Publications by authors named "Bai Gao"

11 Publications

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The protective effect of HOXA5 on carotid atherosclerosis occurs by modulating the vascular smooth muscle cell phenotype.

Mol Cell Endocrinol 2021 Jun 11;534:111366. Epub 2021 Jun 11.

Department of Vascular Surgery, The First Hospital of China Medical University, Shenyang, China. Electronic address:

The phenotypic change of vascular smooth muscle cells (VSMCs) from a contractile to a synthetic form is a key player in atherogenic processes. Homeobox A5 (HOXA5), a transcription factor of the homeobox gene family, has been shown to regulate cell differentiation and morphogenesis. The present study was designed to clarify the involvement of HOXA5 in VSMC phenotypic transition in carotid atherosclerosis (CAS). Activated VSMCs in vitro and ApoE mice in vivo were employed to determine HOXA5's function. Results showed that both the mRNA and protein expression levels of HOXA5 were decreased in platelet-derived growth factor-BB (PDGF-BB)-induced VSMCs. Overexpression of HOXA5 suppressed VSMC conversion from a contractile to a synthetic type in the presence of PDGF-BB, as evidenced by increased contractile markers (calponin, α-SMA and SM22α) along with decreased synthetic markers (vimentin, PCNA and thrombospondin). PDGF-BB-induced proliferation and migration of VSMCs were recovered by HOXA5. Knockdown of HOXA5 had the opposite effect on VSMCs. In vivo, a CAS model was established using ApoE mice fed with a Western-type diet and placing a perivascular carotid collar. We observed a significant reduction in HOXA5 in the carotid arteries of CAS mice. Similar to the in vitro results, HOXA5 overexpression reduced neointimal hyperplasia and plaque formation and inhibited VSMC dedifferentiation and migration. Furthermore, PPARγ was also downregulated in vitro and in vivo, and its antagonist GW9662 reversed HOXA5-mediated inhibition of VSMC dedifferentiation and migration. In summary, we suggest that HOXA5 protects against CAS progression by inhibiting VSMC dedifferentiation through activation of PPARγ.
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http://dx.doi.org/10.1016/j.mce.2021.111366DOI Listing
June 2021

HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1.

IUBMB Life 2021 Jun 11. Epub 2021 Jun 11.

Department of Vascular Surgery, The First Hospital of China Medical University, Shenyang, China.

Macrophage polarization is of great importance in the formation of atherosclerotic plaque. Homeobox A5 (HOXA5), one of the homeobox transcription factors, has been revealed to be closely associated with macrophage phenotype switching. This study aims to investigate the role of HOXA5 in carotid atherosclerosis (CAS). Herein, the role of HOXA5 was explored in polarized RAW264.7 macrophages in vitro and ApoE mice in vivo. Interestingly, compared with that in M0 macrophages, both the mRNA and protein expression levels of HOXA5 were decreased in lipopolysaccharide (LPS)/interferon (IFN)-γ-induced M1 macrophages, while increased in IL-4-induced M2 macrophages. In addition, in the presence of IL-4, HOXA5-overexpressing RAW264.7 cells preferred to polarizing toward M2 phenotypes. Furthermore, we found that HOXA5 bound to the promoter region and activated the expression of mediator subunit 1 (MED1), a gene known to regulate macrophage differentiation. Knocking MED1 down inhibited HOXA5-enhanced M2 macrophage polarization. In vivo, the CAS model was induced in ApoE mouse fed with a Western-type diet and placed a perivascular carotid collar. Decreased mRNA and protein expressions of HOXA5 were observed in carotid arteries of CAS mice. Forced overexpression of HOXA5 reduced intimal hyperplasia and lipid accumulation in carotid vessels, and it also promoted the polarization of macrophages to M2 subtypes. The expression of MED1 was decreased in atherosclerotic carotid vessels, while HOXA5 overexpression restored its change. Collectively, HOXA5 in carotid arteries is involved in the macrophage M1/M2 switching in atherosclerotic plaque, which may be associated with its transcriptional regulation of MED1.
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http://dx.doi.org/10.1002/iub.2515DOI Listing
June 2021

Association between plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism and risk of Alzheimer's disease, metabolic syndrome, and female infertility: A meta-analysis.

Medicine (Baltimore) 2020 Dec;99(50):e23660

Department of Neurology, Shenyang First People's Hospital, Dadong District.

Background: Plasminogen activator inhibitor-1 (PAI-1) is considered to be involved in the physiopathological mechanisms of Alzheimer's disease (AD), metabolic syndrome (MetS), and female infertility. Previous studies investigating the association between PAI-14G/5G (rs1799889) gene polymorphism and the risk of AD, MetS, and female infertility have reported inconsistent results. The aim of the present study was to investigate possible associations.

Methods: Eligible studies were retrieved through PubMed, Medline, EMBASE, CNKI, and WANFANG databases. The odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the associations. Subgroup analyses by ethnicity and mean age, sensitivity analyses, and publication bias were performed.

Results: Five studies (four articles) for AD, six studies (six articles) for MetS, and four studies (four articles) for female infertility were included in this meta-analysis. Our results showed no significant associations between the PAI-14G/5G polymorphism and the risk of AD and female infertility in five genetic models. For the risk of MetS, the PAI-1 4G/5G (rs1799889) polymorphism may be associated with the risk of MetS (4G vs 5G, OR = 1.31, 95%CI = 1.04-1.64, P = .021), especially in Asians (4G/4G vs 4G/5G+5G/5G, OR = 1.38, 95%CI = 1.01-1.87, P = .041) and patients with mean age > 50 years old (4G/4G vs 4G/5G+5G/5G, OR = 1.36, 95%CI = 1.03-1.78, P = .029).

Conclusion: The present meta-analysis suggested that the PAI-1 4G/5G polymorphism might be associated with the risk of MetS, but no evidence was detected for AD and female infertility.
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http://dx.doi.org/10.1097/MD.0000000000023660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738113PMC
December 2020

No association between the vitamin D-binding protein (DBP) gene polymorphisms (rs7041 and rs4588) and multiple sclerosis and type 1 diabetes mellitus: A meta-analysis.

PLoS One 2020 12;15(11):e0242256. Epub 2020 Nov 12.

Department of Neurology, Shenyang First People's Hospital, Shenyang, Liaoning Province, China.

Background: The association between polymorphisms in vitamin D-binding protein (DBP) gene and the risk of multiple sclerosis (MS) and type 1 diabetes mellitus (T1DM) has been investigated in many studies, but the studies showed controversial results. The rationale for this meta-analysis was to determine whether DBP polymorphisms increases the risk of MS and T1DM by pooling data.

Methods: Potentially relevant studies were searched using GWAS Catalog, PubMed, Embase, CNKI and WANFANG databases up to November 2019. The pooled odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were performed to estimate the associations in a fixed-effects or random-effects model.

Results: A total of 13 studies were enrolled in this meta-analysis, including eight studies for MS and five for T1DM. The overall results showed that there was no significant association of DBP rs7041 and rs4588 polymorphisms with the risk of MS and T1DM under any genetic model. Similarly, subgroup analysis by ethnicity revealed that no significant association of rs7041 and rs4588 polymorphisms with the risk of MS and T1DM was observed in white or non-white racial groups.

Conclusions: This meta-analysis provides evidence that DBP rs7041 and rs4588 polymorphisms may not be associated with an increased risk in MS and T1DM. However, these findings need further validation by larger-scale epidemiological studies and genome-wide association studies (GWASs) in different populations.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242256PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7661054PMC
January 2021

Photocatalytic decontamination of tetracycline and Cr(VI) by a novel α-FeOOH/FeS photocatalyst: One-pot hydrothermal synthesis and Z-scheme reaction mechanism insight.

J Hazard Mater 2020 10 26;397:122580. Epub 2020 Apr 26.

State Key Laboratory of Nuclear Resources and Environment, East China University of Technology, Nanchang, 330013, China; School of Water Resources & Environmental Engineering, East China University of Technology, Nanchang, 330013, China. Electronic address:

Tetracycline and Cr(VI) as non-biodegradable environmental contaminants have attracted increasing attention because of their chronic toxicity. In this regard, the environmentally friendly Z-scheme photocatalytic decontamination system has been widely used for contaminant treatment. Herein, a novel 3D Z-scheme α-FeOOH/FeS composite photocatalyst was successfully synthesized for the first time via a simple one-pot hydrothermal method. X-ray diffraction (XRD) and Fourier-transform infrared (FT-IR) analyses and high-resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS) demonstrated that the O component of the heterogeneous nanostructures formed by the FeOFe linkages in α-FeOOH was replaced by S to generate FeSFe linkages in the resulting FeS. As expected, the novel 3D Z-scheme α-FeOOH/FeS composites exhibited remarkable photocatalytic activity for Cr(VI) reduction and tetracycline degradation compared to pure α-FeOOH. Photoluminesence (PL) measurement and electrochemical impedance spectroscopy (EIS), as well as density functional theory (DFT) calculations, suggested that the enhanced photocatalytic activity of the Z-scheme α-FeOOH/FeS composite can be attributed to the improved photo-absorption properties and the effective separation of photo-induced charge carriers caused by the Z-scheme system of the as-prepared 3D α-FeOOH/FeS composites. Thus, this work may facilitate the effective design of α-FeOOH-based photocatalysts.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122580DOI Listing
October 2020

Occurrence and Distribution of Uranium in a Hydrological Cycle around a Uranium Mill Tailings Pond, Southern China.

Int J Environ Res Public Health 2020 01 26;17(3). Epub 2020 Jan 26.

Fundamental Science on Radioactive Geology and Exploration Technology Laboratory, East China University of Technology, NanChang, Jiangxi, 330013, China.

Uranium (U) mining activities, which lead to contamination in soils and waters (i.e., leachate from U mill tailings), cause serious environmental problems. However, limited research works have been conducted on U pollution associated with a whole soil-water system. In this study, a total of 110 samples including 96 solid and 14 water samples were collected to investigate the characteristics of U distribution in a natural soil-water system near a U mining tailings pond. Results showed that U concentrations ranged from 0.09 ± 0.02 mg/kg to 2.56 × 10± 23 mg/kg in solid samples, and varied greatly in different locations. For tailings sand samples, the highest U concentration (2.56× 10 ± 23 mg/kg) occurred at the depth of 80 cm underground, whereas, for paddy soil samples, the highest U concentration (5.22 ± 0.04 mg/kg) was found at surface layers. Geo-accumulation index and potential ecological hazard index were calculated to assess the hazard of U in the soils. The calculation results showed that half of the soil sampling sites were moderately polluted. For groundwater samples, U concentrations ranged from 0.55 ± 0.04 mg/L to 3.36 ± 0.02 mg/L with a mean value of 2.36 ± 0.36 mg/L, which was significantly lower than that of percolating waters (ranging from 4.56 ± 0.02 mg/L to 12.05 ± 0.04 mg/L, mean 7.91 ± 0.98 mg/L). The results of this study suggest that the distribution of U concentrations in a soil-water system was closely associated with hydrological cycles and U concentrations decreased with circulation path.
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http://dx.doi.org/10.3390/ijerph17030773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037307PMC
January 2020

Characteristics and Assessment of Toxic Metal Contamination in Surface Water and Sediments Near a Uranium Mining Area.

Int J Environ Res Public Health 2020 01 15;17(2). Epub 2020 Jan 15.

State Key Laboratory of Nuclear Resources and Environment, College of Water Resources and Environmental Engineering, East China University of Technology, Nanchang 330013, China.

Concentrations of potentially toxic metals including Cd, Cu, Pb, Cr, U, Th in surface water and sediment samples collected from a river were analyzed to assess the contaminations, distribution characteristics, and sources of these metals. The contents of the metals were lower than the standard levels set by World Health Organization (WHO) for drinking water. However, U and Th contents were far beyond the background values of surface water. The concentrations of Cd, Cr, and U in sediments were higher than the background values and the Probable Effect Level (PEL) of sediment quality guidelines (SQGs) which may result in high potential harmful biological effects to aquatic ecosystems. Based on the contamination factor (CF), geo-accumulation index (I), and potential ecological risk index (RI), Cd, Cr, and U were considered to be the metals that mainly contribute to the contamination of sediments. The calculation results also indicated that the sites adjacent to the uranium ore field were highly polluted. Results of cluster analysis, principal component analysis, and correlation analysis revealed that Cr, Pb, U, and Th were highly correlated with each other. These metals mainly originated from both anthropogenic sources and natural processes, especially emissions from uranium mining and quarrying, whereas Cd mostly came from anthropogenic sources (agricultural activities) of the upper reaches of the river.
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http://dx.doi.org/10.3390/ijerph17020548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7014452PMC
January 2020

[Feasible study of carotid artery Doppler ultrasound blood flow measurement during chest compression cardiopulmonary resuscitation].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2019 Mar;31(3):309-312

Department of Critical Care Medicine, Zhengzhou People's Hospital, Zhengzhou 450003, Henan, China.

Objective: To determine the feasibility of ultrasound to measure blood flow on patients with chest compression cardiopulmonary resuscitation (CPR), and to find out a real-time, noninvasive hemodynamic evaluation method.

Methods: A prospective study was conducted. All adult patients undergoing CPR admitted to Department of Emergency and intensive care unit (ICU) of Zhengzhou People's Hospital from May 2016 to November 2018 were enrolled. The blood flow over the right carotid arteries during chest compressions was recorded with a bedside ultrasound machine. The peak systolic flow velocity (PSV) and end diastolic flow velocity (EDV) of carotid artery were recorded at 1 minute after the start of CPR and 1 minute before the end of CPR. The mean compression frequency during the whole recovery period was recorded, the rate of compression reaching the standard was evaluated by ultrasound (the rate of compression 100-120 times/min was defined as up to standard), and the interruption time of compression was calculated retrospectively according to the ultrasound image data recorded during CPR.

Results: Thirty-nine patients were enrolled, and 21 patients were successfully rescued, with a successful rate of 53.8%, the time of restoration of spontaneous circulation (ROSC) was (10.9±5.3) minutes. The time from CPR to retrieve an ultrasound image was 1.1-4.9 minutes, with an average of (2.5±1.2) minutes. Satisfactory ultra-sonographic images were obtained in 28 patients during the whole course of chest compression. The acquisition rate was 71.8% (28/39). In the process of compression, if the frequency of compression was less than 100 times/min or the velocity of carotid artery dropped (PSV < 30 cm/s), the chest compressors should be reminded and corrected in time. The PSV at 1 minute after CPR start of 28 patients with satisfactory ultrasound images was (62.9±18.5) cm/s, and the EDV was (13.9±3.5) cm/s, the PSV at 1 minute before the end of CPR was (55.4±18.4) cm/s, and the EDV was (12.9±3.7) cm/s. There was no significant difference in above parameters between the two time points (both P > 0.05), suggesting that satisfactory resuscitation effect was achieved in the whole process of CPR. The compression frequency of 28 patients was 100-149 times/min with an average of (117±47) times/min. The rate of compression with standard was 85.7% (24/28), and the total interruption time of compression accounted for 4.4% of all compression time (25.9 minutes/587.2 minutes).

Conclusions: Ultrasound measurement of common carotid artery blood flow during CPR has the advantage of real-time and non-invasive, and it is feasible in clinical work.
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http://dx.doi.org/10.3760/cma.j.issn.2095-4352.2019.03.010DOI Listing
March 2019

Shallow groundwater quality and associated non-cancer health risk in agricultural areas (Poyang Lake basin, China).

Environ Geochem Health 2018 Oct 24;40(5):2223-2242. Epub 2018 Mar 24.

East China University of Technology, 418 Guanglan Avenue, Nanchang, 330013, China.

Owing to their accessibility, shallow groundwater is an essential source of drinking water in rural areas while usually being used without control by authorities. At the same time, this type of water resource is one of the most vulnerable to pollution, especially in regions with extensive agricultural activity. These factors increase the probability of adverse health effects in the population as a result of the consumption of shallow groundwater. In the present research, shallow groundwater quality in the agricultural areas of Poyang Lake basin was assessed according to world and national standards for drinking water quality. To evaluate non-cancer health risk from drinking groundwater, the hazard quotient from exposure to individual chemicals and hazard index from exposure to multiple chemicals were applied. It was found that, in shallow groundwater, the concentrations of 11 components (NO, NH, Fe, Mn, As, Al, rare NO, Se, Hg, Tl and Pb) exceed the limits referenced in the standards for drinking water. According to the health risk assessment, only five components (NO, Fe, As, rare NO and Mn) likely provoke non-cancer effects. The attempt to evaluate the spatial distribution of human health risk from exposure to multiple chemicals shows that the most vulnerable area is associated with territory characterised by low altitude where reducing or near-neutral conditions are formed (lower reaches of Xiushui and Ganjiang Rivers). The largest health risk is associated with the immune system and adverse dermal effects.
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http://dx.doi.org/10.1007/s10653-018-0094-zDOI Listing
October 2018

Sources and behaviour of nitrogen compounds in the shallow groundwater of agricultural areas (Poyang Lake basin, China).

J Contam Hydrol 2017 07 17;202:59-69. Epub 2017 May 17.

East China University of Technology, 418 Guanglan Avenue, Nanchang 330013, China.

Nitrogen contamination of natural water is a typical problem for various territories throughout the world. One of the regions exposed to nitrogen pollution is located in the Poyang Lake basin. As a result of agricultural activity and dense population, the shallow groundwater of this area is characterised by a high concentration of nitrogen compounds, primarily NO, with the concentration varying from 0.1mg/L to 206mg/L. Locally, high ammonium content occurs in the shallow groundwater with low reduction potential Eh (<100mV). However, in general, the shallow groundwater of the Poyang Lake basin has Eh>100mV. To identify sources of nitrogen species and the factors that determine their behaviour, the dual stable isotope approach (δN and δО) and physical-chemical modelling were applied. Actual data were collected by sampling shallow groundwater from domestic water supply wells around the lake. The δО values from -4.1‰ to 13.9‰ with an average value of 5.3 permille indicate a significant influence of nitrification on nitrogen balance. The enrichment of nitrate with the N isotope indicates that manure and domestic sewage are the principal sources of nitrogen compounds. Inorganic nitrogen speciation and thermodynamic calculations demonstrate the high stability of nitrate in the studied groundwater. Computer simulation and field observations indicate the reducing conditions formed under joint effects of anthropogenic factors and appropriate natural conditions, such as the low-level topography in which decreased water exchange rate can occur. The simulation also demonstrates the growth in pH of the groundwater as a consequence of fertilisation, which, in turn, conduced to the clay mineral formation at lower concentrations of aqueous clay-forming components than the ones under the natural conditions.
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http://dx.doi.org/10.1016/j.jconhyd.2017.05.002DOI Listing
July 2017

AgPO/rectorite nanocomposites: Ultrasound-assisted preparation, characterization and enhancement of stability and visible-light photocatalytic activity.

Ultrason Sonochem 2017 01 25;34:831-838. Epub 2016 Jul 25.

School of Water Resources & Environmental Engineering, East China University of Technology, Nanchang 330013, China.

To overcome the drawback of low stable brought by the transformation of Ag into Ag, a highly efficient and stable photocatalyst AgPO/rectorite composite was successfully synthesized by ultrasound-assisted precipitation method. The as-prepared samples were characterized by field-emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, N adsorption-desorption, room-temperature photoluminescence spectra, Fourier transform infrared spectrum measurements and UV-vis diffuse reflectance spectra. The absorption edges of the AgPO/rectorite display a noticeable shift to the visible light region as compared to that of the AgPO. Compared with bare AgPO, the AgPO/rectorite composite by ultrasound-assisted precipitation process exhibits significantly enhanced photocatalytic activity and stable for methyl orange (MO) degradation under visible light irradiation. The improved activity of the AgPO/rectorite photocatalyst could be attributed to the expanded visible light absorption, the enhanced interfacial charge transfer and the inhibited recombination of electron-hole pairs. Therefore, the facile ultrasound-assisted preparation process provides some insight into the application of AgPO/rectorite nanocomposites in photocatalytic degradation of organic pollutants.
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http://dx.doi.org/10.1016/j.ultsonch.2016.07.017DOI Listing
January 2017