Publications by authors named "Beibei Chen"

273 Publications

Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats.

J Ethnopharmacol 2021 Jun 19;279:114348. Epub 2021 Jun 19.

Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, 100029, China. Electronic address:

Ethnopharmacological Relevance: Fructus Ligustri Lucidi (FLL) is an edible herb with anti-osteoporotic activity, yet whether and how the aqueous extract of this herb affect calcium metabolism in preservation of bone quality remain unclear.

Aim Of The Study: To investigate the effects of FLL aqueous extract on calcium balance and short-chain fatty acids (SCFAs) production in ovariectomized (OVX) rats.

Materials And Methods: OVX rats were daily and orally administrated with FLL aqueous extract (3.5 g/kg) for 14 weeks. The levels of N-terminal propeptide of type I collagen (PINP) and C-terminal telopeptide of type I collagen (CTx-I) in rat serum were evaluated by ELISA assays. The concentration of calcium in serum, urine, and feces were determined by biochemical assays. Bone quality was determined by Micro-CT, a three-point bending assay, and Fourier Transform Infrared (FTIR) Spectrometry. The expressions of Calbindin D28K and Calcium-sensing receptor (CaSR) in kidney as well as the Vitamin D receptor (VDR), the transient receptor potential vanilloid receptor 6 (TRPV6), Calbindin D9k in the duodenum were measured by immunohistochemistry, western blotting, or real-time PCR. The short-chain fatty acids (SCFAs) levels in the feces of the cecum were tested by gas chromatograghy.

Results: The administration of FLL to OVX rats resulted in a significant improvement in bone mineral density and biomechanical strength as well as in maintaining bone microstructures and material quality. Meanwhile, the decreased levels of PINP and increased levels of CTx-I in OVX rats were restored by FLL treatment. Additionally, FLL treatment increased calcium absorption, upregulated VDR, TRPV6, Calbindin D9k expressions in the duodenum, Calbindin D28K in kidney, and down-regulated CaSR expression in the kidney, as well as enhanced SCFAs levels in the feces of OVX rats.

Conclusions: FLL aqueous extract may preserve bone quality through regulation of the calcium balance and intestinal SCFAs production in OVX rats. This offers translational value of FLL into osteoporosis clinical trial.
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http://dx.doi.org/10.1016/j.jep.2021.114348DOI Listing
June 2021

The amino - functionalized magnetic graphene oxide combined with graphite furnace atomic absorption spectrometry for determination of trace inorganic arsenic species in water samples.

Talanta 2021 Sep 15;232:122425. Epub 2021 Apr 15.

Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.

A novel adsorbent of magnetic graphene oxide (GO) chemically modified by cysteamine hydrochloride ([email protected]/GO-NH) through thiol-ene click chemistry reaction was synthesized. The prepared [email protected]/GO-NH exhibit selective adsorption to As(V) with high adsorption capacity (52.66 mg g). Taking [email protected]/GO-NH as the adsorbents, a new method of magnetic solid phase extraction (MSPE) combined with graphite furnace atomic absorption spectrometry (GFAAS) was developed in determining trace-level inorganic arsenic species (As(III) and As(V)) in environmental water and bottled water samples. Various experimental parameters affecting the MSPE have been optimized. Under the optimal experimental parameters, the limit of detection of the established method for As(V) was 1.02 ng L, the relative standard deviations were 7.9% (intra-day, c = 50 ng L, n = 5) and 4.6% (inter-day, c = 50 ng L, n = 7), respectively, and the enrichment factor of the method was 392. GBW08666 and GBW08667 (certified reference material) were analyzed to confirm the accuracy of the method, and the results were matched well with the certified values. The established MSPE-GFAAS method was successfully applied in analyzing trace/ultratrace As(III) and As(V) in real water samples.
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http://dx.doi.org/10.1016/j.talanta.2021.122425DOI Listing
September 2021

Combined effects of different sizes of ZnO and ZIF-8 nanoparticles co-exposure with Cd on HepG2 cells.

Sci Total Environ 2021 Sep 1;786:147402. Epub 2021 May 1.

Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address:

Heavy metal and nanoparticles (NPs) emitted in the environment have attracted worldwide attention. But the combined effect of NPs and heavy metals is still unclear. In this study, the combined effect of zinc-based NPs and Cd on HepG2 cells was investigated by combining biological indicator detection methods with time-resolved inductively coupled plasma mass spectrometry (TRA-ICP-MS) single cell analysis, and the combined effect of Zn and Cd was also investigated for a comparison. High-dose of ZnO or ZIF-8 NPs co-exposure with Cd would reduce the cell viability while low-dose of ZnO or ZIF-8 NPs co-exposure with Cdshowed antagonism and the particle size has no remarkable effect on the combined toxicity. In the antagonism, Zn would increase cellular Zn amount through increasing the expression of ZIP8 and ZIP14 transporters to manage the ROS generation, but the zinc-based NPs would decrease expression of these transporters to decrease cellular Cd amount to help maintain the cell viability. Thus, we should hold a dialectical thinking about the pollution of NPs emissions in the environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147402DOI Listing
September 2021

Reduced graphene oxide coated nickel foam for stir bar sorptive extraction of benzotriazole ultraviolet absorbents from environmental water.

Talanta 2021 Aug 9;231:122332. Epub 2021 Apr 9.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.

The use of porous carrier and coating sorbents in stir bar sorptive extraction (SBSE) contributes to the improvement of extraction efficiency and dynamics. Herein, porous nickel foam (NF) with large surface area and magnetic property was used as the carrier of stir bar. NF was added into the mixture of graphene oxide (GO) and reducing agent, and reduced graphene oxide (RGO) coating was obtained on the surface of NF substrate by in situ hydrothermal reduction. The characterization results of Fourier transform infrared spectroscopy, X-ray power-diffraction and scanning electron microscope showed that GO was partially reduced into RGO, and the RGO coating was uniformly loaded on the NF surface. The obtained RGO-NF composite was used as the stir bar coating for the analysis of six benzotriazole (BZTs) UV absorbents. The extraction efficiency was between 48 and 64% for six BZTs. RGO-NF stir bar exhibited faster adsorption/desorption kinetics than commercial polydimethylsiloxane coated stir bar (50 min vs 120/360 min) due to its porous structure and large specific surface area. On this basis, a method of RGO-NF coated stir bar sorptive extraction combined with high performance liquid chromatography (HPLC)-DAD was established for the determination of six BZTs. Under the optimized conditions, the limits of detection were 0.33-0.50 μg/L for six target BZTs, and the linear range was 1-100 μg/L. The proposed method merits good ability to resist matrix and was used to analyze six BZTs in environmental water samples. The recoveries of target BZTs were obtained within 83.0-112% in the spiked East Lake water and 97.0-111% in the spiked Yangtze River water, respectively.
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http://dx.doi.org/10.1016/j.talanta.2021.122332DOI Listing
August 2021

Corrigendum: Expression of Human Epidermal Growth Factor Receptor-2 Status and Programmed Cell Death Protein-1 Ligand Is Associated With Prognosis in Gastric Cancer.

Front Oncol 2021 20;11:672599. Epub 2021 Apr 20.

Department of Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

[This corrects the article DOI: 10.3389/fonc.2020.580045.].
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http://dx.doi.org/10.3389/fonc.2021.672599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095244PMC
April 2021

Curcumin improves adipocytes browning and mitochondrial function in 3T3-L1 cells and obese rodent model.

R Soc Open Sci 2021 Mar 17;8(3):200974. Epub 2021 Mar 17.

Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.

Accumulating evidence suggests that mitochondrial dysfunction and adipocyte differentiation promote lipid accumulation in the development of obesity and diabetes. Curcumin is an active ingredient extracted from that has been shown to exhibit antioxidant and anti-inflammatory potency in metabolic disorders. However, the underlying mechanisms of curcumin in adipocytes remain largely unexplored. We studied the effects of curcumin on adipogenic differentiation and mitochondrial oxygen consumption and analysed the possible mechanisms. 3T3-L1 preadipocytes were used to assess the effect of curcumin on differentiation of adipocytes. The Mito Stress Test measured by Seahorse XF Analyzer was applied to investigate the effect of curcumin on mitochondrial oxygen consumption in 3T3-L1 adipocytes. The effect of curcumin on the morphology of both white and brown adipose tissue (WAT and BAT) was evaluated in a high-fat diet-induced obese mice model. We found that curcumin dose-dependently (10, 20 and 35 µM) induced adipogenic differentiation and the intracellular fat droplet accumulation. Additionally, 10 µM curcumin remarkably enhanced mature adipocyte mitochondrial respiratory function, specifically, accelerating basic mitochondrial respiration, ATP production and uncoupling capacity via the regulation of peroxisome proliferator-activated receptor γ (PPARγ) ( < 0.01). Curcumin administration also attenuated the morphological changes in adipose tissues in high-fat diet-induced obese mice. Moreover, curcumin markedly increased the mRNA and protein expressions of mitochondrial uncoupling protein 1 (UCP1), PPARγ, peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and PR domain protein 16 (PRDM16) and . Collectively, the results demonstrate that curcumin promotes the adipogenic differentiation of preadipocytes and mitochondrial oxygen consumption in 3T3-L1 mature adipocytes by regulating UCP1, PRDM16, PPARγ and PGC-1α expression.
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http://dx.doi.org/10.1098/rsos.200974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074937PMC
March 2021

Magnetic nanomaterials as sorbents for trace elements analysis in environmental and biological samples.

Talanta 2021 Aug 16;230:122306. Epub 2021 Mar 16.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

This review focuses on magnetic nanomaterials as sorbents for trace elements analysis in environmental and biological samples. The design and preparation of magnetic nanomaterials with specific functional groups for trace elemental analysis are summarized, along with relevant adsorption mechanism. The application of these magnetic sorbents in different operation modes for the quantification of trace elements and their species in environmental and biological samples are discussed. The trend of development in this field is also prospected.
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http://dx.doi.org/10.1016/j.talanta.2021.122306DOI Listing
August 2021

A dual-functional magnetic microsphere for ICP-MS quantification and fluorescence imaging of matrix metalloproteinase 2 in cell secretion.

Anal Chim Acta 2021 May 5;1161:338479. Epub 2021 Apr 5.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

Matrix metalloproteinase 2 (MMP2) plays an important role in tumor growth, invasion and metastasis. In this work, a dual-functional magnetic microsphere probe was designed for ICP-MS quantification and fluorescence imaging of MMP2 in cell secretion. In the designed probe, a NH-peptide (-FAM)-biotin was used as a bridge for the combination of carboxylated magnetic beads (MBs-COOH) and streptavidin functionalized gold nanoparticle (Au NP-SA). Initially, the fluorescence of FAM was quenched by Au NP. Since the NH-peptide (-FAM)-biotin had a MMP2-specifically recognized sequence, the peptide was specifically cleaved in the presence of MMP2, thus releasing Au NP for the ICP-MS quantification of MMP2 and turning on the fluorescence of FAM for the fluorescence imaging of MMP2. Under the optimal experimental conditions, a linear range of 0.05-50 ng mL and a limit of detection of 0.02 ng mL were obtained for MMP2. The relative standard deviation (n = 7, c = 0.1 ng mL) of the proposed method was 5.4%. With good sensitivity and good accuracy, the proposed method realized the quantification and imaging of MMP2 in A549 cell secretion. The proposed method was applied to monitor the expression of MMP2 in the A549 cell secretion under the stimulation of Cd, providing a new detection strategy in the study of MMP2-related life process.
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http://dx.doi.org/10.1016/j.aca.2021.338479DOI Listing
May 2021

Creativity and Leadership in the Creative Industry: A Study From the Perspective of Social Norms.

Front Psychol 2021 6;12:651817. Epub 2021 Apr 6.

School of Business, Dalian University of Technology, Dalian, China.

Individual creativity has been the focus of long-term research in creative industries. However, few studies have explored the impact on individual creativity from social factors. At the same time, the influence of individual creativity on the existence of subsequent factors in the creative industry is also worthy of further investigation. From a social standpoint, this research aims to explore how social norms affect individual creativity, and how individual creativity affects subsequent leadership. The present research takes creative entrepreneurs in creative enterprises as the research objects, and the structural equation model is used to analyze the data obtained from 202 valid questionnaires. Besides, the mediating effect of individual creativity between social norms and individual leadership is verified. The results show that social norms can effectively promote the generation of individual creativity that has a positive impact on both transactional or transformational leadership. It is revealed that social norms are effective tools for enhancing creativity, answering the question of how creative ideas are transformed into creative work and leadership. Individual creativity plays a mediating role between social norms and individual leadership.
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http://dx.doi.org/10.3389/fpsyg.2021.651817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057347PMC
April 2021

The role of miR-27b-3p/HOXA10 axis in the pathogenesis of endometriosis.

Ann Palliat Med 2021 Mar;10(3):3162-3170

Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, China.

Background: To explore the effects of microRNA (miR)-27b-3p-mediated homeobox A10 (HOXA10) on the proliferation, migration, and invasion of endometriosis cells (hEM15A).

Methods: First, quantitative polymerase chain reaction (qPCR) was performed for the measurement of miR-27b-3p and HOXA10 expression in hEM15A cells and human embryonic stem cells (hESC). Then, the targeted relationship of miR-27b-3p with HOXA10 was verified by conducting a dual-luciferase reporter experiment. Subsequently, qPCR and western blot were performed to determine the effect of miR-27b-3p on HOXA10 expression. Finally, Cell Counting Kit-8, Transwell, and scratch assays were employed to determine the effects of miR-27b-3p and HOXA10 on the proliferative, migratory, and invasive abilities of hEM15A cells.

Results: In hEM15A cells, miR-27b-3p expression was increased and showed a negative correlation with the expression of HOXA10 (P<0.05). The dual-luciferase reporter experiment confirmed that miR-27b-3p targeted the HOXA10 gene. Furthermore, qPCR and western blotting showed that miR-27b-3p regulated the expression of HOXA10. The proliferation, migration, and invasion abilities of hEM15A cells was significantly inhibited by suppressing miR-27b-3p expression or overexpressing HOXA10 (P<0.05). Meanwhile, concurrent overexpression of miR-27b-3p and HOXA10 did not affect hEM15A cell activity (P>0.05).

Conclusions: Upregulation of miR-27b-3p can suppress HOXA10 expression, resulting in the enhancement of hEM15A cell proliferation, migration, and invasion.
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http://dx.doi.org/10.21037/apm-21-343DOI Listing
March 2021

Carboxymethylation of polysaccharide isolated from Alkaline Peroxide Mechanical Pulping (APMP) waste liquor and its bioactivity.

Int J Biol Macromol 2021 Jun 23;181:211-220. Epub 2021 Mar 23.

College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address:

In recent years, the biological activity of polysaccharides and their derivatives has been widely studied. However, in addition to the natural polysaccharides directly extracted from plants and animals, there are rich polysaccharides in the pulping waste liquor that have not been fully utilized. The extracted polysaccharide from eucalyptus Alkaline Peroxide Mechanical Pulping (APMP) waste liquor was used as a raw material. For the production of carboxymethyl polysaccharide, the effects of temperature (T), the amount of alkali (NaOH) and the amount of etherifying agent (ClCHCOOH) on the degree of substitution (DS) were investigated, the optimal preparation conditions are: reaction time 2 h, temperature 75 °C, and the molar ratio of polysaccharide, NaOH and ClCH2COOH is 1:1:2, the highest DS is 1.47; FT-IR, NMR and GPC were used to characterize the structure and Molecular weight, the results show that the polysaccharide of APMP waste liquor is rich in xylan, and it was proved that the carboxymethyl substitution was successful and the positions of the substituent group were determined. The characterization and biological activity research of xylan polysaccharide (XP) and carboxymethyl xylan polysaccharide (CMXP), such as antioxidation, moisture absorption/retention, bacteriostatic action and cytotoxicity were discussed. CMXP shows better effects compared with XP.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.125DOI Listing
June 2021

A Homogeneous Multicomponent Nucleic Acid Enzyme Assay for Universal Nucleic Acid Detection by Single-Particle Inductively Coupled Plasma Mass Spectrometry.

Anal Chem 2021 03 9;93(11):4952-4959. Epub 2021 Mar 9.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.

Single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) has great potential for sensitive analysis of nucleic acids; however, it usually requires separation of target-induced nanoparticle reporters, and the sequence of probes on nanoparticle reporters has to be tuned for each target accordingly. Here, we developed a homogeneous multicomponent nucleic acid enzyme (MNAzyme) assay for universal nucleic acid detection. The two components of MNAzyme contain target recognition sites, substrate binding sites, and a catalytic core. Only in the presence of a specific nucleic acid target, the MNAzyme will assemble to trigger its nucleic acid enzyme activity and cleave its substrate (Linker DNA). The Linker DNA could link gold nanoparticle (AuNP) probes to form a larger assembled particle, while the cleavage of Linker DNA will disturb the linkage between probes, inducing a smaller assembled particle. The assembled particles with different sizes could be differentiated and sensitively detected in SP-ICP-MS, which also enables the tolerance of a complex matrix. By simply altering the sequences of the target recognition sites in MNAzyme, we applied the assay for two types of nucleic acids (long strand DNA and short strand RNA), malaria DNA and miRNA-10b. With increasing the target concentration, the signal intensity of each assembled particle decreases, but the frequency of assembled particle pulse increases. Both targets could be quantitatively detected from 0.1 to 25 pmol L with high specificity in serum samples. The developed MNAzyme-SP-ICP-MS assay possesses simple operation in a homogeneous reaction, easy tunability for multiple types of nucleic acid targets, and good compatibility with clinic samples.
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http://dx.doi.org/10.1021/acs.analchem.0c05444DOI Listing
March 2021

The efficacy and safety of apatinib treatment for patients with advanced or recurrent biliary tract cancer: a retrospective study.

BMC Cancer 2021 Feb 23;21(1):189. Epub 2021 Feb 23.

Department of Oncology, The Affiliated Cancer Hospital of Zhengzhou University, No. 127 Dongming Road, Jinshui District, Zhengzhou City, 450008, Henan Province, China.

Introduction: The present study aims to evaluate the efficacy and safety of apatinib monotherapy or combination therapy for patients with advanced or recurrent biliary tract cancer (BTC).

Methods: Twenty-eight patients with advanced or recurrent BTC who progressed after prior systemic therapies and treated with apatinib from January 2017 to June 2019 were enrolled in this retrospective and observational study. The primary end point was progression free survival (PFS). Secondary end points included overall survival (OS), objective response rate (ORR), disease control rate (DCR), and toxicity.

Results: A total of 28 patients with advanced or recurrent BTC who progressed after prior systemic therapies received apatinib monotherapy or combination therapy (with capecitabine, S-1, oxaliplatin, irinotecan or PD-1 inhibitor), including 9 cases of gallbladder cancer and 19 cases of cholangiocarcinoma. Six patients achieved PR, 15 patients had SD and 7 patients had PD. Median progression-free survival (PFS) and overall survival (OS) was 4.3 months (95%CI = 1.8-6.8) and 6.2 months (95% CI = 4.6-7.8) respectively. The ORR and DCR were 21.4% (6/28) and 75.0% (21/28), respectively. Most of the adverse events were grade 1-2 in severity, apatinib treatment was well tolerated.

Conclusions: Apatinib monotherapy or combination therapy can improve PFS in patients with advanced or recurrent BTC who progressed after prior systemic therapies, and adverse reactions can be well tolerated. Our study support apatinib therapy as a feasible therapeutic strategy in advanced or recurrent BTC.
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http://dx.doi.org/10.1186/s12885-021-07907-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903638PMC
February 2021

Controlled Synthesis and Surface Engineering of Janus Chitosan-Gold Nanoparticles for Photoacoustic Imaging-Guided Synergistic Gene/Photothermal Therapy.

Small 2021 Mar 23;17(11):e2006004. Epub 2021 Feb 23.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

The unsymmetrical morphology and unique properties of Janus nanoparticles (JNPs) provide superior performances for biomedical applications. In this work, a general and facile strategy is developed to construct a series of symmetrical and unsymmetrical chitosan/gold nanoparticles. Taking advantage of the active motion derived from Janus structure, selective surface functionalization of polysaccharide domain, and photothermal effect of gold nanorods, Janus chitosan/gold nanoparticles (J-Au-CS) are selected as a model system to construct Janus-structured chitosan/gold nanohybrids (J-ACP). Near-infrared (NIR)-responsive J-ACP composed of polycationic chitosan nanospheres and PEGylated gold nanorods hold great potential to realize photoacoustic (PA) imaging-guided complementary photothermal therapy (PTT)/gene therapy for breast cancer. The morphology effect of chitosan/gold nanostructures on enhanced PTT, cellular uptake, and gene transfection is investigated. The feasibility of PA imaging to track the accumulation of J-ACP and guide PTT is also explored. Notably, synergistic therapy is achieved based on PTT-enhanced gene therapy. In addition, the loading function of chitosan/gold nanoparticles for fluorescence imaging is demonstrated. The current work extends the application of JNPs for imaging-guided synergistic cancer therapy and provides flexible candidates with distinct structures for diverse biomedical applications.
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http://dx.doi.org/10.1002/smll.202006004DOI Listing
March 2021

Analysis of Main Components in Jujube and Mulberry Extracts by High-Sensitive HPLC-ESI-Q-TOF-MS/MS.

J Chromatogr Sci 2021 Feb 17. Epub 2021 Feb 17.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P R China.

In order to comprehensively reflect the cigarette quality, a method combining direct injection of diluted sample with high sensitive high-performance liquid chromatography (HPLC)- electrospray (ESI)- quadrupole (Q)- time of flight (TOF)- tandem mass spectrometry (MS/MS) was developed for the identification of major components of cigarette essences (jujube and mulberry extracts). Based on the observed relative molecular mass, MS/MS fragmentation behavior, MS/MS database and related literatures, the components of the jujube extract and mulberry extract were identified. The result shows that the composition of jujube extract and mulberry extract has some similarity. They all mainly contain amino acids, free amino compounds and Maillard reaction products, which are the main constituents of a cigarette essence.
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http://dx.doi.org/10.1093/chromsci/bmaa133DOI Listing
February 2021

Expression of Human Epidermal Growth Factor Receptor-2 Status and Programmed Cell Death Protein-1 Ligand Is Associated With Prognosis in Gastric Cancer.

Front Oncol 2020 1;10:580045. Epub 2021 Feb 1.

Department of Oncology, Henan Cancer Hospital, Zhengzhou, China.

Background: PD-L1 and HER-2 are routine biomarkers for gastric cancer (GC). However, little research has been done to investigate the correlation among PD-L1, HER-2, immune microenvironment, and clinical features in GC.

Methods: Between January 2013 and May 2020, a total of 120 GC patients treated with chemotherapy were admitted to Henan Tumor Hospital. We retrospectively identified PD-L1, HER-2 level before chemotherapy and abstracted clinicopathologic features and treatment outcomes. Univariate and multivariate survival analyses were performed to assess the relationship between PD-L1/HER-2 levels and progression-free survival (PFS). The mRNA and tumor microenvironment of 343 patients with GC from The Cancer Genome Atlas (TCGA) were used to explore the underlying mechanism.

Results: We retrospectively analyzed 120 patients with gastric cancer, including 17 patients with HER-2 positive and 103 patients with HER-2 negative GC. The results showed that the expression of PD-L1 was closely correlated with HER-2 (P = 0.015). Patients with PD-L1/HER-2 positive obtained lower PFS compared to PD-L1/HER-2 negative (mPFS: 6.4 . 11.1 months, P = 0.014, mPFS: 5.3 . 11.1 months, P = 0.002, respectively), and the PD-L1 negative and HER-2 negative had the best PFS than other groups (P = 0.0008). In a multivariate model, PD-L1 status, HER-2 status, tumor location, and tumor differentiation remained independent prognostic indicators for PFS (P < 0.05). The results of database further analysis showed that the proportion of PD-L1+/CD8A+ in HER-2 negative patients was higher than that in HER-2 positive patients (37.6 20.3%), while PD-L1-/CD8A- was significantly higher in HER-2 positive patients than HER-2 negative patients (57.8 . 28.8%). In addition, it showed that not only CD4+T cells, macrophages, and CD8+T cells, but also the associated inflammatory pathways such as IFN-/STAT1 were associated with HER-2.

Conclusion: HER-2 status could predict the efficacy of immune checkpoint inhibitors, and HER-2 status combined with PD-L1 level could predict the prognosis of GC patients.
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http://dx.doi.org/10.3389/fonc.2020.580045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882725PMC
February 2021

Composition of Intracellular Protein Corona around Nanoparticles during Internalization.

ACS Nano 2021 02 11;15(2):3108-3122. Epub 2021 Feb 11.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.

It has been well established that the early-stage interactions of nanoparticles with cells are governed by the extracellular protein corona. However, after entering into the cells, the evolving protein corona is the key to subsequent processing of nanoparticles by cells. To identify the protein corona around intracellular nanoparticles, it is essential to maintain its original compositions during cell treatment. Herein, we develop a paraformaldehyde (PFA) cross-linking strategy to stabilize corona compositions when extracting protein coronas from cells, providing original information on protein coronas around intercellular gold nanoparticles (AuNPs). The stability of the protein corona after PFA cross-linking was carefully investigated with several characterization methods, and the results demonstrate that PFA cross-linking successfully prevents the dissociation and exchange of corona proteins. Then the recovered intracellular protein corona around AuNPs from living HepG2 cells with a PFA cross-linking strategy was subjected to nanoHPLC-MS/MS for proteomic analysis. It was found that the compositions of intracellular protein coronas are dominated by cell-derived proteins and undergo significant variation of protein species and amounts over time during internalization. Time-resolved analysis provides relevant proteins involved in nanoparticle cellular uptake and transportation, indicating that AuNPs are endocytosed mainly by a clathrin-mediated uptake mechanism and directed into an endolysosomal pathway toward their final destination. Such proteomic-based results are verified by pharmacological inhibition and TEM imaging analysis. This work provides a universal strategy to study compositions of protein corona around intercellular nanoparticles and could be a footstone to link the formation of protein corona around nanoparticles to their biological function in cells.
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http://dx.doi.org/10.1021/acsnano.0c09649DOI Listing
February 2021

BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-κB pathways in aging mice induced by D-galactose and NaNO.

J Ethnopharmacol 2021 May 26;271:113810. Epub 2021 Jan 26.

Department of Chinese Medicine, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510630, China. Electronic address:

Ethnopharmacological Relevance: Sperm infertility and testicular atrophy are symptoms associated with aging. BaZiBuShen formula (BZBS), a patented Chinese herbal prescription composed of Semen Cuscutae, Fructus Lycii, Epimedii Folium, Fructus Schisandrae Sphenantherae, Fructus Cnidii, Fructus Rosae Laevigatae, Semen Allii Tuberosi., Radix Morindae Officinalis, Herba Cistanches, Fructus Rubi, Radix Rehmanniae Recens, Radix Cyathulae, Radix Ginseng, Cervi Cornu Pantotrichum, Hippocampus, and Fuctus Toosendan, has been used as a kidney-tonifying and anti-aging drug as well as for the treatment of impotence and male infertility in traditional Chinese medicine.

Aim Of The Study: We aimed at investigating whether BZBS preserves sperm and testes morphology in aging mice, and to explore the underlying mechanisms.

Materials And Methods: BZBS was orally administered to aging mice induced by D-galactose (D-gal) and NaNO for 65 days. Sperm quality and testes pathophysiological alterations were examined by a Semen Analysis System, hematoxylin-eosin staining, transmission electron microscopy, and mitochondrial complex IV activity. In addition, serum levels of total antioxidant capacity (TAC), malondialdehyde (MDA), 8-hydroxy-desoxyguanosine (8-OH-dG), reduced glutathione (GSH), oxidized glutathione disulfide (GSSG), testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E) and tumor necrosis factor-α (TNF-α) were determined by ELISA. The expressions of P450 aromatase (CYP19), sirtuin 6 (Sirt6), P53, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-κB)-p65, and phospho-NF-κB-p65 (NF-κB-pp65) in the testes were examined by western blot and/or immunohistochemical staining.

Results: Sustained exposure to D-gal/NaNO caused a deterioration of sperm quality and testes morphology in this rapid aging mouse model. BZBS treatment curtailed these alterations. These beneficial effects were associated with increased serum levels of TAC, GSH/GSSG, T, E, and FSH, and decreased levels of MDA, TNF-α, and 8-OH-dG. BZBS treatment also downregulated the expressions of P53, iNOS, and NF-κB-pp65, as well as upregulated the expressions of Sirt6 and CYP19 in aging testes.

Conclusions: BZBS preserves testicular morphology and spermatogenesis possibly via inhibition of oxidative stress and the modulation of the Sirt6/P53 and Sirt6/NF-κB signaling pathways. The results shed light on the beneficial effect of BZBS on sperm quality and fertility in aging males.
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http://dx.doi.org/10.1016/j.jep.2021.113810DOI Listing
May 2021

Research on the Application of Blockchain in Smart Healthcare: Constructing a Hierarchical Framework.

J Healthc Eng 2021 12;2021:6698122. Epub 2021 Jan 12.

Graduate School of Business, Changwon National University, Changwon 51140, Republic of Korea.

This study aims to explore the application of blockchain technology in smart healthcare, establish a hierarchical theoretical framework of smart healthcare, reveal the impact of blockchain on smart healthcare, and finally, construct a development application system of smart healthcare under the blockchain based on stakeholder theory. However, such a hierarchical theoretical framework should consider not only the necessary attributes and the interrelationship among various aspects and attributes but also the role of multiple stakeholders. Therefore, the paper uses fuzzy set theory to filter unnecessary attributes, proposes a decision-making and experimental evaluation laboratory (DEMATEL) to manage the complex interrelationships between various aspects and attributes, and uses Interpretive Structure Modeling (ISM) to divide the hierarchy and construct a hierarchical theoretical framework. The results show that (1) the top-level design, the medical record management, and the doctor management are the root causes of system. (2) The specific application of blockchain in the field of smart healthcare is mainly carried out around the intelligent contract, which relies on the medical record management and is constrained by the system, and optimization of application is the key to system upgrading. (3) The internal and external regulation, the medical insurance, and the environmental governance play a guaranteed role for the development of the system and effectively safeguard the interests of stakeholders. (4) The application system of smart healthcare under the blockchain needs to be built based on three layers: the transaction layer, information layer, and stakeholder layer. The theoretical hierarchical framework is intended to guide smart healthcare towards blockchain applications, and stakeholders are suggested to participate in the development application systems.
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http://dx.doi.org/10.1155/2021/6698122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815389PMC
January 2021

Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin.

Proc Natl Acad Sci U S A 2021 02;118(5)

Center for Autophagy Research, University of Texas Southwestern Medical Center, Dallas, TX 75390.

Beclin 1, an autophagy and haploinsufficient tumor-suppressor protein, is frequently monoallelically deleted in breast and ovarian cancers. However, the precise mechanisms by which Beclin 1 inhibits tumor growth remain largely unknown. To address this question, we performed a genome-wide CRISPR/Cas9 screen in MCF7 breast cancer cells to identify genes whose loss of function reverse Beclin 1-dependent inhibition of cellular proliferation. Small guide RNAs targeting and , tumor-suppressor genes that encode cadherin/catenin complex members E-cadherin and alpha-catenin, respectively, were highly enriched in the screen. CRISPR/Cas9-mediated knockout of or reversed Beclin 1-dependent suppression of breast cancer cell proliferation and anchorage-independent growth. Moreover, deletion of or inhibited the tumor-suppressor effects of Beclin 1 in breast cancer xenografts. Enforced Beclin 1 expression in MCF7 cells and tumor xenografts increased cell surface localization of E-cadherin and decreased expression of mesenchymal markers and beta-catenin/Wnt target genes. Furthermore, CRISPR/Cas9-mediated knockout of and the autophagy class III phosphatidylinositol kinase complex 2 (PI3KC3-C2) gene, , but not PI3KC3-C1-specific or other autophagy genes , , or , resulted in decreased E-cadherin plasma membrane and increased cytoplasmic E-cadherin localization. Taken together, these data reveal previously unrecognized cooperation between Beclin 1 and E-cadherin-mediated tumor suppression in breast cancer cells.
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http://dx.doi.org/10.1073/pnas.2020478118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865132PMC
February 2021

Knockdown of Kremen2 Inhibits Tumor Growth and Migration in Gastric Cancer.

Front Oncol 2020 7;10:534095. Epub 2021 Jan 7.

Department of Medical Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/-catenin signaling pathway. However, the role of Krm2 in gastric cancer is not clear. The aim of this study was to explore the regulatory role of Krm2 in the tumorigenesis and metastasis of gastric cancer. It was demonstrated that, compared to para-cancerous tissues, Krm2 was significantly up-regulated in gastric cancer tissues and was positively correlated with the pathological grade of gastric cancer patients. Given that Krm2 is abundantly expressed in most tested gastric cancer cell lines, Krm2 knockdown cell models were established and further used to construct mice xenograft model. After knocking down Krm2, both the cell survival and tumorigenesis of gastric cancer cells were inhibited. At the same time, knockdown of Krm2 induced apoptosis, cell cycle arrest at G/M phase and repression of migration in gastric cancer cells . Mechanistically, we found that knockdown of Krm2 suppressed PI3K/Akt pathway. Therefore, we revealed the novel role and the molecular mechanism of Krm2 in promoting the tumorigenesis and metastasis in gastric cancer. Krm2 can be a potent candidate for designing of targeted therapy.
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http://dx.doi.org/10.3389/fonc.2020.534095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817645PMC
January 2021

Stir bar sorptive extraction and its application.

J Chromatogr A 2021 Jan 15;1637:461810. Epub 2020 Dec 15.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address:

Recent progress of stir bar sorptive extraction (SBSE) in the past six years is reviewed. The preparation methods including electrodeposition, self-assembly, solvent exchange, physical magnetic adsorption and electrostatic spinning, for the coated stir bar are summarized and compared, specifically for a specific material for coatings fabrication, e.g., carbon-based materials and metal organic frameworks. The emerging materials (e.g., graphene, graphene oxide, carbon nanotubes, monolith, metal-organic frameworks and porous organic polymers) applied for coated stir bar fabrication are one of the focus of this review, along with their respective advantages in extraction process and application in trace analysis. The development and application of extraction apparatus of SBSE are also involved. Based on these information, the development status and prospects of SBSE as an efficient sample pretreatment technique in real sample analysis are discussed.
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http://dx.doi.org/10.1016/j.chroma.2020.461810DOI Listing
January 2021

Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice.

Int J Biol Macromol 2021 Feb 19;170:33-41. Epub 2020 Dec 19.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China. Electronic address:

Fast green FCF (FGF) is often used in foods, pharmaceuticals, and cosmetics. However, little is known about the interactions of FGF with amyloid-β protein (Aβ) associated with Alzheimer's disease. In this study, the inhibitory effects of FGF on Aβ fibrillogenesis, the disruption of preformed Aβ fibrils, the reduction of Aβ-induced cytotoxicity, and the attenuation of Aβ-induced learning and memory impairments in mice were investigated. FGF significantly inhibited Aβ fibrillogenesis and disintegrated the mature fibrils as evidenced by thioflavin T fluorescence and atomic force microscopy studies. Co-incubation of Aβ with FGF greatly reduced Aβ-induced cytotoxicity in vitro. Moreover, FGF showed a protective effect against cognitive impairment in Aβ-treated mice. Molecular dynamics simulations further showed that FGF could synergistically interact with the Aβ17-42 pentamer via electrostatic interactions, hydrogen bonds and π-π interactions, which reduced the β-sheet content, and disordered random coils and bend structures of the Aβ17-42 pentamer. This study offers a comprehensive understanding of the inhibitory effects of FGF against Aβ neurotoxicity, which is critical for the search of effective food additives that can combat amyloid-associated disease.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.12.115DOI Listing
February 2021

A Study of hTERT Promoter Methylation in Circulating Tumour DNAs of Patients with Ovarian Magnificent Tumour.

Onco Targets Ther 2020 1;13:12317-12323. Epub 2020 Dec 1.

Department of Gynecology, Hangzhou Women's Hospital, Hangzhou 310008, People's Republic of China.

Objective: Human telomerase reverse transcriptase (hTERT), a crucial enzyme for telomere maintenance, has been associated with the development of ovarian cancer (OC). The purpose of this study was to investigate the difference of methylation rates of hTERT promoter in tumour tissues and plasma samples of patients with ovarian magnificent tumour and those with ovarian benign tumour, as well as in plasma samples of healthy women. This study further aimed to establish a possible association between increased methylation rate of hTERT promoter and circulating tumour DNAs (ctDNA) amongst patients with ovarian magnificent tumour.

Methods: Tumour tissue samples and plasma samples were separately obtained from 17 patients with ovarian magnificent tumour (experiment group, group A) and from 15 patients with ovarian benign tumour (control group, group B). Another 15 plasma samples were acquired from healthy women (control group, group C). Promoter methylation was assessed by methylation-specific PCR (MSP). Statistical analysis was conducted using SPSS 22.0.

Results: Methylation of hTERT was observed in 76.5% of tumour tissue samples and in 70.6% of plasma samples from patients with ovarian magnificent tumour. It was also observed in 26.7% of tumour tissue samples and 20% of plasma samples from patients with ovarian benign tumour, and in 13.3% of plasma samples from healthy women. Comparing between plasmas and tissues, the respective rates of consistency, sensitivity and specificity were 70.59%, 76.9% and 50% in group A, and 80%, 50% and 90.9% in group B. Hence, the associations of hTERT methylation with ctDNAs (p=0.001) and tumour tissue samples (p=0.012) amongst patients with ovarian magnificent tumour were established.

Conclusion: An increased methylation of hTERT promoter is related to ctDNAs and tumour tissues of patients with ovarian magnificent tumour.
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http://dx.doi.org/10.2147/OTT.S274743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719343PMC
December 2020

Correction to: MicroRNA-138-5p targets the NFIB-Snail1 axis to inhibit colorectal cancer cell migration and chemoresistance.

Cancer Cell Int 2020 Nov 3;20(1):533. Epub 2020 Nov 3.

Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou University, 127 Dong Ming Road, Zhengzhou, 450008, Henan, People's Republic of China.

An amendment to this paper has been published and can be accessed via the original article.
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http://dx.doi.org/10.1186/s12935-020-01629-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607649PMC
November 2020

Aerobic, resistance and combined training for adults with chronic kidney disease: A protocol for a systematic review and network meta-analysis.

Medicine (Baltimore) 2020 Dec;99(49):e23518

Department of Nephrology, the Second Hospital.

Background: Chronic kidney disease (CKD) as a disease that poses a great threat to human health, which has become a public health issue of great concern. Studies have found that exercise training has a positive effect on improving the condition of chronic kidney disease. We will conduct a network meta-analysis to assess the effects of aerobic training, resistance training and combined aerobic and resistance training in treating CKD patients.

Methods: We will search PubMed, EMBASE, Medline, Cochrane Central Register of Controlled Trials (CENTRAL), and Web of science to identify randomized control trails (RCTs) that assessed the effect of different exercise training for CKD patients. Cochrane Handbook will be used to evaluate the risk of bias of included articles. We will use Stata or R software to perform data analysis.

Results And Conclusion: Our systematic review and network meta-analysis will be the first study that investigates the effect of different exercise training for CKD patients, and will provide evidence for management of chronic kidney disease.

Ethics And Dissemination: The data involved in this study are from published articles. For this reason, there is no need for ethical approval or patient consent.

Trial Registration: the registration number was: CRD42020157280.
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http://dx.doi.org/10.1097/MD.0000000000023518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717791PMC
December 2020

MNAzyme-Catalyzed Amplification Assay with Lanthanide Tags for the Simultaneous Detection of Multiple microRNAs by Inductively Coupled Plasma-Mass Spectrometry.

Anal Chem 2021 01 7;93(2):737-744. Epub 2020 Dec 7.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.

Quantification of multiple disease-related microRNAs (miRNAs) is of great significance for clinical diagnosis. Based on the simultaneous multiple element detection ability of inductively coupled plasma-mass spectrometry (ICP-MS) and good specificity of multicomponent nucleic acid enzymes (MNAzymes), a novel and simple method based on the MNAzyme amplification strategy and lanthanide labeling coupled with ICP-MS detection was proposed for the sensitive and simultaneous detection of three miRNAs (miRNA-21, miRNA-155, and miRNA-10b). Specifically, a probe consisting of streptavidin-modified magnetic beads (SA-MBs) and three DNA substrates labeled with lanthanide tags (Tb/Ho/Lu) was constructed. In the presence of target miRNAs, three pairs of MNAzymes were assembled where each pair was hybridized with the corresponding miRNA, and then the substrates on the SA-MBs were cleaved by the activated MNAzymes, continuously releasing the fragment with lanthanide tags. The released lanthanide tags in the supernatant were collected after magnetic separation and analyzed by ICP-MS, realizing the simultaneous quantification of multiple miRNAs. The correlation of the lanthanide tag signal with the miRNA concentration fitted well in a linear model in the range of 50-1000 pmol L (miRNA-21) and 50-2000 pmol L (miRNA-155 and miRNA-10b). The limits of detection for three miRNAs were 11-20 pmol L, with the relative standard deviations of 2.2-2.7%. The recoveries of target miRNAs in the human serum and HepG-2 cells were in the range of 87.2-111% and 93.3-111%, respectively. Overall, the method is ideal for the simultaneous quantification of multiple miRNAs with advantages of low spectral interference, high sensitivity, good selectivity, and strong resistance to the complex matrix.
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http://dx.doi.org/10.1021/acs.analchem.0c02455DOI Listing
January 2021

High soil pH enhances the network interactions among bacterial and archaeal microbiota in alpine grasslands of the Tibetan Plateau.

Environ Microbiol 2021 01 28;23(1):464-477. Epub 2020 Nov 28.

School of Life Sciences, Lanzhou University, Lanzhou, China.

Soil functions and processes are driven by complex microbial interactions. It is, therefore, critical to understand the coexistence patterns of soil microbiota, especially in fragile alpine ecosystems. We identified biogeographic patterns in the network-level topological features of the soil microbial co-occurrence network in the Tibetan alpine grasslands, based on high-throughput sequencing. We verified that soil pH was the most important environmental variable for predicting network-level topological features of soil microbial co-occurrence networks. Associations among soil microbiota were enhanced with increasing pH (5.17-8.92), and the network was the most stable at neutral pH. Moreover, node-level topological features suggested that the archaeal operational taxonomic units, compared with bacterial operational taxonomic units, hold a central role in the co-occurrence network. Network-level topological features revealed closer connections among soil microbiota in the steppe ecosystem than in the meadow ecosystem. Therefore, our study demonstrated that soil pH served as a critical environmental filter that influenced the potential associations and ecological signature of soil microbiota in the Tibetan alpine grasslands. These findings provide a new perspective on the distinct biogeographic patterns of co-occurrence networks, to explore the ecological role of soil microbiota and thus help manage soil bacterial and archaeal communities for provisioning alpine ecosystem services.
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http://dx.doi.org/10.1111/1462-2920.15333DOI Listing
January 2021

Sustainable method towards magnetic ordered mesoporous polymers for efficient Methylene Blue removal.

J Environ Sci (China) 2021 Jan 6;99:168-174. Epub 2020 Jul 6.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address:

The difficulty in achieving high removal efficiency for contaminants in textile wastewater over a wide range of pH impedes the progress of its treatment technique greatly. Herein, a facile and sustainable strategy was adopted for constructing magnetic ordered mesoporous polymers (M-OMPs) without the assistance of organic solvent and catalyst. The prepared M-OMPs were endowed with high special surface area and good superparamagnetism simultaneously, and exhibited high removal efficiency (>99%) for Methylene Blue (MB) within a short time (10 min) at a concentration of 50 mg/L. What's more, high removal efficiency was achieved over a wide range of pH 2-12 and the adsorption capacity for MB on M-OMPs was substantially retained even after 5 adsorption-desorption cycles, further demonstrating the application potential of M-OMPs in the decontamination of textile wastewater.
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http://dx.doi.org/10.1016/j.jes.2020.06.018DOI Listing
January 2021

Recent advances in single-cell analysis by inductively coupled plasma-mass spectrometry: A review.

Anal Chim Acta 2020 Nov 24;1137:191-207. Epub 2020 Jul 24.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China. Electronic address:

The content, migration and transformation of trace elements in living organisms play an important role in physiological activities. The analysis of trace elements in cells is of great significance to understand the function mechanism of trace elements in cells and organisms. Compared with conventional bulk analysis, many medical and clinical researches benefit from cell heterogeneity via single-cell analysis. Time-resolved analysis-inductively coupled plasma-mass spectrometry (TRA-ICP-MS) based strategies have the potential for both counting and quantitative assessment. It has been used to evaluate cell-to-cell difference in elemental content, cellular uptake of metal-containing drugs and nanoparticles. This review focuses on TRA-ICP-MS based methodologies for single-cell analysis over the past few decades. Recent advances in ICP-MS instrumentations (cell introduction systems and mass spectrometers), microfluidic platforms and their in-depth applications in single-cell analysis are summarized. This review also prospects future developments of TRA-ICP-MS for single-cell analysis.
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http://dx.doi.org/10.1016/j.aca.2020.07.041DOI Listing
November 2020