Publications by authors named "Baohua Li"

210 Publications

Protocol for the National Nurse Health Study (NNHS): a web-based ambispective cohort study.

BMJ Open 2021 08 19;11(8):e049958. Epub 2021 Aug 19.

Research Center of Clinical Epidemiology, Peking University Third Hospital, Beijing, China

Introduction: The physical and mental health of nurses may significantly impact the entire medical care group and directly affect the quality of medical services. Due to the intense emotional involvement and often problematic working conditions that characterise their profession, nurses appear to be especially susceptible to a complex set of stressors with repercussions to their health. Several landmark studies of nurses have provided an abundance of evidence on risk factors that influence the health status of nurses. However, few studies have investigated the health status of nurses who work in high-intensity work environments in China. The National Nurse Health Study (NNHS) objective is to build an ambispective cohort to gather web-based information on early-life events, daily habits, occupational and environmental risk factors, and health outcomes of a specific subset of healthcare professionals of Chinese nurses.

Methods And Analysis: NNHS, which was developed at a tertiary hospital in Beijing, China, is a research initiative that enrolls registered nurses working at Peking University Third Hospital. A web-based self-administered system was designed to collect health-related data and link them with previous physical examination data. During the study period, participants with signed informed consent will be invited to annually repeat a similar procedure.

Ethics And Dissemination: The NNHS research protocol was approved by the Institutional Ethics Committee and provides promising data that contribute to the understanding of pathophysiological links between early-life events, body composition, gut microbiota, and inflammatory and metabolic risk profiles. Moreover, the combination of a user-friendly tool with the innovative purposes of the NNHS offers a remarkable resource to test hypotheses about mechanisms of diseases, including work stress, and further plan preventive programmes in public health.

Trial Registration Number: The study was registered on Clinicaltrials.gov (https://clinicaltrials.gov/ct2/show/NCT04572347) and the China Cohort Consortium (http://chinacohort.bjmu.edu.cn/project/102/).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjopen-2021-049958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378396PMC
August 2021

β-Glucosidase Contributes to the Virulence of in Apple Tree.

Front Microbiol 2021 30;12:695112. Epub 2021 Jul 30.

Key Laboratory of Integrated Crop Pest Management of Shandong Province, Shandong Province Key Laboratory of Applied Mycology, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.

The apple tree canker is caused by , which produces major pathogenic factors involving multiple cell wall-degrading enzymes (CWDEs) and toxins. The β-glucosidases are among the main CWDEs, and thus, they play important roles in the virulence of necrotrophic pathogens. However, the specific roles of β-glucosidases in the virulence of remain largely unknown. In this study, we identified a β-glucosidase gene, V, which was upregulated during the infection. We found that VmGlu2 protein had high enzyme activity of β-glucosidase using p-nitrophenyl-β-D-glucopyranoside (pNPG) as a substrate, while the VmGlu2 could convert phloridzin to phloretin with the release of glucose. The deletion and overexpression of showed no effect on vegetative growth, but gene deletion mutants of showed significantly reduced pycnidia formation. The gene deletion mutants had lower β-glucosidase activities and toxin levels as compared to the wild-type strain. Therefore, these mutants showed a reduced virulence. Moreover, the overexpression of did not affect toxin levels, but it significantly enhanced β-glucosidase activities, which resulted in an increased pathogenicity. Thus, we conclude that is required for the full virulence of . These results provide valuable evidence to the complex role of CWDEs in the fungal pathogenicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.695112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361449PMC
July 2021

Hydroxybenzoate hydroxylase genes underlying protocatechuic acid production in Valsa mali are required for full pathogenicity in apple trees.

Mol Plant Pathol 2021 Aug 13. Epub 2021 Aug 13.

College of Plant Health and Medicine, Key Laboratory of Integrated Crop Pest Management of Shandong Province, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, China.

Valsa mali is the causative agent of apple tree valsa canker, which causes significant losses in apple production. It produces various toxic compounds that kill plant cells, facilitating infection. Among these, protocatechuic acid exhibits the highest phytotoxic activity. However, those genes involved in toxin production have not been studied. In this study we identified four hydroxybenzoate hydroxylase genes (VmHbh1, VmHbh2, VmHbh3, and VmHbh4) from the transcriptome of V. mali. The VmHbh protein had high enzymatic activities of hydroxybenzoate hydroxylase, which could convert 4-hydroxybenzoate to protocatechuic acid. These four VmHbh genes all had highly elevated transcript levels during the V. mali infection process, especially VmHbh1 and VmHbh4, with 26.0- and 53.4-fold increases, respectively. Mutants of the four genes were generated to study whether VmHbhs are required for V. mali pathogenicity. Of the four genes, the VmHbh1 and VmHbh4 deletion mutants considerably attenuated V. mali virulence in apple leaves and in twigs, coupled with much reduced toxin levels. The VmHbh2 and VmHbh3 deletion mutants promoted the transcript levels of the other VmHbhs, suggesting functional redundancies of VmHbhs in V. mali virulence. The results provide insights into the functions of VmHbhs in the production of protocatechuic acid by V. mali during its infection of apple trees.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/mpp.13119DOI Listing
August 2021

Associations between borderline personality disorder features and the risk of first onset major depressive disorder: Findings from a 2-year longitudinal study in a sample of first-year university students in China.

J Affect Disord 2021 Aug 8;295:5-10. Epub 2021 Aug 8.

School of Mental Health, Jining Medical University, Jining, China; Institute of Mental Health Research, University of Ottawa, Ottawa, Canada; School of Epidemiology and Public Health, University of Ottawa, Ottawa, Canada. Electronic address:

Background: The objective of this study was to estimate the associations between specific BPD features on the risk of first onset MDD over 2 years and to investigate the roles of sex, baseline depression and anxiety in the associations.

Methods: A longitudinal study of Chinese first-year university students aged between 15 and 24 years and who did not have a lifetime MDD (n = 7,627). Random effect logistic regression modeling was used to estimate the associations between specific BPD features, the number of BPD features, and the risk of MDD.

Results: The feeling of emptiness (OR = 2.14, 95% CI: 1.57, 2.92), paranoid/ dissociation (OR = 2.01, 95% CI: 1.43, 2.82), affective instability (OR = 1.71, 95% CI: 1.22, 2.39) conferred significant risk of MDD. Baseline depression and anxiety interacted with unstable relationship, identity disturbance and paranoid/disassociation in relation to the risk of MDD. A higher number of BPD features is associated with an increased risk of MDD.

Limitations: Data collection relied on self-report. Thus, recall and reporting biases are possible. Due to the pandemic public health restrictions, we had to change the method of data collection at second annual assessment, which resulted in a lower response rate (66.5%).

Conclusions: Mental health professionals should pay attention to the presence of BPD features in youth and young adults, as they are at high risk of developing a MDD in a short time period, and the risk increases with the number of BDP features.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jad.2021.08.008DOI Listing
August 2021

The NDR kinase-MOB complex FgCot1-Mob2 regulates polarity and lipid metabolism in Fusarium graminearum.

Environ Microbiol 2021 Sep 9;23(9):5505-5524. Epub 2021 Aug 9.

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.

Members of the NDR (nuclear Dbf2-related) protein-kinase family are essential for cell differentiation and polarized morphogenesis. However, their functions in plant pathogenic fungi are not well understood. Here, we characterized the NDR kinase FgCot1 and its activator FgMob2 in Fusarium graminearum, a major pathogen causing Fusarium head blight (FHB) in wheat. FgCot1 and FgMob2 formed a NDR kinase-MOB protein complex. Localization assays using FgCot1-GFP or FgMob2-RFP constructs showed diverse subcellular localizations, including cytoplasm, septum, nucleus and hyphal tip. ΔFgcot1 and ΔFgmob2 exhibited serious defects in hyphal growth, polarity, fungal development and cell wall integrity as well as reduced virulence in planta. In contrast, lipid droplet accumulation was significantly increased in these two mutants. Phosphorylation of FgCot1 at two highly conserved residues (S462 and T630) as well as five new sites synergistically contributed its role in various cellular processes. In addition, non-synonymous mutations in two MAPK (mitogen-activated protein kinase) proteins, FgSte11 and FgGpmk1, partially rescued the growth defect of ΔFgmob2, indicating a functional link between the FgCot1-Mob2 complex and the FgGpmk1 signalling pathway in regulating filamentous fungal growth. These results indicated that the FgCot1-Mob2 complex is critical for polarity, fungal development, cell wall organization, lipid metabolism and virulence in F. graminearum.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1462-2920.15698DOI Listing
September 2021

Ultralow Resistance Two-Stage Electrostatically Assisted Air Filtration by Polydopamine Coated PET Coarse Filter.

Small 2021 Aug 26;17(33):e2102051. Epub 2021 Jul 26.

Department of Nuclear Science and Engineering and Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Airborne particulate matters (PM) pose serious health threats to the population, and efficient filtration is needed for indoor and vehicular environments. However, there is an intrinsic conflict between filtration efficiency, air resistance, and service life. In this study, a two-stage electrostatically assisted air (EAA) filtration device is designed and the efficiency-air resistance-filter life envelope is significantly improved by a thin coating of polydopamine (PDA) on the polyethylene terephthalate (PET) coarse filter by in situ dopamine polymerization. The 8 mm thick EAA [email protected] filter has a high filtration efficiency of 99.48% for 0.3 µm particles, low air resistance of 9.5 Pa at a filtration velocity of 0.4 m s , and steady performance up to 30 d. Compared with the bare PET filter, the penetration rate for 0.3 µm particles is lowered by 20×. The coated PDA is of submicron thickness, 10  × the gap distance between filter fibers, so low air resistance could be maintained. The filter shows steadily high filtration efficiency and an acceptable increase of air resistance and holds nearly as many particles as its own weight in a 30 day long-term test. The working mechanism of the EAA coarse filter is investigated, and the materials design criteria are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102051DOI Listing
August 2021

Identification of Mellein as a Pathogenic Substance of by UPLC-MS/MS Analysis and Phytotoxic Bioassay.

J Agric Food Chem 2021 Aug 24;69(30):8471-8481. Epub 2021 Jul 24.

College of Plant Health and Medicine, Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao 266109, P. R. China.

is a pathogenic fungus that can cause apple ring rot, a destructive apple disease in China. There have been reports on its molecular pathogenesis, but the pathogenic substances still remain unknown. In the present study, instrument analysis including UPLC-high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance showed that fermentation broth contained ()-(-)-mellein, a well-known fungal enantiomer of mellein. For further confirmation, a UPLC-MS/MS method for the determination of mellein was developed and validated. By this method, mellein was found to also exist in -infected apple fruits and branches with concentration ranges of 0.14-0.94 and 5.88-80.29 mg/kg, respectively. The concentration in fruits reached a peak at 48 h after pathogen inoculation, while a sustained concentration increase was achieved within 11 days for branches. Simultaneously, it was evident that there was a relation between disease spot expansion and mellein production kinetics in apple tissue. Phytotoxic bioassay showed that mellein could cause discoloration and death of apple leaves and browning in stems. Therefore, we confirmed that mellein was one of the pathogenic substances of . The present study provided additional data for the research on the pathogenesis of this pathogen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.1c03249DOI Listing
August 2021

Heterogeneous Degradation in Thick Nickel-Rich Cathodes During High-Temperature Storage and Mitigation of Thermal Instability by Regulating Cationic Disordering.

Small 2021 Aug 19;17(34):e2102055. Epub 2021 Jul 19.

Shenzhen Key Laboratory on Power Battery Safety Research and Shenzhen Geim Graphene Center, Tsinghua Shenzhen International Graduate School, Shenzhen, 518055, China.

The thermal instability is a major problem in high-energy nickel-rich layered cathode materials for large-scale battery application. Due to the scarce investigation of thick electrodes at the practical full-cell level, the understanding of thermal failure mechanism is still insufficient. Herein, an intrinsic origin of thermal instability in fully charged industrial pouch cells during high-temperature storage is discovered. Through the investigation from crystals to particles, and from electrodes to cells, it is shown that serious top-down heterogeneous degradation occurs along the depth direction of the thick electrode, including phase transition, cationic disordering, intergranular/intragranular cracks, and side reactions. Such degradation originates from the abundant oxygen vacancies and reduced catalytic Ni at cathode surface, causing microstructural defects and directly leading to the thermal instability. Nonmagnetic elements doping and surface modification are suggested to be effective in mitigating the thermal instability through modulating cationic disordering.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202102055DOI Listing
August 2021

Hierarchical Porous Graphene Bubbles as Host Materials for Advanced Lithium Sulfur Battery Cathode.

Front Chem 2021 24;9:653476. Epub 2021 May 24.

Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.

The serious shuttle effect, low conductivity, and large volume expansion have been regarded as persistent obstacles for lithium sulfur (Li-S) batteries in its practical application. Carbon materials, such as graphene, are considered as promising cathode hosts to alleviate those critical defects and be possibly coupled with other reinforcement methods to further improve the battery performance. However, the open structure of graphene and the weak interaction with sulfur species restrict its further development for hosting sulfur. Herein, a rational geometrical design of hierarchical porous graphene-like bubbles (PGBs) as a cathode host of the Li-S system was prepared by employing magnesium oxide (MgO) nanoparticles as templates for carbonization, potassium hydroxide (KOH) as activation agent, and car tal pitch as a carbon source. The synthesized PGBs owns a very thin carbon layer around 5 nm that can be comparable to graphite nanosheets. Its high content of mesoporous and interconnected curved structure can effectively entrap sulfur species and impose restrictions on their diffusion and shuttle effect, leading to a much stable electrochemical performance. The reversible capacity of [email protected] 0.3 C still can be maintained at 831 mAh g after 100 cycles and 512 mAh g after 500 cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2021.653476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181144PMC
May 2021

Measurement of postpartum blood loss using a new two-set liquid collection bag for vaginal delivery: A prospective, randomized, case control study.

Medicine (Baltimore) 2021 May;100(19):e25906

Department of Obstetrics, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.

Background: Postpartum hemorrhage (PPH) is a major obstetric complication, and the real-time measurement of blood loss is important in the management and treatment of PPH. We designed a new two-set liquid collection bag (TSLCB) for measuring postpartum blood loss in vaginal delivery. The aim of this study was to evaluate the effectiveness of the TSLCB in separating the blood from the amniotic fluid during vaginal delivery and in determining the accuracy of the measured postpartum blood loss.

Methods: A prospective, randomized, case control study was conducted in the Women's Hospital, Zhejiang University School of Medicine, from March 2018 to April 2018. Sixty single pregnant women with spontaneous labor at 37-41 weeks without maternal complications were randomly divided into the experimental and control groups. The TSLCB was used to evaluate separately the amount of blood and amniotic fluid. For the control group, visual estimation and traditional plastic blood-collecting consumables were used to estimate the amount of postpartum blood loss. The measured blood loss between the two groups was compared, and the association of the measured blood loss with various clinical lab indices and vital signs was investigated.

Results: The TSLCB (the experimental group) improved the detection of the measured blood loss compared with visual estimation and the traditional method (the control group) (P < .05). In the experimental group, correlation analysis showed that the measured blood loss at delivery and within 24 h of delivery was significantly associated with the decreased hemoglobin level, red blood cell count, and hematocrit level of patients (r = -0.574, -0.455, -0.437; r = 0.-595, -0.368, -0.374; P < .05). In the control group, only the measured blood loss within 24 h of delivery was associated with the decreased hemoglobin level (r = -0.395, P < .05). No blood transfusion and plasma expanders were required in the treatment of PPH for both groups.

Conclusions: The TSLCB can be used to accurately measure the postpartum blood loss in vaginal delivery by medical personnel.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133090PMC
May 2021

Predictive factors associated with disease recurrence in patients with severe intrahepatic cholestasis of pregnancy: a retrospective study of 118 cases.

J Matern Fetal Neonatal Med 2021 May 16:1-8. Epub 2021 May 16.

Department of Obstetrics and Gynecology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Purpose: To compare the clinical characteristics of pregnant women and perinatal outcomes with or without recurrent severe intrahepatic cholestasis of pregnancy (sICP), and identify possible factors associated with disease recurrence.

Methods: A retrospective study of 164,603 deliveries was performed to identify pregnant women diagnosed with sICP in the previous pregnancy from January 2012 to December 2020. Eligible patients were divided into two subgroups according to the status of disease recurrence in the second pregnancy: recurrent severe ICP (r-sICP) and non-recurrent severe ICP (nr-sICP). Demographics, clinical characteristics, maternal and perinatal outcomes, and potential factors linked to disease recurrence were analyzed.

Results: Totally 118 patients were enrolled and respectively classified into the r-sICP group ( = 63) and the nr-sICP group ( = 55). The proportion of hepatitis B virus (HBV) infection (HBsAg+, HBeAg+, HBcAb+) and early-onset ICP (<28 weeks) in the r-sICP group in the previous pregnancy were higher than those in the nr-sICP group. In the second delivery, neonatal outcomes in the r-sICP group were worse than those in the nr-sICP group. Logistic regression analysis of predictive factors for disease recurrence in the second delivery revealed that the combination of HBV infection and early-onset ICP in the previous delivery had the steepest receiver-operating characteristic (ROC) curve value 0.720 (95%CI: 0.629-0.812).

Conclusion: Patients with sICP displayed a higher recurrence rate in the second pregnancy. Being <28 weeks at the time of ICP diagnosis and having HBV infection in the previous delivery appear to be independent predictive factors for disease recurrence of sICP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/14767058.2021.1925645DOI Listing
May 2021

Analysis of maternal and neonatal outcomes using cervical cerclage or conservative treatment in singleton gestations with a sonographic short cervix.

Medicine (Baltimore) 2021 May;100(18):e25767

Department of Obstetrics, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.

Abstract: To investigate the effect of cervical cerclage or conservative treatment on maternal and neonatal outcomes in singleton gestations with a sonographic short cervix, and further compare the relative treatment value.A retrospective study was conducted among women with singleton gestations who had a short cervical length (<25 mm) determined by ultrasound during the period of 14 to 24 weeks' gestation in our institution. We collected clinical data and grouped the patients according to a previous spontaneous preterm birth (PTB) at <34 weeks of gestation or second trimester loss (STL) and sub-grouped according to treatment option, further comparing the maternal and neonatal outcomes between different groups.In the PTB or STL history cohort, the cerclage group had a later gestational age at delivery (35.3 ± 3.9 weeks vs 31.6 ± 6.7 weeks) and a lower rate of perinatal deaths (2% vs 29.3%) compared with the conservative treatment group. In the non-PTB-STL history cohort, the maternal and neonatal outcomes were not significantly different between the cerclage group and conservative treatment group. More importantly, for patients with a sonographic short cervix who received cervical cerclage, there was no significant difference in the maternal and neonatal outcomes between the non-PTB-STL group and PTB or STL group.For singleton pregnant with a history of spontaneous PTB or STL and a short cervical length (<25 mm), cervical cerclage can significantly improve maternal and neonatal outcomes; however, conservative treatment (less invasive and expensive than cervical cerclage) was more suitable for those pregnant women without a previous PTB and STL history.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104303PMC
May 2021

Interfacial Covalent Bonds Regulated Electron-Deficient 2D Black Phosphorus for Electrocatalytic Oxygen Reactions.

Adv Mater 2021 May 3;33(20):e2008752. Epub 2021 May 3.

Center for Advancing Electronics Dresden (cfaed) & Faculty of Chemistry and Food Chemistry, Technische Universität Dresden, Mommsenstrasse 4, Dresden, 01062, Germany.

Developing resource-abundant and sustainable metal-free bifunctional oxygen electrocatalysts is essential for the practical application of zinc-air batteries (ZABs). 2D black phosphorus (BP) with fully exposed atoms and active lone pair electrons can be promising for oxygen electrocatalysts, which, however, suffers from low catalytic activity and poor electrochemical stability. Herein, guided by density functional theory (DFT) calculations, an efficient metal-free electrocatalyst is demonstrated via covalently bonding BP nanosheets with graphitic carbon nitride (denoted BP-CN-c). The polarized PN covalent bonds in BP-CN-c can efficiently regulate the electron transfer from BP to graphitic carbon nitride and significantly promote the OOH* adsorption on phosphorus atoms. Impressively, the oxygen evolution reaction performance of BP-CN-c (overpotential of 350 mV at 10 mA cm , 90% retention after 10 h operation) represents the state-of-the-art among the reported BP-based metal-free catalysts. Additionally, BP-CN-c exhibits a small half-wave overpotential of 390 mV for oxygen reduction reaction, representing the first bifunctional BP-based metal-free oxygen catalyst. Moreover, ZABs are assembled incorporating BP-CN-c cathodes, delivering a substantially higher peak power density (168.3 mW cm ) than the Pt/C+RuO -based ZABs (101.3 mW cm ). The acquired insights into interfacial covalent bonds pave the way for the rational design of new and affordable metal-free catalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202008752DOI Listing
May 2021

Solid Polymer Electrolytes with High Conductivity and Transference Number of Li Ions for Li-Based Rechargeable Batteries.

Adv Sci (Weinh) 2021 Apr 8;8(7):2003675. Epub 2021 Feb 8.

Department of Chemistry and Biochemistry Northern Illinois University DeKalb IL 60115 USA.

Smart electronics and wearable devices require batteries with increased energy density, enhanced safety, and improved mechanical flexibility. However, current state-of-the-art Li-based rechargeable batteries (LBRBs) use highly reactive and flowable liquid electrolytes, severely limiting their ability to meet the above requirements. Therefore, solid polymer electrolytes (SPEs) are introduced to tackle the issues of liquid electrolytes. Nevertheless, due to their low Li conductivity and Li transference number (LITN) (around 10 S cm and 0.5, respectively), SPE-based room temperature LBRBs are still in their early stages of development. This paper reviews the principles of Li conduction inside SPEs and the corresponding strategies to improve the Li conductivity and LITN of SPEs. Some representative applications of SPEs in high-energy density, safe, and flexible LBRBs are then introduced and prospected.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202003675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025011PMC
April 2021

Multi-ion Strategy toward Highly Durable Calcium/Sodium-Sulfur Hybrid Battery.

Nano Lett 2021 Apr 14;21(8):3548-3556. Epub 2021 Apr 14.

Centre for Clean Energy Technology, Faculty of Science, University of Technology Sydney, Sydney, New South Wales 2007, Australia.

Nonlithium (Li) metal-sulfur batteries are a viable technology for large-scale energy storage due to their relative high energy densities and low cost. However, their practical application is still hindered by the insufficient reversibility and/or limited cycling stability. Herein, we report a high-performance calcium/sodium-sulfur (Ca/Na-S) hybrid battery enabled by a multi-ion chemistry. The introduction of Na ions in the electrolyte greatly boosts the conversion of Ca polysulfides, which has been verified by theoretical calculation and experimental investigation. Meanwhile, the presence of Ca ions constructs a protective electrostatic shield around the initial protrusions on the Na metal anode without prereduction, thus efficiently suppressing the Na dendrite growth. The as-developed Ca/Na-S cell exhibited a high reversible capacity of 947 mAh g at 0.1 C with long cycle life, clearly demonstrating the feasibility of this multi-ion strategy for developing low-cost non-Li metal-sulfur batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.1c00448DOI Listing
April 2021

Splenectomy provides protective effects against CLP-induced sepsis by reducing TRegs and PD-1/PD-L1 expression.

Int J Biochem Cell Biol 2021 07 24;136:105970. Epub 2021 Mar 24.

National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China; Shaanxi Provincial Clinical Research Center for Hepatic & Splenic Diseases, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China. Electronic address:

The role of the spleen in sepsis is still controversial. Therefore, we investigated the effect of the spleen on sepsis-induced immune dysfunction in C57BL/6 mice subjected to caecal ligation and puncture (CLP). Changes in different immune cells and apoptotic cells in the spleen and peripheral blood were observed 4, 24 and 48 h after CLP. Then, we determined that 48 h following CLP was the most significant period of immunosuppression. Next, we divided the mice into four groups: control, CLP, CLP + spx (splenectomy 48 h after CLP) and spx + CLP (splenectomy surgery two weeks before CLP). Compared with the CLP mice, the CLP + spx and spx + CLP mice had improved survival rates and organ injuries, increased expression of inflammatory factors, a decreased proportion of regulatory T cells (Tregs), and reduced expression of the genes involved in the programmed cell death 1 and its ligand 1 (PD1-PDL1) pathway in immune cells and T-cell immunoglobulin-mucin domain 3 (Tim 3) and Galectin9 in the liver and lungs after 72 h in late-phase sepsis. In addition, the expression of PD-1 was significantly reduced in T cells in spx + CLP mice, and the expression of PD-L1 in myeloid-derived suppressor cells (MDSCs) was reduced in the CLP + spx group, especially in macrophages. These findings suggested that splenectomy could protect septic mice from exhaustion of immune cells by reducing the proliferation of Treg cells and expression of the PD-1/PD-L1 axis in immune cells during the immunosuppressive stage of sepsis. Splenectomy could also reduce liver and lung injuries possibly via the Tim 3 and/or Galectin-9 axis. The spleen is an important regulator of the occurrence and development of sepsis, which provides a new perspective to improve the prognosis of sepsis by regulating the spleen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biocel.2021.105970DOI Listing
July 2021

The lncRNA Snhg1-Vps13D vesicle trafficking system promotes memory CD8 T cell establishment via regulating the dual effects of IL-7 signaling.

Signal Transduct Target Ther 2021 03 24;6(1):126. Epub 2021 Mar 24.

Institute of Immunology PLA, Third Military Medical University, Chongqing, 400038, China.

The efficient induction and long-term persistence of pathogen-specific memory CD8 T cells are pivotal to rapidly curb the reinfection. Recent studies indicated that long-noncoding RNAs expression is highly cell- and stage-specific during T cell development and differentiation, suggesting their potential roles in T cell programs. However, the key lncRNAs playing crucial roles in memory CD8 T cell establishment remain to be clarified. Through CD8 T cell subsets profiling of lncRNAs, this study found a key lncRNA-Snhg1 with the conserved naive-effector-memory expression pattern in CD8 T cells of both mice and human, that can promote memory formation while impeding effector CD8 in acute viral infection. Further, Snhg1 was found interacting with the conserved vesicle trafficking protein Vps13D to promote IL-7Rα membrane location specifically. With the deep mechanism probing, the results show Snhg1-Vps13D regulated IL-7 signaling with its dual effects in memory CD8 generation, which not just because of the sustaining role of STAT5-BCL-2 axis for memory survival, but more through the STAT3-TCF1-Blimp1 axis for transcriptional launch program of memory differentiation. Moreover, we performed further study with finding a similar high-low-high expression pattern of human SNHG1/VPS13D/IL7R/TCF7 in CD8 T cell subsets from PBMC samples of the convalescent COVID-19 patients. The central role of Snhg1-Vps13D-IL-7R-TCF1 axis in memory CD8 establishment makes it a potential target for improving the vaccination effects to control the ongoing pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-021-00492-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987995PMC
March 2021

Butylated Hydroxytoluene Induced Resistance Against in Apple Fruit.

Front Microbiol 2020 14;11:599062. Epub 2021 Jan 14.

Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.

Apple ring rot caused by is an important disease in China, which leads to serious economic losses during storage. Plant activators are compounds that induce resistance against pathogen infection and are considered as a promising alternative strategy to traditional chemical treatment. In the present study, butylated hydroxytoluene (BHT), a potential plant activator, was evaluated for its induced resistance against in postharvest apple fruits. The physiological and molecular mechanisms involved in induced resistance were also explored. The results showed that BHT treatment could trigger strong resistance in apple fruits against , and the optimum concentration was 200 μmol L by immersion of fruits. BHT treatment significantly increased the activities of four defensive enzymes and alleviated lipid peroxidation by increasing antioxidant enzyme activities. In addition, salicylic acid (SA) content was enhanced by BHT treatment as well as the expression of three SA biosynthesis-related genes (, , and ) and two defense genes ( and ). Our results suggest that BHT-conferred resistance against might be mainly through increasing the activities of defense-related enzymes and activating SA signaling pathway, which may provide an alternative strategy to control apple ring rot in postharvest fruits.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.599062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840594PMC
January 2021

The effects of fipronil exposure on oxidative stress, non-specific immunity, autophagy, and apoptosis in the common carp.

Environ Sci Pollut Res Int 2021 Jun 30;28(22):27799-27810. Epub 2021 Jan 30.

College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.

The increase in the area treated with the insecticide fipronil has caused concern for aquatic organisms such as fish. Here, we assessed the effect of fipronil on carp indexes of non-specific immunity, oxidative stress, autophagy, and apoptosis following exposure to 0.074 mg/L and 0.185 mg/L of fipronil in the aqueous environment for 1 day, 3 days, 5 days, and 7 days. It was found that glutathione (GSH), malonaldehyde (MDA), and superoxide dismutase (SOD) in gills were significantly reduced (P < 0.05). The increase in exposure time increases the impact on GSH, SOD, and MDA parameters in the liver and intestine. Liver acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM) activity levels increased significantly in the treatment group on the first day after exposure, except for the 0.074 mg/L group of ACP (P < 0.05). The mRNA expression levels of autophagy-related genes ATG12, ATG5, ATG16L, LC3-II, and BECN1 were generally elevated in the liver and intestine during the initial exposure period (P < 0.05), while mTOR was significantly reduced on the first and third days after treatment (P < 0.05). From the results of Western blotting (WB), we can see that the amount of LC3-II was significantly higher than that of LC3-I at 1, 3, and 5 days of exposure (P < 0.05). Furthermore, the apoptosis-related gene Bcl-2 reached its peak in the liver, intestine, and gill on the first day, and caspase3 was significantly downregulated throughout the exposure period (P < 0.05). The results showed that fipronil was potentially harmful to carp and should be used moderately to reduce the damage to aquatic ecosystems. This study complements the mechanism theory of fipronil on fish toxicology and has a certain value for human health risk assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-12573-xDOI Listing
June 2021

Progression of symptoms caused by Botryosphaeria dothidea on apple branches.

Phytopathology 2021 Jan 24. Epub 2021 Jan 24.

Qingdao Agricultural University, 98431, College of Plant Health and Medicine, Qingdao, Shandong, China;

Until recently, the causal agent of Botryosphaeria canker was assumed to differ from that causing ring rot on fruit and warts on branches on apple trees in China and East Asia. However, recent research documented that Botryosphaeria dothidea caused both disease symptoms on apple. Inoculations with strains isolated from cankers and warts on branches were conducted to investigate symptom progression caused by B. dothidea and conditions inducing the two symptom types. The results confirmed that both cankers and warts are caused by B. dothidea. Warts are the results of hyperplasia and suberization of bark tissues induced by fungal infection, whereas cankers result from the rapid growth of hyphae from inside warts, lenticels or wounds. Resistance to B. dothidea exists in living apple branches. When a living branch is infected via lenticels, the pathogen induces proliferation and suberization of cortical cells that restricts the growth and expansion of the hyphae, leading to wart symptom. However, under certain stress conditions such as drought, the hyphae inside host tissues expand rapidly and kill cortical cells, leading to canker development. Host resistance may recover during active growth periods, which suppresses or even stops rapid expansion of the hyphae, leading to the intermediate symptom of canker warts. Abiotic factors, such as drought or high temperature in early spring, can result in rapid extension of colonized hyphae in branches and conversion of warts to cankers. Preventing this transition can be an important measure in managing Botryosphaeria canker on apple.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PHYTO-12-20-0551-RDOI Listing
January 2021

Role of prenatal imaging in the diagnosis and management of fetal facio-cervical masses.

Sci Rep 2021 01 14;11(1):1385. Epub 2021 Jan 14.

Department of Radiology, Women's Hospital, Zhejiang University School of Medicine, Xueshi Rd No. 1, Hangzhou, Zhejiang, People's Republic of China.

Congenital facio-cervical masses can be a developmental anomaly of cystic, solid, or vascular origin, and have an inseparable relationship with adverse prognosis. This retrospective cross-sectional study aimed at determining on the prenatal diagnosis of congenital facio-cervical masses, its management and outcome in a large tertiary referral center. We collected information on prenatal clinical data, pregnancy outcomes, survival information, and final diagnosis. Out of 130 cases of facio-cervical masses, a total of 119 cases of lymphatic malformations (LMs), 2 cases of teratoma, 2 cases of thyroglossal duct cyst, 4 cases of hemangioma, 1 case of congenital epulis, and 2 cases of dermoid cyst were reviewed. The accuracy of prenatal ultrasound was 93.85% (122/130). Observations of diameters using prenatal ultrasound revealed that the bigger the initial diameter is, the bigger the relative change during pregnancy. Magnetic resonance imaging (MRI) revealed that 2 cases of masses were associated with airway compression. In conclusion, ultrasound has a high overall diagnostic accuracy of fetal face and neck deformities. Prenatal US can enhance the management of ambulatory monitoring and classification. Furthermore, MRI provided a detailed assessment of fetal congenital malformations, as well as visualization of the trachea, presenting a multi-dimensional anatomical relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-80976-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809128PMC
January 2021

Preoperative predictive factor analysis of ovarian malignant involvement in premenopausal patients with clinical stage I endometrioid endometrial carcinoma.

Sci Rep 2021 01 13;11(1):1219. Epub 2021 Jan 13.

Department of Gynecologic Oncology, Women's Hospital, Zhejiang University School of Medicine, Xueshi Road 1, Hangzhou, 310006, Zhejiang, People's Republic of China.

Earlier literature suggests that ovarian preservation in young premenopausal clinical stage I endometrioid endometrial carcinoma patients does not negatively impact prognosis. The main purpose of this study was to clarify the incidence of ovarian malignant involvement in this group and further identify potential preoperative predictive factors of ovarian malignant involvement. A total of 511 premenopausal (age ≤ 50 years) patients were enrolled for the study at Women's Hospital, Zhejiang University School of Medicine, between January 2002 and December 2016. Ovarian malignant involvements were detected in 23 of the patients (4.5%). Univariate and multivariate logistic analysis validated preoperative imaging of myometrial invasion depth and preoperative serum carbohydrate antigen 125 (CA125) level as independent risk predictors of postoperative ovarian malignant involvement. Receiver operating characteristic (ROC) curves was generated for a combination of the two factors. The area under curve (AUC) was 0.772 (95% confidence interval [CI] 0.661-0.884) for the combined two factors. The incidence of postoperative ovarian malignant involvement was relatively minimal. Preoperative imaging of myometrial invasion depth and serum CA125 level were independent risk predictors of ovarian malignant involvement. These findings may facilitate preoperative counseling of patients and informed clinical decision-making on ovarian preservation in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-78953-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806745PMC
January 2021

TWIST1 expression and clinical significance in type I endometrial cancer and premalignant lesions: A retrospective clinical study.

Medicine (Baltimore) 2020 Nov;99(48):e23397

Department of Gynecologic Oncology.

The aim of this study was to assess the correlation of TWIST1 expression with clinical parameters and the prognosis of type I endometrial cancer (EC).This retrospective study enrolled 345 patients. Immunohistochemical staining was performed on 55 normal endometrium (NE) samples, 27 atypical hyperplasia (AH) samples, and 263 type I EC samples. The association between TWIST1 staining and clinical characteristics and survival was evaluated by univariate and multivariate analyses.We found significantly higher TWIST1 expression in patients with AHs and type I ECs than NEs, but there was no significant difference between TWIST1 expression in AHs and type I ECs. Aberrant TWIST1 expression was significantly associated with clinical parameters, indicating poor prognosis and shorter patient survival. Pearsons Chi-Squared test showed that high TWIST1 expression was significantly associated with a shorter disease-free survival and overall survival. More importantly, multivariate analysis showed that high TWIST1 expression, in addition to myometrial invasion, lymph vascular space invasion, and lymph node metastasis, was an independent predictor of worse DFS in patients with type I ECs.Our findings suggest that TWIST1 might be useful in diagnosing ECs and predicting prognosis in patients with AHs and type I ECs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000023397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710231PMC
November 2020

Biocontrol efficiency of Meyerozyma guilliermondii Y-1 against apple postharvest decay caused by Botryosphaeria dothidea and the possible mechanisms of action.

Int J Food Microbiol 2021 Jan 6;338:108957. Epub 2020 Nov 6.

College of Plant Health and Medicine, Key Laboratory of Integrated Crop Pest Management of Shandong Province, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, Shandong 266109, China. Electronic address:

Apple ring rot, caused by Botryosphaeria dothidea, is one of the important diseases in China. This pathogen infects branches and fruit and also results in fruit decay during storage. Biocontrol agents have been proposed to reduce apple decays during storage and are considered as a promising alternative strategy to traditional chemical treatment. In this study, Meyerozyma guilliermondii Y-1, isolated from healthy grape fruit, was firstly evaluated for its biocontrol efficiency against B. dothidea in postharvest apple fruit, and the possible mechanisms were investigated. The results revealed that M. guilliermondii Y-1 treatment effectively reduced apple ring rot caused by B. dothidea in vivo. The disease incidence and lesion diameter were reduced by 32.22% and 57.51% compared with those of control fruit. Furthermore, the use of filtrate and autoclaved culture of M. guilliermondii Y-1 also showed a certain degree of control efficiency against fruit ring rot. M. guilliermondii Y-1 significantly inhibited the mycelial growth and spore generation of B. dothidea in vitro and exhibited an obvious ability to colonize in apple fruit wounds and surface at 25 °C or 4 °C. In addition, M. guilliermondii Y-1 treatment significantly enhanced the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), phenylalanine ammonialyase (PAL), and polyphenoloxidase (PPO), promoted the total phenolics content, and alleviated lipid peroxidation in apple fruit. As expected, we found that the expression of four pathogenesis-related proteins genes (MdPR1, MdPR5, MdGLU, and MdCHI) was remarkably increased by M. guilliermondii Y-1 treatment. Our data together suggest that M. guilliermondii Y-1 is a potential biocontrol agent against B. dothidea postharvest infection in apple fruit, partially through inhibiting mycelial growth and spore germination of B. dothidea, competing for space and nutrient with pathogen, and inducing resistance in apple fruit by stimulating a series of defense responses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108957DOI Listing
January 2021

Gene Methylation as a Noninvasive Marker for Hepatocellular Carcinoma.

Dis Markers 2020 29;2020:6289063. Epub 2020 Oct 29.

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Invasion and Metastasis Research and National Clinical Research Centre for Digestive Diseases Beijing 100050, China.

Background: Early detection appears to be the most effective approach to improve the overall survival of patients with hepatocellular carcinoma (HCC). We evaluated the potential performance of plasma methylation (mSEPT9) as a noninvasive biomarker for the diagnosis of patients with HCC.

Methods: A total of 373 subjects were included, and the group consisted of 104 HCC patients, 95 with an at-risk disease, and 174 healthy controls (HC). The methylation of mSEPT9 was determined using methylation-specific fluorescence quantitative PCR. The diagnostic performance of plasma mSEPT9 for HCC was assessed in a single-blind manner.

Results: The receiver operating characteristic (ROC) curve showed that plasma mSEPT9 can be used to detect and discriminate HCC with an area under the ROC curve (AUROC) of 0.961, a sensitivity of 82.7%, and specificity of 96.0% from HC. These results showed that plasma mSEPT9 had better diagnostic performance than serum alpha fetoprotein (AFP) (AUROC 0.881, sensitivity 57.7%, and specificity 98.3%). Similar results were noted in the detection of early-stage HCC. When combined with serum AFP, the sensitivity increased to 91.3% and 87.7% for the detection of HCC and early-stage HCC,respectively. Notably, the levels of plasma mSEPT9 dramatically decreased after surgery ( = 0.001).

Conclusions: Plasma methylation might serve as a useful and noninvasive biomarker for the diagnosis of HCC and can be used to evaluate the therapeutic efficacy of HCC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/6289063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647768PMC
August 2021

Rational design of layered oxide materials for sodium-ion batteries.

Science 2020 11;370(6517):708-711

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Sodium-ion batteries have captured widespread attention for grid-scale energy storage owing to the natural abundance of sodium. The performance of such batteries is limited by available electrode materials, especially for sodium-ion layered oxides, motivating the exploration of high compositional diversity. How the composition determines the structural chemistry is decisive for the electrochemical performance but very challenging to predict, especially for complex compositions. We introduce the "cationic potential" that captures the key interactions of layered materials and makes it possible to predict the stacking structures. This is demonstrated through the rational design and preparation of layered electrode materials with improved performance. As the stacking structure determines the functional properties, this methodology offers a solution toward the design of alkali metal layered oxides.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.aay9972DOI Listing
November 2020

Effects of the injection compression process parameters on residual stress of plastic lenses.

Appl Opt 2020 Oct;59(30):9626-9632

Residual stress affects significantly the quality of plastic lenses. In this paper, the influence of process parameters on the residual stress of injection compression molded plastic lenses was investigated by orthogonal simulation. The results show that the effect of compression delay time on residual stress was the most significant, followed by compression distance and compression speed, which are both related to the compression process. Then, the relationship between the residual stress obtained by simulations and the optical path difference measured by the experiment was compared by single factor experiments of four key injection compression molding process parameters. The change trends were similar, which proves that the numerical simulation has the potential to predict the residual stress of plastic lenses and optimize the process parameters, so as to improve their optical quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.404024DOI Listing
October 2020

Efficient Construction of a C Interlayer for Mechanically Robust, Dendrite-free, and Ultrastable Solid-State Batteries.

iScience 2020 Oct 1;23(10):101636. Epub 2020 Oct 1.

Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, China.

Interfacial instability between solid electrolytes (SEs) and lithium metal remains a daunting challenge for solid-sate batteries. Here, a conformal C interlayer is efficiently constructed on LiAlGe(PO) (LAGP) SEs by physical vapor deposition, and an ideal interfacial contact is achieved via forming an ionically conducting matrix of LiC with lithium metal. The obtained LiC is beneficial to hinder the growth of lithium dendrites at interface and release the local stress during the lithiation and delithiation. As a result, the Li/LAGP-C/Li symmetric cells demonstrate ultra-stable cycling performance for more than 4,500 h at a current density of 0.034 mA cm. The Li/LAGP-C/LiFePO full cells deliver a reversible capacity of 152.4 mAh g at room temperature, and the capacity retention rate is 85% after more than 100 cycles. This work provides a feasible and scalable strategy to improve the SEs/Li interface for high-performance solid-state batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2020.101636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569341PMC
October 2020

Evaluation of Pyraclostrobin as an Ingredient for Soybean Seed Treatment by Analyzing its Accumulation-Dissipation Kinetics, Plant-Growth Activation, and Protection Against .

J Agric Food Chem 2020 Oct 20;68(43):11928-11938. Epub 2020 Oct 20.

College of Plant Health and Medicine, Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao 266109, P. R. China.

Seed treatment with fungicides has been regarded as a principal, effective, and economic technique for soybean [ .] against pathogenic microorganisms during seed germination and seedling growth. Investigation of the characteristics of seed-treatment reagents is an indispensable basis for their application. The aim of the present work is to evaluate the use of pyraclostrobin as an ingredient for soybean seed treatment by investigating its accumulation-dissipation kinetics in plants, plant-growth activation, and protection against . The results showed that the pyraclostrobin stimulated the visible growth (root and shoot length) of soybean plants, increased the chlorophyll level and root activity, and lowered the malonaldehyde (MDA) level. The peak level and bioavailability of pyraclostrobin in soybean roots were 19.9- and 33.2-fold those in leaves, respectively, indicating that pyraclostrobin was mainly accumulated in roots. Pyraclostrobin had a continuous positive effect on the flavonoid levels and the phenylalanine ammonialyase (PAL) activity in roots and leaves, which could enhance the plant defense system. Pyraclostrobin showed toxicity to with a half-inhibition concentration (EC) of 1.59 and 1.24 μg/mL for pyraclostrobin and pyraclostrobin plus salicylhydroxamic acid (SHAM, an inhibitor of the alternative pathway of respiration), respectively. Seed treatment with pyraclostrobin significantly reduced the severity of Phytophthora root rot, with a control efficacy of 60.7%. To the best of our knowledge, this is the first report on the characteristics of pyraclostrobin used in soybean seed treatment and its efficacy against Phytophthora root rot.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c04376DOI Listing
October 2020

Impact of chronic exposure to the ionic liquid ([C8mim][PF6]) on intestinal physical barrier, immunological barrier and gut microbiota in common carp (Cyprinus carpio L.).

Environ Res 2020 10 14;189:109919. Epub 2020 Jul 14.

College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China. Electronic address:

Ionic liquids (ILs) are commonly known as "green" solvents and have been widely used in various fields. However, the ecotoxicity of ILs in aquatic environment has received considerable attention from scientific researchers. This study investigated the toxic effects of different concentrations of 1-octyl-3-methylimidazolium hexafluorophosphate ([C8mim][PF6]) (0, 1.35, 2.70 and 5.40 mg/L) on intestinal physical barrier, immunological barrier, and intestinal microbiome in common carp on days 30 and 60. The results showed that ([C8mim][PF6]) exposure could reduce the intestinal villus height, decrease the mRNA expression of tight junction genes (occludin, claudin-2 and zonula occludens-1), and increase the levels of D-lactic and diamine oxidase, and reduce acid phosphatase and lysozyme activities, complement 3 and 4 contents, and anti-inflammatory cytokine TGF-β protein level, while increase pro-inflammatory cytokines TNF-α and IL-1β protein levels in common carp. Moreover, ([C8mim][PF6]) exposure was also found to significantly reduce gut microbial diversity and alter microbial community structure in common carp. Collectively, our study highlighted that exposure to ([C8mim][PF6]) could disrupt intestinal physical barrier, impair immunological barrier and alter intestinal microbiome in common carp, suggesting that ILs exert a negative effect on fish intestinal health status and may pose serious health risks in fish. The results of this study may be helpful to illuminate the toxicity mechanisms of the ILs on fish.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2020.109919DOI Listing
October 2020
-->