Publications by authors named "Bin Jin"

78 Publications

A Bulk-Heterostructure Nanocomposite Electrolyte of CeSmO-SrTiO for Low-Temperature Solid Oxide Fuel Cells.

Nanomicro Lett 2021 Jan 4;13(1):46. Epub 2021 Jan 4.

School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Republic of Singapore.

Since colossal ionic conductivity was detected in the planar heterostructures consisting of fluorite and perovskite, heterostructures have drawn great research interest as potential electrolytes for solid oxide fuel cells (SOFCs). However, so far, the practical uses of such promising material have failed to materialize in SOFCs due to the short circuit risk caused by SrTiO. In this study, a series of fluorite/perovskite heterostructures made of Sm-doped CeO and SrTiO (SDC-STO) are developed in a new bulk-heterostructure form and evaluated as electrolytes. The prepared cells exhibit a peak power density of 892 mW cm along with open circuit voltage of 1.1 V at 550 °C for the optimal composition of 4SDC-6STO. Further electrical studies reveal a high ionic conductivity of 0.05-0.14 S cm at 450-550 °C, which shows remarkable enhancement compared to that of simplex SDC. Via AC impedance analysis, it has been shown that the small grain-boundary and electrode polarization resistances play the major roles in resulting in the superior performance. Furthermore, a Schottky junction effect is proposed by considering the work functions and electronic affinities to interpret the avoidance of short circuit in the SDC-STO cell. Our findings thus indicate a new insight to design electrolytes for low-temperature SOFCs.
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http://dx.doi.org/10.1007/s40820-020-00574-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187505PMC
January 2021

The prognostic value of sarcopenia combined with preoperative fibrinogen-albumin ratio in patients with intrahepatic cholangiocarcinoma after surgery: A multicenter, prospective study.

Cancer Med 2021 Jul 8;10(14):4768-4780. Epub 2021 Jun 8.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: To explore the prognostic value of the fibrinogen-albumin ratio (FAR) combined with sarcopenia in intrahepatic cholangiocarcinoma (ICC) patients after surgery and to develop a nomogram for predicting the survival of ICC patients.

Materials And Methods: In this prospective cohort study, 116 ICC patients who underwent radical surgery were enrolled as the discovery cohort and another independent cohort of 68 ICC patients was used as the validation cohort. Kaplan-Meier method was used to analyze prognosis. The independent predictor of overall survival (OS) and recurrence-free survival (RFS) was evaluated by univariable and multivariable Cox regression analyses, then developing nomograms. The performance of nomograms was evaluated by concordance index (C-index), calibration curve, receiver operating characteristic curve analysis (ROC), and decision curve analysis (DCA).

Results: Patients with high FAR had lower OS and RFS. FAR and sarcopenia were effective predictors of OS and RFS. Patients with high FAR and sarcopenia had a poorer prognosis than other patients. OS nomogram was constructed based on age, FAR, and sarcopenia. RFS nomogram was constructed based on FAR and sarcopenia. C-index for the nomograms of OS and RFS was 0.713 and 0.686. Calibration curves revealed great consistency between actual survival and nomogram prediction. The area under ROC curve (AUC) for the nomograms of OS and RFS was 0.796 and 0.791 in the discovery cohort, 0.823 and 0.726 in the validation cohort. The clinical value of nomograms was confirmed by the DCA.

Conclusions: ICC patients with high FAR and sarcopenia had a poor prognosis, the nomograms developed based on these two factors were accurate and clinically useful in ICC patients who underwent radical resection.
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http://dx.doi.org/10.1002/cam4.4035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290250PMC
July 2021

Liver retraction using n-butyl-2-cyanoacrylate glue during laparoscopic cholecystectomy.

Medicine (Baltimore) 2021 May;100(20):e25879

Department of General Surgery, Qilu Hospital of Shan Dong University, Jinan, China.

Abstract: There might be a thick "protrusion" in the visceral surface of hepatic quadrate lobe during the laparoscopic cholecystectomy (LC), which affects the surgical fields and consequently triggers high risks of biliary tract injury. Although n-butyl-2-cyanoacrylate (NBCA) glue has been applied to laparoscopic upper abdominal surgery for liver retraction, there is still no consensus on its safety and feasibility in LC. In this study, we investigated the safety, feasibility, and effectiveness of liver retraction using NBCA glue for these patients which have the thick "protrusion" on the square leaf surface of the liver during LC.Fifty-seven patients presenting thick "protrusion" hepatic quadrate lobe were included in our retrospective study. We performed LC in the presence of NBCA glue (n = 30, NBCA group) and absence of NBCA glue (n = 27, non-NBCA group), respectively. NBCA was used to fix the thick "protrusion" of the liver leaves to the hepatic viscera surface, which contributed to the revelation of the gallbladder triangle. The operation time, blood loss, postoperative hospitalization, and liver function were compared between the 2 groups.Both the groups' patients accomplished the operation in the laparoscopy. There was no mortality and no additional incision during operation. No severe complications including bile duct injury were available after surgery and no postoperative NBCA-related complications occurred after 9- to 30 months' follow-up. The time of operation in NBCA group showed significant decrease compared with that of non-NBCA group (48.33 ± 16.15 vs 65.00 ± 22.15 minutes, P < .01). There were no significant differences in blood loss, postoperative hospital stays, and the preoperative and postoperative liver function between the two groups (P > .05). Besides, no significant differences were noticed in major clinical characteristics between the 2 groups (P > .05).Liver retraction using NBCA during LC for thick "protrusion" hepatic quadrate lobe patients is safe, effective, and feasible.
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http://dx.doi.org/10.1097/MD.0000000000025879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137017PMC
May 2021

Antimony tin oxide/lead selenide composite as efficient counter electrode material for quantum dot-sensitized solar cells.

J Colloid Interface Sci 2021 Sep 17;598:492-499. Epub 2021 Apr 17.

Key Laboratory of Macromolecular Science of Shaanxi Province, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address:

Antimony tin oxide (ATO)/lead selenide (PbSe) composite was rationally designed and fabricated on fluorine doped tin oxide glass (FTO) for using as counter electrode (CE) of quantum dot sensitized solar cells (QDSSCs). The electrocatalytic activity of the CE is deeply investigated in the polysulfide electrolyte by employing the Tafel, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) of the symmetrical cells. The results confirm that the ATO/PbSe CE has better electrocatalytic activity and stability than that of PbSe CE obtained by pulse voltage electrodeposition (PVD). The enhanced electrocatalytic performance of ATO/PbSe CE can be attributed to its high specific surface area, excellent permeability, conductivity and interface connectivity, which provide more electrocatalytic active sites for the reduction of polysulfide species, as well as fast channels for ions diffusion and electron transport. As a result, the CdS QDSSCs and CdS/CdSe co-sensitized QDSSCs assembled by the ATO/PbSe CE exhibits better power conversion efficiency (η) of 1.72% and 5.59%, respectively than that of PbSe CE obtained by PVD. Furthermore, photovoltaic property of the ATO/PbSe CE in CdS/CdSe co-sensitized QDSSCs keeps stable for over 200 min. This present work provides a simple and effective strategy for the construction of high-performance CE materials of QDSSCs.
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http://dx.doi.org/10.1016/j.jcis.2021.04.073DOI Listing
September 2021

Clinical Application of a Modified Double Purse-String Continuous Suture Technique for Pancreaticojejunostomy: Reliable for Laparoscopic Surgery and Small Size Main Pancreatic Duct.

Biomed Res Int 2021 13;2021:6676999. Epub 2021 Mar 13.

Department of General Surgery, Qilu Hospital, Shandong University, No. 107, Wenhua Xi Road, Jinan 250012, China.

Background: The technical challenge of pancreatojejunostomy (PJ) is the greatest barrier for surgeons to complete pancreatoduodenectomy (PD). The authors present an easy-to-master PJ anastomosis technique with limited technical requirements. This technique uses two layers of sutures and double purse-string sutures to complete the entire anastomosis. This anastomosis technique has achieved good results in laparoscopic surgery (LS) and small size main pancreatic duct (MPD).

Methods: From February 2015 to August 2020, 63 patients who met the surgical indications underwent a modified double purse-string continuous suture pancreaticojejunostomy technique in our center. We collected patient demographic characteristics and perioperative outcomes and analyzed these data.

Results: A total of 63 patients underwent PD using our new anastomosis technique. Thirty-eight patients underwent LS, and 26 patients had a small MPD (<3 mm). The median operative time (OT) was 270 min, and the median estimated blood loss (EBL) was 200 ml. Ten patients had grade B postoperative pancreatic fistula (POPF), while no patients had grade C POPF. No 90-day mortality was observed. There were significant differences in the OT and postoperative hospital stay (PHS) among groups with different surgical procedures, while there were no significant differences among groups with different MPD sizes. Neither the surgical procedure nor the MPD size affected early postoperative complications.

Conclusion: This new technique can not only reduce the incidence of POPF but also is reliable for LS and surgeries with small size MPD. Therefore, this technique is worthy of clinical promotion and application in the future.
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http://dx.doi.org/10.1155/2021/6676999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984902PMC
May 2021

Oxytocin promotes hepatic regeneration in elderly mice.

iScience 2021 Feb 2;24(2):102125. Epub 2021 Feb 2.

Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.

Liver aging impairs the ability of hepatocyte regeneration. Recent studies have found that oxytocin (OT) plays an important role in promoting tissue repair and maintaining differentiation and regeneration of stem cells. Here, we reported that OT receptors, which are specifically located in hepatocytes, decrease with aging in human and mice. Interestingly, the level of serum OT also decline with age. Notably, OT promotes hepatocyte regeneration only in aged mice but not in young mice and . Further studies reveal that OT promotes autophagy in either AML12 mouse hepatocytes or aged mice after partial hepatectomy or with CCl4-induced acute liver injury. In conclusion, OT promotes liver regeneration, especially in aged mice, which may be achieved by promoting autophagy. All these results support the possibility of OT and its analog being a potent anti-aging drug and promote liver rejuvenation.
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http://dx.doi.org/10.1016/j.isci.2021.102125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895748PMC
February 2021

Effects of (Dunn) Nakai on the osteogenic differentiation of stem cells.

Mol Med Rep 2021 04 12;23(4). Epub 2021 Feb 12.

Department of Biosystems Engineering, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.

(Dunn) Nakai, commonly known as gondre, is a perennial herb that grows predominantly in South Korea. It contains several bioactive phytochemicals with antioxidant, anti‑cancer, anti‑tumor and anti‑inflammatory properties. The present study aimed to investigate the effects of methanolic extracts of gondre on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). As characterized by nuclear magnetic resonance spectroscopy and matrix‑assisted laser deposition/ionization (time‑of‑flight) mass spectrometry, the methanol extract of gondre was found to be enriched with pectolinarin. After 48 h, enhanced viability of hPDLSCs was observed in the presence of gondre compared with under control conditions, suggesting the biocompatibility of gondre. Notably, biocompatibility was markedly affected by gondre concentration in cultured media. Relatively high cell viability was observed in medium containing 0.05% gondre. Furthermore, mineralization was significantly higher in hPDLSCs in the presence of gondre compared with that in control cells, indicating their mineralization potential. Increased expression of various transcription markers, such as collagen 1, runt‑related transcription factor 2, bone sialoprotein and alkaline phosphatase, was also detected when hPDLSCs were stimulated with gondre compared with in the control groups, further confirming the superior osteogenic potential of gondre extract for tissue engineering applications, particularly in bone tissues.
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http://dx.doi.org/10.3892/mmr.2021.11903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893721PMC
April 2021

[Comparison of clinical efficacy of femoral calcar prosthesis replacement and intramedullary nail in the treatment of elderly patients with intertrochanteric fracture].

Zhongguo Gu Shang 2020 Nov;33(11):1017-22

Jinhua Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine, Jinhua 321017, Zhejiang, China.

Objective: To analyze the clinical efficacy of hip arthroplasty with femoral calcar prosthesis and proximal femoral nail fixation(PFNA) in the treatment of elderly patients(≥80 years old) with unstable intertrochanteric fractures(Evans Ⅲ, Ⅳ).

Methods: From June 2016 to March 2018, 60 elderly patients with unstable intertrochanteric fractures treated with prosthetic replacement and PFNA were retrospectively analyzed. According to the surgical methods, they were divided into PFNA group and prosthesis group. In PFNA group there were including 21 males and 15 females, with an average age of(84.3± 2.9) years old;in the prosthetic group, there were 10 males and 14 females with an average age of (82.9±2.4) years old. The operation time, hemoglobin difference between preoperative and postoperative 1 day, postoperative ambulation time, hospitalization time and complications were observed and compared between the two groups. Harris hip score was performed 3 and 12 months after operation.

Results: All patients were followed up for 12 to 24 months (19.3±4.8) months. One patient in the prosthesis group died of lung cancer one year later and the follow-up was terminated. The operation time of prosthetic group was longer than that of PFNA group(<0.05);hemoglobin difference before and after operation had no difference between prosthetic group and PFNA group(>0.05);the time of ambulation in prosthetic group was earlier than that in PFNA group(<0.05); the number of complications in prosthetic group was less than that in PFNA group(<0.05);there was no significant difference in Harris score between prosthetic group and PFNA group before treatment, but scores of both groups were significantly increased after treatment (P <0.05). After 3 months of follow-up, the score of prosthesis group was higher than that of intramedullary nail group(<0.05), but there was no significant difference at 12 months after operation(>0.05).

Conclusion: The elderly patients with intertrochanteric arthroplasty can reduce the burden of intertrochanteric arthroplasty and improve the quality of life.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2020.11.006DOI Listing
November 2020

Serum KIAA1199 is an advanced-stage prognostic biomarker and metastatic oncogene in cholangiocarcinoma.

Aging (Albany NY) 2020 11 10;12(23):23761-23777. Epub 2020 Nov 10.

Department of Surgery, School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Cell proliferation and migration are the determinants of malignant tumor progression, and a better understanding of related genes will lead to the identification of new targets aimed at preventing the spread of cancer. Some studies have shown that KIAA1199 (CEMIP) is a transmembrane protein expressed in many types of noncancerous cells and cancer cells. However, the potential role of KIAA1199 in the progression of cholangiocarcinoma (CCA) remains unclear.

Results: Analysis of cancer-related databases showed that KIAA1199 is overexpressed in CCA. ELISA, immunohistochemistry, Western blotting and qPCR indicated high expression levels of KIAA1199 in serum, CCA tissues and CCA cell lines. In the serum (n = 41) and large sample validation (n = 177) cohorts, higher KIAA1199 expression was associated with shorter overall survival and disease-free survival times. At the cellular level, KIAA1199 overexpression (OE) promoted CCA growth and metastasis. Subcutaneous tumor xenograft experiments showed that KIAA1199 enhances CCA cell proliferation. Additionally, the expression levels of components in the EMT-related TGF-β pathway changed significantly after KIAA1199 upregulation and silencing.

Conclusion: KIAA1199 is a promising new diagnostic molecule and therapeutic target in CCA. The serum KIAA1199 level can be used as a promising clinical tool for predicting the overall postoperative outcomes of patients with CCA.

Methods: CCA-related KIAA1199 data were downloaded from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. To assess the prognostic impact of KIAA1199, an enzyme-linked immunosorbent assay (ELISA) was used to measure the serum level of KIAA1199 in 41 patients who underwent surgical resection. Immunohistochemical staining, Western blotting and qPCR were used to verify and retrospectively review the expression levels of KIAA1199 in cancer tissue specimens from 177 CCA patients. The effect of KIAA1199 on CCA was evaluated by cell-based functional assays and subcutaneous tumor xenograft experiments. The expression levels of proteins associated with epithelial-mesenchymal transition (EMT) and activation of relevant signaling pathways were measured via Western blotting.
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http://dx.doi.org/10.18632/aging.103964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762501PMC
November 2020

Voltage-assisted SILAR deposition of CdSe quantum dots to construct a high performance of ZnS/CdSe/ZnS quantum dot-sensitized solar cells.

J Colloid Interface Sci 2021 Mar 3;586:640-646. Epub 2020 Nov 3.

Key Laboratory of Macromolecular Science of Shaanxi Province, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address:

The charge recombination on the interfaces of TiO/quantum dots (QDs)/electrolyte is a key factor limiting the efficiency of quantum dot-sensitized solar cells (QDSSCs). Construction of double-layer barrier structure of ZnS/QDs/ZnS is a vital strategy to suppress the interfacial charge recombination. However, a large lattice mismatch (12%) at CdSe/ZnS interfaces causes CdSe to grow slowly on TiO/ZnS mesoporous film, weakening the interaction between QDs and mesoporous film, which reducing the efficiency of CdSe QDSSCs with double ZnS barrier layers. Applying a voltage of 2 V in successive ionic layer adsorption reaction (VASILAR) to create an electric field, which assists Cd and SeSO ions rapidly diffuse into the TiO/ZnS mesoporous film to react forming CdSe QDs at room temperature. Optimizing the number of CdSe QDs deposition layers and combine with ZnS double-layer barrier structure, a best PCE of 4.34% for ZnS/CdSe/ZnS QDSSCs is achieved. This study gives a fast and simple approach to inhibit interfacial charge recombination to construct high performance CdSe QDSSCs.
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http://dx.doi.org/10.1016/j.jcis.2020.10.132DOI Listing
March 2021

Applicability of time fractional derivative models for simulating the dynamics and mitigation scenarios of COVID-19.

Chaos Solitons Fractals 2020 Sep 4;138:109959. Epub 2020 Jun 4.

Department of Dermatology, Maternal and Child Health Care Hospital of Qixia District, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210028, China.

Fractional calculus provides a promising tool for modeling fractional dynamics in computational biology, and this study tests the applicability of fractional-derivative equations (FDEs) for modeling the dynamics and mitigation scenarios of the novel coronavirus for the first time. The coronavirus disease 2019 (COVID-19) pandemic radically impacts our lives, while the evolution dynamics of COVID-19 remain obscure. A time-dependent Susceptible, Exposed, Infectious, and Recovered (SEIR) model was proposed and applied to fit and then predict the time series of COVID-19 evolution observed over the last three months (up to 3/22/2020) in China. The model results revealed that 1) the transmission, infection and recovery dynamics follow the integral-order SEIR model with significant spatiotemporal variations in the recovery rate, likely due to the continuous improvement of screening techniques and public hospital systems, as well as full city lockdowns in China, and 2) the evolution of number of deaths follows the time FDE, likely due to the time memory in the death toll. The validated SEIR model was then applied to predict COVID-19 evolution in the United States, Italy, Japan, and South Korea. In addition, a time FDE model based on the random walk particle tracking scheme, analogous to a mixing-limited bimolecular reaction model, was developed to evaluate non-pharmaceutical strategies to mitigate COVID-19 spread. Preliminary tests using the FDE model showed that self-quarantine may not be as efficient as strict social distancing in slowing COVID-19 spread. Therefore, caution is needed when applying FDEs to model the coronavirus outbreak, since specific COVID-19 kinetics may not exhibit nonlocal behavior. Particularly, the spread of COVID-19 may be affected by the rapid improvement of health care systems which may remove the memory impact in COVID-19 dynamics (resulting in a short-tailed recovery curve), while the death toll and mitigation of COVID-19 can be captured by the time FDEs due to the nonlocal, memory impact in fatality and human activities.
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http://dx.doi.org/10.1016/j.chaos.2020.109959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7269951PMC
September 2020

Comparing different kidney stone scoring systems for predicting percutaneous nephrolithotomy outcomes: A multicenter retrospective cohort study.

Int J Surg 2020 Sep 29;81:55-60. Epub 2020 Jul 29.

Department of Urology, Beijing Hospital; National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, China; Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China. Electronic address:

Objective: To compare the predictive performance of five previously described scoring systems (i.e., S.T.O.N.E., Guy's, Clinical Research Office of the Endourological Society (CROES), the Seoul National University Renal Stone Complexity (S-RESC) and the new Stone Kidney Size (SKS) score) for postoperative outcomes regarding stone-free rate (SFR) and complications in adult patients.

Methods: Data from 349 patients who underwent percutaneous nephrolithotomy (PCNL) in three urology departments were analyzed. SKS, S.T.O.N.E., S-ReSC, CROES and Guy's nephrolithometry scoring systems were used to retrospectively calculate predictions for each patient. Univariate and multivariate analyses were performed to evaluate factors associated with SFR and complication rates. Receiver operating characteristic (ROC) curves were generated and areas under curves (AUC) were compared to identify the method with the highest predictive value.

Results: Median SKS, S.T.O.N.E., S-ReSC, CROES and Guy's scores were 4, 7, 3, 170.8 and 2, respectively. Overall, SFR was 67.0% (234/349) with a complications rate of 36.7% (128/349). AUCs of each method for predicting stone-free status, highlighted reasonable predictive capabilities with 0.709, 0.806, 0 0.869, 0.207, and 0.735, respectively; however, the S-ReSC scoring system had the best discriminative performance. According to multivariate logistic regression and AUC results, none were effectively capable of predicting complications.

Conclusions: All scoring systems correlated significantly with stone-free status; although, S-ReSC appears to have the greatest predictive ability. This method is also relatively easy to implement and highly reproducible. However, none of the methods analyzed are able to accurately predict postoperative complications.
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http://dx.doi.org/10.1016/j.ijsu.2020.07.025DOI Listing
September 2020

Osteogenic potential of human mesenchymal stem cells on eggshells-derived hydroxyapatite nanoparticles for tissue engineering.

J Biomed Mater Res B Appl Biomater 2020 07 10;108(5):1953-1960. Epub 2019 Dec 10.

Department of Biosystems Engineering, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Republic of Korea.

Nanocrystalline hydroxyapatite (HAp) was synthesized from biowaste eggshells through sonication followed by the heat treatment. Calcium oxide as a precursor moiety for the synthesis of HAp was obtained through the heat treatment of eggshells at 900°C for 3 hr. The prepared HAp was characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM). The appearance of the FTIR absorption peaks in between at 516-1031 and 3,636 cm shows phosphate and hydroxyl groups in prepared HAp, respectively. The XRD-patterns indicate the formation of HAp started within 5 min of sonication. The SEM morphologies suggested that the synthesized HAp was highly crystalline and compact. We tested the elemental analysis of the synthesized HAp through X-ray fluorescence spectroscopy and inductively coupled plasma mass spectroscopy. The higher Ca/P ratio has observed in heat-treated HAp. These results show that heat treatment facilitates the formation of highly crystalline and compact HAp. Cytotoxicity and osteogenic potential of human mesenchymal stem cells (hMSCs) were also evaluated in the presence of HAp. No significant cytotoxicity was noted in the presence of HAp, suggested their biocompatibility. Enhanced osteogenesis of hMSCs occurred with HAp powder, confirming the feasibility in the treatment of osteogenesis. Thus, synthesized HAp has the potential to use a biomaterial in tissue engineering applications for bone tissues.
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http://dx.doi.org/10.1002/jbm.b.34536DOI Listing
July 2020

Overlay of a sponge soaked with ropivacaine and multisite infiltration analgesia result in faster recovery after laparoscopic hepatectomy.

World J Gastroenterol 2019 Sep;25(34):5185-5196

Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.

Background: Compared with traditional open surgery, laparoscopic surgery is preferred due to the advantages of less trauma, less pain, and faster recovery. Nevertheless, many patients still suffer from postoperative pain resulting from the surgical incision and associated tissue injury. Many researchers have reported methods to improve postoperative pain control, but there is not a simple and effective method that can be clinically adopted in a widespread manner. We designed this study to prove the hypothesis that application of ropivacaine in the port site and operative site in patients is an effective and convenient method which can decrease postoperative pain and accelerate recovery.

Aim: To evaluate the effects of ropivacaine on pain control after laparoscopic hepatectomy and its contribution to patient recovery.

Methods: From May 2017 to November 2018, 146 patients undergoing laparoscopic hepatectomy were randomized to receive infiltration of either 7.5 mg/mL ropivacaine around the trocar insertions, incision, and cutting surface of the liver (with a gelatin sponge soaked with ropivacaine) at the end of surgery (ropivacaine group), or normal saline (5 mL) at the same sites at the end of surgery (control group). The degree of pain, nausea, vomiting, heart rate (HR), and blood pressure were collected. The length of postoperative hospitalization, complications, and the levels of stress hormones were also compared between the two groups.

Results: Compared with the control group, the ropivacaine group showed reduced postoperative pain at rest within 12 h ( < 0.05), and pain on movement was reduced within 48 h. The levels of epinephrine, norepinephrine, and cortisol at 24 and 48 h, HR, blood pressure, and cumulative sufentanil consumption in the ropivacaine group were significantly lower than those in the control group ( < 0.05). In the ropivacaine group, hospitalization after operation was shorter, but the difference was not statistically significant. There were no significant differences in postoperative nausea, vomiting, or other complications, including hydrothorax, ascites, peritonitis, flatulence, and venous thrombus ( > 0.05), although fewer patients in the ropivacaine group experienced these situations.

Conclusion: Infiltration with ropivacaine in the abdominal wound and covering the cutting surface of the liver with a gelatin sponge soaked with ropivacaine significantly reduce postoperative pain and the consumption of sufentanil.
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http://dx.doi.org/10.3748/wjg.v25.i34.5185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747283PMC
September 2019

KLF5 downregulation desensitizes castration-resistant prostate cancer cells to docetaxel by increasing BECN1 expression and inducing cell autophagy.

Theranostics 2019 28;9(19):5464-5477. Epub 2019 Jul 28.

Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China, 710061.

KLF5 is frequently deleted or downregulated in prostate cancer. However, it is not known whether downregulation of KLF5 is associated with the response of prostate cancer cells to chemotherapy and/or prognosis of patients. We monitored cell growth by MTT and colony formation assays, and cell autophagy through tandem fluorescence microscopy and transmission electron microscopy. Gene expression was analyzed by RT-qPCR and Western blotting. We determined the binding of KLF5 together with HDAC3 on the beclin-1 () promoter by the ChIP assay, oligonucleotides pulldown, and co-immunoprecipitation. The effect of docetaxel on cell growth was examined in a CWR22RV1 xenograft tumor mouse model. : In the present study, we found that KLF5 down-regulation was associated with progression of prostate cancer and poor prognosis of patients. KLF5 knockdown reduced the sensitivity of prostate cancer cells to docetaxel and , and docetaxel treatment decreased the expression of KLF5. Moreover, we confirmed that docetaxel treatment inhibited cell death by inducing autophagy in prostate cancer cells. Thus, we hypothesized that KLF5 could be a regulator of cell autophagy. Interestingly, KLF5 could inhibit prostate cancer cell autophagy by suppressing the transcription of BECN1 cooperatively with HDAC3. Another significant finding was that docetaxel treatment repressed KLF5 expression through AMPK/mTOR/p70S6K signaling pathway resulting in increased BECN1, induction of cell autophagy, and promotion of cell survival in castration-resistant prostate cancer cells. Our results indicated that downregulation of KLF5 promoted cell autophagy in prostate cancer. Furthermore, reduced KLF5 also facilitated cell autophagy induced by docetaxel resulting in desensitization to the drug and cell survival. Decreased levels of KLF5 led to increased BECN1 via AMPK/mTOR/p70S6K signaling. Thus, repression of BECN1 and cell autophagy was critical for KLF5 to increase the sensitivity of prostate cancer cells to docetaxel.
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http://dx.doi.org/10.7150/thno.33282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735397PMC
September 2020

A novel technique to prepare a single cell suspension of isolated quiescent human hepatic stellate cells.

Sci Rep 2019 09 4;9(1):12757. Epub 2019 Sep 4.

Department of general surgery, Qilu hospital of Shandong University, Jinan, China.

To explore a simple and easy-to-learn procedure for the isolation of human quiescent hepatic stellate cells (HSCs) that requires no advanced training. Thus reducing costs and increasing efficiency. This protocol will provide sufficient primary cells with minimal contaminants for future basic research on diseases associated with human HSCs. Normal liver tissues were isolated from patients undergoing hepatic hemangioma resection, and a single cell suspension of these tissues was prepared using the Gentle MACS tissue processor. By using this method, the difficulty of the procedure was reduced, fewer cells were lost during the preparation treatments, and the maximal activity of single cells was maintained. Following preparation of the cell suspension, the HSCs were further isolated using a Nycodenz density gradient. Cell viability was examined by trypan blue staining, and the purity of the quiescent human HSCs was determined by autofluorescence and oil red O staining. Activated and quiescent human HSCs were identified using immunofluorescence and Western blotting. The cell cycle distribution in activated and quiescent human HSCs was analyzed by flow cytometry.The recovery rate of the HSCs was approximately (2.1 ± 0.23) × 10 of tissue, with 94.43 ± 1.89% cell viability and 93.8 ± 1.52% purity. The technique used in this study is a simple, high-yield, and repeatable method for HSC isolation that is worthy of recommendation.
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http://dx.doi.org/10.1038/s41598-019-49287-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726602PMC
September 2019

Is laparoscopic hepatectomy suitable for giant hepatic hemangioma larger than 10 cm in diameter?

Surg Endosc 2020 03 3;34(3):1224-1230. Epub 2019 Jun 3.

Liver Surgery Center, Shandong University Qilu Hospital, Jinan, China.

Background: Surgical treatment of giant hepatic hemangioma larger than 10 cm in diameter often requires major laparoscopic liver resection (LLR), which is very difficult and has a high risk of bleeding. There is no consensus as to whether giant hepatic hemangioma is an indication or a contraindication for laparoscopic surgery.

Methods: From 2011/09 to 2018/05, 58 patients with giant hepatic hemangioma larger than 10 cm underwent laparoscopic surgery. They were divided into high-difficulty (HD) group and low-difficulty (LD) group according to the operation difficulty score. The perioperative data of the two groups were analyzed and compared.

Results: There were 30 patients in the LD group and 28 in the HD group. No differences were showed in sex, age, ASA grade, tumor size, operation time, conversion rate, intraoperative and post-operative blood transfusion rate, and post-operative complications between the two groups. The amount of bleeding in the HD group was significantly higher than that in the LD group (285.7 ± 224.0 vs 189.6 ± 157.6 ml, p = 0.007). The time of hepatic hilar occlusion in the HD group was longer than that in the LD group (25.4 ± 8.8 vs 20.6 ± 7.3 min, p = 0.001), and the rate of hepatic hilar occlusion in the HD group was also higher than that in the LD group (71.4% vs 33.3%, p = 0.004). The incidence of hospitalization days and discharge time in the LD group were shorter than those in the HD group (6.3 ± 1.6 vs 8.2 ± 1.4 days, p = 0.000; 16.4 ± 5.5 vs 21.9 ± 4.6 h, p = 0.019). There was no significant difference in enzymatic parameters between the two groups after operation.

Conclusions: Although there are great difficulties and risks objectively, laparoscopic treatment of giant hepatic hemangioma is safe and feasible.
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http://dx.doi.org/10.1007/s00464-019-06880-1DOI Listing
March 2020

Enhanced Osteogenesis of Human Mesenchymal Stem Cells in Presence of Single-Walled Carbon Nanotubes.

IEEE Trans Nanobioscience 2019 07 2;18(3):463-468. Epub 2019 May 2.

Human mesenchymal stem cells (hMSCs) have attracted significant attention for tissue engineering because of their ability to differentiate into bone cells, chondrocytes, adipocytes, and muscle cells. Single-walled carbon nanotubes (SWCNTs) have been considered as a potential material for tissue engineering applications due to their unique properties, such as high aspect ratio, excellent electrocatalytic activity, and biocompatibility. Here we prepared exfoliated SWCNTs layers through an ultra-sonication process in the acidic medium and evaluated their cytotoxicity using hMSCs. Improved viability and osteogenesis of hMSCs were observed in the presence of exfoliated SWCNTs. Besides, the higher expression of osteogenic differentiation-related genes in the presence of exfoliated SWCNTs further confirmed their enhanced osteogenic nature. These results indicated the potential of SWCNTs as a biomaterial for tissue engineering applications.
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http://dx.doi.org/10.1109/TNB.2019.2914127DOI Listing
July 2019

Expression patterns and the prognostic value of the SMYD family members in human breast carcinoma using integrative bioinformatics analysis.

Oncol Lett 2019 Apr 19;17(4):3851-3861. Epub 2019 Feb 19.

Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250112, P.R. China.

Suppressor of variegation, Enhancer of Zeste, Trithorax and Myeloid-Nervy-DEAF1 domain-containing (SMYD) proteins are a set of lysine methyltransferases involved in a range of diverse biological functions, including gene expression, and regulation of skeletal and cardiac-muscle development. These proteins may additionally serve roles in a number of different types of cancer. However, the roles of the five SMYD proteins, SMYD 1/2/3/4/5, their expression patterns and prognostic value remain unclear. In the present study, the transcriptional expression levels of the five SMYD proteins were compared with the survival data of patients with breast carcinoma (BC) from the ONCOMINE dataset, Breast Cancer Gene-Expression Miner v4.0, Kaplan-Meier Plotter, The Cancer Genome Atlas and cBioPortal. An increase in the SMYD2/3/5 mRNA expression levels and a decrease in SMYD1/4 mRNA expression levels in BC tissues compared with normal tissues were identified. Increased SMYD3 mRNA and decreased SMYD5 mRNA expression levels were associated with decreased levels of histological differentiation, according to the Scarff-Bloom-Richardson grading system. Kaplan-Meier curves demonstrated that the increased SMYD1/4 and decreased SMYD2/3 mRNA expression levels were associated with good relapse-free survival (RFS) in patients with BC. Furthermore, SMYD2 mRNA expression levels were associated with the RFS of patients with BC with metastatic relapse, and SMYD4 may serve as a tumor suppressor in patients with BC, as patients with increased SMYD4 mRNA expression levels had significantly better RFS compared with decreased SMYD4 mRNA expression levels. The present data suggested that SMYD2 and SMYD3 may be potential biomarkers for diagnosis of BC. Additionally, SMYD2 and SMYD4 may be potential prognostic indicators of patients with BC.
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http://dx.doi.org/10.3892/ol.2019.10054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425337PMC
April 2019

Identifying heavy metal pollution hot spots in soil-rice systems: A case study in South of Yangtze River Delta, China.

Sci Total Environ 2019 Mar 11;658:614-625. Epub 2018 Dec 11.

Institute of Agricultural Remote Sensing and Information Technology Application, Zhejiang University, Hangzhou 310058, China. Electronic address:

The soil-rice system in China is subjected to increasing concentrations of heavy metals (HMs) which derived from various sources. It is very critical to investigate the concentrations, spatial characteristics and hot spots of HMs content in the soil-rice system. This study presents work completed on 915 soil-rice sample pairs collected from South of Yangtze River Delta, China. These samples were evaluated for HM concentrations. Ordinary Kriging and the Getis-Ord index were used to explore spatial distributions and pollution hot spots. Averaged HMs content in soil is shown to be Zn > Cr > Pb > Cu > Ni > As > Hg > Cd, and concentrations in rice arrange as Zn > Cu > Cr > Ni > As > Cd > Pb > Hg. Compared with Chinese maximum permissible limits, mean content of all HMs in farmland soil are at safe levels and averaged content of all HMs in rice were also at safe levels except As and Ni. Ni was most polluted HM in soil Most of and showed relatively high content in farmland soil in southeastern part. As and Ni are the most polluted in rice, with highest content distributed in the northwestern and southern area, respectively. The majority of HMs pollution hot spots in soil clustered in the central area. Pollution hot spots of Ni and As in rice are mainly concentrated in the central part and southeastern part, correspondingly. Our results found a weak link between content and spatial pattern of pollution status of HMs in soil and rice. The results are anticipated to contribute to more efficient and accurate control of HMs pollution in soil-rice system, and assist decision-makers achieve a balance between cost and regulation of HM pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2018.12.150DOI Listing
March 2019

KIAA1199 overexpression is associated with abnormal expression of EMT markers and is a novel independent prognostic biomarker for hepatocellular carcinoma.

Onco Targets Ther 2018 23;11:8341-8348. Epub 2018 Nov 23.

Department of General Surgery, Qilu Hospital of Shandong University, Jinnan, China,

Purpose: To determined KIAA1199 expression and investigate its correlation with the clinicopathologic data and prognosis of hepatocellular carcinoma (HCC), as well as markers of epithelial-mesenchymal transition (EMT); N-cadherin, E-cadherin and vimentin.

Materials And Methods: Western blot, quantitative real-time PCR, and immunohistochemical staining were used to measure KIAA1199 expression in human HCC specimens. Subsequently, the correlation between KIAA1199 expression and the pathological characteristics of HCC patients was analyzed. Univariate and multivariate analyses were used to explore the risk factors associated with disease-free survival (DFS) and overall survival (OS).

Results: KIAA1199 expression was remarkably increased in hepatocellular carcinoma tissues compared to paracarcinomatous tissues. This phenomenon was accompanied by aberrant expression of EMT-associated markers. In addition, high KIAA1199 expression was associated with severe pathological symptoms, low DFS, and low OS. Results of the multivariate analysis showed that KIAA1199 expression may be an independent predictor of low disease-free survival and OS of HCC patients.

Conclusion: KIAA1199 overexpression in HCC patients is associated with aberrant expression of EMT-associated markers and severe clinicopathological symptoms, and thus may function as a marker of poor prognosis in HCC.
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http://dx.doi.org/10.2147/OTT.S187389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260188PMC
November 2018

Liver Retraction Using n-Butyl-2-Cyanoacrylate (NBCA) Glue during Laparoscopic Splenectomy and Azygoportal Disconnection in Cirrhotic Patients.

Biomed Res Int 2018 19;2018:3064046. Epub 2018 Aug 19.

Department of General Surgery, Qilu Hospital of Shan Dong University, Jinan 250012, China.

Background: Although liver retraction using n-butyl-2-cyanoacrylate (NBCA) glue has been applied to laparoscopic upper abdominal surgery in noncirrhotic patients, there is still no consensus on its safety and feasibility for cirrhotic patients. In this study, we aimed to investigate the safety and effectiveness of liver retraction using NBCA glue during laparoscopic splenectomy and azygoportal disconnection (LSD) for gastroesophageal varices and hypersplenism secondary to liver cirrhosis and portal hypertension.

Methods: Thirty-nine gastroesophageal varices and hypersplenism secondary to liver cirrhosis and portal hypertension patients were included in our study. We performed LSD in the presence of NBCA glue (n = 22, NBCA group) and absence of NBCA glue (n = 17, n-NBCA group), respectively. The operation time, blood loss, postoperative hospitalization, and liver function were compared between the two groups.

Results: There was no mortality during the operation. One patient in non-NBCA group received open surgery due to parenchyma hemorrhage. Postoperative pleural effusion occurred in 2 cases of the NBCA group and 1 of the non-NBCA group. One showed left subphrenic abscess in the non-NBCA group. No postoperative bleeding occurred after 9-30 months of follow-up. The time of operation in NBCA group was significantly shorter than those in n-NBCA group (198.86±17.86 versus 217.81±20.25min, P<0.01). Blood loss in NBCA group was significantly lower than non-NBCA group (159.09±56.98 versus 212.50±88.51 ml, P<0.05). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased on day 1 after LSD and decreased to normal level on day 7 after LSD in both groups. There was no significant difference in postoperative hospitalization and liver function between the two groups.

Conclusion: Liver retraction using NBCA glue during LSD for gastroesophageal varices and hypersplenism secondary to liver cirrhosis and portal hypertension is safe, effective, and feasible.
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http://dx.doi.org/10.1155/2018/3064046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6120335PMC
January 2019

Source Identification and Apportionment of Trace Elements in Soils in the Yangtze River Delta, China.

Int J Environ Res Public Health 2018 06 12;15(6). Epub 2018 Jun 12.

Institute of Applied Remote Sensing and Information Technology, Zhejiang University, Hangzhou 310058, China.

Trace elements pollution has attracted a lot of attention worldwide. However, it is difficult to identify and apportion the sources of multiple element pollutants over large areas because of the considerable spatial complexity and variability in the distribution of trace elements in soil. In this study, we collected total of 2051 topsoil (0⁻20 cm) samples, and analyzed the general pollution status of soils from the Yangtze River Delta, Southeast China. We applied principal component analysis (PCA), a finite mixture distribution model (FMDM), and geostatistical tools to identify and quantitatively apportion the sources of seven kinds of trace elements (chromium (Cr), cadmium (Cd), mercury (Hg), copper (Cu), zinc (Zn), nickel (Ni), and arsenic (As)) in soil. The PCA results indicated that the trace elements in soil in the study area were mainly from natural, multi-pollutant and industrial sources. The FMDM also fitted three sub log-normal distributions. The results from the two models were quite similar: Cr, As, and Ni were mainly from natural sources caused by parent material weathering; Cd, Cu, and Zu were mainly from mixed sources, with a considerable portion from anthropogenic activities such as traffic pollutants, domestic garbage, and agricultural inputs, and Hg was mainly from industrial wastes and pollutants.
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http://dx.doi.org/10.3390/ijerph15061240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025603PMC
June 2018

A systematic surgical procedure: The '7+3' approach to laparoscopic right partial hepatectomy [deep segment (S) VI, S VII or S VIII] in 52 patients with liver tumors.

Oncol Lett 2018 May 23;15(5):7846-7854. Epub 2018 Mar 23.

Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

Laparoscopic right partial hepatectomy (LRPH), located in the deep segment (S) VI, S VII or S VIII, is a complicated procedure, due to its poor operative field and high risk of bleeding. The present study aimed to summarize our experience of LRPH and to share our systematic surgical procedure, the '7+3' approach. This approach includes seven key points and three main instruments. A total of 81 cases were included, which were divided into 2 groups [LRPH, n=52; open hepatectomy (OH), n=29]. The demographic profile, intraoperative parameters and postoperative parameters were obtained and analyzed. Blood loss (245.38±268.37 ml) in the LRPH group was not significantly more than in the OH group (230.93±257.62 ml; P=0.936). The durations of surgery, liver parenchyma transection and portal triad clamping were also not significantly more than those in the OH group (145.52±48.29 vs. 129.83±35.04 min; P=0.149 for surgery; 28.52±10.16 vs. 23.97±10.44 min; P=0.059 for liver parenchyma transection; 20.62±9.61 vs. 17.31±10.12 min; P=0.149 for portal triad clamping). However, the number of postoperative hospital days in the LRPH group was smaller (10.67 in LRPH vs. 12.07 in OH; P=0.025). The present study demonstrated the satisfactory surgical outcomes and economic benefits of the systematic '7+3' surgical technique for LRPH. Further studies in larger cohorts and other centers are required to confirm its feasibility and superiority.
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http://dx.doi.org/10.3892/ol.2018.8345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5962865PMC
May 2018

Heavy Metal Pollution Delineation Based on Uncertainty in a Coastal Industrial City in the Yangtze River Delta, China.

Int J Environ Res Public Health 2018 04 10;15(4). Epub 2018 Apr 10.

Institute of Agricultural Remote Sensing and Information Technology Application, Zhejiang University, Hangzhou 310058, China.

Assessing heavy metal pollution and delineating pollution are the bases for evaluating pollution and determining a cost-effective remediation plan. Most existing studies are based on the spatial distribution of pollutants but ignore related uncertainty. In this study, eight heavy-metal concentrations (Cr, Pb, Cd, Hg, Zn, Cu, Ni, and Zn) were collected at 1040 sampling sites in a coastal industrial city in the Yangtze River Delta, China. The single pollution index (PI) and Nemerow integrated pollution index (NIPI) were calculated for every surface sample (0-20 cm) to assess the degree of heavy metal pollution. Ordinary kriging (OK) was used to map the spatial distribution of heavy metals content and NIPI. Then, we delineated composite heavy metal contamination based on the uncertainty produced by indicator kriging (IK). The results showed that mean values of all PIs and NIPIs were at safe levels. Heavy metals were most accumulated in the central portion of the study area. Based on IK, the spatial probability of composite heavy metal pollution was computed. The probability of composite contamination in the central core urban area was highest. A probability of 0.6 was found as the optimum probability threshold to delineate polluted areas from unpolluted areas for integrative heavy metal contamination. Results of pollution delineation based on uncertainty showed the proportion of false negative error areas was 6.34%, while the proportion of false positive error areas was 0.86%. The accuracy of the classification was 92.80%. This indicated the method we developed is a valuable tool for delineating heavy metal pollution.
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http://dx.doi.org/10.3390/ijerph15040710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923752PMC
April 2018

Surgical Technique and Clinical Analysis of Twelve Cases of Isolated Laparoscopic Resection of the Hepatic Caudate Lobe.

Biomed Res Int 2018 16;2018:5848309. Epub 2018 Jan 16.

Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.

Objective: To describe the surgical procedures of laparoscopic caudate lobectomy and analyze its clinical efficiency for treating cancer.

Methods: Twelve consecutive patients of hepatocellular carcinoma, hepatic hemangioma, and focal nodular hyperplasia who received laparoscopic caudate lobectomy in Qilu Hospital of Shandong University from January 2013 to January 2017 were included in this study. The clinical data, intraoperative parameters, and postoperative outcomes were assessed.

Results: All 12 patients received totally laparoscopic technique. The operative time was 140.8 ± 95.34 minutes. The average estimated blood loss was 97.92 ± 90.54 ml, and no blood transfusions were required. The mean duration of hospital stay was 9.17 ± 2.88 days. There was no perioperative complication or patient mortality in this series.

Conclusions: Laparoscopic caudate lobectomy is safe and feasible in the selected patients.
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http://dx.doi.org/10.1155/2018/5848309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5820552PMC
September 2018

Salen-Mn compounds induces cell apoptosis in human prostate cancer cells through promoting AMPK activity and cell autophagy.

Oncotarget 2017 Oct 21;8(49):86277-86286. Epub 2017 Sep 21.

Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Currently only docetaxel has been approved to be used in the chemotherapy of prostate cancer and new drugs are urgent need. Salen-Mn is a novel type of synthetic reagent bionic and exerts remarkable anticancer activities. However, the effect of Salen-Mn on human prostate cancer has not been elucidated yet. In this study, we found that treatment of PC-3 and DU145 human prostate cancer cells with Salen-Mn inhibited cell growth in dose and time dependent manner. Moreover, Salen-Mn induced cell apoptosis, and increased the expression of apoptotic proteins, such as cleaved caspase-3, cleaved PARP, and Bax, in PC-3 and DU145 prostate cancer cells. Furthermore, we found that Salen-Mn induced expression of LC3-I/II, which is protein marker of cell autophagy, in both dose and time dependent manners; in addition, Salen-Mn increased the phosphorylation of AMPK, suggesting that Salen-Mn increase cell autophagy through activating AMPK pathway. On the other hand, when PC-3 and DU145 cells were treated with Salen-Mn and 3-MA, an inhibitor of cell autophagy, the inhibitory effect of Salen-Mn on cell growth and the induction of apoptotic proteins were decreased. In addition, we found that Salen-Mn inhibited the growth of PC-3 cell xenografts in nude mice. In summary, our results indicate that Salen-Mn suppresses cell growth through inducing AMPK activity and autophagic cell death related cell apoptosis in prostate cancer cells and suggest that Salen-Mn and its derivatives could be new options for the chemical therapeutics in the treatment of prostate cancer.
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http://dx.doi.org/10.18632/oncotarget.21138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689684PMC
October 2017

Effect of local wound infiltration with ropivacaine on postoperative pain relief and stress response reduction after open hepatectomy.

World J Gastroenterol 2017 Sep;23(36):6733-6740

Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.

Aim: To prospectively evaluate the effect of local wound infiltration with ropivacaine on postoperative pain relief and stress response reduction after open hepatectomy.

Methods: A total of 56 patients undergoing open hepatectomy were randomly divided into two groups: a ropivacaine group (wound infiltration with ropivacaine solution) and a control group (infiltration with isotonic saline solution). A visual analog scale (VAS) at rest and on movement was used to measure postoperative pain for the first 48 h after surgery. Mean arterial pressure (MAP), heart rate (HR), time to bowel recovery, length of hospitalization after surgery, cumulative sufentanil consumption, and incidence of nausea and vomiting were compared between the two groups. Surgical stress hormones (epinephrine, norepinephrine, and cortisol) were detected using enzyme-linked immunosorbent assay, and the results were compared.

Results: VAS scores both at rest and on movement at 24 h and 48 h were similar between the two groups. Significantly lower VAS scores were detected at 0, 6, and 12 h in the ropivacaine group compared with the control group ( < 0.05 for all). MAP was significantly lower at 6, 12, and 24 h ( < 0.05 for all); HR was significantly lower at 0, 6, 12, and 24 h ( < 0.05 for all); time to bowel recovery and length of hospitalization after surgery ( < 0.05 for both) were significantly shortened; and cumulative sufentanil consumption was significantly lower at 6, 12, 24, and 36 h ( < 0.05 for all) in the ropivacaine group than in the control group, although the incidence of nausea and vomiting showed no significant difference between the two groups. The levels of epinephrine, norepinephrine, and cortisol were significantly lower in the ropivacaine group than in the control group at 24 and 48 h ( < 0.01 for all).

Conclusion: Local wound infiltration with ropivacaine after open hepatectomy can improve postoperative pain relief, reduce surgical stress response, and accelerate postoperative recovery.
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http://dx.doi.org/10.3748/wjg.v23.i36.6733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643294PMC
September 2017

Adjuvant transarterial chemoembolization after radical resection contributed to the outcomes of hepatocellular carcinoma patients with high-risk factors.

Medicine (Baltimore) 2017 Aug;96(33):e7426

Department of General Surgery, Qilu Hospital of Shandong University School of Medicine, Shandong University, Jinan Department of General Surgery, Renmin Hospital of Lingcheng, Dezhou, China.

We aim to investigate the effects of postoperative adjuvant transarterial chemoembolization (TACE) on survival and recurrence in hepatocellular carcinoma (HCC) patients after radical resection. A total of 320 HCC patients underwent radical resection between January 2010 and January 2014 in Qilu Hospital, Shandong University were divided into 4 groups according to the frequency of postoperative adjuvant TACE. Patients were further stratified into subgroups (tumor diameter ≤5 or >5 cm) with low or high risk factors for recurrence or death. A low risk factor for recurrence or death was defined as Edmondson grade I/II without microvascular invasion (MiVI), while a high risk factor was defined as Edmondson grade III/IV or with MiVI. Survival data and recurrence rates were compared using the Kaplan-Meier method. Uni- and multivariate analyses were based on the Cox proportional analysis. Compared to those received no TACE, patients underwent 2 (log-rank, χ = 9.054, P = .003) or 3 (log-rank, χ = 4.228, P = .04) TACE showed delayed recurrence. Patients received 2 or 3 TACE showed extended overall survival (OS) compared with the other patients. No statistical differences were found between all the disease-free survival (DFS) and OS in low-risk subgroups. In the patients of the high-risk subgroup with a tumor diameter of ≤5, those received 2 TACE showed delayed recurrence compared with those received no TACE, and TACE (twice or thrice) can improve OS. For those of the high-risk subgroup with a tumor diameter of >5, TACE (twice or thrice) can delay recurrence and improve OS. Adjuvant TACE (twice or thrice) after radical resection is beneficial for HCC patients with poor differentiation and MiVI, especially for those with a tumor diameter of >5 cm.
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http://dx.doi.org/10.1097/MD.0000000000007426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5571673PMC
August 2017

Assessment of the potential health risks of heavy metals in soils in a coastal industrial region of the Yangtze River Delta.

Environ Sci Pollut Res Int 2017 Aug 7;24(24):19816-19826. Epub 2017 Jul 7.

Institute of Agricultural Remote Sensing and Information Technology Application, Zhejiang University, Hangzhou, 310029, China.

Soil heavy metal contamination is a serious environmental problem. Human beings may be directly exposed to heavy metals in soils through the inhalation of soil particles, dermal contact, and oral ingestion, which can seriously threaten health. This study assesses the health risks associated with heavy metals in soils by determining the concentrations of eight heavy metals (Cr, Pb, Cd, Hg, As, Cu, Zn, and Ni) based on 2051 surface-soil samples collected from the southern Yangtze River Delta of China. The mean concentrations were higher than the corresponding background values in Zhejiang Province and China as a whole, indicating an accumulation of heavy metals. The health risk assessment suggests that the non-carcinogenic and carcinogenic risks in the study area were not significant. The non-carcinogenic risk for children was the highest, followed by those for adults and seniors; the non-carcinogenic risk for the entire population was less than 1.0, the predetermined threshold. Carcinogenic risk for adults was the highest, followed by those for seniors and children; a few sample points had a value larger than the threshold of 1.0E-04. Arsenic represented the greatest contribution to non-carcinogenic and carcinogenic risk. Meanwhile, ingestion of heavy metals in soil was the main exposure pathway for carcinogenic risk, followed by inhalation and dermal exposure. The spatial method of Getis-Ord was used to identify hot spots of health risk. Hot spots with high hazard index (HI) and total carcinogenic risk (TCR) for children, adults, and seniors were mainly distributed in core urban areas, such as Jiangbei, Haishu, Yinzhou, Jiangdong, and the urban areas of some other counties, which coincided with industrial, mining, and urban areas of the study area and were strongly influenced by anthropogenic activities. These results provide a basis for heavy metal control in soil, source identification, and environment management in the Yangtze River Delta and other rapidly developing industrial regions in China.
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http://dx.doi.org/10.1007/s11356-017-9516-1DOI Listing
August 2017