Publications by authors named "Beibei Wang"

270 Publications

Comparative analysis of the miRNA-mRNA regulation networks in turbot (Scophthalmus maximus L.) following Vibrio anguillarum infection.

Dev Comp Immunol 2021 Jun 12:104164. Epub 2021 Jun 12.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

MicroRNAs could not only regulate posttranscriptional silencing of target genes in eukaryotic organisms, but also have positive effect on their target genes as well. These microRNAs have been reported to be involved in intestine mucosal immune responses to pathogen infection in teleost. Therefore, we constructed the immune-related miRNA-mRNA networks in turbot intestine following V. anguillarum infection. In our results, 1,550 differentially expressed (DE) genes and 167 DE miRNAs were identified. 113 DE miRNAs targeting 89 DE mRNAs related to immune response were used to construct miRNA-mRNA interaction networks. Functional analysis showed that target genes were associated with synthesis and degradation of ketone bodies, mucin type O-Glycan biosynthesis, homologous recombination, biotin metabolism, and intestinal immune network for IgA production that were equivalent to the function of IgT and IgM in fish intestine. Finally, 10 differentially expressed miRNAs and 7 differentially expressed mRNAs were selected for validating the accuracy of high-throughput sequencing results by qRT-PCR. The results of this study will provide valuable information for the elucidation of the regulation mechanisms of miRNA-mRNA interactions involved in disease resistance in teleost intestine mucosal immune system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dci.2021.104164DOI Listing
June 2021

Identification and characterization of a C-type lectin in turbot (Scophthalmus maximus) which functioning as a pattern recognition receptor that binds and agglutinates various bacteria.

Fish Shellfish Immunol 2021 May 29;115:104-111. Epub 2021 May 29.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China. Electronic address:

C-type lectins (CTLs) are important pathogen pattern recognition receptors that recognize carbohydrate structures. In present study, a C-type lectin domain family 4 member E-like gene from turbot, which tentatively named SmCLEC4E-like (SmCLEC4EL), was identified, and the expressional and functional analyses were performed. In our results, SmCLEC4EL showed conserved synteny with CLEC4E-like genes from several fish species in genome, and possessed a typical type II transmembrane CTL architecture: an N-terminal intracellular region, a transmembrane domain and a C-terminal extracellular region which contained a predicted carbohydrate recognition domain (CRD). In addition, SmCLEC4EL exhibited the highest expression level in spleen in healthy fish, and showed significantly induced expression in mucosal tissues, intestine and skin, under bacteria challenge. Finally, the recombinant SmCLEC4EL protein combined with LPS, PGN, LTA and five different kinds of bacteria in a dose-dependent manner, and agglutinated these bacteria strains in the presence of calcium. These findings collectively demonstrated that SmCLEC4EL, a calcium-dependent CTL, could function as a pattern recognition receptor in pathogen recognition and participate in host anti-bacteria immunity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2021.05.020DOI Listing
May 2021

Characterization of toll-like receptor 1 (TLR1) in turbot (Scophthalmus maximus L.).

Fish Shellfish Immunol 2021 May 27;115:27-34. Epub 2021 May 27.

School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

TLRs are the first and best-characterized pattern recognition receptors conserved across all the species. Different from mammals, the TLRs in teleost fishes are very diversified due to various evolutionary mechanisms. Here, we characterized one TLR1 gene in turbot, with a 2,415 bp open reading frame (ORF), that encoding 804 amino acid residues, and have the highest similarity and identity both to Paralichthys olivaceus with 88.9% and 79.9%. In phylogenetic analysis, it was firstly clustered with P. olivaceus, and then clustered with Takifugu rubripes. TLR1 was widely expressed in all the examined healthy tissues with the highest expression level in spleen, followed by head-kidney. In addition, it was significantly regulated in gill, skin and intestine following Edwardsiella tarda and Vibrio anguillarum challenge with different expression patterns. In in vitro stimulation with pathogen-associated molecular patterns, TLR1 showed significantly strong and elevated responses to LPS, but only responded to LTA and Poly(I:C) at the highest evaluated concentration, while no response was detected using PGN stimulation. Moreover, in subcellular localization analysis, TLR1 was distributed in the cytoplasm, membrane and nucleus. Taken together, TLR1 played vital roles for host immune response to bacterial infection, only with strong binding ability to LPS and involved in the production of inflammatory cytokines. However, the specific ligand for TLR1 and its functional association with other TLRs should be further characterized in fish species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2021.05.018DOI Listing
May 2021

The Application of an Adaptive Genetic Algorithm Based on Collision Detection in Path Planning of Mobile Robots.

Comput Intell Neurosci 2021 7;2021:5536574. Epub 2021 May 7.

Tianjin Keyvia Electric Co., Ltd., Tianjin 300384, China.

An adaptive genetic algorithm based on collision detection (AGACD) is proposed to solve the problems of the basic genetic algorithm in the field of path planning, such as low convergence path quality, many iterations required for convergence, and easily falling into the local optimal solution. First, this paper introduces the Delphi weight method to evaluate the weight of path length, path smoothness, and path safety in the fitness function, and a collision detection method is proposed to detect whether the planned path collides with obstacles. Then, the population initialization process is improved to reduce the program running time. After comprehensively considering the population diversity and the number of algorithm iterations, the traditional crossover operator and mutation operator are improved, and the adaptive crossover operator and adaptive mutation operator are proposed to avoid the local optimal solution. Finally, an optimization operator is proposed to improve the quality of convergent individuals through the second optimization of convergent individuals. The simulation results show that the adaptive genetic algorithm based on collision detection is not only suitable for simulation maps with various sizes and obstacle distributions but also has excellent performance, such as greatly reducing the running time of the algorithm program, and the adaptive genetic algorithm based on collision detection can effectively solve the problems of the basic genetic algorithm.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5536574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124003PMC
May 2021

Antibody evasion by the P.1 strain of SARS-CoV-2.

Cell 2021 05 30;184(11):2939-2954.e9. Epub 2021 Mar 30.

Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Terminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses to the virus spike derived from early isolates. However, new strains have emerged with multiple mutations, including P.1 from Brazil, B.1.351 from South Africa, and B.1.1.7 from the UK (12, 10, and 9 changes in the spike, respectively). All have mutations in the ACE2 binding site, with P.1 and B.1.351 having a virtually identical triplet (E484K, K417N/T, and N501Y), which we show confer similar increased affinity for ACE2. We show that, surprisingly, P.1 is significantly less resistant to naturally acquired or vaccine-induced antibody responses than B.1.351, suggesting that changes outside the receptor-binding domain (RBD) impact neutralization. Monoclonal antibody (mAb) 222 neutralizes all three variants despite interacting with two of the ACE2-binding site mutations. We explain this through structural analysis and use the 222 light chain to largely restore neutralization potency to a major class of public antibodies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cell.2021.03.055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008340PMC
May 2021

Unveiling the photoluminescence regulation of colloidal perovskite quantum dots via defect passivation and lattice distortion by potassium cations doping: Not the more the better.

J Colloid Interface Sci 2021 Aug 24;596:199-205. Epub 2021 Mar 24.

Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin 300354, China. Electronic address:

In this work, we have first demonstrated that the potassium cation doping effect on photoluminescence (PL) regulation of CHNHPbBr (CHNH=MA) colloidal perovskite quantum dots (QDs) is significantly different from the other alkali cation doping effects. The PL intensity will be generally enhanced with the increase doping amounts of other alkali cations. Herein, we have unveiled that the PL of the potassium-doped perovskite QDs is initially prompted by the potassium ions doping and then inhibited with further growing doping amount of the potassium ions. Furthermore, we have also demonstrated that the PL inhibition phenomenon is ascribed as quick trapping of redundant photogenerated electrons by the trap states after huge amount doping besides defect passivation and octahedral structure distortion induced by the initial doping. At the same time, the specific excited state transient absorption and the lifetime of MAKPbBr also confirm that the radiation recombination process is enhanced via defect passivation and lattice distortion, which is induced by moderate potassium cations doping. In addition, the PL of colloidal perovskite quantum dots can be adjusted from orange to cyan within the wavelength range of 300 nm - 600 nm and exhibit better stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.03.128DOI Listing
August 2021

Metabolic Analysis Reveals Gene Transformation Does Not Affect the Sensitivity of Rice to Rice Dwarf Virus.

Metabolites 2021 Mar 30;11(4). Epub 2021 Mar 30.

State Key Laboratory of Rice Biology & Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

Metabolomics is beginning to be used for assessing unintended changes in genetically modified (GM) crops. To investigate whether gene transformation would induce metabolic changes in rice plants, and whether the metabolic changes would pose potential risks when rice plants are exposed to rice dwarf virus (RDV), the metabolic profiles of rice T1C-19 and its non- parental rice MH63 under RDV-free and RDV-infected status were analyzed using gas chromatography-mass spectrometry (GC-MS). Compared to MH63 rice, slice difference was detected in T1C-19 under RDV-free conditions (less than 3%), while much more metabolites showed significant response to RDV infection in T1C-19 (15.6%) and in MH63 (5.0%). Pathway analysis showed biosynthesis of lysine, valine, leucine, and isoleucine may be affected by RDV infection in T1C-19. No significant difference in the contents of free amino acids (AAs) was found between T1C-19 and MH63 rice, and the free AA contents of the two rice plants showed similar responses to RDV infection. Furthermore, no significant differences of the RDV infection rates between T1C-19 and MH63 were detected. Our results showed the gene transformation did not affect the sensitivity of rice to RDV, indicating rice would not aggravate the epidemic and dispersal of RDV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/metabo11040209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065979PMC
March 2021

GeCNs: Graph Elastic Convolutional Networks for Data Representation.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 2;PP. Epub 2021 Apr 2.

Graph representation and learning is a fundamental problem in machine learning area. Graph Convolutional Networks (GCNs) have been recently studied and demonstrated very powerful for graph representation and learning. Graph convolution (GC) operation in GCNs can be regarded as a composition of feature aggregation and nonlinear transformation step. Existing GCs generally conduct feature aggregation on a full neighborhood set in which each node computes its representation by aggregating the feature information of all its neighbors. However, this full aggregation strategy is not guaranteed to be optimal for GCN learning and also can be affected by some graph structure noises, such as incorrect or undesired edge connections. To address these issues, we propose to integrate elastic net based selection into graph convolution and propose a novel graph elastic convolution (GeC) operation. In GeC, each node can adaptively select the optimal neighbors in its feature aggregation. The key aspect of the proposed GeC operation is that it can be formulated by a regularization framework, based on which we can derive a simple update rule to implement GeC in a self-supervised manner. Using GeC, we then present a novel GeCN for graph learning. Experimental results demonstrate the effectiveness and robustness of GeCN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3070599DOI Listing
April 2021

FGF1 prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression.

Signal Transduct Target Ther 2021 Mar 24;6(1):133. Epub 2021 Mar 24.

School of Pharmaceutical Sciences and Center for Structural Biology, Wenzhou Medical University, Wenzhou, Zhejiang, China.

As a classically known mitogen, fibroblast growth factor 1 (FGF1) has been found to exert other pleiotropic functions such as metabolic regulation and myocardial protection. Here, we show that serum levels of FGF1 were decreased and positively correlated with fraction shortening in diabetic cardiomyopathy (DCM) patients, indicating that FGF1 is a potential therapeutic target for DCM. We found that treatment with a FGF1 variant (FGF1) with reduced proliferative potency prevented diabetes-induced cardiac injury and remodeling and restored cardiac function. RNA-Seq results obtained from the cardiac tissues of db/db mice showed significant increase in the expression levels of anti-oxidative genes and decrease of Nur77 by FGF1 treatment. Both in vivo and in vitro studies indicate that FGF1 exerted these beneficial effects by markedly reducing mitochondrial fragmentation, reactive oxygen species (ROS) generation and cytochrome c leakage and enhancing mitochondrial respiration rate and β-oxidation in a 5' AMP-activated protein kinase (AMPK)/Nur77-dependent manner, all of which were not observed in the AMPK null mice. The favorable metabolic activity and reduced proliferative properties of FGF1 testify to its promising potential for use in the treatment of DCM and other metabolic disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-021-00542-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991671PMC
March 2021

The antigenic anatomy of SARS-CoV-2 receptor binding domain.

Cell 2021 04 18;184(8):2183-2200.e22. Epub 2021 Feb 18.

MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, UK.

Antibodies are crucial to immune protection against SARS-CoV-2, with some in emergency use as therapeutics. Here, we identify 377 human monoclonal antibodies (mAbs) recognizing the virus spike and focus mainly on 80 that bind the receptor binding domain (RBD). We devise a competition data-driven method to map RBD binding sites. We find that although antibody binding sites are widely dispersed, neutralizing antibody binding is focused, with nearly all highly inhibitory mAbs (IC < 0.1 μg/mL) blocking receptor interaction, except for one that binds a unique epitope in the N-terminal domain. Many of these neutralizing mAbs use public V-genes and are close to germline. We dissect the structural basis of recognition for this large panel of antibodies through X-ray crystallography and cryoelectron microscopy of 19 Fab-antigen structures. We find novel binding modes for some potently inhibitory antibodies and demonstrate that strongly neutralizing mAbs protect, prophylactically or therapeutically, in animal models.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cell.2021.02.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891125PMC
April 2021

Antibody testing for COVID-19: A report from the National COVID Scientific Advisory Panel.

Wellcome Open Res 2020 11;5:139. Epub 2020 Jun 11.

Oxford University Hospitals NHS Foundation Trust, Oxford, OX3 9DU, UK.

The COVID-19 pandemic caused >1 million infections during January-March 2020. There is an urgent need for reliable antibody detection approaches to support diagnosis, vaccine development, safe release of individuals from quarantine, and population lock-down exit strategies. We set out to evaluate the performance of ELISA and lateral flow immunoassay (LFIA) devices. We tested plasma for COVID (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) IgM and IgG antibodies by ELISA and using nine different LFIA devices. We used a panel of plasma samples from individuals who have had confirmed COVID infection based on a PCR result (n=40), and pre-pandemic negative control samples banked in the UK prior to December-2019 (n=142). ELISA detected IgM or IgG in 34/40 individuals with a confirmed history of COVID infection (sensitivity 85%, 95%CI 70-94%), vs. 0/50 pre-pandemic controls (specificity 100% [95%CI 93-100%]). IgG levels were detected in 31/31 COVID-positive individuals tested ≥10 days after symptom onset (sensitivity 100%, 95%CI 89-100%). IgG titres rose during the 3 weeks post symptom onset and began to fall by 8 weeks, but remained above the detection threshold. Point estimates for the sensitivity of LFIA devices ranged from 55-70% versus RT-PCR and 65-85% versus ELISA, with specificity 95-100% and 93-100% respectively. Within the limits of the study size, the performance of most LFIA devices was similar. Currently available commercial LFIA devices do not perform sufficiently well for individual patient applications. However, ELISA can be calibrated to be specific for detecting and quantifying SARS-CoV-2 IgM and IgG and is highly sensitive for IgG from 10 days following first symptoms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12688/wellcomeopenres.15927.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941096PMC
June 2020

Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera.

Cell 2021 04 18;184(8):2201-2211.e7. Epub 2021 Feb 18.

NIHR Oxford Biomedical Research Centre, Oxford, UK; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.

SARS-CoV-2 has caused over 2 million deaths in little over a year. Vaccines are being deployed at scale, aiming to generate responses against the virus spike. The scale of the pandemic and error-prone virus replication is leading to the appearance of mutant viruses and potentially escape from antibody responses. Variant B.1.1.7, now dominant in the UK, with increased transmission, harbors 9 amino acid changes in the spike, including N501Y in the ACE2 interacting surface. We examine the ability of B.1.1.7 to evade antibody responses elicited by natural SARS-CoV-2 infection or vaccination. We map the impact of N501Y by structure/function analysis of a large panel of well-characterized monoclonal antibodies. B.1.1.7 is harder to neutralize than parental virus, compromising neutralization by some members of a major class of public antibodies through light-chain contacts with residue 501. However, widespread escape from monoclonal antibodies or antibody responses generated by natural infection or vaccination was not observed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cell.2021.02.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891044PMC
April 2021

Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera.

Cell 2021 04 23;184(9):2348-2361.e6. Epub 2021 Feb 23.

NIHR Oxford Biomedical Research Centre, Oxford, UK; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.

The race to produce vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began when the first sequence was published, and this forms the basis for vaccines currently deployed globally. Independent lineages of SARS-CoV-2 have recently been reported: UK, B.1.1.7; South Africa, B.1.351; and Brazil, P.1. These variants have multiple changes in the immunodominant spike protein that facilitates viral cell entry via the angiotensin-converting enzyme-2 (ACE2) receptor. Mutations in the receptor recognition site on the spike are of great concern for their potential for immune escape. Here, we describe a structure-function analysis of B.1.351 using a large cohort of convalescent and vaccinee serum samples. The receptor-binding domain mutations provide tighter ACE2 binding and widespread escape from monoclonal antibody neutralization largely driven by E484K, although K417N and N501Y act together against some important antibody classes. In a number of cases, it would appear that convalescent and some vaccine serum offers limited protection against this variant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cell.2021.02.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901269PMC
April 2021

Long-term exposure to ambient PM increase obesity risk in Chinese adults: A cross-sectional study based on a nationwide survey in China.

Sci Total Environ 2021 Jul 2;778:145812. Epub 2021 Mar 2.

Nicholas School of the Environment and Global Health Institute, Duke University, Durham, NC, USA; Duke Kunshan University, Kunshan, Jiangsu Province, China.

Certain studies suggest that air pollution could be a risk factor for obesity, but the evidence on the association between air pollution exposure and obesity in adults is limited. This study aims to examine the association between long-term exposure to fine particulate matter (PM) and obesity-related traits in Chinese adults. Thus, a cross-sectional study was conducted based on a nationally representative sample of 91, 121 adults from 31 provinces in China. Integrated the data from satellites, chemical transport model, and ground observations, annual average concentrations of PM was obtained at the township level using a machine learning method. The information on body weight, height, and waist circumference (WC) were obtained from a questionnaire survey. The general obesity and abdominal obesity status were classified based on body mass index (BMI) and WC, respectively. Logistic and multivariate linear regression models were used to examine the association between PM and obesity-related traits, along with the examination of potential effect modifications. After adjustment for covariates, a 10 μg/m increase in PM concentration was associated with 8.0% [95% confidence interval (CI): 1.0%, 10.0%] and 10% (95% CI: 9.0%, 11.0%) increases in odds for general obesity and abdominal obesity, respectively. The odds ratios associated with per 10 μg/m PM increase were significantly greater in individuals of older age (≥60 years), of Han ethnicity, with lower socioeconomic status (SES), cooking without using a ventilation device, using unclean household fuels, having near-home pollution sources, and doing no physical exercise. These findings suggest that long-term exposure to ambient PM increase obesity risk in Chinese adults. It has significant significance to reduce air pollution to reducing the burden of obesity, particularly for the susceptible populations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.145812DOI Listing
July 2021

pH-controlled mechanism of photocatalytic RhB degradation over g-CN under sunlight irradiation.

Photochem Photobiol Sci 2021 Feb 20;20(2):303-313. Epub 2021 Feb 20.

School of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, 453003, China.

Photocatalysis of dye degradation is one of green and cheap technologies for solving environmental pollution. Whereas it is rarely concerned that the degradation process varied with the change of solution condition, this work studied the influence of hydrion in the solution on the photodegradation process of Rhodamine B (RhB) over g-CN. The photocatalytic activity of RhB degradation was enhanced gradually with increased hydrion content in the system. The efficiency for RhB degradation over g-CN in weak acidic system with interference of multiple metal-ions still reached near 95% after 30 min of natural sunlight irradiation. A large amount of oxidation species and the hydroxylation mineralization process were induced by increasing the hydrion concentration. Two degradation processes for deethylation of four ethyl groups and the direct chromophoric degradation were discovered and proved by multifarious intermediates in different systems using the ESR technique, LC/MS and GC/MS analysis. In addition, the photosensitization played a critical role in the RhB degradation. A feasible degradation mechanism was proposed for the RhB degradation based on the experimental results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43630-021-00019-9DOI Listing
February 2021

Heterogeneous Synergetic Effect of Metal-Oxide Interfaces for Efficient Hydrogen Evolution in Alkaline Solutions.

ACS Appl Mater Interfaces 2021 Mar 15;13(11):13838-13847. Epub 2021 Mar 15.

State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

Water dissociation in alkaline solutions is one of the biggest challenges in hydrogen evolution reactions (HERs). The key is to obtain a catalyst with optimal and balanced OH adsorption energy and H adsorption/H desorption energy. Herein, we synthesized a NiW/WO catalyst on the Ni foam that optimized the coverage and size of NiW alloys decorated on the NiWO/WO substrate. Our experiments showed that NiW-NiWO interfaces could accelerate water dissociation, and NiW-WO interfaces facilitate adsorbed H atoms spillover and H desorption. In addition, we applied a suite of characterization techniques to analyze surface evolution processes in catalysts under various cathodic potentials so as to illustrate the competition between chemical oxidation and electrochemical reduction reactions. The results demonstrated that high coverage of large NiW nanoparticles resulted in a greater stable interface. The two efficient interfaces synergetically promote the Volmer-Tafel reaction. NiW/WO catalysts exhibited extraordinary HER activity with a low overpotential of 48 mV at a 10 mA cm current density and a Tafel slope of 33 mV dec. This work has shown that low-cost catalysts with proper hierarchical interfaces can be engineered and can be optimized into a tandem system, which will significantly promote HER activity in alkaline electrolytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c00945DOI Listing
March 2021

A graphene assembled porous fiber-based Janus membrane for highly effective solar steam generation.

J Colloid Interface Sci 2021 Jun 20;592:77-86. Epub 2021 Feb 20.

State Key Laboratory of Photon-Technology in Western China Energy, International Collaborative Center on Photoelectric Technology and Nano Functional Materials, Institute of Photonics & Photon-Technology, Northwest University, Xi'an 710127, PR China; Shaanxi Joint Lab of Graphene (NWU), Xi'an 710127, PR China. Electronic address:

Owing to the shortage of clean water as the global problem, the exploration of photothermal substances with high performance solar steam generation for sustainable water purification is essential and urgent. Herein, we demonstrate the assembly of two-dimensional graphene into one-dimensional rough, loose, and porous fibers and further use the assembled fibers to fabricate Janus membrane evaporator. The specific configuration guarantees an enhanced light harvesting property through multiple reflections, and improves the vapor transport ability through the constructed interlaced network. As a result, the as-obtained evaporator exhibits high solar absorbance, superior photothermal property and energy conversion efficiency, which is much higher than those of other reported Janus membrane evaporators and also better than the fabricated carbon nanotube-, and graphene sheet-based Janus membrane evaporator. The water purification results indicate that the fabricated graphene fiber-based Janus membrane is highly effective in seawater desalination without obvious salt accumulation and heavy metal wastewater purification. This study proposes a neotype graphene assembly for the fabrication of Janus membrane evaporator, which has potential applications in desalination and wastewater decontamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.02.045DOI Listing
June 2021

Color-tunable and Highly Emissive Solid Materials Constructed from Tetraphenylethylene-o-carborane-based Building Blocks: Synthesis, Aggregation-induced emission, and Photophysics.

Chem Asian J 2021 Apr 9;16(7):757-760. Epub 2021 Mar 9.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.

A novel kind of expanded tetraphenylethylene (TPE)-carborane-TPE pentad has been synthesized by using two adjacent carborane moieties as central bridges and three TPE units in lateral positions. Its solid-state fluorescence quantum yield was substantially increased to 68.2% by expanding the number of bridges between carborane and TPE. Subsequently, the emission color shifted from blue to orange-yellow (126 nm). Mechanical insights into the electronic structure of the extended TPE-carborane-TPE pentads were obtained from density functional theory (DFT) calculations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/asia.202100150DOI Listing
April 2021

Is hand-to-mouth contact the main pathway of children's soil and dust intake?

Environ Geochem Health 2021 Feb 12. Epub 2021 Feb 12.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.

Children (n = 240) between the ages of 2 and 17 years were randomly selected from three cities in China. The total amount of soil and dust (SD) on their hands was measured and ranged from 3.50-187.39 mg (median = 19.49 mg). We screened for seven elements (Ce, V, Y, Al, Ba, Sc, and Mn), and Ce levels were used to calculate hand SD by variability and soil elements. The main factors affecting SD amount were location and age group, as identified using a conditional inference tree. Hand SD and the hand SD intake rate were highest in Gansu Province, followed by Guangdong and Hubei provinces, respectively. Hand SD and the hand SD intake rate were highest among children in primary school, followed by kindergarten and secondary school, respectively. The hand SD intake rate of the three typical areas was 11.9 mg/d, which was about 26.6% of the children's soil intake rate (44.8 mg/d), indicating that hand-to-mouth contact is not the main route for children's soil intake in the three areas of China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10653-021-00830-4DOI Listing
February 2021

Microwave-Assisted Air Epoxidation of Mixed Biolefins over a Spherical Bimetal ZnCo-MOF Catalyst.

ACS Appl Mater Interfaces 2021 Feb 11;13(7):8474-8487. Epub 2021 Feb 11.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials & Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & Ministry-of-Education Key Laboratory for Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, P. R. China.

Here, we report the synthesis of spherical bimetal ZnCo-MOF materials by a hydrothermal rotacrystallization method and their catalytic activity on the air epoxidation of mixed biolefins enhanced by microwaves. The structural and chemical properties of the ZnCo-MOF materials were fully characterized by XRD, IR, SEM, TG, XPS, and NH-TPD. The morphology of the material exhibited a three-dimensional spherical structure. From an NH-TPD test of the ZnCo-MOF catalyst, it could be concluded that the ZnCo-MOF-H-150 rpm material had the highest acidic content and the strongest acidity among the catalysts synthesized by different methods, which gave the best performance in the epoxidation of mixed biolefins. The air epoxidation reaction was carried out under atmospheric pressure and microwave conditions, in the absence of any initiator or coreducing agent. Moreover, the ZnCo-MOF catalyst could be recycled six times without reducing the catalytic activity significantly, which showed the stability of spherical catalyst material under microwaves.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c22317DOI Listing
February 2021

BioMaster: An Integrated Database and Analytic Platform to Provide Comprehensive Information About BioBrick Parts.

Front Microbiol 2021 21;12:593979. Epub 2021 Jan 21.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Synthetic biology seeks to create new biological parts, devices, and systems, and to reconfigure existing natural biological systems for custom-designed purposes. The standardized BioBrick parts are the foundation of synthetic biology. The incomplete and flawed metadata of BioBrick parts, however, are a major obstacle for designing genetic circuit easily, quickly, and accurately. Here, a database termed BioMaster http://www.biomaster-uestc.cn was developed to extensively complement information about BioBrick parts, which includes 47,934 items of BioBrick parts from the international Genetically Engineered Machine (iGEM) Registry with more comprehensive information integrated from 10 databases, providing corresponding information about functions, activities, interactions, and related literature. Moreover, BioMaster is also a user-friendly platform for retrieval and analyses of relevant information on BioBrick parts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.593979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858672PMC
January 2021

Structural basis for self-cleavage prevention by tag:anti-tag pairing complementarity in type VI Cas13 CRISPR systems.

Mol Cell 2021 03 19;81(5):1100-1115.e5. Epub 2021 Jan 19.

State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China. Electronic address:

Bacteria and archaea apply CRISPR-Cas surveillance complexes to defend against foreign invaders. These invading genetic elements are captured and integrated into the CRISPR array as spacer elements, guiding sequence-specific DNA/RNA targeting and cleavage. Recently, in vivo studies have shown that target RNAs with extended complementarity with repeat sequences flanking the target element (tag:anti-tag pairing) can dramatically reduce RNA cleavage by the type VI-A Cas13a system. Here, we report the cryo-EM structure of Leptotrichia shahii LshCas13a in complex with target RNA harboring tag:anti-tag pairing complementarity, with the observed conformational changes providing a molecular explanation for inactivation of the composite HEPN domain cleavage activity. These structural insights, together with in vitro biochemical and in vivo cell-based assays on key mutants, define the molecular principles underlying Cas13a's capacity to target and discriminate between self and non-self RNA targets. Our studies illuminate approaches to regulate Cas13a's cleavage activity, thereby influencing Cas13a-mediated biotechnological applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molcel.2020.12.033DOI Listing
March 2021

Excitons competition regulation via organic cation-site and halogen-site co-halogenation of (X-p-PEA)Pb(Cl/Br) perovskites.

J Colloid Interface Sci 2021 Apr 28;588:494-500. Epub 2020 Dec 28.

Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, National Demonstration Center for Experimental Chemistry & Chemical Engineering Education, School of Science, Tianjin University, Tianjin 300354, China. Electronic address:

In this work, we report a family of co-halogenated two-dimensional hybrid perovskites (2DHPs) based on phenethylammonium lead halogen ((PEA)Pb(Cl/Br)) in which the organic cation-site (PEA) is substituted with halogen at the para-site, namely the formation of 4-halophenethylamine (X-p-PEA) (X = Cl, Br; p: para-site). The organic cations are regulated by introducing halogen ions at the para-site of the benzene ring to promote the structural distortion of the lead halide octahedral inorganic layer. Furthermore, (X-p-PEA) causes a shift in the energy band distribution of 2DHPs. In this case, the photoluminescence competition of free excitons (FEs) and self-trapped excitons (STEs) changes the microscopic relaxation process of excitons. In addition, we found that (Br-p-PEA) can increase the photoluminescence quantum yield (PLQY). At the same time, we regulate the halogen-site of perovskites from lead-chloride perovskites (LCPs) to lead bromine perovskites (LBPs), achieving emission from white light to blue light. Therefore, the co-halogenation regulation strategy of organic cation-site and halogen-site can effectively regulate the photoluminescence wavelength and improve the PLQY. This is of great significance for the development of perovskite materials with specific optoelectronic applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.12.094DOI Listing
April 2021

Melatonin provides protection against cisplatin-induced ovarian damage and loss of fertility in mice.

Reprod Biomed Online 2021 Mar 8;42(3):505-519. Epub 2020 Oct 8.

Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan Hubei, China. Electronic address:

Research Question: Can melatonin provide non-invasive ovarian protection against damage caused by cis-diamminedichloroplatinum (cisplatin) and preserve fertility in female cancer patients? And if so, what is the possible mechanism?

Design: Athymic BALB/c nude tumour-bearing female mice were used to demonstrate whether melatonin affects the antineoplastic effect when co-administrated with cisplatin. Sexually mature and newborn C57BL/6 female mice were used to evaluate the potential effects of melatonin on the ovarian follicle pool, pregnancy rate and litter number in cisplatin-treated mice. The ovaries underwent immunohistochemical, TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) and gene array analysis to explore the underlying mechanism. In addition, granulosa cells were isolated to investigate the potential protective mechanism of melatonin.

Results: Melatonin not only enhanced the anti-cancer effect of cisplatin in tumour-bearing nude mice, but also reduced ovarian toxicity and preserved long-term fertility in cisplatin-treated C57BL/6 female mice. When co-administrated, melatonin was able to reduce the DNA damage and toxic effects on lipid peroxidation in the ovaries caused by cisplatin. Specifically, melatonin was able to largely restore lipid peroxidation in granulosa cells and thus prevent ovarian follicles from being depleted.

Conclusions: Melatonin has the potential to be used as a chemotherapeutic adjuvant to simultaneously improve the outcome of anti-cancer treatment and preserve ovarian function during cisplatin chemotherapy. Notably, its properties of DNA protection and antioxidant effects on follicles may benefit female cancer survivors and prevent premature ovarian failure as well as fertility loss caused by chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rbmo.2020.10.001DOI Listing
March 2021

Endocytic Adaptors in Cardiovascular Disease.

Front Cell Dev Biol 2020 11;8:624159. Epub 2020 Dec 11.

Vascular Biology Program, Boston Children's Hospital, Boston, MA, United States.

Endocytosis is the process of actively transporting materials into a cell by membrane engulfment. Traditionally, endocytosis was divided into three forms: phagocytosis (cell eating), pinocytosis (cell drinking), and the more selective receptor-mediated endocytosis (clathrin-mediated endocytosis); however, other important endocytic pathways (e.g., caveolin-dependent endocytosis) contribute to the uptake of extracellular substances. In each, the plasma membrane changes shape to allow the ingestion and internalization of materials, resulting in the formation of an intracellular vesicle. While receptor-mediated endocytosis remains the best understood pathway, mammalian cells utilize each form of endocytosis to respond to their environment. Receptor-mediated endocytosis permits the internalization of cell surface receptors and their ligands through a complex membrane invagination process that is facilitated by clathrin and adaptor proteins. Internalized vesicles containing these receptor-ligand cargoes fuse with early endosomes, which can then be recycled back to the plasma membrane, delivered to other cellular compartments, or destined for degradation by fusing with lysosomes. These intracellular fates are largely determined by the interaction of specific cargoes with adaptor proteins, such as the epsins, disabled-homolog 2 (Dab2), the stonin proteins, epidermal growth factor receptor substrate 15, and adaptor protein 2 (AP-2). In this review, we focus on the role of epsins and Dab2 in controlling these sorting processes in the context of cardiovascular disease. In particular, we will focus on the function of epsins and Dab2 in inflammation, cholesterol metabolism, and their fundamental contribution to atherogenicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2020.624159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759532PMC
December 2020

Detection of functional microorganisms in benzene [a] pyrene-contaminated soils using DNA-SIP technology.

J Hazard Mater 2021 04 7;407:124788. Epub 2020 Dec 7.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

DNA-SIP technology was used to detect active BaP-degraders involved in the biodegradation of benzo [a] pyrene (BaP) in two soils separately and in mixture. The lowest BaP removal was observed in red soil, and Ramlibacter (OTU830) belonging to the γ-Proteobacteria was labeled as BaP degrader with C-BaP. The highest diversity of degrading microorganisms occurred in the paddy soil with OTUs belonging to Nocardioids, Micromonospora, Saccharothrix, Lysobacter and Methylium present and a BaP removal efficiency of 29.5% after 14 d. BaP degraders in the mixed microbiome of the soil mixture were Burkholderia and Phenylobacterium, together with Nocardioides and Micromonospora as in the paddy soil. These results indicated that the active BaP-degraders in the mixed microbiome were identical to the active BaP-degraders in paddy soil (OTU356 and OTU328), but also unique in the mixed microbiome, such as OTU393 and OTU392. The functional genes of PAH-ring hydroxylating dioxygenases (PAH-RHD) were expressed and were positively related to the removal of BaP, and the active BaP degrading bacteria included both Gram-positive and Gram-negative bacteria. Saccharothrix, Phylobacterium, Micromonospora and Nocardioids are here reported as BaP degraders for the first time using DNA-SIP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124788DOI Listing
April 2021

Construction of a mitochondria-targeted ratiometric fluorescent probe for monitoring hydrazine in soil samples and culture cells.

J Hazard Mater 2021 03 28;406:124589. Epub 2020 Nov 28.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, Jiangnan University, Wuxi, Jiangsu, China. Electronic address:

Isoniazid and its major metabolite, hydrazine (NH), may interfere with mitochondrial function and have negative effects on cells. Consequently, an understanding of the role of NH in mitochondria is highly desirable for protecting human health. Herein, we report a novel mitochondria-targeted ratiometric fluorescent probe (Mitro-NH) for NH detection. Mitro-NH exhibited an attenuation of green emission at 521 nm and an enhancement of yellow emission at 590 nm in the presence of NH because of hydrazinolysis, indicating that it can be used as a ratiometric chemosensor for NH with high selectivity and sensitivity. Such on-site monitoring of NH vapour using test strips and NH-moistened soil analysis demonstrated its advantages in potential application for the convenient sensing of NH. Moreover, the rationally designed probe has many potential applications for imaging NH produced in situ during the metabolism of isoniazid in living cells based on the ratio of the fluorescent signal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124589DOI Listing
March 2021

Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injury.

Aging (Albany NY) 2020 12 3;13(1):1132-1152. Epub 2020 Dec 3.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, Guangdong, P.R. China.

Content and aims: Ginsenoside RG1 (RG1) is thought to enhance proliferation and differentiation of stem cell, however, its role on paracrine efficacy of stem cell remains unclear. Here we examined if and how RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) on radiation induced intestinal injury (RIII).

Method: Irradiated rats randomly received intraperitoneal injection of conditioned medium (CM) derived from non-activated BM-MSCs (MSC-CM) or BM-MSCs pre-activated by RG-1 (RG1-MSC-CM). Intestinal samples were collected, followed by the evaluation of histological and functional change, apoptosis, proliferation, inflammation, angiogenesis and stem cell regeneration. The effects of heme oxygenase-1 (HO-1) were investigated using HO-1 inhibitor or siRNA.

Result: RG1 enhanced the paracrine efficacy of BM-MSCs partially through upregulation of HO-1. RG1-MSC-CM rather than MSC-CM significantly improved the survival and intestinal damage of irradiated rats via improvement of intestinal proliferation/apoptosis, inflammation, angiogenesis and stem cell regeneration in a HO-1 dependent mechanism. The mechanism for the superior paracrine efficacy of RG1-MSC-CM is related to a higher release of two pivotal cytokines VEGF and IL-6.

Conclusion: Our study revealed that RG1 enhances paracrine effects of BM-MSCs on RIII, providing a novel method for maximizing the paracrine potential of MSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835034PMC
December 2020

[IL-17A/lL-17RA reduces cisplatin sensitivity of ovarian cancer SKOV3 cells by regulating autophagy].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Nov;40(11):1550-1556

Department of Gynecology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.

Objective: To investigate the effect of interleukin-17A (IL-17A) on chemosensitivity of ovarian cancer cells to cisplatin (DDP) and explore the mechanism in light of autophagy regulation.

Methods: Ovarian cancer SKOV3 cells cultured were treated with different concentrations of DDP (1-20 μg/mL). MTT assay was used to observe the changes in proliferation of the treated cells and the effect of treatment with 100 ng/mL IL-17A for 24 h on DDP-induced apoptosis of SKOV3 cells. We then examined the expression of IL-17A receptor (IL-17RA) in SKOV3 cells using flow cytometry. Annexin V-FITC/PI double staining was used to detect the cell apoptosis rate, and early apoptosis of the cells was detected with JC-1 assay. A neutralizing monoclonal antibody (mAb) against IL-17RA was used to block IL-17RA. We also observed the effects of IL-17RA silencing mediated by a siRNA targeting IL-17RA (siRNA-IL-17RA) and treatment with 3-methyladenine (3-MA) for inhibiting autophagy on DDP-induced apoptosis of SKOV3 cells. The expressions of apoptosis-related proteins (Bcl-2, Bax, and cleaved caspase-3) and autophagy-related proteins (P62 and Beclin-1) in the treated cells were detected using Western blotting.

Results: DDP increased the expression of IL-17RA in ovarian cancer SKOV3 cells. Treatment with IL-17A significantly reduced the susceptibility of SKOV3 cells to cisplatin-induced apoptosis ( < 0.05). DDP obviously augmented the expression of Beclin-1 and reduced the autophagy degradation substrate P62 protein in the cells ( < 0.05). IL-17A/IL-17RA strongly enhanced the DDPinducted autophagy of the cells ( < 0.05). Blocking autophagy with 3-MA significantly increased DDP- induced apoptosis of SKOV3 cells with IL-17RA silencing, lowered the expression of Bcl-2 and enhanced Bax expression in the cells ( < 0.05).

Conclusions: IL-17A/IL-17RA can decrease chemosensitivity of SKOV3 cells to DDP by upregulating DDP-induced autophagy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12122/j.issn.1673-4254.2020.11.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7704378PMC
November 2020

A synthetic chalcone derivative, compound 39, alleviates lipopolysaccharide-induced acute lung injury in mice.

Eur J Pharmacol 2021 Jan 11;891:173730. Epub 2020 Nov 11.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address:

Our research group has previously synthesized various chalcone analogues. Of these analogues, compound 39 has been shown to exhibit potent antioxidative activities but its anti-inflammatory and anti-apoptosis effects remain unclear. Thus, the present study investigated the in vivo and in vitro effects and mechanisms of compound 39 in lipopolysaccharide (LPS)-induced acute lung injury (ALI). To induce ALI, the mice received LPS via a tracheal instillation 6 h after intragastric administration of compound 39 or vehicle. Histological changes, the lung wet/dry weight ratio, and the amounts of protein and inflammatory cells in the broncho-alveolar lavage (BAL) fluid were assessed after 24 h. Additionally, to determine its underlying mechanisms, Western blot and immunofluorescence analyses were used. Moreover, the in vitro effects of compound 39 were also investigated. In the in vivo experiment, compound 39 markedly alleviated histopathological alterations, lung edema, and protein leakage, and exhibited potent anti-inflammatory effects. In the in vitro experiments, compound 39 dose-dependently reduced the levels of pro-inflammatory cytokines and reactive oxygen species. The results suggested that the anti-inflammatory effects of compound 39 were due to suppression of the mitogen-activated protein kinase (MAPK)/nuclear factor κB (NF-κB) pathway. Compound 39 also enhanced the protein levels of Bcl-2 and reduced the protein levels of Bax and cleaved caspase-3. The present study confirmed the anti-inflammatory, oxy-radical prohibitive, and anti-apoptosis activities of compound 39 against LPS-induced tissue and cell damage, and revealed the mechanisms underlying those processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejphar.2020.173730DOI Listing
January 2021