FLEDs). Inspired by the self-toughness effect, sacrificial hydrogen bonding (H-bonding) and a cross-linked network synergistically enabled polydiarylfluorene (PFs-NH) films to present efficient deep-blue emission and excellent intrinsic stretchability. In particular, a cross-linked network structure presenting viscoelasticity behaviors, which was successfully inherited into postprocessed films with interchain interpenetration and a crystallinity domain and behaved as energy absorption and dissipation centers, was induced by the interchain H-bonding interaction in toluene (Tol) precursor solutions where the storage moduli (') gradually exceeded the loss moduli (″). Subsequently, intrinsic stretchable films with a tensile rate of 30% were prepared from Tol solutions, different from the brittle films from polar solvents. Eventually, narrow band, deep-blue PLEDs showed a maximum EQE of 1.28% and a full width half-maximum (fwhm) of 28 nm. Therefore, the self-toughness effect induced by hierarchical structures will be feasible to obtain high-performance FLEDs.
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http://dx.doi.org/10.1021/acs.jpclett.2c02071DOI Listing
August 2022

Stable solar water splitting with wettable organic-layer-protected silicon photocathodes.

Nat Commun 2022 Aug 1;13(1):4460. Epub 2022 Aug 1.

School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Protective layers are essential for Si-based photocathodes to achieve long-term stability. The conventionally used inorganic protective layers, such as TiO, need to be free of pinholes to isolate Si from corrosive solution, which demands extremely high-quality deposition techniques. On the other hand, organic hydrophobic protective layers suffer from the trade-off between current density and stability. This paper describes the design and fabrication of a discontinuous hybrid organic protective layer with controllable surface wettability. The underlying hydrophobic layer induces the formation of thin gas layers at the discontinuous pores to isolate the electrolyte from Si substrate, while allowing Pt co-catalyst to contact the electrolyte for water splitting. Meanwhile, the surface of this organic layer is modified with hydrophilic hydroxyl groups to facilitate bubble detachment. The optimized photocathode achieves a stable photocurrent of 35 mA/cm for over 110 h with no trend of decay.
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http://dx.doi.org/10.1038/s41467-022-32099-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343433PMC
August 2022

Analysis of Strategies and Skills of English Translation Based on Coverage Mechanism.

Authors:
Bin Liu Jing Wang

Comput Intell Neurosci 2022 21;2022:7767045. Epub 2022 Jul 21.

School of Foreign Studies, Suqian University, Jiangsu, Suqian 223800, China.

In order to alleviate the problem of over translation and missing translation in NMT, based on the consistency and complementarity of information stored in different covering models, a multicoverage fusion model is proposed, which uses coverage vector and coverage score to guide the attention mechanism at the same time. First, the concept level definitions of words are covered. Then, two kinds of translation history information stored in the cover vector and cover score are used to guide the calculation of the attention score at the same time. Finally, the dual attention decoding method based on the fusion coverage mechanism is adopted. The experimental results show that the multicoverage fusion model can improve the translation quality of NMT.
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http://dx.doi.org/10.1155/2022/7767045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334112PMC
August 2022

Obstructive sleep apnea and serum total testosterone: a system review and meta-analysis.

Sleep Breath 2022 Jul 29. Epub 2022 Jul 29.

Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou, China.

Purpose: Testosterone deficiency (TD) negatively affects male sexuality, reproduction, general health, and quality of life. In recent years, decreased serum testosterone levels have been reported to be caused by obstructive sleep apnea (OSA). However, these results are controversial and lack the support of a large number of high-quality studies. Hence, we performed a meta-analysis to assess the association between OSA and serum testosterone levels.

Methods: To identify eligible studies, we conducted a systematic retrieval in the electronic databases (PubMed, Web of Science, the Cochrane Library, EMBASE) from their inception to September 2021. We chose studies with definitive diagnoses of OSA, including effects of OSA on testosterone level. Random effect model was used for analysis.

Results: This meta-analysis included 24 case-control studies with 1389 patients (1268 male patients) and 845 controls (745 male control). The serum testosterone levels in the male OSA group were significantly lower than that of control group [SMD = - 0.97, 95% CI (- 1.47, - 0.47)], while there was no difference in female patients with OSA and control [SMD = 0.06, 95% CI (- 0.22, 0.33)]. Subgroup analysis showed that race, age, body mass index (BMI), and detection method were the reason for high heterogeneity (I = 94.9%).

Conclusions: The results indicated that OSA is significantly correlated with the decrease in serum testosterone levels in men. Male patients with OSA should be alerted to secondary diseases caused by low testosterone levels.
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http://dx.doi.org/10.1007/s11325-022-02655-6DOI Listing
July 2022

Digital design and evaluation for additive manufacturing of personalized myopic glasses.

Sci Rep 2022 Jul 28;12(1):12926. Epub 2022 Jul 28.

Fujian Provincial Key Laboratory of Special Energy Manufacturing, Xiamen Key Laboratory of Digital Vision Measurement, Huaqiao University, Xiamen, 361021, China.

Myopic glasses design has critical effects on the match between glasses and individual face. Improper myopic glasses design may affect the try-on comfort and health. It is difficult for the myopic glasses to be adjusted variedly and suitably from people to people with the limitations of traditional manufacturing processes and current design methods. In this paper, an evaluation descriptor named glasses fit score, which contains alignment scores and design scores, is proposed to guide and evaluate the myopic glasses design. Based on the descriptor, a novel approach is presented to complete the myopic glasses design and manufacturing individually. The approach can be divided into three steps: glasses alignment, glasses personalized design and glasses manufacturing. During the glasses alignment, the myopic glasses are aligned to the face to obtain the alignment score of the descriptor based on the face symmetry plane and feature points, including the silent point of the eye and the top point of the ear. After the glasses alignment, the myopic glasses can be deformed to match the face to achieve the design score of the descriptor. The deformations include glasses frame transformation, glasses leg option and glasses personalized mark. In the glasses manufacturing, the designed myopic glasses can be fabricated by a 3D printer. Then, a post processing process is conducted to polish the myopic glasses with oil painted. Finally, the proposed approach is applied to adjust the myopic glasses for several people. The results show that, compared to the previous methods, the approach can make the myopic glasses aligned and deformed to the individual face effectively to obtain an ideal score of the descriptor, thus improving the match between the glasses and the individual face.
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http://dx.doi.org/10.1038/s41598-022-17233-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334356PMC
July 2022

Analysis of Pulmonary Function in Thymoma Subjects: A 20-Year Retrospective Cohort Study.

Thorac Cardiovasc Surg 2022 Jul 27. Epub 2022 Jul 27.

Beijing Chest Hospital, Beijing, China.

Background:  Thymoma is the most common tumor of the anterior mediastinum. However, the correlation between thymoma stage and pulmonary function was not assessed. Our objective in this study was to describe the pulmonary function in thymoma subjects stratified with different staging systems.

Methods:  A total of 143 subjects with a diagnosis of thymoma who underwent extended thymectomy for thymoma between January 2001 and December 2019 were reviewed retrospectively. All the subjects experienced pulmonary function tests (PFTs) using Master Screen PFT system and total respiratory resistance measurement.

Results:  We evaluated 143 subjects with a diagnosis of thymoma; the significant differences were observed in mean values of vital capacity, inspiratory volume (IC), total lung capacity (TLC), ratio of residual volume to total lung capacity (RV/TLC), forced vital capacity, forced expiratory volume in 1 second, ratio of forced expiratory volume in 1 second to forced vital capacity, peak expiratory flow, peak inspiratory flow, maximum ventilation volume, total airway resistance, and diffusing capacity for carbon monoxide (DLCO) across upper airway obstruction classification. PFTs of subjects with varying Masaoka stages are different. RV and RV/TLC of subjects in stages III and IV were higher than those of normal level, while DLCO of subjects in stage IV was lower than the normal level, and the mean level of IC showed significant difference between stage II and stage III.

Discussion:  The pulmonary function patterns of thymoma subjects significantly correlate with tumor location and size rather than clinical Masaoka stage.
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http://dx.doi.org/10.1055/s-0042-1749320DOI Listing
July 2022

Efficacy of Triple and Talonavicular Arthrodesis for the Treatment of III-V Müller-Weiss Disease.

Tohoku J Exp Med 2022 Jul 28. Epub 2022 Jul 28.

Department of Orthopedic Surgery, Tongji Hospital of Tongji University.

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http://dx.doi.org/10.1620/tjem.2022.J062DOI Listing
July 2022

Calibration of Radar RCS Measurement Errors by Observing the Luneburg Lens Onboard the LEO Satellite.

Sensors (Basel) 2022 Jul 20;22(14). Epub 2022 Jul 20.

State Key Laboratory of Astronautic Dynamics, Xi'an Satellite Control Center, Xi'an 710043, China.

Accurate radar RCS measurements are critical to the feature recognition of spatial targets. A calibration method for radar RCS measurement errors is proposed for the first time in the context of special target tracking by observing the Luneburg Lens onboard the LEO satellite. The Luneburg Lens has favorable RCS scattering properties for the radar microwave. Thus, the laboratory RCS measurements of the Luneburg Lens, with some fixed incident frequency and with different incident orientations for the radar microwave, will be implemented in order to build a database. The incident orientation for the radar microwave in the satellite body frame will be calculated by taking advantage of the precise orbit parameters, with errors only at the magnitude of several centimeters and within the actual satellite attitude parameters. According to the incident orientation, the referenced RCS measurements can be effectively obtained by the bilinear interpolation in the database. The errors of actual RCS measurements can thus be calibrated by comparing the referenced and the actual RCS measurements. In the RCS measurement experiment, which lasts less than 400 s, the actual RCS measurement errors of the Luneburg Lens are nearly less than 0 dBsm, which indicates that the RCS measurement errors of the spatial targets can be effectively calculated by the proposed calibration method. After the elaborated calibration, the RCS measurements of the spatial targets can be accurately obtained by radar tracking.
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http://dx.doi.org/10.3390/s22145421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325169PMC
July 2022

Fabrication of Tissue-Engineered Cartilage Using Decellularized Scaffolds and Chondrocytes.

Polymers (Basel) 2022 Jul 13;14(14). Epub 2022 Jul 13.

Institute of Orthopaedics, 301 Hospital of the Chinese People's Liberation Army General Hospital, Beijing 100120, China.

In this paper, we aim to explore the application value of tissue engineering for the construction of artificial cartilage in vitro. Chondrocytes from healthy porcine articular cartilage tissue were seeded on articular cartilage extracellular matrix (ACECM) scaffolds and cultivated. Type II collagen immunofluorescent staining was used to assess secretion from the extracellular matrix. Chondrocytes, which were mainly polygonal and cobblestone-shaped, were inoculated on ACECM-oriented scaffolding for 7 days, and the neo-tissue showed translucent shape and toughness. Using inverted and fluorescence microscopy, we found that chondrocytes on the scaffolds performed well in terms of adhesion and growth, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds had good biocompatibility. The inflammatory cell detection, cellular immune response assay and humoral immune response assay showed porcine ACECM scaffolds were used for xenotransplantation without significant immune inflammatory response. All these findings reveal that ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
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http://dx.doi.org/10.3390/polym14142848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316171PMC
July 2022

AlGaN Quantum Disk Nanorods with Efficient UV-B Emission Grown on Si(111) Using Molecular Beam Epitaxy.

Nanomaterials (Basel) 2022 Jul 21;12(14). Epub 2022 Jul 21.

Key Laboratory of Advanced Photonic and Electronic Materials, School of Electronic Science and Engineering, Nanjing University, Nanjing 210023, China.

AlGaN nanorods have attracted increasing amounts of attention for use in ultraviolet (UV) optoelectronic devices. Here, self-assembled AlGaN nanorods with embedding quantum disks (Qdisks) were grown on Si(111) using plasma-assisted molecular beam epitaxy (PA-MBE). The morphology and quantum construction of the nanorods were investigated and well-oriented and nearly defect-free nanorods were shown to have a high density of about 2 × 10 cm. By controlling the substrate temperature and Al/Ga ratio, the emission wavelengths of the nanorods could be adjusted from 276 nm to 330 nm. By optimizing the structures and growth parameters of the Qdisks, a high internal quantum efficiency (IQE) of the AlGaN Qdisk nanorods of up to 77% was obtained at 305 nm, which also exhibited a shift in the small emission wavelength peak with respect to the increasing temperatures during the PL measurements.
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http://dx.doi.org/10.3390/nano12142508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319290PMC
July 2022

Evaluation on Temperature-Dependent Transient Instability in p-GaN Gate HEMTs under Negative Gate Stress by Fast Sweeping Characterization.

Micromachines (Basel) 2022 Jul 12;13(7). Epub 2022 Jul 12.

Jiangsu Provincial Key Laboratory of Advanced Photonic and Electronic Materials, School of Electronic Science and Engineering, Nanjing University, Nanjing 210093, China.

In this work, temperature-dependent transient threshold voltage () instability behaviors in p-GaN/AlGaN/GaN HEMTs, with both Schottky gate (SG) and Ohmic gate (OG), were investigated systematically, under negative gate bias stress, by a fast voltage sweeping method. For SG devices, a concave-shaped evolution gradually occurs with the increase in temperature, and the concave peak appears faster with increasing reverse bias stress, followed by a corresponding convex-shaped recovery process. In contrast, the concave-shaped evolution for OG devices that occurred at room temperature gradually disappears in the opposite shifting direction with the increasing temperature, but the corresponding convex-shaped recovery process is not observed, substituted, instead, with a quick and monotonic recovery process to the initial state. To explain these interesting and different phenomena, we proposed physical mechanisms of time and temperature-dependent hole trapping, releasing, and transport, in terms of the discrepancies in barrier height and space charge region, at the metal/p-GaN junction between SG and OG HEMTs.
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http://dx.doi.org/10.3390/mi13071096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319980PMC
July 2022

A Novel NDT Scanning System Based on Line Array Fast Neutron Detector and D-T Neutron Source.

Materials (Basel) 2022 Jul 15;15(14). Epub 2022 Jul 15.

Institute of Nuclear Physics and Chemistry, Mianyang 621900, China.

A novel non-destructive testing scanning system based on a large-size line array fast neutron detector and compact D-T neutron source has been constructed. The scanning range is up to 1000 mm, and the resolution is better than 1 mm. The fast neutron detection subsystem consists of a polypropylene zinc sulfide scintillator embedded with wavelength-shifting fibers, coupled with a light lens and a scientific CCD camera. With a new rotating tritium target, the lifetime of the compact D-T neutron source could achieve ten hours. The experimental results indicate that the scanning method based on line array fast neutron detector and D-T neutron source is feasible and enables the detection of slits on the order of 0.5 mm in width. Fast neutron tomography has been realized by this detection system too.
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http://dx.doi.org/10.3390/ma15144946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319683PMC
July 2022

Investigation of a Novel Hydrogen Depressurization Structure Constituted by an Orifice Plate with Tesla-Type Channels.

Materials (Basel) 2022 Jul 14;15(14). Epub 2022 Jul 14.

School of Mechanical, Materials, Mechatronic and Biomedical Engineering, University of Wollongong, Wollongong, NSW 2522, Australia.

A hydrogen depressurization system is required to supply the hydrogen to the fuel cell stack from the storage. In this study, a Tesla-type depressurization construction is proposed. Parallel Tesla-type channels are integrated with the traditional orifice plate structure. A computational fluid dynamics (CFD) model is applied to simulate high-pressure hydrogen flow through the proposed structure, using a commercial software package, ANSYS-Fluent (version 19.2, ANSYS, Inc. Southpointe, Canonsburg, PA, USA). The Peng-Robinson (PR) equation of state (EoS) is incorporated into the CFD model to provide an accurate thermophysical property estimation. The construction is optimized by the parametric analysis. The results show that the pressure reduction performance is improved greatly without a significant increase in size. The flow impeding effect of the Tesla-type orifice structure is primarily responsible for the pressure reduction improvement. To enhance the flow impeding effect, modifications are introduced to the Tesla-type channel and the pressure reduction performance has been further improved. Compared to a standard orifice plate, the Tesla-type orifice structure can improve the pressure reduction by 237%. Under low inlet mass flow rates, introduction of a secondary Tesla-type orifice construction can achieve better performance of pressure reduction. Additionally, increasing parallel Tesla-type channels can effectively reduce the maximum Mach number. To further improve the pressure reduction performance, a second set of Tesla-type channels can be introduced to form a two-stage Tesla-type orifice structure. The study provides a feasible structure design to achieve high-efficiency hydrogen depressurization in hydrogen fuel cell vehicles (HFCVs).
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http://dx.doi.org/10.3390/ma15144918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320887PMC
July 2022

Metabolomics Analysis Coupled with Weighted Gene Co-Expression Network Analysis Unravels the Associations of Tricarboxylic Acid Cycle-Intermediates with Edible Pigments Produced by (Hong Qu).

Foods 2022 Jul 21;11(14). Epub 2022 Jul 21.

TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China.

azaphilones pigments (MonAzPs) produced by microbial fermentation are widely used as food chemicals for coloring and supplying beneficial biological attributes. In this study, a fermentation perturbation strategy was implemented by separately adding different amino acids, and detecting the intracellular metabolome via UHPLC-Q-Orbitrap HRMS. With the aid of weighted gene co-expression network analysis, two metabolic intermediates, fumarate and malate, involved in the tricarboxylic acid cycle, were identified as the hub metabolites. Moreover, exogenous addition of fumarate or malate significantly promoted red pigment production, and reduced orange/yellow pigment production. The importance of the tricarboxylic acid cycle was further emphasized by detecting intracellular levels of ATP, NAD(P)H, and expression of oxidoreductase-coding genes located in the MonAzPs synthetic gene cluster, suggesting a considerable effect of the energy supply on MonAzPs synthesis. Collectively, metabolomics is a powerful approach to position the crucial metabolic regulatory factors, and facilitate the development of engineering strategies for targeted regulation, lower trial-and-error cost, and advance safe and controllable processes for fermented food chemistry industries.
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http://dx.doi.org/10.3390/foods11142168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320606PMC
July 2022

Correlation between plasma sphingosine-1-phosphate and the occurrence and severity of coronary heart disease in postmenopausal women.

Menopause 2022 Aug;29(8):920-925

From the Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Objective: Sphingosine-1-phosphate (S1P) is a bioactive sphingosine with antiatherosclerotic effects. The incidence of coronary heart disease (CHD) increases significantly among women after menopause. We explored the relationship between plasma S1P levels and the occurrence and severity of CHD in postmenopausal women.

Methods: Postmenopausal women admitted to our hospital for coronary angiography because of chest pain-like symptoms were included in our study. By 1:1 age matching (age difference ≤5 y), 166 women in the CHD group and control group were enrolled. The plasma S1P concentration was determined, and the Gensini score was calculated to decide the severity of CHD.

Results: Plasma S1P levels were significantly lower in the CHD group of postmenopausal women ( P < 0.001). S1P (odds ratio, 0.952; 95% CI, 0.934-0.970) was an independent predictor of the occurrence of CHD in postmenopausal women. The area under the curve for S1P to predict the occurrence of CHD was 0.653 (95% CI, 0.595-0.712), and the cutoff value was 96.89 ng/mL. The plasma S1P level was the lowest in the high-tertile group of the Gensini score ( P < 0.001), and the plasma S1P (odds ratio, 0.948; 95% CI, 0.926-0.970) was an independent predictor of a high Gensini score in postmenopausal women with CHD.

Conclusions: Plasma S1P is an independent risk factor of the occurrence and severity of CHD in postmenopausal women. The occurrence and aggravation of CHD in postmenopausal women may be related to levels of S1P.
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http://dx.doi.org/10.1097/GME.0000000000002004DOI Listing
August 2022
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