Publications by authors named "Bharath G"

36 Publications

Influence of VKORC1 and CYP2C9 Polymorphisms on Daily Acenocoumarol Dose Requirement in South Indian Patients With Mechanical Heart Valves.

Clin Appl Thromb Hemost 2017 Oct 22;23(7):876-882. Epub 2016 Jun 22.

3 Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, Bangalore, Karnataka, India.

Background And Aim: Chronic rheumatic heart disease (RHD) patients who undergo valve replacement with mechanical valves require lifelong anticoagulation. Acenocoumarol, a vitamin K antagonist has a narrow therapeutic range and wide inter-individual variability. Our aim was to investigate the influence of polymorphisms of VKORC1 and CYP2C9 genes on the mean daily dose requirement of acenocoumarol.

Methods: 205 chronic RHD patients, with mechanical heart valves and on acenocoumarol therapy, were recruited. Genotyping for VKORC1 (-1639G>A and 1173C>T) and CYP2C9 (*2 & *3 alleles) polymorphisms was done by PCR-RFLP. There was complete linkage disequilibrium between VKORC1 polymorphisms (r = 0.98, D' = 1.0, LOD = 74.02). VKORC1 genotype distribution for GG/CC, GA/CT, and AA/TT were 57.6%, 36.1%, and 6.3%, respectively. CYP2C9 genotype distribution for *1/*1, *1/*3, *1/*2, *2/*2, and *2/*3 were 78.5%, 14.1%, 6.3%, 0.5%, and 0.5%, respectively. Patients with a wild type of both VKORC1 (-1639GG and 1173CC) and CYP2C9 gene variants required higher acenocoumarol dose compared to those with mutant genotype ( P = 0.023 and P = 0.008 respectively). On combined genotype analysis, patients having a combination of wild type of VKORC1 with wild type of CYP2C9 (44.4%) required higher daily dose compared to patients bearing heterozygous VKORC1 (-1639GA & 1173CT) with wild type of CYP2C9 (30.2%, P = 0.008).

Conclusion: Presence of a mutant allele of VKORC1 (-1639A & 1173T) and CYP2C9 genes increased the odds of requiring a lower mean dosage of acenocoumarol. Studying the combination of genotypes in RHD patients could predict acenocoumarol dose requirement more accurately.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1076029616655617DOI Listing
October 2017

Synthesis of novel triazole/isoxazole functionalized 7-(trifluoromethyl)pyrido[2,3-d]pyrimidine derivatives as promising anticancer and antibacterial agents.

Bioorg Med Chem Lett 2016 06 16;26(12):2927-2930. Epub 2016 Apr 16.

Bioengineering & Environmental Science, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India.

A series of novel 1,2,3-triazole/isoxazole functionalized pyrido[2,3-d]pyrimidine derivatives 6a-c, 7a-h and 8a-e were prepared in series of synthetic steps. All the compounds screened for the anticancer activity against four human cancer cell lines using Nocodazole as standard. Compounds 7d and 7h showed highest activity against PANC-1 (pancreatic cancer) and A549 (lung cancer) cell lines respectively and more than standard. All the compounds also screened for antibacterial activity using Rifampicin and Ciprofloxacin as standards and identified promising compounds further evaluated for minimum inhibitory concentration to validate the data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmcl.2016.04.038DOI Listing
June 2016

Enzymatic electrochemical glucose biosensors by mesoporous 1D hydroxyapatite-on-2D reduced graphene oxide.

J Mater Chem B 2015 Feb 9;3(7):1360-1370. Epub 2015 Jan 9.

Department of Nanoscience and Technology, Bharathiar University, Coimbatore 641 046, India.

A novel hydrothermal process was used for the preparation of hydroxyapatite (HAp) nanorods on two-dimensional reduced graphene oxides (RGO). The hydrothermal reaction temperature improves the crystallinity of HAp and partially reduces graphene oxide (GO) to RGO. The crystalline structure, chemical composition and morphology of the prepared nanocomposites were characterized by using various analytical techniques. Nanorods of HAp with a diameter and length of ∼32 and 60-85 nm were grown on basal planes and edges of the layered RGO sheets. The estimated specific surface area and pore-size distribution are 120 m g and 5.6 nm, respectively. We also report the direct electrochemistry of glucose oxidase (GOx) on 1D HAp-on-2D RGO nanocomposite-modified glassy carbon electrode (GCE) for glucose sensing. The electrocatalytic and electroanalytical applications of the proposed RGO/HAp/GOx-modified GCE were studied by cyclic voltammetry (CV) and amperometry. The increased electron rate constant of 3.50 s was obtained for the modified GCE. The reported biosensor exhibits a superior detection limit and higher sensitivity ca. 0.03 mM and 16.9 μA mM cm, respectively, with a wide linear range of 0.1-11.5 mM. The tremendous analytical parameters of the reported sensor surpass those of related modified electrodes and are promising for practical industrial applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c4tb01651cDOI Listing
February 2015

Dedifferentiated chondrosarcoma: an aggressive variant of chondrosarcoma.

Asian Cardiovasc Thorac Ann 2015 Feb 4;23(2):221-3. Epub 2014 Feb 4.

Department of Pathology, St. John's Medical College & Hospital, Bangalore, India.

Dedifferentiated chondrosarcomas are a rare and aggressive subtype of chondrosarcoma with a bimorphic pattern on histopathology. Rib is a rare site of dedifferentiated chondrosarcoma. Diagnosis of this subtype preoperatively can be challenging. Treatment options for dedifferentiated chondrosarcoma are limited because they are chemoresistant, and therefore adequate surgery forms the main stay of treatment. We present our experience with a dedifferentiated chondrosarcoma of the rib, and discuss the management of this rare entity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0218492314522253DOI Listing
February 2015

Growth kinetics and immune response of chimeric foot-and-mouth disease virus serotype 'O' produced through replication competent mini genome of serotype Asia 1, 63/72, in BHK cell lines.

Virus Res 2013 May 4;173(2):299-305. Epub 2013 Feb 4.

Indian Veterinary Research Institute, Hebbal, Bangalore 560024, India.

Regular vaccinations with potent vaccine, in endemic countries and vaccination to live in non-endemic countries are the methods available to control foot-and-mouth disease. Selection of candidate vaccine strain is not only cumbersome but the candidate should grow well for high potency vaccine preparation. Alternative strategy is to generate an infectious cDNA of a cell culture-adapted virus and use the replicon for development of tailor-made vaccines. We produced a chimeric 'O' virus in the backbone of Asia 1 and studied its characteristics. The chimeric virus showed high infectivity titre (>10(10)) in BHK 21 cell lines, revealed small plaque morphology and there was no cross reactivity with antiserum against Asia 1. The virus multiplies rapidly and reaches peak at 12h post infection. The vaccine prepared with this virus elicited high antibody titres.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.virusres.2013.01.018DOI Listing
May 2013

Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3.

J Biol Chem 2009 Oct 26;284(43):29956-67. Epub 2009 Aug 26.

Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P. O., Bangalore 560064, India.

The hallmark of mammalian spermiogenesis is the dramatic chromatin remodeling process wherein the nucleosomal histones are replaced by the transition proteins TP1, TP2, and TP4. Subsequently these transition proteins are replaced by the protamines P1 and P2. Hyperacetylation of histone H4 is linked to their replacement by transition proteins. Here we report that TP2 is acetylated in vivo as detected by anti-acetylated lysine antibody and mass spectrometric analysis. Further, recombinant TP2 is acetylated in vitro by acetyltransferase KAT3B (p300) more efficiently than by KAT2B (PCAF). In vivo p300 was demonstrated to acetylate TP2. p300 acetylates TP2 in its C-terminal domain, which is highly basic in nature and possesses chromatin-condensing properties. Mass spectrometric analysis showed that p300 acetylates four lysine residues in the C-terminal domain of TP2. Acetylation of TP2 by p300 leads to significant reduction in its DNA condensation property as studied by circular dichroism and atomic force microscopy analysis. TP2 also interacts with a putative histone chaperone, NPM3, wherein expression is elevated in haploid spermatids. Interestingly, acetylation of TP2 impedes its interaction with NPM3. Thus, acetylation of TP2 adds a new dimension to its role in the dynamic reorganization of chromatin during mammalian spermiogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1074/jbc.M109.052043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785624PMC
October 2009
-->