Publications by authors named "Baohua Wang"

113 Publications

MoCpa1-mediated arginine biosynthesis is crucial for fungal growth, conidiation, and plant infection of Magnaporthe oryzae.

Appl Microbiol Biotechnol 2021 Jul 22. Epub 2021 Jul 22.

Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Arginine is an important amino acid involved in processes such as cell signal transduction, protein synthesis, and sexual reproduction. To understand the biological roles of arginine biosynthesis in pathogenic fungi, we used Cpa1, the carbamoyl phosphate synthase arginine-specific small chain subunit in Saccharomyces cerevisiae as a query to identify its ortholog in the Magnaporthe oryzae genome and named it MoCpa1. MoCpa1 is a 471-amino acid protein containing a CPSase_sm_chain domain and a GATase domain. MoCpa1 transcripts were highly expressed at the conidiation, early-infection, and late-infection stages of the fungus. Targeted deletion of the MoCPA1 gene resulted in a ΔMocpa1 mutant exhibiting arginine auxotrophy on minimum culture medium (MM), confirming its role in de novo arginine biosynthesis. The ΔMocpa1 mutant presented significantly decreased sporulation with some of its conidia being defective in morphology. Furthermore, the ΔMocpa1 mutant was nonpathogenic on rice and barley leaves, which was a result of defects in appressorium-mediated penetration and restricted invasive hyphal growth within host cells. Addition of exogenous arginine partially rescued conidiation and pathogenicity defects on the barley and rice leaves, while introduction of the MoCPA1 gene into the ΔMocpa1 mutant fully complemented the lost phenotype. Further confocal microscopy examination revealed that MoCpa1 is localized in the mitochondria. In summary, our results demonstrate that MoCpa1-mediated arginine biosynthesis is crucial for fungal development, conidiation, appressorium formation, and infection-related morphogenesis in M. oryzae, thus serving as an attractive target for mitigating obstinate fungal plant pathogens. KEY POINTS: • MoCpa1 is important for aerial hyphal growth and arginine biosynthesis. • MoCpa1 is pivotal for conidial morphogenesis and appressorium formation. • MoCpa1 is crucial for full virulence in M. oryzae.
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http://dx.doi.org/10.1007/s00253-021-11437-1DOI Listing
July 2021

Genetic basis of maize kernel oil-related traits revealed by high-density SNP markers in a recombinant inbred line population.

BMC Plant Biol 2021 Jul 21;21(1):344. Epub 2021 Jul 21.

Ministry of Agricultural Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region, School of Life Sciences, Nantong University, Nantong, 226019, People's Republic of China.

Background: Maize (Zea mays ssp. mays) is the most abundantly cultivated and highly valued food commodity in the world. Oil from maize kernels is highly nutritious and important for the diet and health of humans, and it can be used as a source of bioenergy. A better understanding of genetic basis for maize kernel oil can help improve the oil content and quality when applied in breeding.

Results: In this study, a KUI3/SC55 recombinant inbred line (RIL) population, consisting of 180 individuals was constructed from a cross between inbred lines KUI3 and SC55. We phenotyped 19 oil-related traits and subsequently dissected the genetic architecture of oil-related traits in maize kernels based on a high-density genetic map. In total, 62 quantitative trait loci (QTLs), with 2 to 5 QTLs per trait, were detected in the KUI3/SC55 RIL population. Each QTL accounted for 6.7% (qSTOL1) to 31.02% (qBELI6) of phenotypic variation and the total phenotypic variation explained (PVE) of all detected QTLs for each trait ranged from 12.5% (OIL) to 52.5% (C16:0/C16:1). Of all these identified QTLs, only 5 were major QTLs located in three genomic regions on chromosome 6 and 9. In addition, two pairs of epistatic QTLs with additive effects were detected and they explained 3.3 and 2.4% of the phenotypic variation, respectively. Colocalization with a previous GWAS on oil-related traits, identified 19 genes. Of these genes, two important candidate genes, GRMZM2G101515 and GRMZM2G022558, were further verified to be associated with C20:0/C22:0 and C18:0/C20:0, respectively, according to a gene-based association analysis. The first gene encodes a kinase-related protein with unknown function, while the second gene encodes fatty acid elongase 2 (fae2) and directly participates in the biosynthesis of very long chain fatty acids in Arabidopsis.

Conclusions: Our results provide insights on the genetic basis of oil-related traits and a theoretical basis for improving maize quality by marker-assisted selection.
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http://dx.doi.org/10.1186/s12870-021-03089-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293480PMC
July 2021

Transcriptome analysis reveals genes potentially related to high fiber strength in a line IL9 with introgression.

Genome 2021 Jul 12:1-11. Epub 2021 Jul 12.

School of Life Sciences, Nantong University, Nantong, Jiangsu 226019, P.R. China.

Cotton ( L.) is the most important fiber crop worldwide. Here, transcriptome analysis was conducted on developing fibers of a introgression line, IL9, and its recurrent parent, PD94042, at 17 and 21 days post-anthesis (dpa). Differentially expressed genes (DEGs) of PD94042 and IL9 were identified. Gene Ontology (GO) enrichment analysis showed that the annotated DEGs were rich in two main biological processes and two main molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis likewise showed that the annotated DEGs were mainly enriched in metabolic pathways and biosynthesis of secondary metabolites. In total, 52 DEGs were selected as candidate genes based on comparison of the DEGs and GO function annotation information. Quantitative real-time PCR (RT-qPCR) analysis results for 12 randomly selected DEGs were consistent with transcriptome analysis. SNP identification based on chromatin segment introgression showed that 394 SNPs were identified in 268 DEGs, and two genes with known functions were identified within fiber strength quantitative trait loci (QTL) regions or near the confidence intervals. We identified 52 key genes potentially related to high fiber strength in a introgression line and provided significant insights into the study of cotton fiber quality improvement.
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http://dx.doi.org/10.1139/gen-2020-0177DOI Listing
July 2021

Enhancement effect of nanofluids on the desulfurization and regeneration performance of ionic liquid-based system.

J Hazard Mater 2021 Jun 11;419:126394. Epub 2021 Jun 11.

College of Resources and Environmental Engineering, Shandong University of Technology, Zibo 255049, PR China.

The desulfurization and regeneration performance of nanofluids composed of oxidizing ionic liquids and four inert nanoparticles are investigated. The addition of different nanoparticles has been proved to have enhancement effect on the HS removal performance of oxidizing ionic liquids. The nanofluids with SiO nanoparticles showed the most significant strengthening desulfurization performance as well as regeneration performance. The optimal weight ratio of SiO nanoparticles in nanofluids was confirmed as 0.5%. The regeneration efficiency of the optimal nanofluid system can exceed 88%, which is far higher than that before the addition of SiO nanoparticles. The mass transfer coefficient increased significantly after the addition of nanoparticles. The nanoparticles and nanofluids before and after absorption were characterized by Fourier transform infrared spectra, nuclear magnetic resonance, scanning electron microscope, transmission electron microscope, energy dispersive spectrum and X-ray photoelectron spectroscopy. It was found that the structure and morphology of SiO nanoparticles remained basically unchanged in the absorption-regeneration process. The main final desulfurization product was identified as sulfate.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126394DOI Listing
June 2021

MoSep3 and MoExo70 are needed for MoCK2 ring assembly essential for appressorium function in the rice blast fungus, Magnaporthe oryzae.

Mol Plant Pathol 2021 Jun 11. Epub 2021 Jun 11.

State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Polar growth during appressorium formation is vital for the penetration peg formation in the rice blast fungus, Magnaporthe oryzae. Previous research has shown that the Sln1-septin-exocyst complex, localized at the base of the appressorium in contact with the leaf surface, forms a ring structure that influences growth polarity and affects penetration peg formation, and is necessary for pathogenicity. Our previous research showed CK2 proteins assemble another ring structure positioned perpendicular to the Sln1-septin-exocyst complex. Our research showed that the CK2 ring needs to become correctly assembled for penetration peg function and subsequent plant infection. In the present study, we found that the ring structures of CK2 are absent in the appressorium of ΔMoSep3 septin deletion mutants lacking the septin ring of the Sln1-septin-exocyst complex. Sln1 affects the septin proteins that recruit the exocyst complex that localizes as another ring at the appressorium's bottom. Destruction of the exocyst complex by mutation also causes incorrect localization of the CK2 ring structure. In conclusion, CK2 probably takes part in reestablishing the appressorium' spolarity growth necessary for penetration peg formation. We can also conclude that the correct localization and assembly of one or more CK2 ring structures in the appressorium depend on the initial assembly of the Sln1-septin-exocyst complex two rings at the base of the appressorium.
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http://dx.doi.org/10.1111/mpp.13092DOI Listing
June 2021

The molecular mechanism underlying pathogenicity inhibition by sanguinarine in Magnaporthe oryzae.

Pest Manag Sci 2021 Jun 11. Epub 2021 Jun 11.

Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, China.

Background: Sanguinarine (SAN) is a benzophenanthridine alkaloid that broadly targets a range of pathways in mammalian and fungal cells. In this study we set out to explore the molecular mechanism of sanguinarine inhibition of the fungal development and pathogenicity of Magnaporthe oryzae with the hope that sanguinarine will bolster the development of antiblast agents.

Results: We found that the fungus exhibited a significant reduction in vegetative growth and hyphal melanization while the spores produced long germ tubes on the artificial hydrophobic surface characteristic of a defect in thigmotropic sensing when exposed to 4, 8 and 0.5 μm sanguinarine, respectively. Consistent with these findings, we observed that the genes involved in melanin biosynthesis and the fungal hydrophobin MoMPG1 were remarkably suppressed in mycelia treated with 8 μm sanguinarine. Additionally, sanguinarine inhibited appressorium formation at a dose of 1.0 μm and this defect was restored by supplementing 5 mM of exogenous cAMP. By qRT-PCR assay we found cAMP pathway signalling genes such as MoCAP1 and MoCpkA were significantly repressed whereas MoCDTF1 and MoSOM1 were upregulated in sanguinarine-treated strains. Furthermore, we showed that sanguinarine does not selectively inhibit vegetative growth and appressorium formation of Guy11 but also other strains of M. oryzae. Finally, treatment of sanguinarine impaired the appressorium-mediated penetration and pathogenicity of M. oryzae in a dose-dependent manner.

Conclusion: Based on our results we concluded that sanguinarine is an attractive antimicrobial candidate for fungicide development in the control of rice blast disease.
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http://dx.doi.org/10.1002/ps.6508DOI Listing
June 2021

Phenotype and management of chronic obstructive pulmonary disease patients in general population in China: a nationally cross-sectional study.

NPJ Prim Care Respir Med 2021 Jun 1;31(1):32. Epub 2021 Jun 1.

Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, China.

This study aims to investigate the characteristics of the phenotype and management of chronic obstructive pulmonary disease (COPD) patients in the general population in China. We analyzed spirometry-confirmed COPD patients who were identified from a population-based, nationally representative sample in China. All participants were measured with airflow limitation severity based on post-bronchodilator FEV percent predicted, bronchodilator responsiveness, exacerbation history, and respiratory symptoms. Among a total of 9134 COPD patients, 90.3% were non-exacerbators, 2.9% were frequent exacerbators without chronic bronchitis, 2.0% were frequent exacerbators with chronic bronchitis, and 4.8% were asthma-COPD overlap. Less than 5% of non-exacerbators ever had pulmonary function testing performed. The utilization rate of inhaled medication in non-exacerbators, exacerbators without chronic bronchitis, exacerbators with chronic bronchitis, and asthma-COPD overlap was 1.4, 23.5, 29.5, and 19.4%, respectively. A comprehensive strategy for the management of COPD patients based on phenotype in primary care is urgently needed.
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http://dx.doi.org/10.1038/s41533-021-00243-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169915PMC
June 2021

High-quality genome assembly of Pseudopestalotiopsis theae, the pathogenic fungus of tea grey blight.

Plant Dis 2021 May 12. Epub 2021 May 12.

Fujian Agriculture and Forestry University, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Plant Protection, Fuzhou, Fujian, China;

Tea grey blight is one of the most serious foliar diseases of tea tree caused by the plant pathogenic fungus Pseudopestalotiopsis theae which can affect production and quality of tea worldwide. We generated a highly contiguous, 50.41Mbp genome assembly (N50 1.30 Mbp) of P. theae strain CYF27 by combining PacBio long-read and Illumina short-read sequencing technologies. We identified a total of 15,626 gene models, of which 1,038 genes encode putative secreted proteins. The high-quality genome assembly and annotation resource reported here will be useful for the study of fungal infection mechanisms and pathogen-host interaction.
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http://dx.doi.org/10.1094/PDIS-02-21-0318-ADOI Listing
May 2021

LINC00943 knockdown exerts neuroprotective effects in Parkinson's disease through regulates CXCL12 expression by sponging miR-7-5p.

Genes Genomics 2021 Jul 22;43(7):797-805. Epub 2021 Apr 22.

Department of Neurology, Jingmen No. 1 People's Hospital, No. 168, Xiangshan Avenue, Duodao District, Jingmen, 448000, Hubei, China.

Background: Parkinson's disease (PD) is a common neurodegenerative movement disorder, but the pathogenesis is still unclear. Long non-coding RNAs (lncRNAs) have been reported to play a prominent role in PD.

Objective: This study is designed to explore the role and mechanism of long intergenic non-coding RNA 00943 (LINC00943) in the N-methyl-4-phenylpyridine (MPP)-inducted PD model.

Methods: LINC00943, microRNA-7-5p (miR-7-5p), and the chemokine (C-X-C motif) ligand 12 (CXCL12, also referred to as SDF-1) level were examined by real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability and apoptosis were analyzed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT), and flow cytometry assays, severally. Protein levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and CXCL12 were assessed by western blot assay. The ROS generation and SOD activity were detected by the corresponding kits. The binding relationship between miR-7-5p and LINC00943 or CXCL12 was predicted by Starbase and then verified by a dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays.

Results: LINC00943 and CXCL12 were increased, and miR-7-5p was decreased in MPP-inducted SK-N-SH cells. LINC00943 silencing promoted cell viability, and repressed apoptosis and the inflammatory response in MPP-treated SK-N-SH cells. The mechanical analysis discovered that LINC00943 acted as a sponge of miR-7-5p to regulate CXCL12 expression.

Conclusions: LINC00943 knockdown could attenuate MPP-triggered neuron injury by regulating the miR-7-5p/CXCL12 axis, hinting at a promising therapeutic target for PD treatment.
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http://dx.doi.org/10.1007/s13258-021-01084-1DOI Listing
July 2021

Focused Ultrasound-Augmented Cancer Phototheranostics Using Albumin-Indocyanine Green Nanoparticles.

Ultrasound Med Biol 2021 07 3;47(7):1801-1813. Epub 2021 Apr 3.

Department of Ultrasound Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China & Zhejiang Provincial Key Laboratory of Pulsed Electric Field Technology for Medical Transformation, Hangzhou, Zhejiang, China. Electronic address:

The purpose of this study was to develop a nanoparticle (NP) drug-loading system that enhances the efficiency of indocyanine green (ICG) entry into the tissue under focused ultrasound optimization and, in turn, enables more efficient identification and photothermal therapy (PTT) of the tumor. The new NPs were prepared by assembling intermolecular disulfide bonds to form human serum albumin (HSA) NPs and then conjugating those with ICG dye. The NP material was used to test the sensitivity of near-infrared fluorescence imaging and photoacoustic tumor imaging in vitro and in vivo. In addition, the combination of HSA-ICG NPs, focused ultrasound, and microbubbles was used to test PTT on the tumor. HSA-ICG NPs had good biocompatibility and were only a little toxic to cells and mice. In addition, they obviously enhanced tumor near-infrared fluorescence and photoacoustic bimodal imaging. Combined with HSA-ICG NPs, the depth of photoacoustic imaging was increased. Moreover, ICG that was absorbed in the HSA NPs promoted optical absorption in the near-infrared region, which greatly enhanced the PTT treatment's efficiency. This new bimodal tumor-imaging agent enhances the therapeutic effect of PTT and improves the detection of tumors in vivo, thus presenting great potential for use in clinical studies.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.02.026DOI Listing
July 2021

GRSF1 promotes tumorigenesis and EMT-mediated metastasis through PI3K/AKT pathway in gastric cancer.

Biochem Biophys Res Commun 2021 05 1;555:61-66. Epub 2021 Apr 1.

Department of General Surgery & Institute of General Surgery, Chinese PLA General Hospital, Beijing, China. Electronic address:

It is vital to identify effective therapeutic targets and explore the underlying mechanisms to curb the progression of Gastric cancer (GC) and improve the prognosis of GC patients. Guanine-rich RNA sequence binding factor 1 (GRSF1) is a member of the RNA-binding protein family. The present study showed that GRSF1 knockdown suppressed GC cells proliferation, migration and invasion in vitro, while GRSF1 overexpression enhanced the proliferation, migration and invasion of GC cells. Meanwhile, knockdown of GRSF1 inhibited tumor growth and tumor metastasis in vivo. Furthermore, we demonstrated that GRSF1 induced epithelial-mesenchymal transition (EMT) and activated PI3K/AKT pathway in vitro and in vivo through gain and loss of function. In conclusion, we demonstrated that GRSF1 promotes tumorigenesis and EMT-mediated metastasis through PI3K/AKT pathway in GC. Our study for the first time identified the functions of GRSF1 serving as an oncogene in GC, which may be a potential effective therapeutic target and malignant indicator in GC.
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http://dx.doi.org/10.1016/j.bbrc.2021.03.121DOI Listing
May 2021

Study on the desulfurization performance of iron/ethanolamine/deep eutectic solvent system.

Environ Sci Pollut Res Int 2021 Jul 16;28(28):38026-38033. Epub 2021 Mar 16.

College of Resources and Environmental Engineering, Shandong University of Technology, Zibo, 255049, People's Republic of China.

Deep eutectic solvent (DES) was applied as the solvent of iron/alcohol amine system, and the prepared iron/ethanolamine/DES system was found to be a good desulfurizer for HS removal. The absorbents were characterized by Fourier transform infrared spectroscopy. The iron/ethanolamine/DES system showed a significantly enhanced desulfurization performance compared with DES solution of iron or alcohol amine separately. Besides, the absorbents showed relatively stable desulfurization performance, which could keep a high HS removal efficiency in a wide temperature range from 30-90°C. The iron/ethanolamine/DES system could be recycled for at least three times. The desulfurization product was analyzed by energy dispersive spectrum and X-ray diffraction, and the desulfurization product was identified as sulfur element.
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http://dx.doi.org/10.1007/s11356-021-13487-4DOI Listing
July 2021

Shear deformation response of a holographic sensor based on elastic poly(MMA-co-LMA) photopolymer.

Opt Lett 2021 Mar;46(6):1249-1252

A holographic sensor based on camphorquinon doped poly (methyl methacrylate-co-lauryl methacrylate) (poly (MMA-co-LMA)) elastic photopolymer is developed for characterizing the shear deformation of material. A shear angle and its transverse displacement, which are induced by a couple of shear stresses, are analyzed using a diffraction spectrum of a transmission holographic sensor. The dependence of the peak wavelength shift on the shear deformation presents a good linear relationship which provides a quantitative characterization means. The detectable maximum of the shear angle exceeds 26.1 deg, and the peak wavelength shift closes to 4.0 nm. The available sensitivity is better than 3.33 deg/0.5 nm (shear angle/wavelength shift) using a commercial spectrometer with 0.5 nm of resolution. Finally, the reversibility response of shear deformation further confirmed the practical applicability of the elastic polymer-based shear deformation sensor. The spectrum measurement of shear deformation provides a novel measurement means for the mechanical deformation of materials and expands the application of a holographic sensor.
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http://dx.doi.org/10.1364/OL.413162DOI Listing
March 2021

Sanhuang Jiangtang tablet protects type 2 diabetes osteoporosis via AKT-GSK3β-NFATc1 signaling pathway by integrating bioinformatics analysis and experimental validation.

J Ethnopharmacol 2021 Jun 26;273:113946. Epub 2021 Feb 26.

Department of Orthopaedics, First Affiliated Hospital, Guangzhou University of Chinese Medicine, 12 Jichang Road, Baiyun Area, Guangzho, 510405, PR China. Electronic address:

Ethnopharmacological Relevance: Sanhuang Jiangtang tablet (SHJTT), has been widely used to treat type 2 diabetes mellitus (T2DM). However, the potential and mechanism of SHJTT in treating type 2 diabetes osteoporosis (T2DOP) has not been reported.

Aim Of The Study: The aim of this work was to investigate the role and the underlying molecular mechanism of SHJTT in managing type 2 diabetes osteoporosis.

Materials And Methods: The target genes of each component consisting of SHJTT were obtained by searching the ETCM database. The target genes of osteoporosis and diabetes were individually acquired by analyzing the DisGeNET and OMIM disease databases. Then the potential therapeutic genes were obtained from the intersection of the herbal medicine targets and the disease targets which were imported into the R and STRING platform for the analysis of GO terms, KEGG pathways and PPI network. The key modules of PPI network were constructed by Cytoscape software. Finally, leptin receptor deficiency (db/db) mice were confirmed as an animal model of type 2 diabetic osteoporosis (T2DOP) through phenotype assessment and the key genes of SHJTT against T2DOP were validated by quantitative real-time PCR (qRT-PCR).

Results: A total of 786 target genes of SHJTT were obtained from ETCM. Simultaneously, a total of 3906 osteoporosis and type 2 diabetes associated targets were acquired from DisGeNET and OMIM databases. Then, 97 common targets were found by overlapping them. On the basis of the GO and KEGG enrichment analysis and PPI network, we found that the related pathway of SHJTT in type 2 diabetes osteoporosis was AKT-GSK3β-NFATc1 pathway which is tightly associated with osteoclast differentiation. The expression of key genes including Akt1, Mapk3, Gsk3β, Mmp9, Nfkb1 were significantly down-regulated by SHJTT in T2DOP mice (p < 0.05).

Conclusions: SHJTT had a protective effect on T2DOP via regulating AKT-GSK3β-NFATc1 signaling pathway. This study might provide a theoretical basis for the application of SHJTT for the treatment of type 2 diabetic osteoporosis.
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http://dx.doi.org/10.1016/j.jep.2021.113946DOI Listing
June 2021

A Method of Mining Truck Loading Volume Detection Based on Deep Learning and Image Recognition.

Sensors (Basel) 2021 Jan 18;21(2). Epub 2021 Jan 18.

School of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China.

Detection of the loading volume of mining trucks is an important task in open pit mining. Aiming at the addressing the current problems of low accuracy and high cost of the detection of the loading volume of mining trucks, this paper proposes a mining truck loading volume detection model based on deep learning and image recognition. The training and test data of the model consists of 6000 sets of images taken in a laboratory environment. After image preprocessing, the VGG16 network model is used to pre classify the ore images. The classification results are displayed and the possibility of each category is determined. Then, the loading volume of mining trucks is calculated by using the classification results and the least squares algorithm. By using the labeled image data of five kinds of mining truck loading volume, the arbitrary loading volume detection of mining trucks is realized, which effectively solves the problem of a lack of labeled data types caused by the difficulty in obtaining mine data. Root mean square error (RMSE) and mean absolute error (MAE) are used to evaluate the fitting effect of the model. The experimental results show that the model has high prediction accuracy. The average absolute error is 17.85 cm3. In addition, this paper uses 400 real mining truck images of open-pit mines to verify the model and the average absolute error is 2.53 m3. The experimental results show that the model has good generality and can be applied well to the actual production of open-pit mines.
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http://dx.doi.org/10.3390/s21020635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831092PMC
January 2021

Lung cancer chemotherapy using nanoparticles: Enhanced target ability of redox-responsive and pH-sensitive cisplatin prodrug and paclitaxel.

Biomed Pharmacother 2021 Apr 12;136:111249. Epub 2021 Jan 12.

Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Tumor Hospital of Hebei Province, Shijiazhuang, 050011, Hebei Province, People's Republic of China. Electronic address:

Platinum-based combination therapy is more effective and less toxic, but lack of targeting, and is not capable to enrich in the tumor zone. To obstacle these drawbacks, prodrug and nanotechnology strategies have been investigated in this study. GSH-responsive and pH-responsive cisplatin prodrug was synthesized. Cisplatin prodrug and paclitaxel co-loaded nanoparticles: DDP-P/PTX NPs were constructed. The drug release behavior and cytotoxicity of nanoparticles was assessed in vitro. In vivo anticancer efficiency and toxicity were evaluated on lung cancer bearing mice animal model. DDP-P/PTX NPs had a nanoscale size of 112.9 ± 3.5 nm. A reduction and pH triggered drug release with a synergistic tumor cell inhibition ability was observed by DDP-P/PTX NPs. DDP-P/PTX NPs also exhibited high tumor distribution, low systemic toxicity and remarkable antitumor effects in vivo. DDP-P/PTX NPs could be applied as promising anticancer system for the treatment of NSCLC.
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http://dx.doi.org/10.1016/j.biopha.2021.111249DOI Listing
April 2021

Spatiotemporal variations in ischemic heart disease mortality and related risk factors in China between 2010 and 2015: a multilevel analysis.

BMC Public Health 2021 01 4;21(1). Epub 2021 Jan 4.

National Center for Chronic and Noncommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Xicheng District, Beijing, 100050, P.R. China.

Background: To explore the relationship between geographical differences of mortality and related risk factors in ischemic heart disease (IHD) in China.

Methods: Data were collected from the nationally representative China Mortality Surveillance System to calculate annual IHD mortality counts (2010-2015). Descriptive analysis was used to analyze the IHD mortality among Chinese population from 2010 to 2015. Negative binomial regression was used to investigate potential spatiotemporal variation and correlations with age, gender, urbanization, and region.

Results: The overall IHD mortality was 221.17/100,000, accounting for 1.51 million deaths in 2015. The standardized IHD mortality rate increased by 5.51% from 2010 to 2015 among people aged 40 years and older. Multilevel analysis indicated significant differences in gender, regions, and age. High urbanization rate (risk ratio [RR] = 0.728, 95% confidence interval [CI] = (0.631, 0.840)) and average high-density lipoprotein (HDL) (RR = 0.741, 95%CI: 0.616,0.891) were negatively associated with IHD mortality. IHD mortality was significantly higher in populations with a low rate of medical insurance coverage (RR = 1.218, 95%CI: 1.007, 1.473), as well as the average body mass index (BMI) (RR = 1.436, 95%CI: 1.135, 1.817) and systolic blood pressure (SBP) (RR = 1.310, 95%CI: 1.019, 1.684). While the relationship with current smoking rate, excessive intake of red meat, insufficient vegetable or fruits intake didn't show the statistical significance. The negative correlation between the average sedentary time and IHD mortality was not conclusive due to the possible deviation of the data.

Conclusions: The mortality of IHD showed an upward trend for people aged 40 years and older in China during 2010-2015, which should be paid attention to. Therefore, some risk factors should be controlled, such as SBP, overweight/obesity. HDL is a protective factor, as well as higher urbanization rate, family income level, and medical insurance coverage.
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http://dx.doi.org/10.1186/s12889-020-10019-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784031PMC
January 2021

Metastasis of Ovarian Cancer to Breast: A Case Report and Review of Imaging Manifestations.

Cancer Manag Res 2020 18;12:13015-13021. Epub 2020 Dec 18.

Department of Ultrasound, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, People's Republic of China.

Metastasis of ovarian cancer to breast (MOCB) is rare, and the diagnosis is difficult due to the varied imaging manifestations. The objective of this paper is to report a special case of MOCB, review the imaging manifestations of MOCB and attempt to determine the characteristic imaging features that might be helpful in making the diagnosis and providing appropriate systemic therapy. A 40-year-old woman presented with a breast lesion six years after a diagnosis of ovarian serous cystadenocarcinoma. Ultrasound (US) and magnetic resonance imaging (MRI) examinations were performed; the final diagnosis was metastasis of ovarian serous cystadenocarcinoma to breast according to the histological examination and immunohistochemical examination after lumpectomy. Herein, we reviewed 41 cases diagnosed with MOCB, which include imaging of the metastatic breast lesion or a detailed description without imaging. In this review, we summarized that MOCB could present with calcifications (especially microcalcifications) on mammography (MM). MOCB presenting as inflammatory breast cancer usually shows swelling and skin thickening on MM and US, and increased fludeoxyglucose (FDG) uptake on positron emission tomography/computed tomography (PET/CT). These manifestations could be helpful in differentiating malignant tumors from benign ones, but it may still be difficult to distinguish between primary and metastatic tumors. The correct diagnosis of MOCB requires a combination of the clinical history of the primary tumor, careful clinical examination, radiology and anatomic pathological evaluation.
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http://dx.doi.org/10.2147/CMAR.S280795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755876PMC
December 2020

Enhanced removal of hydrogen sulfide using novel nanofluid system composed of deep eutectic solvent and Cu nanoparticles.

J Hazard Mater 2021 03 13;405:124271. Epub 2020 Oct 13.

College of Resources and Environmental Engineering, Shandong University of Technology, Zibo 255049, PR China.

The HS removal performances of four deep eutectic solvent (DES) based nanofluid (NF) systems were measured using dynamic absorption experiment. The Cu containing NF system is found to be an excellent absorbent for HS removal with a significantly enhanced desulfurization performance compared with DES original solution. Besides, the NF systems have relatively high regeneration performance. The NF systems and Cu nanoparticles before and after absorption as well as after regeneration were characterized by Fourier transform infrared (FT-IR) spectra, scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), transmission electron microscope (TEM) and energy dispersive spectrum (EDS). It is found that the ethanolamine, choline cation and sulfur were accumulated on the surface of Cu nanoparticles after absorption, and the bulk elements on the surface were identified as Cu and S after regeneration. The S was existed in the form of CuS, and some sulfur was oxidized to zero-valent sulfur after regeneration.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124271DOI Listing
March 2021

Semi-embedded valve anastomosis a new anti-reflux anastomotic method after proximal gastrectomy for adenocarcinoma of the oesophagogastric junction.

BMC Surg 2020 Oct 8;20(1):230. Epub 2020 Oct 8.

Gastrointestinal Surgery, The second hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, China.

Background: There is a high probability of gastroesophageal reflux after laparoscopic proximal gastrectomy for adenocarcinoma of the oesophagogastric junction (AEG). Various anti-reflux anastomotic methods are emerging in clinical practice; however, none of them have been widely accepted. We have innovated a new type of anti-reflux anastomotic method, named semi-embedded valve anastomosis. The aim of this study was to explore the feasibility and anti-reflux effect of the new anastomotic method.

Methods: The clinical data of 28 patients with Siewert II AEG who were treated by semi-embedded valve anastomosis were collected. The key point of the operation is to reconstruct a simulated valve and form an anti-reflux barrier similar to the physiological mechanism. The gastroesophageal reflux disease questionnaire (GerdQ) and classification of gastroesophageal reflux under electron microscopy were used to evaluate gastroesophageal reflux after the operation.

Results: The mean operative duration was 164.3 ± 19.0 min, the median intraoperative haemorrhage volume was 65 ml, the average number of lymph nodes dissected was 23 ± 2.6, the time for valve construction was 15.8 ± 3.2 min, the time for anastomotic reconstruction was 35.4 ± 4.8 min, the median time to first flatus was 3 d, and the median hospitalization duration was 12 d. There was one case of postoperative anastomotic stenosis. The GerdQ score [median (range)] was as follows: 2 (0-6), preoperation; 0 (0-8), 1 month postoperation; 2 (0-12), 3 months postoperation; and 3 (0-12), 6 months postoperation. The Wilcoxon signed-rank sum test was carried out at different times after the operation and the day before the operation, and the differences were not significant. There was one case of grade B gastroesophageal reflux according to the Los Angeles classification system among the gastrofibroscopic re-examination reports of 28 cases.

Conclusion: Semi-embedded valve anastomosis is safe and feasible after proximal gastrectomy for Siewert II AEG and has good anti-reflux effects.
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http://dx.doi.org/10.1186/s12893-020-00894-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545939PMC
October 2020

Survival analysis of patients with primary gallbladder cancer from 2010 to 2015: A retrospective study based on SEER data.

Medicine (Baltimore) 2020 Oct;99(40):e22292

National Center for Chronic and Noncommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing.

This study aims to assess the survival status of patients with Primary gallbladder cancer (PGC) and analyze the prognosis factors to facilitate the exploration of the prevention and therapeutic strategies of PGC.Data from 2433 PGC patients collected from 2010 to 2015 were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. The SEER*Stat, SPSS 23.0 and GraphPad Prism 8 were used for statistical analyses. Kaplan Meier analysis was performed for the survival curve, log-rank test analyses were used to compare the survival rate difference and Cox regression analyses were performed to determine the prognosis factors.A total of 2433 PGC cases were reported from 2010 to 2015. The median age was 64.2 ± 10.4 years old and the percentages of the white patients were 73.7% (1794/2433). The percentage of patients who received surgery treatment was 82.1% (1998/2433). The overall median survival time of all patients was 19 months and the 5-year survival rate was 28.8%. The 5-year survival rate of PGC patients in pN2 stage dropped to 0% and the 5-year survival rate for PGC patients with distant metastasis was only 2.7%. Age, tumor size, grade, pT stage, pM stage were risk factors for prognosis, surgery or not and radiation or not were protective factors for prognosis.Survival analysis of PGC patients based on the SEER database have provided an opportunity for understanding PGC prognosis and the basis for the exploration of viable PGC prevention and therapeutic strategies.
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http://dx.doi.org/10.1097/MD.0000000000022292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7535694PMC
October 2020

Expansion of axial dispersion in a photopolymer-based holographic lens and its improvement for measuring displacement.

Appl Opt 2020 Sep;59(27):8279-8284

Coaxial multiple holographic lenses as high-dispersion elements are developed for a spectral confocal displacement measurement device. Wavelength and coaxial spatial multiplexing methods are used to record the holographic lens with two coaxial foci. The expansion of axial spatial dispersion in photopolymer-based multiple holographic lenses has been demonstrated and studied experimentally. The multiple holographic lenses provide a larger spatial dispersion to improve the characteristic parameters for measuring the displacement. Compared to single holographic lenses, the maximum of axial dispersion wavelength difference of the multiple lenses increases from 134.63 to 162.81 nm, and the corresponding measurable range increases from 203 to 385 mm. The axial spatial dispersion conforms to a typical exponential function. The overall spatial position sensitivity of multiple holographic lenses reaches 2.36 mm/nm. In addition, the multiple lenses also decrease the lateral dispersion compared to the single lenses. The multiple lenses can efficiently reduce the transverse measurement error. Finally, the displacement measurement result confirms the improvement of measureable spatial range. The multiple holographic lenses can accelerate the practical application of holographic lens-based optical elements.
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http://dx.doi.org/10.1364/AO.401431DOI Listing
September 2020

Long-Read Genome Sequence Resource of Causing Leaf Spot Disease in .

Mol Plant Microbe Interact 2020 Dec 26;33(12):1438-1440. Epub 2020 Oct 26.

Fujian University Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

is the fungal pathogen that causes the severe leaf spot disease of (Miq.) Pax, a vital Chinese herbal plant. Here, we deployed PacBio single-molecule real-time long-read sequencing technology to generate a near-complete genome assembly for the KC1 strain and obtained a total of 9.80 Gb raw reads. These reads were processed into a 41.05 Mb genome assembly containing 95 contigs with N50 of 1.70 Mb and a maximum length of 3.93 Mb. A total of 10,457 gene models, of which 1,004 encode putatively secreted proteins, were identified in the genome. This high-quality genome assembly and gene annotation resource will facilitate the institution of functional genetic studies aimed at providing a better insight into the infection mechanisms of to support the development of effective control strategies for leaf spot disease of
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http://dx.doi.org/10.1094/MPMI-05-20-0137-ADOI Listing
December 2020

Physical Activity, Step Counts, and Grip Strength in the Chinese Children and Families Cohort Study.

Int J Environ Res Public Health 2020 08 26;17(17). Epub 2020 Aug 26.

Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20850, USA.

Objectives: This paper describes the development of a physical activity questionnaire (PAQ) designed for Chinese adolescents and their mothers in urban and rural settings, and reports on results of the PAQ, pedometry, and hand grip dynamometry from the Chinese Children and Families Cohort Study pilot investigation (CFCS).

Methods: As part of a pilot investigation to evaluate the feasibility to follow-up and obtain detailed nutrition, dietary, physical activity, and ultraviolet radiation (UVR) data from CFCS participants, data were collected in 2013 for 93 adolescent/mother pairs from a rural ( = 41) and an urban site ( = 52) in two provinces. Respondents were asked to wear a pedometer for seven days (Omron HJ-151), use a Takei Digital Grip Strength Dynamometer on (each hand; three trials; two separate days), and complete a 39 item, eight domain PAQ covering the past year. Self-reported physical activity (PA) was linked to metabolic equivalent of task (MET) scores in kcal/kg/hr and used to calculate METs for different domains of PA and intensity categories.

Results: Compliance was high (95%) in this measurement protocol administered by health staff during a series of data collection efforts at home and local clinics or health centers. Step counts were highly variable, averaging between 5000 and 10000 per day with somewhat higher step counts in rural adolescent boys. Maximum grip strength (Kgs) was greater in children (Mean = 36.5, SE = 0.8) than mothers (Mean = 28.8, SE = 0.8) and similar in the urban (Mean = 29.6, SE = 0.6) compared to the rural (Mean = 29.6, SE = 0.5) communities overall. Grip strength, step counts, and measures of time spent in different activities or activity intensities were uncorrelated.

Conclusion: Device and question-based measurement of PA and strength were readily accepted in these Chinese urban and rural populations. The PAQ on physical activity in the past year produced some plausible population averages, but individual responses suggested recall challenges. If data about specific activities are required, future studies should explore use of standardized survey questions concerning such fewer specific activities or instruments examining shorter time periods such as one, three, or seven day recalls.
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http://dx.doi.org/10.3390/ijerph17176202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504127PMC
August 2020

Identification of potential bioactive compounds and mechanisms of GegenQinlian decoction on improving insulin resistance in adipose, liver, and muscle tissue by integrating system pharmacology and bioinformatics analysis.

J Ethnopharmacol 2021 Jan 23;264:113289. Epub 2020 Aug 23.

The First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China; Department of Endocrinology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address:

Ethnopharmacological Relevance: GegenQinlian Decoction (GQD), a classical formula in traditional Chinese medicine, is widely used in the treatment of diabetes. While studies have demonstrated that GQD is an efficacious treatment for insulin resistance (IR) in type 2 diabetes mellitus (T2DM), the potential bioactive compounds and mechanisms remain unclear.

Aim Of The Study: To further investigate the potential bioactive compounds, targets, and pathways of GQD on improving IR in T2DM for adipose, liver, and muscle tissue using an integrated strategy of system pharmacology and bioinformatics analysis.

Materials And Methods: We screened the candidate compounds and targets of GQD and identified IR-associated differentially expressed genes (DEGs) of adipose, liver, and muscle tissue, respectively. Then the intersecting target genes between candidate targets and DEGs were used for "GQD-compounds-targets-tissue" network construction in each type of tissue. The top 10 bioactive compounds acting on each type of tissue were intersected and consequently identified as potential bioactive compounds of GQD. Furthermore, pathway enrichment, protein-protein interaction (PPI) network construction, and hub target identification were performed based on the targets of GQD and the targets of quercetin in each type of tissue, respectively. Finally, to further confirm the role of quercetin, we intersected the hub targets of quercetin and the hub targets of GQD, and the pathways were intersected as well.

Results: 132 compounds and 119 potential targets of these compounds were obtained. 1,765, 3,206, and 3594 DEGs were identified between IR and insulin sensitivity (IS) tissue in adipose, liver, and muscle, respectively. There were 21, 23, 45 targets and 103, 73, 123 compounds in the "GQD-compounds-targets-tissue" network of adipose, liver, and muscle tissue, respectively. Then compounds such as quercetin, kaempferol, baicalein, wogonin, isorhamnetin, beta-sitosterol and licochalcone A, were identified as the potential bioactive compounds of GQD, and quercetin had the highest degree among the compounds. Moreover, based on the targets of GQD, hub targets like PPARG, RELA, EGFR, CASP3, VEGFA, AR, ESR1 and CCND1, and signaling pathways such as insulin signaling pathway, endocrine resistance, TNF signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, MAPK signaling pathway, NF-κB signaling pathway, HIF-1 signaling pathway, apoptosis, and VEGF signaling pathway, were filtered out as the underlying mechanisms of GQD on improving diabetic IR. In addition, the hub targets and pathways of quercetin coincided with most of the hub targets and pathways of GQD in each type of tissue, respectively, suggesting that quercetin may be a potential representative compound of GQD.

Conclusions: Our analysis identifies the potential bioactive compounds, targets, and pathways of GQD on improving IR in T2DM for adipose, liver, and muscle tissue, which shows the characteristics of multi-compounds, multi-targets, multi-pathways, and multi-mechanisms of GQD and lays a solid foundation for further experimental research and clinical application.
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http://dx.doi.org/10.1016/j.jep.2020.113289DOI Listing
January 2021

Neighborhood greenness associated with chronic obstructive pulmonary disease: A nationwide cross-sectional study in China.

Environ Int 2020 11 19;144:106042. Epub 2020 Aug 19.

National Center for Chronic and Non-communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address:

Differential findings have been reported on the association between neighborhood greenness and chronic obstructive pulmonary disease (COPD). The underlying reasons might be the different types of vegetation and the diagnosis methods used in different studies. In this nationwide cross-sectional study in China, we examined the linkage between neighborhood greenness and COPD prevalence among 66,752 adults aged 40 years and above. Neighborhood greenness was estimated using the normalized difference vegetation index (NDVI) based on satellite imagery within buffers of 100, 300, 500, 1000, 2000, 3000 and 5000 m of residential community of the participants. COPD was defined according to the 2017 Global Initiative for Chronic Obstructive Lung Disease lung function criteria. A two-level logistic regression model was applied to estimate the associations. Finally, 9134 adults were classified as COPD. We observed significant positive associations between neighborhood greenness and COPD prevalence. The odds ratio for each interquartile range increase in NDVI within 100 m buffer was 1.08 (95% CI: 1.01, 1.15) after adjustment for potential confounders. Consistent associations were observed across all other NDVI buffer sizes. Stratified analyses revealed that younger adults (40-65 years) and urban residents might be the vulnerable subpopulations. Further regional analyses found that residents from the Northeastern and Northern China were more likely to have this association. Our results indicated that neighborhood greenness might be one risk factor of COPD prevalence. Our study have important public health implications for allocating the surrounding green spaces among living areas, especially for those with respiratory illness; however, the findings and the underlying mechanisms warrant further examinations in longitudinal settings.
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http://dx.doi.org/10.1016/j.envint.2020.106042DOI Listing
November 2020

Associations Between Salt-Restriction Spoons and Long-Term Changes in Urinary Na/K Ratios and Blood Pressure: Findings From a Population-Based Cohort.

J Am Heart Assoc 2020 07 17;9(14):e014897. Epub 2020 Jul 17.

Shandong Center for Disease Control and Prevention Jinan China.

Background There have been few studies on the relationship between long-term changes in sodium intake and blood pressure. A method of reducing sodium intake in a population that is known for high-sodium intake based on homemade cooking is also needed. Methods and Results Our study was based on a baseline survey of 15 350 individuals aged 18 to 69 years with multistage random sampling and a 3-year salt-restriction campaign across Shandong Province, China. We included 339 individuals from six districts/counties in this cohort study, and the 24-hour urinary sodium-potassium ratio (Na/K) served as an indicator of sodium intake. The average change in ratio was 2.39 (95% CI, 2.17-2.61) from 6.81 (95% CI, 6.41-7.21) at baseline to 4.41 (95% CI, 4.18-4.64) during the resurvey. Following a reduction from low to high quartiles of urinary Na/K ratio, the average increases were 10.9 (95% CI, 8.9-12.9), 9.2 (95% CI, 6.9-11.5), 6.3 (95% CI, 4.0-8.6), and 5.3 (95% CI, 2.9-7.7) mm Hg for systolic blood pressure ( for trend=0.019) and 3.8 (95% CI, 2.4-5.2), 2.9 (95% CI, 1.7-4.2), 1.6 (95% CI, 0.4-2.8), and -0.3 (95% CI, -1.4-0.8) mm Hg for diastolic blood pressure ( for trend=0.002), respectively. A reduction in salt intake was evident for people using a 2-g salt-restriction spoon for cooking (-3.49 versus -2.22; =0.027) after adjustment of confounding factors, compared with nonusers. Similar findings were obtained for other salt-restriction spoon-based indicators. Conclusions Our study indicated that using a salt-restriction spoon for cooking was associated with reduced salt intake that led to a blunting of blood pressure deterioration. This finding further supports the salt-restriction spoon-based strategy for people whose primary salt intake is from homemade cooking.
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http://dx.doi.org/10.1161/JAHA.119.014897DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660739PMC
July 2020

Study on the Desulfurization and Regeneration Performance of Functional Deep Eutectic Solvents.

ACS Omega 2020 Jun 17;5(25):15353-15361. Epub 2020 Jun 17.

College of Resources and Environmental Engineering, Shandong University of Technology, Zibo 255049, P. R. China.

Four deep eutectic solvents (DESs) were synthesized, and 5-30% polyethylenimine (PEI) was added to make functional DESs (FDESs) for dynamic absorption experiments of hydrogen sulfide. The synthesized FDESs were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and nuclear magnetic resonance. The results demonstrated the successful synthesis of FDESs. The interaction between HS and the FDESs was discussed at a molecular level via the quantum chemical calculations. It was noticed that FDESs prefer chemisorption on HS. In this work, the 25% PEI/[email protected] showed the highest desulfurization performance. The effects of HS concentration and temperature on the desulfurization performance were investigated. It was found that a relatively low temperature (30 °C) was favorable for the absorption of HS. The 25% PEI/[email protected] could remove HS efficiently over a low HS concentration. Moisture played an important role in the FDES desulfurization system. The absorption/desorption cycle experiment indicated that the FDESs retain their good regeneration performance for at least five times.
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http://dx.doi.org/10.1021/acsomega.0c01467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331076PMC
June 2020

Combination Chemotherapy of Lung Cancer - Co-Delivery of Docetaxel Prodrug and Cisplatin Using Aptamer-Decorated Lipid-Polymer Hybrid Nanoparticles.

Drug Des Devel Ther 2020 9;14:2249-2261. Epub 2020 Jun 9.

Department of Thoracic Surgery, Fourth Hospital of Hebei Medical University, Tumor Hospital of Hebei Province, Shijiazhuang, Hebei Province, People's Republic of China.

Purpose: Lung cancer is the leading cause of cancer mortality worldwide. Drug resistance is the major barrier for the treatment of non-small cell lung cancer (NSCLC). The aim of this research is to develop an aptamer-decorated hybrid nanoparticle for the co-delivery of docetaxel prodrug (DTXp) and cisplatin (DDP) and to treat lung cancer.

Materials And Methods: Aptamer-conjugated lipid-polymer ligands and redox-sensitive docetaxel prodrug were synthesized. DTXp and DDP were loaded into the lipid-polymer hybrid nanoparticles (LPHNs). The targeted efficiency of aptamer-decorated, DTXp and DDP co-encapsulated LPHNs (APT-DTXp/DDP-LPHNs) was determined by performing a cell uptake assay by flow cytometry-based analysis. In vivo biodistribution and anticancer efficiency of APT-DTXp/DDP-LPHNs were evaluated on NSCLC-bearing mice xenograft.

Results: APT-DTXp/DDP-LPHNs had a particle size of 213.5 ± 5.3 nm, with a zeta potential of 15.9 ± 1.9 mV. APT-DTXp/DDP-LPHNs exhibited a significantly enhanced cytotoxicity (drug concentration causing 50% inhibition was 0.71 ± 0.09 μg/mL), synergy antitumor effect (combination index was 0.62), and profound tumor inhibition ability (tumor inhibition ratio of 81.4%) compared with the non-aptamer-decorated LPHNs and single drug-loaded LPHNs.

Conclusion: Since the synergistic effect of the drugs was found in this system, it would have great potential to inhibit lung tumor cells and in vivo tumor growth.
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http://dx.doi.org/10.2147/DDDT.S246574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7293388PMC
March 2021

Genome Sequence of , the Causal Agent of Olive Leaf Scab.

Mol Plant Microbe Interact 2020 Sep 2;33(9):1095-1097. Epub 2020 Jul 2.

Fujian University Key Laboratory for Plant-Microbe Interaction, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Olive leaf scab, also known as peacock spot disease, caused by (syn. and ) is the most widespread and economically important fungal disease attacking olive in production countries. Here, we report the first highly contiguous whole-genome sequence (46.08 Mb) of one isolate, YUN35, of The described genome sequence and annotation resource will be useful to study the fungal biology, pathogen-host interaction, characterization of genes of interest, and population genetic diversity.
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http://dx.doi.org/10.1094/MPMI-03-20-0070-ADOI Listing
September 2020
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