Publications by authors named "Barbara Wagner"

47 Publications

The effect of biofeedback interventions on pain, overall symptoms, quality of life and physiological parameters in patients with pelvic pain : A systematic review.

Wien Klin Wochenschr 2021 Mar 22. Epub 2021 Mar 22.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.

Background: Biofeedback is recognized as an effective additive method for treating certain phenotypes of chronic pelvic pain syndrome and is a therapeutic option in other pelvic pain conditions. This review aims to evaluate evidence from the literature with a focus on the effect of biofeedback on pain reduction, overall symptom relief, physiological parameters and quality of life.

Methods: A systematic literature search was conducted using the databases PubMed, MEDLINE, Embase, Cochrane Library and PEDro from inception to July 2020. Data were tabulated and a narrative synthesis was carried out, since data heterogeneity did not allow a meta-analysis. The PEDro scale and the McMaster Critical Review Form-Quantitative Studies were applied to assess risk of bias.

Results: Out of 651 studies, 37 quantitative studies of primary research evaluating pelvic pain conditions in male and female adults and children were included. They covered biofeedback interventions on anorectal disorders, chronic prostatitis, female chronic pelvic pain conditions, urologic phenotypes in children and adults and a single study on low back pain. For anorectal disorders, several landmark studies demonstrate the efficacy of biofeedback. For other subtypes of chronic pelvic pain conditions there is tentative evidence that biofeedback-assisted training has a positive effect on pain reduction, overall symptoms relief and quality of life. Certain factors have been identified that might be relevant in improving treatment success.

Conclusions: For certain indications, biofeedback has been confirmed to be an effective treatment. For other phenotypes, promising findings should be further investigated in robust and well-designed randomized controlled trials.
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http://dx.doi.org/10.1007/s00508-021-01827-wDOI Listing
March 2021

Pain management in hemophilia: expert recommendations.

Wien Klin Wochenschr 2021 Mar 4. Epub 2021 Mar 4.

Department of Pediatrics, Medical University of Innsbruck, Innrain 52, 6020, Innsbruck, Austria.

Introduction: As a typical consequence of bleeding into muscles and joints, patients with severe hemophilia suffer from acute and chronic pain. In spite of its high prevalence, pain in this patient group is not always sufficiently considered or treated in an effective manner.

Aim: The recommendations presented in this paper address possible improvements in pain management in hemophilia patients and particularities that have to be taken into account in this patient group.

Method: The manifold aspects of pain management in hemophilia patients were discussed within the framework of an expert meeting. Based on the available literature and the experts' clinical experience, the participants developed a set of recommendations presented in this paper.

Results: Pain management in patients with hemophilia is often insufficient, a fact that not only influences the patients' quality of life but also implies the risk of difficult to manage chronic pain. Both the prevalent polypharmacy (due to comorbidities) as well as the underlying disease itself present special challenges to pain therapy in this patient group. The present review and recommendations are intended to support medical professionals in recognising the risks of pain chronicity, applying basic principles of multimodal pain therapy, including the options of psychological intervention and modalities of physical medicine in therapy concepts, and reaching a comprehensive understanding of the range of analgesic options available.
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http://dx.doi.org/10.1007/s00508-020-01798-4DOI Listing
March 2021

Tracking of Glycans Structure and Metallomics Profiles in Mutated Melanoma Cells Treated with Vemurafenib.

Int J Mol Sci 2021 Jan 4;22(1). Epub 2021 Jan 4.

Faculty of Chemistry, University of Warsaw, Pasteura Str. 1, PL-02-093 Warsaw, Poland.

Nearly half of patients with advanced and metastatic melanomas harbor a mutation. Vemurafenib (VEM), a BRAF inhibitor, is used to treat such patients, however, responses to VEM are very short-lived due to intrinsic, adaptive and/or acquired resistance. In this context, we present the action of the B-Raf serine-threonine protein kinase inhibitor (vemurafenib) on the glycans structure and metallomics profiles in melanoma cells without (MeWo) and with (G-361) mutations. The studies were performed using 1-acid glycoprotein (AGP), a well-known acute-phase protein, and concanavalin A (Con A), which served as the model receptor. The detection of changes in the structure of glycans can be successfully carried out based on the frequency shifts and the charge transfer resistance after interaction of AGP with Con A in different VEM treatments using QCM-D and EIS measurements. These changes were also proved based on the cell ultrastructure examined by TEM and SEM. The LA-ICP-MS studies provided details on the metallomics profile in melanoma cells treated with and without VEM. The studies evidence that vemurafenib modifies the glycans structures and metallomics profile in melanoma cells harboring mutation that can be further implied in the resistance phenomenon. Therefore, our data opens a new avenue for further studies in the short-term addressing novel targets that hopefully can be used to improve the therapeutic regiment in advanced melanoma patients. The innovating potential of this study is fully credible and has a real impact on the global patient society suffering from advanced and metastatic melanomas.
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http://dx.doi.org/10.3390/ijms22010439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794875PMC
January 2021

Title Changes in the Mineral Composition of Rat Femoral Bones Induced by Implantation of LNCaP Prostate Cancer Cells and Dietary Supplementation.

Nutrients 2020 Dec 30;13(1). Epub 2020 Dec 30.

Faculty of Pharmacy with the Laboratory Medicine Division, Department of Bromatology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.

Prostate cancer (PCa) is the second most frequent cancer in men and the fifth most common cause of death worldwide, with an estimated 378,553 deaths in 2020. Prostate cancer shows a strong tendency to form metastatic foci in the bones. A number of interactions between cancer cells attacking bones and cells of the bone matrix lead to destruction of the bone and growth of the tumour. The last few decades have seen increased interest in the precise role of minerals in human health and disease. Tumour cells accumulate various minerals that promote their intensive growth. Bone, as a storehouse of elements, can be a valuable source of them for the growing tumour. There are also reports suggesting that the presence of some tumours, e.g., of the breast, can adversely affect bone structure even in the absence of metastasis to this organ. This paper presents the effect of chronic dietary intake of calcium, iron and zinc, administered in doses corresponding maximally to twice their level in a standard diet, on homeostasis of selected elements (Ca, K, Zn, Fe, Cu, Sr, Ni, Co, Mn and Mo) in the femoral bones of healthy rats and rats with implanted cancer cells of the LNCaP line. The experiment was conducted over 90 days. After the adaptation period, the animals were randomly divided into four dietary groups: standard diet and supplementation with Zn, Fe and Ca. Every dietary group was divided into experimental group (with implanted cancer cells) and control group (without implanted cancer cells). The cancer cells (LnCaP) were implanted intraperitoneally in the amount 1 × 10 to the rats at day 90 of their lifetime. Bone tissue was dried and treated with microwave-assisted mineral digestation. Total elemental content was quantified by ICP-MS. Student's -test and Anova or Kruskal-Wallis tests were applied in order to compare treatment and dietary groups. In the case of most of the diets, especially the standard diet, the femoral bones of rats with implanted LNCaP cells showed a clear downward trend in the content of the elements tested, which may be indicative of slow osteolysis taking place in the bone tissue. In the group of rats receiving the standard diet, there were significant reductions in the content of Mo (by 83%), Ca (25%), Co (22%), Mn (13%), K (13%) and Sr (9%) in the bone tissue of rats with implanted LNCaP cells in comparison with the control group receiving the same diet but without LNCaP implantation. Supplementation of the rat diet with calcium, zinc and iron decreased the frequency of these changes relative to the standard diet, which may indicate that the diet had an inhibitory effect on bone resorption in conditions of LNCaP implantation. The principal component analysis (PCA) score plot confirms the pronounced effect of implanted LNCaP cells and the standard diet on bone composition. At the same time, supplementation with calcium, zinc and iron seems to improve bone composition. The microelements that most often underwent quantitative changes in the experimental conditions were cobalt, manganese and molybdenum.
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http://dx.doi.org/10.3390/nu13010100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823861PMC
December 2020

Influence of Inorganic Bases on the Structure of Titanium Dioxide-Based Microsheets.

ACS Omega 2020 Sep 10;5(37):23703-23717. Epub 2020 Sep 10.

Institute of Inorganic Chemistry of the Czech Academy of Sciences, 250 68 Husinec-Řež, Czech Republic.

Laboratory synthesis of microsheets of titanium dioxide from titanyl sulfate involves the use of ammonia solution, whereas another inorganic base is most likely to be employed at the industrial level, as ammonia is a toxic agent and therefore should be avoided according to European Union (EU) regulations. Selected nontoxic bases such as sodium, potassium, and lithium hydroxides have been tested as an alternative to ammonia solution to obtain amorphous and crystalline TiO-based microsheets. The final products obtained at each step of the procedure (samples lyophilized and annealed at 230 and 800 °C) were analyzed with electron and atomic force microscopy, X-ray powder diffraction, thermal analysis, and Fourier transform infrared (FTIR) and Raman spectroscopies to determine their morphology and phase composition. The differences in the morphology of the obtained products were described in detail as well as phase and structural composition throughout the process. It was found that, in the last step of the synthesis, microsheets annealed at 800 °C were built of small rods and oval or platy crystalline particles depending on the base used. The temperature of formation of anatase, rutile, and alkali-metal titanates in correlation with the ionic radius of the alkali metal present in the sample was discussed.
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http://dx.doi.org/10.1021/acsomega.0c02570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513341PMC
September 2020

Tris(ferrocenylmethidene)sumanene: synthesis, photophysical properties and applications for efficient caesium cation recognition in water.

Dalton Trans 2020 Aug 29;49(29):9965-9971. Epub 2020 Jun 29.

Faculty of Chemistry, Warsaw University of Technology, Noakowskiego Str. 3, 00-664 Warsaw, Poland.

The synthesis of a sumanene derivative bearing three ferrocenyl substituents is presented. This conjugated compound is solution-processable, shows red-light emission with high fluorescence quantum yield and can be used for the construction of the first buckybowl-based sensor for the selective and effective recognition of caesium cations (Cs) in aqueous solution.
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http://dx.doi.org/10.1039/d0dt01506gDOI Listing
August 2020

Can reminders improve adherence to regular physical activity and exercise recommendations in people over 60 years old? : A randomized controlled study.

Wien Klin Wochenschr 2021 Jun 26;133(11-12):620-624. Epub 2020 Jun 26.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.

Purpose: The purpose of the study was to investigate whether additional reminders could enhance adherence to a 12-week program consisting of regular physical activity.

Methods: The study collective consisted of pensioners insured with the Austrian Insurance Fund for Civil or Public Servants. They were made aware of our program through the public service union. The subjects were randomized to an intervention group (group A) that received reminders and to a control group (group B) that did not receive such notifications. Adherence to physical activity was assessed by the use of diaries.

Results: Group A performed 96 min more moderate intensity regular physical activity per week than group B (group A median 269 min, r = 0-1560 min; group B median 173 min, r = 0-2700 min). The Mann-Whitney U-test showed no significant differences (p = 0.080) between the study groups. There was no difference in muscle strengthening activity (group A: median: 2, r = 0-13 sessions; group B: median: 2, r = 0-20 sessions).

Conclusion: The major positive observation was that both the experimental and control group participants exceeded the recommended level of physical activity. Nevertheless, there were some differences concerning the minutes of physical activity performed in favor of the intervention group.
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http://dx.doi.org/10.1007/s00508-020-01699-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318722PMC
June 2021

First application of focused low-energy extracorporeal shockwave therapy in a patient with severe hemophilia A and plantar fasciitis.

Wien Klin Wochenschr 2021 03 12;133(5-6):245-246. Epub 2020 Jun 12.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.

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http://dx.doi.org/10.1007/s00508-020-01687-wDOI Listing
March 2021

Phase separation in swelling and deswelling hydrogels with a free boundary.

Phys Rev E 2020 Mar;101(3-1):032501

Weierstrass Institute, Mohrenstrasse 39, 10117 Berlin, Germany.

We present a full kinetic model of a hydrogel that undergoes phase separation during swelling and deswelling. The model accounts for the interfacial energy of coexisting phases, finite strain of the polymer network, and solvent transport across free boundaries. For the geometry of an initially dry layer bonded to a rigid substrate, the model predicts that forcing solvent into the gel at a fixed rate can induce a volume phase transition, which gives rise to coexisting phases with different degrees of swelling, in systems where this cannot occur in the free-swelling case. While a nonzero shear modulus assists in the propagation of the transition front separating these phases in the driven-swelling case, increasing it beyond a critical threshold suppresses its formation. Quenching a swollen hydrogel induces spinodal decomposition, which produces several highly localized, highly swollen phases which coarsen and are then ejected from free boundary. The wealth of dynamic scenarios of this system is discussed using phase-plane analysis and numerical solutions in a one-dimensional setting.
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http://dx.doi.org/10.1103/PhysRevE.101.032501DOI Listing
March 2020

The effect of resistance exercise on strength and safety outcome for people with haemophilia: A systematic review.

Haemophilia 2020 Mar 24;26(2):200-215. Epub 2020 Feb 24.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Vienna, Austria.

Introduction: Haemophilia is a congenital bleeding disorder with severe musculoskeletal complications. Resistance exercise is important to increase joint stability and to improve physical performance.

Aim: This review aimed to investigate the safety and efficacy of resistance exercise interventions on people with haemophilia (PwH) and evaluate whether the American College of Sports Medicine resistance exercise criteria for healthy adults are valid for this population.

Methods: A systematic search in literature was conducted, using the databases PubMed, MEDLiNE, CINAHL, SCOPUS, PEDro and Cochrane Library. Out of 2.440 studies published between 1960 and November 2019, 14 studies (9 randomized controlled trials, 1 controlled trial, 4 single-group prospective studies) applying resistance exercise in juvenile and adult PwH corresponded to the inclusion criteria.

Results: Studies performed dynamic, isokinetic or a combination of isometric and dynamic resistance training. Most interventions were carried out in the context of a multimodal training. Resistance was provided using fixed and free weights, body weight, resistance bands and water resistance. Study protocols included clinical and home-based settings. Several studies suggest that training intensities lower than those known to increase the strength of healthy people are effective in increasing the strength of PwH. Resistance exercise seems to be a safe intervention if it is adequately monitored, individually adapted and applied with sufficient factor therapy. Due to the heterogeneity of study designs, training interventions and outcome measures a meta-analysis could not be performed.

Conclusions: Further studies of higher methodological quality are needed to determine the optimal types of exercise, optimal dosage and timing.
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http://dx.doi.org/10.1111/hae.13938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155123PMC
March 2020

Establishing an online physical exercise program for people with hemophilia.

Wien Klin Wochenschr 2019 Nov 18;131(21-22):558-566. Epub 2019 Sep 18.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.

Background: Hemophilia is a congenital bleeding disorder with an estimated frequency of 1:10,000 births. Repeated joint bleeding is a hallmark of the disorder and leads to painful hemophilic arthropathy. Regular exercise can help improve joint stability and function, reduce the risk of injury and bleeding and improve physical fitness and quality of life. This method paper describes an online training concept aiming to offer access to appropriate exercise instructions for people with hemophilia who are not able to attend regular training at a hemophilia center.

Methods: The online exercise program is accessible through the homepage of the Department of Physical Medicine, Rehabilitation and Occupational Medicine of the Medical University Vienna as well as through scanning a QR code printed on information material using a smart phone or tablet.

Results: The program contains exercises to improve mobility, coordination, muscular strength and flexibility. A brief introduction is given by a hematologist, a pediatrician and a physiatrist. An introductory video informs about contraindications and essential precautions, such as medical attendance and sufficient factor therapy to consider before starting the training. Another video gives advice on the exercise composition. The demonstrated exercises are explained by a physician and are available for adults and children. To individualize training recommendations and offer further diagnostic tools and physical treatment options as necessary, the Department of Physical Medicine, Rehabilitation and Occupational Medicine of the Medical University of Vienna will establish consultation hours for people with hemophilia.

Conclusion: As hemophilia is an orphan disease, patients are mainly treated in specialized centers. For patients who live far from these centers or have limited access to a training there for other reasons, the physical medicine consultation hour and the implementation of online exercise instructions offer individually adapted exercise information for a regular home-based training to benefit from increased physical fitness and joint stability.
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http://dx.doi.org/10.1007/s00508-019-01548-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851214PMC
November 2019

Typical aspects in the rehabilitation of cancer patients suffering from metastatic bone disease or multiple myeloma.

Wien Klin Wochenschr 2019 Nov 2;131(21-22):567-575. Epub 2019 Jul 2.

Department of Physical Medicine, Rehabilitation and Occupational Medicine, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.

Background: The aim of this study was to present a practical concept focusing on typical aspects of regular physical activity, exercise and physical modalities for patients suffering from metastatic bone disease or multiple myeloma.

Methods: A narrative review of the relevant scientific literature and presentation of clinical experiences.

Results: In cancer patients with metastatic bone disease or multiple myeloma, pain is treated in an interdisciplinary and multimodal setting by using medication, radiotherapy and physical medical modalities (e.g. transcutaneous electrical nerve stimulation); however, modalities increasing local blood flow, such as ultrasound therapy, thermotherapy, massage, various electrotherapy options, are not performed at the site of the tumor. For physical activity and exercise, a suitable indication of the static and dynamic capacity of the affected skeletal structures is essential. This process includes strategies to maintain and improve mobility and independence. Individually tailored and adapted physical activity and exercise concepts (programs) within a multidisciplinary and interdisciplinary setting (tumor board) are used to manage the condition and bone load-bearing capacity of the patient. Typical clinical features and complications, such as pathological fractures in patients suffering from metastatic bone disease and additionally hypercalcemia, monoclonal gammopathy with bone marrow aplasia and risk of renal failure in patients with multiple myeloma have to be considered when planning supportive strategies and rehabilitation.

Conclusion: In order to ensure the safety and effectiveness of regular physical activity, exercise, and physical modalities in patients with metastatic bone disease or multiple myeloma, typical contraindications and considerations should be noted.
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http://dx.doi.org/10.1007/s00508-019-1524-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851044PMC
November 2019

Workplace injury rates and firm-level turnover in Montana's oil and gas industry.

Am J Ind Med 2019 06 2;62(6):535-541. Epub 2019 May 2.

Workforce Services Division, Montana Department of Labor & Industry, Helena, MT.

Background: Do workers follow their self-interest by minimizing injury risk in their employment decision? If so, employers could use injury reduction as a recruitment and retention strategy. This study explores whether injury incidence is associated with turnover in Montana's Oil and Gas industry.

Methods: A panel data set of Unemployment Insurance and Workers' Compensation administrative records from 2010 to 2015 was used to model the relationship between turnover and injury claim rates at the firm level.

Results: Total turnover and injury rates were found to be positively related while injury rates and separation rates had no such association. Quarters in which the employer experienced a severe injury had a 3.3 percentage point increase in separation rates.

Discussion: The findings suggest that injured workers contribute to increased turnover, but coworker turnover does not increase with increased injury rates in the firm. Secondary findings suggest a relationship between recent hires and increased injury rates, although further investigation is required.
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http://dx.doi.org/10.1002/ajim.22983DOI Listing
June 2019

Signatures of slip in dewetting polymer films.

Proc Natl Acad Sci U S A 2019 05 19;116(19):9275-9284. Epub 2019 Apr 19.

Weierstrass Institute for Applied Analysis and Stochastics, 10117 Berlin, Germany.

Thin polymer films on hydrophobic substrates are susceptible to rupture and hole formation. This, in turn, initiates a complex dewetting process, which ultimately leads to characteristic droplet patterns. Experimental and theoretical studies suggest that the type of droplet pattern depends on the specific interfacial condition between the polymer and the substrate. Predicting the morphological evolution over long timescales and on the different length scales involved is a major computational challenge. In this study, a highly adaptive numerical scheme is presented, which allows for following the dewetting process deep into the nonlinear regime of the model equations and captures the complex dynamics, including the shedding of droplets. In addition, our numerical results predict the previously unknown shedding of satellite droplets during the destabilization of liquid ridges that form during the late stages of the dewetting process. While the formation of satellite droplets is well known in the context of elongating fluid filaments and jets, we show here that, for dewetting liquid ridges, this property can be dramatically altered by the interfacial condition between polymer and substrate, namely slip. This work shows how dissipative processes can be used to systematically tune the formation of patterns.
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http://dx.doi.org/10.1073/pnas.1820487116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510987PMC
May 2019

Impact of energy dissipation on interface shapes and on rates for dewetting from liquid substrates.

Sci Rep 2018 Sep 5;8(1):13295. Epub 2018 Sep 5.

Weierstrass Institute for Applied Analysis and Stochastics, 10117, Berlin, Germany.

We revisit the fundamental problem of liquid-liquid dewetting and perform a detailed comparison of theoretical predictions based on thin-film models with experimental measurements obtained by atomic force microscopy. Specifically, we consider the dewetting of a liquid polystyrene layer from a liquid polymethyl methacrylate layer, where the thicknesses and the viscosities of both layers are similar. Using experimentally determined system parameters like viscosity and surface tension, an excellent agreement of experimentally and theoretically obtained rim profile shapes are obtained including the liquid-liquid interface and even dewetting rates. Our new energetic approach additionally allows to assess the physical importance of different contributions to the energy-dissipation mechanism, for which we analyze the local flow fields and the local dissipation rates. Using this approach, we explain why dewetting rates for liquid-liquid systems follow no universal power law, despite the fact that experimental velocities are almost constant. This is in contrast to dewetting scenarios on solid substrates and in contrast to previous results for liquid-liquid substrates using heuristic approaches.
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http://dx.doi.org/10.1038/s41598-018-31418-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125481PMC
September 2018

Volumetry based biomarker speed of growth: Quantifying the change of total tumor volume in whole-body magnetic resonance imaging over time improves risk stratification of smoldering multiple myeloma patients.

Oncotarget 2018 May 18;9(38):25254-25264. Epub 2018 May 18.

Institute of Diagnostic and Interventional Radiology, University Medical Center Rostock, Rostock, Germany.

The purpose of this study was to improve risk stratification of smoldering multiple myeloma patients, introducing new 3D-volumetry based imaging biomarkers derived from whole-body MRI. Two-hundred twenty whole-body MRIs from 63 patients with smoldering multiple myeloma were retrospectively analyzed and all focal lesions >5mm were manually segmented for volume quantification. The imaging biomarkers total tumor volume, speed of growth (development of the total tumor volume over time), number of focal lesions, development of the number of focal lesions over time and the recent imaging biomarker '>1 focal lesion' of the International Myeloma Working Group were compared, taking 2-year progression rate, sensitivity and false positive rate into account. Speed of growth, using a cutoff of 114mm/month, was able to isolate a high-risk group with a 2-year progression rate of 82.5%. Additionally, it showed by far the highest sensitivity in this study and in comparison to other biomarkers in the literature, detecting 63.2% of patients who progress within 2 years. Furthermore, its false positive rate (8.7%) was much lower compared to the recent imaging biomarker '>1 focal lesion' of the International Myeloma Working Group. Therefore, speed of growth is the preferable imaging biomarker for risk stratification of smoldering multiple myeloma patients.
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http://dx.doi.org/10.18632/oncotarget.25402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5982766PMC
May 2018

The importance of antibody orientation in the electrochemical detection of ferritin.

Biosens Bioelectron 2018 Jun 5;109:83-89. Epub 2018 Mar 5.

Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, PL-02-093 Warsaw, Poland. Electronic address:

The way of immobilization of the monoclonal antibody (type IgG) on the electrode surface has a significant effect on the amount of the immobilized protein and in consequence on current signal of protein. Herein, we demonstrate that the application of appropriately functionalized phenyl film allowed us to control the orientation of the antibody (Ab) molecules on the electrode surface. The influence of Ab orientation on the efficiency of antigen-antibody interaction was tested with an example blood plasma protein (ferritin; Ft). To control the orientation of Ab molecules the phenyl films containing -COOH or -NH groups were applied. Contrary to aminoethylophenyl layer, the carboxyphenyl film guaranteed the shortest distance between the redox center of the protein and the electrode surface. Additionally, the application of an external magnetic field together with magnetic nanoparticles allowed achieving the best orientation to observe well-defined ferritin current signals. The proposed method of ferritin detection can be successfully used in the concentration range of Ft between 0.1 and 30 µg dL. The detection limit for a carboxyphenyl film was estimated as 0.40 ± 0.04 and 0.13 ± 0.04 µg dL for impedance and voltammetric measurements, respectively. In turn, for an aminoethylophenyl film the detection limit was 0.03 ± 0.002 (electrochemical impedance spectroscopy; EIS) and 0.02 ± 0.002 µg dL (differential pulse voltammetry, DPV). The interday precision (reproducibility) was calculated (4.10 ÷ 9.10% RSD) together with the intraday precision / repeatability (3.20 ÷ 8.0% RSD) for the studied samples. The functionality of the sensor has been tested on rat blood samples. Based on the performed investigations it can be stated that the developed sensor was characterized by high selectivity and good sensitivity.
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http://dx.doi.org/10.1016/j.bios.2018.02.063DOI Listing
June 2018

Localized instabilities and spinodal decomposition in driven systems in the presence of elasticity.

Phys Rev E 2018 Jan;97(1-1):012801

Weierstrass Institute, Mohrenstrasse 39, 10117 Berlin, Germany.

We study numerically and analytically the instabilities associated with phase separation in a solid layer on which an external material flux is imposed. The first instability is localized within a boundary layer at the exposed free surface by a process akin to spinodal decomposition. In the limiting static case, when there is no material flux, the coherent spinodal decomposition is recovered. In the present problem, stability analysis of the time-dependent and nonuniform base states as well as numerical simulations of the full governing equations are used to establish the dependence of the wavelength and onset of the instability on parameter settings and its transient nature as the patterns eventually coarsen into a flat moving front. The second instability is related to the Mullins-Sekerka instability in the presence of elasticity and arises at the moving front between the two phases when the flux is reversed. Stability analyses of the full model and the corresponding sharp-interface model are carried out and compared. Our results demonstrate how interface and bulk instabilities can be analyzed within the same framework which allows us to identify and distinguish each of them clearly. The relevance for a detailed understanding of both instabilities and their interconnections in a realistic setting is demonstrated for a system of equations modeling the lithiation and delithiation processes within the context of lithium ion batteries.
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http://dx.doi.org/10.1103/PhysRevE.97.012801DOI Listing
January 2018

Elemental imaging of heterogeneous inorganic archaeological samples by means of simultaneous laser induced breakdown spectroscopy and laser ablation inductively coupled plasma mass spectrometry measurements.

Talanta 2018 Mar 14;179:784-791. Epub 2017 Dec 14.

Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA; Applied Spectra, Inc., Fremont, CA, USA.

Multilayered fragments of murals were used to evaluate the usefulness of two laser-based instrumental methods: laser induced breakdown spectroscopy (LIBS) and laser ablation (LA) inductively coupled plasma mass spectrometry (ICP-MS) for elemental imaging of unique historic samples. Simultaneous LA/LIBS measurements with the use of 266nm Nd:YAG laser were performed on cross-sections of mediaeval Nubian objects with specific blue painting layers including either Egyptian blue (CaCuSiO) or lapis lazuli (NaAlSiOS). A combined use of both laser-based methods allowed for clear distinguishing of blue pigments based on visual imaging of a chemical composition of heterogeneous archaeological inorganic samples. The identification of the pigments was confirmed with Raman spectroscopy.
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http://dx.doi.org/10.1016/j.talanta.2017.12.011DOI Listing
March 2018

Thin-film electrodes for high-capacity lithium-ion batteries: influence of phase transformations on stress.

Proc Math Phys Eng Sci 2016 Sep;472(2193):20160093

Weierstrass Institute, Mohrenstraße 39, 10117 Berlin, Germany; Institute of Mathematics, Technische Universität Berlin, Straße des 17. Juni 136, 10623 Berlin, Germany.

In this study, we revisit experiments by Sethuraman (2010 , , 5062-5066. (doi:10.1016/j.jpowsour.2010.02.013)) on the stress evolution during the lithiation/delithiation cycle of a thin film of amorphous silicon. Based on recent work that show a two-phase process of lithiation of amorphous silicon, we formulate a phase-field model coupled to elasticity in the framework of Larché-Cahn. Using an adaptive nonlinear multigrid algorithm for the finite-volume discretization of this model, our two-dimensional numerical simulations show the formation of a sharp phase boundary between the lithiated and the amorphous silicon that continues to move as a front through the thin layer. We show that our model captures the non-monotone stress loading curve and rate dependence, as observed in recent experiments and connects characteristic features of the curve with the structure formation within the layer. We take advantage of the thin film geometry and study the corresponding one-dimensional model to establish the dependence on the material parameters and obtain a comprehensive picture of the behaviour of the system.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5046981PMC
http://dx.doi.org/10.1098/rspa.2016.0093DOI Listing
September 2016

Quantitative aspects of inductively coupled plasma mass spectrometry.

Philos Trans A Math Phys Eng Sci 2016 Oct;374(2079)

Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury str. 101, Warszawa 02-089, Poland.

Accurate determination of elements in various kinds of samples is essential for many areas, including environmental science, medicine, as well as industry. Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful tool enabling multi-elemental analysis of numerous matrices with high sensitivity and good precision. Various calibration approaches can be used to perform accurate quantitative measurements by ICP-MS. They include the use of pure standards, matrix-matched standards, or relevant certified reference materials, assuring traceability of the reported results. This review critically evaluates the advantages and limitations of different calibration approaches, which are used in quantitative analyses by ICP-MS. Examples of such analyses are provided.This article is part of the themed issue 'Quantitative mass spectrometry'.
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http://dx.doi.org/10.1098/rsta.2015.0369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031635PMC
October 2016

Ankle Movements During Supine Kicking in Infants Born Preterm.

Pediatr Phys Ther 2016 ;28(3):294-302

Department of Physical Therapy (Dr Grant-Beuttler), Crean College of Health and Behavioral Science, Chapman University, Irvine, California; Doctoral Programs in Pediatric Science (Dr Heriza), Rocky Mountain University of Health Professions, Provo, Utah; Department of Physical Therapy and Rehabilitation Sciences (Dr Palisano), Drexel University, Philadelphia, Pennsylvania; Department of Physical Therapy (Drs Reddien Wagner and Miller), Panuska College of Professional Studies, University of Scranton, Scranton, Pennsylvania; Department of Human Physiology (Dr Karduna), University of Oregon, Eugene, Oregon.

Background: Knowledge of musculoskeletal factors that influence supine kicking of infants born preterm has implications for early intervention.

Hypotheses: Differences exist between infants born preterm and full-term in ankle kinematics during supine kicking, which are attributable to passive measures of the gastrocnemius/soleus (g/s) muscle tendon unit (MTU).

Subjects: Twenty infants born full-term and 22 born preterm were measured at term, 6 weeks, and 12 weeks of age.

Outcome Measures: Ankle kinematics during supine kicking and g/s MTU length.

Results: Infants born preterm demonstrated less dorsiflexion, more plantar flexion, and more total ankle range during supine kicking. Gestational age explained 69% to 85% of the variability in MTU length from term to 12 weeks of age. MTU lengths explained 0% to 42% of the variance in ankle kinematics.

Conclusions: Passive measures of the g/s MTU may inform clinicians about ankle kinematics in newborns to 12-week infants during supine kicking.
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http://dx.doi.org/10.1097/PEP.0000000000000252DOI Listing
February 2017

Increased microcirculation detected by dynamic contrast-enhanced magnetic resonance imaging is of prognostic significance in asymptomatic myeloma.

Br J Haematol 2016 07 15;174(1):127-35. Epub 2016 Mar 15.

Department of Radiology, German Cancer Research Centre, Heidelberg, Germany.

This prospective study aimed to investigate the prognostic significance of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) as a non-invasive imaging technique delivering the quantitative parameters amplitude A (reflecting blood volume) and exchange rate constant kep (reflecting vascular permeability) in patients with asymptomatic monoclonal plasma cell diseases. We analysed DCE-MRI parameters in 33 healthy controls and 148 patients with monoclonal gammopathy of undetermined significance (MGUS) or smouldering multiple myeloma (SMM) according to the 2003 IMWG guidelines. All individuals underwent standardized DCE-MRI of the lumbar spine. Regions of interest were drawn manually on T1-weighted images encompassing the bone marrow of each of the 5 lumbar vertebrae sparing the vertebral vessel. Prognostic significance for median of amplitude A (univariate: P < 0·001, hazard ratio (HR) 2·42, multivariate P = 0·02, HR 2·7) and exchange rate constant kep (univariate P = 0·03, HR 1·92, multivariate P = 0·46, HR 1·5) for time to progression of 79 patients with SMM was found. Patients with amplitude A above the optimal cut-off point of 0·89 arbitrary units had a 2-year progression rate into symptomatic disease of 80%. In conclusion, DCE-MRI parameters are of prognostic significance for time to progression in patients with SMM but not in individuals with MGUS.
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http://dx.doi.org/10.1111/bjh.14038DOI Listing
July 2016

Association between magnetic resonance imaging patterns and baseline disease features in multiple myeloma: analyzing surrogates of tumour mass and biology.

Eur Radiol 2016 Nov 15;26(11):3939-3948. Epub 2016 Jan 15.

Department of Internal Medicine V, University Hospital Heidelberg, Im Neuenheimer Feld 410, 69120, Heidelberg, Germany.

Objective: To assess associations between bone marrow infiltration patterns and localization in magnetic resonance imaging (MRI) and baseline clinical/prognostic parameters in multiple myeloma (MM).

Methods: We compared baseline MM parameters, MRI patterns and localization of focal lesions to the mineralized bone in 206 newly diagnosed MM patients.

Results: A high tumour mass (represented by International Staging System stage III) was significantly associated with severe diffuse infiltration (p = 0.015) and a higher number of focal lesions (p = 0.006). Elevated creatinine (p = 0.003), anaemia (p < 0.001) and high LDH (p = 0.001) correlated with severe diffuse infiltration. A salt and pepper diffuse pattern had a favourable prognosis. A higher degree of destruction of mineralized bone (assessed by X-ray or computed tomography) was associated with an increasing number of focal lesions on MRI (p < 0.001). Adverse cytogenetics (del17p/gain1q21/t(4;14)) were associated with diffuse infiltration (p = 0.008). The presence of intraosseous focal lesions exceeding the mineralized bone had a borderline significant impact on prognosis.

Conclusions: Diffuse bone marrow infiltration on MRI correlates with adverse cytogenetics, lowered haemoglobin values and high tumour burden in newly diagnosed MM whereas an increasing number of focal lesions correlates with a higher degree of bone destruction. Focal lesions exceeding the cortical bone did not adversely affect the prognosis.

Key Points: • Diffuse MRI correlates with adverse cytogenetics, lowered haemoglobin and high tumour burden. • Higher numbers of MRI focal lesions correlate with increasing degree of bone destruction. • Focal lesions exceeding the cortical bone borderline significantly influence survival. • Moderate/severe diffuse infiltration and more than 23 focal lesions adversely affect survival.
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http://dx.doi.org/10.1007/s00330-015-4195-0DOI Listing
November 2016

Prognostic significance of increased bone marrow microcirculation in newly diagnosed multiple myeloma: results of a prospective DCE-MRI study.

Eur Radiol 2016 May 29;26(5):1404-11. Epub 2015 Jul 29.

German Cancer Research Center, Department of Radiology, Heidelberg, Germany.

Objectives: Aim of this prospective study was to investigate prognostic significance of increased bone marrow microcirculation as detected by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for survival and local complications in patients with multiple myeloma (MM).

Methods: We performed DCE-MRI of the lumbar spine in 131 patients with newly diagnosed MM and analysed data according to the Brix model to acquire amplitude A and exchange rate constant kep. In 61 patients a second MRI performed after therapy was evaluated to assess changes in vertebral height and identify vertebral fractures.

Results: Correlation analysis revealed significant positive association between beta2-microglobulin as well as immunoparesis with DCE-MRI parameters A and kep. Additionally, A was negatively correlated with haemoglobin levels and kep was positively correlated with LDH levels. Higher baseline kep values were associated with decreased vertebral height in a second MRI (P = 0.007) and A values were associated with new vertebral fractures in the lower lumbar spine (P = 0.03 for L4). Pre-existing lytic bone lesions or remission after therapy had no impact on the occurrence of vertebral fractures. Multivariate analysis revealed that amplitude A is an independent adverse risk factor for overall survival.

Conclusion: DCE-MRI is a non-invasive tool with significance for systemic prognosis and vertebral complications.

Key Points: • Qualitative parameters from DCE-MRI are correlated with established factors of disease activity • Increased marrow microcirculation might be a risk factor for loss of vertebral height and fractures • Amplitude A is an independent predictor for shortened overall survival.
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http://dx.doi.org/10.1007/s00330-015-3928-4DOI Listing
May 2016

Assembling Paramagnetic Ceruloplasmin at Electrode Surfaces Covered with Ferromagnetic Nanoparticles. Scanning Electrochemical Microscopy in the Presence of a Magnetic Field.

Langmuir 2015 Jul 17;31(29):8176-83. Epub 2015 Jul 17.

†Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, PL-02-093 Warsaw, Poland.

Adsorption of ceruloplasmin (Cp) at a gold electrode modified with ferromagnetic iron nanoparticles encapsulated in carbon ([email protected] Nps) leads to a successful immobilization of the enzyme in its electroactive form. The proper placement of Cp at the electrode surface on top of the nanocapsules containing an iron core allowed a preorientation of the enzyme, hence allowing direct electron transfer between the electrode and the enzyme. Laser ablation coupled with inductively coupled plasma mass spectrometry indicated that Cp was predominantly located at the paramagnetic nanoparticles. Scanning electrochemical microscopy measurements in the sample-generation/tip-collection mode proved that Cp was ferrooxidative inactive if it was immobilized on the bare gold surface and reached the highest activity if it was adsorbed on [email protected] Nps in the presence of a magnetic field.
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http://dx.doi.org/10.1021/acs.langmuir.5b01155DOI Listing
July 2015

A Branch-and-Bound Approach for Tautomer Enumeration.

Mol Inform 2015 05 7;34(5):263-75. Epub 2015 May 7.

UFZ Department of Ecological Chemistry, Helmholtz Centre for Environmental Research, Permoserstrasse 15, 04318 Leipzig, Germany.

Knowledge about tautomer forms of a structure is important since, e.g., a property prediction for a molecule can yield to different results which depend on the individual tautomer. Tautomers are isomers that can be transformed to each other through chemical equilibrium reactions. In this paper the first exact Branch-and-Bound (B&B) algorithm to calculate tautomer structures is proposed. The algorithm is complete in the sense of tautomerism and generates all possible tautomers of a structure according to the tautomer definition, it is initialized with. To be efficient, the algorithm takes advantage of symmetric and formation properties. Some restrictions are used to enable an early pruning of some branches of the B&B tree. This is important, since a simple enumeration strategy would lead to number of candidate tautomers that is exponentially increasing with the number of hydrogen atoms and their attachment sites. The proposed implementation of the B&B algorithm covers the majority of the prototropic tautomer cases, but can be adapted to other kinds of tautomerism too. Furthermore, a computer processable definition of tautomerism is given in the form of the moving hydrogen atom problem.
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http://dx.doi.org/10.1002/minf.201400128DOI Listing
May 2015

A magnetic resonance imaging-based prognostic scoring system to predict outcome in transplant-eligible patients with multiple myeloma.

Haematologica 2015 Jun 20;100(6):818-25. Epub 2015 Mar 20.

Department of Internal Medicine V, University Hospital of Heidelberg, Heidelberg, Germany

Diffuse and focal bone marrow infiltration patterns detected by magnetic resonance imaging have been shown to be of prognostic significance in all stages of monoclonal plasma cell disorders and have, therefore, been incorporated into the definition of the disease. The aim of this retrospective analysis was to develop a rapidly evaluable prognostic scoring system, incorporating the most significant information acquired from magnetic resonance imaging. Therefore, the impact of bone marrow infiltration patterns on progression-free and overall survival in 161 transplant-eligible myeloma patients was evaluated. Compared to salt and pepper/minimal diffuse infiltration, moderate/severe diffuse infiltration had a negative prognostic impact on both progression-free survival (P<0.001) and overall survival (P=0.003). More than 25 focal lesions on whole-body magnetic resonance imaging or more than seven on axial magnetic resonance imaging were associated with an adverse prognosis (progression-free survival: P=0.001/0.003 and overall survival: P=0.04/0.02). A magnetic resonance imaging-based prognostic scoring system, combining grouped diffuse and focal infiltration patterns, was formulated and is applicable to whole-body as well as axial magnetic resonance imaging. The score identified high-risk patients with median progression-free and overall survival of 23.4 and 55.9 months, respectively (whole-body-based). Multivariate analyses demonstrated that the magnetic resonance imaging-based prognostic score stage III (high-risk) and adverse cytogenetics are independent prognostic factors for both progression-free and overall survival (whole-body-based, progression-free survival: hazard ratio=3.65, P<0.001; overall survival: hazard ratio=5.19, P=0.005). In conclusion, we suggest a magnetic resonance imaging-based prognostic scoring system which is a robust, easy to assess and interpret parameter summarizing significant magnetic resonance imaging findings in transplant-eligible patients with multiple myeloma.
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http://dx.doi.org/10.3324/haematol.2015.124115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450628PMC
June 2015

Dynamic contrast-enhanced magnetic resonance imaging for assessment of antiangiogenic treatment effects in multiple myeloma.

Clin Cancer Res 2015 Jan 28;21(1):106-12. Epub 2014 Oct 28.

Department of Hematology and Oncology, University Hospital of Heidelberg, Heidelberg, Germany. Department of Radiology, German Cancer Research Center, Heidelberg, Germany.

Purpose: To noninvasively assess bone marrow microcirculation before and after therapy in patients with newly diagnosed multiple myeloma with dynamic contrast-enhanced MRI (DCE-MRI).

Experimental Design: Ninety-six patients received DCE-MRI before and after primary treatment for newly diagnosed multiple myeloma. For the 91 evaluable patients, treatment consisted of high-dose therapy (HDT) with autologous stem cell transplantation (ASCT) in 82 patients and chemotherapy without ASCT in 9 patients. In addition, 33 healthy volunteers were imaged as the control group. Analysis of DCE-MRI was performed according to the two-compartment model by Brix to quantify amplitude A (associated with blood volume) and exchange rate constant kep (reflecting vessel permeability and perfusion).

Results: Nonresponders showed significantly higher A-values before the start of therapy compared with responders (P = 0.02). In both responders and nonresponders to therapy, A-values dropped significantly (P = 0.004 and <0.001, respectively) after primary therapy, whereas lower values for kep were found only in responders (P < 0.001). Depth of remission was significantly correlated to decreased bone marrow microcirculation: Patients in near complete response (nCR) or complete remission (CR) after treatment showed significantly lower values for A compared with patients not achieving nCR+CR. The application of HDT or novel agents had no significant effect on DCE-MRI parameters after therapy, although patients treated with novel agents more often achieved nCR+CR (42%/12.5%; P < 0.002). Higher kep-values at second MRI were positively correlated to shorter overall survival (HR 3.53; 95% confidence intervals, 1.21-10.33; P = 0.02).

Conclusion: Parameters from DCE-MRI are correlated to remission after primary therapy and outcome in newly diagnosed multiple myeloma.
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http://dx.doi.org/10.1158/1078-0432.CCR-14-1029DOI Listing
January 2015

Influence of slip on the Rayleigh-Plateau rim instability in dewetting viscous films.

Phys Rev Lett 2014 Jul 2;113(1):014501. Epub 2014 Jul 2.

Saarland University, Department of Experimental Physics, Campus, D-66123 Saarbrücken, Germany.

A dewetting viscous film develops a characteristic fluid rim at its receding edge due to mass conservation. In the course of the dewetting process, the rim becomes unstable via an instability of Rayleigh-Plateau type. An important difference exists between this classic instability of a liquid column and the rim instability in a thin film as the growth of the rim is continuously fueled by the receding film. We explain how the development and macroscopic morphology of the rim instability are controlled by the slip of the film on the substrate. A single thin-film model captures quantitatively the characteristics of the complete evolution of the rim observed in the experiments.
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http://dx.doi.org/10.1103/PhysRevLett.113.014501DOI Listing
July 2014
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