Barbara Imperiali

Barbara Imperiali

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Barbara Imperiali

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A Strategic Approach for Fluorescence Imaging of Membrane Proteins in a Native-like Environment.

Cell Chem Biol 2020 Feb 9;27(2):245-251.e3. Epub 2019 Dec 9.

Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.chembiol.2019.11.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036013PMC
February 2020

Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution.

J Am Chem Soc 2020 Feb 21;142(5):2145-2149. Epub 2020 Jan 21.

National Synchrotron Light Source II , Brookhaven National Laboratory , Upton , New York 11973 , United States.

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http://dx.doi.org/10.1021/jacs.9b11571DOI Listing
February 2020

Deploying Fluorescent Nucleoside Analogues for High-Throughput Inhibitor Screening.

Chembiochem 2020 Jan 3;21(1-2):108-112. Epub 2019 Dec 3.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

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http://dx.doi.org/10.1002/cbic.201900671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980326PMC
January 2020

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces.

Curr Opin Struct Biol 2019 12 16;59:81-90. Epub 2019 Apr 16.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X193003
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http://dx.doi.org/10.1016/j.sbi.2019.03.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885101PMC
December 2019

Bacterial carbohydrate diversity - a Brave New World.

Curr Opin Chem Biol 2019 12 6;53:1-8. Epub 2019 Jun 6.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.cbpa.2019.04.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893104PMC
December 2019

Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates.

Arch Biochem Biophys 2019 10 26;675:108111. Epub 2019 Sep 26.

Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA, 02139, USA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.abb.2019.108111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909930PMC
October 2019

Preface.

Methods Enzymol 2017 ;597:xv-xix

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http://dx.doi.org/10.1016/S0076-6879(17)30318-XDOI Listing
January 2019

Preface.

Methods Enzymol 2018 ;598:xv-xix

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http://dx.doi.org/10.1016/S0076-6879(17)30417-2DOI Listing
January 2019

Monotopic Membrane Proteins Join the Fold.

Trends Biochem Sci 2019 01 15;44(1):7-20. Epub 2018 Oct 15.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.tibs.2018.09.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309722PMC
January 2019

Design, solid-phase synthesis and evaluation of enterobactin analogs for iron delivery into the human pathogen Campylobacter jejuni.

Bioorg Med Chem 2018 10 16;26(19):5314-5321. Epub 2018 Apr 16.

Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.bmc.2018.04.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191362PMC
October 2018

Facile Solid-Phase Synthesis and Assessment of Nucleoside Analogs as Inhibitors of Bacterial UDP-Sugar Processing Enzymes.

ACS Chem Biol 2018 09 16;13(9):2542-2550. Epub 2018 Aug 16.

Department of Biology , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.

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http://dx.doi.org/10.1021/acschembio.8b00477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457116PMC
September 2018

Membrane association of monotopic phosphoglycosyl transferase underpins function.

Nat Chem Biol 2018 06 16;14(6):538-541. Epub 2018 May 16.

Program in Biomolecular Pharmacology, Boston University School of Medicine, Boston, MA, USA.

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http://www.nature.com/articles/s41589-018-0054-z
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http://dx.doi.org/10.1038/s41589-018-0054-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202225PMC
June 2018

Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.

Methods Enzymol 2017 5;597:145-186. Epub 2017 Jul 5.

Massachusetts Institute of Technology, Cambridge, MA, United States. Electronic address:

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http://dx.doi.org/10.1016/bs.mie.2017.06.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6710627PMC
May 2018

Biogenesis of Asparagine-Linked Glycoproteins Across Domains of Life-Similarities and Differences.

ACS Chem Biol 2018 04 26;13(4):833-837. Epub 2018 Feb 26.

Department of Biology and Department of Chemistry , Massachusetts Institute of Technology , Cambridge , Massachusetts , United States.

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http://dx.doi.org/10.1021/acschembio.8b00163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338551PMC
April 2018

Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases.

Trends Biochem Sci 2018 01 25;43(1):10-17. Epub 2017 Nov 25.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.tibs.2017.10.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741494PMC
January 2018

Bacterial phosphoglycosyl transferases: initiators of glycan biosynthesis at the membrane interface.

Glycobiology 2017 09;27(9):820-833

Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1093/glycob/cwx064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881778PMC
September 2017

Conformational dynamics and alignment properties of loop lanthanide-binding-tags (LBTs) studied in interleukin-1β.

J Biomol NMR 2017 Jul 22;68(3):187-194. Epub 2017 May 22.

Center for Biomolecular Magnetic Resonance, Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University of Frankfurt, Max-von-Laue-Strasse 7, 60438, Frankfurt, Germany.

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http://dx.doi.org/10.1007/s10858-017-0118-5DOI Listing
July 2017

Analysis of a dual domain phosphoglycosyl transferase reveals a ping-pong mechanism with a covalent enzyme intermediate.

Proc Natl Acad Sci U S A 2017 07 19;114(27):7019-7024. Epub 2017 Jun 19.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139;

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http://dx.doi.org/10.1073/pnas.1703397114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502628PMC
July 2017

Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyltransferase from Campylobacter jejuni.

J Med Chem 2017 03 22;60(5):2099-2118. Epub 2017 Feb 22.

Department of Chemistry, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/acs.jmedchem.6b01869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506375PMC
March 2017

Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.

J Mol Biol 2016 10 21;428(20):4228-4241. Epub 2016 Jul 21.

Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Koch Institute for Integrative Cancer Research, 500 Main Street, Cambridge, MA 02139, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address:

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048519PMC
http://dx.doi.org/10.1016/j.jmb.2016.07.004DOI Listing
October 2016

Bacterial N-Glycosylation Efficiency Is Dependent on the Structural Context of Target Sequons.

J Biol Chem 2016 Oct 29;291(42):22001-22010. Epub 2016 Aug 29.

From the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

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http://dx.doi.org/10.1074/jbc.M116.747121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063983PMC
October 2016

A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes.

Sci Rep 2016 09 14;6:33412. Epub 2016 Sep 14.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1038/srep33412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022061PMC
September 2016

N-Linked Glycans Are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus.

PLoS One 2015 22;10(6):e0130482. Epub 2015 Jun 22.

Department of Biochemistry, Duke University Medical Center, Durham NC 27710, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130482PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476749PMC
April 2016

A Modular Approach to Phosphoglycosyltransferase Inhibitors Inspired by Nucleoside Antibiotics.

Chemistry 2016 Mar 10;22(11):3856-64. Epub 2015 Dec 10.

Departments of Chemistry and Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

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http://dx.doi.org/10.1002/chem.201503986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506376PMC
March 2016

Probing Polytopic Membrane Protein-Substrate Interactions by Luminescence Resonance Energy Transfer.

J Am Chem Soc 2016 Mar 14;138(11):3806-12. Epub 2016 Mar 14.

Departments of Biology and Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/jacs.5b13426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480290PMC
March 2016

Conservation and Covariance in Small Bacterial Phosphoglycosyltransferases Identify the Functional Catalytic Core.

Biochemistry 2015 Dec 4;54(50):7326-34. Epub 2015 Dec 4.

Departments of Chemistry and Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/acs.biochem.5b01086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483379PMC
December 2015

Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.

J Biomol NMR 2015 Nov 4;63(3):275-82. Epub 2015 Sep 4.

Institute of Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Straße 7, 60438, Frankfurt Am Main, Germany.

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http://dx.doi.org/10.1007/s10858-015-9984-xDOI Listing
November 2015

Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling.

J Am Chem Soc 2015 Oct 23;137(39):12446-9. Epub 2015 Sep 23.

Departments of Biology and Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/jacs.5b07146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599313PMC
October 2015

The best and the brightest: exploiting tryptophan-sensitized Tb(3+) luminescence to engineer lanthanide-binding tags.

Methods Mol Biol 2015 ;1248:201-20

Department of Chemistry and Physics, Warren Wilson College, Asheville, NC, USA.

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http://dx.doi.org/10.1007/978-1-4939-2020-4_14DOI Listing
September 2015

Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.

Biomacromolecules 2015 Aug 10;16(8):2316-26. Epub 2015 Jul 10.

†Department of Biological Engineering, ‡Department of Chemical Engineering, §Center for Gynepathology Research, ∥Department of Chemistry and ⊥̂Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts United States.

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http://dx.doi.org/10.1021/acs.biomac.5b00549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613866PMC
August 2015

Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates.

Glycobiology 2015 Jul 11;25(7):756-66. Epub 2015 Mar 11.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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http://dx.doi.org/10.1093/glycob/cwv016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453864PMC
July 2015

Equilibrium and dynamic design principles for binding molecules engineered for reagentless biosensors.

Anal Biochem 2014 Sep 9;460:9-15. Epub 2014 May 9.

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ab.2014.04.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4104283PMC
September 2014

Selective mitogen activated protein kinase activity sensors through the application of directionally programmable D domain motifs.

Biochemistry 2014 Sep 3;53(36):5771-8. Epub 2014 Sep 3.

Departments of Chemistry and Biology, and ‡Departments of Biology and Biological Engineering, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/bi500862cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4165445PMC
September 2014

Quantification of protein kinase enzymatic activity in unfractionated cell lysates using CSox-based sensors.

Curr Protoc Chem Biol 2014 Sep 9;6(3):135-156. Epub 2014 Sep 9.

Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska.

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http://doi.wiley.com/10.1002/9780470559277.ch140106
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http://dx.doi.org/10.1002/9780470559277.ch140106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174361PMC
September 2014

Tailoring chimeric ligands for studying and biasing ErbB receptor family interactions.

Angew Chem Int Ed Engl 2014 Mar 31;53(10):2662-6. Epub 2014 Jan 31.

Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA (USA).

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http://dx.doi.org/10.1002/anie.201307869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018821PMC
March 2014

The renaissance of bacillosamine and its derivatives: pathway characterization and implications in pathogenicity.

Biochemistry 2014 Feb 21;53(4):624-38. Epub 2014 Jan 21.

Departments of Chemistry and Biology, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

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http://pubs.acs.org/doi/10.1021/bi401546r
Publisher Site
http://dx.doi.org/10.1021/bi401546rDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3951908PMC
February 2014

Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates.

J Phys Chem B 2013 Dec 27;117(50):15935-42. Epub 2013 Nov 27.

Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.

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http://dx.doi.org/10.1021/jp407321gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938489PMC
December 2013

Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities.

Proc Natl Acad Sci U S A 2013 Dec 3;110(52):20863-70. Epub 2013 Dec 3.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.

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http://dx.doi.org/10.1073/pnas.1320852110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876266PMC
December 2013

Biochemical analysis and structure determination of bacterial acetyltransferases responsible for the biosynthesis of UDP-N,N'-diacetylbacillosamine.

J Biol Chem 2013 Nov 24;288(45):32248-60. Epub 2013 Sep 24.

From the Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

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http://dx.doi.org/10.1074/jbc.M113.510560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820863PMC
November 2013

Biosynthesis of UDP-N,N'-diacetylbacillosamine in Acinetobacter baumannii: Biochemical characterization and correlation to existing pathways.

Arch Biochem Biophys 2013 Aug 5;536(1):72-80. Epub 2013 Jun 5.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States.

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http://dx.doi.org/10.1016/j.abb.2013.05.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722612PMC
August 2013

Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase.

Protein Expr Purif 2013 Jun 12;89(2):241-50. Epub 2013 Apr 12.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.pep.2013.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690457PMC
June 2013

Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis.

Nat Chem Biol 2013 Jun 28;9(6):367-73. Epub 2013 Apr 28.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nchembio.1249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661703PMC
June 2013

Fluorescent amino acids: modular building blocks for the assembly of new tools for chemical biology.

Chembiochem 2013 May 22;14(7):788-99. Epub 2013 Apr 22.

Department of Biology, Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave. 68-395 Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1002/cbic.201300079DOI Listing
May 2013

Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions.

J Am Chem Soc 2013 Mar 13;135(12):4580-3. Epub 2013 Mar 13.

Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja309870qDOI Listing
March 2013

FRET-capture: a sensitive method for the detection of dynamic protein interactions.

Chembiochem 2013 Jan 13;14(1):53-7. Epub 2012 Dec 13.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1002/cbic.201200700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776414PMC
January 2013

Tailoring encodable lanthanide-binding tags as MRI contrast agents.

Chembiochem 2012 Nov 13;13(17):2567-74. Epub 2012 Nov 13.

Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02218, USA.

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http://dx.doi.org/10.1002/cbic.201200448DOI Listing
November 2012

The chemistry-glycobiology frontier.

J Am Chem Soc 2012 Oct;134(43):17835-9

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http://dx.doi.org/10.1021/ja309542qDOI Listing
October 2012

At the membrane frontier: a prospectus on the remarkable evolutionary conservation of polyprenols and polyprenyl-phosphates.

Arch Biochem Biophys 2012 Jan 10;517(2):83-97. Epub 2011 Nov 10.

Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.abb.2011.10.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253937PMC
January 2012

Chemical tools for studying directed cell migration.

ACS Chem Biol 2011 Nov 20;6(11):1164-74. Epub 2011 Oct 20.

Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/cb200299kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220797PMC
November 2011

Development of a fluorogenic sensor for activated Cdc42.

Bioorg Med Chem Lett 2011 Sep 20;21(17):5058-61. Epub 2011 Apr 20.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.bmcl.2011.04.051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156282PMC
September 2011

Exploiting topological constraints to reveal buried sequence motifs in the membrane-bound N-linked oligosaccharyl transferases.

Biochemistry 2011 Sep 16;50(35):7557-67. Epub 2011 Aug 16.

Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1021/bi201018dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164389PMC
September 2011

Sequential activation and deactivation of protein function using spectrally differentiated caged phosphoamino acids.

J Am Chem Soc 2011 Jul 29;133(29):11038-41. Epub 2011 Jun 29.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/ja2028074
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http://dx.doi.org/10.1021/ja2028074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146339PMC
July 2011

Light-triggered myosin activation for probing dynamic cellular processes.

Angew Chem Int Ed Engl 2011 Jun 3;50(25):5667-70. Epub 2011 May 3.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1002/anie.201100674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406609PMC
June 2011

Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system.

Proc Natl Acad Sci U S A 2011 Jun 23;108(23):9643-8. Epub 2011 May 23.

Center for Molecular Biology and Neuroscience and Department of Molecular Biosciences, University of Oslo, 0316 Oslo, Norway.

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http://dx.doi.org/10.1073/pnas.1103321108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3111294PMC
June 2011

The expanding horizons of asparagine-linked glycosylation.

Biochemistry 2011 May 4;50(21):4411-26. Epub 2011 May 4.

Department of Chemistry Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bi200346n
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http://dx.doi.org/10.1021/bi200346nDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101296PMC
May 2011

Engineering encodable lanthanide-binding tags into loop regions of proteins.

J Am Chem Soc 2011 Feb;133(4):808-19

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University of Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt, Germany.

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http://dx.doi.org/10.1021/ja104983tDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3043167PMC
February 2011

Biomimetic divalent ligands for the acute disruption of synaptic AMPAR stabilization.

Nat Chem Biol 2011 Feb 26;7(2):81-91. Epub 2010 Dec 26.

Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nchembio.498DOI Listing
February 2011

A p38α-selective chemosensor for use in unfractionated cell lysates.

ACS Chem Biol 2011 Jan 30;6(1):101-5. Epub 2010 Sep 30.

Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, 02139, United States.

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http://dx.doi.org/10.1021/cb100230yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025060PMC
January 2011

Development of a multicomponent kinetic assay of the early enzymes in the Campylobacter jejuni N-linked glycosylation pathway.

Bioorg Med Chem 2010 Dec 29;18(23):8167-71. Epub 2010 Oct 29.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, 02139, United States.

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http://dx.doi.org/10.1016/j.bmc.2010.10.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981441PMC
December 2010

Perturbing the folding energy landscape of the bacterial immunity protein Im7 by site-specific N-linked glycosylation.

Proc Natl Acad Sci U S A 2010 Dec 9;107(52):22528-33. Epub 2010 Dec 9.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1073/pnas.1015356107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012502PMC
December 2010

Dynamic and specific interaction between synaptic NR2-NMDA receptor and PDZ proteins.

Proc Natl Acad Sci U S A 2010 Nov 25;107(45):19561-6. Epub 2010 Oct 25.

Laboratory for Cellular Physiology of the Synapse, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5091, 33077 Bordeaux, France.

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http://dx.doi.org/10.1073/pnas.1002690107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984211PMC
November 2010

Modulation of Shank3 PDZ domain ligand-binding affinity by dimerization.

Chembiochem 2010 Sep;11(14):1979-84

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA.

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http://dx.doi.org/10.1002/cbic.201000246DOI Listing
September 2010

Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly.

Biochemistry 2010 Aug;49(33):7227-37

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bi100805b
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923267PMC
August 2010

Lanthanide-tagged proteins--an illuminating partnership.

Curr Opin Chem Biol 2010 Apr 25;14(2):247-54. Epub 2010 Jan 25.

Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, MA 02215-2521, USA.

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http://dx.doi.org/10.1016/j.cbpa.2010.01.004DOI Listing
April 2010

Monitoring protein interactions and dynamics with solvatochromic fluorophores.

Trends Biotechnol 2010 Feb 3;28(2):73-83. Epub 2009 Dec 3.

Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1016/j.tibtech.2009.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2818466PMC
February 2010

Monitoring protein kinases in cellular media with highly selective chimeric reporters.

Angew Chem Int Ed Engl 2009 ;48(37):6828-31

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA, USA.

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http://dx.doi.org/10.1002/anie.200902374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2937354PMC
December 2009

Thiol-reactive derivatives of the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore: a highly sensitive toolset for the detection of biomolecular interactions.

Bioconjug Chem 2009 Nov;20(11):2133-41

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bc900319z
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783863PMC
November 2009

Synthesis of red-shifted 8-hydroxyquinoline derivatives using click chemistry and their incorporation into phosphorylation chemosensors.

J Org Chem 2009 Oct;74(19):7309-14

Department of Chemistry, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/jo901369kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762270PMC
October 2009

Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1.

Biochemistry 2009 Jun;48(23):5446-55

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge,Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/bi900186uDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694220PMC
June 2009

A general screening strategy for peptide-based fluorogenic ligands: probes for dynamic studies of PDZ domain-mediated interactions.

J Am Chem Soc 2009 May;131(19):6680-2

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://pubs.acs.org/doi/abs/10.1021/ja900371q
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828873PMC
May 2009

Interrogating biology with a chemical lexicon.

Nat Chem 2009 Apr;1(1):9-10

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1038/nchem.144DOI Listing
April 2009

Campylobacter jejuni PglH is a single active site processive polymerase that utilizes product inhibition to limit sequential glycosyl transfer reactions.

Biochemistry 2009 Mar;48(12):2807-16

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/bi802284dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2736683PMC
March 2009

A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.

Bioorg Med Chem Lett 2009 Feb 27;19(4):1258-60. Epub 2008 Dec 27.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.bmcl.2008.12.090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838374PMC
February 2009

Crystal structure and catalytic mechanism of PglD from Campylobacter jejuni.

J Biol Chem 2008 Oct 30;283(41):27937-46. Epub 2008 Jul 30.

Department of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://www.jbc.org/lookup/doi/10.1074/jbc.M801207200
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562079PMC
October 2008

A versatile amino acid analogue of the solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide: a powerful tool for the study of dynamic protein interactions.

J Am Chem Soc 2008 Oct 23;130(41):13630-8. Epub 2008 Sep 23.

Department of Chemistry, Massachusetts Institute of Technology, 77 Mass Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja804754yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647014PMC
October 2008

Recognition-domain focused chemosensors: versatile and efficient reporters of protein kinase activity.

J Am Chem Soc 2008 Sep 29;130(38):12821-7. Epub 2008 Aug 29.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja8046188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637182PMC
September 2008

Structure determination of a Galectin-3-carbohydrate complex using paramagnetism-based NMR constraints.

Protein Sci 2008 Jul 15;17(7):1220-31. Epub 2008 Apr 15.

Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA.

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http://dx.doi.org/10.1110/ps.034561.108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442008PMC
July 2008

Chemoenzymatic synthesis of polyprenyl phosphates.

Bioorg Med Chem 2008 May 14;16(9):5149-56. Epub 2008 Mar 14.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1016/j.bmc.2008.03.025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2471869PMC
May 2008

Lanthanide-binding tags with unnatural amino acids: sensitizing Tb3+ and Eu3+ luminescence at longer wavelengths.

Bioconjug Chem 2008 Mar 15;19(3):588-91. Epub 2008 Feb 15.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1021/bc700426cDOI Listing
March 2008

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization.

Nat Protoc 2007 ;2(12):3201-9

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://www.nature.com/articles/nprot.2007.442
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February 2008

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach.

Nat Protoc 2007 ;2(12):3210-8

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1038/nprot.2007.443DOI Listing
February 2008

Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN.

Nat Protoc 2007 ;2(12):3219-25

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

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http://dx.doi.org/10.1038/nprot.2007.444DOI Listing
February 2008

Polyisoprenol specificity in the Campylobacter jejuni N-linked glycosylation pathway.

Biochemistry 2007 Dec 23;46(50):14342-8. Epub 2007 Nov 23.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bi701956x
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585822PMC
December 2007

Caged O-phosphorothioyl amino acids as building blocks for Fmoc-based solid phase peptide synthesis.

Tetrahedron 2007 Jul;63(27):6185-6190

Massachusetts Institute of Technology, Department of Chemistry, 18-590, 77 Massachusetts Ave., Cambridge, MA 02139, USA.

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http://linkinghub.elsevier.com/retrieve/pii/S004040200700398
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http://dx.doi.org/10.1016/j.tet.2007.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699315PMC
July 2007

Double-lanthanide-binding tags: design, photophysical properties, and NMR applications.

J Am Chem Soc 2007 Jun 12;129(22):7106-13. Epub 2007 May 12.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1021/ja070480vDOI Listing
June 2007

Double-lanthanide-binding tags for macromolecular crystallographic structure determination.

J Am Chem Soc 2007 Jun 12;129(22):7114-20. Epub 2007 May 12.

Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA.

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http://dx.doi.org/10.1021/ja070481nDOI Listing
June 2007

From peptide to protein: comparative analysis of the substrate specificity of N-linked glycosylation in C. jejuni.

Biochemistry 2007 May 17;46(18):5579-85. Epub 2007 Apr 17.

Department of Chemistry, Massachusetts Institute of Technology 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

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http://pubs.acs.org/doi/abs/10.1021/bi602633n
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May 2007

Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells.

Nat Chem Biol 2007 Apr 11;3(4):222-8. Epub 2007 Mar 11.

Department of Pathology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, Massachusetts 01655, USA.

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http://dx.doi.org/10.1038/nchembio868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444530PMC
April 2007

Semisynthesis of unnatural amino acid mutants of paxillin: protein probes for cell migration studies.

Protein Sci 2007 Mar 22;16(3):550-6. Epub 2007 Jan 22.

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1110/ps.062549407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203312PMC
March 2007

Design, synthesis, and characterization of caged compounds.

CSH Protoc 2007 Jan 1;2007:pdb.ip25. Epub 2007 Jan 1.

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http://dx.doi.org/10.1101/pdb.ip25DOI Listing
January 2007