Publications by authors named "Bao Zhang"

484 Publications

Deeply Mining a Universe of Peptides Encoded by Long Noncoding RNAs.

Mol Cell Proteomics 2021 Jun 12:100109. Epub 2021 Jun 12.

Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Many small open reading frames (smORFs) embedded in lncRNA transcripts have been shown to encode biologically functional polypeptides (smORFs-encoded polypeptides, SEPs) in different organisms. Despite significant advances in genomics, bioinformatics and proteomics that largely enabled the discovery of novel SEPs, their identification across different biological samples is still hampered by their poor predictability, diminutive size and low relative abundance. Here, we take advantage of NONCODE, a repository containing the most complete collection and annotation of lncRNA transcripts from different species, to build a novel database that attempts to maximize a collection of SEPs from human and mouse lncRNA transcripts. In order to further improve SEP discovery, we implemented two effective and complementary polypeptide enrichment strategies, 30 kDa MWCO filter and C8 SPE column. These combined strategies enabled us to discover 353 and 409 SEPs from, respectively, 8 human cell lines, and 3 mouse cell lines and 8 mouse tissues. Importantly, nineteen of the identified SEPs were then verified through in-vitro expression, immunoblotting, parallel reaction monitoring (PRM) and synthetic peptides. Subsequent bioinformatic analysis revealed that some of the physical and chemical properties of these novel SEPs, including amino acid composition and codon usage, are different from those commonly found in canonical proteins. Intriguingly, nearly 65% of the identified SEPs were found to be initiated with non-AUG start codons. Overall, the strategy presented in this study encompasses an efficient workflow that enabled us to identify 762 novel SEPs across multiple cell lines and tissues, which probably represents the largest number of SEPs detected by mass spectrometry reported to date. These novel SEPs might not only provide new clues for the annotation of noncoding elements in the genome but can also serve as a valuable resource for the functional characterization of individual SEPs.
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http://dx.doi.org/10.1016/j.mcpro.2021.100109DOI Listing
June 2021

Hengshun Aromatic Vinegar Ameliorates Vascular Endothelial Injury Regulating PKCζ-Mediated Oxidative Stress and Apoptosis.

Front Nutr 2021 28;8:635232. Epub 2021 May 28.

College of Pharmacy, Dalian Medical University, Dalian, China.

Vascular endothelial injury (VEI) is an early event of atherosclerosis, and reversing endothelial dysfunction has become a new trend in the prevention and treatment of cardiovascular diseases. Hengshun aromatic vinegar (HSAV), a traditional vinegar, has been reported to have many pharmacological activities, but its effect against VEI and the molecular mechanism are still unknown. In this study, effects of HSAV on VEI were evaluated in HO-induced human umbilical vein endothelial cells (HUVECs) and methionine-induced VEI in rats. Results showed that HSAV significantly increased cell viability, inhibited apoptosis, and reduced the generation of reactive oxygen species (ROS) in HO-induced HUVECs. Meanwhile, HSAV decreased serum homocysteine (Hcy), endothelin 1 (ET-1), and oxidized low-density lipoprotein (ox-LDL) levels, increased nitric oxide (NO) and endothelin nitric oxide synthase (eNOS) levels, ameliorated pathological changes in rats with VEI induced by methionine. In parallel, HSAV relieved oxidative stress by decreasing malondialdehyde (MDA) level and increasing superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels in rats with VEI. Mechanism studies indicated that HSAV markedly downregulated the expression of protein kinase C zeta (PKCζ), and consequently regulated sirtuin 1 (Sirt1)-mediated oxidative stress signal pathway, and protein inhibitor of activated STATy (PIASy)-mediated apoptosis pathway, leading to the alleviation of oxidative stress and inhibition of apoptosis. These regulative effects of HSAV were further validated by knockdown and overexpression of PKCζ . In conclusion, HSAV showed protective effect against VEI by inhibiting PKCζ and, thereby, ameliorating oxidative stress and inhibiting apoptosis. This study not only provides guidance for the consumption of vinegar in daily life but also promotes the development of diet supplement for disease prevention.
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http://dx.doi.org/10.3389/fnut.2021.635232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193096PMC
May 2021

An Effector Protein EspF May Induce Host DNA Damage via Interaction With SMC1.

Front Microbiol 2021 26;12:682064. Epub 2021 May 26.

Department of Microbiology, School of Public Health, Southern Medical University, Guangzhou, China.

Enterohemorrhagic (EHEC) O157: H7 is an important foodborne pathogen that causes human diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. EspF is one of the most important effector proteins injected by the Type III Secretion System. It can target mitochondria and nucleoli, stimulate host cells to produce ROS, and promote host cell apoptosis. However, the mechanism of the host-pathogen interaction leading to host oxidative stress and cell cytotoxic effects such as DNA damage remains to be elucidated. Here, we used Cell Counting Kit-8 (CCK-8) assays and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG) ELISA to study cell viability and DNA oxidative damage level after exposure to EspF. Western blot and immunofluorescence were also used to determine the level of the DNA damage target protein p-H2AX and cell morphology changes after EspF infection. Moreover, we verified the toxicity in intestinal epithelial cells mediated by EspF infection . In addition, we screened the host proteins that interact with EspF using CoIP-MS. We found that EspF may more depend on its C-terminus to interact with SMC1, and EspF could activate SMC1 phosphorylation and migrate it to the cytoplasm. In summary, this study revealed that EspF might mediate host cell DNA damage and found a new interaction between EspF and the DNA damage repair protein SMC1. Thus, EspF may mediate DNA damage by regulating the subcellular localization and phosphorylation of SMC1.
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http://dx.doi.org/10.3389/fmicb.2021.682064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188558PMC
May 2021

PGC-1 Protects against Hepatic Ischemia Reperfusion Injury by Activating PPAR and PPAR and Regulating ROS Production.

Oxid Med Cell Longev 2021 19;2021:6677955. Epub 2021 May 19.

Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Peroxisome proliferator-activated receptors (PPARs) and have been shown to be protective in hepatic ischemia/reperfusion (I/R) injury. However, the precise role of PPAR coactivator-1 (PGC-1), which can coactivate both of these receptors, in hepatic I/R injury, remains largely unknown. This study was designed to test our hypothesis that PGC-1 is protective during hepatic I/R injury in vitro and in vivo. Our results show that endogenous PGC-1 is basally expressed in normal livers and is moderately increased by I/R. Ectopic PGC-1 protects against hepatic I/R and hepatocyte anoxia/reoxygenation (A/R) injuries, whereas knockdown of endogenous PGC-1 aggravates such injuries, as evidenced by assessment of the levels of serum aminotransferases and inflammatory cytokines, necrosis, apoptosis, cell viability, and histological examination. The EMSA assay shows that the activation of PPAR and PPAR is increased or decreased by the overexpression or knockdown of PGC-1, respectively, during hepatic I/R and hepatocyte A/R injuries. In addition, the administration of specific antagonists of either PPAR (MK886) or PPAR (GW9662) can effectively decrease the protective effect of PGC-1 against hepatic I/R and hepatocyte A/R injuries. We also demonstrate an important regulatory role of PGC-1 in reactive oxygen species (ROS) metabolism during hepatic I/R, which is correlated with the induction of ROS-detoxifying enzymes and is also dependent on the activations of PPAR and PPAR. These data demonstrate that PGC-1 protects against hepatic I/R injury, mainly by regulating the activation of PPAR and PPAR. Thus, PGC-1 may be a promising therapeutic target for the protection of the liver against I/R injury.
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http://dx.doi.org/10.1155/2021/6677955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159639PMC
May 2021

Genetic Polymorphism of 24 Autosomal STR in the Population of Rwanda.

Biochem Genet 2021 Jun 7. Epub 2021 Jun 7.

College of Medicine & Forensic, Health Science Center, Xi'an Jiao Tong University, 76 Yan Ta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.

Rwanda is one of the smallest countries of Africa, where forensic genetic studies are rarely being conducted and very few DNA databases have been developed. Short tandem repeats (STRs) polymorphisms were investigated in 505 unrelated Rwandese by using the HUMDNA TYPING (Yanhuang) Kit. The following STRs were targeted: D3S1358, D13S317, D7S820, D16S539, SE33, D10S1248, D5S818, D21S11, TPOX, D1S1656, D6S1043, D19S433, D22S1045, D8S1179, Penta E, D2S441, D12S391, D2S1338, vWA, Penta D, TH01, D18S51, CSF1PO and FGA. The purpose of this study was to elucidate the genetic diversity and explore the potential of applying these 24 STR in 505 Rwandan population in forensics. A total of 360 alleles, with corresponding allele frequencies in the range from 0.001 to 0.442, were found in the Rwandan population. SE33 presented the highest polymorphism (PIC=0.921) among these 24 loci, whereas D13S317 presented the lowest one (PIC=0.671). No deviation from the Hardy-Weinberg equilibrium was observed for any of the 24 loci. The forensic parameters, including the combined power of discrimination (PD and the combined exclusion power, have demonstrated that this panel of 24 STRs is highly informative and useful for forensic applications such as individuals' identification and paternity tests. Additionally, the genetic distances between Rwanda population and other 24 published populations were calculated based on 8 overlapping loci with the polygenetic tree revealing significant clusters in the populations associated with their geographic locations and their historical relationship.
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http://dx.doi.org/10.1007/s10528-021-10067-6DOI Listing
June 2021

Identification of differentially expressed genes in diabetic kidney disease by RNA-seq analysis of venous blood platelets.

FEBS Open Bio 2021 May 24. Epub 2021 May 24.

Division of Nephrology, Shanghai Pudong Hospital, Fudan University, Pudong Medical Center, 2800 Gong Wei Road, Shanghai, China.

Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease (ESRD). However, due to shared complications between DKD and chronic kidney disease (CKD), the description and characterization of DKD remain ambiguous in the clinic, hindering the diagnosis and treatment of early stage DKD patients. Although estimated glomerular filtration rate (eGFR) and albuminuria are well-established biomarkers of DKD, early stage DKD is rarely accompanied by high eGFR, and thus there is a need for new sensitive biomarkers. Transcriptome profiling of kidney tissue has been reported previously, but RNA sequencing analysis of the venous blood platelets in DKD patients has not yet been described. In this study, we performed RNA sequencing analysis of venous blood platelets from 3 patients with CKD, 5 patients with DKD, and 10 healthy controls (HC), and compared the results with a CKD-related microarray dataset. In total, 2097 genes with differential transcript levels (GDTLs) were identified in platelets of DKD patients and HC, and 462 GDTLs were identified in platelets of DKD patients and CKD patients. Through KEGG pathway enrichment analysis, we selected 11 pathways, from which 9 potential biomarkers (IL-1B, CD-38, CSF1R, PPARG, NR1H3, DDO, HDC, DPYS and CAD) were identified. Furthermore, by comparing the RNA-seq results with the GSE30566 dataset, we found that the biomarker KCND3 was the only up-regulated gene in DKD patients. These biomarkers may have potential application for the therapy and diagnosis of DKD, as well aiding elucidation of the mechanisms underlying DKD.
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http://dx.doi.org/10.1002/2211-5463.13199DOI Listing
May 2021

A universal strategy towards high-energy aqueous multivalent-ion batteries.

Nat Commun 2021 May 17;12(1):2857. Epub 2021 May 17.

Centre for Clean Energy Technology, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.

Rechargeable multivalent metal (e.g., Ca, Mg or, Al) batteries are ideal candidates for large-scale electrochemical energy storage due to their intrinsic low cost. However, their practical application is hampered by the low electrochemical reversibility, dendrite growth at the metal anodes, sluggish multivalent-ion kinetics in metal oxide cathodes and, poor electrode compatibility with non-aqueous organic-based electrolytes. To circumvent these issues, here we report various aqueous multivalent-ion batteries comprising of concentrated aqueous gel electrolytes, sulfur-containing anodes and, high-voltage metal oxide cathodes as alternative systems to the non-aqueous multivalent metal batteries. This rationally designed aqueous battery chemistry enables satisfactory specific energy, favorable reversibility and improved safety. As a demonstration model, we report a room-temperature calcium-ion/sulfur| |metal oxide full cell with a specific energy of 110 Wh kg and remarkable cycling stability. Molecular dynamics modeling and experimental investigations reveal that the side reactions could be significantly restrained through the suppressed water activity and formation of a protective inorganic solid electrolyte interphase. The unique redox chemistry of the multivalent-ion system is also demonstrated for aqueous magnesium-ion/sulfur||metal oxide and aluminum-ion/sulfur||metal oxide full cells.
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http://dx.doi.org/10.1038/s41467-021-23209-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128864PMC
May 2021

Stability and bioaccessibility of curcumin emulsions stabilized by casein hydrolysates after maleic anhydride acylation and pullulan glycation.

J Dairy Sci 2021 May 10. Epub 2021 May 10.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, P. R. China; School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, P. R. China; State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, P. R. China. Electronic address:

The effects of maleic anhydride (MA) acylation and pullulan glycation on casein hydrolysates (CH) and the physicochemical stability of modified or unmodified CH-stabilized emulsions were explored. Compared with casein, the solubility of CH was improved, and CH (hydrolysis degree 4%) exhibited the optimal emulsifying properties. After the acylation of MA, degrees of acylation (DA) increased with increasing addition of MA. Fourier-transform infrared spectroscopy revealed that a covalent bond was formed between MA and CH. The results of pullulan glycation indicated that the degree of glycation decreased with increasing DA. Acylation combined with glycation effectively reduced the surface hydrophobicity of CH. Results of analysis of physicochemical stability and gastrointestinal fate of curcumin in emulsions revealed that CH modified by MA acylation and pullulan glycation played a positive role in enhancing the stability and bioaccessibility of curcumin loaded in emulsions.
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http://dx.doi.org/10.3168/jds.2020-19613DOI Listing
May 2021

Hengshun Aromatic Vinegar Improves Glycolipid Metabolism in Type 2 Diabetes Mellitus Regulating PGC-1α/PGC-1β Pathway.

Front Pharmacol 2021 26;12:641829. Epub 2021 Apr 26.

College of Pharmacy, Dalian Medical University, Dalian, China.

Hengshun aromatic vinegar (HSAV), produced by typical solid-state or liquid-state fermentation techniques, is consumed worldwide as a food condiment. HSAV shows multiple bioactivities, but its activity in type 2 diabetes mellitus (T2DM) and possible mechanisms have not been reported. In this study, the effects of HSAV against T2DM were evaluated in insulin-induced HepG2 cells and high-fat diet (HFD) and streptozotocin (STZ) induced T2DM rats. Then, the mechanisms of HSAV against T2DM were explored by Real-time PCR, Western blot, immunofluorescence assays, siRNA transfection and gene overexpression experiments. Results indicated that HSAV significantly improved glucose consumption and reduced triglycerides (TG) contents in metabolic disordered HepG2 cells. Meanwhile, HSAV obviously alleviated general status, liver and kidney functions of T2DM rats, and decreased hyperglycemia and hyperlipidemia, improved insulin resistance, and reduced lipid accumulation in liver. Mechanism studies indicated that HSAV markedly down-regulated the expression of proliferator-activated receptor γ coactivator-1α (PGC-1α), then regulated peroxisome proliferators-activated receptor α (PPAR-α)/protein kinase B (AKT) signal pathway mediated gluconeogenesis and glycogen synthesis. Meanwhile, HSAV significantly up-regulated proliferator-activated receptor γ coactivator-1β (PGC-1β), and subsequently decreased sterol regulatory element binding protein-1c (SREBP-1c) pathway mediated lipogenesis. In conclusion, HSAV showed potent anti-T2DM activity in ameliorating dysfunction of glycolipid metabolism through regulating PGC-1α/PGC-1β pathway, which has a certain application prospect as an effective diet supplement for T2DM therapy in the future.
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http://dx.doi.org/10.3389/fphar.2021.641829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109051PMC
April 2021

Fluorinated interphase enables reversible aqueous zinc battery chemistries.

Nat Nanotechnol 2021 May 10. Epub 2021 May 10.

Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, USA.

Metallic zinc is an ideal anode due to its high theoretical capacity (820 mAh g), low redox potential (-0.762 V versus the standard hydrogen electrode), high abundance and low toxicity. When used in aqueous electrolyte, it also brings intrinsic safety, but suffers from severe irreversibility. This is best exemplified by low coulombic efficiency, dendrite growth and water consumption. This is thought to be due to severe hydrogen evolution during zinc plating and stripping, hitherto making the in-situ formation of a solid-electrolyte interphase (SEI) impossible. Here, we report an aqueous zinc battery in which a dilute and acidic aqueous electrolyte with an alkylammonium salt additive assists the formation of a robust, Zn-conducting and waterproof SEI. The presence of this SEI enables excellent performance: dendrite-free zinc plating/stripping at 99.9% coulombic efficiency in a Ti||Zn asymmetric cell for 1,000 cycles; steady charge-discharge in a Zn||Zn symmetric cell for 6,000 cycles (6,000 h); and high energy densities (136 Wh kg in a Zn||VOPO full battery with 88.7% retention for >6,000 cycles, 325 Wh kg in a Zn||O full battery for >300 cycles and 218 Wh kg in a Zn||MnO full battery with 88.5% retention for 1,000 cycles) using limited zinc. The SEI-forming electrolyte also allows the reversible operation of an anode-free pouch cell of Ti||ZnVOPO at 100% depth of discharge for 100 cycles, thus establishing aqueous zinc batteries as viable cell systems for practical applications.
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http://dx.doi.org/10.1038/s41565-021-00905-4DOI Listing
May 2021

Conscious sedation compared to general anesthesia for intracranial mechanical thrombectomy: A meta-analysis.

Brain Behav 2021 May 7:e02161. Epub 2021 May 7.

Department of Anesthesiology, The Fourth Hospital of Shijiazhuang, Shijiazhuang, China.

Introduction: Endovascular therapy is the standard of care for severe acute ischemic stroke caused by large-vessel occlusion in the anterior circulation, but there is a debate on the optimal anesthetic approach during this therapy. Meta-analyses of observational studies suggest that general anesthesia increases disability and death compared with conscious sedation However, their results are conflicting. This meta-analysis study was performed to assess the relationship between the effects of general anesthesia compared to conscious sedation during endovascular therapy for acute ischemic stroke.

Methods: Through a systematic literature search up to August 2020, 18 studies included 4,802 subjects at baseline with endovascular therapy for acute ischemic stroke and reported a total of 1,711 subjects using general anesthesia and 1,961 subjects using conscious sedation were found. They recorded relationships between the effects of general anesthesia compared to conscious sedation during endovascular therapy for acute ischemic stroke. Odds ratio (OR) or Mean differences (MD) with 95% confidence intervals (CIs) were calculated between the effect of general anesthesia compared to conscious sedation during endovascular therapy for acute ischemic stroke using the dichotomous or contentious methods with a random or fixed-effect model.

Results: No significant difference were found between general anesthesia and conscious sedation during the endovascular therapy for acute ischemic stroke in functional independence at 90 days (OR, 0.78; 95% CI, 0.44-1.40, p = 40); successful recanalization at 24 hr (OR, 1.23; 95% CI, 0.62-2.41, p = 55); mortality at 90 days (OR, 1.36; 95% CI, 0.83-2.24, p = .22); interventional complication (OR, 1.24; 95% CI, 0.76-2.02, p = .40); symptomatic intracranial hemorrhage (OR, 0.64; 95% CI, 0.41-0.99, p = .05); aspiration pneumonia (OR, 0.96; 95% CI, 0.58-1.58, p = .87); and National Institute of Health Stroke Scale score after 24 hr (MD, 0.38; 95% CI, -1.15-1.91, p = .62); with relative relationship favoring general anesthesia only in decreasing the symptomatic intracranial hemorrhage.

Conclusions: General anesthesia has no independent relationship compared to conscious sedation during the endovascular therapy for acute ischemic stroke with a relative relationship favoring general anesthesia only in decreasing the symptomatic intracranial hemorrhage. This relationship encouraged us to recommend either anesthetic strategy during the endovascular therapy for acute ischemic stroke with no possible fear of higher complication.
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http://dx.doi.org/10.1002/brb3.2161DOI Listing
May 2021

Quality of frozen porcine Longissimus lumborum muscles injected with l-arginine and l-lysine solution.

Meat Sci 2021 Sep 28;179:108530. Epub 2021 Apr 28.

Engineering Research Center of Bio-Process, Ministry of Education, Hefei, University of Technology, Hefei 230009, Anhui, PR China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address:

This study investigated the effects of l-arginine and l-lysine on the water holding capacity, shear force, color, and protein denaturation of frozen porcine Longissimus lumborum. Four batches were prepared, each corresponding to samples of an experimental treatment: without a cryoprotective solution, injecting a 0.3% sodium tripolyphosphate and 0.5% NaCl solution, a 0.5% l-arginine solution, or a 0.5% l-lysine solution. The results showed that both l-arginine and l-lysine decreased thawing loss, cooking loss, shear force, L values, b values, and surface hydrophobicity, but they increased pH values, a values, percentages of peak areas for T relaxation times, and Ca-ATPase activity. Additionally, both histological and transmission electron microscopy images showed that l-lysine, and especially l-arginine could inhibit the formation of gaps between fiber bundles, alleviate the disruption of intracellular spaces, and maintain the structural integrity of sarcomeres. Overall, the results showed that both l-arginine and l-lysine hindered the structural damage of muscle fibers during freezing and protected myofibrillar proteins from denaturation, ultimately contributing to superior quality attributes.
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http://dx.doi.org/10.1016/j.meatsci.2021.108530DOI Listing
September 2021

Fabrication of High Organic Solar Cells with a Non-Halogenated Solvent and the Effect of Substituted Groups for "Same-A-Strategy" Material Combinations.

ACS Appl Mater Interfaces 2021 May 28;13(18):21556-21564. Epub 2021 Apr 28.

CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

We report a class of high-voltage organic solar cells (OSCs) processed by the environmentally friendly solvent tetrahydrofuran (THF), where four benzotriazole (BTA)-based p-type polymers (, , , and ) and a BTA-based small molecule are applied as p-type and n-type materials, respectively, according to "Same-A-Strategy" (SAS). The single-junction OSCs based on all four material blends exhibit a high open-circuit voltage () above 1.10 V. We systematically study the impact of the three different substituents (-OCH, -F, -Cl) on the BTA unit of the polymer donors. Interestingly, containing the unsubstituted BTA unit shows the efficient hole transfer and more balanced charge mobilities, thus leading to the highest power conversion efficiency (PCE) of 10.08% with a of 1.11 V and a of 13.68 mA cm. Due to the upshifted highest electron-occupied molecular orbital (HOMO) level and the weak crystallinity of the methoxy-substituted polymer , the resulting device shows the lowest PCE of 7.40% with a slightly decreased of 1.10 V. In addition, after the chlorination and fluorination, the HOMO levels of the donor materials and are gradually downshifted, contributing to increased values of 1.16 and 1.21 V, respectively. Our results prove that an unsubstituted p-type polymer can also afford high voltage and promising performance via non-halogenated solvent processing, which is of great significance for simplifying the synthesis steps and realizing the commercialization of OSCs.
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http://dx.doi.org/10.1021/acsami.1c03757DOI Listing
May 2021

Blood tumor mutation burden can predict the clinical response to immune checkpoint inhibitors in advanced non-small cell lung cancer patients.

Cancer Immunol Immunother 2021 Apr 25. Epub 2021 Apr 25.

Department of Respiratory Diseases, Thoracic Disease Diagnosis and Treatment Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, Zhejiang Province, China.

Background: Tissue tumor mutation burden (tTMB) assessed by whole-exome sequencing (WES), which has been regarded as the gold standard method of tTMB measurement, can predict the clinical benefits of immune checkpoint inhibitors (ICIs). Multiple studies have investigated the feasibility of utilizing large panels to evaluate TMB but have obtained conflicting results. Furthermore, whether blood TMB (bTMB) can also be a predictive biomarker in NSCLC has not been determined.

Methods: Fifty-six advanced NSCLC patients treated with ICIs were enrolled, including an exploratory cohort (n = 42) and a small independent validation cohort (n = 14). Next-generation sequencing was performed on tumor and plasma samples collected prior to ICI treatment using a panel consisting of 520 cancer-related genes (OncoScreen) to evaluate tTMB/bTMB. WES was also performed on tumor samples to serve as references.

Results: A positive correlation between tTMB derived from WES and OncoScreen was observed. OncoScreen-derived tTMB showed a positive correlation with OncoScreen-derived bTMB. Patients with OncoScreen-derived tTMB [Formula: see text] 7 mutations/Mb (p = 0.003) or bTMB [Formula: see text] 11 mutations/Mb (p = 0.0029) had superior progression-free survival (PFS). In the small validation cohort, patients with OncoScreen-derived bTMB [Formula: see text] 11 mutations/Mb exhibited longer PFS (p = 0.192) with a nonsignificant difference. In all 42 patients who had available bTMB and PFS, patients with bTMB [Formula: see text] 11 mutations/Mb had significantly longer PFS (p = 0.011) than those with bTMB [Formula: see text] 11 mutations/Mb.

Conclusion: Our study confirmed the feasibility of using large panels to estimate TMB. We also demonstrated that bTMB can serve as a potential biomarker for predicting the efficacy of ICIs in NSCLC.
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http://dx.doi.org/10.1007/s00262-021-02943-2DOI Listing
April 2021

An NT-3-releasing bioscaffold supports the formation of -modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury.

Bioact Mater 2021 Nov 7;6(11):3766-3781. Epub 2021 Apr 7.

Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, 510080, China.

The mechanism underlying neurogenesis during embryonic spinal cord development involves a specific ligand/receptor interaction, which may be help guide neuroengineering to boost stem cell-based neural regeneration for the structural and functional repair of spinal cord injury. Herein, we hypothesized that supplying spinal cord defects with an exogenous neural network in the NT-3/fibroin-coated gelatin sponge (NF-GS) scaffold might improve tissue repair efficacy. To test this, we engineered -modified neural stem cell (NSC)-derived neural network tissue with robust viability within an NF-GS scaffold. When NSCs were genetically modified to overexpress TrkC, the NT-3 receptor, a functional neuronal population dominated the neural network tissue. The pro-regenerative niche allowed the long-term survival and phenotypic maintenance of the donor neural network tissue for up to 8 weeks in the injured spinal cord. Additionally, host nerve fibers regenerated into the graft, making synaptic connections with the donor neurons. Accordingly, motor function recovery was significantly improved in rats with spinal cord injury (SCI) that received -modified NSC-derived neural network tissue transplantation. Together, the results suggested that transplantation of the neural network tissue formed in the 3D bioactive scaffold may represent a valuable approach to study and develop therapies for SCI.
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http://dx.doi.org/10.1016/j.bioactmat.2021.03.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044869PMC
November 2021

Preparation of Pickering emulsion gels based on κ-carrageenan and covalent crosslinking with EDC: Gelation mechanism and bioaccessibility of curcumin.

Food Chem 2021 Mar 29;357:129726. Epub 2021 Mar 29.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address:

Pickering emulsions stabilized by zein/carboxymethyl dextrin nanoparticles were added to the κ-carrageenan-based gel matrix to prepare emulsion gels via EDC - induced covalent crosslinking. Texture, WHC and freeze-thaw stability of the emulsion gels increased after crosslinking. The Confocal laser scanning microscope and SEM suggested that droplet clusters could be observed in gel with higher concentration of emulsion. The rheological measurements indicated that the viscosity and gel-like structure were relied on crosslinking agent and emulsion concentration. The photothermal stability of curcumin was significantly enhanced after crosslinking. In addition, in vitro digestion study suggested that the bioaccessibility of curcumin in emulsion gel crosslinked was lower compared to emulsion gel without crosslinking agent. These studies might facilitate the preparation of emulsion gels with excellent stability for bioactive compounds delivery in food and pharmaceutical applications.
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http://dx.doi.org/10.1016/j.foodchem.2021.129726DOI Listing
March 2021

A Porphyrin-Involved Benzene-1,3,5-Tricarboxamide Dendrimer (Por-BTA) as a Multifunctional Interface Material for Efficient and Stable Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Mar 18;13(12):14248-14257. Epub 2021 Mar 18.

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.

Surface defects of perovskite films are the major sources of nonradiative recombination which limit the efficiency and stability of perovskite solar cells. Surface passivation represents one of the most efficient strategies to solve this problem. Herein, for the first time we designed a porphyrin-involved benzene-1,3,5-tricarboxamide dendrimer (Por-BTA) as a multifunctional interface material between the interface of the perovskite and the hole-transporting layer (spiro-OMeTAD) for the surface passivation of perovskite films. The results suggested that Por-BTA not only efficiently passivated the perovskite surface defects via the coordination of the exposed Pb with the carbonyl unit and basic sites of pyrrole units in Por-BTA but also improved the interface contact and the charge transfer between the perovskite and spiro-OMeTAD ascribed to the strong intermolecular π-π stacking of Por-BTA. It was shown that the PSC devices with the Por-BTA treatment exhibited improved power conversion efficiency with the champion of 22.30% achieved (21.30% for the control devices), which is mainly attributed to the increased short-circuit current density and fill factor. Interestingly, the stability of moisture for the Por-BTA-treated device was also enhanced compared to those without the Por-BTA treatment. This work presents a promising direction toward the design of multifunctional organic molecules as the interface materials to improve the cell performance of PSCs.
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http://dx.doi.org/10.1021/acsami.1c00146DOI Listing
March 2021

Surgical resection of esophagogastric junction stromal tumor: How to protect the cardiac function.

World J Gastroenterol 2021 Mar;27(9):854-865

Department of Gastric Surgery, Cancer Hospital of China Medical University (Liaoning Cancer Hospital and Institute), Shenyang 110042, Liaoning Province, China.

Background: Various surgical procedures have been described for gastrointestinal stromal tumors (GISTs) at the esophagogastric junction (EGJ) close to the Z-line. However, surgery for EGJ-GIST involving Z-line has been rarely reported.

Aim: To introduce a novel technique called conformal resection (CR) for open resection of EGJ-GIST involving Z-line.

Methods: In this retrospective study, 43 patients having GISTs involving Z-line were included. The perioperative outcomes of patients receiving CR ( = 18) was compared with that of proximal gastrectomy (PG) ( = 25).

Results: CR was successfully performed in all the patients with negative microscopic margins. The mean operative time, time to first passage of flatus, and postoperative hospital stay was significantly shorter in the CR group ( < 0.05), while the intraoperative blood loss was similar in the two groups. The postoperative gastroesophageal reflux as diagnosed by esophageal 24-h pH monitoring and quality of life at 3 mo were significantly in favor of CR compared to PG (both < 0.001). The 5-year disease-free survival between the two groups was similar ( = 0.163). The cut- off value for the determination of CR or PG was 7.0 mm above the Z-line (83.33% sensitivity, 84.00% specificity, 83.72% accuracy).

Conclusion: CR is safe and feasible for EGJ-GIST located within 7.0 mm above the Z-line.
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http://dx.doi.org/10.3748/wjg.v27.i9.854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941860PMC
March 2021

Evaluation of a Custom SNP Panel for Identifying and Rectifying of Misjudged Paternity in Deficiency Cases.

Front Genet 2021 22;12:602429. Epub 2021 Feb 22.

Bio-evidence Sciences Academy, Western China Science and Technology Innovation Harbour, Xi'an Jiaotong University, Xi'an, China.

Parentage testing is routinely performed by genotyping short tandem repeat (STR) through capillary electrophoresis in the present. However, ambiguous or even misjudged paternity based on STRs happens from time to time in cases where only one putative parent is available. We analyzed STR data of 7,818,969 unrelated pairs and 75 close-relative pairs and found that although the probability of a random false match between non-relatives was 4.22 × 10, the incidence of false or ambiguous paternity results between children and first-degree relatives of their true parent was as high as 18.67%. These results highlight the risk of false inclusion of a relative or even non-relatives in parentage testing with STRs. We then validated all ambiguous STR results by targeted sequencing with a custom panel containing 4,830 individual identification single nucleotide polymorphisms (IISNP), found that the ratio of mismatch loci to total SNPs was 1.78-6.95% in close relatives compared with 10.93-13.49% in unrelated pairs. Last, we reported three real cases with undetermined paternity by STRs and rectified them by dissecting with our IISNP panel. These results suggested that high-density IISNP panel can be used to identify and rectify misjudged cases effectively.
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http://dx.doi.org/10.3389/fgene.2021.602429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937934PMC
February 2021

Design and Fabrication of a MEMS Electromagnetic Swing-Type Actuator for Optical Switch.

Micromachines (Basel) 2021 Feb 22;12(2). Epub 2021 Feb 22.

School of Mechanical and Electrical Engineering, Xinyu University, Xinyu 338004, China.

A microelectromechanical systems system (MEMS) electromagnetic swing-type actuator is proposed for an optical fiber switch in this paper. The actuator has a compact size of 5.1 × 5.1 × 5.3 mm, consisting of two stators, a swing disc (rotator), a rotating shaft, and protective covers. Multi-winding stators and a multipole rotator were adopted to increase the output torque of the actuator. The actuator's working principle and magnetic circuit were analyzed. The calculation results show that the actuator's output torque is decisive to the air gap's magnetic flux density between the stators and the swing disc. NiFe alloy magnetic cores were embedded into each winding center to increase the magnetic flux density. A special manufacturing process was developed for fabricating the stator windings on the ferrite substrate. Six copper windings and NiFe magnetic cores were electroplated onto the ferrite substrates. The corresponding six magnetic poles were configured to the SmCo permanent magnet on the swing disc. A magnetizing device with a particular size was designed and fabricated to magnetize the permanent magnet of the swing disc. The actuator prototype was fabricated, and the performance was tested. The results show that the actuator has a large output torque (40 μNm), fast response (5 ms), and a large swing angle (22°).
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http://dx.doi.org/10.3390/mi12020221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927025PMC
February 2021

Two-Dimensional [email protected] Composite for an Extraordinary Long Cycle Lifetime with High Specific Capacity in an Aluminum Battery.

ACS Appl Mater Interfaces 2021 Mar 4;13(10):11822-11832. Epub 2021 Mar 4.

Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

According to the current research, the graphene-like two-dimensional materials present excellent electrochemical performance in aluminum batteries. However, there is less research on emerging two-dimensional materials in aluminum batteries, and the energy storage mechanism is ambiguous. Herein, we modified the two-dimensional few layered TiCT (F-TiCT) with Ag and prepared a composite material [email protected] The results of physical characterization show that Ag is reduced to Ag by Ti ions and is grown on the surface and interlayer of F-TiCT. More importantly, the electrochemical performance of the two-dimensional material [email protected] is studied in an aluminum battery and shows extraordinary long cycle lifetime with high specific capacity. The discharge specific capacity is about 150 mA h g after 2000 cycles at a current density of 0.5 A g. Furthermore, the energy storage mechanism of [email protected] in aluminum batteries is studied, which shows that it is mainly the intercalation/de-intercalation of [AlCl], accompanied by a small amount of Al intercalating/de-intercalating. In addition, density functional theory (DFT) calculations are carried out to study the interaction between [email protected] and [AlCl] and between MXene and [AlCl]. The results show that [AlCl] anions are easier to intercalate/de-intercalate between the layers of TiCO-Ag.
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http://dx.doi.org/10.1021/acsami.0c20808DOI Listing
March 2021

Construction of Pyridine-Based Chiral Ionic Covalent Organic Frameworks as a Heterogeneous Catalyst for Promoting Asymmetric Henry Reactions.

Org Lett 2021 Mar 24;23(5):1748-1752. Epub 2021 Feb 24.

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China.

The difficulties in the separation of products from the reaction mixture and the recovery of the organic cationic ionic liquids (OCILs) catalysts still need to be addressed. Post modification of the pyridine unit in the covalent organic framework (COF) via the formation of pyridinium salts with the chiral bromoacetate led to the chiral ionic COF with OCIL "immobilized", which was utilized as a heterogeneous catalyst for asymmetric Henry reactions with high yield and excellent stereoselectivity.
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http://dx.doi.org/10.1021/acs.orglett.1c00175DOI Listing
March 2021

Side chain engineering of copolymers based on benzotriazole (BTA) and dithieno[2,3-d;2',3'-d']benzo[1,2-b;4,5-b']dithiophenes (DTBDT) enables a high PCE of 14.6.

Nanotechnology 2021 Feb 22. Epub 2021 Feb 22.

National Center for Nanoscience and Technology, Haidian District, 100190, CHINA.

Dithieno[2,3-d;2',3'-d']benzo[1,2-b;4,5-b']dithiophenes (DTBDT) is a kind of prospective candidate for constructing donor-π-acceptor (D-π-A) copolymer donors applied in organic solar cells (OSCs), but restricted for its relatively poor photovoltaic performance compared with benzo[1,2-b;4,5-b']dithiophenes (BDT)-based analogue. Herein, three conjugated polymers (PE51, PE52 and PE53) based DTBDT and benzo[d][1,2,3]triazole (BTA) bearing different length of side alkyl chain unit were synthesized. The change of alkyl chain length can effectively affect energy level distribution, molecular stacking, miscibility and morphology with the non-fullerene acceptor of Y6, resulting in gradually increased open-circuit voltage (VOC) from 0.79 to 0.83 V, improved short-current density (JSC) and fill-factor (FF) reached up to 25.36 mA cm-2 and 71.94% in PE52-based device (with moderate alkyl chain). Impressively, compared with BDT-based analogue J52-Cl, the significantly enhanced crystallinity and intermolecular interaction of PE52 had effectively boosted the charge transport characteristic and optimized the surface morphology, thereby increasing the power conversion efficiency (PCE) from 12.3% to 14.6%, which is the highest PCE among DTBDT-based polymers and BTA-based polymers. Our results show that not only high efficiency could be achieved via using DTBDT as D unit, but also the length of the alkyl chain on BTA has a significant impact on photovoltaic performance for DTBDT-based polymers.
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http://dx.doi.org/10.1088/1361-6528/abe896DOI Listing
February 2021

Investigation of the Formation Mechanism and Curcumin Bioaccessibility of Emulsion Gels Based on Sugar Beet Pectin and Laccase Catalysis.

J Agric Food Chem 2021 Mar 22;69(8):2557-2563. Epub 2021 Feb 22.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.

In this study, modified whey protein hydrolysates (WPH) were obtained after succinic anhydride succinylation and linear dextrin glycation, and emulsion gels were prepared on the basis of unmodified/modified WPH stabilized emulsions with sugar beet pectin (SBP) addition and laccase-catalyzed cross-linking. The influences of emulsifier types and SBP contents on the texture of emulsion gels were estimated. The texture and rheological properties of emulsion gels were characterized. An ideal gel emulsion was formed when the SBP content was 3% (w/w). A uniform network was observed in emulsion gels stabilized by W-L, W-L-S, and W-S-L. In addition, the effect of the emulsifier type on the bioaccessibility of curcumin encapsulated in emulsion gels was investigated and the W-S-L stabilized emulsion gel exhibited the highest curcumin bioaccessibility (65.57%). This study provides a theoretical basis for the development of emulsion gels with different textures by SBP addition and laccase cross-linking as encapsulation delivery systems.
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http://dx.doi.org/10.1021/acs.jafc.0c07288DOI Listing
March 2021

A Replacement Reaction Enabled Interdigitated Metal/Solid Electrolyte Architecture for Battery Cycling at 20 mA cm and 20 mAh cm.

J Am Chem Soc 2021 Mar 17;143(8):3143-3152. Epub 2021 Feb 17.

Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.

Metal anodes represent as a prime choice for the coming generation rechargeable batteries with high energy density. However, daunting challenges including electrode volume variation and inevitable side reactions preclude them from becoming a viable technology. Here, a facile replacement reaction was employed to fabricate a three-dimensional (3D) interdigitated metal/solid electrolyte composite electrode, which not only provides a stable host structure for buffering the volume change within the composite but also prevents side reactions by avoiding the direct contact between active metal and liquid electrolyte. As a proof-of-concept demonstration, a 3D interdigitated zinc (Zn) metal/solid electrolyte architecture was fabricated via a galvanic replacement reaction between Zn metal foil and indium (In) chloride solution followed by electrochemical activation, featuring the interdigitation between metallic Zn and amorphous indium hydroxide sulfate (IHS) with high Zn conductivity (56.9 ± 1.8 mS cm), large Zn transference number (0.55), and high electronic resistivity [(2.08 ± 0.01) × 10 Ω cm]. The as-designed Zn/IHS electrode sustained stable electrochemical Zn plating/stripping over 700 cycles with a record-low overpotential of 8 mV at 1 mA cm and 0.5 mAh cm. More impressively, it displayed cycle-stable performance with low overpotential of 10 mV under ultrahigh current density and areal capacity (20 mA cm, 20 mAh cm), which outperformed all the reported Zn metal electrodes in mild aqueous electrolyte. The fabrication of interdigitated metal/solid electrolyte was generalized to other metal pairs, including Zn/Sn and Zn/Co, which provide inspiration for next-generation Zn metal batteries with high energy density and reversibility.
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http://dx.doi.org/10.1021/jacs.0c11753DOI Listing
March 2021

Development and verification of an immune-related gene pairs prognostic signature in ovarian cancer.

J Cell Mol Med 2021 Mar 4;25(6):2918-2930. Epub 2021 Feb 4.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Ovarian cancer (OV) is the most common gynaecological cancer worldwide. Immunotherapy has recently been proven to be an effective treatment strategy. The work here attempts to produce a prognostic immune-related gene pair (IRGP) signature to estimate OV patient survival. The Gene Expression Omnibus (GEO) and Cancer Genome Atlas (TCGA) databases provided the genetic expression profiles and clinical data of OV patients. Based on the InnateDB database and the least absolute shrinkage and selection operator (LASSO) regression model, we first identified a 17-IRGP signature associated with survival. The average area under the curve (AUC) values of the training, validation, and all TCGA sets were 0.869, 0.712, and 0.778, respectively. The 17-IRGP signature noticeably split patients into high- and low-risk groups with different prognostic outcomes. As suggested by a functional study, some biological pathways, including the Toll-like receptor and chemokine signalling pathways, were significantly negatively correlated with risk scores; however, pathways such as the p53 and apoptosis signalling pathways had a positive correlation. Moreover, tumour stage III, IV, grade G1/G2, and G3/G4 samples had significant differences in risk scores. In conclusion, an effective 17-IRGP signature was produced to predict prognostic outcomes in OV, providing new insights into immunological biomarkers.
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http://dx.doi.org/10.1111/jcmm.16327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957197PMC
March 2021

Baseline sensitivity and fungicidal action of propiconazole against Penicillium digitatum.

Pestic Biochem Physiol 2021 Feb 19;172:104752. Epub 2020 Nov 19.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Green mold, caused by Penicillium digitatum, is the most important citrus postharvest disease worldwide and often causes substantial economic losses to the citrus industry. The demethylation inhibitor (DMI) fungicides are highly effective against a broad range of fungal pathogens, but the DMI fungicide propiconazole has not been registered yet in China for the control of citrus green mold. In this study, baseline sensitivity of P. digitatum to propiconazole was determined. The frequency distribution of logarithms of EC values for 118 isolates collected from five regions in China was bimodal, and among the 118 isolates, 18 isolates were less sensitive or had low resistance to propiconazole. The mean EC value of the sensitive 100 isolates was 0.104 mg/L. Preventive control efficacies on Satsuma mandarin for propiconazole at 200 and 400 mg/L were 63.1 and 84.3%, respectively. The fruit treated with propiconazole at 40 and 100 mg/L produced significantly fewer conidia, and the virulence of the conidia decreased by 12.3 and 14.8%, respectively. Studies with propidium iodide showed that the membrane integrity was damaged for 25.6% of conidia produced on PDA amended with propiconazole at 0.1 mg/L. Fluorescence microscopy observations of P. digitatum conidia stained with 2,7-dichlorofluorescin showed that propiconazole significantly induced the generation of intracellular reactive oxygen species (ROS). Compared with the sensitive isolates, no point mutations were detected in either the coding or promoter region of the target gene CYP51A of the isolates with low resistance to propiconazole. However, the relative expression levels of CYP51A for three resistant isolates were higher than sensitive isolates, and the mean relative expression was 2.08 for resistant isolates versus 0.62 for sensitive isolates in the absence of propiconazole and 3.12 versus 1.44 in the presence of propiconazole. These results indicate increased expression of CYP51A is the molecular mechanism for low resistance of P. digitatum to propiconazole.
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http://dx.doi.org/10.1016/j.pestbp.2020.104752DOI Listing
February 2021

MCM3AP-AS1 promotes cisplatin resistance in gastric cancer cells via the miR-138/FOXC1 axis.

Oncol Lett 2021 Mar 18;21(3):211. Epub 2021 Jan 18.

Department of Digestive Internal Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu 221000, P.R. China.

The dysregulation of long non-coding RNAs (lncRNAs) serves a pivotal role in the pathogenesis and development of multiple types of human cancer, including gastric cancer (GC). MCM3AP-antisense 1 (MCM3AP-AS1) has been reported to function as a tumor promoter in various types of cancer. However, the biological function of MCM3AP-AS1 in the resistance of GC cells to cisplatin (CDDP) remains to be elucidated. The present study aimed to elucidate the mechanisms of MCM3AP-AS1 in the resistance of GC cells to CDDP. The expression levels of MCM3AP-AS1, miR-138 and FOXC1 were measured via reverse transcription-quantitative PCR. In addition, cell viability, migration and invasion were assessed via the Cell Counting Kit-8, wound healing and transwell assays, respectively. The interaction between genes was confirmed via the dual-luciferase reporter and pull-down assays. Western blot analysis was performed to detect FOXC1 protein expression. In the present study, it was demonstrated that MCM3AP-AS1 expression was upregulated in CDDP-resistant GC cells and that MCM3AP-AS1-knockdown suppressed CDDP resistance in GC cells. Moreover, the examination of the molecular mechanism indicated that MCM3AP-AS1 upregulated FOXC1 expression by sponging microRNA (miR)-138. Additionally, it was identified that the overexpression of FOXC1 abolished MCM3AP-AS1-knockdown- or miR-138 mimic-mediated inhibitory effects on CDDP resistance in GC cells. In conclusion, the present findings suggested that MCM3AP-AS1 enhanced CDDP resistance by sponging miR-138 to upregulate FOXC1 expression, indicating that MCM3AP-AS1 may be a novel promising biomarker for the diagnosis and treatment of patients with GC.
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http://dx.doi.org/10.3892/ol.2021.12472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836396PMC
March 2021

Hexagonal metal oxide monolayers derived from the metal-gas interface.

Nat Mater 2021 Jan 18. Epub 2021 Jan 18.

School of Engineering, RMIT University, Melbourne, Victoria, Australia.

Two-dimensional (2D) crystals are promising materials for developing future nano-enabled technologies. The cleavage of weak, interlayer van der Waals bonds in layered bulk crystals enables the production of high-quality 2D, atomically thin monolayers. Nonetheless, as earth-abundant compounds, metal oxides are rarely accessible as pure and fully stoichiometric monolayers owing to their ion-stabilized 'lamellar' bulk structure. Here, we report the discovery of a layered planar hexagonal phase of oxides from elements across the transition metals, post-transition metals, lanthanides and metalloids, derived from strictly controlled oxidation at the metal-gas interface. The highly crystalline monolayers, without the support of ionic dopants or vacancies, can easily be mechanically exfoliated by stamping them onto substrates. Monolayer and few-layered hexagonal TiO are characterized as examples, showing p-type semiconducting properties with hole mobilities of up to 950 cm V s at room temperature. The strategy can be readily extended to a variety of elements, possibly expanding the exploration of metal oxides in the 2D quantum regime.
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http://dx.doi.org/10.1038/s41563-020-00899-9DOI Listing
January 2021