Publications by authors named "Behnaz Jahanbin"

15 Publications

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Association between colorectal cancer and Fusobacterium nucleatum and Bacteroides fragilis bacteria in Iranian patients: a preliminary study.

Infect Agent Cancer 2021 Jun 9;16(1):41. Epub 2021 Jun 9.

Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Background And Aim: Recent studies have proposed that commensal bacteria might be involved in the development and progression of gastrointestinal disorders such as colorectal cancer (CRC). Therefore, in this study, the relative abundance of Fusobacterium nucleatum, Bacteroides fragilis, Streptococcus bovis/gallolyticus, and Enteropathogenic Escherichia coli (EPEC) in CRC tissues, and their association with clinicopathologic characteristics of CRC was investigated in Iranian patients. Moreover, the role of these bacteria in the CRC-associated mutations including PIK3CA, KRAS, and BRAF was studied.

Method: To these ends, the noted bacteria were quantified in paired tumors and normal tissue specimens of 30 CRC patients, by TaqMan quantitative Real-Time Polymerase Chain Reaction (qPCR). Next, possible correlations between clinicopathologic factors and mutations in PIK3CA, KRAS, and BRAF genes were analyzed.

Results: In studied samples, B. fragilis was the most abundant bacteria that was detected in 66 and 60% of paired tumor and normal samples, respectively. Furthermore, 15% of the B. fragilis-positive patients were infected with Enterotoxigenic B. fragilis (ETBF) in both adenocarcinoma and matched adjacent normal samples. F. nucleatum was also identified in 23% of tumors and 13% of adjacent normal tissue samples. Moreover, the relative abundance of these bacteria determined by 2 was significantly higher in CRC samples than in adjacent normal mucosa (p < 0.05). On the other hand, our findings indicated that S. gallolyticus and EPEC, compared to adjacent normal mucosa, were not prevalent in CRC tissues. Finally, our results revealed a correlation between F. nucleatum-positive patients and the KRAS mutation (p = 0.02), while analyses did not show any association between bacteria and mutation in PIK3CA and BRAF genes.

Conclusion: The present study is the first report on the analysis of different bacteria in CRC tissue samples of Iranian patients. Our findings revealed that F. nucleatum and B. fragilis might be linked to CRC. However, any link between gut microbiome dysbiosis and CRC remains unknown.
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http://dx.doi.org/10.1186/s13027-021-00381-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191199PMC
June 2021

Development of a new method for isolation of urban air particulates deposited in the human lung tissue.

Chemosphere 2021 Oct 27;280:130585. Epub 2021 Apr 27.

Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran; Department of Research Methodology and Data Analysis, Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Particulate matters (PMs) are important pollutants in urban air pollution because of their variable composition. The pulmonary clearance of PMs is critical to prevent long-term immunological responses. This study established a new method for the isolation of probably deposited urban air particulates from the human lung tissue, to investigate the features of uncleared particulates. The lung samples were acellularized with SDS solution of various concentrations ranging from 1 to 10%to lyse cells and release the PMs. In addition, the extracellular matrix (ECM) that remained was digested by proteinase K enzyme. The results of this study demonstrated that an SDS solution of 4% is the optimum concentration for the isolation of settled PMs from the lung tissue. Moreover, the used enzymatic method could separate settled PMs from the lung ECM appropriately. The results exhibited that epithelial cells form 46% of the samples' weight on average, whereas just 20% of isolated PMs were found in this part of the tissue. Both groups of separated PMs tend to agglomerate, but it is significantly higher in cellular isolated PMs. The particles separated from ECM have an agglomeration tendency, which is observable only by FE-SEM imaging. Moreover, we found a major part of urban air PMs deposited in ECM. The established method in this study can be used in future investigations to isolate other types of PMs settled in the lung, such as occupationally inhaled carbonaceous particulates.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130585DOI Listing
October 2021

Particulates induced lung inflammation and its consequences in the development of restrictive and obstructive lung diseases: a systematic review.

Environ Sci Pollut Res Int 2021 May 29;28(20):25035-25050. Epub 2021 Mar 29.

Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Particulate matters (PMs) are significant components of air pollution in the urban environment. PMs with aerodynamic diameter less than 2.5 μm (PM) can penetrate to the alveolar area and introduce numerous compounds to the pneumocystis that can initiate inflammatory response. There are several questions about this exposure as follows: does PM-induced inflammation lead to a specific disease? If yes, what is the form of the progressed disease? This systematic review was designed and conducted to respond to these questions. Four databases, including Web of Science, Scopus, PubMed, and Embase, were reviewed systematically to find the related articles. According to the included articles, the only available data on the inflammatory effects of PM comes from either in vitro or animal studies. Both types of studies have shown that the induced inflammation is type I and includes secretion of proinflammatory cytokines. The exposure duration of longer than 28 weeks was not observed in any of the reviewed studies. However, as there is not a specific antigenic component in the urban particulate matters and based on the available evidence, the antigen-presenting is not a common process in the inflammatory responses to PM. Therefore, neither signaling to repair cells such as fibroblasts nor over-secretion of extracellular matrix (ECM) proteins can occur following PM-induced inflammation. These pieces of evidence weaken the probability of the development of fibrotic diseases. On the other hand, permanent inflammation induces the destruction of ECM and alveolar walls by over-secretion of protease enzymes and therefore results in progressive obstructive effects.
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http://dx.doi.org/10.1007/s11356-021-13559-5DOI Listing
May 2021

Solitary metastases of lower extremity myxoid liposarcoma to breast: A case report and review of literature.

Clin Case Rep 2021 Mar 18;9(3):1433-1437. Epub 2021 Jan 18.

Department of Pathology Tehran University of Medical Sciences Tehran Iran.

Solitary breast metastases from myxoid liposarcoma are extremely rare. Resection with negative margins seems as an effective treatment leading to improved survival.
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http://dx.doi.org/10.1002/ccr3.3793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981608PMC
March 2021

Proven Aspergillus flavus pulmonary aspergillosis in a COVID-19 patient: A case report and review of the literature.

Mycoses 2021 Feb 11. Epub 2021 Feb 11.

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Severe COVID-19 patients complicated with aspergillosis are increasingly reported. We present a histopathological proven case of fatal COVID-19-associated pulmonary aspergillosis (CAPA), due to Aspergillus flavus. This report and existing published literature indicate diagnostic challenges and poor outcomes of CAPA in ICU patients.
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http://dx.doi.org/10.1111/myc.13255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014135PMC
February 2021

Diagnostic Value of TROP2 Expression in Papillary Thyroid Carcinoma.

Appl Immunohistochem Mol Morphol 2021 03;29(3):218-222

Department of Pathology Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Papillary thyroid carcinoma (PTC) represents the most common primary malignant thyroid lesion in all age groups. As diagnosis of PTC could be challenging in some cases and borderline nuclear features could be seen in some benign mimickers, several immunohistochemical markers are proposed to be helpful for making the correct diagnosis. In this study the authors investigated the diagnostic value of transmembrane glycoprotein (TROP2) for differentiating PTC from other thyroid follicular lesions. A total of 155 total thyroidectomy specimens including 84 cases of PTC and 71 cases of non-PTC were investigated. Among non-PTC group, 45 cases were follicular neoplasms and 26 cases were Hashimoto thyroiditis. TROP2 expression was observed in 82 of 84 cases of PTC group. In contrast only 5 cases of non-PTC group, all from Hashimoto thyroiditis specimens, showed positive expression. The sensitivity, specificity, positive predictive value, and negative predictive value of TROP2 for diagnosis of PTC was 98%, 93%, 94%, and 97%, respectively. The authors concluded that high sensitivity and specificity of TROP2 as well as its uniform negative reaction in follicular adenoma and carcinoma makes it a valuable immunohistochemical marker for diagnosis of PTC.
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http://dx.doi.org/10.1097/PAI.0000000000000886DOI Listing
March 2021

Histology of Pregnancy-Associated Breast Cancer.

Adv Exp Med Biol 2020 ;1252:81-86

Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.

Breasts are one of the most common sites of neoplastic lesions in women during pregnancy and lactation. This chapter reviews carcinomas of the breast during pregnancy and lactation while focusing on histologic features, biomarker profiles and some involved molecular pathways. Also, a brief review of previous studies on this field is performed.
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http://dx.doi.org/10.1007/978-3-030-41596-9_10DOI Listing
September 2020

Breast Cytology and Pathology in Pregnancy and Lactation.

Adv Exp Med Biol 2020 ;1252:27-32

Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical, Tehran, Iran.

Breast tissue reveals some physiologic changes during pregnancy and lactation due to hormonal alterations. Whole range of breast diseases including inflammatory, benign and malignant neoplasms can be seen in pregnancy but due to concurrent physiologic changes, may lead to diagnostic challenges. This chapter reviews sampling methods and histologic features of common benign breast lesions in pregnancy and lactation periods.
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http://dx.doi.org/10.1007/978-3-030-41596-9_4DOI Listing
September 2020

Pathological Findings of Postmortem Biopsies From Lung, Heart, and Liver of 7 Deceased COVID-19 Patients.

Int J Surg Pathol 2021 Apr 19;29(2):135-145. Epub 2020 Jun 19.

48439Tehran University of Medical Sciences, Tehran, Iran.

. A novel coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been affecting almost all nations around the world. Most infected patients who have been admitted to intensive care units show SARS signs. In this study, we aimed to achieve a better understanding of pathological alterations that take place during the novel coronavirus infection in most presumed affected organs. . We performed postmortem core needle biopsies from lung, heart, and liver on 7 deceased patients who had died of coronavirus disease 2019. Prepared tissue sections were observed by 2 expert pathologists. . Diffuse alveolar damage was the main pathologic finding in the lung tissue samples. Patients with hospitalization durations of more than 10 days showed evidence of organization. Multinucleated cells in alveolar spaces and alveolar walls, atypical enlarged cells, accumulation of macrophages in alveolar spaces, and congestion of vascular channels were the other histopathologic alteration of the lung. None of our heart biopsy samples met the criteria for myocarditis. Liver biopsies showed congestion, micro- and macro-vesicular changes, and minimal to mild portal inflammation, in the majority of cases. . Similar to the previous coronavirus infection in 2003, the main pathologic finding in the lung was diffuse alveolar damage with a pattern of organization in prolonged cases. The SARS-CoV-2 infection does not cause myocarditis, and the ischemia of myocardium is the most probable justification of the observed pathologic changes in the heart. Liver tissue sections mostly showed nonspecific findings; however, ischemia of the liver can be identified in some cases.
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http://dx.doi.org/10.1177/1066896920935195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041443PMC
April 2021

The role of transferrin receptor in the Helicobacter pylori pathogenesis; L-ferritin as a novel marker for intestinal metaplasia.

Microb Pathog 2019 Jan 31;126:157-164. Epub 2018 Oct 31.

Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address:

Helicobacter pylori growth requirements is a prerequisite to invade gastric epithelium and the process of injury to gastric cells will eventually lead to gastric cancer. The aim of this study is to investigate the effect of iron challenge on the expression of genes involved in iron homeostasis. The presence of Phosphoglucosamine mutase (glmM), cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) genes and mRNA expression of Iron Regulatory Protein 2 (IRP2), Transferrin Receptor (TFRC) and Ferritin Light Chain (FTL) genes in samples of 28 normal gastric mucosa, 33 chronic gastritis, 29 gastritis with intestinal metaplasia, 29 intestinal type adenocarcinoma patients were examined by real-time PCR. Immunohistochemistry was used to analyze cellular localization and protein levels. In the all H. pylori positive tissues, particularly in the basal regions of foveolar cells, TFRC was overexpressed (P < 0.05), and regardless of the H. pylori infection, FTL was overexpressed in all patient, exclusively in metaplastic glandular cells (P < 0.05). Furthermore, overexpression of IRP2 was associated with H. pylori positive chronic gastritis and intestinal metaplasia (P < 0.05). Our findings confirm the role of transferrin receptor in H. pylori attachment into the gastric mucosa to capture iron. Overexpression of FTL gene in metaplastic cells could be considered as a research background to investigate the role of this gene in the differentiation of gastric cells into intestinal metaplasia. In addition, this gene could be suggested as a diagnostic marker to be included among the other markers routinely performed by clinical diagnostic laboratories.
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http://dx.doi.org/10.1016/j.micpath.2018.10.039DOI Listing
January 2019

Primary immature teratoma of the uterus with peritoneal and lymph node involvement, case report.

J Obstet Gynaecol 2017 Nov 9;37(8):1096-1098. Epub 2017 Jun 9.

a Imam Khomeini Hospital Complex , Tehran University of Medical Sciences , Tehran , Iran.

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http://dx.doi.org/10.1080/01443615.2017.1312309DOI Listing
November 2017

Dicrocoelium Dentriticum in Explanted Liver: Report of an Unusual Finding.

Exp Clin Transplant 2017 Feb;15(Suppl 1):178-181

Pathology Department, Cancer Research Institute, Liver Transplant Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Dicrocoelium dentriticum, a member of trematode type helminths, is a liver parasite of ruminants. Humans are infected accidentally by ingestion of intermediate host, through infected ants via eating of raw vegetables or drinking of contaminated water. Infection is often asymptomatic or results in subtle symptoms; therefore, infections are usually unrecognized. However, it can produce chronic cholangitis and swelling or adenomatous proliferation in the bile ducts and lead to abdominal pain, diarrhea, fatigue, jaundice, and other symptoms. We report a 49-year-old female patient with end-stage hepatic cirrhosis from viral hepatitis B and D coinfection who underwent liver transplant. Shortly after transplant, she developed symptoms suggesting an obstructed biliary duct. Liver needle biopsy was done 24 hours after transplant to rule out rejection. Biopsy of her explanted liver was also examined pathologically. Microscopic examination of the liver needle biopsy ruled out rejection. Prepared sections of explanted liver revealed a helminth in the common bile duct. Morphologic reconstruction of helminth by microscopic findings and consultation with an expert parasitologist supported the diagnosis of Dicrocoelium dentriticum.
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http://dx.doi.org/10.6002/ect.mesot2016.P62DOI Listing
February 2017

Peripheral T-Cell Lymphoma Presenting as a Primary Uterine Cervix Mass: A Report of a Rare Case.

Int J Gynecol Pathol 2017 Nov;36(6):523-527

Departments of Pathology and Laboratory Medicine, Sina Hospital (F.K.) Pathology (N.N., F.N., B.J.) Radiology (M.M.), Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran.

Malignant lymphoma is a rare form of malignancy in the uterine cervix. Most of the previously reported cases were B-cell lymphomas. We present a case of primary peripheral T-cell lymphoma, not otherwise specified, in the uterine cervix in a 49-year-old woman with a history of severe vaginal bleeding. The patient was treated with a CHOP (cyclophosphamide, adriamycin, vincristine, and prednisolone) chemotherapy regimen and 28 cycles of radiotherapy. Three months later, she presented with generalized lymphadenopathy and sudden left-eye ptosis as a complication of CNS involvement and died after 20 months from the initial disease presentation.
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http://dx.doi.org/10.1097/PGP.0000000000000358DOI Listing
November 2017

Malignant ovarian germ cell tumours in gonadal Y chromosome mosaicism.

J Clin Pathol 2011 Nov 12;64(11):973-6. Epub 2011 Jul 12.

Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran.

Aims: Previous studies have shown that two partially overlapping mechanisms are responsible for the development of malignant ovarian germ cell tumours (MOGCT): either spontaneous mutations, mostly in KIT gene, or the presence of Y chromosome material, in dysgenetic gonads. While unilateral oophorectomy and preservation of fertility is favourable in most cases, presence of whole or part of Y chromosome in dysgenetic ovaries is associated with a risk of bilateral germ cell tumour development. The aim of this study was to evaluate the frequency of Y chromosome material in these tumours.

Methods: A total of 47 cases with histopathologic diagnosis of malignant germ cell tumour were selected in a period of 9 years. A relative quantitative PCR (RQ-PCR) method was designed and validated to detect testis-specific protein Y-encoded (TSPY) gene on Y chromosome. After DNA extraction, TSPY gene was sought as a surrogate of Y chromosome.

Results: Significant amounts of TSPY gene were found in seven cases, two of which had gonadoblastoma and one had cytogenetic proof of Y chromosome presence.

Conclusions: Some MOGCTs develop on the background of gonadal mosaicism and gonadal dysgenesis. Bilateral oophorectomy may be indicated in patients with these disorders because they are at risk of developing an MOGCT on the contralateral gonad. Moreover, this chromosomal abnormality is hardly found by routine methods, and the abnormality is more easily sought in MOGCT cells by means of RQ-PCR.
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http://dx.doi.org/10.1136/jcp.2011.090738DOI Listing
November 2011

Bronchodilatory effect of Carum copticum in airways of asthmatic patients.

Therapie 2007 Jan-Feb;62(1):23-9. Epub 2007 Mar 21.

Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: Several therapeutic effects including anti-asthma and dyspnea have been described for the seeds of Carum copticum. In previous studies the relaxant and anticholinergic (functional antagonism) effects, histamine H(1) inhibitory and beta(2) stimulatory effects of Carum copticum have been demonstrated on guinea pig tracheal chains. In the present study, the bronchodilatory effect of boiled extract from Carum copticum in the airways of asthmatic patients was examined.

Materials And Methods: The bronchodilatory effects of 0.125 and 0.25 ml/kg of 10 g% boiled extract in comparison with 6 mg/kg theophylline and placebo were studied by measuring pulmonary function tests (PFTs) and specific airway conductance (sGaw). Pulmonary function tests were measured before administration and repeated 30, 60, 90, 120, 150 and 180 min after administration of the oral extract and theophylline.

Results: The results showed that the boiled extract of Carum copticum caused significant increases in all PFT values, in most time intervals, (p<0.05 to p<0.001). However, the increase in most PFT values due to the both doses of boiled extract were significantly lower than those of theophylline in most time intervals (p<0.05 to p<0.001). The onset of brochodilatory effect of extract was similar to that of theophylline beginning 30 min, its maximum effect on PFTs (23 to 32% increase) was seen in 90-120 min and the effect of extract decline after 150 min following administration similar to the effect of theophylline. In addition the placebo did not cause any significant increase in PFT values.

Conclusion: In conclusion, the results of the present study showed that Carum copticum has a relatively bronchodilatory effect on asthmatic airways which was comparable with the effect of theophylline at concentrations used.
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http://dx.doi.org/10.2515/therapie:2007007DOI Listing
May 2007