Publications by authors named "Bin Han"

560 Publications

Spatial distribution, source analysis, and ecological risk assessment of polycyclic aromatic hydrocarbons (PAHs) in the sediments from rivers emptying into Jiaozhou Bay, China.

Mar Pollut Bull 2021 Apr 30;168:112394. Epub 2021 Apr 30.

Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China; Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao 266071, China.

Sediment samples had been collected from five major rivers into Jiaozhou Bay, China to study the spatial distribution, sources, and ecological risks of 16 priority polycyclic aromatic hydrocarbons (PAHs) in April 2018. Results showed that the contents of PAHs in the sediments from the five rivers were significantly different. The distribution trend was Dagu < Yang < Licun < Baisha < Moshui River. The contents of PAHs in the eastern rivers were higher than those in the western portions. The contribution rate of HMW-PAHs was higher than LMW-PAHs in the sediments, with the majority of PAHs being four rings. PAHs in sediments were mainly derived from combustion sources, including the incomplete combustion of petroleum, coal, and wood. The benzo[a]pyrene toxic equivalent method (TEQ) showed that Moshui River had a high risk of carcinogenesis. The evaluation results of Effects Range Low(ERL), Effects Range Median (ERM) and Mean- Effects Range Median-quotient (M-ERM-Q) showed that PAHs in the sediments of Moshui River would occasionally have adverse biological effects, as well as possible medium and low ecological risks. The ecological risks of PAHs in the sediments of the other four rivers were low. Hence, we should focus on and control the input of PAHs in the Moshui River.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112394DOI Listing
April 2021

Two-Dimensional Covalent Organic Frameworks with Cobalt(II)-Phthalocyanine Sites for Efficient Electrocatalytic Carbon Dioxide Reduction.

J Am Chem Soc 2021 May 3. Epub 2021 May 3.

Beijing Advanced Innovation Center for Materials Genome Engineering, Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry and Chemical Engineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

The rapid development in synthesis methodology and applications for covalent organic frameworks (COFs) has been witnessed in recent years. However, the synthesis of highly stable functional COFs still remains a great challenge. Herein two-dimensional polyimide-linked phthalocyanine COFs (denoted as CoPc-PI-COF-1 and CoPc-PI-COF-2) have been devised and prepared through the solvothermal reaction of the tetraanhydrides of 2,3,9,10,16,17,23,24-octacarboxyphthalocyaninato cobalt(II) with 1,4-phenylenediamine and 4,4'-biphenyldiamine, respectively. The resultant CoPc-PI-COFs with a four-connected sql net exhibit AA stacking configurations according to powder X-ray diffraction studies, showing permanent porosity, thermal stability above 300 °C, and excellent resistance to a 12 M HCl aqueous solution for 20 days. Current-voltage curves reveal the conductivity of CoPc-PI-COF-1 and CoPc-PI-COF-2 with the value of 3.7 × 10 and 1.6 × 10 S m, respectively. Due to the same Co(II) electroactive sites together with similar permanent porosity and CO adsorption capacity for CoPc-PI-COFs, the cathodes made up of COFs and carbon black display a similar CO-to-CO Faradaic efficiency of 87-97% at applied potentials between -0.60 and -0.90 V (vs RHE) in 0.5 M KHCO solution. However, in comparison with the CoPc-PI-COF-2&carbon black electrode, the CoPc-PI-COF-1 counterpart provides a larger current density () of -21.2 mA cm at -0.90 V associated with its higher conductivity. This cathode also has a high turnover number and turnover frequency, amounting to 277 000 and 2.2 s at -0.70 V during 40 h of measurement. The present result clearly discloses the great potential of 2D porous crystalline solids in electrocatalysis.
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http://dx.doi.org/10.1021/jacs.1c02145DOI Listing
May 2021

Chromosome-scale assembly and analysis of biomass crop Miscanthus lutarioriparius genome.

Nat Commun 2021 04 28;12(1):2458. Epub 2021 Apr 28.

National Center for Gene Research, State Key Laboratory of Plant Molecular Genetics, CAS Center of Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200233, China.

Miscanthus, a rhizomatous perennial plant, has great potential for bioenergy production for its high biomass and stress tolerance. We report a chromosome-scale assembly of Miscanthus lutarioriparius genome by combining Oxford Nanopore sequencing and Hi-C technologies. The 2.07-Gb assembly covers 96.64% of the genome, with contig N50 of 1.71 Mb. The centromere and telomere sequences are assembled for all 19 chromosomes and chromosome 10, respectively. Allotetraploid origin of the M. lutarioriparius is confirmed using centromeric satellite repeats. The tetraploid genome structure and several chromosomal rearrangements relative to sorghum are clearly demonstrated. Tandem duplicate genes of M. lutarioriparius are functional enriched not only in terms related to stress response, but cell wall biosynthesis. Gene families related to disease resistance, cell wall biosynthesis and metal ion transport are greatly expanded and evolved. The expansion of these families may be an important genomic basis for the enhancement of remarkable traits of M. lutarioriparius.
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http://dx.doi.org/10.1038/s41467-021-22738-4DOI Listing
April 2021

MR to ultrasound image registration with segmentation-based learning for HDR prostate brachytherapy.

Med Phys 2021 Apr 27. Epub 2021 Apr 27.

Department of Radiation Oncology, Stanford University, Stanford, 94305, USA.

Purpose: Propagation of contours from high-quality magnetic resonance (MR) images to treatment planning ultrasound (US) images with severe needle artifacts is a challenging task, which can greatly aid the organ contouring in high dose rate (HDR) prostate brachytherapy. In this study, a deep learning approach was developed to automatize this registration procedure for HDR brachytherapy practice.

Methods: Because of the lack of training labels and difficulty of accurate registration from inferior image quality, a new segmentation-based registration framework was proposed for this multi-modality image registration problem. The framework consisted of two segmentation networks and a deformable registration network, based on the weakly-supervised registration strategy. Specifically, two 3D V-Nets were trained for the prostate segmentation on the MR and US images separately, to generate the weak supervision labels for the registration network training. Besides the image pair, the corresponding prostate probability maps from the segmentation were further fed to the registration network to predict the deformation matrix, and an augmentation method was designed to randomly scale the input and label probability maps during the registration network training. The overlap between the deformed and fixed prostate contours was analyzed to evaluate the registration accuracy. Three datasets were collected from our institution for the MR and US image segmentation networks, and the registration network learning, which contained 121, 104 and 63 patient cases, respectively.

Results: The mean Dice similarity coefficient (DSC) results of the two prostate segmentation networks are 0.86±0.05 and 0.90±0.03, for MR images and the US images after the needle insertion, respectively. The mean DSC, center-of-mass (COM) distance, Hausdorff distance (HD) and averaged symmetric surface distance (ASSD) results for the registration of manual prostate contours were 0.87±0.05, 1.70±0.89 mm, 7.21±2.07 mm, 1.61±0.64 mm, respectively. By providing the prostate probability map from the segmentation to the registration network, as well as applying the random map augmentation method, the evaluation results of the four metrics were all improved, such as an increase of DSC from 0.83±0.08 to 0.86±0.06 and from 0.86±0.06 to 0.87±0.05, respectively.

Conclusions: A novel segmentation-based registration framework was proposed to automatically register prostate MR images to the treatment planning US images with metal artifacts, which not only largely saved the labor work on the data preparation, but also improved the registration accuracy. The evaluation results showed the potential of this approach in HDR prostate brachytherapy practice.
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http://dx.doi.org/10.1002/mp.14901DOI Listing
April 2021

A rare case of difficult airway management in a Klippel-Feil syndrome pediatric patient with osseous torticollis undergone orthopedic surgery : Difficult airway in pediatric patient with torticollis.

BMC Anesthesiol 2021 Apr 19;21(1):121. Epub 2021 Apr 19.

Department of Anesthesiology, Peking University Third Hospital, 49 North Garden Rd., Haidian District, Beijing, China.

Background: Orthopedic surgery for cervical torticollis poses potential threat to airway management both in tracheal intubation and extubation. Klippel-Feil syndrome (KFS) is a complex syndrome of osseous and visceral anomalies. The anatomical characteristics of KFS might have significant implications for airway management.

Case Presentation: This is a rare case of an 8-year-old boy presenting with osseous torticollis, congenital occipito-atlantal deformity, congenital basilar invagination and KFS undergone elective torticollis correction surgery. Though with difficulty, tracheal intubation was successfully performed. Extubation failed twice on postoperative day 2 and 10, and required tracheostomy. Based on radiological findings, we speculated that prolonged airway edema accounted for the main reason of the failed extubation, the hypertrophic tonsil and occipito-cervical fusion resulted in reduced oropharyngeal space and limited cervical range of motion. Moreover, the Chiari malformation and KFS complicated the airway condition and lead to prolonged airway obstruction. The tracheostomy casing was removed 1 month later.

Conclusions: Cautions should be taken in extubation of pediatric patients undergone major osseous torticollis surgery. Reintubation should be prepared in case of failed extubation. Severe post-operative airway edema, complicated with hypertrophic tonsil, the structural abnormalities in the oropharyngeal cavity, and occipito-cervical deformities constituted the decreased oropharyngeal space and resulted in failed extubation. For severe airway compromise and prolonged intubation, tracheostomy should be considered.
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http://dx.doi.org/10.1186/s12871-021-01341-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054383PMC
April 2021

Perioperative Electroacupuncture Can Accelerate the Recovery of Gastrointestinal Function in Cancer Patients Undergoing Pancreatectomy or Gastrectomy: A Randomized Controlled Trial.

Evid Based Complement Alternat Med 2021 31;2021:5594263. Epub 2021 Mar 31.

State Key Lab of Molecular Oncology and Department of Pancreatic and Gastric Surgery, National Cancer Center, National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

The effect of perioperative acupuncture on accelerating gastrointestinal function recovery has been reported in colorectal surgery and distal gastrectomy (Billroth-II). However, the evidence in pancreatectomy and other gastrectomy is still limited. A prospective, randomized controlled trial was conducted between May 2018 and August 2019. Consecutive patients undergoing pancreatectomy or gastrectomy in our hospital were randomly assigned to the electroacupuncture (EA) group and the control group. The patients in the EA group received transcutaneous EA on Bai-hui (GV20), Nei-guan (PC6), Tian-shu (ST25), and Zu-san-li (ST36) once a day in the afternoon, and the control group received sham EA. Primary outcomes were the time to first flatus and time to first defecation. In total, 461 patients were randomly assigned to the groups, and 385 were analyzed finally (EA group,  = 201; control group,  = 184). Time to first flatus (3.0 ± 0.7 vs 4.2 ± 1.0, < 0.001) and first defecation (4.2 ± 0.9 vs 5.4 ± 1.2, < 0.001) in the EA group were significantly shorter than those in the control group. Of patients undergoing pancreatectomy, those undergoing pancreaticoduodenectomy and intraoperative radiation therapy (IORT) surgery benefitted from EA in time to first flatus ( < 0.001) and first defecation ( < 0.001), while those undergoing distal pancreatectomy did not (=0.157, =0.007) completely. Of patients undergoing gastrectomy, those undergoing total gastrectomy and distal gastrectomy (Billroth-II) benefitted from EA ( < 0.001), as did those undergoing proximal gastrectomy (=0.015). Patients undergoing distal gastrectomy (Billroth-I) benefitted from EA in time to first defecation (=0.012) but not flatus (=0.051). The time of parenteral nutrition, hospital stay, and time to first independent walk in the EA group were shorter than those in the control group. No severe EA complications were reported. EA was safe and effective in accelerating postoperative gastrointestinal function recovery. Patients undergoing pancreaticoduodenectomy, IORT surgery, total gastrectomy, proximal gastrectomy, or distal gastrectomy (Billroth-II) could benefit from EA. This trial is registered with NCT03291574.
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http://dx.doi.org/10.1155/2021/5594263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026294PMC
March 2021

Injectable kartogenin and apocynin loaded micelle enhances the alleviation of intervertebral disc degeneration by adipose-derived stem cell.

Bioact Mater 2021 Oct 23;6(10):3568-3579. Epub 2021 Mar 23.

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.

Cell transplantation has been proved the promising therapeutic effects on intervertebral disc degeneration (IVDD). However, the increased levels of reactive oxygen species (ROS) in the degenerated region will impede the efficiency of human adipose-derived stem cells (human ADSCs) transplantation therapy. It inhibits human ADSCs proliferation, and increases human ADSCs apoptosis. Herein, we firstly devised a novel amphiphilic copolymer PEG-PAPO, which could self-assemble into a nanosized micelle and load lipophilic kartogenin (KGN), as a single complex (PAKM). It was an injectable esterase-responsive micelle, and showed controlled release ability of KGN and apocynin (APO). Oxidative stimulation promoted the esterase activity in human ADSCs, which accelerate degradation of esterase-responsive micelle. Compared its monomer, the PAKM micelle possessed better bioactivities, which were attributed to their synergistic effect. It enhanced the viability, autophagic activation (P62, LC3 II), ECM-related transcription factor (SOX9), and ECM (Collagen II, Aggrecan) maintenance in human ADSCs. Furthermore, it is demonstrated that the injection of PAKM with human ADSCs yielded higher disc height and water content in rats. Therefore, PAKM micelles perform promoting cell survival and differentiation effects, and may be a potential therapeutic agent for IVDD.
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http://dx.doi.org/10.1016/j.bioactmat.2021.03.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022109PMC
October 2021

Maternal exposure to ambient air pollution and congenital heart defects in China.

Environ Int 2021 Apr 7;153:106548. Epub 2021 Apr 7.

Guangdong Provincial Engineering Technology Research Center of Environmental and Health Risk Assessment, Department of Occupational and Environmental Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China. Electronic address:

Background: Evidence of maternal exposure to ambient air pollution on congenital heart defects (CHD) has been mixed and are still relatively limited in developing countries. We aimed to investigate the association between maternal exposure to air pollution and CHD in China.

Method: This longitudinal, population-based, case-control study consecutively recruited fetuses with CHD and healthy volunteers from 21 cities, Southern China, between January 2006 and December 2016. Residential address at delivery was linked to random forests models to estimate maternal exposure to particulate matter with an aerodynamic diameter of ≤ 1 µm (PM), ≤2.5 µm, and ≤10 µm as well as nitrogen dioxides, in three trimesters. The CHD cases were evaluated by obstetrician, pediatrician, or cardiologist, and confirmed by cardia ultrasound. The CHD subtypes were coded using the International Classification Diseases. Adjusted logistic regression models were used to assess the associations between air pollutants and CHD and its subtypes.

Results: A total of 7055 isolated CHD and 6423 controls were included in the current analysis. Maternal air pollution exposures were consistently higher among cases than those among controls. Logistic regression analyses showed that maternal exposure to all air pollutants during the first trimester was associated with an increased odds of CHD (e.g., an interquartile range [13.3 µg/m] increase in PM was associated with 1.09-fold ([95% confidence interval, 1.01-1.18]) greater odds of CHD). No significant associations were observed for maternal air pollution exposures during the second trimester and the third trimester. The pattern of the associations between air pollutants and different CHD subtypes was mixed.

Conclusions: Maternal exposure to greater levels of air pollutants during the pregnancy, especially the first trimester, is associated with higher odds of CHD in offspring. Further longitudinal well-designed studies are warranted to confirm our findings.
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http://dx.doi.org/10.1016/j.envint.2021.106548DOI Listing
April 2021

A primary human hepatocyte/hepatic stellate cell co-culture system for improved in vitro HBV replication.

Virology 2021 Mar 26;559:40-45. Epub 2021 Mar 26.

Gilead Sciences, Inc., Foster City, CA, USA. Electronic address:

Primary human hepatocytes (PHHs) are considered the gold standard for the in vitro study of HBV replication as they directly reflect the metabolism and functionality of the human liver. However, several limitations of this system include PHH donor-to-donor variability, limited life span and low permissiveness to HBV infection, which precludes long-term infection studies and viral passaging. Here, an easy-to-set-up co-culture platform that combines PHH with hepatic stellate cells (HSCs) was developed. This platform does not rely on chemical supplementation to sustain robust HBV replication and viral antigen secretion making it a more physiologically relevant system for in vitro HBV infection studies compared to the traditional short-lived PHH monocultures.
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http://dx.doi.org/10.1016/j.virol.2021.03.012DOI Listing
March 2021

Microglial PGC-1α protects against ischemic brain injury by suppressing neuroinflammation.

Genome Med 2021 Mar 26;13(1):47. Epub 2021 Mar 26.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.

Background: Neuroinflammation and immune responses occurring minutes to hours after stroke are associated with brain injury after acute ischemic stroke (AIS). PPARγ coactivator-1α (PGC-1α), as a master coregulator of gene expression in mitochondrial biogenesis, was found to be transiently upregulated in microglia after AIS. However, the role of microglial PGC-1α in poststroke immune modulation remains unknown.

Methods: PGC-1α expression in microglia from human and mouse brain samples following ischemic stroke was first determined. Subsequently, we employed transgenic mice with microglia-specific overexpression of PGC-1α for middle cerebral artery occlusion (MCAO). The morphology and gene expression profile of microglia with PGC-1α overexpression were evaluated. Downstream inflammatory cytokine production and NLRP3 activation were also determined. ChIP-Seq analysis was performed to detect PGC-1α-binding sites in microglia. Autophagic and mitophagic activity was further monitored by immunofluorescence staining. Unc-51-like autophagy activating kinase 1 (ULK1) expression was evaluated under the PGC-1α interaction with ERRα. Finally, pharmacological inhibition and genomic knockdown of ULK1 were performed to estimate the role of ULK1 in mediating mitophagic activity after ischemic stroke.

Results: PGC-1α expression was shortly increased after ischemic stroke, not only in human brain samples but also in mouse brain samples. Microglia-specific PGC-1α overexpressing mice exhibited significantly decreased neurologic deficits after ischemic injury, with reduced NLRP3 activation and proinflammatory cytokine production. ChIP-Seq analysis and KEGG pathway analysis revealed that mitophagy was significantly enhanced. PGC-1α significantly promoted autophagic flux and induced autolysosome formation. More specifically, the autophagic clearance of mitochondria was enhanced by PGC-1α regulation, indicating the important role of mitophagy. Pharmacological inhibition or knockdown of ULK1 expression impaired autophagic/mitophagic activity, thus abolishing the neuroprotective effects of PGC-1α.

Conclusions: Mechanistically, in AIS, PGC-1α promotes autophagy and mitophagy through ULK1 and reduces NLRP3 activation. Our findings indicate that microglial PGC-1α may be a promising therapeutic target for AIS.
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http://dx.doi.org/10.1186/s13073-021-00863-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004413PMC
March 2021

Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers.

Elife 2021 03 24;10. Epub 2021 Mar 24.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Science, Beijing, China.

Behavioral specialization is key to the success of social insects and leads to division of labor among colony members. Response thresholds to task-specific stimuli are thought to proximally regulate behavioral specialization, but their neurobiological regulation is complex and not well understood. Here, we show that response thresholds to task-relevant stimuli correspond to the specialization of three behavioral phenotypes of honeybee workers in the well-studied and important and . Quantitative neuropeptidome comparisons suggest two tachykinin-related peptides (TRP2 and TRP3) as candidates for the modification of these response thresholds. Based on our characterization of their receptor binding and downstream signaling, we confirm a functional role of tachykinin signaling in regulating specific responsiveness of honeybee workers: TRP2 injection and RNAi-mediated downregulation cause consistent, opposite effects on responsiveness to task-specific stimuli of each behaviorally specialized phenotype but not to stimuli that are unrelated to their tasks. Thus, our study demonstrates that TRP signaling regulates the degree of task-specific responsiveness of specialized honeybee workers and may control the context specificity of behavior in animals more generally.
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http://dx.doi.org/10.7554/eLife.64830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016481PMC
March 2021

Enhancing rice grain production by manipulating the naturally evolved cis-regulatory element-containing inverted repeat sequence of OsREM20.

Mol Plant 2021 Mar 16. Epub 2021 Mar 16.

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address:

Grain number per panicle (GNP) is an important agronomic trait that contributes to rice grain yield. Despite its importance in rice breeding, the molecular mechanism underlying GNP regulation remains largely unknown. In this study, we identified a previously unrecognized regulatory gene that controls GNP in rice, Oryza sativa REPRODUCTIVE MERISTEM 20 (OsREM20), which encodes a B3 domain transcription factor. Through genetic analysis and transgenic validation we found that genetic variation in the CArG box-containing inverted repeat (IR) sequence of the OsREM20 promoter alters its expression level and contributes to GNP variation among rice varieties. Furthermore, we revealed that the IR sequence regulates OsREM20 expression by affecting the direct binding of OsMADS34 to the CArG box within the IR sequence. Interestingly, the divergent pOsREM20 and pOsREM20 alleles were found to originate from different Oryza rufipogon accessions, and were independently inherited into the japonica and indica subspecies, respectively, during domestication. Importantly, we demonstrated that IR sequence variations in the OsREM20 promoter can be utilized for germplasm improvement through either genome editing or traditional breeding. Taken together, our study characterizes novel genetic variations responsible for GNP diversity in rice, reveals the underlying molecular mechanism in the regulation of agronomically important gene expression, and provides a promising strategy for improving rice production by manipulating the cis-regulatory element-containing IR sequence.
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http://dx.doi.org/10.1016/j.molp.2021.03.016DOI Listing
March 2021

The prevalence and risk factors for depressive symptoms in frontline nurses under COVID-19 pandemic based on a large cross-sectional study using the propensity score-matched method.

BMC Psychiatry 2021 03 16;21(1):152. Epub 2021 Mar 16.

Department of Urology, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Shenyang, 110004, Liaoning, China.

Introduction: To explore the prevalence of depressive symptoms and the associated risk factors in frontline nurses under COVID-19 pandemic.

Methods: This cross-sectional study was conducted from February 20, 2020 to March 20, 2020 and involved 562 frontline nurses. The effective response rate was 87.68%. After propensity score matched, there were 498 participants left. Extensive characteristics, including demographics, dietary habits, life-related factors, work-related factors, and psychological factors were collected based on a self-reported questionnaire. Specific scales measured the levels of sleep quality, physical activity, depressive symptoms, perceived organization support and psychological capital. Adjusted odds ratios and 95% confidence intervals were determined by binary paired logistic regression.

Results: Of the nurses enrolled in the study, 50.90% had depressive symptoms. Three independent risk factors were identified: poor sleep quality (OR = 1.608, 95% CI: 1.384-1.896), lower optimism of psychological capital (OR = 0.879, 95% CI: 0.805-0.960) and no visiting friend constantly (OR = 0.513, 95% CI: 0.286-0.920).

Conclusions: This study revealed a considerable high prevalence of depressive symptoms in frontline nurses during the COVID-19 outbreak, and identified three risk factors, which were poor sleep quality, lower optimism of psychological capital, and no visiting friend constantly. Protecting mental health of nurses is important for COVID-19 pandemic control and their wellbeing. These findings enrich the existing theoretical model of depression and demonstrated a critical need for additional strategies that could address the mental health in frontline nurses for policymakers.
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http://dx.doi.org/10.1186/s12888-021-03143-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961324PMC
March 2021

OryzaGenome2.1: Database of Diverse Genotypes in Wild Oryza Species.

Rice (N Y) 2021 Mar 4;14(1):24. Epub 2021 Mar 4.

National Institute of Genetics, Yata 1111, Mishima, Shizuoka, 411-8540, Japan.

Background: OryzaGenome ( http://viewer.shigen.info/oryzagenome21detail/index.xhtml ), a feature within Oryzabase ( https://shigen.nig.ac.jp/rice/oryzabase/ ), is a genomic database for wild Oryza species that provides comparative and evolutionary genomics approaches for the rice research community.

Results: Here we release OryzaGenome2.1, the first major update of OryzaGenome. The main feature in this version is the inclusion of newly sequenced genotypes and their meta-information, giving a total of 217 accessions of 19 wild Oryza species (O. rufipogon, O. barthii, O. longistaminata, O. meridionalis, O. glumaepatula, O. punctata, O. minuta, O. officinalis, O. rhizomatis, O. eichingeri, O. latifolia, O. alta, O. grandiglumis, O. australiensis, O. brachyantha, O. granulata, O. meyeriana, O. ridleyi, and O. longiglumis). These 19 wild species belong to 9 genome types (AA, BB, CC, BBCC, CCDD, EE, FF, GG, and HHJJ), representing wide genomic diversity in the genus. Using the genotype information, we analyzed the genome diversity of Oryza species. Other features of OryzaGenome facilitate the use of information on single nucleotide polymorphisms (SNPs) between O. sativa and its wild progenitor O. rufipogon in rice research, including breeding as well as basic science. For example, we provide Variant Call Format (VCF) files for genome-wide SNPs of 33 O. rufipogon accessions against the O. sativa reference genome, IRGSP1.0. In addition, we provide a new SNP Effect Table function, allowing users to identify SNPs or small insertion/deletion polymorphisms in the 33 O. rufipogon accessions and to search for the effect of these polymorphisms on protein function if they reside in the coding region (e.g., are missense or nonsense mutations). Furthermore, the SNP Viewer for 446 O. rufipogon accessions was updated by implementing new tracks for possible selective sweep regions and highly mutated regions that were potentially exposed to selective pressures during the process of domestication.

Conclusion: OryzaGenome2.1 focuses on comparative genomic analysis of diverse wild Oryza accessions collected around the world and on the development of resources to speed up the identification of critical trait-related genes, especially from O. rufipogon. It aims to promote the use of genotype information from wild accessions in rice breeding and potential future crop improvements. Diverse genotypes will be a key resource for evolutionary studies in Oryza, including polyploid biology.
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http://dx.doi.org/10.1186/s12284-021-00468-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933306PMC
March 2021

Simultaneous fluorescence determination of bisphenol A and its halogenated analogs based on a molecularly imprinted paper-based analytical device and a segment detection strategy.

Biosens Bioelectron 2021 May 20;180:113106. Epub 2021 Feb 20.

State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei, 430030, China. Electronic address:

Bisphenol A (BPA) and its halogenated analogs tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) are common environmental contaminants and a method for their simultaneous determination is urgently needed. A paper-based analytical device (PAD) was prepared using a metal-organic framework of UiO-66-NH coated with molecularly imprinted polymers (MIPs) using TBBPA as a template. The maximum adsorption capacity was 120.94 mg g and the imprinting factor was 4.07. The selective recognition ability of this PAD enabled the effective separation of TBBPA, TCBPA and BPA based on paper chromatography. Subsequently, the PAD cut into segments were used individually to determine the presence of target chemicals using a highly sensitive fluorescent method. Under ultraviolet light irradiation, UiO-66-NH acts as a photocatalyst to produce reactive oxygen species (ROS) that degrade TBBPA, TCBPA or BPA in the imprinted cavities and the fluorescent signal of 2',7'-dichlorodihydrofluorescein diacetate (HDCFDA) added as a ROS probe enabled the indirect determination of target chemicals. This method could determine BPA and its halogenated analogs in dust samples simultaneously with detection limits ranging from 0.14 to 0.30 ng g. The intraday relative standard deviation (RSD) was ≤6.8% and interday RSD was ≤8.1%. The recoveries ranged from 91.0 to 105.6% with RSD values that were ≤7.5%. The results stemmed from this method were consistent with those obtained from LC-MS/MS. It is an environmentally-friendly approach due to the degradation of target pollutants and possesses many advantages such as high selectivity, low cost and easy-to-fabrication.
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http://dx.doi.org/10.1016/j.bios.2021.113106DOI Listing
May 2021

Therapeutic potential of puerarin against methionine-choline-deficient diet-induced non-alcoholic steatohepatitis determined by combination of H NMR spectroscopy-based metabonomics and 16S rRNA gene sequencing.

J Pharm Biomed Anal 2021 Apr 9;197:113964. Epub 2021 Feb 9.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address:

Previously published studies have revealed the protective effect of puerarin against non-alcoholic steatohepatitis (NASH), but the definite mechanism of this effect still remains unclear. The present work was an attempt to assess the beneficial effects and the underlying mechanisms of puerarin on methionine-choline-deficient (MCD) diet-induced NASH in C57BL/6 mice by using a combination of metabonomics and 16S rRNA gene sequencing technology. Nuclear magnetic resonance (NMR)-based metabonomics showed significant hepatic and urinary metabolic phenotype changes between MCD-diet fed mice and the healthy controls. A total of eight and thirteen metabolites were identified as differential metabolites associated with NASH in liver tissue and urine of mice, respectively. The proposed pathways mainly included pyrimidine metabolism, one-carbon metabolism, amino acid metabolism, glycolysis, tricarboxylic acid (TCA) cycle and synthesis and degradation of ketone bodies. Furthermore, 16S rRNA gene sequencing analysis delineated remarkable variations in gut microbiota profiles in response to MCD diet in mice and forty differential bacterial taxa related to NASH were found between the control and model group. Puerarin could improve hepatic steatosis and inflammation in NASH mice via partially ameliorating metabolic disorders and rebalancing the gut flora. Specifically, puerarin could inhibit lipopolysaccharide (LPS)-producing genus Helicobacter, and promote butyrate-producing genus Roseburia. These findings offered novel insights into the in-depth understanding of the pathogenesis of NASH and provided further evidence for the potential use of puerarin as an anti-NASH agent.
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http://dx.doi.org/10.1016/j.jpba.2021.113964DOI Listing
April 2021

Aircraft-based observation of gaseous pollutants in the lower troposphere over the Beijing-Tianjin-Hebei region.

Sci Total Environ 2021 Jun 27;773:144818. Epub 2021 Jan 27.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China.

To investigate the spatial and vertical distribution of atmospheric pollutants (SO, NO, CO and O), aircraft-based measurements (model: Yun-12, 12 flights, 27 h total flight time) were conducted from near the surface up to 2400 m over the Beijing-Tianjin-Hebei (BTH) region between June 17th and July 22nd 2016. The results showed that high concentrations of primary gaseous pollutants (SO, NO, CO) were generally present in Beijing, Tianjin, Langfang and Tangshan areas, while high values of O frequently appeared in areas far from the city. The flights at noon and dusk measured higher O concentrations at 600 m and lower O concentrations at higher altitudes, implying a strong influence by photochemical production. Back trajectory analysis suggested that the high levels of gaseous pollutants, especially at 600 m, were associated with pollution sources transported from the southerly direction during the observation period. The first simultaneous vertical distribution measurements using aircraft and tethered balloon were conducted in Gaocun (a rural site between Beijing and Tianjin) on June 17th. The results indicated that an inversion layer at the top of the planetary boundary layer (PBL) significantly suppressed vertical exchange through the PBL and resulted in a "two-layer" vertical distribution of pollutants above and below the PBL. Additionally, a residual high O layer (79.9 ± 2.5 ppb, 500-1000 m) was observed above the PBL, and it contributed to the surface peak O level at noon through downward transport along with the opening up of the PBL. These results indicate that coupled effects of horizontal and vertical transport should be investigated in future studies to improve the chemical transport models used to study the vertical distribution and regional transport over the BTH region.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144818DOI Listing
June 2021

Impact of the Perioperative Blood Pressure on Clinical Outcome after Thrombectomy in Acute Basilar Artery Occlusion.

J Stroke Cerebrovasc Dis 2021 Mar 9;30(3):105590. Epub 2021 Jan 9.

Neurointervention Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China. Electronic address:

Aim: Optimal blood pressure (BP) management in vertebrobasilar circulation stroke patients undergoing thrombectomy remains undetermined. We aimed to evaluate the impact of perioperative BP on clinical outcome after MT in acute basilar artery occlusion (BAO) patients.

Methods: We retrospectively analyzed all consecutive patients hospitalized with acute basilar artery occlusion administered endovascular treatment within 24 h from January 2012 to July 2018 in Beijing Tiantan Hospital. BP was measured at regular intervals during the first 24 h after stroke onset, during and after thrombectomy. The clinical outcomes assessed at 3-month follow up were functional independence (mRS score of 0-2) and mortality (mRS score of 6).

Results: Of the 187 treated patients, 157 were male; patient ages were 60±10 years. The median NIHSS on admission was 22. Totally in 179 patients had complete BP level assessment. In these individuals, univariate analysis revealed significant associations of postoperative Max SBP and Max MAP with mortality (all P < 0.05). Multivariate regression analysis also demonstrated that postoperative Max SBP (OR=0.964, 95% CI 0.941 to 0.987, P < 0.003) and Max MAP (OR=0.942, 95% CI 0.907 to 0.979, P < 0.002) were independent predictors of mortality.

Conclusions: In acute BAO patients administered thrombectomy, Max SBP between 120 and 160 mmHg may be associated with better outcome, with a trend of reduced risk of mortality.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105590DOI Listing
March 2021

A rare case of an unexpected difficult airway management in a diffuse idiopathic skeletal hyperostosis patient and post-operative airway evaluation with 3D printing technique.

Ann Transl Med 2021 Jan;9(1):75

Department of Anesthesiology, Peking University Third Hospital, Beijing, China.

Diffuse idiopathic skeletal hyperostosis (DISH), asymptomatic in most cases, is a degenerative condition that commonly leads to anterior cervical osteophytes in most elderly patients. Clinically significant airway compression is rare. However, in some cases, the seemingly insignificant osteophytes could become a threat to airway management during intubation. Here we present a case of an 82-year-old man diagnosed as DISH and scheduled for cervical surgery. Radiographic imaging did not indicate the airway compromise. Preoperative airway assessment indicated modified Mallampati scoring of class III. However, an unexpected large bulge protruding from posterior pharyngeal wall blocked video laryngoscopy assisted intubation. Intubation with direct laryngoscope, laryngeal mask airway (LMAD) and Shikani optical stylet were failed. Only flexible fiberoptic nasal intubation was performed successfully. After surgery, an anatomically matched and patient-specific 3D printed model was made to help more direct and comprehensive estimation of the size and the location of the osteophyte. The relationship of the airway compromise and osteophytes was revealed in DISH patients for the first time with the aid of 3D printed model. The eccentric growth of the cervical osteophytes occupied large portion of the laryngopharynx space and prohibited the direct placement of the tracheal tube. This case is a rare presentation of the management of a DISH patient with unexpected difficult airway. We propose for the first time that different intubation strategies should be considered for potential difficulty airway in DISH patients according to the size and location of the osteophytes. For potential difficult airway management of DISH patients, 3D printing technique is a promising way of preoperative airway assessment.
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http://dx.doi.org/10.21037/atm-20-5992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859815PMC
January 2021

A route to de novo domestication of wild allotetraploid rice.

Cell 2021 Mar 3;184(5):1156-1170.e14. Epub 2021 Feb 3.

State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address:

Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired because of its advantages in genome buffering, vigorousness, and environmental robustness. However, a workable route remains elusive. Here, we describe a practical strategy, namely de novo domestication of wild allotetraploid rice. By screening allotetraploid wild rice inventory, we identified one genotype of Oryza alta (CCDD), polyploid rice 1 (PPR1), and established two important resources for its de novo domestication: (1) an efficient tissue culture, transformation, and genome editing system and (2) a high-quality genome assembly discriminated into two subgenomes of 12 chromosomes apiece. With these resources, we show that six agronomically important traits could be rapidly improved by editing O. alta homologs of the genes controlling these traits in diploid rice. Our results demonstrate the possibility that de novo domesticated allotetraploid rice can be developed into a new staple cereal to strengthen world food security.
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http://dx.doi.org/10.1016/j.cell.2021.01.013DOI Listing
March 2021

A quantitative genomics map of rice provides genetic insights and guides breeding.

Nat Genet 2021 02 1;53(2):243-253. Epub 2021 Feb 1.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.

Extensive allelic variation in agronomically important genes serves as the basis of rice breeding. Here, we present a comprehensive map of rice quantitative trait nucleotides (QTNs) and inferred QTN effects based on eight genome-wide association study cohorts. Population genetic analyses revealed that domestication, local adaptation and heterosis are all associated with QTN allele frequency changes. A genome navigation system, RiceNavi, was developed for QTN pyramiding and breeding route optimization, and implemented in the improvement of a widely cultivated indica variety. This work presents an efficient platform that bridges ever-increasing genomic knowledge and diverse improvement needs in rice.
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http://dx.doi.org/10.1038/s41588-020-00769-9DOI Listing
February 2021

Metabolic profiling unravels the effects of enhanced output and harvesting time on royal jelly quality.

Food Res Int 2021 Jan 13;139:109974. Epub 2020 Dec 13.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China. Electronic address:

Royal jelly (RJ), a natural secretion of nurse bees, is a functional food with health-promoting properties. To investigate RJ quality changes with enhanced output and harvesting time, we compared metabolic profiles of RJ harvested at 24 h, 48 h, and 72 h after larval grafting into queen cells from Italian bees (ITBs) and high RJ-producing bees (RJBs) selected from ITBs. A total of 77 high-abundance compounds were identified with reverse-phase liquid chromatography- and hydrophilic interaction liquid chromatography-high-resolution mass spectrometry. Metabolite abundance, water content, and antibacterial activity were found largely unchanged between both bee strains, indicating that a similar RJ quality was achieved. Notably, the observed 10-hydroxy-2-decenoic acid content in RJBs (1.98%) and ITBs (2.41%) meet the standard for premium RJ in China. RJ harvested at 48 h and 72 h had similar metabolic profiles, indicating little influence of the two harvesting times on RJ quality. The harvesting at 24 h could lead to a higher RJ quality with higher abundance levels of many bioactive compounds and lower water content. Our data based mainly on RJ metabolic composition constitute evidence for mass production of high-quality RJ.
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http://dx.doi.org/10.1016/j.foodres.2020.109974DOI Listing
January 2021

Distribution, sources, and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in surface sediments from Daya Bay, South China.

Environ Sci Pollut Res Int 2021 Jan 21. Epub 2021 Jan 21.

Marine Bioresources and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, 266061, China.

In this study, the concentrations of 16 kinds of polycyclic aromatic hydrocarbons (PAHs) in surface sediments of Daya Bay were determined and analyzed. Results showed that 16 PAHs were detected in all the samples, and the total PAH concentration ranged from 70.18 to 128.04 ng g, with an average of 103.17 ng g. The cyclic number distribution of PAHs in the sediments was mainly 4 and 5 rings. Six classic PAH ratios named Ant/(Ant + Phe), Fla/(Fla + Pyr), [InP/(InP + BghiP)], [BaA/(BaA + Chr)], BaA/BghiP, and LMW/HMW, and principal component analyses showed that the main source of PAHs in this region was combustion (biomass, coal, and petroleum combustion), and the secondary source was petroleum. The ecological risk analysis of PAHs by using effect range low/median method and mean effects range-median quotient method showed that all of PAHs are lower than the effect range low (ERL) level and the effects range-median quotient (M-ERM-Q) value of all stations is 0.0027-0.0067, with an average value of 0.0046. Thus, it can be seen that PAHs are at a low-risk level in surface sediments of Daya Bay.
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http://dx.doi.org/10.1007/s11356-020-11956-wDOI Listing
January 2021

Vitamin C sensitizes BRAF thyroid cancer to PLX4032 via inhibiting the feedback activation of MAPK/ERK signal by PLX4032.

J Exp Clin Cancer Res 2021 Jan 19;40(1):34. Epub 2021 Jan 19.

Department of Thyroid and Breast Surgery, Peking University Shenzhen Hospital, ShenZhen Peking University-The Hong Kong University of Science and Technology Medical Centre, No.1120, LianHua Road, FuTian district, Shenzhen, 518036, China.

Background: BRAFV600E mutation is the most common mutation in thyroid cancer. It strongly activates MAPK/ERK pathway and indicates an invasive subtype of thyroid cancer. PLX4032 is a selective oral inhibitor of the BRAFV600 kinase although with limited effect in treating this panel of thyroid cancer, due to the feedback activation of MAPK/ERK as well as PI3K/AKT pathways. It was investigated that Vitamin C plays a positive role in inhibiting these pathways in thyroid cancer. However, whether Vitamin C could enhance the antitumor effect of PLX4032 remains largely unclear.

Methods: The antitumor efficacy of combination therapy with PLX4032 and Vitamin C on BRAF thyroid cancer cell was assessed by the MTT assay, EdU assay and colony formation, Chou-Talalay way was employed to analyze the synergistic effect. Flow cytometry were employed to assess cells' apoptosis and cell cycle arrest in response to combination therapy. Xenograft models were used to test its in vivo antitumor activity. Western blot and IHC were applied to investigate the mechanism underlying synergistic effect.

Results: PLX4032 or Vitamin C monotherapy was mildly effective in treating BRAF thyroid cancer cell and xenografts model. The combination therapy significantly inhibited cancer cell proliferation and tumor growth in nude mice, and induced cell apoptosis and cell cycle arrest compared to either monotherapy. PLX4032 monotherapy induced feedback activation of MAPK/ERK as well as PI3K/AKT pathway; while combination therapy significantly relieved this feedback.

Conclusion: Vitamin C promotes the antitumor effect of PLX4032 in BRAF thyroid cancer cell and xenografts model via relieving the feedback activation of MAPK/ERK as well as PI3K/AKT pathway. PLX4032/Vitamin C combination may be a potential therapeutic approach to treat BRAF thyroid cancer.
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http://dx.doi.org/10.1186/s13046-021-01831-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816401PMC
January 2021

The complete chloroplast genome sequence of 'Shuanghong'.

Mitochondrial DNA B Resour 2020 Jun 22;5(3):2537-2538. Epub 2020 Jun 22.

Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

'Shuanghong' is a hybrid offspring of wild grapes. This study first releases the complete chloroplast genome of 'Shuanghong' and subjected the sample to phlogenetic analysis. The chloroplast genome is 161,558 bp in length, and comprises a small single-copy region (19,336 bp) and a large single-copy region (89,744 bp), which are seperated by a pair of inverted repeat regions. The chloroplast genome encodes 133 genes, including 88 CDSs, 8 rRNA genes, and 37 tRNA genes. The phylogenetic tree showed that 'Shuanghong' is most closely related to .
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http://dx.doi.org/10.1080/23802359.2020.1780975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782293PMC
June 2020

Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge.

Sci Rep 2021 Jan 14;11(1):1456. Epub 2021 Jan 14.

College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, China.

In this study, sediments were collected from two different sites in the deep-sea hydrothermal region of the South Atlantic Ocean. Two microbial enrichment cultures (H7S and H11S), which were enriched from the sediments collected at two sample sites, could effectively degrade petroleum hydrocarbons. The bacterial diversity was analyzed by high-throughput sequencing method. The petroleum degradation ability were evaluated by gas chromatography-mass spectrometry and gravimetric analysis. We found that the dominant oil-degrading bacteria of enrichment cultures from the deep-sea hydrothermal area belonged to the genera Pseudomonas, Nitratireductor, Acinetobacter, and Brevundimonas. After a 14-day degradation experiment, the enrichment culture H11S, which was obtained near a hydrothermal vent, exhibited a higher degradation efficiency for alkanes (95%) and polycyclic aromatic hydrocarbons (88%) than the enrichment culture H7S. Interestingly, pristane and phytane as biomarkers were degraded up to 90% and 91% respectively by the enrichment culture H11S, and six culturable oil-degrading bacterial strains were isolated. Acinetobacter junii strain H11S-25, Nitratireductor sp. strain H11S-31 and Pseudomonas sp. strain H11S-28 were used at a density ratio of 95:4:1 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.
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http://dx.doi.org/10.1038/s41598-021-80991-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809451PMC
January 2021

[Serological and molecular study of a novel B(A) allele with multiple missense mutations].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jan;38(1):20-22

Qingdao Blood Center, Shandong 266071, China.

Objective: To explore the molecular basis for an individual suspected as AwB subtype through DNA sequencing.

Methods: ABO serology was carried out with the standard tube method. To identify the ABO gene haplotype, the amplicons of exon 7 were cloned and sequenced.

Results: Serological results showed that the forward typing was AwB and the reverse typing was B. Sequencing analysis revealed that the sample has contained an O01 allele in addition with c.297A>G, c.657C>T, c.796C>A, c.803G>C, c.930G>A variants as compared with the A101 allele.

Conclusion: Through sequencing analysis, the sample with an AwB subtype by serological testing was identified as a novel B(A) phenotype, which was unreported previously.
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http://dx.doi.org/10.3760/cma.j.cn511374-20191126-00605DOI Listing
January 2021

Mechanistic Insight into Royal Protein Inhibiting the Gram-Positive Bacteria.

Biomolecules 2021 01 6;11(1). Epub 2021 Jan 6.

Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

Royal jelly (RJ), a natural honeybee product, has a wide range of antibacterial activities. N-glycosylated major royal jelly protein 2 (N-MRJP2), purified from RJ, can inhibit the growth of (, Gram-positive), a contagious etiological agent of the American foulbrood disease of honeybees. However, the inhibitory mechanism is largely unknown. Antibacterial assay and membrane proteome were conducted to investigate the inhibition capacity of RJ from different instar larvae and treated by N-MRJP2, respectively. The similar antibacterial efficiency of RJ from different larval instar indicates that RJ is vital for the adaptive immune defense of small larvae. The killing of by N-MRJP2 is achieved by disturbing the cell wall biosynthesis, increasing the permeability of cell membrane, hindering aerobic respiration, restraining cell division and inducing cell death. This demonstrates that RJ is critical for the passive immunity of immature larvae and N-MRJP2 can be used as natural antibiotic substance to resist , even for other gram-positive bacteria. This constitutes solid evidence that RJ and N-MRJP2 have potentials as novel antibacterial agents.
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http://dx.doi.org/10.3390/biom11010064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825125PMC
January 2021

Designing future crops: challenges and strategies for sustainable agriculture.

Plant J 2021 Mar 9;105(5):1165-1178. Epub 2021 Jan 9.

National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.

Crop production is facing unprecedented challenges. Despite the fact that the food supply has significantly increased over the past half-century, ~8.9 and 14.3% people are still suffering from hunger and malnutrition, respectively. Agricultural environments are continuously threatened by a booming world population, a shortage of arable land, and rapid changes in climate. To ensure food and ecosystem security, there is a need to design future crops for sustainable agriculture development by maximizing net production and minimalizing undesirable effects on the environment. The future crops design projects, recently launched by the National Natural Science Foundation of China and Chinese Academy of Sciences (CAS), aim to develop a roadmap for rapid design of customized future crops using cutting-edge technologies in the Breeding 4.0 era. In this perspective, we first introduce the background and missions of these projects. We then outline strategies to design future crops, such as improvement of current well-cultivated crops, de novo domestication of wild species and redomestication of current cultivated crops. We further discuss how these ambitious goals can be achieved by the recent development of new integrative omics tools, advanced genome-editing tools and synthetic biology approaches. Finally, we summarize related opportunities and challenges in these projects.
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http://dx.doi.org/10.1111/tpj.15107DOI Listing
March 2021

Rice Protein Tagging Project: A Call for International Collaborations on Genome-wide In-Locus Tagging of Rice Proteins.

Mol Plant 2020 12 12;13(12):1663-1665. Epub 2020 Nov 12.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, USA.

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http://dx.doi.org/10.1016/j.molp.2020.11.006DOI Listing
December 2020