Publications by authors named "Bereket Molla Tanga"

8 Publications

  • Page 1 of 1

Modified Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes.

Animals (Basel) 2021 Aug 24;11(9). Epub 2021 Aug 24.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus-oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.
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http://dx.doi.org/10.3390/ani11092483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469918PMC
August 2021

The Role of Stem Cells and Their Derived Extracellular Vesicles in Restoring Female and Male Fertility.

Cells 2021 Sep 17;10(9). Epub 2021 Sep 17.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

Infertility is a globally recognized issue caused by different reproductive disorders. To date, various therapeutic approaches to restore fertility have been attempted including etiology-specific medication, hormonal therapies, surgical excisions, and assisted reproductive technologies. Although these approaches produce results, however, fertility restoration is not achieved in all cases. Advances in using stem cell (SC) therapy hold a great promise for treating infertile patients due to their abilities to self-renew, differentiate, and produce different paracrine factors to regenerate the damaged or injured cells and replenish the affected germ cells. Furthermore, SCs secrete extracellular vesicles (EVs) containing biologically active molecules including nucleic acids, lipids, and proteins. EVs are involved in various physiological and pathological processes and show promising non-cellular therapeutic uses to combat infertility. Several studies have indicated that SCs and/or their derived EVs transplantation plays a crucial role in the regeneration of different segments of the reproductive system, oocyte production, and initiation of sperm production. However, available evidence triggers the need to testify the efficacy of SC transplantation or EVs injection in resolving the infertility issues of the human population. In this review, we highlight the recent literature covering the issues of infertility in females and males, with a special focus on the possible treatments by stem cells or their derived EVs.
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http://dx.doi.org/10.3390/cells10092460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468931PMC
September 2021

Cellular Therapy via Spermatogonial Stem Cells for Treating Impaired Spermatogenesis, Non-Obstructive Azoospermia.

Cells 2021 07 14;10(7). Epub 2021 Jul 14.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

Male infertility is a major health problem affecting about 8-12% of couples worldwide. Spermatogenesis starts in the early fetus and completes after puberty, passing through different stages. Male infertility can result from primary or congenital, acquired, or idiopathic causes. The absence of sperm in semen, or azoospermia, results from non-obstructive causes (pretesticular and testicular), and post-testicular obstructive causes. Several medications such as antihypertensive drugs, antidepressants, chemotherapy, and radiotherapy could lead to impaired spermatogenesis and lead to a non-obstructive azoospermia. Spermatogonial stem cells (SSCs) are the basis for spermatogenesis and fertility in men. SSCs are characterized by their capacity to maintain the self-renewal process and differentiation into spermatozoa throughout the male reproductive life and transmit genetic information to the next generation. SSCs originate from gonocytes in the postnatal testis, which originate from long-lived primordial germ cells during embryonic development. The treatment of infertility in males has a poor prognosis. However, SSCs are viewed as a promising alternative for the regeneration of the impaired or damaged spermatogenesis. SSC transplantation is a promising technique for male infertility treatment and restoration of spermatogenesis in the case of degenerative diseases such as cancer, radiotherapy, and chemotherapy. The process involves isolation of SSCs and cryopreservation from a testicular biopsy before starting cancer treatment, followed by intra-testicular stem cell transplantation. In general, treatment for male infertility, even with SSC transplantation, still has several obstacles. The efficiency of cryopreservation, exclusion of malignant cells contamination in cancer patients, and socio-cultural attitudes remain major challenges to the wider application of SSCs as alternatives. Furthermore, there are limitations in experience and knowledge regarding cryopreservation of SSCs. However, the level of infrastructure or availability of regulatory approval to process and preserve testicular tissue makes them tangible and accurate therapy options for male infertility caused by non-obstructive azoospermia, though in their infancy, at least to date.
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http://dx.doi.org/10.3390/cells10071779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304133PMC
July 2021

Improved efficiencies in the generation of multigene-modified pigs by recloning and using sows as the recipient.

Zygote 2021 Jun 28:1-8. Epub 2021 Jun 28.

Department of Microbiology and Immunology; Xenotransplantation Research Center, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea.

This study was performed to improve production efficiency at the level of recipient pig and donor nuclei of transgenic cloned pigs used for xenotransplantation. To generate transgenic pigs, human endothelial protein C receptor (hEPCR) and human thrombomodulin (hTM) genes were introduced using the F2A expression vector into GalT-/-/hCD55+ porcine neonatal ear fibroblasts used as donor cells and cloned embryos were transferred to the sows and gilts. Cloned fetal kidney cells were also used as donor cells for recloning to increase production efficiency. Pregnancy and parturition rates after embryo transfer and preimplantation developmental competence were compared between cloned embryos derived from adult and fetal cells. Significantly higher parturition rates were shown in the group of sows (50.0 vs. 4.1%), natural oestrus (20.8 vs. 0%), and ovulated ovary (16.7 vs. 5.6%) compared with gilt, induced and non-ovulated, respectively (P < 0.05). When using gilts as recipients, final parturitions occurred in only the fetal cell groups and significantly higher blastocyst rates (15.1% vs. 21.3%) were seen (P < 0.05). Additionally, gene expression levels related to pluripotency were significantly higher in the fetal cell group (P < 0.05). In conclusion, sows can be recommended as recipients due to their higher efficiency in the generation of transgenic cloned pigs and cloned fetal cells also can be recommended as donor cells through correct nuclear reprogramming.
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http://dx.doi.org/10.1017/S0967199421000423DOI Listing
June 2021

Enhancing Oocyte Competence With Milrinone as a Phosphodiesterase 3A Inhibitor to Improve the Development of Porcine Cloned Embryos.

Front Cell Dev Biol 2021 30;9:647616. Epub 2021 Apr 30.

Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon, South Korea.

The objective of this study was to investigate the effect of milrinone supplementation as a phosphodiesterase 3A inhibitor during maturation (IVM) to coordinate the cytoplasmic and nuclear maturation of porcine oocytes and subsequent development of porcine cloned embryos. Brilliant cresyl blue (BCB)-stained (BCB +) oocytes, classified as well-developed, and BCB- oocytes were used in parthenogenesis (PA) and cloning, and their preimplantation development was compared. In PA embryos, BCB + oocytes had significantly higher rates of development than BCB- oocytes in terms of maturation (87.5 vs. 71.3%), cleavage (88.6 vs. 76.3%), and blastocyst development (34.3 vs. 25.3%) and also had higher cell numbers (46.9 vs. 38.9%), respectively ( < 0.05). In cloned embryos, the BCB + group also had a significantly higher blastocyst formation rate than the BCB- group (30.6 vs. 20.1%; < 0.05). Supplementation with 75 μM milrinone during IVM of BCB- oocytes showed improvement in maturation and blastocyst development rates, which may be due to the coordinated maturation of the cytoplasm with the nucleus as an effect of milrinone. Moreover, the analysis of nuclear reprogramming via the examination of the expression levels of the reprogramming-related genes , , and in milrinone-supplemented BCB- oocytes showed higher expression levels than that in non-treated BCB- oocytes. These findings demonstrate that milrinone is useful in improving developmental competence in less competent oocytes during IVM and for proper nuclear reprogramming in the production of porcine cloned embryos by coordinating cytoplasmic and nucleus maturation.
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http://dx.doi.org/10.3389/fcell.2021.647616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120234PMC
April 2021

Semen evaluation: methodological advancements in sperm quality-specific fertility assessment - A review.

Anim Biosci 2021 Aug 23;34(8):1253-1270. Epub 2021 Apr 23.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

Assessment of male fertility is based on the evaluation of sperm. Semen evaluation measures various sperm quality parameters as fertility indicators. However, semen evaluation has limitations, and it requires the advancement and application of strict quality control methods to interpret the results. This article reviews the recent advances in evaluating various sperm-specific quality characteristics and methodologies, with the help of different assays to assess sperm-fertility status. Sperm evaluation methods that include conventional microscopic methods, computer-assisted sperm analyzers (CASA), and flow cytometric analysis, provide precise information related to sperm morphology and function. Moreover, profiling fertility-related biomarkers in sperm or seminal plasma can be helpful in predicting fertility. Identification of different sperm proteins and diagnosis of DNA damage has positively contributed to the existing pool of knowledge about sperm physiology and molecular anomalies associated with different infertility issues in males. Advances in methods and sperm-specific evaluation has subsequently resulted in a better understanding of sperm biology that has improved the diagnosis and clinical management of male factor infertility. Accurate sperm evaluation is of paramount importance in the application of artificial insemination and assisted reproductive technology. However, no single test can precisely determine fertility; the selection of an appropriate test or a set of tests and parameters is required to accurately determine the fertility of specific animal species. Therefore, a need to further calibrate the CASA and advance the gene expression tests is recommended for faster and field-level applications.
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http://dx.doi.org/10.5713/ab.21.0072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255896PMC
August 2021

Resveratrol supplementation into extender protects against cryodamage in dog post-thaw sperm.

J Vet Med Sci 2021 Jul 26;83(6):973-980. Epub 2021 Apr 26.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.

Antioxidants have multiple protective roles in a variety of cells and thus can be used to protect sperm against cryo-damage during freezing, which affects fertility. The antioxidant resveratrol (3,5,4-trihydroxytrans-stilbene; RSV) has been reported to protect the animal sperm during cryopreservation, including human sperm. In this study, we assessed the protective effects of RSV supplementation on dog sperm cryopreservation. Semen was collected from four dogs and the effect of different concentrations of RSV (0, 100, 200, and 400 µM) on post-thaw sperm quality was examined. After thawing, sperm motility was assessed using computer-aided sperm analysis, and the structural integrity of the plasma membrane, acrosome, and chromatin was examined. In addition, their mitochondrial activity and gene expression were also assessed. Dog sperm cryopreserved with 200 µM RSV showed significant improvement in post-thaw sperm motility and viability compared with that of the control group (P<0.05). Moreover, RSV-supplemented samples showed significantly higher numbers of sperm with an intact plasma membrane, active mitochondria, and structural integrity of acrosomes and chromatin than that of control samples (P<0.05). Furthermore, gene expression showed that RSV supplemented samples showed lower expression of pro-apoptotic (BAX), reactive oxygen species (ROS) modulator oxidative stress-related (ROMO1) and 8-oxoguanine DNA glycosylase 1 (OGG1) whereas higher expression levels of anti-apoptotic (BCL2), protamine-2 (PRM2), protamine-3 (PRM3) and sperm acrosome-associated 3 (SPACA3) genes than control. Our results suggest that RSV, at its optimum concentration, can be efficiently used as an antioxidant in the cryopreservation of dog sperm.
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http://dx.doi.org/10.1292/jvms.21-0125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267189PMC
July 2021

The First Investigation of Tick Vectors and Tick-Borne Diseases in Extensively Managed Cattle in Alle District, Southwestern Ethiopia.

Vet Med Int 2020 19;2020:8862289. Epub 2020 Dec 19.

Faculty of Veterianry Medicine, Hawassa University, P.O. Box: 05, Hawassa, Ethiopia.

A cross-sectional study was conducted from March 2019 to February 2020 with the objective of identifying ixodid ticks and haemoparasites, in extensively managed livestock, in Alle district, Southwestern Ethiopia. The study area is assumed to be free from ticks, and there had been no diagnostic and treatment options for tick-borne diseases. Among 384 heads of cattle examined for tick infestation and haemoparasites, 139 (36.19%) were infested with one or more tick species and 25 (6.51%) were haemoparasitised. Two genera of ticks, and formerly (), and four species ( and ) were identified. The haemoparasite identified was . Among the risk factors, body condition score and season of the year were found to be significantly associated with tick infestation with  = 9.919, > 0.05 and  = 6.216, > 0.05, respectively, at 95% CI. Tick infestation was found to be significantly associated with haemoparasitemia with  = 22.2 and > 0.05, at 95% CI. The finding of the current study is an alarm ring, as the veterinary service had been not considering any haemoparasitemia in the potential list of differential diagnosis and no treatment inputs have been availed for that purpose. Thus, it is recommended that the veterinary service delivery system in the area should take haemoparasites diagnosis and avail treatment alternatives, particularly tick-borne diseases. Furthermore, there should be a strategical approach in controlling tick-borne diseases in the area before the tick-borne diseases get prevalent and where the control after high prevalence could not be easy in extensive livestock management.
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http://dx.doi.org/10.1155/2020/8862289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769661PMC
December 2020
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