Publications by authors named "Bei Jiang"

185 Publications

Efficacy and Safety of Leflunomide for Refractory COVID-19: A Pilot Study.

Front Pharmacol 2021 2;12:581833. Epub 2021 Jul 2.

Department of Nephropathy, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may persist in patients with coronavirus disease 2019 (COVID-19) despite receiving standard care. In this pilot study of hospitalized adult patients (≥18 years of age), with radiologically confirmed pneumonia who were SARS-CoV-2 positive for more than 28 days despite standard care, were assigned to receive standard of care (SOC, grp I) or leflunomide + SOC (grp 2). After 2 weeks, grp 1 and grp 2 patients who continued to be SARS-CoV-2-positive received leflunomide for 14 days while continuing SOC. The primary outcomes were the rate of and time to SARS-CoV-2 clearance and the 14-day and 30-day hospital discharge rate. 12 patients were enrolled in grp 1 and 15 patients were in grp 2. The 14 days SARS-CoV-2 viral clearance rate was 80.0% (12/15) for grp 2 patients receiving leflunomide 16.7% for grp 1 patients (2/12) ( = 0.002). By day 14, the median time to SARS-CoV-2 clearance was 6.0 days (range 1-12, IQR 1-12) for grp 2 patients. In grp 1, two patients converted to viral negative on days 1 and 6 ( 0.002). The 14-day discharge rate was 73.3% (11/15) for the grp 2 vs. 8.3% (1/12) for grp 1 ( 0.001). The 30 days discharge rate was 100% (15/15) for the grp 2 vs. 66.7% (8/12) for grp 1. No severe adverse events or deaths were reported. Leflunomide may improve the SARS-CoV-2 clearance rate and discharge rate in patients with refractory COVID-19. The tolerability of the 14-28 days course of treatment with leflunomide is acceptable. These preliminary observations need to be verified by a large sample size and randomized controlled trial.
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http://dx.doi.org/10.3389/fphar.2021.581833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284962PMC
July 2021

Optoelectronic Artificial Synapses Based on Two-Dimensional Transitional-Metal Trichalcogenide.

ACS Appl Mater Interfaces 2021 Jul 25;13(26):30797-30805. Epub 2021 Jun 25.

Faculty of Physics and Electronic Science, Hubei Key Laboratory of Ferro- & Piezoelectric Materials and Devices, Hubei University, Wuhan 430062, P.R. China.

The memristor is a foundational device for an artificial synapse, which is essential to realize next-generation neuromorphic computing. Herein, an optoelectronic memristor based on a two-dimensional (2D) transitional-metal trichalcogenide (TMTC) is designed and demonstrated. Owing to the excellent optical and electrical characteristics of titanium trisulfide (TiS), the memristor exhibits stable bipolar resistance switching (RS) as a result of the controllable formation and rupturing of the conductive aluminum filaments. Multilevel storage is realized with light of multiple wavelengths between 400 and 808 nm, and the synaptic properties such as conduction modulation and spiking timing-dependent plasticity (STDP) are achieved. On the basis of the photonic potentiation and electrical habitual ability, Pavlovian-associative learning is successfully established on this TiS-based artificial synapse. All these results reveal the large potential of 2D TMTCs in artificial neuromorphic chips.
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http://dx.doi.org/10.1021/acsami.1c03202DOI Listing
July 2021

Rapid Repurposing of Novel Combination Drugs for the Treatment of Heart Failure via a Computationally Guided Network Screening Approach.

J Chem Inf Model 2021 Jun 20. Epub 2021 Jun 20.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.

Combination drugs, characterized by high efficacy and few side effects, have received extensive attention from pharmaceutical companies and researchers for the treatment of complex diseases such as heart failure (HF). Traditional combination drug discovery depends on large-scale high-throughput experimental approaches that are time-consuming and costly. Herein we developed a novel, rapid, and potentially universal computer-guided combination drug-network-screening approach based on a set of databases and web services that are easy for individuals to obtain and operate, and we discovered for the first time that the menthol-allethrin combination screened by this approach exhibited a significant synergistic cardioprotective effect . Further mechanistic studies indicated that allethrin and menthol could synergistically block calcium channels. Allethrin bound to the central cavity of the voltage-dependent L-type calcium channel subunit alpha-1S (CACNA1S) lead to a conformational change in an allosteric site of CACNA1S, thereby enhancing the binding of menthol to this allosteric site. In summary, we reported a potentially universal computational approach to combination drug screening that has been used to discover a new combination of menthol-allethrin against HF , providing a new synergistic mechanism and prospective agent for HF treatment.
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http://dx.doi.org/10.1021/acs.jcim.1c00132DOI Listing
June 2021

First-generation species-selective chemical probes for fluorescence imaging of human senescence-associated β-galactosidase.

Chem Sci 2020 Jun 17;11(28):7292-7301. Epub 2020 Jun 17.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology Shanghai 200237 China

Human senescence-associated β-galactosidase (SA-β-gal), the most widely used biomarker of aging, is a valuable tool for assessing the extent of cell 'healthy aging' and potentially predicting the health life span of an individual. Human SA-β-gal is an endogenous lysosomal enzyme expressed from , the catalytic domain of which is very different from that of β-gal, a bacterial enzyme encoded by . However, existing chemical probes for this marker still lack the ability to distinguish human SA-β-gal from β-gal of other species, such as bacterial β-gal, which can yield false positive signals. Here, we show a molecular design strategy to construct fluorescent probes with the above ability with the aid of structure-based steric hindrance adjustment catering to different enzyme pockets. The resulting probes normally work as traditional SA-β-gal probes, but they are unique in their powerful ability to distinguish human SA-β-gal from β-gal, thus achieving species-selective visualization of human SA-β-gal for the first time. NIR-emitting fluorescent probe as their representative further displays excellent species-selective recognition performance in biological systems, which has been herein verified by testing in senescent cells, in -transfected cells and in -β-gal-contaminated tissue sections of mice. Because of our probes, it was also discovered that SA-β-gal content in mice increased gradually with age and SA-β-gal accumulated most in the kidneys among the main organs of naturally aging mice, suggesting that the kidneys are the organs with the most severe aging during natural aging.
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http://dx.doi.org/10.1039/d0sc01234cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159415PMC
June 2020

Cross-sectional analysis of peripheral blood mononuclear cells in lymphopenic and non-lymphopenic relapsing-remitting multiple sclerosis patients treated with dimethyl fumarate.

Mult Scler Relat Disord 2021 Jul 7;52:103003. Epub 2021 May 7.

Division of Neurology, Department of Medicine, University of Alberta, Alberta Canada T6G 2M8, Edmonton, Canada. Electronic address:

Background: Relapsing-remitting multiple sclerosis (RRMS) is an autoimmune disorder of the central nervous system. Dimethyl Fumarate is a disease-modifying medication used to treat RRMS patients that can induce lymphopenia. We aimed to immunophenotype peripheral blood mononuclear cells (PBMC) in RRMS patients cross-sectionally and examine the characteristics and modifications of lymphopenia over time.

Methods: Characterization of PBMC was done by multiparametric flow cytometry. Patients had been on treatment for up to 4 years and were grouped into lymphopenic (DMF-L) and non-lymphopenic (DMF-N) patients.

Results: Lymphopenia affected the cell population changes over time, with other patient characteristics (gender, age, and previous treatment status) also having significant effects. In both lymphopenic and non-lymphopenic patients, PBMC percentages were reduced over time. While overall T and B cells frequencies were not affected, males, older patients and untreated patients had significant changes in B cell subpopulations over time. CD4 to CD8T cell ratio increased significantly in lymphopenic patients over time. CD4CD8T cell population was similarly reduced in both lymphopenic and non-lymphopenic patients, over time. While the monocyte and NK overall populations were not changed, non-classical monocyte subpopulation decreased over time in lymphopenic patients. We also found CD56CD16 and CD56CD16 NK cells frequencies changed over time in lymphopenic patients. Immune populations showed correlations with clinical outcomes measured by EDSS and relapse rate. Analysis of the overall immunophenotype showed that, while groups divided by other patient characteristics showed differences, the lymphopenia status overrode these differences, resulting in similar immunophenotype within DMF-L.

Conclusions: Our data provide evidence that under the same therapy, lymphopenia affects how the immunophenotype changes over time and can override the differences associated with other patient characteristics and possibly mask other significant changes in the immune profile of patients.
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http://dx.doi.org/10.1016/j.msard.2021.103003DOI Listing
July 2021

Controllable Synthesis of Wafer-Scale Graphene Films: Challenges, Status, and Perspectives.

Small 2021 Jun 9:e2008017. Epub 2021 Jun 9.

Center for Nanochemistry (CNC), Beijing Science and Engineering Center for Nanocarbons, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, P. R. China.

The availability of high-quality, large-scale, and single-crystal wafer-scale graphene films is fundamental for key device applications in the field of electronics, optics, and sensors. Synthesis determines the future: unleashing the full potentials of such emerging materials relies heavily upon their tailored synthesis in a scalable fashion, which is by no means an easy task to date. This review covers the state-of-the-art progress in the synthesis of wafer-scale graphene films by virtue of chemical vapor deposition (CVD), with a focus on main challenges and present status. Particularly, prevailing synthetic strategies are highlighted on a basis of the discussion in the reaction kinetics and gas-phase dynamics during CVD process. Perspectives with respect to key opportunities and promising research directions are proposed to guide the future development of wafer-scale graphene films.
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http://dx.doi.org/10.1002/smll.202008017DOI Listing
June 2021

Mesenchymal Stem Cells in Preclinical Infertility Cytotherapy: A Retrospective Review.

Stem Cells Int 2021 17;2021:8882368. Epub 2021 May 17.

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.

Infertility is a global reproductive disorder which is caused by a variety of complex diseases. Infertility affects the individual, family, and community through physical, psychological, social and economic consequences. The results from recent preclinical studies regarding stem cell-based therapies are promising. Stem cell-based therapies cast a new hope for infertility treatment as a replacement or regeneration strategy. The main features and application prospects of mesenchymal stem cells in the future of infertility should be understood by clinicians. Mesenchymal stem cells (MSCs) are multipotent stem cells with abundant source, active proliferation, and multidirectional differentiation potential. MSCs play a role through cell homing, secretion of active factors, and participation in immune regulation. Another advantage is that, compared with embryonic stem cells, there are fewer ethical factors involved in the application of MSCs. However, a number of questions remain to be answered prior to safe and effective clinical application. In this review, we summarized the recent status of MSCs in the application of the diseases related to or may cause to infertility and suggest a possible direction for future cytotherapy to infertility.
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http://dx.doi.org/10.1155/2021/8882368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143877PMC
May 2021

Graphene-Nanorod Enhanced Quasi-Van Der Waals Epitaxy for High Indium Composition Nitride Films.

Small 2021 May 31;17(19):e2100098. Epub 2021 Mar 31.

Research and Development Center for Semiconductor Lighting Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, 100083, China.

The nitride films with high indium (In) composition play a crucial role in the fabrication of In-rich InGaN-based optoelectronic devices. However, a major limitation is In incorporation requiring a low temperature during growth at the expense of nitride dissociation. Here, to overcome this limitation, a strain-modulated growth method, namely the graphene (Gr)-nanorod (NR) enhanced quasi-van der Waals epitaxy, is proposed to increase the In composition in InGaN alloy. The lattice transparency of Gr enables constraint of in-plane orientation of nitride film and epitaxial relationships at the heterointerface. The Gr interlayer together with NRs buffer layer substantially reduces the stress of the GaN film by 74.4%, from 0.9 to 0.23 GPa, and thus increases the In incorporation by 30.7%. The first principles calculations confirm that the release of strain accounts for the dramatic improvement. The photoluminescence peak of multiple quantum wells shifts from 461 to 497 nm and the functionally small-sized cyan light-emitting diodes of 7 × 9 mil are demonstrated. These findings provide an efficient approach for the growth of In-rich InGaN film and extend the applications of nitrides in advanced optoelectronic, photovoltaic, and thermoelectric devices.
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http://dx.doi.org/10.1002/smll.202100098DOI Listing
May 2021

Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.

Drug Des Devel Ther 2021 16;15:589-600. Epub 2021 Feb 16.

Department of Nephrology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong Province, 250012, People's Republic of China.

Background: Diabetic Nephropathy remains a major cause of morbidity and mortality in patients suffering from renal dysfunction. This study accessed the nephroprotective role of Adropinin against streptozotocin (STZ) induced diabetic nephropathy in rats and scrutinizes the possible mechanism of action.

Methods: STZ (45 mg/kg) dose was used for inducing diabetic nephropathy (DN) and rats were divided into different groups and received the dose-dependent treatment of Adropinin. Blood glucose level, body weight, tissue weight, antioxidant, renal, hepatic parameters, and cytokines were determined. At the end of the experimental study, renal histopathology was performed.

Results: Adropinin significantly (P<0.001) boosted plasma insulin levels and reduced the blood glucose level. Adropinin considerably increased body weight and reduced kidney weight and kidney hypertrophy. Adropinin significantly (P<0.001) reduced urine outflow, microalbumin, total protein, blood urea nitrogen (BUN), uric acid and increased the creatinine, creatinine clearance. Adropinin significantly (P<0.001) reduced the indole sulfate level in the serum, kidney and reduced in the urine. Adropinin significantly (P<0.001) reduced the total cholesterol, triglyceride, low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and increased the level of high-density lipoprotein (HDL). Adropinin significantly (P<0.001) increased the level of antioxidant enzymes such as glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and reduced the level of malonaldehyde (MDA), 8-hydroxy-2' -deoxyguanosine (8-OHdG). Adropinin significantly (P<0.001) reduced the level of interleukin-1β (IL-1β), interleukin-6 (IL-6), transforming growth factor beta (TGF-β) and increased the level of interleukin-10 (IL-10), respectively. Adropinin treatment showed improvement in renal histopathology.

Conclusion: We can say that Adropinin showed the nephroprotective effect against the STZ-induced diabetic nephropathy rats via inflammatory and antioxidant pathway.
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http://dx.doi.org/10.2147/DDDT.S294009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896734PMC
February 2021

Hysteresis-free MoS metal semiconductor field-effect transistors with van der Waals Schottky junction.

Nanotechnology 2021 Jan 7;32(13):135201. Epub 2021 Jan 7.

School of Information Science and Engineering, Wuhan University of Science and Technology, Wuhan, 430081, People's Republic of China.

Hysteresis-free and steep subthreshold swing (SS) are essential for low-power reliable electronics. Herein, MoS metal semiconductor field-effect transistors are fabricated with GeSe/MoS van der Waals Schottky junction as a local gate, in which the rectification behavior of the heterojunction offers the modulation of channel carriers. The trap-free gate interface enables the hysteresis-free characteristics of the transistors, and promises an ideal SS of 64 mV/dec at room temperature. All the devices operate with a low threshold voltage less than -1 V with desirable saturation behavior. An OR logic gate is constructed with the dual-gated MoS transistors by varying the back and top gate voltage. The strategy present here is promising for the design of low-power digital electronics based on 2D materials.
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http://dx.doi.org/10.1088/1361-6528/abd2e8DOI Listing
January 2021

The value of platelet parameters and related scoring system in predicting esophageal varices and collateral veins in patients with liver cirrhosis.

J Clin Lab Anal 2021 Mar 2;35(3):e23694. Epub 2021 Jan 2.

The Third Central Hospital of Tianjin, Tianjin, China.

Objective: To explore the value of platelet parameters and related scoring system in predicting esophageal varices and collateral veins in patients with liver cirrhosis.

Method: A total of 94 patients with liver cirrhosis diagnosed in our hospital from March 2017 to July 2018 were divided into without esophageal varices group (NEV) and esophageal varices group (EV) into mild, moderate, and severe subgroups according to the results of general gastroscopy. The differences of biological indexes among different degrees of esophageal varices and collateral veins were analyzed, and the related factors of esophageal varices and collateral veins were analyzed.

Results: PLT count and PCT decreased gradually with the increase of esophageal varices in EV group. There were significant differences in PLT count and PCT, which were negatively correlated with the degree of collateral vein in esophageal collateral vein group. The maximum cross-sectional diameter and mean diameter of esophageal collateral veins in EV group were wider than those in NEV group. Further study showed that the maximum cross-sectional total diameter and mean diameter of esophageal collateral veins in severe esophageal varices group were wider than those in NEV group and mild esophageal varices group. Sequential Logistic regression analysis showed that PCT could effectively predict the existence of esophageal varices. Platelet parameters had no significant diagnostic value in predicting peri-ECV and Para-ECV. For platelet-related FI, APRI, FIB-4, King, Lok, GUCI, and FibroQ scoring systems, multivariate Logistic regression showed that FI, FIB-4, Lok and FibroQ scoring systems could effectively predict the presence of EV and Para-ECV (P<0.05), and its Lok Index is better than other rating systems, with AUROC values of 0.773 and 0.747, respectively. There is no significant predictive value for above scoring systems of peri-ECV.

Conclusions: PCT and LOK index can effectively predict the existence of esophageal varices and para-esophageal veins in patients with liver cirrhosis, and can be used as an effective filling method for common gastroscopy and endoscopic ultrasonography to detect EV and ECV in liver cirrhosis.
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http://dx.doi.org/10.1002/jcla.23694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957998PMC
March 2021

Effect of FeO nanoparticles exposure on the treatment efficiency of phenol wastewater and community shifts in SBR system.

J Hazard Mater 2021 04 11;407:124828. Epub 2020 Dec 11.

School of Life Science, Liaoning Normal University, Dalian 116081, China. Electronic address:

Increasing magnetic FeO nanoparticles (FeO NPs) application has aroused concern about its potential environmental toxicity. During acute and chronic exposure, key enzymes involved in phenol biodegradation were promoted at 0-600 mg/L FeO NPs, while were inhibited at 800 mg/L FeO NPs, correspondingly affected phenol degradation efficiency. Lactic dehydrogenase (LDH) increased when FeO NPs exceeded 600 mg/L, indicated the more severe cell rupture at high FeO NPs concentration. At the same FeO NPs concentration, the removal of EPS further inhibited key enzymes, decreased phenol degradation, and increased LDH, indicating that the existence of EPS relieved the adverse effects on microorganisms. Spectroscopic analysis showed that protein and polysaccharide associated bonds in EPS decreased at 0-600 mg/L FeO NPs, while increased when FeO NPs exceeded 600 mg/L, which was in accordance with EPS content. Biopolymer-degrading and phenol-degrading genera increased at 0-600 mg/L FeO NPs, while decreased at FeO NPs exceeded 600 mg/L, which conformed to EPS content and phenol degradation efficiency.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124828DOI Listing
April 2021

Hysteresis-Free MoSMetal Semiconductor Field-Effect Transistors with van der Waals Schottky Junction.

Nanotechnology 2020 Dec 11. Epub 2020 Dec 11.

School of Physics and Electronics, Key Laboratory for Micro/Nano Optoelectronic Devices of Ministry of Education, Hunan University, Changsha, Hunan, CHINA.

Hysteresis-free and steep subthreshold swing () are essential for low-power reliable electronics. Herein, MoSmetal semiconductor field-effect transistors (MESFETs) are fabricated with GeSe/MoSvan der Waals Schottky junction as a local gate, in which the rectification behavior of the heterojunction offers the modulation of channel carriers. The trap-free gate interface enables the hysteresis-free characteristics of the transistors, and promises an idealof 64 mV/dec at room temperature. All the devices operate with a low threshold voltage less than -1 V with desirable saturation behavior. An OR logic gate is constructed with the dual-gated MoStransistors by varying the back and top gate voltage. The strategy present here is promising for the design of low-power digital electronics based on 2D materials.
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http://dx.doi.org/10.1088/1361-6528/abd2e8DOI Listing
December 2020

Sparse Multicategory Generalized Distance Weighted Discrimination in Ultra-High Dimensions.

Entropy (Basel) 2020 Nov 5;22(11). Epub 2020 Nov 5.

Key Lab of Statistical Modeling and Data Analysis of Yunnan Province, Yunnan University, Kunming 650091, China.

Distance weighted discrimination (DWD) is an appealing classification method that is capable of overcoming data piling problems in high-dimensional settings. Especially when various sparsity structures are assumed in these settings, variable selection in multicategory classification poses great challenges. In this paper, we propose a multicategory generalized DWD (MgDWD) method that maintains intrinsic variable group structures during selection using a sparse group lasso penalty. Theoretically, we derive minimizer uniqueness for the penalized MgDWD loss function and consistency properties for the proposed classifier. We further develop an efficient algorithm based on the proximal operator to solve the optimization problem. The performance of MgDWD is evaluated using finite sample simulations and miRNA data from an HIV study.
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http://dx.doi.org/10.3390/e22111257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712546PMC
November 2020

Interface Engineering of Silver-Based Heterostructures for CO Reduction Reaction.

ACS Appl Mater Interfaces 2020 Dec 7;12(50):56642-56649. Epub 2020 Dec 7.

Institute of Functional Nano and Soft Materials (FUNSOM) & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, 199 Ren'ai Road, Suzhou, Jiangsu 215123, China.

The production of CO from the CO reduction reaction (CORR) is of great interest in the renewable energy storage and conversion, the neutral carbon emission, and carbon recycle utilization. Silver (Ag) is one of the catalytic metals that are active for electrochemical CO reduction into CO, but the catalysis requires a large overpotential to achieve higher selectivity. Constructing a metal-oxide interface could be an effective strategy to boost both activity and selectivity of the catalysis. Herein, density functional theory (DFT) calculations were first conducted to reveal the chemical insights of the catalytic performance on the interface between metal oxide and Ag(111) (MO/Ag(111)). The results show that the *COOH intermediates can be more stabilized on the surfaces of MO/Ag(111) than pure Ag(111). The hydrogen evolution reaction on MO/Ag(111) can be suppressed due to the significantly higher Gibbs free energy for hydrogen adsorption (Δ*), thereby enhancing the selectivity toward CORR. A series of MO/Ag composites with the unique interface based on the DFT results were then introduced though a two-step approach. The as-obtained MO/Ag catalysts boosted both the CO activity and selectivity at a relatively positive potential range, especially in the case of MnO/Ag. The reduction current density on the MnO/Ag catalyst can reach 4.3 mA cm at -0.7 V (vs RHE), which is 21.5 times higher than that on pure Ag, and the overpotential of CO to CO (390 mV) possesses is much lower than that on pure Ag NPs (690 mV). This study proposes an effective design strategy to construct a metal-oxide interface for CORR based on the synergistic effect between metals and MO.
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http://dx.doi.org/10.1021/acsami.0c19031DOI Listing
December 2020

Airborne bacterial community diversity, source and function along the Antarctic Coast.

Sci Total Environ 2021 Apr 2;765:142700. Epub 2020 Oct 2.

School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China; Anhui Key Laboratory of Polar Environment and Global Change & Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China; Center for Excellence in Urban Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian 361021, China. Electronic address:

Antarctica is an isolated and relatively simple ecosystem dominated by microorganisms, providing a rare opportunity to study the spread of airborne microbes and to predict future global climate change. However, little is known about on the diversity and potential sources of microorganisms in the marine atmosphere along the Antarctica coast. Here we explored the airborne bacterial community (i.e., bacteriome) diversity, sources and functional potential along the Antarctic coast based on 16S rRNA gene amplicon sequencing of 25 bioaerosol samples collected during the 33rd Xuelong Antarctic scientific expedition. The results showed that bacterial communities in the Antarctic bioaerosols i) were predominated by Proteobacteria (91.3%) including Sphingomonas, ii) showed relative low alpha-diversity but high spatiotemporal variabilities; and iii) were potentially immigrated with terrestrial, marine and Antarctic polar bacteria through long-range transport and sea-air exchange pathways. Moreover, canonical correspondence analysis of bacteriome composition showed that wind speed, temperature, and organic carbon had a significant effect on the bacterial community (P < 0.05), although bacterial richness (Richness index) and diversity (Simpson index and Shannon index) showed no statistically significant differences between rainy, cloudy and snowy weather conditions (Adjust P > 0.05, ANOVA, Tukey HSD test). iv) The functional profiles predicted by Tax4fun2 suggest high representation of function genes related to fatty acid biosynthesis and metabolism, amino acid metabolism, nucleotide metabolism, and carbohydrate metabolism, which is conducive to the formation of microlayers on the surface of the ocean and the survival and growth of bacteria.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142700DOI Listing
April 2021

A Robust and Low-Power Bismuth Doped Tin Oxide Memristor Derived from Coaxial Conductive Filaments.

Small 2020 Nov 14;16(46):e2004619. Epub 2020 Oct 14.

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.

Memristor, processing data storage and logic operation all-in-one, is an advanced configuration for next generation computer. In this work, a bismuth doped tin oxide (Bi:SnO ) memristor with ITO/Bi:SnO /TiN structure has been fabricated. Observing from transmission electron microscope (TEM) for the Bi:SnO device, it is found that the bismuth atoms surround the surface of SnO crystals to form the coaxial Bi conductive filament. The self-compliance current, switching voltage and operating current of Bi:SnO memristor are remarkably smaller than that of ITO/SnO /TiN device. With the content of 4.8% Bi doping, the SET operating power of doped device is 16 µW for ITO/Bi:SnO /TiN memory cell of 0.4 × 0.4 µm , which is cut down by two orders of magnitude. Hence, the findings in this study suggest that Bi:SnO memristors hold significant potential for application in low power memory and broadening the understanding of existing resistive switching (RS) mechanism.
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http://dx.doi.org/10.1002/smll.202004619DOI Listing
November 2020

Evaluation of HBV serological markers in treatment-naïve HBV mono-infected patients and HBV-HIV co-infected patients.

Virus Res 2020 12 13;290:198117. Epub 2020 Aug 13.

Tianjin Second People's Hospital, Tianjin, 300192, PR China; Tianjin Institute of Hepatology, Tianjin, 300192, PR China. Electronic address:

Objectives: Many studies have investigated the utility of hepatitis B virus (HBV) serological markers in HBV-infected patients. However, only a few studies have examined HBV serological markers in HBV-human immunodeficiency virus (HIV) co-infected patients. Here, we conducted a cross-sectional study to evaluate correlations of HBV serological markers in treatment-naïve HBV mono-infected patients and HBV-HIV co-infected patients.

Methods: HBsAg, HBV DNA, HBV RNA, and HBcrAg were quantified in 51 HBV mono-infected patients and 33 HBV-HIV co-infected patients recruited at Tianjin Second People's Hospital from 2016 to 2019.

Results: There was no significant difference in serum levels of HBV DNA (P = 0.056), HBV RNA (P = 0.387), HBcrAg (P = 0.714) and HBsAg (P = 0.165) between the patient groups. In HBV mono-infected patients, strong positive correlations were confirmed between HBV RNA and HBV DNA (r=0.620, P < 0.01), HBcrAg and HBV DNA (r=0.802, P < 0.001), and HBcrAg and HBV RNA (r=0.727, P < 0.01). In HBV-HIV co-infected patients, serum HBsAg was very strongly correlated with HBcrAg (r=0.838, P < 0.001). In HBeAg-positive HBV mono-infected patients, all HBV serological markers correlated with each other, whereas only HBV RNA correlated with HBcrAg in HBeAg-negative HBV mono-infected patients (r=0.688, P = 0.007). In HBeAg-positive HBV-HIV co-infected patients, only HBsAg correlated with HBcrAg (r=0.725, P<0.001), whereas HBcrAg and HBV RNA correlated with each other in HBeAg-negative patients (r = 0.683, P=0.010). Moreover, CD4 T-cell counts were not significantly associated with HBsAg, HBV DNA, HBV RNA, and HBcrAg levels.

Conclusion: Compared with HBsAg and HBV DNA, which are widely used in clinical settings, our study confirmed that new HBV serological markers, such as HBV RNA and HBcrAg, have some utility in HBV mono-infected patients and HBV-HIV co-infected patients for monitoring the progression of liver disease.
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http://dx.doi.org/10.1016/j.virusres.2020.198117DOI Listing
December 2020

Screening for Cognitive Impairment, Being Cognizant of the Liminal Deities and Demons.

Can J Neurol Sci 2020 11 10;47(6):731-733. Epub 2020 Jul 10.

Department of Neurosciences, Alberta Health Services, Edmonton, Canada.

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http://dx.doi.org/10.1017/cjn.2020.137DOI Listing
November 2020

A new prenylated coumestan from the roots of .

J Asian Nat Prod Res 2021 Aug 2;23(8):789-795. Epub 2020 Jul 2.

Institute of Materia Medica, Dali University, Dali 671000, China.

A new prenylated coumestan, campylohirtin A (), along with fifteen phenolic known compounds () and four other known compounds (), was obtained from the 95% ethanol extract of roots of . Their structures were elucidated based on extensive spectroscopic analysis (1 D and 2 D-NMR, MS, UV and IR). antimalarial activities of compounds -, - and were evaluated by -hematin formation inhibition assay. Compared with the positive control chloroquine diphosphate, compounds , and exhibited strong antimalarial activity with the IC values of 69.9, 33.2 and 75.4 µM, respectively. Compounds -, - and showed moderate antimalarial activities with IC values ranging from 134.6 µM to 578.6 µM.[Formula: see text].
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http://dx.doi.org/10.1080/10286020.2020.1779706DOI Listing
August 2021

Liquid profiling in plants: identification and analysis of extracellular metabolites and miRNAs in pollination drops of Ginkgo biloba.

Tree Physiol 2020 10;40(10):1420-1436

College of Horticulture and Plant Protection, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, China.

The pollination drop (PD), also known as an ovular secretion, is a critical feature of most wind-pollinated gymnosperms and function as an essential component of pollination systems. However, the metabolome and small RNAs of gymnosperm PDs are largely unknown. We employed gas chromatography-mass spectrometry to identify a total of 101 metabolites in Ginkgo biloba L. PDs. The most abundant metabolites were sugars (45.70%), followed by organic acids (15.94%) and alcohols (15.39%) involved in carbohydrate metabolism, glycine, serine and threonine metabolism. Through pollen culture of the PDs, we further demonstrated that the metabolic components of PDs are indispensable for pollen germination and growth; in particular, organic acids and fatty acids play defensive roles against microbial activity. In addition, we successfully constructed a small RNA library and detected 45 known and 550 novel miRNAs in G. biloba PDs. Interestingly, in a comparative analysis of miRNA expression between PDs and ovules, we found that most of the known miRNAs identified in PDs were also expressed in the ovules, implying that miRNAs in PDs may originate from ovules. Further, combining with potential target prediction, degradome validation and transcriptome sequencing, we identified that the interactions of several known miRNAs and their targets in PDs are involved in carbohydrate metabolism, hormone signaling and defense response pathways, consistent with the metabolomics results. Our results broaden the knowledge of metabolite profiling and potential functional roles in gymnosperm PDs and provide the first evidence of extracellular miRNA functions in ovular secretions from gymnosperms.
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http://dx.doi.org/10.1093/treephys/tpaa073DOI Listing
October 2020

Bioactive hydroxypropionylated glucose derivatives from .

Nat Prod Res 2020 Jun 11:1-9. Epub 2020 Jun 11.

Institute of Materia Medica, Dali University, Dali, P. R. China.

Four previously undescribed hydroxypropionylated d-glucose derivatives, astrabhotins A-D (-), along with ten known compounds -d-glucose (), -d-glucose (), quebrachitol (), 3-hydroxypropionic acid (), oleic acid (), isoliquiritigenin (), liquiritigenin (), odoratin (), 7-hydroxysitosterol () and daucosterol (), were isolated from the roots of . Their structures were elucidated based on the analyses of extensive spectroscopic data and physicochemical properties. Astrabhotin A () reduced the writhing response remarkably with 52.5% inhibition by acetic acid induced writhing test. The analgesic effect of was stronger than the standard drug aspirin. In addition, compounds and showed significant antioxidant activities with IC values of 9.9 ± 0.2 and 7.9 ± 0.4 μg/mL, and exhibited weak or moderate cytotoxicity against HepG2 cells with IC values of 106.6 ± 2.7 and 42.0 ± 0.9 μg/mL, respectively.
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http://dx.doi.org/10.1080/14786419.2020.1777410DOI Listing
June 2020

Overexpression of RPN2 suppresses radiosensitivity of glioma cells by activating STAT3 signal transduction.

Mol Med 2020 05 13;26(1):43. Epub 2020 May 13.

Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No.199 Dazhi Street, Nangang District, Harbin, 150001, Heilongjiang, China.

Background: Radiation therapy is the primary method of treatment for glioblastoma (GBM). Therefore, the suppression of radioresistance in GBM cells is of enormous significance. Ribophorin II (RPN2), a protein component of an N-oligosaccharyl transferase complex, has been associated with chemotherapy drug resistance in multiple cancers, including GBM. However, it remains unclear whether this also plays a role in radiation therapy resistance in GBM.

Methods: We conducted a bioinformatic analysis of RPN2 expression using the UCSC Cancer Genomics Browser and GEPIA database and performed an immunohistochemical assessment of RPN2 expression in biopsy specimens from 34 GBM patients who had received radiation-based therapy. We also studied the expression and function of RPN2 in radiation-resistant GBM cells.

Results: We found that RPN2 expression was upregulated in GBM tumors and correlated with poor survival. The expression of RPN2 was also higher in GBM patients with tumor recurrence, who were classified to be resistant to radiation therapy. In the radiation-resistant GBM cells, the expression of RPN2 was also higher than in the parental cells. Depletion of RPN2 in resistant cells can sensitize these cells to radiation-induced apoptosis, and overexpression of RPN2 had the reverse effect. Myeloid cell leukemia 1 (MCL1) was found to be the downstream target of RPN2, and contributed to radiation resistance in GBM cells. Furthermore, STAT3 was found to be the regulator of MCL1, which can be activated by RPN2 dysregulation.

Conclusion: Our study has revealed a novel function of RPN2 in radiation-resistant GBM, and has shown that MCL1 depletion or suppression could be a promising method of therapy to overcome the resistance promoted by RPN2 dysregulation.
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http://dx.doi.org/10.1186/s10020-020-00171-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222591PMC
May 2020

Mesoporous anion-cation-codoped CoS nanorings for enhanced electrocatalytic oxygen evolution reactions.

Nanotechnology 2020 Aug 6;31(33):334001. Epub 2020 May 6.

College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, Henan, People's Republic of China. College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Road, Zhengzhou 450001, People's Republic of China.

Recently, the design and synthesis of CoS micro/nanostructures have attracted attention as electrochemical energy storage and conversion devices due to their low cost and environmental friendliness. Herein, CoS nanorings were synthesized via a one-step solvothermal method with the incorporation of Fe ions, subsequently, properly selenized to boost their electrocatalytic performance. The morphology and structure of the series of cation and anion regulated CoS nanorings were characterized, the electrochemical oxygen evolution reaction (OER) properties were assessed. It is worth noting that the as-prepared catalysts, especially the innovative Fe and Se ions double doped CoS nanorings, denoted as Se/Fe-CoS-0.14, exhibited good electrocatalytic OER performance with low overpotential (298 mV) and high durability under alkaline conditions. This work provides a new perspective to develop non-noble metal CoS-based OER electrocatalysts with a superior electrocatalytic performance.
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http://dx.doi.org/10.1088/1361-6528/ab90b8DOI Listing
August 2020

Antimalarial Eudesmane Sesquiterpenoids from .

J Nat Prod 2020 04 1;83(4):927-936. Epub 2020 Apr 1.

Eleven new angeloylated eudesmane sesquiterpenoids, dobinins D-N (, , , , , , and -), and four known compounds (, , , and ) were isolated from the roots of . A new oxidation product () was also obtained from dobinin H (). Their structures were elucidated by spectroscopic data and single-crystal X-ray diffraction analyses. Dobinins K-N (-) are the first examples of rearrangement noreudesmane analogue sesquiterpenoids with a unique 6/5-fused carbon skeleton. A putative biosynthetic pathway of compounds - is proposed. Compound exhibited significant antimalarial activity superior to artemisinin with the inhibition ratio of 59.1%, and compounds , , and exhibited moderate antimalarial activities against BY265RFP with inhibition ratios ranging from 14.5% to 18.5% at a dose of 30 mg/kg/day. In addition, the apoptosis of BY265RFP by the depolarization of mitochondrial membrane potential with striking ROS production, after parasitized erythrocyte lysis mediated by cytokines IL-12 and IFN-γ, may be a possible mechanism of antimalarial action of compound against BY265RFP.
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http://dx.doi.org/10.1021/acs.jnatprod.9b00761DOI Listing
April 2020

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus.

Ann Clin Microbiol Antimicrob 2020 Mar 26;19(1):10. Epub 2020 Mar 26.

Department of Orthopedics, Southwest Hospital, Army Medical University, Gaotanyan Main Street 30#, District Shapingba, Chongqing, China.

Background: Staphylococcus aureus is a primary pathogen of orthopedic infections. By mediating antimicrobial resistance, S. aureus biofilm plays an important role in the recalcitrance of orthopedic infections, especially for the intractable osteomyelitis (OM). This study investigated the relationship between biofilm production and various genetic or phenotypic characteristics among orthopedic S. aureus strains.

Methods: A total of 137 orthopedic S. aureus isolates were enrolled and divided into OM and non-OM groups. Biofilm production was evaluated using the crystal violet assay. Genetic and phenotypic characteristics including MRSA identification, MLST and spa typing, carriage of virulence genes, drug resistance, and patients' inflammatory responses indicators were characterized. The relationship between biofilm production and above-mentioned features was respectively analyzed among all isolates and compared between OM and non-OM isolates.

Results: Biofilm production presented no significant difference between OM (including 9 MRSA isolates) and non-OM (including 21 MRSA isolates) strains. We found that ST88, t377 and ST630-MSSA-t377 strains produced very strong biofilms, while MLST types of ST15, ST25, ST398, ST5, ST59 and spa types of t002, t2325, t437 tended to produce weaker biofilms. Strains with the following profiles produced stronger biofilms: fib(+)-hlgv(+)-lukED(+)-sei(-)-sem(-)-seo(-) for all isolates, sei(-)-sem(-)-seo(-) for OM isolates, and cna (+)-fib (+)-hlgv (+)-lukED (+)-seb(-)-sed(-) for non-OM isolates. In addition, not any single drug resistance was found to be related to biofilm production. We also observed that, among OM patients, strains with stronger biofilms caused weaker inflammatory responses.

Conclusion: Some genetic or phenotypic characteristics of orthopedic strains were associated with biofilm production, and this association could be different among OM and non-OM strains. The results are of great significance for better understanding, evaluating and managing different kinds of biofilm-associated orthopedic infections, and provide potential targets for biofilm clearance.
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http://dx.doi.org/10.1186/s12941-020-00352-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099788PMC
March 2020

Verapamil extends lifespan in by inhibiting calcineurin activity and promoting autophagy.

Aging (Albany NY) 2020 03 24;12(6):5300-5317. Epub 2020 Mar 24.

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, Shanghai, China.

Previous evidence has revealed that increase in intracellular levels of calcium promotes cellular senescence. However, whether calcium channel blockers (CCBs) can slow aging and extend lifespan is still unknown. In this study, we showed that verapamil, an L-type calcium channel blocker, extended the () lifespan and delayed senescence in human lung fibroblasts. Verapamil treatment also improved healthspan in as reflected by several age-related physiological parameters, including locomotion, thrashing, age-associated vulval integrity, and osmotic stress resistance. We also found that verapamil acted on the α1 subunit of an L-type calcium channel in . Moreover, verapamil extended worm lifespan by inhibiting calcineurin activity. Furthermore, verapamil significantly promoted autophagy as reflected by the expression levels of LGG-1/LC3 and the mRNA levels of autophagy-related genes. In addition, verapamil could not further induce autophagy when , calcineurin gene, was knocked down, indicating that verapamil-induced lifespan extension is mediated via promoting autophagy processes downstream of calcineurin. In summary, our study provided mechanistic insights into the anti-aging effect of verapamil in .
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http://dx.doi.org/10.18632/aging.102951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138547PMC
March 2020

New sesquiterpenoids bearing 11-methyl ester group of agarwood.

Fitoterapia 2020 Jun 18;143:104557. Epub 2020 Mar 18.

Hainan Engineering Research Center of Agarwood, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. Electronic address:

Five new sesquiterpenoids (1-5), together with a known compound 6 was isolated from ethyl ether extract of agarwood. Their structures were elucidated on the basis of spectroscopic techniques (UV, IR, MS, 1D and 2D NMR), as well as by comparison with literature data. Compound 5 exhibited inhibitory activity against acetylcholinesterase with inhibition ratio of 48.33 ± 0.17% at the concentration of 50 μg/mL.
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http://dx.doi.org/10.1016/j.fitote.2020.104557DOI Listing
June 2020

Enhanced treatment performance of phenol wastewater and membrane antifouling by biochar-assisted EMBR.

Bioresour Technol 2020 Mar 6;306:123147. Epub 2020 Mar 6.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

Biochar-assisted EMBR (BC-assisted EMBR) was built to enhance treatment performance of phenol wastewater and membrane antifouling. BC-assisted EMBR significantly increased phenol degradation efficiency, owing to combined effects of biodegradation, adsorption and electro-catalytic degradation. Meanwhile, BC-assisted EMBR obviously mitigated membrane fouling. The coupling effect of BC and voltage led to the lower N-acyl-homoserine lactones (AHLs) and bound extracellular polymeric substances (bound EPS) contents around and on membrane surface. Protein (PN)/polysaccharide (PS) in bound EPS was decreased, led to the increase of negative charge and decrease of hydrophobicity of sludge, which abated bound EPS adsorption on membrane surface. Microbial community analyses revealed that the coupling effect of BC and voltage could enrich phenol-degraders (e.g., Comamonas), electron transfer genus (Phaselicystis), and biopolymer-degraders (Phaselicystis and Tepidisphaera) in BC-assisted EMBR and on its membrane surface, while decrease biofilm-former (e.g., Acinetobacter) and bound EPS-producer (Devosia), which was beneficial to promote phenol treatment and mitigate membrane fouling.
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http://dx.doi.org/10.1016/j.biortech.2020.123147DOI Listing
March 2020

Reliability and validity of Handwriting Test for Preschool Children (HT-PRE): A new tool to assess the handwriting ability of preschool children aged 5-6 years old in Mainland China.

PLoS One 2020 2;15(3):e0229786. Epub 2020 Mar 2.

Department of Child Health Care, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiang Su, China.

Background: Handwriting ability is related to many neuronal functions, such as visual-perceptual skills, orthographic coding, motor planning and execution, kinesthetic feedback and visual-motor coordination. To date, there is no specific assessment tool for to assess preschool children's handwriting ability in Mainland China. Our study aimed to develop a tool to assess the handwriting ability of children aged 5-6 years old in Mainland China and to analyze its reliability and validity.

Methods: The investigation comprised three phases: 1) original tool generation, 2) tool revision, 3) reliability analysis (i.e., interrater, test-retest) and validity analysis (i.e., content, criterion).

Results: The sample included a total of 482 children. The internal consistency (Cronbach alpha) was 0.74. The test-retest correlation coefficients ranged from 0.38 to 0.80. As expected, our data showed an improving trend in handwriting, and differences in respect to age and gender. When compared with the 'handwriting difficulty' group, each subtest score of children in the 'normal' group showed significant differences (p < 0.05). The correlation validity, compared with the visual-motor integration development test (VMI), was 0.17-0.52.

Conclusion: The Handwriting Test for Preschool Children (HT-PRE), which is a newly developed handwriting screening tool for preschool children aged 5-6 years old in Mainland China, has displayed a very good internal consistency, acceptable test-retest reproducibility, and good criterion-based validity, and has also shown good application prospects for handwriting difficulty screening in a clinical setting.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229786PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051084PMC
June 2020