Publications by authors named "Barbara Madej-Czerwonka"

5 Publications

  • Page 1 of 1

The Correlation of Mutations and Expressions of Genes within the PI3K/Akt/mTOR Pathway in Breast Cancer-A Preliminary Study.

Int J Mol Sci 2021 Feb 19;22(4). Epub 2021 Feb 19.

Chair and Department of Biology and Genetics, Medical University of Lublin, 20-093 Lublin, Poland.

There is an urgent need to seek new molecular biomarkers helpful in diagnosing and treating breast cancer. In this elaboration, we performed a molecular analysis of mutations and expression of genes within the PI3K/Akt/mTOR pathway in patients with ductal breast cancer of various malignancy levels. We recognized significant correlations between the expression levels of the studied genes. We also performed a bioinformatics analysis of the data available on the international database TCGA and compared them with our own research. Studies on mutations and expression of genes were conducted using High-Resolution Melt PCR (HRM-PCR), Allele-Specific-quantitative PCR (ASP-qPCR), Real-Time PCR molecular methods in a group of women with ductal breast cancer. Bioinformatics analysis was carried out using web source Ualcan and bc-GenExMiner. In the studied group of women, it was observed that the prevalence of mutations in the studied and genes was 29.63%. It was stated that the average expression level of the , , genes in the group of breast cancer patients is lower in comparison to the control group, while the average expression level of the and genes in the studied group was higher in comparison to the control group. It was also indicated that in the group of patients with mutations in the area of the and genes, the gene expression level is statistically significantly lower than in the group without mutations. According to our knowledge, we demonstrate, for the first time, that there is a very strong positive correlation between the levels of and gene expression in the case of patients with mutations and without mutations.
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http://dx.doi.org/10.3390/ijms22042061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922286PMC
February 2021

Analysis of the influence of parenteral cancer chemotherapy on the health condition of oral mucosa.

Contemp Oncol (Pozn) 2015 26;19(1):77-82. Epub 2015 Mar 26.

The Chair and Department of Oral Surgery, Medical University of Lublin, Lublin, Poland.

Aim Of The Study: The present study was aimed at estimating the prevalence of oral complications in cancer patients receiving chemotherapy.

Material And Methods: The study was conducted on a group of 58 patients treated with chemotherapy (study group). The control group consisted of 30 healthy patients. Dental status and oral mucosa were examined using the criteria of the National Cancer Institute Toxicity Criteria Scale. The levels of stimulated and unstimulated saliva flow were analysed.

Results: In the group of patients treated with chemotherapy, 59% of patients had inflammatory changes of the soft tissues of the mouth, such as erythema, erosions, or ulcers, which were discovered during dental examination. Such changes occurred in only 10% of patients in the control group. Six of the patients treated with chemotherapy reported pain with intensity was so severe that it caused swallowing difficulties. Patients in the study group frequently complained about the presence of dry mouth, taste disturbances, nausea, and vomiting. These symptoms occurred in 70% of patients undergoing oncological treatment. In both stimulated and unstimulated saliva secretion, the rates were significantly lower in patients from the research group, when compared to the control group.
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http://dx.doi.org/10.5114/wo.2014.45291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507886PMC
July 2015

Standards in breast surgery - Breast Units - future and necessity.

Contemp Oncol (Pozn) 2014 26;18(4):227-9. Epub 2014 Jun 26.

Department of Breast Surgery, Specialist County Hospital of Stephan Cardinal Wyszyński in Lublin, Medical University of Lublin, Poland.

Breast cancer is the most common type of malignant neoplasm affecting women. In Poland there are nearly 15 500 new cases of the disease and over 5200 deaths due to it observed annually. As it was necessary to provide those suffering from breast cancer with combined highly specialist treatment, it was required that separate units specialising in breast surgery be established. In Europe they are referred to as Breast Units. The idea of forming such separate and fully profiled centres specialising in breast diseases, which was developed in 1998 by EORTC, EUSOMA as well as Europa Donna, gave foundations for the organisation of such centres. The situation of Breast Units in other European countries is different. Poland has failed so far to organise an effective system of Breast Units. Leading Polish authorities underline that formation of multi-disciplinary Breast Units is the organisational priority for the year 2013.
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http://dx.doi.org/10.5114/wo.2014.43489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171469PMC
September 2014

Polish Code of Ethics of a Medical Laboratory Specialist.

EJIFCC 2014 Sep 4;25(2):199-206. Epub 2014 Sep 4.

Medical University of Lublin , Department of Descriptive and Clinical Anatomy.

Along with the development of medicine, increasingly significant role has been played by the laboratory diagnostics. For over ten years the profession of the medical laboratory specialist has been regarded in Poland as the autonomous medical profession and has enjoyed a status of one of public trust. The process of education of medical laboratory specialists consists of a five-year degree in laboratory medicine, offered at Medical Universities, and of a five-year Vocational Specialization in one of the fields of laboratory medicine such as clinical biochemistry, medical microbiology, medical laboratory toxicology, medical laboratory cytomorphology and medical laboratory transfusiology. An important component of medical laboratory specialists' identity is awareness of inherited ethos obtained from bygone generations of workers in this particular profession and the need to continue its further development. An expression of this awareness is among others Polish Code of Ethics of a Medical Laboratory Specialist (CEMLS) containing a set of values and a moral standpoint characteristic of this type of professional environment. Presenting the ethos of the medical laboratory specialist is a purpose of this article. Authors focus on the role CEMLS plays in areas of professional ethics and law. Next, they reconstruct the Polish model of ethos of medical diagnostic laboratory personnel. An overall picture consists of a presentation of the general moral principles concerning execution of this profession and rules of conduct in relations with the patient, own professional environment and the rest of the society. Polish model of ethical conduct, which is rooted in Hippocratic medical tradition, harmonizes with the ethos of medical laboratory specialists of other European countries and the world.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4975296PMC
September 2014

Intensification of doxorubicin-related oxidative stress in the heart by hypothyroidism is not related to the expression of cytochrome P450 NADPH-reductase and inducible nitric oxide synthase, as well as activity of xanthine oxidase.

Oxid Med Cell Longev 2012 23;2012:139327. Epub 2012 Aug 23.

Medical Biology Unit, Medical University of Lublin, 20-059 Lublin, Poland.

Cytochrome P450 NADPH-reductase (P450R), inducible synthase (iNOS) and xanthine oxidase play an important role in the antracycline-related cardiotoxicity. The expression of P450R and iNOS is regulated by triiodothyronine. The aim of this study was to evaluate the effect of methimazole-induced hypothyreosis on oxidative stress secondary to doxorubicin administration. 48 hours after methimazole giving cessation, rats were exposed to doxorubicin (2.0, 5.0 and 15 mg/kg). Blood and heart were collected 4, 48 and 96 h after the drug administration. Animals exposed exclusively to doxorubicin or untreated ones were also assessed. The hypothyreosis (0.025% of methimazole) significantly increased the doxorubicin effect on the cardiac carbonyl group and they may increase the glutathione level. An insignificant effect of methimazole was noticed in case of the cardiac lipid peroxidation product, the amount of DNA oxidative damages, iNOS and xanthine oxidase-enzymes responsible for red-ox activation of doxorubicin. However, the concentration of P450R was affected by a lower dose of methimazole in rats administered with doxorubicin. Since in rats receiving doxorubicin changes in oxidative stress caused by methimazole were not accompanied by elevation of bioreductive enzymes, it may be concluded that these changes in the oxidative stress were not related to the tested enzymes.
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http://dx.doi.org/10.1155/2012/139327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432562PMC
January 2013
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