Publications by authors named "Bin Hu"

1,883 Publications

  • Page 1 of 1

Iron quantitative analysis of motor combined with bulbar region in M1 cortex may improve diagnosis performance in ALS.

Eur Radiol 2022 Aug 11. Epub 2022 Aug 11.

Department of Radiology, Huashan Hospital, Fudan University, No. 12 Middle Wulumuqi Road, Jingan District, Shanghai, 200040, China.

Objectives: To explore whether the combined analysis of motor and bulbar region of M1 on susceptibility-weighted imaging (SWI) can be a valid biomarker for amyotrophic lateral sclerosis (ALS).

Methods: Thirty-two non-demented ALS patients and 35 age- and gender-matched healthy controls (HC) were retrospectively recruited. SWI and 3D-T1-MPRAGE images were obtained from all individuals using a 3.0-T MRI scan. The bilateral posterior band of M1 was manually delineated by three neuroradiologists on phase images and subdivided into the motor and bulbar regions. We compared the phase values in two groups and performed a stratification analysis (ALSFRS-R score, duration, disease progression rate, and onset). Receiver operating characteristic (ROC) curves were also constructed.

Results: ALS group showed significantly increased phase values in M1 and the two subregions than the HC group, on the all and elderly level (p < 0.001, respectively). On all-age level comparison, negative correlations were found between phase values of M1 and clinical score and duration (p < 0.05, respectively). Similar associations were found in the motor region (p < 0.05, respectively). On both the total (p < 0.01) and elderly (p < 0.05) levels, there were positive relationships between disease progression rate and M1 phase values. In comparing ROC curves, the entire M1 showed the best diagnostic performance.

Conclusions: Combining motor and bulbar analyses as an integral M1 region on SWI can improve ALS diagnosis performance, especially in the elderly. The phase value could be a valuable biomarker for ALS evaluation.

Key Points: • Integrated analysis of the motor and bulbar as an entire M1 region on SWI can improve the diagnosis performance in ALS. • Quantitative analysis of iron deposition by SWI measurement helps the clinical evaluation, especially for the elderly patients. • Phase value, when combined with the disease progression rate, could be a valuable biomarker for ALS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00330-022-09045-2DOI Listing
August 2022

STUB1-SMYD2 Axis Regulates Drug Resistance in Glioma cells.

J Mol Neurosci 2022 Aug 8. Epub 2022 Aug 8.

Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Zhejiang Province, Jinhua, 321000, China.

SET and MYND domain-containing protein 2 (SMYD2) is an important epigenetic regulator that methylates histone and non-histone proteins. The study aimed to investigate the oncogenic role of SMYD2 in gliomas and explore its degradation mechanism induced by cisplatin. Tumor tissue microarray of 441 patients with glioma was collected for SMYD2 immunohistochemical staining. Kaplan-Meier survival curves were constructed using the overall survival values. mRNA-sequencing analysis was performed for understanding the downstream mechanisms mediated by SMYD2. The half-inhibitory concentrations (IC50) of temozolomide and cisplatin in AZ505-treated and control cells were calculated. The potential E3 ubiquitin ligase of SMYD2 was predicted in UbiBrowser and confirmed by a knockdown test. The effect of SMYD2 and its E3 ligase on apoptosis and migration of glioma cells was determined via cell-function assays. High SMYD2 expression correlated with a high WHO stage (P = 0.004) and a low survival probability (P = 0.012). The inhibition of SMYD2 suppressed the process of epithelial to mesenchymal transition (EMT) by downregulating the expression of Collagen 1A1 (COL1A1). AZ505 treatment significantly increased the drug sensitivity of glioma cells. SMYD2 expression was markedly reduced by cisplatin treatment via STIP1 Homology And U-Box Containing Protein 1 (STUB1)-mediated degradation. The knockdown of STUB1 could partly reverse the cell function impairment induced by cisplatin. Our findings suggested that SMYD2 could be a potential drug target for the treatment of gliomas, and STUB1-mediated degradation of SMYD2 plays an important role in reversing chemotherapy resistance in patients with gliomas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12031-022-02051-5DOI Listing
August 2022

High Prevalence and Persistence of Escherichia coli Strains Producing Shiga Toxin Subtype 2k in Goat Herds.

Microbiol Spectr 2022 Aug 8:e0157122. Epub 2022 Aug 8.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Shiga toxin (Stx)-producing Escherichia coli (STEC) is a zoonotic pathogen with the ability to cause severe diseases like hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Shiga toxin (Stx) is the key virulence factor in STEC and can be classified into two types, Stx1 and Stx2, and different subtypes. Stx2k is a newly reported Stx2 subtype in E. coli strains from diarrheal patients, animals, and raw meats exclusively in China so far. To understand the reservoir of Stx2k-producing E. coli (Stx2k-STEC), we investigated Stx2k-STEC strains in goat herds and examined their genetic characteristics using whole-genome sequencing. A total of 448 STEC strains were recovered from 2,896 goat fecal samples, and 37.95% (170/448) were Stx2k-STEC. Stx2k-STEC strains of serotype O93:H28 and sequence type 4038 (ST4038) were the most predominant and were detected over several years. Notably, 55% of Stx2k-STEC strains carried the heat-labile toxin (LT)-encoding gene () defining enterotoxigenic E. coli (ETEC), thereby exhibiting the hybrid STEC/ETEC pathotype. Stx2k-converting prophage genomes clustered into four groups and exhibited high similarity within each group. Strains from patients, raw meat, sheep, and goats were intermixed distributed in the phylogenetic tree, indicating the risk for cross-species spread of Stx2k-STEC and pathogenic potential for humans. Further studies are required to investigate the Stx2k-STEC strains in other reservoirs and to understand the mechanism of persistence in these hosts. Strains of the recently reported Stx2k-STEC have been circulating in a variety of sources over time in China. Here, we show a high prevalence of Stx2k-STEC in goat herds. More than half of the strains were of the hybrid STEC/ETEC pathotype. Stx2k-STEC strains of predominant serotypes have been widespread in the goat herds over several years. Stx2k-converting prophages have exhibited a high level of similarity across geographical regions and time and might be maintained and transmitted horizontally. Given that goat-derived Stx2k-STEC strains share similar genetic backbones with patient-derived strains, the high prevalence of Stx2k-STEC in goats suggests that there is a risk of cross-species spread and that these strains may pose pathogenetic potential to humans. Our study thus highlights the need to monitor human Stx2k-STEC infections in this region and, by extension, in other geographic locations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/spectrum.01571-22DOI Listing
August 2022

Age-Related Differences of Cortical Topology Across the Adult Lifespan: Evidence From a Multisite MRI Study With 1427 Individuals.

J Magn Reson Imaging 2022 Aug 3. Epub 2022 Aug 3.

Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Lanzhou, China.

Background: Healthy aging is usually accompanied by alterations in brain network architecture, influencing information processing and cognitive performance. However, age-associated coordination patterns of morphological networks and cognitive variation are not well understood.

Purpose: To investigate the age-related differences of cortical topology in morphological brain networks from multiple perspectives.

Study Type: Prospective, observational multisite study.

Population: A total of 1427 healthy participants (59.1% female, 51.75 ± 19.82 years old) from public datasets.

Field Strength/sequence: 1.5 T/3 T, T1-weighted magnetization prepared rapid gradient echo (MP-RAGE) sequence.

Assessment: The multimodal parcellation atlas was used to define regions of interest (ROIs). The Jensen-Shannon divergence-based individual morphological networks were constructed by estimating the interregional similarity of cortical thickness distribution. Graph-theory based global network properties were then calculated, followed by ROI analysis (including global/nodal topological analysis and hub analysis) with statistical tests.

Statistical Tests: Chi-square test, Jensen-Shannon divergence-based similarity measurement, general linear model with false discovery rate correction. Significance was set at P < 0.05.

Results: The clustering coefficient (q = 0.016), global efficiency (q = 0.007), and small-worldness (q = 0.006) were significantly negatively quadratic correlated with age. The group-level hubs of seven age groups were found mainly distributed in default mode network, visual network, salient network, and somatosensory motor network (the sum of these hubs' distribution in each group exceeds 55%). Further ROI-wise analysis showed significant nodal trajectories of intramodular connectivities.

Data Conclusion: These results demonstrated the age-associated reconfiguration of morphological networks. Specifically, network segregation/integration had an inverted U-shaped relationship with age, which indicated age-related differences in transmission efficiency.

Evidence Level: 2 TECHNICAL EFFICACY: Stage 1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmri.28318DOI Listing
August 2022

The Impact of Psychological Capital and Social Capital on Residents' Mental Health and Happiness During COVID-19: Evidence From China.

Front Psychol 2022 18;13:962373. Epub 2022 Jul 18.

Liberal Arts College, Changsha Normal University, Changsha, China.

Objective: This paper studies the mediating and interactive effects of social capital on psychological capital and the feeling of happiness from the impact of COVID-19. Since its emergence, the COVID-19 pandemic has taken a toll on people's mental health and affected their hopes for the future. Lifestyle and economic conditions have also been affected and have subsequently impacted people's sense of confidence in life. This could increase the likelihood of many people developing mental health issues, such as anxiety or depression. Therefore, it is vital to study the influence of psychological capital and social capital on people's subjective psychology and happiness experiences.

Materials And Methods: Using an ordered probit model, this paper studied the independent influence and interaction between psychological capital and social capital on people's happiness. The ordered probit model was chosen because subjective well-being (SWB) is an ordered variable. We further used structural equation modeling (SEM) to study the mediating effects of social capital on psychological capital and happiness.

Results: The regression results showed that both psychological capital and social capital were significantly positively correlated with happiness when controlling for other factors. In addition, psychological and social capital significantly interacted, in which the psychological capital promotes the effect of social capital on happiness. Moreover, the effect of psychological capital on happiness was greater than that of social capital, demonstrating that happiness is more greatly influenced by subjective psychological experience. The interaction coefficient of psychological and social capital was also significant, showing that the two have mutually reinforcing effects on happiness. Finally, health, income class, real estate, stranger trust, age, and urban household registration had significant positive effects on happiness, while the view of money, being female, education had a negative relationship with happiness. The SEM results showed that the mediating effect of psychological capital on happiness was partly transmitted through social capital: the total effect of psychological capital on happiness was highly significant ( < 0.0001), as was the total effect of social capital on happiness ( < 0.0001); however, the coefficient for psychological capital was greater than that for social capital. Through heterogeneity analysis, we found that the relationship between psychological capital, social capital, and happiness was significantly positive in each sub-sample group. There was also a significant interaction between psychological and social capital for men, women, urban and rural residents, and higher education background sample groups. However, the interaction was not significant in the sample group without higher education. In addition, the relationship between the happiness of rural residents and their educational background and gender was not significant.

Conclusion: We found that psychological and social capital have significant positive relationships and effects on happiness. Psychological capital demonstrated both direct and indirect influences on happiness, and further strengthens the influence of social capital on happiness. These results support a scheme to emphasize psychological support during the COVID-19 pandemic period to enhance the mental health of citizens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2022.962373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339779PMC
July 2022

Hybrid Ce-FeO/ZIF-67 Photoanode with Efficient Photoelectrochemical Water Oxidation Performance.

Inorg Chem 2022 Aug 3. Epub 2022 Aug 3.

Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.

Surface states and slow water oxidation kinetics greatly limit the photoelectrochemical (PEC) water oxidation performance of FeO. To solve the above problems, coupling FeO with a passivation layer and an oxygen evolution cocatalyst, respectively, is the common method. Though this method may improve its PEC performance, this also results in a low charge-transfer efficiency caused by the interface resistance between FeO and the modification materials (passivation layer and oxygen evolution cocatalyst). Therefore, it is important to identify a multifunctional modifier material to reduce the interfacial resistance due to the presence of multiple different materials. In this work, we introduced a thin cobalt-based metal-organic framework layer (ZIF-67) as a dual-functional material that acted as both a passivation layer and a water oxidation cocatalyst for a photoanode based on Ce-FeO nanorod arrays. The ZIF-67 layer inhibited charge carrier recombination by passivating the surface states. The PEC performance was improved due to the rich Co photogenerated hole-capture sites, which facilitated charge transfer and separation. As expected, the Ce-FeO/ZIF-67 photoanode exhibited superior water oxidation performance, with a photocurrent of 2.07 mA cm at 1.23 , which is 1.74 times higher than that of the Ce-FeO photoanode. The onset potential was negatively shifted by 71 mV. This study provides basic insights and a strategy for reducing interfacial resistance in hybrid materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.2c01491DOI Listing
August 2022

EEG based depression recognition using improved graph convolutional neural network.

Comput Biol Med 2022 Jul 8;148:105815. Epub 2022 Jul 8.

Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Lanzhou, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China; Joint Research Center for Cognitive Neurosensor Technology of Lanzhou University & Institute of Semiconductors, Chinese Academy of Sciences, China; Engineering Research Center of Open Source Software and Real-Time System (Lanzhou University), Ministry of Education, Lanzhou, China. Electronic address:

Depression is a global psychological disease that does serious harm to people. Traditional diagnostic method of the doctor-patient communication, is not objective and accurate enough. Thus, a more accurate and objective method for depression detection is urgently needed. Resting-state electroencephalography (EEG) can effectively reflect brain function, which have been used to study the difference of the brain between the depression patients and normal controls. In this work, the Resting-state EEG data of 27 depression patients and 28 normal controls was used in this study. We constructed the brain functional network using correlation, and extracted four linear features of EEG (activity, mobility complexity and power spectral density). We utilized a learnable weight matrix in the input layer of graph convolution neural network, creatively took the brain function network as the adjacency matrix input and the linear feature as the node feature input. We proposed our model Graph Input layer attention Convolutional Network (GICN), and it provided a good performance, showing the accuracy of 96.50% for recognition of depression and normal with 10-fold cross-validation, which indicated that our model could be used as an effective auxiliary tool for depression recognition. Besides, our method significantly outperformed other method. Additionally, the learnable weight matrix in the input layer was also used to find some edges and nodes that played an important role in depression recognition. Our findings showed that temporal lobe and parietal-occipital lobe had great effect in depression identification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiomed.2022.105815DOI Listing
July 2022

Prevalence of venous thromboembolism and evaluation of a modified caprini risk assessment model: a single-centre, prospective cohort study involving patients undergoing lung resections for bronchiectasis.

Thromb J 2022 Aug 1;20(1):43. Epub 2022 Aug 1.

Department of Thoracic Surgery, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Background: Venous thromboembolism (VTE) is a common postoperative complication in general thoracic surgery, but the incidence of patients undergoing surgery for bronchiectasis was not known. The purpose of our study was to investigate the incidence of VTE in bronchiectasis patients undergoing lung resection and to evaluate the risk stratification effect of the modified caprini risk assessment model (RAM).

Methods: We prospectively enrolled patients with bronchiectasis who underwent lung resection surgery between July 2016 and July 2020.The postoperative duplex lower-extremity ultrasonography or(and) computed tomographic pulmonary angiography (CTPA) was performed to detect VTE. The clinical characteristics and caprini scores of VTE patients and non-VTE patients would be compared and analyzed. Univariate logistic regression was performed to evaluate whether higher Caprini scores were associated with postoperative VTE risk.In addition, We explored the optimal cutoff for caprini score in patients with bronchiectasis by using the receiver operating characteristic (ROC) curve.

Results: One hundred and seventeen patients were eligible based on the prospective study criteria. The postoperative VTE incidence was 8.5% (10/117). By comparing the clinical characteristics and Caprini scores of VTE and non-VTE patients, the median preoperative hospitalization (7 vs 5 days, P = 0.028) and Caprini score (6.5 vs 3,P < 0.001) were significantly higher in VTE patients. In univariate logistic regression, a higher Caprini score was associated with higher odds ratio (OR) for VTE of 1.7, 95% confidence interval (CI) was from 1.2 to 2.5 (P = 0.001), C-statistics was 0.815 in the modified caprini RAM for predicting VTE. In a multivariable analysis adjusting for preoperative hospitalization, a higher Caprini score was associated with higher odds OR for VTE of 1.8 (95%CI: 1.2-2.6, P = 0.002), C-statistics was 0.893 in the caprini RAM for predicting VTE. When taking the Caprini score as 5 points as the diagnostic threshold, the Youden index is the largest.

Conclusions: The postoperative VTE incidence in patients undergoing lung resection for bronchiectasis was 8.5%.The modified caprini RAM effectively stratified bronchiectasis surgery patients for risk of VTE and showed excellent predictive power for VTE. The patients with postoperative caprini scores = 5, should be recommended to take positive measures to prevent postoperative VTE.

Trial Registration: Chinese Clinical Trial Register: ChiCTR-EOC-17010577.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12959-022-00402-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341115PMC
August 2022

Porous organic framework as coating for stir bar sorptive extraction of carbamate pesticides from corn and potato samples.

Food Chem 2022 Jul 26;397:133785. Epub 2022 Jul 26.

Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address:

Three porous organic frameworks (POFs) were synthesized by the reaction between phloroglucinol and 1,4-phthalaldehyde, 4,4'-biphenyldialdehyde or tris-(4-formylphenyl) amine; the products are named as POF-a, POF-b and POF-c, respectively. They were used to prepare POFs coated stir bars respectively for the extraction of four carbamate pesticides (CMPs). POF-c coated stir bar exhibited better adsorption performance than POF-a/b coated stir bar and commercial stir bars, probably due to the stronger conjugated structure and hydrophobicity of POF-c, and resultant hydrophobic, π-π and hydrogen bonding interactions between them. The adsorption mechanism for target CMPs was verified by characterization techniques and molecular dynamics simulation. A method of POF-c coated stir bar sorptive extraction-high performance liquid chromatography-variable wavelength ultraviolet detector was developed for the analysis of four CMPs in corn and potato samples. Under the optimal conditions, LODs of the method were between 0.017 and 0.048 μg/L, and the linear range for four CMPs was 0.1/0.2-200 μg/L.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.133785DOI Listing
July 2022

Deep learning-based defects detection of certain aero-engine blades and vanes with DDSC-YOLOv5s.

Sci Rep 2022 Jul 29;12(1):13067. Epub 2022 Jul 29.

College of Astronautics, Nanjing University of Aeronautics and Astronautics, Nanjing, 211156, China.

When performed by a person, aero-engine borescope inspection is easily influenced by individual experience and human factors that can lead to incorrect maintenance decisions, potentially resulting in serious disasters, as well as low efficiency. To address the absolute requirements of flight safety and improve efficiency to decrease maintenance costs, it is imperative to realize the intelligent detection of common aero-engine defects. YOLOv5 enables real-time detection of aero-engine defects with a high degree of accuracy. However, the performance of YOLOv5 is not optimal when detecting the same defects with multiple shapes. In this work, we introduce a deformable convolutional network into the structure of YOLOv5s to optimize its performance, overcome the disadvantage of the poor geometric transformability of convolutional neural networks, and enhance the adaptability of feature maps with large differences in the shape features. We also use a depth-wise separable convolution to improve the efficiency of multichannel convolution in extracting feature information from each channel at the same spatial position while reducing the increased computational effort due to the introduction of deformable convolution networks and use k-means clustering to optimize the size of anchor boxes. In the test results, mAP50 reached 83.8%. The detection accuracy of YOLOv5s for common aero-engine defects was effectively improved with only a 7.9% increase in calculation volume. Compared with the metrics of the original YOLOv5s, [email protected] was improved by 1.9%, and [email protected]:95 was improved by 1.2%. This study highlights the wide application potential of depth science methods in achieving intelligent detection of aero-engine defects. In addition, this study emphasizes the integration of DDSC-YOLOv5s into borescope platforms for scaled-up engine defect detection, which should also be enhanced in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-17340-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338258PMC
July 2022

Relationship between Metabolic Syndrome and Clinical Outcome in Patients Treated with Drug-Eluting Stenting after Rotational Atherectomy for Complex Calcified Coronary Lesions.

J Clin Med 2022 Jul 19;11(14). Epub 2022 Jul 19.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.

Background And Aims: although an association between metabolic syndrome (MS) and cardiovascular disease risk has been documented, the relationship in patients with complex calcified coronary lesions undergoing rotational atherectomy (RA) and drug-eluting stent(DES) insertion remains controversial. Here, the influence of MS on outcomes was assessed.

Methods And Results: we retrospectively included 398 patients who underwent RA and DES insertion for complex calcified coronary lesions in our institution between June 2015 and January 2019. The modified Adult Treatment Plan III was used to diagnose MS. The endpoint was major adverse cardiovascular events (MACEs), comprising mortality from all causes, myocardial infarction, and target vessel revascularization (TVR). In all, 173 (43.5%) patients had MS. MS was significantly associated with MACE over the 28.32 ± 6.79-month follow-up period (HR 1.783, 95% CI from 1.122 to 2.833) even after adjustment for other possible confounders.

Conclusion: MS was frequently observed in patients treated with RA with DES insertion for complex calcified coronary lesions. MS independently predicted MACE in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm11144192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322199PMC
July 2022

Lithium Chloride-Substituted Methylammonium Lead Tribromide Perovskites for Dual γ/Neutron Sensing.

ACS Appl Mater Interfaces 2022 Aug 22;14(30):34571-34582. Epub 2022 Jul 22.

Department of Nuclear Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.

Dual γ/neutron radiation sensors are a critical component of the nuclear security mission to prevent the proliferation of a special nuclear material (SNM). While high-performing semiconductors such as high purity germanium (HPGe) and CdZnTe (CZT) already exist in the nuclear security enterprise, their high cost and/or logistical burdens make widespread deployment difficult to achieve. Metal lead halide perovskites (MHPs) have attracted interest in recent years to address this challenge. In particular, methylammonium lead tribromide (CHNHPbBr, MAPbBr, or MAPB) has been widely evaluated for its radiation sensing capabilities. While previous studies have demonstrated low-energy X-ray and α particle sensing of MAPB-based detectors and several studies discuss the potential for γ ray sensing, neutron sensing of this material has been rarely explored. Here, we explore the incorporation of lithium in the form of LiCl into the MAPB structure to add thermal neutron sensitivity. Characterizations of the lithium-doped MAPB crystals demonstrate that quality growths are achievable with single crystals that exhibit high crystallinity, no phase change, and high macroscopic bulk quality. Finally, we report on the first demonstrated γ ray and thermal neutron sensing based on lithium-doped MAPB single crystals, which is a significant milestone in the development of 3D dual γ/neutron MHP sensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c05024DOI Listing
August 2022

Notch signaling leads to a slower progression of embryonic myogenic differentiation in Landrace than in Langtang pigs.

Acta Biochim Biophys Sin (Shanghai) 2022 Jul 25. Epub 2022 Jul 25.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China.

Delving into porcine embryonic myogenesis is the key to elucidate the complex regulation of breed-specific differences in growth performance and meat production. Increasing evidence proves that pigs with less meat production show earlier embryonic myogenesis, but little is known about the underlying mechanisms. In this study, we examine the longissimus dorsi muscle (LDM) by immunohistochemistry and confirm that the differentiation of myogenic progenitors is increased ( P <0.05) in Lantang (LT, fatty) pigs compared with that in Landrace (LR, lean) pigs, which results in more ( P <0.001) differentiated myoblasts (Pax7 /MyoD ) and less ( P <0.001) myogenic progenitors (Pax7 /MyoD ) in LT pigs at 35 days post-conception (35dpc). Additionally, embryonic myogenic progenitors isolated from LT pigs show greater ( P <0.001) differentiation capacity with earlier expression of MyoD compared with those from LR pigs. Moreover, Notch signaling is more active ( P <0.05) in LR pig myogenic progenitors than in LT pig myogenic progenitors. Inhibition of Notch signaling in LR myogenic progenitors suppresses Pax7 expression and increases MyoD expression, thus promoting myogenic differentiation. Consistently, the process of myogenic progenitors differentiating into myoblasts in ex vivo embryo limbs is accelerated when Notch signaling is inhibited. These results indicate that Notch signaling facilitates the maintenance of myogenic progenitors and antagonizes myogenic differentiation by promoting Pax7 expression and preventing MyoD expression in LR pigs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3724/abbs.2022095DOI Listing
July 2022

A novel classification and grading scale of palatine tonsil anatomy in children.

Eur Arch Otorhinolaryngol 2022 Jul 19. Epub 2022 Jul 19.

Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, No. 355, Luding Road, Shanghai, 200062, People's Republic of China.

Purpose: The influence of tonsil anatomical differences on post-tonsillectomy hemorrhage and pain has not been studied yet. This study aimed to establish a classification and grading scale of palatine tonsil anatomy, not size, for personalized post-tonsillectomy care.

Methods: Between August 2020 and August 2021, 337 children who underwent extracapsular tonsillectomy were recruited. The images of tonsil anatomy during the surgery were recorded and then classified and graded. Postoperative hemorrhage was recorded, and the degree of pain was measured using a visual analog scale (VAS). The primary outcomes were the associations between postoperative hemorrhage, pain, and the classification and grade of tonsil anatomy, analyzed by univariable and multivariable analyses.

Results: 186 of the 337 patients (55.2%) were male and 151 (44.8%) were female; the mean age was 5.59 years. The overall postoperative hemorrhage rate was 4.1%. The mean postoperative VAS score was 4.96. By univariable analysis with logistic regression model, significant associations were found between postoperative hemorrhage and the grade 2 and grade 3 tonsillar lower pole, and grade 3 tonsillar bed. Multivariable analysis with binary logistic regression model also revealed significant associations between postoperative hemorrhage and the grade 2 and grade 3 lower pole (OR: 8.23, 95% CI 1.01-67.37, P = 0.049; OR: 23.86, 95% CI 2.22-56.47, P = 0.009, respectively) and grade 3 tonsillar bed (OR: 14.25, 95% CI 1.46-18.75, P = 0.022). Linear regression analysis showed the associations between postoperative pain and grade 2 and grade 3 lower pole (β: 0.88, 95% CI 0.31-1.32, P = 0.002; β: 1.56, 95% CI 1.29-3.29, P = 0.001, respectively) and grade 3 anterior surface (β: 0.85, 95% CI 0.30-3.07, P = 0.004). Age and upper pole were not associated with the postoperative hemorrhage and pain neither.

Conclusion: In the present study, we established a novel classification and 3-grade scale of palatine tonsil anatomy, based on upper pole, anterior surface, lower pole, and tonsillar bed. Furthermore, we revealed for the first time that some anatomical characteristics of tonsils were associated with post-tonsillectomy complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00405-022-07515-3DOI Listing
July 2022

Construction of a novel rabbit model of ureteral calculi implanted with flowable resin.

BMC Urol 2022 Jul 18;22(1):105. Epub 2022 Jul 18.

Department of Urology, Guizhou Provincial People's Hospital, Guiyang, 550002, Guizhou Province, China.

Background: The purpose of this study was to characterize the pathophysiological changes of hydronephrosis caused by ureteral calculi obstruction in a new rabbit ureteral calculi model by implanting flowable resin.

Methods: Forty New Zealand rabbits were randomly divided into two groups: the calculi group and the sham control group. In the calculi group (n = 20), rabbits were operated at left lower abdomen and the left ureter was exposed. Then flowable resin (flowable restorative dental materials) was injected into the left ureter using a 0.45 mm diameter intravenous infusion needle. Then light-cured for 40 s by means of a dental curing light to form calculi. In the sham control group, normal saline was injected into the ureter. Rabbits underwent X-ray and routine blood and urine tests preoperatively, as well as X-ray, CT, dissection, HE staining and routine blood and urine tests on 1, 3, 5 and 7 days postoperatively. Stone formation was assessed by X-ray and unenhanced CT scan after surgery. The pathophysiological changes were evaluated through dissection, HE staining and routine blood and urine tests.

Results: Ureteral calculi models were successfully constructed in 17 rabbits. In calculi group, high-density shadows were observed in the left lower abdomen on postoperative day 1st, 3rd, 5th and 7th by X-ray and CT scan. Dissection found obstruction formation of the left ureters, dilatation of the renal pelvis and upper ureter during 7 days after surgery. The renal long-diameters of the left ureters increased only on the 1st postoperative day. HE staining found ureteral and kidney damage after surgery. In calculi group and sham group,the serum creatinine, urea nitrogen, white blood cells and urine red blood cells were raised at day 1 after surgery. However, the indicators returned to normal at day 3, 5, and 7.

Conclusions: This is a stable, less complicated operation and cost-effective ureteral calculi model by implanting flowable resin. And this novel model may allow us to further understand the pathophysiology changes caused by ureteral calculi obstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12894-022-01056-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295447PMC
July 2022

Brain Network Alterations in Rectal Cancer Survivors With Depression Tendency: Evaluation With Multimodal Magnetic Resonance Imaging.

Front Neurol 2022 29;13:791298. Epub 2022 Jun 29.

School of Information Science and Engineering, Lanzhou University, Lanzhou, China.

Surgery and chemotherapy may increase depression tendency in patients with rectal cancer (RC). Nevertheless, few comprehensive studies are conducted on alterations of brain network induced by depression tendency in patients with RC. Resting-state functional magnetic resonance imaging (rs-fMRI) and diffusion tensor imaging (DTI) data were collected from 42 patients with RC with surgery and chemotherapy and 38 healthy controls (HCs). Functional network (FN) was constructed from extracting average time courses in brain regions, and structural network (SN) was established by deterministic tractography. Graph theoretical analysis was used to calculate network properties. Networks resilient of two networks were assessed. Clinical correlation analysis was explored between altered network parameters and Hamilton depression scale (HAMD) score. This study revealed impaired FN and SN at both local and global levels and changed nodal efficiency and abnormal small-worldness property in patients with RC. On the whole, all FNs are more robust than SN. Moreover, compared with HC, patients with RC show less robustness in both networks. Regions with decreased nodal efficiency were associated with HAMD score. These cognitive dysfunctions are mainly attributable to depression-related brain functional and structural network alterations. Brain network reorganization is to prevent patients with RC from more serious depression after surgery and chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.791298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277124PMC
June 2022

Spin-related symmetry breaking induced by half-disordered hybridization in BiErRuO pyrochlores for acidic oxygen evolution.

Nat Commun 2022 Jul 15;13(1):4106. Epub 2022 Jul 15.

Jiangsu Key Laboratory for Nanotechnology and Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures, Nanjing University, Nanjing, 210093, People's Republic of China.

While acidic oxygen evolution reaction plays a critical role in electrochemical energy conversion devices, the sluggish reaction kinetics and poor stability in acidic electrolyte challenges materials development. Unlike traditional nano-structuring approaches, this work focuses on the structural symmetry breaking to rearrange spin electron occupation and optimize spin-dependent orbital interaction to alter charge transfer between catalysts and reactants. Herein, we propose an atomic half-disordering strategy in multistage-hybridized BiErRuO pyrochlores to reconfigure orbital degeneracy and spin-related electron occupation. This strategy involves controlling the bonding interaction of Bi-6s lone pair electrons, in which partial atom rearrangement makes the active sites transform into asymmetric high-spin states from symmetric low-spin states. As a result, the half-disordered BiErRuO pyrochlores demonstrate an overpotential of ~0.18 V at 10 mA cm accompanied with excellent stability of 100 h in acidic electrolyte. Our findings not only provide a strategy for designing atom-disorder-related catalysts, but also provides a deeper understanding of the spin-related acidic oxygen evolution reaction kinetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-31874-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287408PMC
July 2022

Data augmentation for depression detection using skeleton-based gait information.

Med Biol Eng Comput 2022 Sep 13;60(9):2665-2679. Epub 2022 Jul 13.

School of information Science and Engineering, Lanzhou University, Lanzhou, China.

In recent years, the incidence of depression is rising rapidly worldwide, but large-scale depression screening is still challenging. Gait analysis provides a non-contact, low-cost, and efficient early screening method for depression. However, the early screening of depression based on gait analysis lacks sufficient effective sample data. In this paper, we propose a skeleton data augmentation method for assessing the risk of depression. First, we propose five techniques to augment skeleton data and apply them to depression and emotion datasets. Then, we divide augmentation methods into two types (non-noise augmentation and noise augmentation) based on the mutual information and the classification accuracy. Finally, we explore which augmentation strategies can capture the characteristics of human skeleton data more effectively. Experimental results show that the augmented training dataset that retains more of the raw skeleton data properties determines the performance of the detection model. Specifically, rotation augmentation and channel mask augmentation make the depression detection accuracy reach 92.15% and 91.34%, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11517-022-02595-zDOI Listing
September 2022

Variants in Febrile Seizures/Epilepsy With Antecedent Febrile Seizures and Their Associations With Audio-Visual Abnormalities.

Front Mol Neurosci 2022 23;15:864074. Epub 2022 Jun 23.

Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Department of Neurology, Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Objective: gene encodes adhesion G protein-coupled receptor-V1 that is involved in synaptic function. mutations are associated with audio-visual disorders. Although previous experimental studies suggested that variants were associated with epilepsy, clinical evidence is limited and the phenotype spectrum is to be defined.

Methods: Trio-based targeting sequencing was performed in a cohort of 101 cases with febrile seizure (FS) and epilepsy with antecedent FS. Protein modeling was used to assess the damaging effects of variants. The genotype-phenotype correlations of the variants in epilepsy and audio-visual disorders were analyzed.

Results: variants were identified in nine unrelated cases (8.91%), including two heterozygous frameshift variants, six heterozygous missense variants, and a pair of compound heterozygous variants. These variants presented a statistically higher frequency in this cohort than that in control populations. Most missense variants were located at CalX-β motifs and changed the hydrogen bonds. These variants were inherited from the asymptomatic parents, indicating an incomplete penetrance. We also identified variants in 25 unrelated cases (24.75%) and variants in 3 unrelated cases (2.97%) in this cohort. Contrary to variant-associated epilepsy that revealed seizure was aggravated by sodium channel blockers, variants were associated with mild epilepsy with favorable responses to antiepileptic drugs. The patients denied problems with audio-visual-vestibular abilities in daily life. However, audio-visual tests revealed auditory and visual impairment in the patient with compound heterozygous variants, auditory or vestibular impairment in the patients with heterozygous frameshift, or hydrogen-bond changed missense variants but no abnormalities in the patients with missense variants without hydrogen-bond changes. Previously reported variants that were associated with audio-visual disorders were mostly biallelic/destructive variants, which were significantly more frequent in the severe phenotype of audio-visual disorders (Usher syndrome 2) than in other mild phenotypes. In contrast, the variants identified in epilepsy were monoallelic, missense mainly located at CalX-β, or affected isoforms VLGR1b/1c.

Significance: is potentially associated with FS-related epilepsy as a susceptibility gene. The genotype, submolecular implication, isoforms, and damaging severity of the variants explained the phenotypical variations. variant-associated FS/epilepsy presented favorable responses to antiepileptic drugs, implying a clinical significance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnmol.2022.864074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262510PMC
June 2022

Study on the Localization of Fangcang Shelter Hospitals During Pandemic Outbreaks.

Front Public Health 2022 21;10:876558. Epub 2022 Jun 21.

School of Public Health, Xuzhou Medical University, Xuzhou, China.

In the event of pandemic, it is essential for government authority to implement responses to control the pandemic and protect people's health with rapidity and efficicency. In this study, we first develop an evaluation framework consisting of the entropy weight method (EWM) and the technique for order preference by similarity to ideal solution (TOPSIS) to identify the preliminary selection of Fangcang shelter hospitals; next, we consider the timeliness of isolation and treatment of patients with different degrees of severity of the infectious disease, with the referral to and triage in Fangcang shelter hospitals characterized and two optimization models developed. The computational results of Model 1 and Model 2 are compared and analyzed. A case study in Xuzhou, Jiangsu Province, China, is used to demonstrate the real-life applicability of the proposed models. The two-stage localization method gives decision-makers more options in case of emergencies and can effectively designate the location. This article may give recommendations of and new insights into parameter settings in isolation hospital for governments and public health managers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2022.876558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253508PMC
June 2022

[Effects of Damming and Impoundment on Planktonic Microbial Community Structure and Interspecific Interaction Patterns in Different Water Depths].

Huan Jing Ke Xue 2022 Jul;43(7):3623-3634

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China.

Microbial communities play an important role in ecological processes through direct or indirect interactions. However, the interspecific interaction pattern of reservoir microorganisms has still received little attention. The Hengshan Reservoir in Jiangsu province was selected as the research area. Surface and deep water samples were collected to respectively explore the response of planktonic microbial community structure and the molecular ecological network to water depth changes, as well as to identify the key species of planktonic microbial communities in different water depths. The results showed that the richness and evenness of planktonic microorganisms in the surface layer were higher than those in the deep layer, and there were significant differences in the community structure of planktonic microorganisms in different depths. Water depth indirectly affected the planktonic microbial community by changing the environmental heterogeneity, among which the indicators such as TOC, NO, NH, and PO had the most significant impact on the planktonic microbial community structure. In addition, the number of nodes and edges of the deep planktonic microbial molecular ecological network were significantly higher than those on the surface, and the type and number of key species in the deep planktonic microbial community were significantly higher than those on the surface, indicating that the network scale of the deep planktonic microbial community was larger; however, the interspecific cooperative relationship and network connectivity of the surface planktonic microbial community were stronger. These results will provide a scientific basis for the study of interspecific interactions of reservoir microbial communities and are of great significance for predicting the ecological law of microorganisms in reservoir ecosystems and the impact of global material cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202109104DOI Listing
July 2022

Single Particle Inductively Coupled Plasma Mass Spectrometry-Based Homogeneous Detection of HBV DNA with Rolling Circle Amplification-Induced Gold Nanoparticle Agglomeration.

Anal Chem 2022 07 5;94(28):10011-10018. Epub 2022 Jul 5.

Department of Chemistry, Wuhan University, Wuhan 430072, China.

A highly sensitive and simple method based on rolling circle amplification (RCA) and single particle inductively coupled plasma mass spectrometry (spICP-MS) was proposed for the homogeneous detection of hepatitis B virus (HBV) deoxyribonucleic acid (DNA). In the presence of target DNA, long ssDNA possessing a large number of repeating sequence units was generated by RCA. DNA-labeled AuNP probes assembled into long chains based on complementary base pairing, further aggregating into large particles. Small Au NPs hardly produced pulse signals in spICP-MS; obvious pulse signals appeared in spICP-MS after the agglomeration of Au NPs caused by the addition of RCA products and spermidine. On the basis of this, the homogeneous detection of target DNA was realized by spICP-MS with high sensitivity. Under optimal conditions, the proposed method exhibited a good linear relationship between the frequency of the pulse signal of Au in spICP-MS and the concentration of target HBV DNA in the range of 10-2000 fmol L ( = 0.997), the limit of detection was 5.1 fmol L, and the relative standard deviation was 3.7-6.8%. Recoveries of 94.2-108% were obtained for target DNA in spiked serum samples, demonstrating a good matrix tolerance ability for the method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.2c00272DOI Listing
July 2022

Small Object Detection Pixel Level Balancing With Applications to Blood Cell Detection.

Front Physiol 2022 17;13:911297. Epub 2022 Jun 17.

Riseye Research, Riseye Intelligent Technology (Shanghai) Co., Ltd., Shanghai, China.

Object detection technology has been widely used in medical field, such as detecting the images of blood cell to count the changes and distribution for assisting the diagnosis of diseases. However, detecting small objects is one of the most challenging and important problems especially in medical scenarios. Most of the objects in medical images are very small but influential. Improving the detection performance of small objects is a very meaningful topic for medical detection. Current researches mainly focus on the extraction of small object features and data augmentation for small object samples, all of these researches focus on extracting the feature space of small objects better. However, in the training process of a detection model, objects of different sizes are mixed together, which may interfere with each other and affect the performance of small object detection. In this paper, we propose a method called pixel level balancing (PLB), which takes into account the number of pixels contained in the detection box as an impact factor to characterize the size of the inspected objects, and uses this as an impact factor. The training loss of each object of different size is adjusted by a weight dynamically, so as to improve the accuracy of small object detection. Finally, through experiments, we demonstrate that the size of objects in object detection interfere with each other. So that we can improve the accuracy of small object detection through PLB operation. This method can perform well with blood cell detection in our experiments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2022.911297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249342PMC
June 2022

Antifungal Mechanisms and Application of Lactic Acid Bacteria in Bakery Products: A Review.

Front Microbiol 2022 16;13:924398. Epub 2022 Jun 16.

College of Food Science, Sichuan Agricultural University, Ya'an, China.

Bakery products are nutritious, but they are susceptible to fungal contamination, which leads to a decline in quality and safety. Chemical preservatives are often used to extend the shelf-life of bakery products, but long-term consumption of these preservatives may increase the risk of chronic diseases. Consumers increasingly demand food with fewer chemical preservatives. The application of lactic acid bacteria (LAB) as a novel biological preservative not only prolongs the shelf-life of bakery products but also improves the baking properties of bakery products. This review summarizes different types and action mechanisms of antifungal compounds produced by LAB, factors affecting the production of antifungal compounds, and the effects of antifungal LAB on bakery products, providing a reference for future applications of antifungal LAB in bakery products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2022.924398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244174PMC
June 2022

The predictive value of Thromboelastography (TEG) parameters in vascular complications in patients with type 2 diabetes mellitus.

J Diabetes Complications 2022 Aug 24;36(8):108236. Epub 2022 Jun 24.

Department of Blood Transfusion, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo, Zhejiang, People's Republic of China.

Aims: The purpose of this research was to explore the association of TEG parameters (Reaction time, R; Clot kinetics, K; Alpha angle, α-angle; Maximum amplitude, MA) with vascular complications of type 2 diabetes mellitus (T2DM), and assess whether TEG parameters could predict T2DM patients with vascular complications.

Methods: A total of 68 healthy controls (HC), 57 T2DM patients without vascular complications (NC), 18 T2DM patients with only microvascular complications (MIC), 196 T2DM patients with only macrovascular complications (MAC), and 94 T2DM patients with both microvascular and macrovascular complications (MIC+MAC) were recruited in this study. Participants' clinical information and TEG parameters were recorded. TEG parameters were analyzed by the Jonckheere-Terpstra trend test, assuming the vascular complication was progressing from HC → NC → MIC → MIC+MAC or HC → NC → MAC → MIC+MAC. Receiver operating characteristic (ROC) was performed to explore the diagnostic accuracy of TEG parameters in T2DM with vascular complications.

Results: Shorter TEGK, higher TEG-α-angle, and higher TEG-MA were found in T2DM patients with both microvascular and macrovascular complications (MIC+MAC) group compared with healthy controls (HC) group and T2DM patients without vascular complications (NC) group (P < 0.05). Trend analysis showed that TEG-R/K decreased, but TEG-α-angle/MA increased gradually as the vascular complication progressed (P < 0.001). With stratification of urine microalbumin/creatinine ratio (UACR), diabetic nephropathy with macroalbuminuria (grade A3) behaves shorter TEGK, higher TEG-α-angle/MA compared with normal to mildly increased albuminuria (grade A1) and microalbuminuria (grade A2) (P < 0.05). ROC curves implied that TEGK, TEG-α-angle, and TEG-MA have moderate diagnostic values in T2DM without vascular complications (K-AUC: 0.780, α-angle-AUC: 0.773, and MA-AUC: 0.740) as well as T2DM with both microvascular and macrovascular complications (K-AUC: 0.778, α-angle-AUC: 0.757, and MA-AUC: 0.800).

Conclusion: TEG parameters are associated with the progression of vascular complications in T2DM, and it could be a diagnostic indicator for T2DM without vascular complications or with advanced vascular complications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdiacomp.2022.108236DOI Listing
August 2022

Noninvasive Diagnosis of Nasopharyngeal Carcinoma Based on Phenotypic Profiling of Viral and Tumor Markers on Plasma Extracellular Vesicles.

Anal Chem 2022 07 30;94(27):9740-9749. Epub 2022 Jun 30.

Department of Chemical Biology, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University, No. 422, Siming South Road, Xiamen 361005, Fujian, China.

Nasopharyngeal carcinoma (NPC) is a malignant tumor commonly associated with Epstein-Barr virus (EBV) infection, and its early diagnosis as well as its differentiation from nasopharyngitis (NPG) remains challenging due to the insufficient sensitivity of routine screening methods in clinical practice. To date, circulating extracellular vesicles (EVs, 40-1000 nm) have shown appealing potential in liquid biopsy for cancer diagnosis and prognosis. Herein, nanoflow cytometry (nFCM) capable of single EV analysis was applied to examine the expression of surface proteins with very low copy numbers on individual EVs as small as 40 nm. The particle concentrations of five EV subsets exposing EBV-encoded latent membrane proteins (LMP1 and LMP2A) and tumor markers (PD-L1, EGFR, and EpCAM) in plasma were determined rapidly via single-particle enumeration. We identified a five-marker panel named EV (an unweighted sum of the concentration of the five individual EV subsets) that significantly surpassed the traditional VCA-IgA assay in discriminating NPC patients from both healthy donors and NPG patients with accuracies of 96.3 and 83.1%, respectively. Moreover, EV (an unweighted sum of virus-specific LMP1- and LMP2A-positive EVs) could achieve the diagnosis of NPG with an accuracy of 82.6%. Collectively, the work presented a rapid, reliable, and noninvasive method as well as two diagnostic markers to help more accurately differentiate NPC from NPG patients and healthy donors in clinical practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.2c01311DOI Listing
July 2022

The genomic full-length sequence of the HLA-A*02:304 allele, identified by full-length group-specific sequencing.

HLA 2022 Jun 30. Epub 2022 Jun 30.

HLA Typing Laboratory, Institute of Transfusion Medicine, Qingdao Blood Center, Qingdao, Shandong Province, China.

The genomic full-length sequence of HLA-A*02:304 was identified by a group-specific sequencing approach from China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/tan.14728DOI Listing
June 2022

Effect of HIF-1α on estrogen/ERα signaling pathway in pituitary adenoma cells in hypoxic environment.

Authors:
Xin Wang Bin Hu

Minerva Surg 2022 Jun 28. Epub 2022 Jun 28.

Department of Endocrinology, Fuyang District Traditional Chinese Medicine Hospital, Hangzhou city, Hangzhou, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S2724-5691.21.09405-3DOI Listing
June 2022

Abnormal Brain Topological Structure of Mild Depression During Visual Search Processing Based on EEG Signals.

IEEE Trans Neural Syst Rehabil Eng 2022 4;30:1705-1715. Epub 2022 Jul 4.

Studies have shown that attention bias can affect behavioral indicators in patients with depression, but it is still unclear how this bias affects the brain network topology of patients with mild depression (MD). Therefore, a novel functional brain network analysis and hierarchical clustering methods were used to explore the abnormal brain topology of MD patients based on EEG signals during the visual search paradigm. The behavior results showed that the reaction time of MD group was significantly higher than that of normal group. The results of functional brain network indicated significant differences in functional connections between the two groups, the amount of inter-hemispheric long-distance connections are much larger than intra-hemispheric short-distance connections. Patients with MD showed significantly lower local efficiency and clustering coefficient, destroyed community structure of frontal lobe and parietal-occipital lobe, frontal asymmetry, especially in beta band. In addition, the average value of long-distance connections between left frontal and right parietal-occipital lobes presented significant correlation with depressive symptoms. Our results suggested that MD patients achieved long-distance connections between the frontal and parietal-occipital regions by sacrificing the connections within the regions, which might provide new insights into the abnormal cognitive processing mechanism of depression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNSRE.2022.3181690DOI Listing
July 2022

Perovskite-Carbon Joint Substrate for Practical Application in Proton Exchange Membrane Fuel Cells under Low-Humidity/High-Temperature Conditions.

ACS Appl Mater Interfaces 2022 Jul 27;14(27):30872-30880. Epub 2022 Jun 27.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing 211800, China.

Highly active catalysts with promising water retention are favorable for proton exchange membrane fuel cells (PEMFCs) operating under low-humidity/high-temperature conditions. When PEMFCs operate under low-humidity/high-temperature conditions, performance attenuation rapidly occurs owing to reduced proton conductivity of dehydrated membrane electrode assemblies. Herein, we load platinum onto a perovskite-carbon joint substrate (BaZrCeYYbO-XC-72R) to construct a highly active and durable catalyst with good water retention capacity. We propose that the Pt/(BZCYYb-C) catalyst layer at cathode can promote the water back diffusion of produced water from the cathode to the membrane, thus preventing the decay of fuel-cell performance under low-humidity/high-temperature conditions. The catalyst exhibited outstanding mass activity of 0.542 A mg at 0.9 V vs RHE. PEMFCs with such a catalyst delivered very high peak power densities (1.70/1.14 W cm under H-O/air conditions at 70 °C) and kept 85.3%/92.1% of initial performance values under low-humidity/high-temperature conditions (relative humidity 60%@70 °C/100 °C).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c06259DOI Listing
July 2022
-->